US5322687A - Bacillus thuringiensis cryet4 and cryet5 toxin genes and proteins toxic to lepidopteran insects - Google Patents

Bacillus thuringiensis cryet4 and cryet5 toxin genes and proteins toxic to lepidopteran insects Download PDF

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Publication number
US5322687A
US5322687A US08/100,709 US10070993A US5322687A US 5322687 A US5322687 A US 5322687A US 10070993 A US10070993 A US 10070993A US 5322687 A US5322687 A US 5322687A
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Prior art keywords
gene
seq
cryet4
cryet5
protein
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William P. Donovan
Yuping Tan
Christine S. Jany
Jose M. Gonzalez, Jr.
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Monsanto Co
Monsanto Technology LLC
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Ecogen Inc
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Priority to US08/100,709 priority Critical patent/US5322687A/en
Assigned to ECOGEN INC. A CORP. OF DELAWARE reassignment ECOGEN INC. A CORP. OF DELAWARE ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: DONOVAN, WILLIAM P., GONZALEZ, JOSE M., JR., JANY, CHRISTINE S., TAN, YUPING
Application filed by Ecogen Inc filed Critical Ecogen Inc
Priority to US08/176,865 priority patent/US5616319A/en
Publication of US5322687A publication Critical patent/US5322687A/en
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Priority to AU76298/94A priority patent/AU7629894A/en
Priority to CA002145859A priority patent/CA2145859C/fr
Priority to PCT/US1994/008640 priority patent/WO1995004146A2/fr
Priority to US08/474,038 priority patent/US5679343A/en
Assigned to MONSANTO COMPANY reassignment MONSANTO COMPANY ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: ECOGEN INC., ECOGEN-BIO INC.
Assigned to MONSANTO COMPANY reassignment MONSANTO COMPANY ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: ECOGEN INC., ECOGEN-BIO INC.
Priority to US08/779,046 priority patent/US5854053A/en
Priority to US08/881,340 priority patent/US5942658A/en
Assigned to MONSANTO TECHNOLOGY LLC reassignment MONSANTO TECHNOLOGY LLC ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: PHARMACIA CORPORATION, FORMERLY KNOWN AS MONSATO COMPANY
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal protein (delta-endotoxin)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/075—Bacillus thuringiensis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
US08/100,709 1993-07-29 1993-07-29 Bacillus thuringiensis cryet4 and cryet5 toxin genes and proteins toxic to lepidopteran insects Expired - Lifetime US5322687A (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
US08/100,709 US5322687A (en) 1993-07-29 1993-07-29 Bacillus thuringiensis cryet4 and cryet5 toxin genes and proteins toxic to lepidopteran insects
US08/176,865 US5616319A (en) 1993-07-29 1993-12-30 Bacillus thuringiensis cryET5 gene and related plasmids, bacteria and insecticides
AU76298/94A AU7629894A (en) 1993-07-29 1994-07-28 (bacillus thuringiensis cryet4) and (cryet5) toxin genes and proteins toxic to lepidopteran insects
CA002145859A CA2145859C (fr) 1993-07-29 1994-07-28 Genes cryet4 et cryet5 des toxines de bacillus thuringiensis et a proteines toxiques a l'egard des lepidopteres
PCT/US1994/008640 WO1995004146A2 (fr) 1993-07-29 1994-07-28 Genes et proteines de toxine cryet4 et cryet5 du bacillus thuringiensis, a pouvoir toxique contre les lepidopteres
US08/474,038 US5679343A (en) 1993-07-29 1995-06-07 Bacillus thuringiensis cryET4 and cryET5 protein insecticidal composition and method of use
US08/779,046 US5854053A (en) 1993-07-29 1997-01-06 Bacillus thuringiensis bacteria
US08/881,340 US5942658A (en) 1993-07-29 1997-06-24 Transformed plant with Bacillus thuringiensis toxin gene

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/100,709 US5322687A (en) 1993-07-29 1993-07-29 Bacillus thuringiensis cryet4 and cryet5 toxin genes and proteins toxic to lepidopteran insects

Related Child Applications (1)

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US08/176,865 Division US5616319A (en) 1993-07-29 1993-12-30 Bacillus thuringiensis cryET5 gene and related plasmids, bacteria and insecticides

Publications (1)

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US5322687A true US5322687A (en) 1994-06-21

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Application Number Title Priority Date Filing Date
US08/100,709 Expired - Lifetime US5322687A (en) 1993-07-29 1993-07-29 Bacillus thuringiensis cryet4 and cryet5 toxin genes and proteins toxic to lepidopteran insects
US08/176,865 Expired - Lifetime US5616319A (en) 1993-07-29 1993-12-30 Bacillus thuringiensis cryET5 gene and related plasmids, bacteria and insecticides
US08/474,038 Expired - Lifetime US5679343A (en) 1993-07-29 1995-06-07 Bacillus thuringiensis cryET4 and cryET5 protein insecticidal composition and method of use
US08/779,046 Expired - Lifetime US5854053A (en) 1993-07-29 1997-01-06 Bacillus thuringiensis bacteria
US08/881,340 Expired - Lifetime US5942658A (en) 1993-07-29 1997-06-24 Transformed plant with Bacillus thuringiensis toxin gene

Family Applications After (4)

Application Number Title Priority Date Filing Date
US08/176,865 Expired - Lifetime US5616319A (en) 1993-07-29 1993-12-30 Bacillus thuringiensis cryET5 gene and related plasmids, bacteria and insecticides
US08/474,038 Expired - Lifetime US5679343A (en) 1993-07-29 1995-06-07 Bacillus thuringiensis cryET4 and cryET5 protein insecticidal composition and method of use
US08/779,046 Expired - Lifetime US5854053A (en) 1993-07-29 1997-01-06 Bacillus thuringiensis bacteria
US08/881,340 Expired - Lifetime US5942658A (en) 1993-07-29 1997-06-24 Transformed plant with Bacillus thuringiensis toxin gene

Country Status (4)

Country Link
US (5) US5322687A (fr)
AU (1) AU7629894A (fr)
CA (1) CA2145859C (fr)
WO (1) WO1995004146A2 (fr)

Cited By (19)

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WO1995004146A2 (fr) * 1993-07-29 1995-02-09 Ecogen Inc. Genes et proteines de toxine cryet4 et cryet5 du bacillus thuringiensis, a pouvoir toxique contre les lepidopteres
US5723758A (en) * 1991-09-13 1998-03-03 Mycogen Corporation Bacillus thuringiensis genes encoding lepidopteran-active toxins
US5747025A (en) * 1991-05-14 1998-05-05 Agricultural Genetics Co., Ltd. Biological control of pests
US5759538A (en) * 1995-03-31 1998-06-02 Monsanto Company Bacillus thuringiensis apr and npr genes, apr and npr B.t. strains, and method of use
US5914318A (en) * 1996-11-27 1999-06-22 Ecogen, Inc. Transgenic plants expressing lepidopteran-active δ-endotoxins
US5965428A (en) * 1996-10-08 1999-10-12 Ecogen, Inc. Chimeric lepidopteran-toxic crystal proteins
US6043415A (en) * 1996-10-07 2000-03-28 Ramot Univ. Auth. For Applied Research And Industrial Development Ltd. Synthetic Bacillus thuringiensis cryic gene encoding insect toxin
WO2000073324A2 (fr) * 1999-06-01 2000-12-07 Digital Gene Technologies Inc. Modulation d'expression genique dans une inflammation gastro-intestinale
WO2004020636A1 (fr) * 2002-08-29 2004-03-11 Monsanto Technology, Llc Sequences nucleotidiques codant pour des proteines cry1bb d'expression accrue chez des plantes
WO2007027776A2 (fr) 2005-08-31 2007-03-08 Monsanto Technology Llc Compositions insecticides et procedes de preparation de vegetaux transgeniques resistant aux insectes
EP2292759A1 (fr) 1998-11-04 2011-03-09 Monsanto Technology LLC Procédés servant à transformer des plantes pour exprimer les delta-endotoxines du Bacille thuringiensis
US20110112013A1 (en) * 2009-11-12 2011-05-12 Pioneer Hi-Bred International, Inc. Novel Bacillus thuringiensis Gene with Lepidopteran Activity
WO2016061208A1 (fr) * 2014-10-15 2016-04-21 Pioneer Hi-Bred International Inc Polypeptides insecticides à large spectre d'activité et leurs utilisations
US9567381B2 (en) 2012-03-09 2017-02-14 Vestaron Corporation Toxic peptide production, peptide expression in plants and combinations of cysteine rich peptides
WO2018128744A1 (fr) * 2017-01-04 2018-07-12 Syngenta Participations Ag Compositions et procédés de lutte contre les phytoravageurs
US11267849B2 (en) 2014-10-16 2022-03-08 Monsanto Technology Llc Chimeric insecticidal proteins toxic or inhibitory to lepidopteran pests
US11286284B2 (en) * 2014-10-16 2022-03-29 Monsanto Technology Llc Chimeric insecticidal proteins toxic or inhibitory to Lepidopteran pests
US11447531B2 (en) 2016-10-21 2022-09-20 Vestaron Corporation Cleavable peptides and insecticidal and nematicidal proteins comprising same
US11692016B2 (en) 2012-03-09 2023-07-04 Vestaron Corporation High gene expression yeast strain

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US5807398A (en) * 1995-04-28 1998-09-15 Shaknovich; Alexander Shuttle stent delivery catheter
ATE421973T1 (de) 1999-12-28 2009-02-15 Bayer Bioscience Nv Inzektizide proteinen von bacillus thuringiensis
US6531283B1 (en) 2000-06-20 2003-03-11 Molecular Staging, Inc. Protein expression profiling
KR100432140B1 (ko) * 2001-06-28 2004-05-17 제연호 토양으로부터 분리된 바실러스 투린지엔시스 케이-1 균주 및 이 균주를 이용한 미생물 살충제
JP2004106272A (ja) * 2002-09-17 2004-04-08 Toshiba Mach Co Ltd 射出成形機における異常検知方法
EP2868749B1 (fr) 2004-01-20 2017-10-04 Monsanto Technology LLC Promoteurs chimériques pour une utilisation dans les plantes
US7838729B2 (en) 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
US8609936B2 (en) 2007-04-27 2013-12-17 Monsanto Technology Llc Hemipteran-and coleopteran active toxin proteins from Bacillus thuringiensis
US8580942B2 (en) 2008-04-07 2013-11-12 Monsanto Technology Llc Plant regulatory elements from a metallothionein-like gene and uses thereof
AR073237A1 (es) 2008-08-29 2010-10-20 Monsanto Technology Llc Proteinas de bacillus thuringiensis que son toxinas activas contra hemipteros y coleopteros
US8692065B2 (en) 2009-01-23 2014-04-08 Pioneer Hi Bred International Inc Bacillus thuringiensis gene with lepidopteran activity
WO2010099431A2 (fr) 2009-02-27 2010-09-02 Monsanto Technology Llc Appareil pour culture hydroponique et ses procédés d'utilisation
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EA031636B1 (ru) 2011-05-13 2019-01-31 Монсанто Текнолоджи Ллс Регуляторные элементы растений и их применение
EP2820136B1 (fr) 2012-02-29 2018-03-28 Dow AgroSciences LLC Activateur du virus bacilliforme de la canne à sucre (scbv) et son utilisation en génomique végétale fonctionnelle
EP2834266B1 (fr) 2012-04-06 2019-06-12 Monsanto Technology LLC Protéines toxiques pour des espèces d'insectes hémiptères
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CA2881787A1 (fr) 2012-08-13 2014-02-20 University Of Georgia Research Foundation, Inc. Compositions et procedes d'amelioration de la resistance des plantes contre les parasites
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EP0358557A2 (fr) * 1988-09-06 1990-03-14 Plant Genetic Systems, N.V. Plantes transformées par une séquence d'ADN, lethale pour les lepidoptera, de bacillus thuringiensis
EP0367474A1 (fr) * 1988-11-01 1990-05-09 Mycogen Corporation Souche de bacillus thuringiensis appelée b.t. ps81gg, active contre les lépidoptères nuisibles et gène codant une toxine active contre les lépidoptères.
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