US7547542B2 - Arrangement and method for a cassette for preparation of biological specimens - Google Patents

Arrangement and method for a cassette for preparation of biological specimens Download PDF

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Publication number
US7547542B2
US7547542B2 US10/492,885 US49288504A US7547542B2 US 7547542 B2 US7547542 B2 US 7547542B2 US 49288504 A US49288504 A US 49288504A US 7547542 B2 US7547542 B2 US 7547542B2
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US
United States
Prior art keywords
container
lid
biological specimen
specimen
biasing elements
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Expired - Fee Related, expires
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US10/492,885
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English (en)
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US20050100981A1 (en
Inventor
Bjørn G. Bjørnsen
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Total Biopsy AS
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Total Biopsy AS
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Assigned to TOTAL BIOPSY AS reassignment TOTAL BIOPSY AS ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: BJORNSEN, BJORN G.
Publication of US20050100981A1 publication Critical patent/US20050100981A1/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2873—Cutting or cleaving

Definitions

  • This invention regards a cassette (BioCassette) for use in preparing biological specimens to be analysed in a microscope.
  • the cassette is compatible with known laboratory equipment and allows the use of a simplified method that does not subject the specimens to the relatively large number of manipulations/repacking operations that comes with prior art.
  • one or more biological specimens hereunder designated the specimen
  • the specimen are first placed in one holder, which in turn is placed in a larger container containing formalin.
  • the preparation of the specimen starts by removing it from the formalin container and placing it on a filter paper.
  • the filter paper is folded around the specimen and placed with the specimen in a cassette adapted to the subsequent preparation operations.
  • the cassette is closed and placed in a drying apparatus where the water in the specimen is driven out by means of solvents and pure alcohol in combination with moderate heating and pressure fluctuations.
  • the cassette After drying, the cassette is opened, and the filter paper is removed and opened.
  • the specimen is transferred from the filter paper to a mould in which it is placed at the bottom of the mould. Then it is fixed to the bottom of the mould by a few drops of liquid paraffin wax.
  • the cassette When the paraffin wax has solidified, the cassette is placed over the mould. The remaining mould volume and the cassette are filled with paraffin wax.
  • the mould is removed. With this, the specimen is set in a paraffin moulding projecting down from the cassette.
  • the cassette is then mounted in a cutting machine/slicer where thin slices of wax and specimen are sliced from the downward face of the wax moulding.
  • the object of the invention is to remedy the disadvantages of prior art.
  • the specimen Upon sampling, the specimen is placed directly on the bottom of a container.
  • the inside of the bottom may have a layer of material with the same properties as those explained below in relation to the biasing material.
  • the term porous material refers to a material having open pores.
  • a deformable, preferably flexible resilient biasing material is placed over the specimen.
  • the biasing material is connected to the downward facing side of a lid, where the lid is a complementary fit to the container. Hair-like bows, wool, foam or a spongy material are examples of biasing materials.
  • another form of pliant, resilient structure may be used.
  • the materials in contact with the specimen are porous and/or such that they do not impede the free flow of preparation fluid and wax.
  • the lid is pressed down into the container, where it is locked to the container with sufficient force, e.g. by use of a snap lock, the biasing material pressing the specimen against the bottom of the container so as to prevent the specimen from moving during the further processing.
  • the container, the biasing material, the specimen and the lid are immediately placed in formalin for transport or temporary storage.
  • the lid is provided with plate-like, outwardly projecting portions that in addition to fitting as a holder in known slicers, are also suited for marking of the specimen, e.g. by means of bar codes.
  • the specimen When the specimen arrives at the laboratory, it may be placed in a feed/drying machine without intermediate re-packing, in which machine the moisture in the specimen is removed and where the last operation includes applying a thin layer of wax.
  • the container receives an additional fill of liquid paraffin wax through the perforations in the lid. After the paraffin wax has solidified, the container may be removed. With this, the specimen is set in a wax moulding projecting down from the lid. The lid with the sample is then placed in a slicer that is known per se, and in which thin slices are cut from the underside of the wax moulding.
  • the specimen is placed directly in a container with a lid that may be marked in advance, and remains in the same packing until the specimen is cut into slices.
  • the container may be filled with a sufficient amount of wax to obviate the need for a subsequent fill-up of additional wax, such as described above.
  • FIG. 1 is a perspective view of a container and a lid having downwardly projecting spring bows
  • FIG. 2 is a section I-I through the container and the lid after a biological tissue sample has been placed in the container, but before the lid is pushed down into the container;
  • FIG. 3 shows the section I-I after the lid has been pushed down into the container and the spring bows of the lid abut the tissue sample in a clamping manner
  • FIG. 4 shows the section I-I after the container and the lid have been filled with wax
  • FIG. 5 shows the section I-I after the container has been removed
  • FIG. 6 shows a section where the resilient element of the lid is constituted by a spongy material
  • FIG. 7 shows a section through an alternative embodiment of the device according to the invention.
  • reference number 1 denotes a specimen container.
  • a lid 2 designed to be pushed down into the specimen container 1 is provided with a biasing element 6 on the perforated side 4 facing the specimen container 1 , see FIG. 1 .
  • the biasing element 6 may as an example be in the form of hair-like springs, bows, spirals, wool, foam or a spongy porous material.
  • the specimen container 1 On the inside, along two of its opposite upper side edges 8 and 10 , the specimen container 1 is equipped with inwardly projecting beads 12 that are designed to fit lockingly in two complementary grooves 14 in the vertical side faces 16 and 18 of the lid 2 upon the lid 2 being pushed into the specimen container 1 , see FIG. 3 .
  • the lid 2 has at least one outwardly projecting plate 19 well suited for marking of the specimen.
  • the lid 2 When taking a biological specimen 20 , it is immediately placed in the specimen container 1 , see FIG. 2 . Then the lid 2 , which may be marked in advance, is pushed down into the specimen container 1 until the beads 12 of the container 1 correspond with and are pushed into the grooves 14 of the lid 2 , see FIG. 3 . The biasing element 6 of the lid abuts the specimen 20 in a clamping manner, pressing it against the bottom of the specimen container 1 .
  • the specimen container 1 and the lid 2 are filled with liquid wax 24 , see FIG. 4 .
  • the specimen container 1 is filled through the perforations 22 in the lid 2 .
  • the specimen container 1 is removed after the wax 24 has solidified, see FIG. 5 , whereby the specimen is ready to be sliced.
  • the resilient element may be constituted by a spongy porous body 26 designed to be filled with wax.
  • the container 1 and the lid 2 are cylindrical and placed in a separate holder 28 , where the holder 28 serves as a holder in the slicer.
  • a porous material 30 which may also be deformable, is arranged underneath the specimen 20 .

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
US10/492,885 2001-10-19 2002-10-10 Arrangement and method for a cassette for preparation of biological specimens Expired - Fee Related US7547542B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
NO20015130A NO315063B1 (no) 2001-10-19 2001-10-19 Anordning og fremgangsmåte ved kassett for preparering av biologiske pröver
NO20015130 2001-10-19
PCT/NO2002/000366 WO2003034033A1 (fr) 2001-10-19 2002-10-10 Agencement et procede pour une cassette permettant de preparer des echantillons biologiques

Publications (2)

Publication Number Publication Date
US20050100981A1 US20050100981A1 (en) 2005-05-12
US7547542B2 true US7547542B2 (en) 2009-06-16

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
US10/492,885 Expired - Fee Related US7547542B2 (en) 2001-10-19 2002-10-10 Arrangement and method for a cassette for preparation of biological specimens

Country Status (6)

Country Link
US (1) US7547542B2 (fr)
EP (1) EP1436587A1 (fr)
JP (1) JP4095028B2 (fr)
CA (1) CA2462984C (fr)
NO (1) NO315063B1 (fr)
WO (1) WO2003034033A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080299605A1 (en) * 2007-06-01 2008-12-04 Lary Todd P Useful specimen transport apparatus with integral capability to allow three dimensional x-ray images
US9546937B2 (en) 2012-01-26 2017-01-17 Richard-Allan Scientific Company Tissue cassette assembly

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4105164B2 (ja) 2002-09-26 2008-06-25 バイオパス・オートメーション・エル・エル・シー 組織標本のハンドリングおよび包埋を自動化する機器および方法
US7179424B2 (en) * 2002-09-26 2007-02-20 Biopath Automation, L.L.C. Cassette for handling and holding tissue samples during processing, embedding and microtome procedures, and methods therefor
EP1545775B1 (fr) 2002-09-26 2010-06-16 BioPath Automation, L.L.C. Cassette et ensemble d'incorporation, dispositifs de stadification, et procedes de manipulation d'echantillons de tissu
JP4667061B2 (ja) * 2005-02-14 2011-04-06 村角工業株式会社 医療検査用検体薄片の製造方法
US20070116612A1 (en) * 2005-11-02 2007-05-24 Biopath Automation, L.L.C. Prefix tissue cassette
EP1967836B1 (fr) * 2005-12-27 2018-09-12 Kyoto University Cassette pour fixer, enrober et découper un tissu biologique et procédé pour sa manipulation
JP4797167B2 (ja) * 2006-01-18 2011-10-19 国立大学法人 東京大学 包埋用カセット
US20070253870A1 (en) * 2006-05-01 2007-11-01 Operon Biotechnologies, Inc. Specimen tube holder and shipping container
US8383067B2 (en) * 2006-12-12 2013-02-26 Biopath Automation, L.L.C. Biopsy support with sectionable resilient cellular material
JP5112705B2 (ja) * 2007-01-25 2013-01-09 村角工業株式会社 病理組織検査標本作成用の包埋トレイ
JP2009156791A (ja) * 2007-12-27 2009-07-16 Kankyo Kiki:Kk ホルマリン吸収用シート
BRPI1006958B1 (pt) 2009-01-22 2019-11-19 Biopath Automation Llc dispositivos de suporte e orientação de amostras de tecido histológico e métodos de preparação de uma ou mais amostras alongadas de tecido de biópsia para exame histológico
US8308127B1 (en) * 2010-06-07 2012-11-13 Harris Ryan M Faux ice tray system
GB2485240A (en) * 2010-11-08 2012-05-09 Thermo Shandon Ltd Waste liquid wax tray
CN105784457B (zh) * 2016-03-21 2018-04-20 中国医科大学附属第一医院 低温组织样本包埋储存系统

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2878848A (en) * 1953-07-27 1959-03-24 Republic Molding Corp Containers
GB1230913A (fr) 1968-05-01 1971-05-05
US3733768A (en) 1971-02-12 1973-05-22 B Carls Storage of histology specimens
US3982862A (en) 1975-04-03 1976-09-28 Pickett John E P Two-part composite device for histologic tissue processing and embedding
US4569647A (en) * 1983-09-16 1986-02-11 Pelam, Inc. Apparatus for preparing and embedding tissue samples for histological examination
GB2189596A (en) 1986-04-16 1987-10-28 Pa Consulting Services Embedding tissue specimens for sectioning
US4715545A (en) * 1986-02-13 1987-12-29 Sage Products, Inc. Tissue grinding and transport system and method
JPH0363841A (ja) 1989-08-02 1991-03-19 Shizuoka Nippon Denki Software Kk 分散集中管理システムでの障害の監視方式
US5129517A (en) * 1988-11-03 1992-07-14 Oscar Mayer Foods Corporation Food package containing a reclosable means
US5269671A (en) * 1992-02-11 1993-12-14 Mccormick James B Apparatus for embedding tissue samples
EP0807807A1 (fr) 1995-02-03 1997-11-19 Teiji Takezaki Methode de support fixe d'echantillon biopsique, agent de support fixe et cassette d'inclusion
EP0856729A2 (fr) 1997-01-30 1998-08-05 Shandon Scientific Limited Appareil et méthode pour le traitement de tissus
US5817032A (en) 1996-05-14 1998-10-06 Biopath Automation Llc. Means and method for harvesting and handling tissue samples for biopsy analysis

Patent Citations (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2878848A (en) * 1953-07-27 1959-03-24 Republic Molding Corp Containers
GB1230913A (fr) 1968-05-01 1971-05-05
US3733768A (en) 1971-02-12 1973-05-22 B Carls Storage of histology specimens
US3982862A (en) 1975-04-03 1976-09-28 Pickett John E P Two-part composite device for histologic tissue processing and embedding
US4569647A (en) * 1983-09-16 1986-02-11 Pelam, Inc. Apparatus for preparing and embedding tissue samples for histological examination
US4715545A (en) * 1986-02-13 1987-12-29 Sage Products, Inc. Tissue grinding and transport system and method
GB2189596A (en) 1986-04-16 1987-10-28 Pa Consulting Services Embedding tissue specimens for sectioning
US4801553A (en) * 1986-04-16 1989-01-31 Stephen Owen Methods of and apparatus for preparing tissue specimens
US5129517A (en) * 1988-11-03 1992-07-14 Oscar Mayer Foods Corporation Food package containing a reclosable means
JPH0363841A (ja) 1989-08-02 1991-03-19 Shizuoka Nippon Denki Software Kk 分散集中管理システムでの障害の監視方式
US5269671A (en) * 1992-02-11 1993-12-14 Mccormick James B Apparatus for embedding tissue samples
EP0807807A1 (fr) 1995-02-03 1997-11-19 Teiji Takezaki Methode de support fixe d'echantillon biopsique, agent de support fixe et cassette d'inclusion
US5817032A (en) 1996-05-14 1998-10-06 Biopath Automation Llc. Means and method for harvesting and handling tissue samples for biopsy analysis
EP0856729A2 (fr) 1997-01-30 1998-08-05 Shandon Scientific Limited Appareil et méthode pour le traitement de tissus
US5843700A (en) * 1997-01-30 1998-12-01 Shandon Scientific Limited Tissue processing apparatus and method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080299605A1 (en) * 2007-06-01 2008-12-04 Lary Todd P Useful specimen transport apparatus with integral capability to allow three dimensional x-ray images
US7951345B2 (en) * 2007-06-01 2011-05-31 Lary Research & Development, Llc Useful specimen transport apparatus with integral capability to allow three dimensional x-ray images
US9546937B2 (en) 2012-01-26 2017-01-17 Richard-Allan Scientific Company Tissue cassette assembly

Also Published As

Publication number Publication date
EP1436587A1 (fr) 2004-07-14
WO2003034033A1 (fr) 2003-04-24
US20050100981A1 (en) 2005-05-12
CA2462984A1 (fr) 2003-04-24
JP4095028B2 (ja) 2008-06-04
JP2005505776A (ja) 2005-02-24
NO315063B1 (no) 2003-06-30
NO20015130D0 (no) 2001-10-19
CA2462984C (fr) 2013-01-29
NO20015130L (no) 2003-04-22

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