UY39196A - MODIFIED BACTERIAL STRAINS TO IMPROVE NITROGEN FIXATION - Google Patents

MODIFIED BACTERIAL STRAINS TO IMPROVE NITROGEN FIXATION

Info

Publication number
UY39196A
UY39196A UY0001039196A UY39196A UY39196A UY 39196 A UY39196 A UY 39196A UY 0001039196 A UY0001039196 A UY 0001039196A UY 39196 A UY39196 A UY 39196A UY 39196 A UY39196 A UY 39196A
Authority
UY
Uruguay
Prior art keywords
nitrogen
nitrogen fixation
gene
bacterial strains
modified bacterial
Prior art date
Application number
UY0001039196A
Other languages
Spanish (es)
Inventor
Karsten Temme
Neal Shah
Bilge Ozaydin Eskiyenenturk
Sarah Bloch
Alvin Tamsir
Original Assignee
Pivot Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2020/031201 external-priority patent/WO2021221690A1/en
Priority claimed from PCT/US2021/013120 external-priority patent/WO2021146209A1/en
Application filed by Pivot Bio Inc filed Critical Pivot Bio Inc
Publication of UY39196A publication Critical patent/UY39196A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/26—Klebsiella (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/22—Klebsiella

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se proporcionan métodos y sistemas para generar y utilizar una bacteria diseñada genéticamente que comprende una modificación en un gen que regula la fijación o asimilación de nitrógeno, donde la modificación en el gen que regula la fijación o asimilación de nitrógeno da como resultado una o más de las siguientes: expresión constitutiva de un gen nifA en condiciones limitantes y no limitantes de nitrógeno, actividad de nifA en condiciones no limitantes de nitrógeno, disminución de la actividad uridililtransferasa de GlnD, disminución de la actividad de eliminación de adenililo de GlnE, y aumento de la excreción de amonio.Methods and systems are provided for generating and using a genetically engineered bacterium comprising a modification in a gene that regulates nitrogen fixation or assimilation, wherein the modification in the gene that regulates nitrogen fixation or assimilation results in one or more of the following: constitutive expression of a nifA gene under nitrogen-limiting and non-nitrogen-limiting conditions, nifA activity under non-nitrogen-limiting conditions, decreased GlnD uridylyltransferase activity, decreased GlnE adenylyl scavenging activity, and increased ammonium excretion.

UY0001039196A 2020-05-01 2021-05-03 MODIFIED BACTERIAL STRAINS TO IMPROVE NITROGEN FIXATION UY39196A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063019247P 2020-05-01 2020-05-01
PCT/US2020/031201 WO2021221690A1 (en) 2020-05-01 2020-05-01 Modified bacterial strains for improved fixation of nitrogen
PCT/US2021/013120 WO2021146209A1 (en) 2020-01-13 2021-01-12 Consortia of microorganisms for spatial and temporal delivery of nitrogen

Publications (1)

Publication Number Publication Date
UY39196A true UY39196A (en) 2021-11-30

Family

ID=78332201

Family Applications (1)

Application Number Title Priority Date Filing Date
UY0001039196A UY39196A (en) 2020-05-01 2021-05-03 MODIFIED BACTERIAL STRAINS TO IMPROVE NITROGEN FIXATION

Country Status (4)

Country Link
EP (1) EP4143211A2 (en)
CA (1) CA3172322A1 (en)
UY (1) UY39196A (en)
WO (1) WO2021222567A2 (en)

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CN112739668A (en) 2018-06-27 2021-04-30 皮沃特生物股份有限公司 Agricultural compositions comprising reconstituted nitrogen-fixing microorganisms
CN112322589A (en) * 2020-11-24 2021-02-05 吉林省农业科学院 Penicillium chrysogenum double-stranded RNA fungal virus for improving growth speed of beauveria bassiana hyphae
AR124494A1 (en) 2020-12-23 2023-04-05 Pivot Bio Inc AGRICULTURAL SYSTEMS, METHODS AND COMPOSITIONS FOR SEED TREATMENT
WO2023147050A1 (en) 2022-01-27 2023-08-03 Pivot Bio, Inc. Assessing relative plant nitrogen in a field environment
CN116083264A (en) * 2022-07-12 2023-05-09 华南农业大学 Kazakhstania strain H41 and application thereof
WO2024015230A1 (en) 2022-07-13 2024-01-18 Pivot Bio, Inc. Seed treatment systems, methods, and agricultural compositions
CN116790412B (en) * 2023-03-31 2024-03-29 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) Pseudomonas stutzeri XN05-1, application thereof and obtained plant salt-resistant microbial agent

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Also Published As

Publication number Publication date
WO2021222567A2 (en) 2021-11-04
WO2021222567A3 (en) 2021-12-09
CA3172322A1 (en) 2021-11-04
EP4143211A2 (en) 2023-03-08

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