UY32558A - Uso para genes de fimbrias polares largas de cepas patógenas de escherichia coli - Google Patents

Uso para genes de fimbrias polares largas de cepas patógenas de escherichia coli

Info

Publication number
UY32558A
UY32558A UY0001032558A UY32558A UY32558A UY 32558 A UY32558 A UY 32558A UY 0001032558 A UY0001032558 A UY 0001032558A UY 32558 A UY32558 A UY 32558A UY 32558 A UY32558 A UY 32558A
Authority
UY
Uruguay
Prior art keywords
genes
coli
escherichia coli
pathographic
fimbries
Prior art date
Application number
UY0001032558A
Other languages
English (en)
Inventor
Alfredo G Torres
Original Assignee
Univ Texas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Texas filed Critical Univ Texas
Publication of UY32558A publication Critical patent/UY32558A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/113—Cycle sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se proporcionan métodos para identificar genes Ipf en serotipos patogénicos de la familia de las Enterobacteriaceae y para diferenciar cepas de Escherichia coli (E. coli) 0157:H7 en un aislado, utilizando pares de iniciadores específicos para las variantes del gen Ipf, particularmente de los genes IpfA1 y/o IpfA2 y el tamaño de amplicón del producto de la PCR para identificar serotipos prototípicos de las Enterobacteriaceae o, más específicamente, para diferenciar cepas del serotipo de E. coli 0157:H7. La diferenciación requiere además la identificación del tipo de variante del gen eae del aislado de E. coli, el cual en combinación con la identificación de la variante lpfA1 y/o lpfA2 proporciona señaladores originales. También se proporcionan los pares de indicadores y un kit que los comprende.
UY0001032558A 2009-04-10 2010-04-12 Uso para genes de fimbrias polares largas de cepas patógenas de escherichia coli UY32558A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US21238509P 2009-04-10 2009-04-10

Publications (1)

Publication Number Publication Date
UY32558A true UY32558A (es) 2010-09-30

Family

ID=42936769

Family Applications (1)

Application Number Title Priority Date Filing Date
UY0001032558A UY32558A (es) 2009-04-10 2010-04-12 Uso para genes de fimbrias polares largas de cepas patógenas de escherichia coli

Country Status (4)

Country Link
US (1) US20120082989A1 (es)
AR (1) AR076356A1 (es)
UY (1) UY32558A (es)
WO (1) WO2010117465A2 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109988852A (zh) * 2019-04-17 2019-07-09 江苏省农业科学院 一种肠出血性大肠埃希菌o157:h7检测试剂盒及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6365723B1 (en) * 1998-12-04 2002-04-02 Wisconsin Alumni Research Foundation Sequences of E. coli O157
JP2002531130A (ja) * 1998-12-08 2002-09-24 チルドレンズ ホスピタル アンド レジオナル メディカル センター ＥｓｃｈｅｒｉｃｈｉａｃｏｌｉＯ１５７：Ｈ７と他の株とを識別する多形遺伝子座

Also Published As

Publication number Publication date
AR076356A1 (es) 2011-06-08
WO2010117465A2 (en) 2010-10-14
US20120082989A1 (en) 2012-04-05
WO2010117465A3 (en) 2011-06-16

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Legal Events

Date Code Title Description
109 Application deemed to be withdrawn

Effective date: 20200616