US3877634A - Cell washing centrifuge apparatus and system - Google Patents

Cell washing centrifuge apparatus and system Download PDF

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Publication number
US3877634A
US3877634A US364150A US36415073A US3877634A US 3877634 A US3877634 A US 3877634A US 364150 A US364150 A US 364150A US 36415073 A US36415073 A US 36415073A US 3877634 A US3877634 A US 3877634A
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United States
Prior art keywords
centrifuge
tubes
distributor
fluid
tube
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Expired - Lifetime
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US364150A
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English (en)
Inventor
Vernon C Rohde
Josef Blum
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EIDP Inc
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EI Du Pont de Nemours and Co
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Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Priority to US364150A priority Critical patent/US3877634A/en
Priority to GB2315674A priority patent/GB1470806A/en
Priority to JP5867674A priority patent/JPS5710776B2/ja
Priority to CA200,830A priority patent/CA1007213A/en
Priority to DE19742425165 priority patent/DE2425165A1/de
Application granted granted Critical
Publication of US3877634A publication Critical patent/US3877634A/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B11/00—Feeding, charging, or discharging bowls
    • B04B11/06—Arrangement of distributors or collectors in centrifuges
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B13/00—Control arrangements specially designed for centrifuges; Programme control of centrifuges
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • B04B5/0421—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes pivotably mounted
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification

Definitions

  • the present invention relates toan apparatus and system for treating and washing biological materials and. more particularlyi to a system including means for centrifuging said materials in a plurality of swinging centrifuge tubes. a distributor which is separably connected to and rotates with the centrifuge rotor and which projects washing fluid into each of said tubes. and a metering peristaltic pump which transmits a predetermined quantity of washing fluid to the rotating distributor.
  • Blum US. Pat. No. 3.706.413 discloses automatic means for filling the centrifuge tubes with wash liquid.
  • a second piston pump was provided for producing air stream jets to purge residues of liquid from the nozzles of the distributor and project them into the respective centrifuge tubes.
  • indexing means were necessary to locate the centrifuge tubes precisely opposite the respective stationary distributor nozzles when the tube filling step took place.
  • the present invention obviates some of the drawbacks and disadvantages thereof by providing a distributor releaseably connected to the rotor of a swinging tube centrifuge. said distributor rotating with said rotor and projecting wash fluid into each of said tubes during rotation of the rotor. Furthermore. the distributor is supplied with metered amounts of wash fluid by means of a peristaltic pump. or the like. which transmits sterile or other biologically clean fluids without subjecting the latter to any possible contamination that would otherwise be incurred when passing such fluids through the interior of a pumping mechanism.
  • the peristaltic pump produces impulses externally upon a flexible tube made of vinyl or the like through which the fluid passes directly from the storage reservoir to the centrifuge distributor.
  • the cell washing process takes place automatically through the successive steps of tube filling. centrifugation and decantation from beginning to end without contamination or handling of wash fluids and without the necessity of performing repeated manual operations to flll and refill the swinging tubes of the centrifuge rotor.
  • FIG. 1 schematically represents the cell washing system of the apparatus herein, including a fragmentary portion of the reservoir. a front elevation of the peristaltic pump, and a side elevation of the contrifuge and decanting apparatus. part of the latter being broken away to show a portion of the interior thereof in elevation;
  • FIG. 2 is a vertical central section view of the upper portion of the centrifuge apparatus shown in FIG. I. some parts being shown in elevation and some parts in dotted outline;
  • FIG. 3 is an exploded partial view. in elevation. of a portion of the apparatus shown in FIG. 2, some parts being omitted and some parts being shown in section:
  • FIG. 4 is a top view taken on line 44 of FIG. 3., some parts being omitted and some parts being broken away;
  • FIG. 5 is a bottom view taken on line 5-5 of FIG. 3, some parts being broken away;
  • FIG. 6 is a greatly enlarged top view ofthe distributor shown in FIGS. 3 and 5, some parts being broken away and some parts being shown in dotted outline;
  • FIG. 7 is a section view taken on line 77 of FIG. 6:
  • FIG. 8 is a somewhat enlarged perspective view of a portion of the peristaltic pump shown in FIG. I.
  • FIG. 9 is a diagrammatic representation of the control circuit for the apparatus and system herein.
  • FIG. 1 a schematic representation of the improved cell washing system herein including an automatic cell washing apparatus.
  • an automatic cell washing apparatus generally designated 11
  • Base 12 contains an electric centrifuge motor 14 and electrical circuitry operated by a suitable control knob 15.
  • a suitable control knob 15 Supported on base 12 is a generally circular, bowl shaped centrifuge container 16 to which is connected. by means of hinge I7, a circular closure cover comprising an annular wall 18 and a cover plate 19 integrally formed therewith. Said cover is releaseably secured in position by a suitable lock element 20.
  • a centrifuge apparatus which will be described in greater detail hereinafter.
  • Mounted on the central top portion of cover 18 is an angled tubular fixture 21.
  • the system herein includes a wash water supply reservoir 22 connected by way of flexible tube 23 to fixture 21 on the centrifuge.
  • a peristaltic pump 24 operated by an electric motor 25.
  • An intermediate portion of tube 23 extends around a rotatable capstan 26 of peristaltic pump 24 (FIG. 8) whereby the spaced rollers 27 of said capstan produces successive peristaltic pinches on tube 23 in order to cause the wash fluid to flow therethrough to the centrifuge apparatus.
  • a suitable variable restrictor valve 28 may be incorporated into that portion of tube 23 extending between reservoir 22 and pump 24 for predetermining or controlling the quantity of fluid that passes from the reservoir to the centrifuge.
  • a drive shaft 32 connected to and rotated by electric motor 14 is base section 12. See FIGS. 2 and 4.
  • a circular centrifuge bowl 33 Mounted on shaft 32 and rotatable therewith is a circular centrifuge bowl 33. Secured to the central interior portion of said bowl. and rotating therewith. is a circular base element 34. Extending through and upwardly from base 34 and located axially relative to drive shaft 32 is a spindle 36 which is rotatably driven by shaft 32. Removably mounted over and rotated by spindle 36 is a tubular hub 37 to the upper end of which is axially connected horizontal carrier plate 38 extending outwardly from said hub. Arrayed in a circle coaxially with hub 37 near the outer portion of plate 38 is a plurality of evenly spaced apart slots 39, each of which is pivotally engaged by the curved hinge 41 of a skeleton tube carrier.
  • Each tube carrier 42 comprises an elongated spine having one or more spaced apart integrally formed circular tube bands or rings 43.
  • the bottom portion of tube carrier 42 terminates in an outwardly curved supported flange 44.
  • Each swinging tube carrier accommodates an elongated centrifuge tube 46.
  • tube carriers 42 and tubes 46 When the centrifuge is at rest or in the decanting position. tube carriers 42 and tubes 46 are positioned in a vertical or near vertical position. When the apparatus is in the centrifuging condition. tube carriers 42 and tubes 46 swing outwardly. as shown in FIG. 2, where they are retained at the angled position by virtue of the outward ends of flanges 44 bearing by centrifugal action against the inner wall of bowl 33. It is understood that the various suitable mechanisms for releaseably locking the tube carriers 42 in the decanting position. as disclosed in US. Pat. No. 3.420.437. or mechanisms similar thereto. will be utilized in the apparatus described herein.
  • Knob 48 Secured to the upper interior end of tubular hub 37 is a stub 47. the upper end of which terminates in a knob 48 extending upwardly above carrier plate 38. Knob 48 has upper and lower inwardly beveled annular surfaces 49 and 51, respectively. the function of which will be described hereinafter.
  • An annular collector trough 56 located within annular closure wall 18, has an integrally formed annular flange 57 which is secured to a peripheral portion of the bottom surface of cover plate 19. See FIG. 2.
  • the bottom of collector 56 has an integrally formed. inwardly extending annular shelf 58, the inner circular edge of which is located in a position where decanting liquids are received from centrifuge tubes 46 when the latter are rotated in the vertical or near vertical position.
  • annular shelf 58 In one location of shelf 58, at a juncture with the vertical portion of collector trough 56, there is a drain outlet 59 to which is connected one end of flexible drain tube 61 which extends through a suitable aperture in annular wall 18. The other end of tube 61 (not shown) is located in a suitable place for collecting or disposinng of the decanted materials.
  • central base portion 62 Secured to the inner central top portion of cover plate 19 is the central base portion 62 of a downwardly and outwardly extending annular baffle 63.
  • the vertical portion of tubular fixture 21 is secured to cover plate 19 by means of a circular shield 64 which closes the central apertures in cover plate 19 and in base portion 62, respectively.
  • the lower end of fixture 21 has an integrally formed port element nozzle 66 which extends a short distance below the inner surface of base portion 62 into the interior of the centrifuge apparatus.
  • the distributor, generally designated 70. comprises an assembly of two releaseably connected elements. namely, a circular nozzle holder 71 and a closure cover 72. secured to the nozzle holder by means of spaced screws 73. See FIGS. 2, 4, 5, 6 and 7.
  • the bottom portion of nozzle holder 71 has a central circular recess 74 bounded on two sides by elongated arcuate niches 75 spaced l apart.
  • Located in circular array on the peripheral bottom portion of cover 72 are integrally formed.
  • downwardly extending spaced legs 76 that define a plurality of alignment recesses. the function of which will be described hereinafter.
  • Secured fast by means of a screw 77 on the inner end of recess 74 is the base of a spring element 78 having integrally formed.
  • Nozzle holder 71 has a plurality of radially extending spaced channels 83 oriented downwardly and outwardly from the top center of said holder. Located in the outer portion of channels 83 are. respective tubular nozzles 84, the outer ends of which extend outwardly and in radial array toward corresponding centrifuge tubes 46. Access to the inner ends of channels 83 is provided by a central aperture in cover 72 into and through which wash fluid is transmitted from nozzle 66.
  • each nozzle 84 may be cut at an angle relative to the axis thereof so that fluid emerging therefrom may be prevented from spraying upwardly.
  • the wash fluid being delivered to the well formed by aperture 85 and the top apex dome 86 of holder 71 is caused by the rotation of holder 71 at centrifugal speed to expel said fluid in jet streams through nozzles 84 into the respective centrifuge tubes 46.
  • suitable equidistant spacing of channels 83 in relation to the symmetrically shaped conical dome 86 the distribution of substantially equal amounts of fluid into tubes 46 is ensured.
  • nozzles 84 are always properly aligned with respective tubes 46.
  • FIG. 9 represents on embodiment of typical control system for operating the automatic cell washing apparatus herein.
  • the centrifuge motor to which shaft 32 is connected is operated by its timer. controlled by knob 15.
  • the peristaltic pump motor is controlled by its separate timer operated by a second knob. not shown. Both control knobs are mounted on the sloping front panel of base section 12. After the biological specimens have been inserted into each of the centrifuge tubes 46. the centrifuge motor 14 is started to cause rotation of carrier plate 38 whereby tubes 46 swing outwardly to the angled centrifuging position as shown in FIG. 2.
  • the centrifuge timer is adjusted to determine the duration of centrifugation.
  • a signal in the electrical control system actuates a solenoid selector switch to cause operation of peristaltic pump 24 by pump motor 25 whereupon a predetermined quantity of fluid is transmitted from reservoir 22 through tube 23 into the well of distributor 70 whence the wash liquid is projected centrifugally through nozzles 84 into respective tubes 46 in a quantity predetermined by the timer connected to the pump motor and. additionally. by the adjustment of restrictor valve 28.
  • the setting on the pump motor timer may be determined and calibrated so that the operator can set the on time ofthe pump motor for a requisite period for filling the centrifuge tubes 46 with the proper amount of wash liquid.
  • the centrifuge timer automatically stops the rotation of the centrifuge to permit test tubes 46 to descend to their suspended position where they are all automatically secured in a vertical or near vertical decanting position as shown by the dotted outlines in FIG. 2, and retained there by means described. for example. in U.S. Pat. No. 3.420.437. or by any other suitable means. Thereafter. the timer again activates the centrifuge motor 14 to cause rotation of test tubes 46 in the vertical or near vertical position at a slower speed for a predetermined period whereby decantation of the supernatant fluid takes place from said tubes onto shelf 58 whence said fluid descends by gravity through drain outlet 59 and drain tube 61 to a suitable reservoir or sink.
  • the foregoing steps define a single cell-washing cycle.
  • the tube restraining mechanism (not shown) on the centrifuge automatically releases the holders 42 of tubes 46.
  • the centrifuge motor is started again in the centrifugation mode to cause said tubes to assume the angled centrifuging position as shown in FIG. 2.
  • the pump motor 25 becomes automatically activated to cause peristaltic pump 24 to draw fresh saline solution from reservoir 22 and transmit it through tube 23 and fixture 21 into the well formed by the aperture 85.
  • the centrifuge rotor rotates at approximately 1.200 rpm. while centrifugal force propels the saline solution through nozzles 84 into the respective centrifuge tubes 46.
  • nozzles 84 project slightly into the upper open ends of tubes 46 which are spinning and are maintained at an angle of approximately 42 from the vertical. in one embodiment of the apparatus. From the time the saline solution enters the distributor to the time it impinges upon the previously centrfigued residuum or button in each tube 46 and is accelerated by increasing centrifugal force. the velocity of the fluid being projected into the respective tubes 46 resuspends the residual materials therein and accomplishes the washing action that is intended by the apparatus.
  • pump motor 25 is stopped by its respective timer after which further centrifugation is continued by centrifuge motor 14 until the requisite predetermined time has elapsed in accordance with the setting of the centrifuge motor timer. Thereafter. the tube filling. cell washing centrifugation. and decanting cycles may be repeated as required by clinical or experimental considerations.
  • a suitable wash cycle selector may be included in the control circuit for the-apparatus herein in order to permit the operator thereof to select the number of complete tube filling. centrifuging. and decantation cycles to be performed.
  • the cell washing apparatus disclosed herein provides a virtually closed system from the saline reservoir 22 to the drain aperture 59 whereby possible contamination and erroneous results are minimized oreliminated.
  • the unitary tube 23 which transmits saline from reservoir 22 to the aperture well 85 in distributor 70, the integrity of that solution is preserved. contrary to the necessity in other types of apparatus to pass through the interior of pump cylinders and the like.
  • Tube 23 encircles rotating capstan 26 and the pumping action is impressed upon the exterior of tube 23 by an array of three or more spaced rollers 27 that peristaltically pinch said tube to cause the movement of fluid therein.
  • the requisite amount of solution to be transmitted to centrifuge tubes 46 is empirically determined before the apparatus performs actual operating experiments. and this is accomplished by adjusting the flow control or restrictor valve 28 to calibrate the delivery volume of peristaltic pump 24.
  • Flow control valve 28 is in line" in tube 23 and it may be adjusted to accommodate the operation of the apparatus to the type of material of which tube 23 is made and to its internal diameter which determines its flow capacity.
  • distributor nozzles 84 are rotating at high speed during the transmission of washing fluid into centrifuge tubes 46. centrifugal force will automatically purge said tubes of any residual wash liquid so that no auxiliary purge devices and equipment are required to clear said tubes of the wash liquid.
  • centrifuge tubes 46 when the centrifuge tubes 46 are in the angled centrifuging position as illustrated in solid lines in FIG. 2. the top portions of said tubes are located within respective recesses between legs 76 whereby accidental skewing of said tubes on their respcctive hinges 41 is prevented. thus ensuring that liquids from nozzles 84 will be directed into the respective centrifuge tubes.
  • a resilient 0-ring 87 therebetween. made of a suitable material such as nylon. Teflon. rubber. or the like.
  • a system for treating biological materials and the like comprising: a swinging tube centrifuge having a rotor. a plurality of swinging tube holders mounted on said rotor for holding centrifuge tubes. and a variable speed drive for said rotor; a distributor mounted on and rotating with said centrifuge. said distributor having a plurality of spaced. radially extending nozzles equal in number to said tube holders. said nozzles being aligned with but unconnected to said tube holders.
  • said tube holders being pivotally mounted on said rotor so that the mouth ofthe centrifuge tubes held by said swinging tube holders is brought into close proximity with said nozzles when said rotor is in high speed rotation; a source of treatment fluid; tube means connected between said source and said distributor for transmitting said fluid to said distributor; and pulsing pump means operating upon an cxterior portion of said tube means for urging said fluid from said source to said distributor.
  • valve control means between said source and said pump for adjusting and controlling the flow of said fluid between said source and said distributor.
  • said drive is a two-speed drive for said centrifuge.
  • said tubes assuming an outwardlyextended array in the high speed mode of said drive.
  • said tubes assuming a vertical decanting position in the low speed mode ofsaid drive and said-nozzles projecting fluid into respective tubes only when the latter are in the centrifuging mode.
  • the system for treating biological materials and the like comprising a swinging tube centrifuge. a multispeed motor for rotating said centrifuge at a predetermined centrifuging speed. and alternately at a lower decanting speed. a source of treatment fluid. means for transmitting said fluid to said centrifuge. pulsing pump means operating on said transmitting means for urging said fluid from said source to said centrifuge. a pump motor for said pump. electrical circuit means connected between said centrifuge motor and said pump motor for causing said centrifuge motor to operate alternately for the respective centrifuging and decanting modes of said centrifuge. and for causing said pump motor to operate to transmit treatment fluid to said centrifuge driving the centrifuging mode.
  • control means in said electrical circuit for predetermining the timing and sequence of each of the tube filling. centrifuging and decanting modes of the system.
  • the system for treating biological materials and the like comprising a swinging tube centrifuge. a plurality of swinging tubes in circular array on said centrifuge. a source of treatment fluid. a distributor removably mounted on and rotating with said centrifuge for projecting treatment fluid into respective centrifuge tubes. said distributor comprising a plurality of spaced radially extending nozzles equal in number .to said swinging tubes. said nozzles being aligned with but unconnected to said swinging tubes. means for alternately rotating said centrifuge at a centrifuging speed and at a lower decanting speed. means for transmitting a metered quantity of treatmentfluid into and through said distributor and into said centrifuge tubes during the centrifuge mode of the centrifuge. said centriguge tubes being pivotally mounted to swing into close proximity with said nozzles during the centrifuging mode.
  • timing means connected between said rotating means and said fluid transmitting means to control and selectively predetermine the operational times of the separate and successive tube filling. centrifuging. and decanting functions of said system.
  • said fluid transmitting means comprises a peristaltic pump connected between said sourceand said centrifuge.
  • said fluid transmitting means comprises a flexible tube connected between said source and said distributor and a peristaltic pump operative upon the exterior portion of said tube to cause the movement of said fluid from said source to said distributor.

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
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US364150A 1973-05-25 1973-05-25 Cell washing centrifuge apparatus and system Expired - Lifetime US3877634A (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US364150A US3877634A (en) 1973-05-25 1973-05-25 Cell washing centrifuge apparatus and system
GB2315674A GB1470806A (en) 1973-05-25 1974-05-23 Treatment centrifuges
JP5867674A JPS5710776B2 (enrdf_load_stackoverflow) 1973-05-25 1974-05-24
CA200,830A CA1007213A (en) 1973-05-25 1974-05-24 Cell washing centrifuge apparatus and system
DE19742425165 DE2425165A1 (de) 1973-05-25 1974-05-24 Vorrichtung zur zentrifugalen behandlung bzw. zum waschen von biologischen proben

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US364150A US3877634A (en) 1973-05-25 1973-05-25 Cell washing centrifuge apparatus and system

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US3877634A true US3877634A (en) 1975-04-15

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US364150A Expired - Lifetime US3877634A (en) 1973-05-25 1973-05-25 Cell washing centrifuge apparatus and system

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US (1) US3877634A (enrdf_load_stackoverflow)
JP (1) JPS5710776B2 (enrdf_load_stackoverflow)
CA (1) CA1007213A (enrdf_load_stackoverflow)
DE (1) DE2425165A1 (enrdf_load_stackoverflow)
GB (1) GB1470806A (enrdf_load_stackoverflow)

Cited By (37)

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US3981438A (en) * 1975-06-19 1976-09-21 E. I. Du Pont De Nemours And Company Centrifuge rotor having swinging members with variable pivot points
US4069968A (en) * 1976-11-01 1978-01-24 Union Carbide Corporation Disposable tubing harness for use with blood washing apparatus
US4190530A (en) * 1978-04-03 1980-02-26 E. I. Du Pont De Nemours And Company Centrifugal method and apparatus for processing fluid materials
WO1981001255A1 (en) * 1979-11-01 1981-05-14 American Hospital Supply Corp Decanting centrifuge
US4431423A (en) * 1982-03-10 1984-02-14 E. I. Du Pont De Nemours & Co. Cell washing apparatus having radially inwardly directed retaining arms
US4589864A (en) * 1984-11-05 1986-05-20 E. I. Du Pont De Nemours And Company Centrifuge rotor having a resilient trunnion
US4817453A (en) * 1985-12-06 1989-04-04 E. I. Dupont De Nemours And Company Fiber reinforced centrifuge rotor
US5178602A (en) * 1990-02-07 1993-01-12 Wells John R Automatic decanting centrifuge
US5199937A (en) * 1989-08-24 1993-04-06 Kurashiki Boseki Kabushiki Kaisha Centrifugal separator
US5456652A (en) * 1993-02-24 1995-10-10 Firma Andreas Hettich Rotor for a swiveling beaker centrifuge
US5707331A (en) * 1995-05-05 1998-01-13 John R. Wells Automatic multiple-decanting centrifuge
US5767381A (en) * 1996-01-31 1998-06-16 Hitachi, Ltd. Centrifuge model test apparatus
US6056684A (en) * 1994-06-15 2000-05-02 Massachusetts Institute Of Technology Locking centrifuge rotor cover assembly
WO2000047328A1 (en) * 1999-02-11 2000-08-17 Seward Limited Centrifuge rotors
US6197579B1 (en) 1997-02-14 2001-03-06 Dendreon Corporation Cell washing device and method
US6235537B1 (en) * 1996-06-20 2001-05-22 Cytek Corporation Methods for washing cells
US6398972B1 (en) 1999-04-12 2002-06-04 Harvest Technologies Corporation Method for producing platelet rich plasma and/or platelet concentrate
US6623959B2 (en) 2001-06-13 2003-09-23 Ethicon, Inc. Devices and methods for cell harvesting
US20030211928A1 (en) * 2001-09-24 2003-11-13 Dolecek Victor D. Method of separating and collecting components from a fluid
US20030216237A1 (en) * 2002-05-17 2003-11-20 Hitachi Koki Co., Ltd. Bio cell cleaning centrifuge having detachable chamber body
US20030216238A1 (en) * 2002-05-17 2003-11-20 Hitachi Koki Co., Ltd. Bio cell cleaning centrifuge having bio cell cleaning rotor provided with cleaning liquid distributor
USRE38730E1 (en) * 1995-05-05 2005-04-26 Harvest Technologies Corporation Automatic multiple-decanting centrifuge and method of treating physiological fluids
US20050277538A1 (en) * 2004-06-14 2005-12-15 Michael Sherman Automated device for homogenization and resuspension of substances, disintegration of cells, disruption of tissues and centrifugation of these media
US20060094865A1 (en) * 2004-10-29 2006-05-04 Kapur Terri A Intraoperative method for isolating and concentrating autologous growth factors and for forming residual autologous growth factor compositions
US7347948B2 (en) 2001-04-09 2008-03-25 Ateriocyte Medical Systems, Inc. Blood centrifuge having clamshell blood reservoir holder with index line
US20080207211A1 (en) * 2004-09-15 2008-08-28 Samsung Electronics Co., Ltd. Method and apparatus for indicating preferred layer information in multimedia broadcast/multicast service (MBMS)
US20080318755A1 (en) * 2007-06-21 2008-12-25 Hitachi Koki Co., Ltd. Bio cell cleaning centrifuge and bio cell cleaning rotor used in the same
US20110033924A1 (en) * 2008-01-09 2011-02-10 Cytosystems Limited Apparatus and Method for Filtering Biological Material
US20110160031A1 (en) * 2002-08-02 2011-06-30 Harvest Technologies Corporation Decanting centrifuge with vibration isolation
US20150003183A1 (en) * 2013-07-01 2015-01-01 Chopin Technologies Shaking device
US20150005150A1 (en) * 2013-07-01 2015-01-01 Chopin Technologies Shaking and centrifuging device
WO2019106185A1 (en) * 2017-12-01 2019-06-06 Christophe Blanc Peristaltic pump cassette
US10654000B2 (en) 2016-07-13 2020-05-19 Fenwal, Inc. Cell processing system and method with centralized data management, monitoring and/or control
US10772921B1 (en) * 2019-05-28 2020-09-15 Shaanxi University Of Science And Technology Device for high pressure spray and counter-current precipitation extraction of herbal medicine and a method for extracting herbal medicine using the same
US11062805B2 (en) 2016-03-14 2021-07-13 Fenwal, Inc. Cell processing system and method with process parameter control
US11191879B2 (en) 2016-05-27 2021-12-07 Fenwal, Inc. Cell processing system and method with preliminary process evaluation
US20220184639A1 (en) * 2019-06-27 2022-06-16 Eppendorf Himac Technologies Co., Ltd. Centrifuge

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JPS54167860U (enrdf_load_stackoverflow) * 1978-05-17 1979-11-27
DE4124249A1 (de) * 1991-07-22 1993-01-28 Peter Macht Verfahren zur aufarbeitung und wiederverwendung gebrauchter heizkostenverteilermessampullen
DE4323844A1 (de) * 1993-07-16 1995-01-19 Hettich Andreas Fa Waschzentrifuge
DE4324893C2 (de) * 1993-07-24 1997-08-28 Hettich Andreas Fa Röhrchen-Durchlaufzentrifuge
DE19948201B4 (de) * 1999-10-06 2010-12-09 Thermo Electron Led Gmbh Zentrifugen-Rotor

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CA1007213A (en) 1977-03-22
DE2425165C2 (enrdf_load_stackoverflow) 1991-01-31
JPS5710776B2 (enrdf_load_stackoverflow) 1982-02-27
DE2425165A1 (de) 1974-12-12
GB1470806A (en) 1977-04-21
JPS5022693A (enrdf_load_stackoverflow) 1975-03-11

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