Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
  • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
  • A61L33/00—Antithrombogenic treatment of surgical articles, e.g. sutures, catheters, prostheses, or of articles for the manipulation or conditioning of blood; Materials for such treatment
  • A61L33/0005—Use of materials characterised by their function or physical properties
  • A61L33/0011—Anticoagulant, e.g. heparin, platelet aggregation inhibitor, fibrinolytic agent, other than enzymes, attached to the substrate
• • C—CHEMISTRY; METALLURGY
  • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
  • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
  • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
  • C08L5/10—Heparin; Derivatives thereof
• • D—TEXTILES; PAPER
  • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
  • D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
  • D01F9/00—Artificial filaments or the like of other substances; Manufacture thereof; Apparatus specially adapted for the manufacture of carbon filaments
• • D—TEXTILES; PAPER
  • D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
  • D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
  • D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
  • D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
  • D04H1/54—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties by welding together the fibres, e.g. by partially melting or dissolving
  • D04H1/56—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties by welding together the fibres, e.g. by partially melting or dissolving in association with fibre formation, e.g. immediately following extrusion of staple fibres

Definitions

• the heparinizing of blood especially for use in the in vitro testing of blood samples.
• Heparin is normally provided by the pharmaceu ⁇ tical industry as an alkali metal (for example, primarily sodium) or alkaline earth (for example calcium) salt in view of the limted stability of the free acid form of heparin.
• the salts are most commonly provided for pharmaceutical use in the form of solutions. Solid heparin salts tend to be somewhat hygroscopic and grad ⁇ ually absorb water unless maintained in a low humidity environment. They are amorphous rather than crystalline and are available as fine powders.
• heparin salts are conventionally utilized in blood-gas analyses wherein they serve as anticoagulants to main- tain liquidity of the blood being tested.
• Blood-gas analyses are widely used in diagnostic medicine, oxygen and carbon dioxide contents of blood samples being of particular importance. From such measurements, a physician may obtain accurate oxygen level readings from which he may more accurately anticipate the patient's supplementary oxygen needs.
• the measurement of arterial blood gas normally involves drawing a sample of blood into a syringe con ⁇ taining an anticoagulant and then injecting the blood sample into an analyzing instrument.
• the anticoagulant is used to maintain the liquidity of the blood sample so that the partial pressures of the blood gases are at substantially the same level as when initially drawn. Even though the procedure seems simple and straightforward there are numerous opportunities for sources of error to be introduced. One particular source of error has been found to result from the method in which the anticoagulant is added to the drawn blood sample.
• the first method involves the drawing of a quantity of heparin solution into a syringe in order to wet the interior walls.
• a substantial portion of the excess heparin is ejected and the blood is then drawn into the syringe from the patient.
• the blood when drawn mixes with the heparin solution to prevent coagulation.
• the error in this method results from the fact that an indeterminate amount of heparin solution remains in the syringe interior, needle hub and cannula.
• the drawn blood sample is diluted by an indeterminate amount of heparin solution which in most cases leads to less accurate blood gas data.
• a second method involves the use of preheparin- ized syringes.
• These syringes are prepared by depositing a lyophilized heparin on the internal surface of the syringe. They have been found to vary in the location and the amount of heparin on the syringe barrel wall. Also, the deposited heparin dissolution rate into the blood is slower than optimal and is unpredictable. This slow dissolution rate combined with the variability in amount allows partial blood coagulation thereby introducing a source of error into the analysis. Because lyophilized heparin is more difficult and complex to manufacture and use than a heparin solution, these preheparinized syringes have been found to be much more costly without proportionately minimizing the amount of potential error.
• a third method recently introduced comprises placing an anticoagulant tablet in the hub of the needle of the syringe used to obtain a blood sample from a patient.
• the blood flowing through the needle and into the syringe dissolves the tablet and the blood is heparinized.
• These tablets are comprised of a salt of heparin, a tablet binder and a pH controlling substanc
• the present invention relates to microfibers of amorphous heparin salts which have average length to di ⁇ ameter ratios of at least 20 (and preferably at least 80) , to open, nonwoven webs of the fibers, to processes for their preparation and to a method for heparinizing blood which comprises intermixing heparin salt microfibers (ordinarily a web of such fibers) with blood.
• the microfibers are of particular use in in vitro blood test procedures. They provide a much more rapidly dissolving heparin source which does not dilute the blood which is heparinized. They are easily and inexpensively prepared and can be conveniently quantized in individual test needles and syringes.
• the process of the invention comprises expressing an aqueous heparin salt solution into a large excess of a fluid which is a nonsolvent for the heparin salt.
• a fluid nonsolvent a gas stream such as air or nitrogen or a liquid nonsolvent such as isopropanol
• the water can be removed very rapidly from the heparin salt solution while the latter is in rapid laminar motion and the heparin salt fibers are formed, rather than a blocky precipitate.
• the ratio (by volume) of the nonsolvent to the aqueous heparin salt solution is 10 or greater (e.g. from 10 to 50) and is preferably 20 or more.
• FIGURE 1 is a schematic diagram of an apparatus for preparing a microfibrous web of the present invention
• FIGURE 2 is an enlarged section through a micro fibrous web of the present invention.
• FIGURE 3 is a schematic diagram illustrative of an alternative apparatus for the preparation of a icro- fibrous web of the present invention.
• the microfibers are formed in a stream of an inert gas.
• the icrofiber- blowing portion of the apparatus can be a conventional structure as taught, for example, in Wente, Van A., "Superfine Thermoplastic Fibers," in Industrial Engineerin Chemistry, Vol. 48, pages 1342 et seq (1956), or in Report No. 4364 of the Naval Research Laboratories, published May 25, 1954, entitled “Manufacture of Superfine Organic Fibers" by Wente, V. A.; Boone, D. C. ; and Fluharty, E. L.
• Such a structure includes a die 10 which has a solution injection chamber 11 through which liquified fiber-forming solution is advanced; die orifices 12 arranged in line across the forward end of the die and through which the fiber-forming solution is directed; and cooperating gas orifices 13 through which a gas, typically air or nitrogen, is forced at very high velocity.
• the high-velocity gaseous stream draws out and attenuates the extruded fiber-forming heparin solution, whereupon the fiber-forming heparin solidifies as microfibers during travel to a collector 14.
• the collector 14 is typically a finely perforated screen, which in this case is in the form of a closed-loop belt, but which can take alternative forms, such as a flat screen or a drum or cylinder.
• Gas- withdrawal apparatus may be positioned behind the screen to assist in deposition of the microfibrous web 22 and removal of gas. It will be understood by the art that operating conditions must be controlled to avoid der composition of the heparin. ⁇ * - ⁇ - ⁇ - % r
• the web as shown in FIG. 2, can be cut or shaped into a variety of forms, e.g. plugs, discs, cubes, etc.
• the crossover points of the individual fibers in the webs may be partly or completely fused.
• the apparatus shown in FIG. 3 forms the webs by precipitating the microfibers from solution.
• the apparatus includes a heparin solution injecting portion, a nonsolvent fluid circulating system and a web take-off portion.
• the fluid system is comprised of a collection reservoir 30 and a filament attenuation section 36. In line between the reservoir 30 and attenuation section 36 is a pump 32 and a flow meter 34 which may be used to control the flow rate of the fluid.
• the selection of the fluid is dependent on the environment in which the heparin is to be used.
• the heparin solution is injected into the fluid path at filament attenuation section 36 through injection orifice 44.
• the heparin solution enters injection orifice 44 from the heparin reservoir 38 after passing through control valve 40 and flow meter 42.
• the movement of the heparin solution may be facilitated by numerous means known to the art, e.g. a pump, air pressure, etc.
• the combined streams e.g.
• the fluid and heparin are directed to the web take-off portion which is comprised of a movable collector 48 where the microfibers are collected in web form and the fluid returns to the fluid reservoir 30 for recycling.
• Screen 48 move ⁇ ment is facilitated by rollers 51 and 53 and motor 52.
• the width of the formed web 54 is controlled by the gauge bars 46.
• the web 54 passes under a wringer roller 50 which further aids in the removal of the fluid from web 54.
• Web 54 may then be directed to a drier or to further processing as desired.
• heparin salt webs of the invention are, as noted previously, particularly useful in and adapted for
• OMPI certain in vitro blood tests, e.g. in which the blood is subjected to them immediately upon being drawn from a mammalian subject in order to stabilize the blood in a liquid state suitable for testing. This stabilization reaction is commonly referred to as heparinization.
• the webs are, when properly packaged and stored stable for years under ambient conditions or longer when stored cold. At relative humidities of approximately 55 percent (at ambient temperatures, e.g. 20-25° C) they are still stable for several months although under very high humidity conditions they absorb an excess of moisture and deform grossly. They dissolve very rapidly in aqueous solutions, .including blood, due, it appears, to the open structure and relatively high surface area of the micro- fibers and to the absence of any solid excipient or other additive. Thus, relatively small pledgets of heparin web (less than about one cubic centimeter) have been found to dissolve completely in about one second in water or blood. The webs are moderately stable to cobalt irradi tion and can thus be easily sterilized.
• the heparin salt microfibers are capable of absorbing up to about three megarads while retaining about 83 percent of the USP activity and 77 percent of the anti Factor X activity of the heparin salt starting material.
• the density of the individual microfibers is about 1.8 g/cc.
• the acidity of solutions of the heparin salt microfibers depends upon the acidity of the solution from which they were originally perpared and the acidity of the water used to dissolve them. However, the webs do not significantly affect the pH of the blood samples whic are heparinized due to the relatively small amount used and the buffering capacity of blood.
• the length and diameter of the microfibers is dependent upon the process used to prepare them, for example stirring rate, means of extrusion, velocity of extrusion, the nonsolvent (if any) used and the ratio of nonsolvent to water used.
• stirring rate means of extrusion
• velocity of extrusion means of extrusion
• nonsolvent if any
• ratio of nonsolvent to water used the ratio of nonsolvent to water used.
• shorter fibers are less useful for many purposes because they are difficult to handle and form poorer webs and mats.
• the average length to diameter ratio of the fibers is preferably at least 20 and is more preferably at least about 80.
• the strength and structural integrity of the webs render them ideally suited for rapidly heparinizing blood under controlled conditions , e.g. in the blood gas analysis test.
• Such pieces of the web may be placed in a syringe in any acceptable way, but are preferably inserted into the hub of a needle through which blood to be analyzed is drawn.
• the heparin web pieces heparinize the blood drawn through the needle virtually instantaneously and essentially eliminate all presently known sources of error due to inadequate heparinization during blood gas analysis. This method is faster than any previous heparinization method, and allows immediate anaylsis of the blood sample drawn.
• the weight of the fragment to be used in a single syringe depends upon the specific USP activity of the heparin and will be gauged to provide a desired level of USP activity, for example at least 250 USP units of anticoagulant activity.
• the density of the web and the size of the cut piece may be varied as desired.
• Typical sizes are 1.5 to 4.0 mm. in diameter and 1 to 5 mm. in thickness. Thinner discs of larger diameter of heparin web of at least 250 units activity may be placed in the barrel of the syringe before inserting the plunger as an alternative to placing the web in the needle hub.
• the process of preparing the microfiber comprises expressing an aqueous heparin salt solution into a large excess of a fluid which is a nonsolvent for the heparin salt.
• the fluid can be gas which, is inert with respect to the heparin salt, such as nitrogen or air or a liquid nonsolvent for heparin salt and the process can be carried out as a batch operation or continuously.
• An aqueous solution of from about 40 percent to 60 percent heparin salt by weight is ex- presed through a small concentric orifice into a stream of dry inert gas and blown onto a collection screen. See FIG. 1.
• heparin salt by weight is expressed into a rapidly stirred dry liquid nonsolvent for heparin such as methanol, ethano
• An aqueous solution of from 10 percent to 30 percent by weight heparin salt is continuously mixed into a stream of nonsolvent liquid and the microfibers are collected on a moving screen while the nonsolvent is recirculated and excess water is removed from the solvent stream.
• the concentration of nonsolvent is maintained at 95 percent or higher. See FIG. 3.
• the concentration of the nonsolvent must be retained at greater than 90 percent by volume at all times during the process (the remainder of less than 10 percent being the aqueous solution of the heparin salt) and preferably the concentration of the liquid nonsolvent is retained at 95 percent or even better, 99 percent or more by volume.
• the concentration of the liquid nonsolvent is maintained at 90 percent of nonsolvent liquid.
• microfibers increase in length (and hence in length to diameter ratio) as the concentration of the water decreases.
• the preferred liquid nonsolvent is isopropanol since it produces the best microfibers.
• microfibers obtained from the foregoing pro ⁇ cannot contain some water and, where a liquid nonsolvent has been used, normally some of it as well.
• .- nonsolvent and water content of the microfibers generally ranges up to 25 percent, usually 10 to 15 percent.
• the webs are dried by conventional methods such as vacuum oven, streams of dry gas, expressing or cen- trifuging off excess fluid followed by oven or gas drying, and the like to maintain flexibility and pliability.
• the resulting webs may then be cut, sliced, punched or divided in other conventional ways due to the inherent strength and structural integrity of the webs. More specifically, a web of the microfibers may be dried in a vacuum oven for example at about 60°C and will ordinarily reach desirable handling characteristics after 2 to 3 hours, Such a drying cycle typically produces product having a USP LOD (loss on drying) of about 7 to 11 percent.
• heparin salt microfibers When heparin salt microfibers are overdried, they become flaky and fragile but will absorb moisture under controlled humidity, e.g. about 25 to 35 percent, preferably 30 to 35 percent, from the atmosphere and again become pliable.
• Example 1 A sample of 10 g. of sodium haparin (U.S.P. activity 161 units per milligram) is dissolved in 15 g. of water to provide a solution of 40 percent by weight of sodium heparin.
• the solution is passed through a hypodermic needle with a tip opening of 0.89 mm. (18 gauge needle) by using a syringe as the pump.
• the solution expressed from the needle is blown by a stream of co - pressed air at a pressure of about 34000 N/m 2 • blown through a 6.35 mm. diameter nozzle.
• the resulting micro ⁇ fibers are further attenuated and dried by blowing a stream of warm air from a heat gun over the stream issuing from the nozzle.
• the sodium heparin microfibers formed are
• OMPI_ WIPO 2 collected at a web screen consisting of a 232 cm piece of standard laboratory burner gauge. 300 Mg. of the microfibers are obtained from 3 ml. of the solution (a yield of 25 percent) , and the balance of the sodium heparin is collected as droplets in a pan.
• a portion of the resulting web is found to dissolve instantly in water.
• Example 2 The web of material from Example 1 is evaluated as follows :
• a 12 mm. diameter disc of the web weighing abou 2 mg. is dropped into a small (about 0.5 ml.) quantity of rabbit blood on a watch glass and observed to dissolve immediately.
• Three 13 mm. diameter discs of the web are cut and weighed by difference after being inserted into the barrel of 5 ml. plastic syringes.
• Human blood (1 ml.) is drawn into each of the syringes from a reference supply o blood.
• the web dissolves in less than one second. No clotting is observed.
• the pH of both the reference suppl of blood and each of the heparinized samples is checked as shown in Table I below by using the pH sensor in a commercial blood gas analyzer.
• the sodium heparin web does not significantly affec the pH of the blood sample.
• Example 3 0.8 Ml. of a 25 percent by weight aqueous solution of sodium heparin is expressed into a stirred reactor containing 50 ml. of isopropanol from a syringe through a 22 gauge needle. The .resulting microfibers collect and mat around the stirring bar. The mat or web
• C P is pressed between two pieces of filter paper, placed on a vacuum filter apparatus covered with a rubber dam and dried by pulling a vacuum on the apparatus.
• Example 4 A syringe with a 22 gauge needle is used to ex ⁇ trude 6.2 g. of a 22.5 aqueous solution of sodium heparin into a magnetically stirred reactor containing 120 ml. of anhydrous isopropanol. The mixture is poured into a ho o- genizer and homogenized for about 2 minutes at high speed. The mixture is transferred to the Buchner funnel of a vacuum filter apparatus, allowed to settle and a vacuum is applied. A piece of filter paper is placed on top of the sodium heparin web or mat, and a rubber dam is used over the Buchner funnel. After the web has been pulled dry under vacuum, it is placed in a vacuum drying over at 85°C for about 18 hours. The weight of the web is 1.587 g. (1.395 g. theoretical) indicating about 15 per- cent solvent content.
• the web is cut with a 3.8 mm. cork borer to form discs of the heparin web weighing about 2.5 mg. which dissolve very rapidly in blood or water.
• Example 5 A sample of heparin web is prepared using the method of Example 4. The web is photomicrographed and the dimensions of fibers of the web are measured. The diameter of the fibers range from 3 to 50 microns. The relative lengths of the individual fibers are measured to be at least 20 to 30 times greater than the diameter.
• the lengths of the fibers are 80 or more times greater than the diameter.

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• Health & Medical Sciences (AREA)
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• Textile Engineering (AREA)
• Medicinal Chemistry (AREA)
• Epidemiology (AREA)
• Animal Behavior & Ethology (AREA)
• General Health & Medical Sciences (AREA)
• Public Health (AREA)
• Veterinary Medicine (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Polymers & Plastics (AREA)
• General Chemical & Material Sciences (AREA)
• Pharmacology & Pharmacy (AREA)
• Molecular Biology (AREA)
• Materials Engineering (AREA)
• Hematology (AREA)
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• Materials For Medical Uses (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
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Citations (8)

• 1981
  • 1981-04-20 JP JP56501940A patent/JPS57500982A/ja active Pending
  • 1981-04-20 WO PCT/US1981/000522 patent/WO1981003277A1/en not_active Ceased
  • 1981-04-20 AU AU72218/81A patent/AU7221881A/en not_active Abandoned
  • 1981-04-24 EP EP81301825A patent/EP0041771A3/en not_active Ceased
  • 1981-05-06 CA CA000376962A patent/CA1156650A/en not_active Expired
  • 1981-05-22 ZA ZA00813468A patent/ZA813468B/xx unknown
• 1982
  • 1982-01-15 NO NO820122A patent/NO820122L/no unknown
  • 1982-01-22 DK DK27282A patent/DK27282A/da not_active Application Discontinuation

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