WO1990007112A1 - Procede de determination de l'activite biologique d'un sol - Google Patents

Procede de determination de l'activite biologique d'un sol Download PDF

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Publication number
WO1990007112A1
WO1990007112A1 PCT/FR1989/000674 FR8900674W WO9007112A1 WO 1990007112 A1 WO1990007112 A1 WO 1990007112A1 FR 8900674 W FR8900674 W FR 8900674W WO 9007112 A1 WO9007112 A1 WO 9007112A1
Authority
WO
WIPO (PCT)
Prior art keywords
soil
alkaline phosphatase
expressed
plotted
enzymatic activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR1989/000674
Other languages
English (en)
French (fr)
Inventor
Claude Bourguignon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to DE68918599T priority Critical patent/DE68918599D1/de
Priority to EP90900902A priority patent/EP0449924B1/fr
Publication of WO1990007112A1 publication Critical patent/WO1990007112A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/24—Earth materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/10—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases p-Nitrophenol derivatives

Definitions

  • another so-called characteristic with which the measurement of alkaline phosphatase is correlated is the cation exchange capacity of the soil.
  • c2.Calibration curve (colored standard solution): 1 ml of a standard solution of p-nitrophenol (lg / 1) is diluted in 100 ml of water and the resulting dilution is distributed in Erlenmeyer flasks, to reason respectively 0, 1, 2, 3, 4 and 5 ml. Adjust to 5 ml with water, which gives a range of concentrations from 0 to 50 ⁇ g of p-nitrophenol. The absorption of this range is measured at 410 ⁇ m and the corresponding curve is plotted. c3.test socket
  • the measurement used is that of the technique known as "methylene blue” from the Central Laboratory of Bridges and Roads.
  • the assay consists in making additions of methylene blue (10 g / l) in a suspension containing the test portion until the particles of the soil are covered with a monomolecular layer of this dye. a - Preparation of the soil sample
  • a range of glucose is prepared from a mother solution of glucose at 10 g / l (therefore at 4 g of carbon per liter). dilutions titrating 0, 10, 20, 60, 100, 200, 300, 400, 500 and 600 ⁇ g of carbon / ml respectively. The absorption of this range is measured under 625 ⁇ m and the corresponding calibration curve is plotted.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Life Sciences & Earth Sciences (AREA)
  • Geology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Environmental & Geological Engineering (AREA)
  • Microbiology (AREA)
  • Remote Sensing (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
PCT/FR1989/000674 1988-12-22 1989-12-22 Procede de determination de l'activite biologique d'un sol Ceased WO1990007112A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE68918599T DE68918599D1 (de) 1988-12-22 1989-12-22 Verfahren zur bestimmung der biologischen aktivität eines bodens.
EP90900902A EP0449924B1 (fr) 1988-12-22 1989-12-22 Procede de determination de l'activite biologique d'un sol

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8817008A FR2640998B1 (enExample) 1988-12-22 1988-12-22
FR88/17008 1988-12-22

Publications (1)

Publication Number Publication Date
WO1990007112A1 true WO1990007112A1 (fr) 1990-06-28

Family

ID=9373294

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR1989/000674 Ceased WO1990007112A1 (fr) 1988-12-22 1989-12-22 Procede de determination de l'activite biologique d'un sol

Country Status (6)

Country Link
EP (1) EP0449924B1 (enExample)
AU (1) AU4834290A (enExample)
DE (1) DE68918599D1 (enExample)
ES (1) ES2065520T3 (enExample)
FR (1) FR2640998B1 (enExample)
WO (1) WO1990007112A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111850092A (zh) * 2020-07-01 2020-10-30 甘肃省地质矿产勘查开发局第三地质矿产勘查院(甘肃遥感地质中心、甘肃地质灾害防治工程勘查设计院、甘肃省地质遗迹评估中心、甘肃省中心实验室) 基于土壤酶活性测定的土壤生物学活性和生产力评价方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0085348A1 (en) * 1982-01-22 1983-08-10 American Hoechst Corporation Reagents for the determination of enzymes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0085348A1 (en) * 1982-01-22 1983-08-10 American Hoechst Corporation Reagents for the determination of enzymes

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Biological Abstracts, Volume 78, No. 9, 1989, (Philadelphia, PA, US), W.A. DICK: "Influence of Long-Term Tillage and Crop Rotation Combinations on Soil Enzyme Acitvities", voir pages 7257-7258, Resume 64394, & Soil Sci Soc Am J 48(3): 569-574 *
Biological Abstracts, Volume 87, no. 7, 1989, (Philadelphia, PA, US), T. SUTTNER et al.: "Correlation Between the Arginine Ammonification, Enzyme Activities, Microbial Biomass, Physical and Chemical Properties of Different Soils", voir page AB-1102, Resume 77236, & Zentralbl Mikrobiol 143(8): 569-573, 1988 *
CHEMICAL ABSTRACTS, Volume 94, No. 5, 2 Fevrier 1981, (Columbus, Ohio, US), P. NANNIPIERI et al.: "Extraction of Phosphatase, Urease, Proteases, Organic Carbon, and Nitrogen from Soil", voir page 451* Resume 29252k, & Soil Sci. Soc. Am. J. 1980, 44(5), 1011-16* *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111850092A (zh) * 2020-07-01 2020-10-30 甘肃省地质矿产勘查开发局第三地质矿产勘查院(甘肃遥感地质中心、甘肃地质灾害防治工程勘查设计院、甘肃省地质遗迹评估中心、甘肃省中心实验室) 基于土壤酶活性测定的土壤生物学活性和生产力评价方法

Also Published As

Publication number Publication date
FR2640998B1 (enExample) 1994-06-10
EP0449924B1 (fr) 1994-09-28
AU4834290A (en) 1990-07-10
ES2065520T3 (es) 1995-02-16
EP0449924A1 (fr) 1991-10-09
DE68918599D1 (de) 1994-11-03
FR2640998A1 (enExample) 1990-06-29

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