WO1987004465A1 - Procede pour la fermentation de matiere contenant de l'amidon - Google Patents

Procede pour la fermentation de matiere contenant de l'amidon Download PDF

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Publication number
WO1987004465A1
WO1987004465A1 PCT/SE1987/000023 SE8700023W WO8704465A1 WO 1987004465 A1 WO1987004465 A1 WO 1987004465A1 SE 8700023 W SE8700023 W SE 8700023W WO 8704465 A1 WO8704465 A1 WO 8704465A1
Authority
WO
WIPO (PCT)
Prior art keywords
fermentation
starch
ethanol
process according
amyloglucosidase
Prior art date
Application number
PCT/SE1987/000023
Other languages
English (en)
Inventor
Ingemar Bjurenvall
Ernst Conrad
Original Assignee
Ingemar Bjurenvall
Ernst Conrad
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ingemar Bjurenvall, Ernst Conrad filed Critical Ingemar Bjurenvall
Publication of WO1987004465A1 publication Critical patent/WO1987004465A1/fr
Priority to DK489687A priority Critical patent/DK489687D0/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Definitions

  • the present invention relates to a process for the fermenta ⁇ tion of starch containing material derived from cereals for the production of alcohols, preferably ethanol.
  • the object of the present invention is to obatin a possibility to saccharify and ferment starch from cereals in a simple and rational way to alcohols, preferably ethanol, with a simulta ⁇ neous high yield of alcohols, preferably ethanol.
  • the starch contents of the cereal grains are primarily saccharified, af ⁇ ter an elimination of bran and gluten, to yield a high dex ⁇ trose equivalent, typically 95-97 DE; previously by an acid hydrolysis of the starch contents, today by an enzymatic hyd ⁇ rolysis by using amyloglucosidase of a gelatinized starch hav- ing been presaccharified by the action of amylase, whereupon the completely saccharified starch is fermented by adding a suitable yeast culture, to yield ethanol at 37 C during 48 hrs .
  • a 100% yield of ethanol per 100 kg of starch shall be 57 kg of etha- nol according to the formulae:
  • pH is thereby generally 4.5.
  • Example 1 800 g of crushed wheat (15.5% H 2 0) were added to 3000 ml of water as well as 0.5 g of Q £-amylase (N0V0 Thermamyl). This slurry was heated to opti ma I o6-am lase hydrolysis to 95 C to obtain ge lat i ni zat on and presacchari fi cati on to a DE-value of 10-12, whereupon the slurry was subject to autoc lavat i on at 130 C to provide enzyme deactivation. The slurry was then cooled to 35 C and transferred to a fermentation flask. pH was hereby 6.5.
  • the mash obtained was then distilled to obtain a crude ethan ⁇ ol, comprising 33% per volume of ethanol.
  • the direct yield was 1000 mis of 33% ethanol, which means 412 mis of 100% ethanol per kg of wheat.
  • Example B (Comparative). 8 80000 gg ooff ccrruusshheedd wwhheeaatt ((15.5% H 2 0) and 0.5 g of -amylase (N0V0 Thermamyl) were added to 3000 ml of water. The slurry was heated 95°C and was then autoclaved at 130 C for enzyme deactivation, i.e., all n accordance with Example 1 above. pH was then adjusted to 4.5 from 6.5 by adding HCl.
  • the slurry was then cooled to 35 C and 50 g of yeast were add ⁇ ed and was allowed to ferment in a heating cabinet at 35 C for 24 hrs, when the C0_-release had ceased.
  • the mash obtained was then distilled which yielded 1000 ml of a distillate comprising 26.4% by volume of ethanol. The yield was thus 330 mis of 100% ethanol per kg of wheat.
  • Example 2 As evident from above a better yield was obtained using the process of the present invention, Example 1, than using a con ⁇ ventional 2-stage ethanol production.
  • Example B Example B.
  • amyloglucosidase can be reduced considerably in the present process, as it is only deactivated to a small extent at 35 C .
  • amyloglucosidase per kg crushed wheat, however, more preferably 1.0 g of amyloglucosi ⁇ dase per kg.
  • the whole contents of the grain can be present during the whole process, including the distilla ⁇ tion, whereby the distillate residue of bran and gluten can be used as a foodstuff or feedstuff additive after having been washed and dried.
  • the present invention has been described above with reference to the use of wheat, but of course, any other cereal can be used, as rye, oat, barley, ryewheat, maize, rice, sorghum, and others.
  • the total yield of ethanol is thereby dependent on the starch contents but the high yield compared with con ⁇ ventional 2-stage process is obtained in all cases. However, it seems to be less pronounced when using rice and maize.

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

Procédé permettant la fermentation de matières contenant de l'amidon et dérivés des céréales dans le but de produire des alcools, de préférence de l'éthanol, et dans lequel on pré-saccharifie, à l'aide d'une alpha-amylase, un équivalent dextrose de faible valeur, après quoi on ajoute simultanément une amyloglucosidase et une culture de levure appropriée pour la fermentation de l'éthanol puis on effectue une hydrolyse et une fermentation simultanées pour obtenir de l'éthanol à 35-37°C.
PCT/SE1987/000023 1986-01-21 1987-01-20 Procede pour la fermentation de matiere contenant de l'amidon WO1987004465A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DK489687A DK489687D0 (da) 1986-01-21 1987-09-17 Fremgangsmaade til gaering af stivelsesmatriale

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE8600253A SE453922B (sv) 1986-01-21 1986-01-21 Forfarande for jesning av sterkelsematerial
SE8600253-2 1986-01-21

Publications (1)

Publication Number Publication Date
WO1987004465A1 true WO1987004465A1 (fr) 1987-07-30

Family

ID=20363166

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SE1987/000023 WO1987004465A1 (fr) 1986-01-21 1987-01-20 Procede pour la fermentation de matiere contenant de l'amidon

Country Status (5)

Country Link
EP (1) EP0257045A1 (fr)
AU (1) AU6899687A (fr)
DK (1) DK489687D0 (fr)
SE (1) SE453922B (fr)
WO (1) WO1987004465A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2630751A1 (fr) * 1988-05-02 1989-11-03 Rhone Poulenc Chimie Procede de production d'acide itaconique
EP0443844A1 (fr) * 1990-02-20 1991-08-28 A.E. Staley Manufacturing Company Hydrolysats d'amidon
RU2495926C2 (ru) * 2006-03-17 2013-10-20 Этаблиссман Ж. Суффле Питательная добавка для среды спиртового брожения
WO2014197705A1 (fr) * 2013-06-07 2014-12-11 Novozymes A/S Procédé et compositions permettant d'améliorer un polypeptide x143 ayant une activité de dissolution d'amidon rétrogradé et leurs utilisations

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4316956A (en) * 1980-02-06 1982-02-23 Novo Industri A/S Fermentation process

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4316956A (en) * 1980-02-06 1982-02-23 Novo Industri A/S Fermentation process

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, Vol 102, 1985 No 130414y, Rev Microbiol 1984, 15 (4), 226-71 (Port) *
CHEMICAL ABSTRACTS, Vol 103, 1985 No 107569q, Biomass 1985, 7 (3), 171-83 (Eng) *
CHEMICAL ABSTRACTS, Vol 103, 1985 No 159081n, Sanop Misaengmul Hakhoechi 1984, 12 (4), 261-4 (Korean) *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2630751A1 (fr) * 1988-05-02 1989-11-03 Rhone Poulenc Chimie Procede de production d'acide itaconique
EP0341112A1 (fr) * 1988-05-02 1989-11-08 Rhone-Poulenc Chimie Procédé de production d'acide itaconique
EP0443844A1 (fr) * 1990-02-20 1991-08-28 A.E. Staley Manufacturing Company Hydrolysats d'amidon
RU2495926C2 (ru) * 2006-03-17 2013-10-20 Этаблиссман Ж. Суффле Питательная добавка для среды спиртового брожения
WO2014197705A1 (fr) * 2013-06-07 2014-12-11 Novozymes A/S Procédé et compositions permettant d'améliorer un polypeptide x143 ayant une activité de dissolution d'amidon rétrogradé et leurs utilisations

Also Published As

Publication number Publication date
SE8600253D0 (sv) 1986-01-21
DK489687A (da) 1987-09-17
EP0257045A1 (fr) 1988-03-02
SE8600253L (sv) 1987-07-22
DK489687D0 (da) 1987-09-17
SE453922B (sv) 1988-03-14
AU6899687A (en) 1987-08-14

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