US3972146A - Process for propagating strawberry plants - Google Patents

Process for propagating strawberry plants Download PDF

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Publication number
US3972146A
US3972146A US05/596,908 US59690875A US3972146A US 3972146 A US3972146 A US 3972146A US 59690875 A US59690875 A US 59690875A US 3972146 A US3972146 A US 3972146A
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US
United States
Prior art keywords
shoots
aminopurine
cytokinin
benzyl
nutrient medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US05/596,908
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English (en)
Inventor
Philippe Boxus
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Station des Cultures Fruitieres et Maraicheres
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Station des Cultures Fruitieres et Maraicheres
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Station des Cultures Fruitieres et Maraicheres filed Critical Station des Cultures Fruitieres et Maraicheres
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Publication of US3972146A publication Critical patent/US3972146A/en
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Expired - Lifetime legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques

Definitions

  • the present invention relates to the asexual propagation of plants, and particularly of strawberry plants.
  • axillary or adventive shoot When an axillary or adventive shoot is placed in a nutrient medium containing cytokinin, axillary or adventive shoots are propagated and this continues for as long as the cytokinin remains active, usually about six to eight weeks.
  • the new shoots thus obtained are separted and individualized and can then in turn propagate if they are transplanted into a medium enriched with cytokinin.
  • the propagation of the shoots is stopped when desired by eliminating the cytokinin. Thereafter, the shoots act as young strawberry plants forming leaves and roots which can be planted in soil where they function in the same way as natural runners.
  • a plantlet which had been raised in a sterile manner and obtained from meristems or by some other method was acquired to serve as the "starting material.” It was aseptically transplanted into 25 ⁇ 150 mm test tubes containing 15 ml of a nutrient solution containing the macro-elements of a Knop mineral solution, the micro-elements of Murashige and Skoog, 0.5 mg/l of nicotinic acid, 0.5 mg/l of HCl pyridoxine, 2.0 mg/l of glycine, 0.1 mg/l of HCl thiamine, 100 mg/l of mesoinositol, 1.0 mg/l of indolyl butyric acid, 40.0 g/l of glucose and 8.0 g/l of agar.
  • the nutrient solutions were poured into tubes or jars and sterilized in an autoclave at 110° C for 15 minutes. Other temperatures and times may be chosen for such sterilization as is conventional.
  • the plantlet After spending 3 - 4 weeks in the sterile nutrient medium, the plantlet started to shoot by development of axillary or adventive shoots. This propagation of adventive or axillary shoots continued for at least 3 - 4 weeks. By then, the plantlet had formed a mass of shoots (15 - 30 and sometimes more) which only developed a few leaves and roots. This mass of shoots was separated to individualize each shoot in the sterile medium, and the separated shoots were transplanted each into a fresh nutrient medium.
  • the separated shoots develop leaves and roots after about 3 weeks.
  • the plantlets can be transplanted into a relatively light compost soil which is preferably not sterile in order to avoid surface mildew.
  • the transplanted plantlets easily start growing again provided that a humid atmosphere is maintained (mist spray or relatively tightly sealed transparent plastic tanks).
  • the transplanted plantlets have the vigor and size of a normal runner.
  • the growing room used in connection with the aforedescribed accelerated propagation was held at a constant temperature of 24°C with artificial lighting (6 40W TL cubes per 120 m 2 of shelf) during 16 hours daily.
  • the relative humidity of the air is unimportant.
  • the temperature may be chosen between 10 and 30°C and either be kept constant or varied between night and day.
  • Light can be supplied by any type of lamp provided that a minimum threshold intensity of 1,500 - 2,000 lux is maintained. It is also possible to work in a greenhouse, but a back-up lighting system is necessary if the shelves are superimposed in order to illuminate the lower shelves.
  • Culturing takes place in test tubes or in sterilizable jars. Jars having a capacity between one half and three quarters of a liter have proved particularly suitable for obtaining a large number of plants in a small volume of nutrient medium.
  • Propagation under sterile conditions ensures that the material produced is 100% healthy.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
US05/596,908 1974-07-19 1975-07-17 Process for propagating strawberry plants Expired - Lifetime US3972146A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BE146738A BE817857A (fr) 1974-07-19 1974-07-19 Procede de multiplication acceleree de plants de fraisiers par "micropropagation in vitro" et plantules obtenues.
BE146738 1974-07-19

Publications (1)

Publication Number Publication Date
US3972146A true US3972146A (en) 1976-08-03

Family

ID=3842685

Family Applications (1)

Application Number Title Priority Date Filing Date
US05/596,908 Expired - Lifetime US3972146A (en) 1974-07-19 1975-07-17 Process for propagating strawberry plants

Country Status (9)

Country Link
US (1) US3972146A (en:Method)
AT (1) AT341552B (en:Method)
BE (1) BE817857A (en:Method)
CH (1) CH608942A5 (en:Method)
DE (1) DE2526236A1 (en:Method)
ES (1) ES439198A1 (en:Method)
FR (1) FR2278267A1 (en:Method)
IT (1) IT1031372B (en:Method)
NL (1) NL7508646A (en:Method)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4152869A (en) * 1976-07-16 1979-05-08 National Seed Development Organisation Limited Propagating woody plant material
WO1981003255A1 (en) * 1980-05-12 1981-11-26 Univ Minnesota Aseptic serial propagation of above ground portion of plants
US4326358A (en) * 1980-07-17 1982-04-27 Agrigenetics Research Associates Limited Hybrids
US4381624A (en) * 1980-07-17 1983-05-03 Agrigenetics Research Associates Limited High purity hybrid cabbage seed production
EP0098234A3 (en) * 1982-06-28 1985-07-10 Gyogynoveny Kutato Intezet Process for the production of multiplicative botanical substances
GB2169483A (en) * 1985-01-14 1986-07-16 Nat Res Dev Growing strawberry plants for fruiting out of season
WO2020047209A1 (en) * 2018-08-29 2020-03-05 Clemson University Research Foundation Plant tissue culture devices and methods of culturing and harvesting plant shoot tips

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2364611A1 (fr) * 1976-09-21 1978-04-14 Anvar Procede de multiplication vegetative de vegetaux et plants ainsi obtenus
FR2548523B1 (fr) * 1983-07-06 1986-07-04 Paupardin Colette Procede d'obtention de genepi ayant une teneur elevee en essences aromatiques
ES2040174B1 (es) * 1992-02-24 1994-05-01 Consejo Superior Investigacion Metodo para el enraizamiento de estaquillas semileñosas de chirimoyo (anona cherimola mill.) cv. fino de jete, micropropagadas por cultivo de tejidos in vitro.
MD57Z (ro) * 2009-03-03 2010-03-31 Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы Procedeu de micropropagare a Stevia rebaudiana Bertoni in vitro

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3514900A (en) * 1963-12-06 1970-06-02 Everest Mcdade Method for rapidly reproducing orchids
US3683550A (en) * 1970-04-01 1972-08-15 Del Monte Corp Plant culture method and product
US3816960A (en) * 1972-03-13 1974-06-18 British Petroleum Co Method for growing plants
US3821864A (en) * 1973-04-06 1974-07-02 Scott & Sons Co O Vegetative reproduction of grasses
GB1387821A (en) * 1971-05-18 1975-03-19 American Cyanamid Co Producing chemical plant metabolites by suspension culture

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3514900A (en) * 1963-12-06 1970-06-02 Everest Mcdade Method for rapidly reproducing orchids
US3683550A (en) * 1970-04-01 1972-08-15 Del Monte Corp Plant culture method and product
GB1387821A (en) * 1971-05-18 1975-03-19 American Cyanamid Co Producing chemical plant metabolites by suspension culture
US3816960A (en) * 1972-03-13 1974-06-18 British Petroleum Co Method for growing plants
US3821864A (en) * 1973-04-06 1974-07-02 Scott & Sons Co O Vegetative reproduction of grasses

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4152869A (en) * 1976-07-16 1979-05-08 National Seed Development Organisation Limited Propagating woody plant material
WO1981003255A1 (en) * 1980-05-12 1981-11-26 Univ Minnesota Aseptic serial propagation of above ground portion of plants
US4326358A (en) * 1980-07-17 1982-04-27 Agrigenetics Research Associates Limited Hybrids
US4381624A (en) * 1980-07-17 1983-05-03 Agrigenetics Research Associates Limited High purity hybrid cabbage seed production
EP0098234A3 (en) * 1982-06-28 1985-07-10 Gyogynoveny Kutato Intezet Process for the production of multiplicative botanical substances
GB2169483A (en) * 1985-01-14 1986-07-16 Nat Res Dev Growing strawberry plants for fruiting out of season
WO2020047209A1 (en) * 2018-08-29 2020-03-05 Clemson University Research Foundation Plant tissue culture devices and methods of culturing and harvesting plant shoot tips

Also Published As

Publication number Publication date
ES439198A1 (es) 1977-05-16
AT341552B (de) 1978-02-10
FR2278267B1 (en:Method) 1980-01-11
BE817857A (fr) 1974-11-18
ATA494775A (de) 1977-06-15
IT1031372B (it) 1979-04-30
CH608942A5 (en:Method) 1979-02-15
DE2526236A1 (de) 1976-01-29
NL7508646A (nl) 1976-01-21
FR2278267A1 (fr) 1976-02-13

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