US3870146A - Wright{3 s stain packet - Google Patents
Wright{3 s stain packet Download PDFInfo
- Publication number
- US3870146A US3870146A US423456A US42345673A US3870146A US 3870146 A US3870146 A US 3870146A US 423456 A US423456 A US 423456A US 42345673 A US42345673 A US 42345673A US 3870146 A US3870146 A US 3870146A
- Authority
- US
- United States
- Prior art keywords
- stain
- packet
- solution
- wright
- slide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000007788 liquid Substances 0.000 claims abstract description 8
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims abstract description 6
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 6
- 239000011734 sodium Substances 0.000 claims abstract description 6
- NALREUIWICQLPS-UHFFFAOYSA-N 7-imino-n,n-dimethylphenothiazin-3-amine;hydrochloride Chemical compound [Cl-].C1=C(N)C=C2SC3=CC(=[N+](C)C)C=CC3=NC2=C1 NALREUIWICQLPS-UHFFFAOYSA-N 0.000 abstract description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 27
- 239000000243 solution Substances 0.000 description 20
- 239000000203 mixture Substances 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 238000009472 formulation Methods 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 238000010186 staining Methods 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 4
- 239000008399 tap water Substances 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000012062 aqueous buffer Substances 0.000 description 1
- 239000008366 buffered solution Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000002657 fibrous material Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000005470 impregnation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229960000907 methylthioninium chloride Drugs 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000011120 smear test Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000012192 staining solution Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D75/00—Packages comprising articles or materials partially or wholly enclosed in strips, sheets, blanks, tubes or webs of flexible sheet material, e.g. in folded wrappers
- B65D75/28—Articles or materials wholly enclosed in composite wrappers, i.e. wrappers formed by associating or interconnecting two or more sheets or blanks
- B65D75/30—Articles or materials enclosed between two opposed sheets or blanks having their margins united, e.g. by pressure-sensitive adhesive, crimping, heat-sealing, or welding
Definitions
- the solution is buffered with sodium barbitol.
- the solution may include Giemsa Stain.
- the present invention relates to a single use Wrights Stain packet.
- Wright Stain is a well known mixture of eosin with methylene blue, available commercially in powder form.
- the powder is dissolved in methanol, normally between 0.1 and 0.3 grams per 100ml methanol.
- Some formulations recommend inclusion of glycerine to enhance solubility of the dye.
- a typical practice is to age a freshly prepared solution at 37C., then filter and store in dark bottles use.
- a suggested technique for Wrights Stain is to add the methanol stain solution to the blood smear slide, let it stand for about 1 to 3 minutes, then replace the methanol stain solution with the aqueous buffer solution, let the buffered solution stay on the slide for 3 to 5 minutes, whereafter the slide may be rinsed in tap or distilled water and dried.
- a principal object of the present invention is to provide a Wrights Stain packet of a single use nature adapted to the needs of the laboratory technician.
- the Wrights Stain packet of the present, invention comprises a single use, air and liquid impermeable package which contains a slide sized pad impregnated with a buffered methanolic solution of Wrights Stain.
- the pad When opened, the pad may be removed from the wrapper by tweezers and placed over the blood smear on a slide, whereupon it releases the stain solution in a controlled and uniform fashion. After about 30 seconds, the slide has become stained. The slide, with the pad still thereon, is rinsed in tap water. If the slide is held under a gentle stream of tap water, the pad floats away. I
- FIGS. 1, 2, 3, and 4 are diagrammatic views illustrating the structure and use of the Wrights stain packet.
- the packet 10 consists of a stain impregnated pad 14 sealed between upper and lower air and liquid impermeable wrapper sections 11 and 12, the sections being adhered together at 13 so as to enclose pad 14.
- pad 14 When wrapper sections 11, 12 are peeled apart, pad 14 may be removed by tweezer l5 and placed upon a slide 16. Subsequently the pad may be lifted off slide 16 by disposing slide 16 under the water stream 18 available from any convenient water tap 17.
- a Wrights Stain single use packet offers many advantages.
- use of the stain is easier than staining by existing techniques, permitting less skilled personnel to Wrights Stain a slide.
- the pad applied staining solution eliminates the real, or at least the potential, mess of a bulk liquid staining technique.
- Working time for staining a slide is reduced.
- the need for a special buffer solution is eliminated altogether; existing water supplies may be employed for rinsing.
- the prepackaged Wrights Stain packet allows for a high degree of stain uniformity and reproduceability. Variations in staining characteristics attributable to differences solution to solution can be eliminated, through mass produced uniformity packet to packet.
- the preferred formulation of the Wrights Stain solution herein described is relatively stable, thereby creating a high shelf life packet.
- the packet wrapping itself forms no part of the present invention.
- Standard aluminum foil packing material commonly employed to package many products such as for example, single servings ofinstant coffee
- the wrapping material for the packet must be both air impermeable and water impermeable.
- a conventional woven or non-woven thin fabric may be employed for purposes of the Wrights Stain impregnated pad. It is only necessary that the fibrous material be inert, nonreactive and capable of acting as a carrier with uniform impregnation. Its purpose is to cover the blood smeared slide, to spread the Wright Stain uniformly on the face of the slide. Thus, the pad should be about the size ofthe standard microscope slide employed for blood smear tests using Wrights Stain.
- a barbitol buffered Wrights Stain is particularly desirable for the single use packet of the present invention. It has also been found that inclusion of Giemsa Stain within the formulation produces superior slide results. Accordingly, a preferred mode of the invention is barbitol buffered Wrights Stain and Giemsa Stain in methanol formulation.
- such a formulation is prepared as follows:
- Powdered certified Wrights Stain (SKG) is added slowly to hot methanol (350 liters) with constant stirring. The mixing is continued for 8 hours. Thereafter, the methanol solution is permitted to cool to room temperature and filtered (through Whatman No. 1 paper).
- the powdered Giemsa stain (0.921(6) is similarly dissolved in hot methanol liters), stirred, cooled and filtered as above.
- the dye solutions are kept separate in sealed containers and aged for 2 weeks. During this period, occasional agitation is effected. At the end of the aging period, solution volumes are adjusted (by addition, appropriately, of methanol to 350 liters and 125 liters respectively), to make up any evaporation losses.
- a buffer solution is prepared as follows:
- the actual buffer solution is prepared by mixing together 10.5 liters of the sodium barbitol containing solution, 10.5 liters of the 0.1 NHcl and the 4.0 liters of sodium chloride.
- the final pH of this solution should be pH 7.3 i 0.1. If adjustment is necessary to achieve this What is claimed:
- a Wrights Stain packet which comprises a single use air and liquid impermeable package containing therein a microscope slide sized pad impregnated with a buffered methanolic solution of Wrights Stain.
Landscapes
- Chemical & Material Sciences (AREA)
- Composite Materials (AREA)
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
A Wright''s Stain packet which comprises a single use air and liquid impermeable package and containing therein a slide sized pad impregnated with a buffered methanolic solution of Wright''s Stain. Preferably the solution is buffered with sodium barbitol. The solution may include Giemsa Stain.
Description
United States Patent 1 Greenfield et al.
[ 1 Mar. 11, 1975 WRIGHTS STAIN PACKET Inventors: Walter Greenfield, Ardlsey;
Raymond Berg, Scarsdale, both of NY. I
Assignee: Sci-Med Laboratories, Inc., Hartsdale, N.Y.
Filed: Dec. 10, 1973 Appl. No 423,456
US. Cl. 206/84 Int. Cl. B65d 81/00 Field of Search 206/84, 205, 361, 484;
l5/lO4.93
References Cited UNITED STATES PATENTS 9/1960 Maso et al. 206/484 3,057,467 10/1962 Williams 206/361 3,132,744 5/ l 964 Scharf 3,495,926 2/1970 Naz 206/84 X Primary Examiner-Leonard Summer Attorney, Agent, or FirmFidelman, Wolffe & Leitner {57] ABSTRACT A Wrights Stain packet which comprises a single use air and liquid impermeable package and containing therein a slide sized pad. impregnated with a buffered methanolic solution of Wrights Stain.
Preferably the solution is buffered with sodium barbitol. I The solution may include Giemsa Stain.
3 Claims, 4 Drawing Figures WRIGHTS STAIN PACKET The present invention relates to a single use Wrights Stain packet.
Wright Stain is a well known mixture of eosin with methylene blue, available commercially in powder form. For use, the powder is dissolved in methanol, normally between 0.1 and 0.3 grams per 100ml methanol. Some formulations recommend inclusion of glycerine to enhance solubility of the dye. A typical practice is to age a freshly prepared solution at 37C., then filter and store in dark bottles use. A separate buffer solution pH 6.4 7.4, usually with a phosphate buffer, is employed as part of the slide staining procedure.
A suggested technique for Wrights Stain is to add the methanol stain solution to the blood smear slide, let it stand for about 1 to 3 minutes, then replace the methanol stain solution with the aqueous buffer solution, let the buffered solution stay on the slide for 3 to 5 minutes, whereafter the slide may be rinsed in tap or distilled water and dried.
- Normally, such a procedure is carried out manually, requiring a certain degree of skill on the part of the laboratory technician. Automated systems which have been suggested require expensive machinery, special stain packaging and are relatively time consuming. A dry strip which has been proposed offers some advantages but requires immersion to activate the stain ingredients thereron.
A single use Wrights Stain packet would be of considerable value to the laboratory technician.
A principal object of the present invention is to provide a Wrights Stain packet of a single use nature adapted to the needs of the laboratory technician.
Briefly stated, the Wrights Stain packet of the present, invention comprises a single use, air and liquid impermeable package which contains a slide sized pad impregnated with a buffered methanolic solution of Wrights Stain. When opened, the pad may be removed from the wrapper by tweezers and placed over the blood smear on a slide, whereupon it releases the stain solution in a controlled and uniform fashion. After about 30 seconds, the slide has become stained. The slide, with the pad still thereon, is rinsed in tap water. If the slide is held under a gentle stream of tap water, the pad floats away. I
For further understanding of the inventors reference is made to the attached self-explanatory drawing wherein:
FIGS. 1, 2, 3, and 4 are diagrammatic views illustrating the structure and use of the Wrights stain packet.
As is apparent in the drawing, the packet 10 consists of a stain impregnated pad 14 sealed between upper and lower air and liquid impermeable wrapper sections 11 and 12, the sections being adhered together at 13 so as to enclose pad 14.
When wrapper sections 11, 12 are peeled apart, pad 14 may be removed by tweezer l5 and placed upon a slide 16. Subsequently the pad may be lifted off slide 16 by disposing slide 16 under the water stream 18 available from any convenient water tap 17.
A Wrights Stain single use packet, according to the present invention, offers many advantages. Thus, use of the stain is easier than staining by existing techniques, permitting less skilled personnel to Wrights Stain a slide. The pad applied staining solution eliminates the real, or at least the potential, mess of a bulk liquid staining technique. Working time for staining a slide is reduced. The need for a special buffer solution is eliminated altogether; existing water supplies may be employed for rinsing. The prepackaged Wrights Stain packet allows for a high degree of stain uniformity and reproduceability. Variations in staining characteristics attributable to differences solution to solution can be eliminated, through mass produced uniformity packet to packet. Moreover, the preferred formulation of the Wrights Stain solution herein described is relatively stable, thereby creating a high shelf life packet.
The packet wrapping itself forms no part of the present invention. Standard aluminum foil packing material commonly employed to package many products (such as for example, single servings ofinstant coffee) may be used to form a peelable, air-tight, liquid-tight wrapping for Wrights Stain impregnated pad. To repeat, the wrapping material for the packet must be both air impermeable and water impermeable.
In like fashion, the pad itself forms no part of the present invention, A conventional woven or non-woven thin fabric may be employed for purposes of the Wrights Stain impregnated pad. It is only necessary that the fibrous material be inert, nonreactive and capable of acting as a carrier with uniform impregnation. Its purpose is to cover the blood smeared slide, to spread the Wright Stain uniformly on the face of the slide. Thus, the pad should be about the size ofthe standard microscope slide employed for blood smear tests using Wrights Stain.
Although typical known Wrights Stain formulations may be employed for practice of the present invention, 7
it has been found that a barbitol buffered Wrights Stain is particularly desirable for the single use packet of the present invention. It has also been found that inclusion of Giemsa Stain within the formulation produces superior slide results. Accordingly, a preferred mode of the invention is barbitol buffered Wrights Stain and Giemsa Stain in methanol formulation.
According to a preferred mode of the invention, such a formulation is prepared as follows:
Powdered certified Wrights Stain (SKG) is added slowly to hot methanol (350 liters) with constant stirring. The mixing is continued for 8 hours. Thereafter, the methanol solution is permitted to cool to room temperature and filtered (through Whatman No. 1 paper).
Separately, the powdered Giemsa stain (0.921(6) is similarly dissolved in hot methanol liters), stirred, cooled and filtered as above.
The dye solutions are kept separate in sealed containers and aged for 2 weeks. During this period, occasional agitation is effected. At the end of the aging period, solution volumes are adjusted (by addition, appropriately, of methanol to 350 liters and 125 liters respectively), to make up any evaporation losses.
A buffer solution is prepared as follows:
I. Dissolve 0.340 kilograms of sodium chloride in 4.0 liters of water;
II. Prepare 0.1 normal hydrochloric acid (12.1);
Ill. Dissolve 0.353 kilograms of sodium barbitol and 0.233 kilograms of sodium acetate in l2.0"liters of water.
The actual buffer solution is prepared by mixing together 10.5 liters of the sodium barbitol containing solution, 10.5 liters of the 0.1 NHcl and the 4.0 liters of sodium chloride. The final pH of this solution should be pH 7.3 i 0.1. If adjustment is necessary to achieve this What is claimed:
1. A Wrights Stain packet which comprises a single use air and liquid impermeable package containing therein a microscope slide sized pad impregnated with a buffered methanolic solution of Wrights Stain.
2. The packet of claim 1 wherein the solution is buffered with sodium barbitol.
3. The packet of claim 1 wherein the solution includes therein Giemsa stain.
Claims (3)
1. A WRIGHT''S STAIN PACKET WHICH COMPRISES A SINGLE USE AIR AND LIQUID IMPERMEABLE PACKAGE CONTAINING THEREIN A MICROSCOPE SLIDE SIZED PAD IMPREGMNATED WITH A BUFFERED METHANOLIC SOLUTION OF WRIGHT''S STAIN.
1. A Wright''s Stain packet which comprises a single use air and liquid impermeable package containing therein a microscope slide sized pad impregnated with a buffered methanolic solution of Wright''s Stain.
2. The packet of claim 1 wherein the solution is buffered with sodium barbitol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US423456A US3870146A (en) | 1973-12-10 | 1973-12-10 | Wright{3 s stain packet |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US423456A US3870146A (en) | 1973-12-10 | 1973-12-10 | Wright{3 s stain packet |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| US3870146A true US3870146A (en) | 1975-03-11 |
Family
ID=23678972
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US423456A Expired - Lifetime US3870146A (en) | 1973-12-10 | 1973-12-10 | Wright{3 s stain packet |
Country Status (1)
| Country | Link |
|---|---|
| US (1) | US3870146A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030039781A1 (en) * | 1998-10-29 | 2003-02-27 | Closure Medical Corporation | Polymeric containers for 1,1-disubstituted monomer compositions |
| US20040043495A1 (en) * | 2002-08-29 | 2004-03-04 | Stokes Barry O. | Method and staining reagent for staining hematology sample in an automated staining apparatus |
| CN104390832A (en) * | 2014-11-07 | 2015-03-04 | 刘志军 | Formula of rapid Wright's stain suitable for blood cells, and preparation method of rapid Wright's stain |
| WO2017146506A1 (en) * | 2016-02-23 | 2017-08-31 | 노을 주식회사 | Blood staining patch, and method and apparatus for testing blood using same |
| WO2017146505A1 (en) * | 2016-02-23 | 2017-08-31 | 노을 주식회사 | Substance-marking patch, and method and apparatus for tissue diagnosis using same |
| CN107300495A (en) * | 2017-06-15 | 2017-10-27 | 托克逊县夏乡畜牧业服务中心 | A kind of colouring method of pyriform worm blood film compound stain solution and blood film |
| US10345204B2 (en) | 2016-02-23 | 2019-07-09 | Noul Co., Ltd. | Contact-type patch, staining method using the same, and manufacturing method thereof |
| EP3428644A4 (en) * | 2016-02-23 | 2019-10-23 | Noul Co., Ltd. | BLOOD COLORING PATCH, AND METHOD AND APPARATUS FOR BLOOD ANALYZING USING THE SAME |
| KR20210013275A (en) * | 2016-02-23 | 2021-02-03 | 노을 주식회사 | Labeling substance storing patch, tissue diagnostic method using the patch and device using the same |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2954116A (en) * | 1957-05-06 | 1960-09-27 | Johnson & Johnson | Rupturable seal package with sterilized moist contents |
| US3057467A (en) * | 1959-11-18 | 1962-10-09 | Colgate Palmolive Co | Package for treating agents and disposable applicator forming a part thereof |
| US3132744A (en) * | 1961-10-02 | 1964-05-12 | Ben C Scharf | Disposable slide fixation fluid package |
| US3495926A (en) * | 1966-11-02 | 1970-02-17 | John F Naz | Method and apparatus for staining microslides |
-
1973
- 1973-12-10 US US423456A patent/US3870146A/en not_active Expired - Lifetime
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2954116A (en) * | 1957-05-06 | 1960-09-27 | Johnson & Johnson | Rupturable seal package with sterilized moist contents |
| US3057467A (en) * | 1959-11-18 | 1962-10-09 | Colgate Palmolive Co | Package for treating agents and disposable applicator forming a part thereof |
| US3132744A (en) * | 1961-10-02 | 1964-05-12 | Ben C Scharf | Disposable slide fixation fluid package |
| US3495926A (en) * | 1966-11-02 | 1970-02-17 | John F Naz | Method and apparatus for staining microslides |
Cited By (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030039781A1 (en) * | 1998-10-29 | 2003-02-27 | Closure Medical Corporation | Polymeric containers for 1,1-disubstituted monomer compositions |
| US20040043495A1 (en) * | 2002-08-29 | 2004-03-04 | Stokes Barry O. | Method and staining reagent for staining hematology sample in an automated staining apparatus |
| US6858432B2 (en) * | 2002-08-29 | 2005-02-22 | Wescor, Inc. | Method and staining reagent for staining hematology sample in an automated staining apparatus |
| US20050148083A1 (en) * | 2002-08-29 | 2005-07-07 | Stokes Barry O. | Staining reagent for staining hematology samples in an automated staining apparatus |
| US7303920B2 (en) | 2002-08-29 | 2007-12-04 | Wescor, Inc. | Staining reagent for staining hematology samples in an automated staining apparatus |
| CN104390832A (en) * | 2014-11-07 | 2015-03-04 | 刘志军 | Formula of rapid Wright's stain suitable for blood cells, and preparation method of rapid Wright's stain |
| AU2017224479B2 (en) * | 2016-02-23 | 2020-05-21 | Noul Co., Ltd. | Blood staining patch, method and device for blood test using the same |
| US11366043B2 (en) | 2016-02-23 | 2022-06-21 | Noul Co., Ltd. | Contact-type patch, staining method using the same, and manufacturing method thereof |
| US12216033B2 (en) | 2016-02-23 | 2025-02-04 | Noul Co, Ltd. | Contact-type patch, staining method using the same, and manufacturing method thereof |
| US10345204B2 (en) | 2016-02-23 | 2019-07-09 | Noul Co., Ltd. | Contact-type patch, staining method using the same, and manufacturing method thereof |
| US10371610B2 (en) | 2016-02-23 | 2019-08-06 | Noul Co., Ltd. | Contact-type patch, staining method using the same, and manufacturing method thereof |
| EP3428644A4 (en) * | 2016-02-23 | 2019-10-23 | Noul Co., Ltd. | BLOOD COLORING PATCH, AND METHOD AND APPARATUS FOR BLOOD ANALYZING USING THE SAME |
| WO2017146506A1 (en) * | 2016-02-23 | 2017-08-31 | 노을 주식회사 | Blood staining patch, and method and apparatus for testing blood using same |
| KR20210013275A (en) * | 2016-02-23 | 2021-02-03 | 노을 주식회사 | Labeling substance storing patch, tissue diagnostic method using the patch and device using the same |
| US11041842B2 (en) | 2016-02-23 | 2021-06-22 | Noul Co., Ltd. | Culturing patch, culturing method, culture test method, culture test device, drug test method, and drug test device |
| US11208685B2 (en) | 2016-02-23 | 2021-12-28 | Noul Co., Ltd. | Diagnostic method and device performing the same |
| US11360005B2 (en) | 2016-02-23 | 2022-06-14 | Noul Co., Ltd. | Contact-type patch, staining method using the same, and manufacturing method thereof |
| WO2017146505A1 (en) * | 2016-02-23 | 2017-08-31 | 노을 주식회사 | Substance-marking patch, and method and apparatus for tissue diagnosis using same |
| US11385144B2 (en) | 2016-02-23 | 2022-07-12 | Noul Co., Ltd. | Antibody-providing kit, antibody-containing patch, method and device for immunoassay using the same |
| KR20230002171A (en) * | 2016-02-23 | 2023-01-05 | 노을 주식회사 | Labeling substance storing patch, tissue diagnostic method using the patch and device using the same |
| US11740162B2 (en) | 2016-02-23 | 2023-08-29 | Noul Co., Ltd. | Contact-type patch, staining method using the same, and manufacturing method thereof |
| US11808677B2 (en) | 2016-02-23 | 2023-11-07 | Noul Co., Ltd. | Polymerase chain reaction patch, method and device for diagnosis using the same |
| US11898947B2 (en) | 2016-02-23 | 2024-02-13 | Noul Co., Ltd. | Diagnostic method and device performing the same |
| EP4293338A3 (en) * | 2016-02-23 | 2024-03-20 | Noul Co., Ltd. | Blood staining patch, and method and apparatus for testing blood using same |
| EP3982120B1 (en) * | 2016-02-23 | 2024-07-24 | Noul Co., Ltd. | Diagnostic method, and device for executing same |
| US12181390B2 (en) | 2016-02-23 | 2024-12-31 | Noul Co., Ltd. | Substance labeling patch, method and apparatus for tissue diagnosis using the same |
| CN107300495A (en) * | 2017-06-15 | 2017-10-27 | 托克逊县夏乡畜牧业服务中心 | A kind of colouring method of pyriform worm blood film compound stain solution and blood film |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US3870146A (en) | Wright{3 s stain packet | |
| ATE85781T1 (en) | PACKAGING DEVICE FOR A READY-TO-USE LIQUID SUBSTANCE MADE FROM A CONCENTRATED SUBSTANCE AND METHOD OF USE. | |
| US5260048A (en) | Tissue fixative solution and method | |
| US4839194A (en) | Methods of preparing tissue samples | |
| US5279313A (en) | Two component composition and method for bleaching hair | |
| CA2472039C (en) | Hydrogen peroxide and peracetic acid indicators and methods using the same | |
| DE3062373D1 (en) | Test composition for peroxidatively active substances, test device containing the composition, process for preparing same and method for the determination of peroxidatively active substances | |
| GB2196428B (en) | Apparatus for dyeing specimens automatically preparatory to microscopic examination | |
| DE3160110D1 (en) | Interference-resistant composition, device, method of preparing it and method for determining a peroxidatively active substance in a test sample | |
| IN172269B (en) | ||
| US3122420A (en) | Diagnostic formulation for ph determination | |
| GB2190104A (en) | Dyeing human hair with brazilin or haematoxylin in conjuntion with a cupric salt | |
| WO1998050012A1 (en) | Stable vitamin c concentrates | |
| DE3062644D1 (en) | Test composition for peroxidatively active substances, test device containing the composition, process for preparing same and method for the determination of peroxidatively active substances | |
| GB1381444A (en) | Toothpaste | |
| GB1396219A (en) | Method and reagent for testing blood | |
| GB1483574A (en) | Two-part composite device for histologic tissue processing and embedding | |
| US5389302A (en) | Chemiluminescent dye penetrant process and composition | |
| JP2545085B2 (en) | Specimen unit and sample preparation method using the specimen unit | |
| EP0068530A1 (en) | Package for foodstuffs, such as shell fish, which while in the packaged state will exude liquid, and a packaging method | |
| US3773464A (en) | Acid and direct dyes and fluorescent brightener concentrates with thiocyanates and acetylinic alcohol | |
| Jørgensen et al. | Mercury leakage of amalgam capsules | |
| RU2038576C1 (en) | Method of determining density of solid bodies | |
| FORSTEN et al. | Fluoride release from varnish‐coated silicates and from cavity liners and fissure sealants | |
| JPS5759627A (en) | Solubilization |