WO1988008972A1 - Systeme mettant en oeuvre un capteur biologique pour controler une caracteristique d'un constituant selectionne dans un milieu - Google Patents

Systeme mettant en oeuvre un capteur biologique pour controler une caracteristique d'un constituant selectionne dans un milieu Download PDF

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Publication number
WO1988008972A1
WO1988008972A1 PCT/US1988/001494 US8801494W WO8808972A1 WO 1988008972 A1 WO1988008972 A1 WO 1988008972A1 US 8801494 W US8801494 W US 8801494W WO 8808972 A1 WO8808972 A1 WO 8808972A1
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WO
WIPO (PCT)
Prior art keywords
component
binding
characteristic
detection
sensor
Prior art date
Application number
PCT/US1988/001494
Other languages
English (en)
Inventor
Peter Wing-Poon Cheung
Edward Bakewell Weiler
Clement Eugene Furlong, Jr.
Original Assignee
The Washington Technology Center
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Washington Technology Center filed Critical The Washington Technology Center
Publication of WO1988008972A1 publication Critical patent/WO1988008972A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4145—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors

Definitions

  • the operation of the discrete sensor element 16 is controlled, in part, by instructions introduced through a user input, such as a keypad 22, provided on the instrument console 12.
  • a user input such as a keypad 22, provided on the instrument console 12.
  • a particular characteristic of the component of interest is monitored via console 12 by the analysis of signals from sensor element 16, as described below.
  • the information produced by console 12 is then displayed to the operator via a display 24.
  • the means for immobilizing a binding component 32 on semiconducting element 34 depends on the composition of semiconducting element 34.
  • the silicon dioxide surface is preferably derivatized using vapor phase deposition of silane compounds, such as 3-aminoprophyltriethoxysilane (APTES). Vapor phase deposition in this manner deposits a chemically active layer of the silane compound covalently bonded to the SIO2 surface.
  • the surface can be derivatized using liquid phase deposition.
  • a binding protein, selected from those previously described, is then immobilized on the derivatized surface of the semiconductor by a covalent bond.
  • the final section of sensor element 16 to be discussed is the binding reversal element 38.
  • the particular binding element 32 selected can be induced to release the component bound to the insulative material 50 by increasing the temperature of the gate 52 to approximately 75°C.
  • diffused or thin-film resistors 58 can be integrated with the other components or the sensor element 16. The number, location, and resistivity of resistors 58, as well as the voltage applied, determines their power loss and, hence, the heat transferred to sensor element 16.
  • a system 10 constructed in accordance with this invention does not require the use of external standard calibration references. More particularly, given the precise nature of the KQ of the binding elements 32, the relationship between the output of sensor element 16 and the concentration of the component in solution does not require adjustment between sensors.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Engineering & Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Electrochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Un instrument de mesure possède une protéine à liaison sélective réversible immobilisée sur la région de la gâchette isolée d'un transistor à effet de champ (IGFET) disposé sur un capteur. Lorsque ce capteur est immergé dans une solution, la protéine lie à la gâchette un constituant choisi de la solution, produisant ainsi un effet sur un courant circulant à travers le IGFET. Une pluralité de ces agencements protéine de liaison-IGFET peuvent être ménagés sur le même capteur, comportant les mêmes protéines de liaison avec différents coefficients de liaison KD ou bien un réseau de protéines présentant une spécificité et/ou une affinité avec les ligands différente. L'analyse par un microprosseur de la réponse du IGFET à cette liaison permet, par exemple, de déterminer la concentration du constituant dans la solution. La mise en oeuvre d'une pluralité de protéines de liaison différentes permet de déterminer la concentration de différents constituants. De la même manière, l'emploi de protéines de liaison ayant des coefficients de liaison KD différents permet d'analyser le signal de sortie du capteur de façon numérique ou analogique, ou les deux combinées. Pour libérer le constituant de la protéine, on applique de la chaleur au capteur au moyen d'un assemblage à résistance intégré dans la puce dudit capteur. Dans une variante, on peut appliquer une polarisation inversée à la gâchette en vue d'accélérer cette libération.
PCT/US1988/001494 1987-05-05 1988-05-04 Systeme mettant en oeuvre un capteur biologique pour controler une caracteristique d'un constituant selectionne dans un milieu WO1988008972A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4708087A 1987-05-05 1987-05-05
US047,080 1987-05-05

Publications (1)

Publication Number Publication Date
WO1988008972A1 true WO1988008972A1 (fr) 1988-11-17

Family

ID=21946965

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1988/001494 WO1988008972A1 (fr) 1987-05-05 1988-05-04 Systeme mettant en oeuvre un capteur biologique pour controler une caracteristique d'un constituant selectionne dans un milieu

Country Status (3)

Country Link
EP (1) EP0313629A1 (fr)
CA (1) CA1318353C (fr)
WO (1) WO1988008972A1 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5328847A (en) * 1990-02-20 1994-07-12 Case George D Thin membrane sensor with biochemical switch
GB2287296A (en) * 1994-03-08 1995-09-13 Pektron Ltd Detecting passage of cleaning liquid in a beer line
EP0751392A2 (fr) * 1995-06-28 1997-01-02 Motorola, Inc. Biocapteur ou capteur chimique avec couche indicatrice
FR2747785A1 (fr) * 1996-04-17 1997-10-24 Motorola Inc Procede et appareil de detection moleculaire a base de transistor
FR2752616A1 (fr) * 1996-08-20 1998-02-27 Motorola Inc Appareil a base de transistor et procede de detection moleculaire et d'accroissement de l'intensite du champ
WO2004036203A1 (fr) * 2002-10-14 2004-04-29 Infineon Technologies Ag Systeme detecteur et mode de fonctionnement de ce dispositif detecteur

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3831432A (en) * 1972-09-05 1974-08-27 Texas Instruments Inc Environment monitoring device and system
FR2304083A1 (fr) * 1975-03-12 1976-10-08 Univ Utah Dispositif a effet de champ sensible a des produits chimiques, pour appareils detecteurs
US4238757A (en) * 1976-03-19 1980-12-09 General Electric Company Field effect transistor for detection of biological reactions
US4334880A (en) * 1980-10-20 1982-06-15 Malmros Mark K Analytical device having semiconductive polyacetylene element associated with analyte-binding substance
JPS59125054A (ja) * 1983-01-05 1984-07-19 Kuraray Co Ltd イオン濃度測定装置
WO1985004480A1 (fr) * 1984-03-22 1985-10-10 Stichting Centrum Voor Micro-Electronica Twente Procede de production d'un isfet, et isfet obtenu par ce procede
EP0214805A2 (fr) * 1985-08-29 1987-03-18 Matsushita Electric Industrial Co., Ltd. Capteur utilisant un transistor à effet de champ et méthode pour le fabriquer

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3831432A (en) * 1972-09-05 1974-08-27 Texas Instruments Inc Environment monitoring device and system
FR2304083A1 (fr) * 1975-03-12 1976-10-08 Univ Utah Dispositif a effet de champ sensible a des produits chimiques, pour appareils detecteurs
US4238757A (en) * 1976-03-19 1980-12-09 General Electric Company Field effect transistor for detection of biological reactions
US4334880A (en) * 1980-10-20 1982-06-15 Malmros Mark K Analytical device having semiconductive polyacetylene element associated with analyte-binding substance
JPS59125054A (ja) * 1983-01-05 1984-07-19 Kuraray Co Ltd イオン濃度測定装置
WO1985004480A1 (fr) * 1984-03-22 1985-10-10 Stichting Centrum Voor Micro-Electronica Twente Procede de production d'un isfet, et isfet obtenu par ce procede
EP0214805A2 (fr) * 1985-08-29 1987-03-18 Matsushita Electric Industrial Co., Ltd. Capteur utilisant un transistor à effet de champ et méthode pour le fabriquer

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Patent Abstracts of Japan, volume 8, no. 254 (P-315)(1691), 21. November 1984; & JP-A-59125054 (KURARAY K.K.) 19 July 1984 see abstract *
Sensors and Actuators, volume 9, 1986, Elsevier Sequoia, (NL), W.J. Lian et al.: "Flip-flop sensors: a new class of silicon sensors", pages 259-268 *

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5328847A (en) * 1990-02-20 1994-07-12 Case George D Thin membrane sensor with biochemical switch
GB2287296A (en) * 1994-03-08 1995-09-13 Pektron Ltd Detecting passage of cleaning liquid in a beer line
GB2287296B (en) * 1994-03-08 1997-08-20 Pektron Ltd Flow sensing apparatus
EP0751392A2 (fr) * 1995-06-28 1997-01-02 Motorola, Inc. Biocapteur ou capteur chimique avec couche indicatrice
EP0751392A3 (fr) * 1995-06-28 1997-11-19 Motorola, Inc. Biocapteur ou capteur chimique avec couche indicatrice
FR2747785A1 (fr) * 1996-04-17 1997-10-24 Motorola Inc Procede et appareil de detection moleculaire a base de transistor
FR2752616A1 (fr) * 1996-08-20 1998-02-27 Motorola Inc Appareil a base de transistor et procede de detection moleculaire et d'accroissement de l'intensite du champ
EP0858597A1 (fr) * 1996-08-20 1998-08-19 Motorola, Inc. Appareil et procede a transistors pour la detection moleculaire et l'amelioration du champ
EP0858597A4 (fr) * 1996-08-20 1999-03-10 Motorola Inc Appareil et procede a transistors pour la detection moleculaire et l'amelioration du champ
WO2004036203A1 (fr) * 2002-10-14 2004-04-29 Infineon Technologies Ag Systeme detecteur et mode de fonctionnement de ce dispositif detecteur
US7662341B2 (en) 2002-10-14 2010-02-16 Infineon Technologies Ag Sensor arrangement and method for operating a sensor arrangement

Also Published As

Publication number Publication date
EP0313629A1 (fr) 1989-05-03
CA1318353C (fr) 1993-05-25

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