WO1988006618A1 - Agencement pour la micropropagation de plantes - Google Patents

Agencement pour la micropropagation de plantes Download PDF

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Publication number
WO1988006618A1
WO1988006618A1 PCT/HU1987/000010 HU8700010W WO8806618A1 WO 1988006618 A1 WO1988006618 A1 WO 1988006618A1 HU 8700010 W HU8700010 W HU 8700010W WO 8806618 A1 WO8806618 A1 WO 8806618A1
Authority
WO
WIPO (PCT)
Prior art keywords
closing
cultivating
feeding
arrangement according
vessels
Prior art date
Application number
PCT/HU1987/000010
Other languages
English (en)
Inventor
Miklós FÁRI
Original Assignee
KONTAKTA Villamosszerelési Vállalat
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by KONTAKTA Villamosszerelési Vállalat filed Critical KONTAKTA Villamosszerelési Vállalat
Priority to DE3790915A priority Critical patent/DE3790915C2/de
Priority to DE873790915T priority patent/DE3790915T1/de
Priority to PCT/HU1987/000010 priority patent/WO1988006618A1/fr
Priority to JP62501744A priority patent/JPH01503035A/ja
Priority to EP87901607A priority patent/EP0304417A1/fr
Priority to NL8720126A priority patent/NL8720126A/nl
Publication of WO1988006618A1 publication Critical patent/WO1988006618A1/fr
Priority to GB8826044A priority patent/GB2209535A/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001—Culture apparatus for tissue culture

Definitions

  • the invention relates to an arrangement for micropropagation of plants including an aseptical space and sterilized cultivating vessels. It is known that the methods commonly defjned as belonging to the biotechnology stand in the forefront of the appl ied biological research works and of the related scientific investigations. The biotechnological methods are especially important in propagation of tissues and cellular elements of the plants, wherein the propagation is applied also for investigating the plant systems.
  • the common feature of the micropropagation methods for propagating tissues and cel lular elements is that they are carried out in sterile conditions, in steri lized cultivating vessels, in vitro, in order to clarify the biological aspects.
  • the micropropagation of plants includes the foIlowing steps:
  • the steps mentioned above require generally the same means and pieces of equipment as in the microbiology, i.e. glass vessels for receiving a culture-medium including a cultivating component and a solidifying component, said culture-medium being warmed up.
  • the glass vessels are closed by a cotton-wool cork, whereby the respiration of the cultures is ensured but the sporules can not enter the vessels.
  • the cotton-wool cork is usually closed by an aluminium foil.
  • the transplantation is carried out in inoculation boxes wherefrom the products of the operation carried out in sterile conditions are transported to the place of cultivation and incubation.
  • the glass vessels have been replaced in some institute by vessels made of light transparent plastics and the cotton-wooI applied for closing can be substituted by corks made of foamed plastics, covering elements made of plastics, parafiIm or by PVC foil.
  • the object of the invention is to provide a method whereby the efficiency of the micropropagation can be improved with fulfilling the streng biological purity requirements similarly to the known solutions.
  • the invention is based on the recognition that it is possible to realise an equipment of high efficiency for micropropagation of plants when the sequence of the traditional operations of micropropagation is changed in the way that after preparing the culture-medium the following operations are carried out in sterile conditions: sterilizing the medium, feeding the necessary components into the vessel, transplanting the plants and closing the vessel. The incubation does not require sterile conditions.
  • the object of the invention is therefore an arrangement for micropropagation of plants, including an aseptical space, sterilized cultivating vessels, a storing unit for receiving a feeding apparatus for forwarding the cultivating vessels, means e.g.
  • an ultraviolet lamp for ensuring sterility of the cultivating vessels in the storing unit
  • conveyor means arranged under the feeding apparatus or in the environment thereof, storage means for receiving sterilized breeding components, storing means for receiving sterilized solidifying components, at least one feeding head connected to the storage means and the storing means, a mixing head connected to the at least one feeding head including an outlet opening, thermostating means for ensuring required temperature of the storage means, and the storing means preferably of the at least one feeding head and the mixing head means for sterilized closing the cultivating vessels comprising a first group of a supporting element for receiving a closing foil arranged over the conveyor means, cylindr ⁇ c forwarding means for forwarding the closing foil, a heating filament lying near to the cylindric forwarding means and a heat treatment unit and or a second group of a storage unit for receiving closing covers, feeding unit for forwarding the closing covers, means for ensuring sterility of the closing covers and if desired closing means for fixing the closing covers on the cultivating vesseIs.
  • the arrangement proposed by the invention preferably applies conveyor means with clawed surface, a flexible tube element for connecting the mixing head to the at least one feeding head and a slide arranged under the feeding apparatus.
  • multidirectional guiding means arranged at the output of the conveyor means and further conveyor means coupled to the guiding means having preferably c I awe d s u r f ace .
  • Figure 1 shows the arrangement of the invention in side view
  • Figure 2 il lustrates the arrangement of the Figure 1 in top view.
  • the arrangement as proposed by the invention is a laboratory device realised with closed outer surface for ensuring an aseptical space. This outer surface can be created e.g. by a laminar box, a laminary tunnel or by a laminary chamber.
  • the arrangement as proposed makes use of steri lized cultivating vessels made of light transparent or not transpare plastics as poliethylen, poI ipropyIen, poI icarbonate etc. They are preferably rectangular boxes or vessels of other shapes which can be placed in one another.
  • the cultivating vessels are arranged in a storing unit 1 lyint in one another or in a reel.
  • a feeding apparatus 2 is connected to the output of the storing unit 1 ( Figure 1) wherein means 3 ensure sterility of the cultivating vessels.
  • the means 3 can be formed e.g. by an ultraviolet lamp.
  • a slide 4 connects the output of the feeding apparatus 2 with conveyor means 5 for transporting the cultivating vessels to further operation stages.
  • multidirectional guiding means 10 can be arranged at the end of the conveyor means 5 for selecting the cultivating vessels according to different directions of forwarding. In this case further conveyor means 11 are foreseen.
  • the cultivating are transported before the output of a mixing head 8 receiving steri lized breeding components and steri l ized sol idifying components from storage means 61 and storing means 62 respectively, over at least one feeding head 71, 72 ( Figure 2).
  • the storage means 61 and the storing means 62 are arranged in respective thermostat ing means 91, 92 ensuring their required temperature.
  • the cultivating vessels filled with the components of the culture-medium are forwarded to means for sterilized closing them. This is realised in an appropriate way, e.g.
  • closing covers arranged in a storage unit 15 equipped with means 17 for ensuring sterility of the closing covers and with feeding unit for forwarding the closing covers to the vessels to be closed.
  • the closing covers are pressed onto the cultivating vessels by closing means 18, if this is required.
  • the sterile connection between the cultivating vessel and the PVC foil is ensured by a heating unit 19 for blowing heat air.
  • the closing PVC foil is arranged on a supporting element 12.
  • the foil can be made of other appropriate materials, if required.
  • the next step is to place the cultivating vessels in the storing unit 1 and to connect the feeding head 71 with the storage means 61 comprising the sterilized breeding components.
  • the sterilized solidifying components comprised in the storing means 62 is be warmed up to the liquid state and then in the aseptical space the feeding head 72 can be connected to the storing means 62.
  • the feeding heads 71, 72 should be coupled with the mixing head 8, e.g. by a flexible tube element.
  • the storage units prepared in this way can be placed in the thermostating means 91, 92 ensuring their required temperature.
  • the supporting means 12 are, if necessary, equipped with rol led PVC foil, the free end of the foil has to be fixed on the surface of the cyl indric forwarding means 13, in the near environment of the heating filament 14. If applied, the storage unit 15 should be fil led up with closing covers made of appropriate plastics, lying over one another. The arrangement is connected thereafter to a control unit for regulating the operation conditions.
  • the operations are the fol lowing:
  • the feeding apparatus 2 lets a cultivating vessel move to the output opening of the mixing head 8. In this operation the si ide 4 can be helpful.
  • the mixing head 8 receives the necessary components in required amount from the storage unit 61 and storage means 62 over the feeding heads 71, 72.
  • the solidifying component makes the culture-medium solidify.
  • the conveyor means 5 transport the cultivating vessels to the multidirectional guiding means 10, where the transplantation can be carried out.
  • the further conveyor means 11 transport the cultivating vessel to the fol lowing operations.
  • transplantation can be carried out in the cultivating vessel during transporting it on the surface of the conveyor means 5, making use of a short stop thereof.
  • the multidirectional guiding means 10 are not necessary.
  • the conveyor means 5 or 11 forward the cultivating vessels to the means for steril ized closing them.
  • the cultivating vessel stops underneath the cyl indric forwarding means 13 wherefrom the PVC foil is pressed to the vessel.
  • the heating filament 14 cuts the foi l and in a heat treatment unit 19 under the influence of relatively high temperature the foi I is tensioned on the upper part of the cultivating vessel. In this way the sterile conditions are fully realised.
  • Another preferred solution is to apply closing covers made of plastics.
  • the feeding unit 16 forwards the covers to the upper surface of the vessel where they are pressed to the vessel by the closing means 18.
  • the cultivating vessels closed in the aseptic space are taken away from the inner parts of the arrangement, wherein the closing means applying plastic covers and foil can be applied also simultaneously.
  • the closing covers are made in this case with perforation or other lacks of material as requied in the following operational steps.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

L'agencement décrit, qui sert à la micropropagation de plantes comportant un espace aseptique et des récipients de culture stérilisés, comprend dans l'espace aseptique une unité de stockage (1) destinée à recevoir un appareil d'avance (2) servant à faire avancer les récipients de culture, un organe (3) destiné à assurer la stérilité des récipients de culture (1) dans l'unité de stockage, un organe de transport (5) disposé sous l'appareil d'avance (2) ou dans son voisinage, un organe de stockage (61) destiné à recevoir des composants de croissance stérilisés, un organe de stockage (62) destiné à recevoir des composants de solidification stérilisés, au moins une tête d'alimentation (71, 72) reliée à l'organe de stockage (61) et l'organe de stockage (62), une tête de mélange (8) reliée à ladite tête d'alimentation (71, 72) comportant une ouverture de sortie, un organe de commande thermostatique (91, 92) servant à assurer la température requise dans l'organe de stockage (61) et l'organe de stockage (62) et un organe pour la fermeture stérile des récipients de culture. L'agencement proposé peut être utilisé pour effectuer des opérations biotechnologiques sur des plantes dans des laboratoires industriels et scientifiques spécialisés.
PCT/HU1987/000010 1987-03-06 1987-03-06 Agencement pour la micropropagation de plantes WO1988006618A1 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DE3790915A DE3790915C2 (fr) 1987-03-06 1987-03-06
DE873790915T DE3790915T1 (de) 1987-03-06 1987-03-06 Anordnung zur mikrovermehrung von pflanzen
PCT/HU1987/000010 WO1988006618A1 (fr) 1987-03-06 1987-03-06 Agencement pour la micropropagation de plantes
JP62501744A JPH01503035A (ja) 1987-03-06 1987-03-06 植物の微量増殖用装置
EP87901607A EP0304417A1 (fr) 1987-03-06 1987-03-06 Agencement pour la micropropagation de plantes
NL8720126A NL8720126A (nl) 1987-03-06 1987-03-06 Inrichting voor het microvoortplanten van planten.
GB8826044A GB2209535A (en) 1987-03-06 1988-11-07 Arrangement for micropropagation of plants

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/HU1987/000010 WO1988006618A1 (fr) 1987-03-06 1987-03-06 Agencement pour la micropropagation de plantes

Publications (1)

Publication Number Publication Date
WO1988006618A1 true WO1988006618A1 (fr) 1988-09-07

Family

ID=10980753

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/HU1987/000010 WO1988006618A1 (fr) 1987-03-06 1987-03-06 Agencement pour la micropropagation de plantes

Country Status (6)

Country Link
EP (1) EP0304417A1 (fr)
JP (1) JPH01503035A (fr)
DE (2) DE3790915C2 (fr)
GB (1) GB2209535A (fr)
NL (1) NL8720126A (fr)
WO (1) WO1988006618A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990010381A1 (fr) * 1989-03-07 1990-09-20 Plant Production Systems B.V. Procede de production de bacs de culture remplis de microcultures de vegetaux, et dispositif de mise en ×uvre dudit procede
WO1991015110A1 (fr) * 1990-04-05 1991-10-17 British Technology Group Plc Procedes et appareil de micropropagation
EP0468381A1 (fr) * 1990-07-23 1992-01-29 Kirin Beer Kabushiki Kaisha Appareil pour la coupe et la transplantation de tissus de plantes
WO1993019586A1 (fr) * 1992-03-27 1993-10-14 Microcrop (Ireland) Limited Procede et appareil de micropropagation ameliores

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0132414A2 (fr) * 1983-07-26 1985-01-30 P.B. Ind. Plant Biotech Industries Ltd. Procédé de propagation de cultures de tissus de plantes
WO1986004919A1 (fr) * 1985-02-14 1986-08-28 Agrogen-Stiftung Procede et dispositif pour la culture in vitro de cellules ou d'organes vegetaux, ainsi que pour la micropropagation et la regeneration de vegetaux entiers

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0132414A2 (fr) * 1983-07-26 1985-01-30 P.B. Ind. Plant Biotech Industries Ltd. Procédé de propagation de cultures de tissus de plantes
WO1986004919A1 (fr) * 1985-02-14 1986-08-28 Agrogen-Stiftung Procede et dispositif pour la culture in vitro de cellules ou d'organes vegetaux, ainsi que pour la micropropagation et la regeneration de vegetaux entiers

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990010381A1 (fr) * 1989-03-07 1990-09-20 Plant Production Systems B.V. Procede de production de bacs de culture remplis de microcultures de vegetaux, et dispositif de mise en ×uvre dudit procede
EP0389019A1 (fr) * 1989-03-07 1990-09-26 Plant Production Systems B.V. Procédé de production de boîtes de culture remplies de microcultures de parties de plantes et appareil pour ce procédé
WO1991015110A1 (fr) * 1990-04-05 1991-10-17 British Technology Group Plc Procedes et appareil de micropropagation
EP0468381A1 (fr) * 1990-07-23 1992-01-29 Kirin Beer Kabushiki Kaisha Appareil pour la coupe et la transplantation de tissus de plantes
US5225345A (en) * 1990-07-23 1993-07-06 Kirin Beer Kabushiki Kaisha Plant tissue cutting and transplanting apparatus
WO1993019586A1 (fr) * 1992-03-27 1993-10-14 Microcrop (Ireland) Limited Procede et appareil de micropropagation ameliores
GB2272824A (en) * 1992-03-27 1994-06-01 Microcrop Improved micropropagation apparatus and method
GB2272824B (en) * 1992-03-27 1996-11-13 Microcrop Improved micropropagation apparatus and method

Also Published As

Publication number Publication date
DE3790915C2 (fr) 1990-10-11
JPH01503035A (ja) 1989-10-19
GB2209535A (en) 1989-05-17
DE3790915T1 (de) 1989-06-15
NL8720126A (nl) 1989-02-01
EP0304417A1 (fr) 1989-03-01

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