USRE48137E1 - Multivalent vaccine protection from Staphylococcus aureus infection - Google Patents

Multivalent vaccine protection from Staphylococcus aureus infection Download PDF

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USRE48137E1
USRE48137E1 US15/903,831 US201315903831A USRE48137E US RE48137 E1 USRE48137 E1 US RE48137E1 US 201315903831 A US201315903831 A US 201315903831A US RE48137 E USRE48137 E US RE48137E
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aureus
seq
set forth
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biofilm
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Mark Shirtliff
Janette Harro
Jeffrey Leid
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University of Maryland Baltimore
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/09—Lactobacillales, e.g. aerococcus, enterococcus, lactobacillus, lactococcus, streptococcus
    • A61K39/092—Streptococcus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/085—Staphylococcus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • A61K38/14—Peptides containing saccharide radicals; Derivatives thereof, e.g. bleomycin, phleomycin, muramylpeptides or vancomycin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/70—Multivalent vaccine

Definitions

  • the present invention relates to multivalent vaccine formulations effective against Staphylococcus aureus, including both biofilm and planktonic types of bacterial infections, and to methods of using the formulations in the treatment and prevention of S. aureus infections in subjects.
  • MRSA Methicillin-resistant S. aureus
  • CA-MRSA methicillin-resistance
  • S. aureus is also the major mediator of prosthetic implant infection (1, 54).
  • Treating prosthetic implant infections is a complicated process, and a number of staphylococcal defense mechanisms may be responsible for this difficulty as well as the capacity of S. aureus to evade clearance by the host immune response.
  • One of the most important mechanisms utilized by S. aureus to thwart the host immune response and develop into a persistent infection is through the formation of a highly-developed biofilm.
  • a biofilm is defined as a microbe-derived community in which bacterial cells are attached to a hydrated surface and embedded in a polysaccharide matrix (13).
  • Bacteria in a biofilm exhibit an altered phenotype in their growth, gene expression, and protein production (17), and prosthetic medical devices are often a site of chronic infection, because they present a suitable substrate for bacterial adherence, colonization, and biofilm formation.
  • Biofilm formation by S. aureus during prosthetic implant infection makes eradication of this bacteria extremely difficult, due in part to the dramatically increased resistance of bacteria in a biofilm to host defenses (21) and to antibiotics (46, 51), compared to their planktonic counterparts.
  • aureus virulence factors differential protein expression during different modes of growth (exponential growth versus stationary) or type of infection (planktonic versus biofilm), and the lack of antigen conservation amongst relevant clinical isolates.
  • StaphVAX polysaccharide vaccine developed using the S. aureus capsular polysaccharide 5 (CP5) and capsular polysaccharide 8 (CP8) conjugated to the Pseudomonas aeruginosa exotoxoid A failed to provide protection in phase III clinical trials against S. aureus-mediated bacteremia in two different cohorts of 1804 and 3600 hemodialysis patients (59).
  • subunit vaccines developed against the clumping factor A (C1fA) (2, 27), clumping factor B (C1fB) (57), fibronectin binding protein (FnBP) (65), ⁇ -Hemolysin (9, 29), Panton-Valentine leukocidin (PVL) (8), and the iron-regulated surface determinant B (IsdB) (30, 33) mediate partial protection in experimental animal models.
  • These subunit vaccines did not provide complete protection, despite the candidate proteins being highly immunogenic in vivo (25, 33, 57) and the resultant antibodies promoting op sonic killing of S. aureus (65).
  • S. aureus has nearly 70 virulence factors and functional redundancy amongst these factors may abrogate the effect of neutralizing one factor.
  • S. aureus has nearly 70 virulence factors and functional redundancy amongst these factors may abrogate the effect of neutralizing one factor.
  • aureus expresses multiple iron acquisition systems: siderophores staphyloferrin A and B transport transferrin to receptors HtsA and SirA (14, 43), an ABC transporter Fhu imports Fe 3+ hydroxamates (58), and iron-regulated surface determinant (Isd) B and IsdH receptors that bind hemoglobin/haptoglobin complexes (18, 62), therefore the overall effectiveness of anti-IsdB antibodies that block IsdB-mediated hemoglobin binding may be only a modest effect on iron uptake and the organism's pathogenicity (30).
  • Efficacy of a monovalent vaccine can also be compromised by differential expression of the targeted protein during the course of infection. While S. aureus initiates colonization by binding host extracellular ligands using its adhesin proteins called microbial surface components recognizing adhesive matrix molecules (MSCRAMMs), including the fibronectin-binding protein (FnBP), these factors are mostly down-regulated as the sessile bacteria encapsulate themselves in an extracellular polysaccharide matrix, or biofilm (44, 55). Hence, vaccines designed to target a MSCRAMM will be ineffective at clearance after the bacteria transition into the biofilm phenotype. Evaluation of the MSCRAMM FnBP vaccine demonstrated it provided partial protection against S.
  • MSCRAMMs microbial surface components recognizing adhesive matrix molecules
  • FnBP fibronectin-binding protein
  • S. aureus in a murine model of sepsis, but the study failed to enumerate bacteria in the blood and/or kidneys to verify bacterial clearance. It is feasible that S. aureus can subvert the humoral response to FnBP, form a sessile biofilm and down-regulate FnBP, and become completely recalcitrant the host response.
  • a vaccine strategy that circumvents the incomplete protection of monovalent vaccines caused by protein redundancy, differential protein expression, or isolate-specific genetic divergence is the generation of a multifactorial assault using a multivalent subunit vaccine.
  • Stranger-Jones et al. demonstrated a quadrivalent vaccine comprised of surface-exposed proteins: iron-regulated surface determinant A (IsdA), IsdB, and serine aspartate repeat protein D (SdrD), and SdrE increased survival rates against S. aureus-mediated lethal challenge compared to protection afforded by each monovalent variant (61). Although the authors stressed the survival rates after lethal challenge, they omitted enumeration of S.
  • the multivalent vaccine had limited efficacy, providing complete protection against only two of five clinical S. aureus isolates tested (61).
  • comparative analysis of multiple S. aureus genomes found a lack of conservation amongst some surface proteins, including SdrD and SdrE (39), which indicates the limited efficacy of the IsdA/IsdB/SdrD/SdrE vaccine formulation may extend beyond the clinical isolates tested by Stranger-Jones.
  • Vaccine studies have predominately focused on protection against planktonic-mediated infection by examining sepsis (20, 27, 33, 38, 41, 61, 65) or pneumonia (9), while few studies have incidentally evaluated protection, mediated by popular vaccine candidates, against biofilm infection with experimental endocarditis (2), skin (8, 22, 29), or abscess models (20, 61).
  • Brady et al. focused on identifying biofilm upregulated proteins that are immunogenic (4) and established that a multivalent biofilm-based vaccine when coupled with vancomycin treatment could eradicate a biofilm infection, which is traditionally recalcitrant to clearance by either antibiotic treatment or immune response (5).
  • PNAG poly-N-acetyl- ⁇ -1,6-glucosamine
  • dPNAG deacetylated form of PNAG
  • dPNAG deacetylated form of PNAG
  • GlcNH 2 a synthetic 9-mer of ⁇ -(1 ⁇ 6)-D-glucosamine conjugated to tetanus toxoid
  • PIA is generated by enzymes encoded on the icaABDC locus (28), but the presence of the icaABDC locus does not directly correlate to biofilm formation in vitro (32) and the icaABDC locus in S.
  • aureus was dispensable in a subset of in vivo orthopedic prosthesis-associated and catheter-associated infections, which are identified as biofilm-mediated infections (53). While the efficacy of the PNAG vaccine against S. aureus biofilms requires further evaluation, the dispensability of the icaABDC locus in some S. aureus strains isolated from clinical infections suggests that the PNAG vaccine would provide limited protection against S. aureus biofilm infections.
  • Another consideration for vaccine development is the type of response elicited by the host immune system and the ability of the pathogen to subvert immune mediators using immunoavoidance factors, which may have varied outcomes depending on the host environment.
  • the immune response elicited in vitro against S. aureus or its virulence factors, specifically staphylococcal enterotoxin A or B and the alpha toxin, is a pro-inflammatory Th1-response (3, 7, 15, 42).
  • Th1-biased C57BL/6J mice were resistant and Th2-biased BALB/c mice were susceptible to this acute form of S. aureus infection (63).
  • Th-1 Th-1 response was elicited against a S. aureus implant infection in C57BL/6J mice, but the mice were susceptible and developed a chronic infection with 10 7 CFU/tibia at 49 days post-infection (45).
  • the S. aureus biofilm appears to be recalcitrant to the pro-inflammatory response, which damages host tissue at the infection site generating devitalized sites for S. aureus to colonize.
  • Th-2 biased BALB/c mice were resistant to the S. aureus implant infection, and ablation of interleukin-4 or the depletion of Treg cells abrogated the protection against S. aureus in BALB/c mice (46).
  • Th2-mediated resistance to bacterial infection was also revealed for subcutaneous infections with S. aureus, where higher bacterial loads were observed in C57BL/6J mice versus BALB/c mice (45).
  • Increased CXCL-2 expression in the C57BL/6J mice correlated with the susceptibility to subcutaneous infection (45), and may halt the killing activity of polymorphonuclear neutrophils (PMNs) after influx and internalization of S. aureus (23).
  • PMNs polymorphonuclear neutrophils
  • Additional vaccine formulations would add to the arsenal of means used to treat and/or prevent S. aureus infections.
  • Staphylococcus aureus has re-emerged as a major human pathogen and there are presently no vaccines that afford consistent, long-term protection against S. aureus infections. While infections, particularly those with MRSA, are often nosocomial in origin, community acquired infections associated with this microbial species have reached epidemic levels.
  • infections particularly those with MRSA
  • community acquired infections associated with this microbial species have reached epidemic levels.
  • One of the ways in which S. aureus is able to persist in the host and remain recalcitrant to clearance by the immune system or antimicrobial agents is through a biofilm mode of growth. Therefore, an effective vaccine and/or treatment modality that could prevent the establishment of biofilm-mediated chronic infections by S. aureus is needed.
  • the present invention demonstrates protection against biofilm-associated S. aureus infection through the use of a multi-component vaccine, alone or in combination with subsequent antimicrobial agent therapy. Complete protection was demonstrated in a murine tibial implant model using a biofilm- and planktonic-specific pentavalent vaccine, with 100% clearance of S. aureus.
  • the vaccine formulations of the present invention hold significant promise for those with identified risk factors for S. aureus biofilm infection. Even in patients that acquire a S. aureus infection, an anti-biofilm vaccine could allow these previously untreatable infections to be halted or cured without the need for surgical intervention.
  • the present invention thus provides new means to limit and eradiate S. aureus biofilm infections that could help to prevent the onset of chronic disease, saving patients from significant morbidity and mortality.
  • the present invention is directed to the following embodiments of vaccine formulations.
  • the present invention is directed to a vaccine formulation comprising five different polypeptides of a strain of S. aureus (a first, second, third, fourth and fifth polypeptide of a strain of S. aureus), or portions thereof, or variants thereof, or combinations thereof, and a pharmaceutically acceptable carrier or diluent.
  • the strain of S. aureus may be a methicillin-resistant or a methicillin-sensitive strain of S. aureus.
  • At least one of the S. aureus polypeptides is a polypeptide expressed by a planktonic form of the bacteria and at least one of the S. aureus polypeptides is a polypeptide expressed by a biofilm form of the bacteria.
  • one of the S. aureus polypeptides is a polypeptide expressed by a planktonic form of the bacteria and four of the S. aureus polypeptides are polypeptides expressed by a biofilm form of the bacteria.
  • first, second, third, fourth and fifth polypeptides are S. aureus polypeptide SA0037 set forth in SEQ ID NO:13, S. aureus polypeptide SA0119 set forth in SEQ ID NO:14, S. aureus polypeptide SA0486 set forth in SEQ ID NO:15, S. aureus polypeptide SA0688 set forth in SEQ ID NO:16, and S. aureus glucosaminidase set forth in SEQ ID NO:17.
  • the vaccine formulations comprise one or more portions of one or more of the S. aureus polypeptides, wherein the portions individually encompass at least about 20 contiguous amino acids of the full length polypeptide.
  • the vaccine formulations comprise one or more variants of one or more of the S. aureus polypeptides or portions thereof, wherein the variants individually have at least about 95% identity to a S. aureus polypeptide or portion thereof.
  • the present invention is directed to a vaccine formulation comprising five different, full-length polypeptides of a strain of S. aureus.
  • the five polypeptides are S. aureus polypeptide SA0037 set forth in SEQ ID NO:13, S. aureus polypeptide SA0119 set forth in SEQ ID NO:14, S. aureus polypeptide SA0486 set forth in SEQ ID NO:15, S. aureus polypeptide SA0688 set forth in SEQ ID NO:16, and S. aureus glucosaminidase set forth in SEQ ID NO:17.
  • the present invention is also directed to the following embodiments of methods of using the vaccine formulations of the invention.
  • the present invention is directed to methods of generating an immune response in a subject comprising administering an immunologically effective amount of a vaccine formulation of the present invention to a subject, thereby generating an immune response in a subject.
  • the immune response is a protective immune response.
  • the present invention is directed to methods for treating a S. aureus infection in a subject, comprising administering a therapeutically effective amount of a vaccine formulation of the present invention to a subject having a S. aureus infection, thereby treating a S. aureus infection in a subject.
  • the present invention is directed to methods of inhibiting a S. aureus infection in a subject, comprising administering a therapeutically effective amount of a vaccine formulation of the present invention to a subject at risk of developing a S. aureus infection, thereby inhibiting a S. aureus infection in a subject.
  • the methods for treating or inhibiting a S. aureus infection may further comprise administering one or more antimicrobial agents to a subject having a S. aureus infection or at risk of developing a S. aureus infection, wherein the antimicrobial agent is administered prior to, concurrent with or after the vaccine formulation.
  • the antimicrobial agent(s) may be selected from the group that includes, but is not limited to, an Aminoglycoside, such as Amikacin, Gentamicin, Kanamycin, Neomycin, Netilmicin, Streptomycin, Tobramycin or Paromomycin; a Carbacephem, such as Loracarbef; a Carbapenem, such as Ertapenem, Doripenem, Imipenem/Cilastatin or Meropenem; a Cephalosporin, such as Cefadroxil, Cefazolin, Cefalotin, Cefalexin, Cefaclor, Cefamandole, Cefoxitin, Cefprozil, Cefuroxime, Cefixime, Cefdinir, Cefditoren, Cefoperazone, Cefotaxime, Cefpodoxime, Ceftazidime, Ceftibuten, Ceftizoxime, Ceftriaxone, Cefe
  • the S. aureus infection may be any S. aureus infection of a subject, including, for example, one or more of a S. aureus biofilm infection, a planktonic S. aureus infection, a S. aureus osteomyelitis infection, a biofilm-associated S. aureus osteomyelitis infection, a S. aureus indwelling medical device infection, a S. aureus endocarditis infection, a S. aureus diabetic wound or ulcer infection, a S. aureus chronic rhinosinusitis infection, a S. aureus ventilator associated pneumonia infection, a S. aureus intravenous catheter associated infection, a S.
  • aureus skin infection a S. aureus nectrotizing fasciitis, a S. aureus keratitis, a S. aureus endophthlamitis, a S. aureus pyopneumothorax, a S. aureus empyema, and a S. aureus septicemia.
  • FIG. 2 Vaccination with quadrivalent vaccine and adjunctive vancomycin treatment in a rabbit model of an S. aureus osteomyelitis biofilm infection.
  • FIG. 3 Vaccination with quadrivalent biofilm vaccine, planktonic vaccine, or pentavalent dual phenotype vaccine in a murine model of a S. aureus implant infection.
  • Control mice received no treatment (column 1) or unvaccinated with Alum alone (column 2).
  • Experimental mice received a biofilm-directed quadrivalent vaccine (column 3), a planktonic-specific monovalent vaccine (SA0119; column 4), or a combination of the antigens in a pentavalent vaccine (column 5).
  • Biofilm-embedded bacteria have remarkably different phenotypic and antigenic properties compared to their free-floating, planktonic counterparts. These differences have presented a struggle when designing vaccine formulations for use in treating and preventing both types of bacterial infections. Even individual stages of biofilm growth (from early attached to maturing and fully mature stages) have been shown to be more antigenically distinct from one another than even biofilm versus planktonic bacteria (66).
  • biofilm and planktonic antigens that are expressed on the membrane or cell wall into a multivalent vaccine, protection of the host against microbial challenge by the specific microbial species can be elicited. This protection can be promoted since bacteria in the host exist in antigenically distinct forms of the planktonic and biofilm modes of growth during an infection and, as a result, a dual immune response against both phenotypes must be produced in the host.
  • the vaccine formulations of the present invention include antigens effective at priming the host immune response to clear both detached, free-floating populations of bacteria as well as bacteria forming a biofilm type of infection. This work is the first to acknowledge, and overcome, the differences of protein expression within different types of infection caused by the same microorganism, and demonstrate (as shown in the Examples) complete clearance in an S. aureus animal model of infection instead of only a significant reduction in bacterial populations.
  • the present invention relates to vaccine formulations effective against S. aureus, including methicillin-resistant S. aureus (MRSA) and methicillin-sensitive S. aureus (MSSA), and to methods of using the vaccines in the treatment and prevention of S. aureus infections in a subject.
  • MRSA methicillin-resistant S. aureus
  • MSSA methicillin-sensitive S. aureus
  • the vaccine formulations of the present invention comprise at least a portion of each of five different polypeptides of a strain of S. aureus and a pharmaceutically acceptable carrier or diluent.
  • the vaccine formulations are characterized in that they comprise at least one S. aureus polypeptide expressed by a planktonic form of the bacteria and at least one S. aureus polypeptide expressed by a biofilm form of the bacteria.
  • the vaccine formulations of the present invention may thus comprise one, two, three, or four S. aureus polypeptides expressed by a planktonic form of the bacteria, and one, two, three, or four S. aureus polypeptides expressed by a biofilm form of the bacteria.
  • the vaccine formulations comprise one S. aureus polypeptide expressed by a planktonic form of the bacteria and four S. aureus polypeptides expressed by a biofilm form of the bacteria.
  • the formulations may comprise only full-length versions of the polypeptides.
  • the formulations may comprise only portions of the full-length polypeptides.
  • the formulations may comprise a combination of portions and full-length polypeptides.
  • combinations include formulations having one, two, three, four, five, six or more different portions of the same S. aureus polypeptide in combination with one or more portions of other polypeptides and/or full-length polypeptides and/or both portions and full-length versions of the same polypeptide.
  • each of the formulations comprises at least one portion of each of five different polypeptides of a strain of S. aureus.
  • planktonic- and biofilm-expressed polypeptides included in the vaccine formulations of the present invention is not particularly limited but each is a polypeptide from a strain of S. aureus.
  • the primary purpose of the vaccine formulations is to prime and activate the immune system of the subject receiving the vaccine formulation, the use of polypeptides exposed on the surface of the bacteria is particularly preferred.
  • the polypeptides may be cell wall and cell wall-associated polypeptides of S. aureus. Examples of such polypeptides include the S.
  • aureus polypeptides SA0037 SEQ ID NO:13
  • SA0119 SEQ ID NO:14
  • SA0486 SEQ ID NO:15
  • SA0688 SEQ ID NO:16
  • glucosaminidase SEQ ID NO:17
  • S. aureus polypeptides that may be used in the vaccine formulations of the present invention include the polypeptides of Table 1.
  • the size of the peptide is only limited by its ability to be recognized by the immune system of the subject to which the vaccine is administered.
  • the peptides included in the formulations should be about 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or more contiguous amino acids of the full-length protein.
  • the preferred size of the peptides is between about 20 amino acids and 3000 amino acids in length, more preferably between about 40 amino acids and 1500 amino acids in length, even more preferably between about 150 amino acids and 1300 amino acids in length.
  • the peptides may 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% of the size of the full-length protein.
  • the polypeptides and portions thereof used in the formulations of the present invention are from strains of S. aureus.
  • strains of S. aureus There is no limitation on the different strains of S. aureus that might be used.
  • polypeptides from medically important strains of S. aureus such methicillin-resistant S. aureus (either community-associated or hospital-acquired strains) and methicillin-sensitive S. aureus, may be used to constitute the vaccine formulations of the present invention. Therefore, the vaccine formulations of the present invention include the use of variants of the S.
  • Sequence identity is determined by aligning the amino acid sequence of two peptides or proteins and calculating the number of amino acid differences over the entire length of the alignment. The skilled artisan will understand that there are a number of commercially available sequence manipulation programs for use in making such calculations, including the website of the National Center for Biotechnology Information.
  • polypeptides, portions, and variants thereof used in the vaccine formulations may be obtained through any of the many well-established means known in the art.
  • proteins can possess the native glycosylation of polypeptide as it is produced by the corresponding strain of S. aureus, or they can lack such glycosylation, or they can have altered glycosylation.
  • Vaccine Components Carriers and Excipients
  • the pharmaceutically acceptable carrier, diluent or excipient included in the vaccine formulations will vary based on the identity of the proteins in the formulation, the means used to administer the formulation, the site of administration and the dosing schedule used.
  • Suitable examples of carriers and diluents are well known to those skilled in the art and include water-for-injection, saline, buffered saline, dextrose, water, glycerol, ethanol, propylene glycol, polysorbate 80 (Tween-80TM), poly(ethylene)glycol 300 and 400 (PEG 300 and 400), PEGylated castor oil (e.g. Cremophor EL), poloxamer 407 and 188, hydrophilic and hydrophobic carriers, and combinations thereof.
  • Hydrophobic carriers include, for example, fat emulsions, lipids, PEGylated phospholipids, polymer matrices, biocompatible polymers, lipospheres, vesicles, particles, and liposomes. The terms specifically exclude cell culture medium. Additional carriers include cornstarch, gelatin, lactose, sucrose, microcrystalline cellulose, kaolin, mannitol, dicalcium phosphate, sodium chloride, alginic acid, croscarmellose sodium, and sodium starch glycolate.
  • Excipients included in a formulation have different purposes depending, for example on the nature of the vaccine formulation and the mode of administration.
  • examples of generally used excipients include, without limitation: stabilizing agents, solubilizing agents and surfactants, buffers, antioxidants and preservatives, tonicity agents, bulking agents, lubricating agents, emulsifiers, suspending or viscosity agents, inert diluents, fillers, disintegrating agents, binding agents, wetting agents, lubricating agents, antibacterials, chelating agents, sweetners, perfuming agents, flavouring agents, coloring agents, administration aids, and combinations thereof.
  • intramuscular preparations can be prepared and administered in a pharmaceutically acceptable diluent such as Water-for-Injection, 0.9% saline, or 5% glucose solution.
  • a pharmaceutically acceptable diluent such as Water-for-Injection, 0.9% saline, or 5% glucose solution.
  • the vaccine formulations exist as atomized dispersions for delivery by inhalation.
  • the atomized dispersion of the vaccine formulation typically contains carriers common for atomized or aerosolized dispersions, such as buffered saline and/or other compounds well known to those of skill in the art.
  • the delivery of the vaccine formulations via inhalation has the effect of rapidly dispersing the vaccine formulation to a large area of mucosal tissues as well as quick absorption by the blood for circulation.
  • a method of preparing an atomized dispersion is described in U.S. Pat. No. 6,187,344, entitled, “Powdered Pharmaceutical Formulations Having Improved Dispersibility,” which is hereby incorporated by reference in its entirety.
  • the vaccines and vaccine formulations may also be administered in a liquid form.
  • the liquid can be for oral dosage, for ophthalmic or nasal dosage as drops, or for use as an enema or douche.
  • the vaccine formulation is formulated as a liquid, the liquid can be either a solution or a suspension of the vaccine formulation.
  • suitable formulations for the solution or suspension of the vaccine formulation are well know to those of skill in the art, depending on the intended use thereof.
  • Liquid formulations for oral administration prepared in water or other aqueous vehicles may contain various suspending agents such as methylcellulose, alginates, tragacanth, pectin, kelgin, carrageenan, acacia, polyvinylpyrrolidone, and polyvinyl alcohol.
  • the liquid formulations may also include solutions, emulsions, syrups and elixirs containing, together with the active compound(s), wetting agents, sweeteners, and coloring and flavoring agents.
  • Various liquid and powder formulations can be prepared by conventional methods for inhalation into the lungs of the mammal to be treated.
  • the vaccine formulations of the present invention may also include an adjuvant.
  • Suitable adjuvants include Freund's Complete and Incomplete Adjuvant, Titermax, Oil in Water Adjuvants, as well as Aluminum compounds where antigens, normally proteins, are physically precipitated with hydrated insoluble salts of aluminum hydroxide or aluminum phosphate.
  • Other adjuvants include liposome-type adjuvants comprising spheres having phospholipid bilayers that form an aqueous compartment containing the vaccine candidate and protecting it from rapid degradation, and that provide a depot effect for sustained release.
  • Surface active agents may also be used as adjuvants and include lipoteichoic acid of gram-positive organisms, lipid A, and TDM.
  • Quil A and QS-21 are suitable adjuvants that have hydrophilic and hydrophobic domains from which their surface-active properties arise.
  • Compounds normally found in the body such as vitamin A and E, and lysolecithin may also be used as surface-active agents.
  • Other classes of adjuvants include glycan analog, coenzyme Q, amphotericin B, dimethyldioctadecylammonium bromide (DDA), levamisole, and benzimidazole compounds.
  • the immunostimulation provided by a surface active agent may also be accomplished by either developing a fusion protein with non-active portions of the cholera toxin, exotoxin A, or the heat labile toxin from E. coli Immunomodulation through the use of anti-IL-17, anti IFN- ⁇ , anti-IL-12, IL-2, IL-10, or IL-4 may also be used to promote a strong Th2 or antibody mediated response to the vaccine formulation.
  • the present invention is also directed to methods of generating an immune response in a subject to a vaccine formulation of the present invention.
  • the present invention is directed to methods of generating an immune response in a subject, comprising administering an immunologically effective amount of a vaccine formulation of the present invention to a subject, thereby generating an immune response in a subject.
  • the immune response is preferably a protective immune response.
  • An “immunologically effective amount” of a vaccine formulation is one that is sufficient to induce an immune response to vaccine components in the subject to which the vaccine formulation is administered.
  • a “protective immune response” is one that confers on the subject to which the vaccine formulation is administered protective immunity against S. aureus.
  • the protective immunity may be partial or complete immunity.
  • the present invention is also directed to methods of treating a S. aureus infection in a subject using the vaccine formulations of the present invention.
  • the present invention is directed to methods of treating a S. aureus infection in a subject, comprising administering a therapeutically effective amount of a vaccine formulation of the present invention to a subject having a S. aureus infection, thereby treating a S. aureus infection in a subject.
  • the method further comprises administering an antimicrobial agent to the subject having a S. aureus infection in conjunction with the administration of the vaccine formulation.
  • the vaccine formulations of the present invention may also be used in methods of inhibiting a S. aureus infection in a subject. Such methods comprise administering a therapeutically effective amount of a vaccine formulation of the present invention to a subject at risk of developing a S. aureus infection, thereby inhibiting a S. aureus infection in a subject. In certain aspects, the method further comprises administering an antimicrobial agent to the subject at risk of developing a S. aureus infection in conjunction with the administration of the vaccine formulation.
  • a “therapeutically effective amount” of a vaccine formulation is one that is sufficient to provide at least some reduction in the symptoms of a S. aureus infection in a subject to which the vaccine formulation is administered, or one that is sufficient to achieve the goal of the method.
  • treating and “treatment” have their ordinary and customary meanings, and include one or more of, ameliorating a symptom of a S. aureus infection in a subject, blocking or ameliorating a recurrence of a symptom of a S. aureus infection in a subject, decreasing in severity and/or frequency a symptom of a S. aureus infection in a subject, as stasis, decreasing, or inhibiting growth of S. aureus in a subject.
  • Treatment means ameliorating, blocking, reducing, decreasing or inhibiting by about 1% to about 100% versus a subject to which the vaccine formulation of the present invention has not been administered (with or without the additional administration of the antimicrobial agent).
  • the ameliorating, blocking, reducing, decreasing or inhibiting is 100%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10%, 5% or 1%.
  • the treatment may begin prior to, concurrent with, or after the onset of clinical symptoms of the infection.
  • the results of the treatment may be permanent, such as where the S. aureus infection is completely cleared from the subject, or may be for a period of days (such as 1, 2, 3, 4, 5, 6 or 7 days), weeks (such as 1, 2, 3 or 4 weeks) or months (such as 1, 2, 3, 4, 5, 6 or more months).
  • the terms “inhibit”, “inhibiting” and “inhibition” have their ordinary and customary meanings, and include one or more of inhibiting colonization of S. aureus, inhibiting growth of S. aureus (all forms, including planktonic and biofilm) and inhibiting propagation of S. aureus.
  • Such inhibition is an inhibition of about 1% to about 100% versus a subject to which the vaccine formulation of the present invention has not been administered (with or without the additional administration of the antimicrobial agent).
  • the inhibition is an inhibition of 100%, 99%, 98%, 97%, 96%, 95%, 90%, 80%, 70%, 60%, 50%, 40%, 30%, 20%, 10%, 5% or 1%.
  • the inhibition lasts at least a period of days, weeks, months or years upon completing of the dosing schedule.
  • the inhibition is for the lifespan of the subject.
  • the methods for treating or inhibiting a S. aureus infection may further comprise administering one or more antimicrobial agents to a subject having a S. aureus infection or at risk of developing a S. aureus infection.
  • an antimicrobial agent may be included in the methods of the present invention, the antimicrobial agent may be administered prior to, concurrent with or after the vaccine formulation is administered to the subject.
  • the period of time between when the antimicrobial agent and the vaccine formulation are administered may be a period of hours (such as 6, 12, 18 or 24 hours), days (such as 1, 2, 3, 4, 5, 6 or 7 days), weeks (such as 1, 2, 3 or 4 weeks) or months (such as 1, 2, 3, 4, 5, 6 or more months).
  • the antimicrobial agent may be any that is effective in the treatment of a S. aureus infection and may include, but is not limited to, an Aminoglycoside, such as Amikacin, Gentamicin, Kanamycin, Neomycin, Netilmicin, Streptomycin, Tobramycin or Paromomycin; a Carbacephem, such as Loracarbef; a Carbapenem, such as Ertapenem, Doripenem, Imipenem/Cilastatin or Meropenem; a Cephalosporin, such as Cefadroxil, Cefazolin, Cefalotin, Cefalexin, Cefaclor, Cefamandole, Cefoxitin, Cefprozil, Cefuroxime, Cefixime, Cefdinir, Cefditoren, Cefoperazone, Cefotaxime, Cefpodoxime, Ceftazidime, Ceftibuten, Ceftizoxime, Ceftri
  • the vaccine formulations are administered in a pharmaceutically acceptable form and in substantially non-toxic quantities.
  • the vaccine formulations may be administered to a subject using different dosing schedules, depending on the particular use to which the formulations are put (e.g., administration to the subject pre- or post-exposure to S. aureus), the age and size of the subject, and the general health of the subject, to name only a few factors to be considered.
  • the vaccine formulations may be administered once, or twice, three times, four times, five times, six times or more, over a dosing schedule.
  • the timing between each dose in a dosing schedule may range between a few hours, six, 12, or 18 hours, or 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or more days.
  • the same quantity of protein in the formulation may be administered in each dose of the dosing schedule, or the amounts in each dose may vary.
  • the identity of the particular peptides and polypeptides in the formulation may also vary or remain the same in each dose in a dosing schedule.
  • the amount of protein administered to a subject in a dose when the methods of the present invention are practiced will vary based on the particular methods being practiced (e.g., prevention versus treatment of a S. aureus infection), the means and formulation of administration, the age and size of the subject, and the general health of the subject, to name only a few factors to be considered. In general, however, the amount of S. aureus protein administered to a subject in a dose will be sufficient to induce or boost an immune response in a subject to the components of the vaccine.
  • the vaccines formulations may contain between about 1 to about 1000 ug of total S. aureus protein per kg of body weight of the subject to which the dose of the vaccine formulation will be administered, more preferably between about 10 to about 200 ug, even more preferably between about 15 to about 100 ug.
  • Appropriate doses and dosing schedules can readily be determined by techniques well known to those of ordinary skill in the art without undue experimentation. Such a determination will be based, in part, on the tolerability and efficacy of a particular dose.
  • Administration of the vaccine formulations may be via any of the means commonly known in the art of vaccine delivery.
  • routes include intravenous, intraperitoneal, intramuscular, subcutaneous and intradermal routes of administration, as well as nasal application, by inhalation, ophthalmically, orally, rectally, vaginally, or by any other mode that results in the vaccine formulation contacting mucosal tissues.
  • the S. aureus infection may be any S. aureus infection of a subject, including, for example, one or more of a S. aureus biofilm infection, a planktonic S. aureus infection, a S. aureus osteomyelitis infection, a biofilm-associated S. aureus osteomyelitis infection, a S. aureus indwelling medical device infection, a S. aureus endocarditis infection, a S. aureus diabetic wound or ulcer infection, a S. aureus chronic rhinosinusitis infection, a S. aureus ventilator associated pneumonia infection, a S. aureus intravenous catheter associated infection, a S. aureus skin infection, a S.
  • aureus nectrotizing fasciitis a S. aureus keratitis, a S. aureus endophthlamitis, a S. aureus pyopneumothorax, a S. aureus empyema, and a S. aureus septicemia.
  • subject is intended to mean an animal, such birds or mammals, including humans and animals of veterinary or agricultural importance, such as dogs, cats, horses, sheep, goats, and cattle.
  • kits comprising the necessary components of a vaccine formulation that elicits an immune response to a strain of S. aureus and instructions for its use is also within the purview of the present invention.
  • mice Inbred C57BL/6 (6-8 weeks old) were purchased from Jackson Laboratories (Bar Harbor, Me.). Mice were maintained under micro-isolator conditions in the animal facility at the University of Maryland Dental School (Baltimore, Md.), in accordance with protocols reviewed and approved by the Institutional Animal Care and Use Committee (IACUC).
  • IACUC Institutional Animal Care and Use Committee
  • S. aureus used in these experiments, MRSA-M2
  • MRSA-M2 is a clinical isolate obtained from an osteomyelitis patient undergoing treatment at the University of Texas Medical Branch (Galveston, Tex.) and has been used in previous biofilm molecular analyses and animal infection models (5, 34, 37, 60) (6, 47, 49, 50).
  • An overnight S. aureus Tryptic Soy Broth (TSB) culture grown at 37° C. with 250 rpm shaking was diluted 1:100 in fresh, prewarmed TSB and incubated for 2 h at 37° C. with 250 rpm shaking. Cells were centrifuged, rinsed with PBS, counted via a Petroff Hausser counter, and diluted to 1 ⁇ 10 6 CFU/ml.
  • Candidate antigens selected from Brady et al. (5) were amplified using the primers listed in Table 2.
  • PCR products were cloned into pBAD-Thio/TOPO (SACOL0037 and SACOL0119) or pASK-IBA14 (SACOL0486, SACOL0688, and glucosaminidase), transformed into TOP10 E. coli, and sequenced. Details regarding the plasmids are provided in Table 3.
  • Plasmid Genotype or Characteristics Source pBAD-Thio/TOPO 4454 bp Invitrogen Life pUC ori, Amp R , pBAD Technologies promoter, for arabinose-inducible expression of PCR product pASK-IBA14 3001 bp IBA, pUC ori, Amp R , tetA promoter, Gottingen, for tetracycline-inducible Germany expression of PCR product
  • SACOL0037 and SACOL0119 were purified via ProBond cobalt affinity chromatography (Invitrogen, Life technologies, Carlsbad, Calif.), while all other antigens were purified using Strep-Tactin Superflow Columns (IBA, Gottingen, Germany). Purity was confirmed by resolving each protein on 10-20% SDS-PAGE and quantities were determined by BCA (Pierce, Rockford Ill.).
  • Desalting and buffer exchange to phosphate-buffered saline (PBS) was performed for SACOL0486, SACOL0688, and glucosaminidase using 30 kDa molecular weight cut-off (MWCO) Amicon filtration units (Millipore, Billerica, Mass.) per the manufacturer's instructions.
  • Desalting and buffer exchange to PBS was performed for SACOL0119 using 10 kDa MWCO Amicon filtration units (Millipore, Billerica, Mass.).
  • Desalting of SACOL0037 into Nano-pure water was achieved using desalting PD-10 columns (GE Healthcare, Waukesha, Wis.) following the manufacturer's procedure.
  • SACOL0037 was lyophilized using a Virtis freezer dryer (SP Scientific, Warminster, Pa.) and the protein particulate was reconstituted in PBS. Protein quantities were determined by BCA (Pierce, Rockland, Ill.) and confirmed by resolving the proteins on 10-20% SDS-PAGE.
  • mice per experimental group were either non-vaccinated with alum adjuvant alone or vaccinated with the quadrivalent biofilm vaccine, the single additional antigen (SA0119), or the combination of all tested antigens (pentavalent vaccine) at 12.5 ⁇ g/antigen in alum adjuvant.
  • Vaccines were administered by intraperitoneal (IP) injection Animals were boosted 14 days later with a non-vaccinated treatment of PBS or vaccinated treatment with the above vaccine compositions suspended in straight PBS.
  • IP intraperitoneal
  • mice 14 days following boost, mice were anesthetized via IP injection of 100 mg/kg ketamine (Ketaset®—Fort Dodge Laboratories, Inc., Fort Dodge, Iowa) and 10 mg/kg xylazine (Rugby Laboratories, Inc., Rockville Center, N.Y.).
  • the left leg of each mouse was cleansed with povidone iodine and rinsed with 70% ethanol before surgical implantation of an sterile 0.25-mm insect pins (Fine Science Tools, Foster City, Calif.) according to the methods previously described (35, 49).
  • 1 ⁇ l of the 1 ⁇ 10 6 CFU/ml S. aureus suspension prepared above was directly inoculated onto the pin implant followed by incision closure. Since 100 CFUs of S.
  • aureus are capable of causing chronic infection in this model (data not shown) and in foreign body infections in humans (19), this infectious dose is at least ten times that required to cause infection. All mice did not undergo any additional treatments after surgery until sacrifice. All animal experiments were performed in accordance to protocols reviewed and approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Maryland School of Medicine (Baltimore, Md.).
  • Bone Cultures In order to demonstrate animal model efficacy, At 4, 7, 14, 21, 28, and 49 days post-implantation, infected and uninfected mice were euthanized, left tibiae were removed, and all soft tissue was dissected from the bone. Using sterile scissors, tibiae were cut into small pieces and placed in 300 ⁇ l of sterile 0.85% saline per 100 ⁇ g of bone. Bones were homogenized using a Polytron PT 1200 handheld homogenizer (Kinematica, Bohemia, N.Y.) and serial 10-fold dilutions of bone homogenates were plated on tryptic soy blood agar plates to enumerate viable S.
  • PNA-FISH Biofilm Detection on Explanted Pins In order to demonstrate biofilms on infected pins in the tibia of mice, the pins from infected and uninfected mice were carefully removed from the tibiae to prevent perturbation of biofilm mass at 7 and 21 days post-implantation. Pins were fixed in 2% paraformaldehyde in PBS before PNA-FISH hybridization with a FITC-labeled S. aureus probe and a rhodamine-labeled universal eukaryotic cell probe, as per manufacturer's instructions (Advandx, Woburn, Mass.).
  • Each pin was then examined with a Zeiss LSM 510 confocal scanning laser microscope (Carl Zeiss, Thomwood, N.Y.) for both green and red fluorescence using a FITC/Texas Red dual-band filter and a 63 ⁇ objective.
  • a Zeiss LSM 510 confocal scanning laser microscope Carl Zeiss, Thomwood, N.Y.
  • S. aureus implant infection results in chronic infection.
  • Tibiae from mice with pins infected with S. aureus and control tibiae with non-infected pins were harvested and processed at 4, 7, 14, 21, 28, and 49 days post-implantation.
  • CFUs were enumerated from homogenized bone to determine the development of chronic infection and bacterial loads in the tibia.
  • Results demonstrate that viable S. aureus were cultured from the S. aureus infected pin and surrounding bone at all time points tested, as far out as 49 days post-infection ( FIG. 1 ).
  • Bacterial loads initially increased to over 3 logs of the infecting dose to >10 8 CFU/tibia but then decreased between 4 and 7 days post-infection.
  • Vaccination with biofilm-upregulated antigens coupled with antibiotic therapy promotes clearance of a S. aureus osteomyelitis infection.
  • Brady et al. identified candidate proteins that were upregulated during the biofilm mode of growth and highly immunogenic in rabbits to formulate a multivalent vaccine against S. aureus biofilm-mediated infections (4).
  • a quadrivalent vaccine composed of SACOL0486, SACOL0688, SACOL0037, and glucosaminidase (10 ⁇ g per recombinant protein) was injected into rabbits at 20 and 10 days prior to challenge using a S. aureus tibial osteomyelitis infection.
  • Vaccinated rabbits had a slight reduction in bacterial load at 14 days post-infection compared to control animals, but bacterial clearance was not achieved (data not shown/Brady 2011). While the quadrivalent vaccine targets the S. aureus biofilm, its components do not activate an effective humoral response against S. aureus planktonic cells and these bacteria persist at day 14 post-infection due to the expression of immunoavoidance factors. Hence, the vaccination strategy was adapted by adding a 10 day vancomycin treatment course starting 14 days post-challenge to eradicate the antibiotic-sensitive, planktonic bacteria dispersed from the biofilm. To evaluate the efficacy of the dual therapy, S. aureus enumeration ( FIG. 2A ) and clearance rates ( FIG.
  • Vaccination with a pentavalent vaccine composed of biofilm-upregulated and planktonic-specific antigens promotes clearance of a S. aureus tibial implant infection.
  • a pentavalent vaccine composed of biofilm-upregulated and planktonic-specific antigens promotes clearance of a S. aureus tibial implant infection.
  • aureus infection was evaluated using a murine tibial implant model, which is a critical evaluation of the vaccine against another biofilm-mediated infection besides osteomyelitis.
  • the pentavalent vaccine which was composed of 12.5 ⁇ g of each recombinant antigen, was administered at 28 and 14 days prior to S. aureus challenge using the tibial implant model.
  • CFUs in the tibiae from mice vaccinated with the pentavalent vaccine were enumerated and compared to counts from mice vaccinated with either the quadrivalent vaccine or monovalent SACOL0119 vaccine and unvaccinated mice. Kidney homogenates were also examined for bacterial counts. We did not observed S.
  • the incorporation of the single planktonic antigen to the multivalent biofilm-directed vaccine enhanced the vaccine efficacy from 50% to 100% prevention of a biofilm-mediated, implant infection in C57BL/6J mice.
  • we achieved complete bacterial clearance of S. aureus which is an accomplishment that has never been attained with other vaccine formulations including those that advanced into clinical trials, using a vaccination strategy that targeted both the planktonic and biofilm phenotypes of the pathogen.

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