UY25687A1 - Metodo para identificar sustancias para el tratamiento de trastornos mediados por c-jun - Google Patents
Metodo para identificar sustancias para el tratamiento de trastornos mediados por c-junInfo
- Publication number
- UY25687A1 UY25687A1 UY25687A UY25687A UY25687A1 UY 25687 A1 UY25687 A1 UY 25687A1 UY 25687 A UY25687 A UY 25687A UY 25687 A UY25687 A UY 25687A UY 25687 A1 UY25687 A1 UY 25687A1
- Authority
- UY
- Uruguay
- Prior art keywords
- jun
- treatment
- jnk
- mutated
- substance
- Prior art date
Links
- 239000000126 substance Substances 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 4
- 230000001404 mediated effect Effects 0.000 title abstract 2
- 101001050288 Homo sapiens Transcription factor Jun Proteins 0.000 abstract 8
- 102100023132 Transcription factor Jun Human genes 0.000 abstract 8
- 108010055717 JNK Mitogen-Activated Protein Kinases Proteins 0.000 abstract 3
- 102000019145 JUN kinase activity proteins Human genes 0.000 abstract 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract 3
- 208000035475 disorder Diseases 0.000 abstract 3
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 230000010399 physical interaction Effects 0.000 abstract 2
- 235000018102 proteins Nutrition 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108700028369 Alleles Proteins 0.000 abstract 1
- 101150089023 FASLG gene Proteins 0.000 abstract 1
- 108010043121 Green Fluorescent Proteins Proteins 0.000 abstract 1
- 102000004144 Green Fluorescent Proteins Human genes 0.000 abstract 1
- 101000628949 Homo sapiens Mitogen-activated protein kinase 10 Proteins 0.000 abstract 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 abstract 1
- 102100026931 Mitogen-activated protein kinase 10 Human genes 0.000 abstract 1
- 230000005725 N terminal phosphorylation Effects 0.000 abstract 1
- 206010028980 Neoplasm Diseases 0.000 abstract 1
- 108091000080 Phosphotransferase Proteins 0.000 abstract 1
- 108700008625 Reporter Genes Proteins 0.000 abstract 1
- 235000004279 alanine Nutrition 0.000 abstract 1
- 201000011510 cancer Diseases 0.000 abstract 1
- 239000005090 green fluorescent protein Substances 0.000 abstract 1
- 230000001537 neural effect Effects 0.000 abstract 1
- 230000000626 neurodegenerative effect Effects 0.000 abstract 1
- 230000009223 neuronal apoptosis Effects 0.000 abstract 1
- 230000006548 oncogenic transformation Effects 0.000 abstract 1
- 201000008968 osteosarcoma Diseases 0.000 abstract 1
- 102000020233 phosphotransferase Human genes 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5058—Neurological cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/82—Translation products from oncogenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5041—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving analysis of members of signalling pathways
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2510/00—Detection of programmed cell death, i.e. apoptosis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Biophysics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Neurosurgery (AREA)
- Oncology (AREA)
- Gastroenterology & Hepatology (AREA)
- Neurology (AREA)
- General Engineering & Computer Science (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Método para identificar sustancias para el tratamiento de trastornos asociados con c-Jun, caracterizado porque se ensayan las sustancias en cuanto a su capacidad para inhibir la fosforilación N-terminal de c-Jun; en especial este método ensaya la capacidad de una sustancia para inhibir la interacción física de una quinasa N-terminal de c-Jun con un sustrato c-Jun; y más en particular se incuban con la sustancia de prueba el dominio de fijación para JNK (particularmente JNK3) en c-Jun (como fasL) y el dominio de fijación para c-Jun en una JNK, o proteínas que contienen dichos dominios, estando uno de ellos fusionado a un producto de gen informador (como una proteína con fluorescencia verde), y estando el otro inmovilizado sobre un soporte, y se mide el efecto de la sustancia sobre la interacción física de las dos proteínas midiendo la actividad del producto informador. Ejemplos de trastornos mediados por c-Jun y donde el método es de utilidad para el tratamiento de trastornos neurodegenerativos o neuronales como la apoptosis neuronal, de apoplejía, de una trasformación oncogénica como cáncer, o para el tratamiento de osteosarcoma. También se incluye un animal transgénico, caracterizado porque es homozigótico para un alelo mutado de c-Jun que tiene mutados los sitios de fosfoaceptor de JNK, en particular si tiene mutados ala serina 63 como la serina 73 a alanina.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP98116415A EP0984070A1 (en) | 1998-08-31 | 1998-08-31 | Method for identifying substances for the treatment of disorders associated with c-Jun-mediated apoptosis |
Publications (1)
Publication Number | Publication Date |
---|---|
UY25687A1 true UY25687A1 (es) | 2000-03-31 |
Family
ID=8232547
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
UY25687A UY25687A1 (es) | 1998-08-31 | 1999-08-31 | Metodo para identificar sustancias para el tratamiento de trastornos mediados por c-jun |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0984070A1 (es) |
AU (1) | AU5855899A (es) |
CO (1) | CO5090889A1 (es) |
UY (1) | UY25687A1 (es) |
WO (1) | WO2000012754A1 (es) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10036501B4 (de) * | 2000-07-25 | 2004-08-19 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | Verfahren zur Messung der JNK/SAPKs-Aktivität mittels der Bestimmung der Phosphorylierung von Transkriptionsfaktoren |
US7247767B2 (en) | 2004-01-21 | 2007-07-24 | Boehringer Ingelheim International Gmbh | Mouse model for psoriasis and psoriatic arthritis |
US20060148996A1 (en) | 2004-12-30 | 2006-07-06 | Coggio William D | Low refractive index fluoropolymer compositions having improved coating and durability properties |
US9028123B2 (en) | 2010-04-16 | 2015-05-12 | Flex Lighting Ii, Llc | Display illumination device with a film-based lightguide having stacked incident surfaces |
JP2013525955A (ja) | 2010-04-16 | 2013-06-20 | フレックス ライティング 2,エルエルシー | フィルムベースのライトガイドを備える照明デバイス |
WO2011130720A2 (en) | 2010-04-16 | 2011-10-20 | Flex Lighting Ii, Llc | Sign comprising a film-based lightguide |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB8916806D0 (en) * | 1989-07-22 | 1989-09-06 | Univ Wales Medicine | Modified proteins |
US6514745B1 (en) * | 1993-07-19 | 2003-02-04 | The Regents Of The University Of California | Oncoprotein protein kinase |
-
1998
- 1998-08-31 EP EP98116415A patent/EP0984070A1/en not_active Withdrawn
-
1999
- 1999-08-27 WO PCT/EP1999/006304 patent/WO2000012754A1/en active Application Filing
- 1999-08-27 CO CO99054518A patent/CO5090889A1/es unknown
- 1999-08-27 AU AU58558/99A patent/AU5855899A/en not_active Abandoned
- 1999-08-31 UY UY25687A patent/UY25687A1/es not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
CO5090889A1 (es) | 2001-10-30 |
WO2000012754A8 (en) | 2000-08-10 |
AU5855899A (en) | 2000-03-21 |
WO2000012754A1 (en) | 2000-03-09 |
EP0984070A1 (en) | 2000-03-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
109 | Application deemed to be withdrawn |
Effective date: 20110726 |