USRE31587E - Process for the preparation of 3-iminomethyl derivatives of rifamycin SV - Google Patents
Process for the preparation of 3-iminomethyl derivatives of rifamycin SV Download PDFInfo
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- USRE31587E USRE31587E US06/483,683 US48368383A USRE31587E US RE31587 E USRE31587 E US RE31587E US 48368383 A US48368383 A US 48368383A US RE31587 E USRE31587 E US RE31587E
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D498/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms
- C07D498/02—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms in which the condensed system contains two hetero rings
- C07D498/08—Bridged systems
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D498/00—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms
- C07D498/12—Heterocyclic compounds containing in the condensed system at least one hetero ring having nitrogen and oxygen atoms as the only ring hetero atoms in which the condensed system contains three hetero rings
- C07D498/18—Bridged systems
Definitions
- the intermediate compound obtained from rifamycin S must be subjected to a reduction with ascorbic acid before the trans-imination in order to convert the quinone-like compound into the corresponding hydroquinone-like derivative.
- ascorbic acid may be avoided by carrying out the transimination with at least about 2 molar equivalents of the hydrazine, the second molar equivalent acting as a reducing agent.
- the reaction temperature varies with the solvent employed. If a chlorinated solvent or in general a polar solvent is used, the reaction can be carried out at a relatively low temperature (20°-50° C.), whereas, if a practically apolar solvent such as toluene is employed, it is preferable to operate at a higher temperature.
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Abstract
A process for the preparation in homogeneous phase and in quantitative yield of 3-iminomethyl derivatives of rifamycin SV useful as antibiotic agents. Rifamycin S is reacted in an organic solvent .Iadd.with a compound .Iaddend. ##STR1## to give a solution of .[.1,3-oxazino-(5,6-c) rifamycins.]. .Iadd.an intermediate compound .Iaddend.which is treated with an organic solvent at a pH from 4 to 6. The aqueous phase is discharged and the organic phase is treated with an amine .Iadd.or hydrazine .Iaddend.under basic conditions, the desired derivatives being finally isolated from this organic phase according to usual techniques.
Description
The present invention is concerned with a process for the preparation of 3-iminomethyl derivatives of rifamycin SV useful as antibiotic agents. The French Pat. No. 1,457,435 describes 3-iminomethyl derivatives of rifamycin SV characterized by the following general formula ##STR2## wherein R is oxygen, H(OH), dialkoxy, imino, substituted imino, hydrazono and substituted hydrazono, which are antibiotic substances very active against pathogenic bacteria. A representative compound of this series is the 3-(4-methyl-piperazin-1-yl)iminomethyl-rifamycin SV, a substituted hydrazone derivative of 3-formyl-rifamycin SV known with its International Non-proprietary Name "rifampicin".
According to the above patent, the compounds of formula (I) above are obtained by subjecting a Mannich base of rifamycin SV to an oxidation reaction and hydrolysing the Schiff base thus obtained to the 3-formyl-rifamycin SV (formula (I), R=oxygen).
The latter can be transformed into its acetals (formula (I), R=dialkoxy), reduced to form the corresponding alcohol (formula (I), R=H(OH)) or reacted with primary amines (formula (I), R=substituted imino) or hydrazines (formula (I), R=substituted hydrazono).
French Pat. No. 1,585,041 describes an improved method for the preparation of the 3-hydrazonomethyl derivatives of rifamycin SV of formula (I) above which comprises treating rifamycin S of formula ##STR3## with formaldehyde and a suitable primary amine, preferably tert.butylamine, or with an aldimino derivative obtained from equimolecular amounts of said primary amine and of formaldehyde in the presence of an oxidizing agent, preferably manganese dioxide, then treating the intermediate compound thus obtained with about 2 molar equivalents of the desired hydrazine in order to accomplish both the reduction and the transimination and finally isolating the compound of formula (I) above, in which R represents hydrazono group, by separating the final product from its salt with the primary amine, preferably with tert.butylamine. The preparation of the hydrazonomethyl derivatives starting from rifamycin S according to the French Pat. No. 1,585,041 thus involves a five-steps process comprising a condensation to a Mannich base, an oxidation to a Schiff base, a reduction to the hydroquinone-type compound, a trans-imination and a hydrolysis.
In particular the intermediate compound obtained from rifamycin S must be subjected to a reduction with ascorbic acid before the trans-imination in order to convert the quinone-like compound into the corresponding hydroquinone-like derivative. The use of ascorbic acid may be avoided by carrying out the transimination with at least about 2 molar equivalents of the hydrazine, the second molar equivalent acting as a reducing agent. In any case, there is obtained a salt of the final compound with the employed amine, which precipitates and from which the desired final compound must be isolated by hydrolysis.
The main object of the present invention is to have a process by which it is possible to obtain 3-iminomethyl derivatives of rifamycin SV in quantitative yield, the process being carried out in two steps only and in a very short time and requiring the use of low cost reagents.
Said objects and still other object are obtained by a process which comprises reacting rifamycin S of formula ##STR4## with a compound of formula ##STR5## in which R1 is lower alkyl, lower alkenyl, cycloalkyl of from 5 to 6 carbon atoms, phenyl, benzyl or αor βorβ-phenethyl and R2 is hydrogen or lower alkyl, in a first inert organic solvent at a temperature ranging from 0° C. to the boiling temperature of the solvent employed to give a solution .[.of 1,3-oxazino (5,6-c) rifamycins of formula.]. ##STR6## .Iadd.in the organic solvent of the reaction product of (II) and (V), which reaction product is hereinafter termed the intermediate compound (IV), characterized by the presence of the rifamycin nucleus substituted at the 3-position by carbon which bears nitrogen to which R1 is attached, .Iaddend.in which R1 is as above defined, treating said solution with water and a second inert organic solvent immiscible with water at a pH adjusted at a value from 4 to 6, then discharging the aqueous phase and treating the organic phase with a primary amine, hydrazine, mono-substituted hydrazine or asymmetric di-substituted hydrazine at a temperature from about 20° C. to about 80° C. under basic conditions, 3-iminomethyl derivatives or rifamycin SV of formula ##STR7## being isolated from this solution according to usual techniques.
.Iadd.X in formula (III) is the imino residue of the compound H2 X which is the above mentioned primary amine, hydrazine, mono-substituted hydrazine, or asymmetric di-substituted hydrazine employed to treat the organic phase containing intermediate compound (IV). X is therefore a substituted imino group, a hydrazono group, or a mono-substituted or a di-substituted hydrazono group. .Iaddend.
If the first solvent is mixible with water the compound of formula (IV) precipitates and can be isolated. This .Iadd.intermediate .Iaddend.compound .[.of formula.]. (IV) can be dissolved in an inert organic solvent and treated with a primary amine hydrazine, mono-substituted hydrazine or asymmetric di-substituted hydrazine at a temperature from about 20° C. to about 80° C. under basic conditions, 3-iminomethyl derivatives of rifamycin SV of formula (III) being isolated from this solution according to usual techniques.
The compounds of formula (V) are prepared according to the procedure described in J.Chem.Soc. 123,532, (1923).
The term "lower alkyl", as used herein, includes straight and branched saturated aliphatic hydrocarbon radicals containing from 1 to 6 carbon atoms such as methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, sec.butyl, tert.butyl, n-pentyl, 2-methyl-butyl, n-hexyl, 2-methyl-pentyl. The term "lower alkenyl" includes ethylenically unsaturated aliphatic hydrocarbon radicals containing from 3 to 6 carbon atoms such as allyl, crotyl, methacryl, 3-pentenyl, 3-hexenyl.
The reaction temperature varies with the solvent employed. If a chlorinated solvent or in general a polar solvent is used, the reaction can be carried out at a relatively low temperature (20°-50° C.), whereas, if a practically apolar solvent such as toluene is employed, it is preferable to operate at a higher temperature.
The reaction time depends upon the nature of the starting material employed and upon the temperature. Even though the reaction time can be prolonged up to 24 hours at 40° C. and the reaction mixture can be allowed to stand at about 0° C. for other 24 hours without observing any degradation of the rifamycin molecule, the conversion of the .[.1,3-oxazino(5,6-c)rifamycins of formula.]. .Iadd.intermediate compound .Iaddend.(IV) above into the 3-iminomethyl derivatives of formula (III) is practically immediate in the interval of temperature given above (20°-80° C.). In general, under these conditions the reaction time varies between few minutes and a maximum of 3 hours.
The primary amine, hydrazine, mono-substituted hydrazine or asymmetric di-substituted hydrazine is employed in an amount preferably ranging between about the stoichiometrical amount and an excess of 4-5 moles of the primary amine, hydrazine, mono-substituted hydrazine or asymmetric di-substituted hydrazine per mole of .[.starting.]. .Iadd.intermediate .Iaddend.compound (IV).
Exemplary amines or hydrazines which can react with the .[.1,3-oxazino (5,6-c)rifamycins of formula.]. .Iadd.intermediate compounds .Iaddend.(IV) above to give corresponding 3-iminomethyl derivatives of formula (III) are benzyl-amine, α-or β-phenethylamine, aniline, o-toluidine, m-toluidine, p-toluidine, 2,6-lutidine, p-chloraniline, 2-naphthylamine, 2-aminopyridine, 4-amino-pyridine, hydrazine, benzylhydrazine, phenylhydrazine, 1-amino-pyrrolidine, 1-aminopiperidine, 4-amino-morpholine and 1-amino-4-methyl-piperazine which last, according to the present invention, gives rifampicin.
The basic conditions under which the reaction is carried out are obtained either by using an excess (up to about 4-5 moles) of the primary amine, hydrazine, mono-substituted hydrazine or asymmetric di-substituted hydrazine or by using in the reaction medium a secondary or tertiary amine such as pyrrolidine, trimethylamine, triethylamine, 1-methylpyrrolidine. The method according to which at the end of the reaction the 3-iminomethyl derivatives of formula (III) are isolated preferably includes adjusting the pH of the reaction mixture to slight acid values, washing with water to eliminate the excess of the salified organic base, concentrating the reaction mixture and taking up with a suitable solvent. Thus, the 3-iminomethyl derivatives of rifamycin SV are directly obtained from compound (IV) in practically quantitative yield.
Even though any .[.1,3-oxazino (6,7-c) rifamycin of formula.]. .Iadd.intermediate compound .Iaddend.(IV) above can be converted into the 3-iminomethyl derivatives of formula (III), the best yields and the shortest reaction times are obtained by using an .[.1,3 oxazino (5,6-c)rifamycin of formula.]. .Iadd.intermediate compound .Iaddend.(IV), where R1 is lower alkyl or lower alkenyl. In such a case, after 5-20 minutes the reaction is over and the compounds of formula (III) may be isolated in practically quantitative yield.
In order to make the features of the present invention clear, some examples of said process will now be given:
A mixture of 6.5 g. N,N-dihydroxymethyl-tert.butylamine, 13.9 g. rifamycin S and 40 ml. dimethylformamide is heated at 33° C. under stirring for 60 minutes. The blue solution thus obtained is poured into 300 ml. of water acidified with 3 ml. of acetice acid and extracted with 200 ml. chloroform. To the organic phase there are added 5.5 g. pyrrolidine and 3 g. 1-amino-4-methylpiperazine and the mixture is stirred 40 minutes at 30° C. The reaction mixture is washed with water acidified with acetic acid up to pH 5 and then with water.
The organic phase is dried over sodium sulfate, filtered and concentrated to dryness to give 15.4 g. of a crude product which, by recrystallization from acetone, affords 13.2 g. of chromatografically pure rifampicin.
To a solution of 18 g. of rifamycin S dissolved in 65 ml. of dimethylsulfoxide in a 250 ml. four-necked flask, 10 ml. of di(isobutoxymethyl)-methylamine (formula V, R1 =CH3, R2 =isobutyl) are added and the solution is heated for 5 minutes at 50° C. The blue solution thus obtained is diluted with a mixture of 375 ml. of water acidified with 6 ml. of acetic acid 96%, and extracted with 250 ml. of chloroform. The organic layer is separated and 5.5 g. of pyrrolidine and 4 g. of 1-amino-4-methyl-piperazine are added. The solution is heated 60 minutes at 40° C., then acidified to pH 5 with diluted acetic acid and washed with water. The organic phase is dried over sodium sulfate, filtered and concentrated to dryness. Recrystallization of the residue from acetone affords pure rifampicin in practically quantitative yield.
To a solution of 18 g. rifamycin S in 65 ml. dimethylsulfoxide, 10 ml. diisobutoxymethyl-methylamine are added and the mixture is heated 5 minutes at 50° C., then poured into 375 ml. of distilled water acidified with 6 ml. of 96% acetic acid and extracted with 250 ml. chloroform. The organic phase is separated, treated with 11 g. triethylamine and 4 g. 1-amino-4-methyl-piperazine, then heated 24 hours at 40° C. By operating as described in Example 17, there are obtained 19.2 g. of a rifampicin showing an activity of 98.7%.
A solution of 6.5 g. of N,N dihydroxymethyl-tert.butylamine, 40 ml. of dimethylformamide and 13.9 g. of rifamycin S is stirred in a 100 ml. four-necked flask for 60 minutes at 33° C.
The reaction mixture is poured into 300 ml. of water acidified with 2 ml. of acetic acid. The obtained precipitate is filtered, washed with water and dried at 40° C., under vacuum.
Yield: 14.5 g of .[.N,-tert. butyl-1,3-oxazino (5,6-c) rifamycin..]. .Iadd.intermediate compound having tert. butyl attached to nitrogen. .Iaddend.
A mixture of 2.8 g. of pyrrolidine and 1.38 g. of 1-amino-4-methyl-piperazine is added to a solution of 7.9 g. of .[.N-tert.butyl-1,3-oxazino (5,6-c)rifamycin (.]. .Iadd.the intermediate compound .Iaddend.prepared according to Example 4.[.).]. in 30 ml. of tetrahydrofuran in a 100 ml. four-necked flask. After 20 minutes stirring at room temperature the reaction is over. The reaction mixture is dissolved in 100 ml. chloroform, acidified to pH 5 with diluted acetic acid and washed with water. The chloroformic phase is dried with sodium sulfate and, after filtration, the solvent is evaporated to give 8.2 g. of a crude product which, after recrystallization from acetone yields 7.2 g. of rifampicin as a unitary product on thin layer chromatography.
4 g. of .[.benylamine.]. .Iadd.benzylamine .Iaddend.are added to a solution of 7.9 g. of .[.N-tert.butyl-1,3-oxazino (5,6-c) rifamycin.]. .Iadd.the intermediate compound .Iaddend..[.(.]. prepared according to Example 4.[.).]. in 30 ml. of tetrahydrofuran and after 15 minutes stirring at 30° C. the reaction is complete. The solution is diluted with 100 ml. of chloroform, acidified to pH 5 with diluted acetic acid, washed with water and dried over sodium sulfate. After filtration, the solvent is evaporated and the product is collected. There are obtained 7.8 g. of 3-benzyliminomethyl rifamycin SV (formula III, X═N--CH2 --C6 H5). The U.V. spectrum shows an absorption max. at 555 mμ.
1.5 g. of hydrazine hydrate are added to a solution of 6 g. of .[.N-tert.butyl-1,3-oxazino (5,6-c)rifamycin.]. .Iadd. the intermediate compound .Iaddend..[.(.]. prepared according to Example 4.[.).]. in 30 ml. of tetrahydrofuran. After 5 minutes stirring at room temperature the reaction is complete. By operating as set forth in Example 5, 5.3 g. of the hydrazone of the 3-formyl-rifamycin SV (formula III, X═N--NH2) are obtained.
The U.V. spectrum shows an absorption max. at 325 and 473 mμ.
13.9 g. of rifamycin S are dissolved in 40 ml. of dimethylacetamide, in a 100 ml. four-necked flask.
7 of N,N diisopropoxymethyl-allylamine are added and the solution is heated for 15 minutes at 70° C. The reaction mixture is poured into 300 ml. of water acidified with 2 ml. of acetic acid. The obtained precipitate is filtered, washed with water and dried at 40° C., under vacuum.
Yield: 14.3 g. of .[.N-allyl-1,3-oxazino-(5,6c)rifamycin..]. .Iadd.intermediate compound having allyl attached to nitrogen. .Iaddend.
A mixture of 2.9 g. of 1-methyl-pyrrolidine and 1.38 g. of 1-amino-4-methyl-piperazine is added to a solution of 7.8 g. of .[.N-allyl-1,3-oxazino (5,6-c)rifamycin.]. .Iadd.the intermediate compound .Iaddend..[.(.].prepared according to Example 8.[.).]. in 40 ml. methylene chloride in a 100 ml. four-necked flask.
After 20 minutes stirring the reaction is over and the mixture is dissolved in 100 ml. chloroform, acidified to pH 5 with diluted acetic acid and washed with water. The chloroformic phase is dried over sodium sulfate, filtered, concentrated to dryness and recrystallized from acetone to give 7.2 g. of rifampicin as a pure product. Only one spot on T.L.C.
A mixture of 13.9 g. of rifamycin S, 6.5 g. of N,N dimethoxymethylcyclohexylamine and 4.0 ml. of dimethylsulfoxide is heated in a 100 ml. four-necked flask for 45 minutes at 50° C. The reaction mixture is poured into 300 ml. of water acidified with 2 ml. of acetic acid. The obtained precipitate is filtered, washed with water and dried at 40° C., under vacuum.
Yield: 15.3 of .[.N-cyclohexyl-1,3-oxazino(5,6-c)rifamycin..]. .Iadd.intermediate compound having cyclohexyl attached to nitrogen. .Iaddend.
A solution of 3.1 g. of pyrrolidine in 5 ml. toluene in 10 minutes is added to a mixture of 7.1 g. of .[.N-cyclohexyl-1,3-oxazino(5,6-c)rifamycin.]. .Iadd.the intermediate compound .Iaddend..[.(.].prepared according to Example 10.[.).]. 45 ml. of toluene and 1.2 g. of 1-amino-4-methyl-piperazine at 75° C.
After 10 minutes heating, the reaction mixture is cooled, taken up with 100 ml. chloroform, washed with water acidified with acetic acid and then with water. The dried organic phase is concentrated to dryness and the residue is recrystallized from acetone to give 5.8 g. of pure rifampicin.
13.9 g. of rifamycin S are dissolved in 40 ml. of hexamethylphosphorictriamide in a 100 ml. four-necked flask, 7.5 g. of N,N diethoxymethylbenzylamine are added and the solution is heated for 20 minutes at 60° C. The reaction mixture is poured into 400 ml. of water acidified with 2 ml. of acetic acid. The obtained precipitate is filtered, washed with water and dried at 40° C., under vacuum.
Yield: 15.8 g. of .[.N-benzyl-1,3-oxazino(5,6-c)rifamycin..]. .Iadd.intermediate compound having benzyl attached to nitrogen. .Iaddend.
A mixture of 2.9 g. of 1-methyl-pyrrolidine and 1.38 g. of 1-amino-4-methyl-piperazine is added to a solution of 8.3 g. of .[.N-benzyl-1,3-oxazino (5,6-c)rifamycin.]. .Iadd.the intermediate compound .Iaddend..[.(.].prepared according to Example 12.[.).]. in 40 ml. methylene chloride in a 100 ml. four-necked flask.
After 20 minutes stirring the reaction is over and the mixture is dissolved in 100 ml. chloroform, acidified to pH with 5 with diluted acid and washed with water. The chloroformic phase is dried over sodium sulfate, filtered, concentrated to dryness and recrystallized from acetone to give 7.2 g. of rifampicin as a pure product. Only one spot on T.L.C.
14 g. of rifamycin S are dissolved in 40 ml. acetone in a 100 ml. four-necked flask. 5 g. of N,N-diisobutoxymethyl-methylamine are added and the solution is stirred for 3 hours at 40° C. The reaction mixture is poured into 300 ml. of water acidified with 3 ml. of acetic acid. The obtained precipitate is filtered, washed with water and dried at 40° C., under vacuum.
Yield: 13.8 g. of crude product.
The crude product is mixed with 60 ml. of toluene in a 100 ml. flask; the mixture is stirred for 10 minutes at 60° C. and then filtered.
Yield: 12.5 g. of .[.N -methyl-1,3-oxazine(5,6-c)rifamycin..]. .Iadd.intermediate compound having methyl attached to nitrogen. .Iaddend.
A mixture of 14 g. of rifamycin S, 50 ml. of n-propyl alcohol and 5.5 g. of N,N diisobutoxymethyl-methylamine is stirred in a 100 ml. four-necked flask for 60 minutes at 60° C.
The reaction solution is poured into 500 ml. of water acidified with 5 ml. of acetic acid. The obtained precipitate is filtered, washed with water and dried at 40° C., under vacuum.
Yield: 12.2 g. of crude product.
The crude product is dissolved in 100 ml. of chloroform and a mixture of 250 ml. of toluene and 750 ml. of petroleum ether is added. The obtained precipitate is filtered and dried at 40° C., under vacuum.
Yield: 4.2 g of .[.N-methyl-1,3-oxazino(5,6-c)rifamycin..]. .Iadd.intermediate compound having methyl attached to nitrogen. .Iaddend.
The product is identical to that described in example 14.
A solution of 3.1 g. of pyrrolidine in 5 ml. toluene in 10 minutes is added to a mixture of 6.5 g. of .[.N-methyl-1,3-oxazino(5,6c)rifamycin.]. .Iadd.the intermediate compound .[.(.].prepared according to Examples 14 and 15.[.).]. 45 ml. of toluene and 1.2 g. of 1-amino-4-methyl-piperazine at 75° C.
After 10 minutes heating, the reaction mixture is cooled, taken up with 100 ml. chloroform, washed with water acidified with acetic acid and then with water. The dried organic phase is concentrated to dryness and the residue is recrystallized from acetone to give 5.8 g. of pure rifampicin.
A solution of 7.5 g. of .[.N-methyl-1,3-oxazine(5,6-c)rifamycin.]. .Iadd.the intermediate compound .Iaddend..[.(.]. prepared according to Examples 14 and 15.[.).]. in 50 ml, chloroform is treated with 4 g. triethylamine and 1.5 g. 1-amino-4-methylpiperazine and the mixture thus obtained is allowed to stand 24 hours under stirring. The pH is adjusted to 5 by diluted acetic acid and the solution is washed with water. The organic phase is dried with sodium sulfate, filtered, concentrated to dryness and the residue is taken up with 40 ml. acetone. After standing overnight at 0° C., the precipitate thus obtained is filtered and 7.2 g. of pure rifampicin (activity: 95.5%) are isolated.
Claims (10)
1. A process for the preparation of 3-iminomethyl derivatives of rifamycin SV of formula ##STR8## .Iadd.where X is the imino residue of the compound H2 X which is a primary amine, hydrazine, mono-substituted hydrazine or asymmetric di-substituted hydrazine .Iaddend.which comprises reacting rifamycin S of formula ##STR9## with a compound of formula ##STR10## in which R1 is lower alkyl, lower alkenyl, cycloalkyl having from 5 to 6 carbon atoms, phenyl, benzyl or α- or β-phenethyl and R2 is hydrogen or lower alkyl, in a first inert organic solvent at a temperature ranging from 0° C. to the boiling temperature of the solvent to give a solution .[.of 1,3-oxazino(5,6-c)rifamycins of formula.]. ##STR11## .Iadd.in the first inert organic solvent of the intermediate compound (IV) which is the reaction product of (II) and (V), characterized by the presence of the rifamycin nucleus substituted at the 3-position by carbon which bears nitrogen to which R1 is attached .Iaddend.in which R1 is as above defined, treating said solution .Iadd.of the intermediate compound (IV) .Iaddend.with water and a second inert organic solvent immixible with water at a pH adjusted at a value from 4 to 6, then discharging the aqueous phase and treating the organic phase with a primary amine, hydrazine, mono-substituted hydrazine or asymmetric di-substituted hydrazine at a temperature from about 20° C. to about 80° C. under basic conditions, the derivatives (III) being finally isolated from the organic phase according usual techniques.
2. A process according to claim 1, wherein R1 is t.butyl, methyl or allyl.
3. A process according to claim 1, wherein said first inert organic solvent is a dipolar aprotic solvent.
4. A process according to claim 3, wherein said dipolar aprotic solvent is dimethylsulfoxide, dimethylformamide or dimethylacetamide.
5. A process according to claim 1, wherein said second inert organic solvent are chlorinated or aromatic solvents.
6. A process according to claim 1, wherein said basic conditions are obtained by addition of a secondary or tertiary amine to the organic phase.
7. A process according to claim 6, wherein said secondary or tertiary amines are pyrrolidine, trimethylamine, triethylamine or 1-methyl-pyrrolidine.
8. A process according to claim 1, wherein said derivatives (III) are isolated from said organic phase by acidifying said phase to a pH from about 4 to about 5.5, washing with water, drying the phase and evaporating the solvent.
9. A process according to claim 1, wherein R1 is t.butyl, methyl or allyl, said first inert organic solvent is dimethyl sulfoxide, dimethyl formamide or dimethyl acetamide, the temperature at which the compound of formula (II) is reacted with the compound of formula (V) is between about 15° and 100° C. said second inert organic solvent is methylene chloride or chloroform, said treatment of the organic phase being effected with 1-amino-4-methyl-piperazine substantially at room temperature, said basic conditions being obtained by adding pyrrolidine to the organic phase, the derivative isolated from this organic phase being rifampicin.
10. A process for the preparation of 3-iminomethyl derivatives of rifamycin SV of formula ##STR12## .Iadd., where X is the imino residue of the compound H2 X which is a primary amine, hydrazine, a mono-substituted hydrazine or asymmetric di-substituted hydrazine .Iaddend.which comprises reacting rifamycin S of formula ##STR13## with a compound of formula ##STR14## in which R1 is lower alkyl, lower alkenyl, cycloalkyl having from 5 to 6 carbon atoms, phenyl, benzyl or α- or β-phenethyl and R2 is hydrogen or lower alkyl, in a dipolar solvent at a temperature ranging from 15° C. to 100° C. to give a solution which is treated with water acidified at a pH from about 4 to about 6 to precipitate .[.1,3-oxazino(5,6-c)rifamycins of formula.]. ##STR15## .Iadd.an intermediate compound (IV), the intermediate compound .Iaddend. .[.the 1,3-oxazino(5,6-c)rifamycins.]. thus obtained being dissolved in an inert organic solvent and treated with a primary amine, hydrazine, mono-substituted hydrazine or asymmetric di-substituted hydrazine at a temperature from about 20° C. to about 80° C. under basic conditions, the derivatives (III) being finally isolated from the organic solution according to usual techniques.
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IT27066/73 | 1973-07-25 | ||
IT2706673A IT1043857B (en) | 1973-07-25 | 1973-07-25 | 1,3-Oxazino (5,6-c)-rifamycins - antibiotics and intermediates for 3-iminomethyl derivs such as rifampicin |
IT19774/74 | 1974-01-24 | ||
IT1977474A IT1054154B (en) | 1974-01-24 | 1974-01-24 | 1,3-Oxazino (5,6-c)-rifamycins - antibiotics and intermediates for 3-iminomethyl derivs such as rifampicin |
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US05/472,887 Reissue US3963705A (en) | 1973-07-25 | 1974-05-23 | Process for the preparation of 3-iminomethyl derivatives of rifamycin SV |
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---|---|---|---|
US05/472,887 Expired - Lifetime US3963705A (en) | 1973-07-25 | 1974-05-23 | Process for the preparation of 3-iminomethyl derivatives of rifamycin SV |
US472886A Expired - Lifetime US3925366A (en) | 1973-07-25 | 1974-05-23 | 1,3-Oxazino(5,6-c)rifamycins and method for preparing the same |
US06/483,683 Expired - Lifetime USRE31587E (en) | 1973-07-25 | 1983-04-11 | Process for the preparation of 3-iminomethyl derivatives of rifamycin SV |
Family Applications Before (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US05/472,887 Expired - Lifetime US3963705A (en) | 1973-07-25 | 1974-05-23 | Process for the preparation of 3-iminomethyl derivatives of rifamycin SV |
US472886A Expired - Lifetime US3925366A (en) | 1973-07-25 | 1974-05-23 | 1,3-Oxazino(5,6-c)rifamycins and method for preparing the same |
Country Status (25)
Country | Link |
---|---|
US (3) | US3963705A (en) |
JP (2) | JPS5339440B2 (en) |
AR (2) | AR207762A1 (en) |
AT (2) | AT334530B (en) |
BE (2) | BE815122A (en) |
CA (2) | CA1024139A (en) |
CH (2) | CH615683A5 (en) |
DD (2) | DD112133A5 (en) |
DE (2) | DE2428387A1 (en) |
DK (2) | DK135286B (en) |
ES (2) | ES429059A1 (en) |
FI (2) | FI153574A (en) |
FR (2) | FR2257297B1 (en) |
GB (2) | GB1454801A (en) |
HU (2) | HU168723B (en) |
IE (2) | IE39388B1 (en) |
IN (2) | IN138735B (en) |
NL (2) | NL7409892A (en) |
NO (2) | NO142305C (en) |
PH (1) | PH13691A (en) |
PL (1) | PL91647B1 (en) |
RO (1) | RO63865A (en) |
SE (2) | SE407803B (en) |
SU (2) | SU613725A3 (en) |
YU (2) | YU36720B (en) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AR207762A1 (en) * | 1973-07-25 | 1976-10-29 | Archifar Ind Chim Trentino | PROCEDURE FOR THE PREPARATION OF 3 - ((4-METHYL-1-PIPERAZIN-1-IL) -IMINETHYL-RIFAMYCIN SV OR RIFAMPICIN |
GB1576886A (en) * | 1977-04-20 | 1980-10-15 | Lepetit Spa | Rifamycin derivatives |
US4179439A (en) * | 1977-07-01 | 1979-12-18 | Intreprinderea De Antibiotice Iasi | Rifamycins and method for their preparation |
JPS5473798A (en) * | 1977-11-15 | 1979-06-13 | Kanebo Ltd | Novel 1,3-oxazino (5,6-c) rifamycin derivative |
JPS5473799A (en) * | 1977-11-16 | 1979-06-13 | Kanebo Ltd | Preparation of 1,3-oxazino (5,6-c) rifamycin derivative |
GB1594134A (en) * | 1977-11-25 | 1981-07-30 | Holco Investment Inc | Rifamycins |
JPS5484600A (en) * | 1977-12-15 | 1979-07-05 | Kanebo Ltd | Preparation of 1,3-oxazino 5,6-c rifamycin derivative |
JPS5776597A (en) * | 1980-10-30 | 1982-05-13 | Kiyouritsu Kurieiteibu Asoshie | Electric musical instrument |
JPS58115705U (en) * | 1982-02-01 | 1983-08-08 | 株式会社ニコン | camera viewfinder optical system |
GB8308166D0 (en) * | 1983-03-24 | 1983-05-05 | Erba Farmitalia | Preparation of azinomethyl-rifamycins |
GB8318072D0 (en) * | 1983-07-04 | 1983-08-03 | Lepetit Spa | Water-soluble rifampicin derivatives |
ATE318515T1 (en) * | 1996-11-19 | 2006-03-15 | Univ Georgetown | METHOD FOR INHIBITING HEREGULIN AND ITS RECEPTOR AND USE FOR INHIBITING CANCER CELLS |
WO2009137380A1 (en) * | 2008-05-05 | 2009-11-12 | Janssen Pharmaceutica Nv | 3-hydrazone piperazinyl rifamycin derivatives useful as antimicrobial agents |
CN103755723B (en) * | 2014-02-07 | 2016-04-20 | 天津大学 | A kind of preparation method of rifampicin I crystal form |
CN103951677B (en) * | 2014-03-17 | 2016-04-06 | 四川省长征药业股份有限公司 | The preparation method of rifapentine |
CN106632394A (en) * | 2016-11-15 | 2017-05-10 | 南京工业大学 | Method for preparing rifampicin by using kettle type reaction device and microchannel reaction device for series reaction |
CN106749326A (en) * | 2016-11-28 | 2017-05-31 | 南京工业大学 | Method for continuously producing and preparing rifampicin from rifamycin S sodium salt |
CN111848639A (en) * | 2020-07-09 | 2020-10-30 | 华东理工大学 | Process for synthesizing rifampicin |
KR102688151B1 (en) | 2021-12-28 | 2024-07-24 | 주식회사 종근당바이오 | Novel preparation method for rifampicin with reduced MNP |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3342810A (en) * | 1964-07-31 | 1967-09-19 | Lepetit Spa | Derivatives of rifamycin sv |
US3349082A (en) * | 1964-04-02 | 1967-10-24 | Lepetit Spa | Mannich bases of rifamycin sv |
US3542762A (en) * | 1967-06-07 | 1970-11-24 | Lepetit Spa | Process for rifamycins |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1795568C3 (en) * | 1964-07-31 | 1974-07-18 | Gruppo Lepetit S.P.A., Mailand (Italien) | 3-Hydroxymethyl-rifamycin SV and process for its preparation. Eliminated from: 1595883 |
AR207762A1 (en) * | 1973-07-25 | 1976-10-29 | Archifar Ind Chim Trentino | PROCEDURE FOR THE PREPARATION OF 3 - ((4-METHYL-1-PIPERAZIN-1-IL) -IMINETHYL-RIFAMYCIN SV OR RIFAMPICIN |
-
1974
- 1974-01-01 AR AR254828A patent/AR207762A1/en active
- 1974-01-01 AR AR254829A patent/AR207763A1/en active
- 1974-05-07 SE SE7406073A patent/SE407803B/en not_active IP Right Cessation
- 1974-05-07 SE SE7406074A patent/SE393112B/en not_active IP Right Cessation
- 1974-05-08 FR FR7415837A patent/FR2257297B1/fr not_active Expired
- 1974-05-08 GB GB2025674A patent/GB1454801A/en not_active Expired
- 1974-05-08 GB GB2025774A patent/GB1454802A/en not_active Expired
- 1974-05-09 IN IN1038/CAL/74A patent/IN138735B/en unknown
- 1974-05-09 IN IN1039/CAL/74A patent/IN139074B/en unknown
- 1974-05-14 YU YU1325/74A patent/YU36720B/en unknown
- 1974-05-14 YU YU1324/74A patent/YU36719B/en unknown
- 1974-05-14 IE IE1034/74A patent/IE39388B1/en unknown
- 1974-05-14 IE IE1035/74A patent/IE39389B1/en unknown
- 1974-05-15 NO NO741763A patent/NO142305C/en unknown
- 1974-05-15 NO NO741764A patent/NO741764L/no unknown
- 1974-05-16 BE BE144391A patent/BE815122A/en unknown
- 1974-05-17 FI FI1535/74A patent/FI153574A/fi unknown
- 1974-05-21 RO RO7400078880A patent/RO63865A/en unknown
- 1974-05-23 US US05/472,887 patent/US3963705A/en not_active Expired - Lifetime
- 1974-05-23 US US472886A patent/US3925366A/en not_active Expired - Lifetime
- 1974-05-23 FI FI1575/74A patent/FI60397C/en active
- 1974-05-23 PL PL1974171342A patent/PL91647B1/pl unknown
- 1974-05-24 DK DK284774AA patent/DK135286B/en unknown
- 1974-05-24 CA CA200,755A patent/CA1024139A/en not_active Expired
- 1974-05-24 DK DK284874A patent/DK284874A/en unknown
- 1974-05-31 CA CA201,355A patent/CA1022548A/en not_active Expired
- 1974-06-10 DD DD179054A patent/DD112133A5/xx unknown
- 1974-06-12 DE DE19742428387 patent/DE2428387A1/en active Pending
- 1974-06-17 AT AT496174A patent/AT334530B/en not_active IP Right Cessation
- 1974-06-17 PH PH15952A patent/PH13691A/en unknown
- 1974-07-04 JP JP7592774A patent/JPS5339440B2/ja not_active Expired
- 1974-07-04 JP JP7592874A patent/JPS5314080B2/ja not_active Expired
- 1974-07-05 SU SU2041832A patent/SU613725A3/en active
- 1974-07-09 SU SU742040820A patent/SU576943A3/en active
- 1974-07-10 DE DE2433105A patent/DE2433105A1/en active Granted
- 1974-07-11 DD DD179869A patent/DD112267A5/xx unknown
- 1974-07-17 FR FR7424949A patent/FR2245631B1/fr not_active Expired
- 1974-07-18 BE BE146706A patent/BE817823A/en not_active IP Right Cessation
- 1974-07-19 AT AT596774A patent/AT334531B/en not_active IP Right Cessation
- 1974-07-22 NL NL7409892A patent/NL7409892A/en unknown
- 1974-07-23 NL NLAANVRAGE7409955,A patent/NL181992C/en not_active IP Right Cessation
- 1974-07-24 HU HUAI239A patent/HU168723B/hu unknown
- 1974-07-24 HU HUAI240A patent/HU167983B/hu unknown
- 1974-07-24 CH CH1020074A patent/CH615683A5/de not_active IP Right Cessation
- 1974-07-24 CH CH1019974A patent/CH611296A5/xx not_active IP Right Cessation
- 1974-07-24 ES ES429059A patent/ES429059A1/en not_active Expired
- 1974-07-24 ES ES429060A patent/ES429060A1/en not_active Expired
-
1983
- 1983-04-11 US US06/483,683 patent/USRE31587E/en not_active Expired - Lifetime
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3349082A (en) * | 1964-04-02 | 1967-10-24 | Lepetit Spa | Mannich bases of rifamycin sv |
US3342810A (en) * | 1964-07-31 | 1967-09-19 | Lepetit Spa | Derivatives of rifamycin sv |
US3542762A (en) * | 1967-06-07 | 1970-11-24 | Lepetit Spa | Process for rifamycins |
Non-Patent Citations (4)
Title |
---|
"Chemical Abstracts", vol. 90, 1979, Item 204,159b, abstracting, (Japan) Kokai Tokkyo Koho, 78,147,097, Dec. 21, 1978. |
Chemical Abstracts , vol. 90, 1979, Item 204,159b, abstracting, (Japan) Kokai Tokkyo Koho, 78,147,097, Dec. 21, 1978. * |
Tsukamoto et al., "Chem. Pharm. Bull.", (Japan), vol. 28, No. 8, pp. 2309-2317, (1980). |
Tsukamoto et al., Chem. Pharm. Bull. , (Japan), vol. 28, No. 8, pp. 2309 2317, (1980). * |
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Owner name: GRUPPO LEPETIT S.P.A. Free format text: MERGER;ASSIGNOR:ARCHIFAR-LABORATORI CHIMICO-FARMACOLOGICI S.P.A.;REEL/FRAME:003992/0736 Effective date: 19811123 Owner name: GRUPPO LEPETIT S.P.A.,ITALY Free format text: MERGER;ASSIGNOR:ARCHIFAR-LABORATORI CHIMICO-FARMACOLOGICI S.P.A.;REEL/FRAME:003992/0736 Effective date: 19811123 |