WO2017146506A1 - Blood staining patch, and method and apparatus for testing blood using same - Google Patents

Blood staining patch, and method and apparatus for testing blood using same Download PDF

Info

Publication number
WO2017146506A1
WO2017146506A1 PCT/KR2017/002030 KR2017002030W WO2017146506A1 WO 2017146506 A1 WO2017146506 A1 WO 2017146506A1 KR 2017002030 W KR2017002030 W KR 2017002030W WO 2017146506 A1 WO2017146506 A1 WO 2017146506A1
Authority
WO
WIPO (PCT)
Prior art keywords
patch
pa
blood
material
sample
Prior art date
Application number
PCT/KR2017/002030
Other languages
French (fr)
Korean (ko)
Inventor
이동영
임찬양
김경환
신영민
양현정
Original Assignee
노을 주식회사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US201662298959P priority Critical
Priority to US62/298,959 priority
Priority to KR1020160069938A priority patent/KR20170099739A/en
Priority to KR10-2016-0069938 priority
Priority to KR1020160069937A priority patent/KR20170099738A/en
Priority to KR1020160069936A priority patent/KR20170099737A/en
Priority to KR10-2016-0069937 priority
Priority to KR10-2016-0069936 priority
Priority to KR10-2016-0095739 priority
Priority to KR1020160095739A priority patent/KR20170099741A/en
Priority to KR1020160118462A priority patent/KR20170099742A/en
Priority to KR10-2016-0118462 priority
Priority to KR1020160144551A priority patent/KR20170099745A/en
Priority to KR10-2016-0144551 priority
Application filed by 노을 주식회사 filed Critical 노을 주식회사
Priority to KR10-2017-0024391 priority
Priority to KR1020170024391A priority patent/KR20170099788A/en
Priority claimed from CA3015598A external-priority patent/CA3015598A1/en
Publication of WO2017146506A1 publication Critical patent/WO2017146506A1/en

Links

Images

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31Apparatus therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/49Blood
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/60Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances involving radioactive labelled substances

Abstract

The present invention relates to a blood staining patch, and a method and an apparatus for testing blood using same, and more specifically to a patch storing a staining reagent for staining blood and a method and an apparatus for economically testing blood by means of the patch. The method for testing blood according to one embodiment of the present invention is a blood testing method for testing blood by staining a target to be stained by means of the patch which has a networked structure with micro-cavities and which stores therein a staining reagent for staining a target to be stained in the blood, and comprises the steps of: placing blood in a reaction area; and transferring a staining reagent to the reaction area by means of the patch in which the staining reagent is stored.

Description

Patches of blood stained the blood test method and apparatus using the same

The present invention, as for the blood stained patch, blood test method and apparatus using the same, and more particularly, to store the dye sample to stain the blood, to a method and apparatus for inspecting the patch and economic blood by using this .

The increased desire for quality of life that is fast-paced with aging, such as diagnostics market oriented early diagnosis and early treatment of growth each year from around the world, including Korea and, it is quick and easy diagnostics is emerging as an important issue. In particular, in vitro diagnostic (IVD: In-vitro Diagnosis) and field diagnostics immediately diagnosed by the patient (POCT: point-of-care testing) going transition into a form that can perform diagnosis without using a large diagnostic equipment, such as a trend. On the other hand, one of the blood tests of the specific diagnostic field to perform an in vitro diagnosis, occupies a large proportion in the field of in vitro diagnosis, one of the most widely used diagnostic methods.

Blood tests, a branch of Hematology (hematology), mainly by examining the presence of bacteria in the blood or blood, such as the red blood cells, white blood cells and platelets in the blood types of ingredients are used to diagnose the health condition or disease, a disease of the patient.

Traditionally, a blood test is significantly examiner can be divided into an indirect test method, represented by such as direct examination of the way to observe the blood directly through the eye and flow cytometry (flow cytometry) or the impedance measurement method (electrical impedance measurement method) through a microscope is.

Direct test method is performed mainly by observing the staining results with the microscope after staining a blood smear on a slide glass using the dye (staining solution). Direct examination of the prior art method is the step of observing the process, the dyeing was visually blood through a microscope to stain the process, the blood smear to smear the blood will depend entirely on the hand of the examiner. Thus, the conventional method of direct examination, so as well as self-needed professional inspection requires a lot of time on the test may not depart from the shape made in the laboratory unit.

On the other hand, the indirect test method is micro channel (micro-fluidic channel) blood characteristics through an amount of change in impedance to examine the characteristics of the blood through the spectral light or a current applied to the blood sample by irradiating laser was passed through the blood to checks. Due to the above characteristics indirect test method is automated it is relatively made large hospitals, etc., but is utilized, because it does not directly observe the blood it is difficult that there is a technical limit scrutiny.

One object of the present invention is to provide a patch that can hold the material.

One object of the present invention is to provide a patch that can provide a reaction space of the material.

One object of the present invention is to provide a patch that can deliver a substance.

One object of the present invention is to provide a patch capable of absorbing material.

One object of the present invention is to provide a patch that can provide the environment.

One object of the present invention is to provide a patch for storing dye to dye the blood sample.

One object of the present invention is to provide a blood test method using the patch.

It is not the object of the present invention be limited to the above-described object, an object which is not referred to will be able to be clearly understood to those of ordinary skill in the art from the drawings herein and the appended claims.

According to one aspect of the invention, the dye for dyeing the dyed object in the blood sample; And a net structure for the dyed sample is provided in the net structure to form the micro-cavity that is stored, passing a portion of the stored stain sample contacts the reaction zone of the blood is positioned in the reaction region; dye patches containing the It can be provided.

According to another aspect of the invention, including a net structure to form the micro-cavity and use the patch to store dyed sample dyeing dye subject the blood to the minute voids, a blood test by the staining of the stained target to perform a blood test method, comprising: placing the blood into the reaction area; And the step of forwarding the sample dye in the reaction zone using the patch for storing the dyed sample; there is a blood test method comprising the can be provided.

According to still another aspect of the invention, including a net structure to form the micro-cavity and use the patch to store dyed sample dyeing dye subject the blood to the minute voids, a blood test by the staining of the stained target is a blood test apparatus, the reaction zone to perform a position and plate support for supporting a plate to be the blood located in said reaction zone; Patch control unit for controlling the relative position of the reaction zone of a patch to deliver the dye sample to the reaction area using the patch for storing the dyed sample; A blood test apparatus that includes may be provided; and the reaction detecting section for detecting the result of the dye in the target blood staining in order to check the blood.

Not necessarily the solving means of the present invention is not limited to the above-described solution, solving means which are not mentioned will be able to be clearly understood to those of ordinary skill in the art from the drawings that the present specification and the accompanying .

According to the invention it is possible to easily perform the storage, transmission, absorption of the material.

According to the present invention can provide a reaction zone of the material or to provide a desired environment in a target area.

According to the present invention, a blood test can be carried out more easily, the test results can be quickly obtained.

Further, according to the invention, it is possible to obtain a diagnosis with sufficient efficacy by using a small amount of blood.

Further, according to the invention, it is appropriately controlled delivery and absorption of the substance by using a patch can significantly reduce the amount of staining the sample required for diagnosis.

According to the invention it is possible to perform the diagnosis by detecting a plurality of targets at the same time, so that it is possible to perform the patient-specific diagnosis.

It is not an effect of the present invention is not limited to the above-described effects, effects which are not mentioned will be able to be clearly understood to those of ordinary skill in the art from the drawings herein and the appended claims.

1 is a detailed illustration of an example of a patch according to the present application.

2 is a detail showing an example of a patch according to the present application.

Figure 3 illustrates relative to providing the reaction space in one example of the feature of the patch according to the present application.

Figure 4 illustrates with respect to providing the reaction space by way of example one of the functions of the patch according to the present application.

5 is a diagram for conveying the material as one example of the feature of the patch according to the present application.

6 illustrates for conveying the material as one example of the feature of the patch according to the present application.

7 shows for conveying the material as one example of the feature of the patch according to the present application.

Figure 8 illustrates for conveying the material as one example of the feature of the patch according to the present application.

9 is a block diagram for conveying the material as one example of the feature of the patch according to the present application.

Figure 10 is a block diagram for conveying the material as one example of the feature of the patch according to the present application.

11 illustrates for conveying the material as one example of the feature of the patch according to the present application.

12 illustrates for conveying the material as one example of the feature of the patch according to the present application.

13 is a diagram for conveying the material as one example of the feature of the patch according to the present application.

Figure 14 shows with respect to the absorbing material as one example of the feature of the patch according to the present application.

15 shows with respect to the absorbing material as one example of the feature of the patch according to the present application.

16 shows with respect to the absorbing material as one example of the feature of the patch according to the present application.

Figure 17 illustrates with respect to the absorbing material as one example of the feature of the patch according to the present application.

Figure 18 illustrates with respect to the absorbing material as one example of the feature of the patch according to the present application.

Figure 19 shows with respect to the absorbing material as one example of the feature of the patch according to the present application.

20 shows with respect to the absorbing material as one example of the feature of the patch according to the present application.

Figure 21 shows with respect to the absorbing material as one example of the feature of the patch according to the present application.

Figure 22 shows with respect to the absorbing material as one example of the feature of the patch according to the present application.

Figure 23 shows with respect to providing the environment by way of example one of the functions of the patch according to the present application.

24 shows with respect to providing the environment by way of example one of the functions of the patch according to the present application.

25 shows with respect to providing the environment by way of example one of the functions of the patch according to the present application.

Figure 26 is a one embodiment of a patch according to the present application, shows a case of performing the absorption and transfer of the material.

Figure 27 is a one embodiment of a patch according to the present application, shows a case of performing the absorption and transfer of the material.

Figure 28 is a one embodiment of a patch according to the present application, shows a case of performing the absorption and transfer of the material.

Figure 29 is a one embodiment of a patch according to the present application, shows a case of performing the absorption and transfer of the material.

Figure 30 is a one embodiment of a patch according to the present application, shows a case of performing the absorption and transfer of the material.

Figure 31 is a one embodiment of a patch according to the present application, shows a case of performing the provision of the absorption, transmission and environment of the material.

Figure 32 shows the case of performing the provision of the absorption, transmission and environment, material as one embodiment of a patch according to the present application.

Figure 33 is a one embodiment of a patch according to the present application, illustrates one embodiment of a plurality of patches.

Figure 34 illustrates an embodiment of the plate with one embodiment of the patch according to this application, the plurality of patches and a plurality of target areas.

35 and 36 is a diagram according to an embodiment of the blood smear method according to an embodiment of the invention.

37 is a view of another example of the blood smear method according to an embodiment of the invention.

38 and 39 are views directed to imaging the stained blood in the embodiment;

Figure 40 shows a flow chart for explaining an example of the blood test method according to the present application.

Figure 41 shows a flow chart for explaining an example of the step of transferring the dye sample to the reaction zone of the methods blood test in accordance with one embodiment of the present application.

Figure 42 shows a flow chart for explaining another example of the step of transferring the dye sample to the reaction zone of the methods blood test in accordance with one embodiment of the present application.

Figure 43 shows a flow chart for explaining another example of the blood test method according to the present application.

Figure 44 shows a flow chart for explaining an example of the step of removing foreign material from the reaction zone of the blood test method according to another embodiment of the present application.

Figure 45 shows a flow chart for explaining another example of the blood test method according to the present application.

Figure 46 shows a flow chart for explaining an example of providing a predetermined reaction environment in the area of ​​the blood test method according to another embodiment of the present application.

Figure 47 shows a flow chart for explaining still another example of re-blood test method according to the present application.

Figure 48 shows a flowchart illustrating a blood test method according to the simple staining as an example of a blood test method according to the present application.

49 is a view showing a process of staining the sample is delivered by the blood test method according to the simple staining according to the present application.

Figure 50 to Figure 53 is a view relating to the image taken at a blood test method according to the simple staining according to the present application.

Figure 54 shows a flowchart illustrating a blood test method according to the Romanov ski dye as another example of the blood test method according to the present application.

55 is a view showing a process in which a first dyeing the sample is delivered by the blood test method according to the Romanov ski dyeing according to the present application.

56 is a view showing a process in which the second dye sample is delivered by the blood test method according to the Romanov ski dyeing according to the present application.

Figure 57 and Figure 58 is a diagram of the image imaged by the ski Romanov blood test method of the dye according to the present application.

59 is a view showing a process in which a first dye and a second sample dyed sample is passed along in the blood test method according to the Romanov ski dyeing according to the present application.

60 is a flowchart illustrating a blood test method according to the Gram stain as another example of the blood test method according to the present application.

Figure 61 to 63 is a view showing the present dyeing, mordanting, bleaching, dyeing process control in the blood test method according to the Romanov ski dyeing according to the present application, respectively.

Figure 64 illustrates one embodiment of a blood test apparatus according to the present application.

Figure 65 shows an example of a patch control according to one embodiment of the blood test apparatus according to the present application.

Embodiment described herein is intended for clearly illustrate the features of the present invention to those of ordinary skill in the art, not limited by the embodiment of the present invention described herein, the present invention range shall be construed as including a modification or variant which is not departing from the spirit of the invention.

As used herein, however, select a common term currently used widely as possible in consideration of the functions of the present invention vary depending on the appearance of the intended, customs or new skills of one of ordinary skill in the art can. However, when used to define the different specific terms this means there will be described in any separately as to the meaning of the term. Therefore, the terms used herein should be construed based on the contents throughout the first half of the real meaning and are used herein with the term, not the name of a simple term.

The accompanying drawings in the present specification is the shape shown in the drawings as to facilitate describing the present invention it may be shown exaggerated, as needed to aid the understanding of the present invention, the present invention is not limited by the drawings.

Present when the specific description of the configuration or function of the known according to the present invention in the specification that are determined to obscure the gist of the present invention, detailed description thereof will be omitted as necessary.

According to one aspect of the invention, the dye for dyeing the dyed object in the blood sample; And a net structure for the dyed sample is provided in the net structure to form the micro-cavity that is stored, passing a portion of the stored stain sample contacts the reaction zone of the blood is positioned in the reaction region; dye patches containing the It can be provided.

Here, the dye sample to stain the target stain may include a dye acid sample, at least one of the basic dye-dyed sample and the neutral sample.

Here, the sample is stained, the sample can comprise a fluorescent dye to the fluorescent dye to the destination.

Here, the target dyeing, the dyed sample, and comprises at least one of a blood cell, bacteria and parasites present in the blood can be dyed, at least one of the cytoplasm, nucleus, and granules of the target dye.

Here, the dyeing destination plurality, the dyed sample, may include a first staining the sample and the second dye sample to stain the second stain the target of the stain subject to during the dyeing destination dyeing a first dyeing the target .

According to another aspect of the invention, including a net structure to form the micro-cavity and use the patch to store dyed sample dyeing dye subject the blood to the minute voids, a blood test by the staining of the stained target to perform a blood test method, comprising: placing the blood into the reaction area; And the step of forwarding the sample dye in the reaction zone using the patch for storing the dyed sample; there is a blood test method comprising the can be provided.

Here, the step of obtaining an image relating to the stained the blood by passing the dyed sample may further include a.

Here, the object is stained obtaining the blood cells and the blood, the type of the blood cell based on the image information, the count information and morphological information, at least one of may further include a.

Here, on the basis of the obtained information CBC: performing the (CBC Complete Blood Count cell); can further comprise a.

Here, the dyeing target, and the parasite or bacteria in the blood, the parasite or information about the presence of bacteria whether or not, information about the type, number information and morphological information, comprising: obtaining at least one of; may further comprise a is.

Here, the peripheral smear blood on the basis of the obtained information, the method comprising: performing the (CBC Complete Blood Count cell); can further comprise a.

Here, placing the blood, and the blood may be methods for fixing to the plate, perform the sample by a method that smear on the plate or the sample in any of the method for fixing to spread on the plate.

Here, the step of forwarding the sample dye in the reaction zone using the patch, contacting the patch to the reaction zone so that the dyed sample is moved to the reaction zone; And separating from said reaction zone to said patch; may include, and the patch is the staining staining excess sample in the sample which have not reacted with the target dyeing is removed from the reaction zone when separated from said reaction zone.

Here, the step of absorbing foreign matter using a wash patch for storing the washing liquid remaining on the excess dye sample and the reaction zone from the reaction zone; a may further include.

Here, the step of transferring the dye sample to the reaction zone using the patch, using the first patch for storing the first staining the sample to stain any of the dyed object of the cytoplasm and nucleus in the reaction zone transferring the first staining the sample; And the step of the second pass stained sample to the reaction zone using a second patch for storing the second dye for staining the sample to the other of the cytoplasmic and nuclear staining of the target; may include.

Here, the method comprising: using a first buffer for storing the patch buffer solution providing a pH point of the reaction zone; a may further include.

Here, providing the indicated pH, at least one of the time after the step of transferring the first staining the sample with the second pass the dyeing time between the step of passing the sample and the second dye sample It can be carried out at the time.

Here, the dye patch, the first staining the sample and a second store dyed sample, and transferring the dye sample to the reaction zone using the patch to dye the nucleus of the dye subject to dye the cytoplasm of the stained target phase, and the dye patch may include the step of so stained with the cytoplasm and the nucleus of the target dye, the first dye and the samples pass the second dye sample to the reaction zone.

Here, after the step of transferring the first sample and the second dye staining a sample, the method comprising using a buffer to store the patch buffer solution providing a pH point of the reaction zone; a may further include.

According to still another aspect of the invention, including a net structure to form the micro-cavity and use the patch to store dyed sample dyeing dye subject the blood to the minute voids, a blood test by the staining of the stained target is a blood test apparatus, the reaction zone to perform a position and plate support for supporting a plate to be the blood located in said reaction zone; Patch control unit for controlling the relative position of the reaction zone of a patch to deliver the dye sample to the reaction area using the patch for storing the dyed sample; A blood test apparatus that includes may be provided; and the reaction detecting section for detecting the result of the dye in the target blood staining in order to check the blood.

1. Patch

Significance of the 1.1 patch

In the present application, and it discloses a patch to treat (manage) a liquid material.

The material of the liquid phase can refer to substances in the liquid state to a substance capable of flowing (flow).

Material of the liquid may be a material of a single component having the fluidity (liquidity). Alternatively, the material of the liquid may be a mixture comprising a plurality of material components.

When the material of the liquid material be of a single component, a material of the liquid may be a compound comprising a substance or a plurality of chemical elements consisting of single elements.

When the substance is a mixture of the liquid, some of the material of the plurality of component functions as a solvent, others can function as a solute. That is, the mixture may be a solution.

On the other hand, the material of the plurality of components constituting the mixture can be uniformly distributed. Alternatively, the mixture containing the substance of the plurality of components may be a uniformly mixed mixture.

Material of the plurality of components can comprise a material that is not dissolved in a solvent and the solvent nor uniformly distributed.

On the other hand, some of the material of the plurality of components may be unevenly distributed. The material unevenly distributed is also possible if it contains a particle component (particle component) to non-uniformly distributed in the solvent. At this time, the particle components of the non-uniform distribution can be a solid phase (solid phase).

For example, materials that can be processed by using the patch are: 1) a single-component liquid, 2) a solution, or 3) it may be a state of colloid, depending on the case 4), the solid particles uniform within the material of the other liquid distribution it may be the state.

Hereinafter, it will be described in detail for the patch according to the present application.

1.2 General nature of the patch

1.2.1 Configuration

Figures 1 to 2 are views showing an example of a patch according to the present application. Hereinafter will be described with respect to the patch of the present application with reference to 1 to FIG.

1, a patch (PA) according to the present application may include a net structure (NS) and the liquid material.

Here, the material of the liquid phase, may be considered by dividing the base material (BS) and the additive material (AS).

May also, the patch (PA) is a gel (gel type). The patch (PA) may be implemented in a gel structure is a net structure formed by combining the colloidal molecules.

Patches (PA) according to the present application may include a structural (NS) of the three-dimensional network as a structure for the handling of a substance (SB) of the liquid. Net structure (NS) may be a solid structure continuously distributed. It said netting structure (NS) is a plurality of fine threads (thread) may have a network structure of a tangled mesh. However, it is the net structure (NS), not limited to the form of the number of fine threads entangled network, may be implemented in the form of a matrix of an arbitrary three-dimensional multiple microstructures formed are connected. For example, the netting structure (NS) may cheil skeleton which comprises a plurality minute voids (micro-cavity). In other words, the net structure (NS) are capable of forming a plurality of minute voids (MC).

Figure 2 illustrates the structure of another patch to an embodiment of the present application. 2, the net structure of the patch (PA), may have a spongy structure (SS). At this time, the net structure of the sponge structure (SS) may comprise a plurality of fine holes (MH). Hereinafter, the definition that the micro-holes and micro-cavity (MC) can be used interchangeably with each other, unless otherwise noted, the micro-cavity (MC) includes a concept of the fine holes (MH).

In addition, a net structure (NS) is, may have a regular or irregular pattern. Furthermore, the net structure (NS) may include all having a regular pattern region and the irregular region having the pattern.

Density of the net structure (NS) Figure (density) may have a value within a predetermined range. Preferably, the predetermined range may be determined to the extent the shape of the material (SB) of the liquid trapped in the patch (PA) being held in the form corresponding to the patch (PA). The density may be defined as a mass ratio, a volume ratio such that the net structure (NS) share of fine enough to the patch of the net structure (NS).

Patches according to the present application, by having a network structure of a three-dimensional, it is possible to treat the material (SB) of the liquid.

Patches (PA) may contain a substance of liquid phase (SB), substance (SB) of the liquid contained in the patch (PA) according to the application form of the netting structure (NS) of the patch (PA) to have the flowability of the material (SB) of the liquid it can be restricted by.

Substance (SB) of the liquid can freely flow within the net structure (NS). In other words, the substance (SB) of the liquid phase, is located at the plurality of minute voids to form the said netting structure (NS). Between adjacent micro-cavity which may cause flow of material (SB) of the liquid. At this time, the material (SB) of the liquid phase, may be present in a form that penetrates the frame structure forming the net organization. In the hole (pore) of the nano-sized to penetrate the material (SB) of the liquid to the frame structure may be formed when:

Further, the input whether the liquid substance (SB) of the depending upon the molecular weight to the particle size of the material (SB) of the liquid trapped in the patch (PA) to the frame structure of the network structure can be determined. Is relatively large, the molecular weight matter trapped in the micro-cavity, is a small molecular weight relative to the material input to the frame structure of the micro-cavity and / or the net structure (NS) can be captured.

In this specification, as referring to the condition where a term that "capture (capture)", material (SB) of the liquid phase in the holes of the net structure (NS) to form a plurality of micro-cavities and / or the nano-sized you can define. In addition, to flow between the state material (SB) of the liquid is trapped in the patch (PA), as described above, substance (SB) of the liquid is the micro-cavity and / or of the nano-size holes It is defined to include a state in which.

Substance (SB) of the liquid can be considered by dividing the base material (BS) and the additive material (AS), as shown below.

The base material (BS) has, it may be a material in a liquid having a flowability (SB).

The additive material (AS) may be a substance having the fluidity of a mixture of the base material (BS). In other words, the base material (BS) may be daily for. The additive material (AS) may be a particle that is insoluble in the solvent or the solute to be dissolved in the solvent.

The base material (BS) can be a material that can flow from within the matrix to form the said netting structure (NS). On the other hand, the base material (BS) may be uniformly distributed in the net structure (NS), it may be distributed only in a part of the net structure (NS). The base material (BS) has, it may be a liquid having a single component.

The additive material (AS) is, it may be a base material (BS) and a mixed or melting material on the base material (BS). For example, the additive material (AS) is, can function as a solute to the base material (BS) as a solvent. The additive material (AS), can be uniformly distributed in the base material (BS).

The additive material (AS) can be fine particles, that is insoluble in the base material (BS). For example, the additive material (AS) is, may include fine particles such as colloidal molecules and microorganisms.

The additive material (AS) is, it may include particles larger than the minute voids by the net structure (NS) form. If the size of the micro-cavity is smaller than the size of the particles contained in the additive substance (AS), the fluidity of the additive material (AS) can be limited.

Further, according to one embodiment, the additive material (AS) is, it may contain components that are optionally included in the patch (PA).

On the other hand, the additive material (AS) is, in relation to the above-described base material (BS), not necessarily all thirteen, or quantitatively, it means a material in a functionally inferior.

In the following, characteristics of the liquid substance (SB) trapped in the patch (PA) can be considered to be characteristic of the patch (PA). That is, the properties (characteristics) of the patch (PA) may depend on the nature of the entrapped material to the patch (PA).

1.2.2 characteristic (characteristic)

Patches (PA) according to the present application may include a net structure (NS), as described above. The patch (PA) may be by the net structure (NS) to treat the material (SB) of the liquid. The patch (PA), the material (SB) of the liquid that is trapped in the patch (PA) can be maintained to at least some of the unique characteristics.

For example, it can lead to the spread of material in the area of ​​the patch (PA), which substance (SB) of the liquid is distributed, it can act a force such as surface tension.

The patch (PA) may provide a fluid environment so that the target material spread by thermal motion, density or concentration difference of the material. Generally it referred to the "spread" also is to particles that make up the material by the difference in density means that the concentration is out of the concentration spread toward the low side, high. This diffusion phenomenon can be understood as a default, the resulting phenomenon is caused by the (translational motion, vibrational motion in the solid state such as in a gas or liquid) motion of molecules. In the present application, the term "diffusion", in addition to referring to the concentration or the developing concentration is spreading at a low place where particles of high density by the difference in density, of the molecule that the concentration occurs in a uniform state to each other it shall refer even the phenomenon of moving particles due to irregular movement. Also, to use as a free expression "irregular movement" of the particle, a special mention, 'spread' in the same sense. The substance in which the diffusion may be a solute that is dissolved in the material (SB) of the liquid, the solute may be provided in solid, liquid or gaseous state.

More specifically, the material unevenly distributed in the patch material of the liquid is trapped by the (PA) (SB) can be diffused in the space provided by the patch (PA). In other words, the additive material (AS) can spread in the space defined by the patch (PA).

The patch material (SB) material or the additive material (AS) which non-uniformly distributed in the liquid phase to (PA) is responsible for the spread in the micro-cavity is provided by the net structure (NS) of the patch (PA) can do. In addition, the region where the material or the addition of substances (AS) to the non-uniform distribution can be spread is that the patch (PA) and other materials being in contact or connection can be changed.

The concentration of the substance or the additive material (AS) to the non-uniform distribution of the said patch (PA) within a or said patch After a diffusion in the outer region that is associated with (PA), the material or the addition of substances (AS) is, the material or the addition of substances (aS) can be moved continuously by the random motion of molecules in the outer zone associated with the inner and / or the patch (PA) of the patch (PA) after the uniformly.

The patch (PA) may be implemented to exhibit the properties of hydrophilic or hydrophobic. In other words, the net structure (NS) of the patch (PA) may have the nature of the hydrophilic or hydrophobic.

If the nature of the netting structure (NS) with said liquid substance (SB) of a similar, wherein the net structure (NS) may be more effective to treat the material (SB) of the liquid.

Properties of the base material (BS) is hydrophilic or is exhibiting polarity, it may be a hydrophobic material that does not exhibit polarity. In addition, the nature of the additive material (AS) may be hydrophilic or hydrophobic.

Properties of materials (SB) of the liquid may be associated with said base material (BS) and / or the additive material (AS). For example, the base material (BS) and, if the additive material (AS) is all hydrophilic, the material (SB) of the liquid may be a hydrophilic, wherein the base material (BS) and the additive material (AS) both If hydrophobic, substance (SB) of the liquid may be hydrophobic. When the polarity of the added substance (AS) and the base material (BS) of different, materials (SB) of the liquid may be a hydrophilic or may be hydrophobic.

Between the net when both the polarity of the structural (NS) and the polar liquid substance (SB) of the hydrophilic or hydrophobic, or the net structure (NS) and the substance (SB) of said liquid may serve the personnel. When the polarity of the net structure (NS) with said liquid substance (SB) of the opposite, for example, the polarity of the net structure (NS) hydrophobic and if the substance (SB) of the liquid phase is tinged hydrophilicity between the net structure (NS) and the substance (SB) of said liquid may serve this repulsion.

Based on the above properties, the patch (PA) can be used to induce the desired reaction with a singly, a plurality, or other medium (medium). Hereinafter, a description is made of the functional side of the patch (PA).

However, in the following, for convenience of description, the patch (PA) is assumed to be a gel merchants that may be included in the hydrophilic solution. In other words, the net structure (NS) of the patch (PA), if not otherwise specified, will be described on the assumption that in the nature of hydrophilic.

However, the designed hayeoseoneun interpret the scope of the present application defined by the patch (PA) on the gel having the properties of hydrophilicity, in addition to the gel containing the solution stand for hydrophobic nature of the patch (PA), the solvent is a patch on the removed gel (PA), and that is of course if it is possible to implement a number of functions in accordance with the present application have a patch (PA) scope on the far sol.

2. The functions of the patch

Patches according to the present application, due to the above-described characteristics, may have a number of useful features. In other words, the patch is occupied by the material of the liquid (SB), it may be involved in the behavior of the materials (SB) of the liquid.

Accordingly, the less the patch (PA) reservoir functions, the status of the material is defined in a predetermined region of which forms the said patch (PA) according to the behavior aspects of the material from between the relationship and the patch (PA) including the outer region divided into a channeling function looks at the status of the material is defined.

2.1 reservoirs (Reservoir)

2.1.1 Significance

Patches (PA) according to the present application, it is possible to take the substance (SB) of the liquid, as described above. In other words, the patch (PA) may perform the function of a reservoir.

The patch (PA) may be captured (capture) the material of the liquid (SB) to a plurality of minute voids formed in the net structure (NS) through the net structure (NS). Substance (SB) of the liquid is the patch (PA) 3-dimensional network structure (NS) occupying at least a portion of the micro-cavity formed by the, or the net structure (NS) of the nano-sized hole (pore) formed on the back of It can penetrate.

Substance (SB) of the liquid positioned in the patch (PA) is, even if the distribution of the plurality of micro-cavity, does not lose the properties of the liquid. That is, the substance (SB) of the liquid is patch (PA) also has the flexibility, the material (SB) of the liquid distributed in the patches, (PA) may lead to diffusion of the material, a suitable solute to the substance to be dissolved is.

It will now be described in more detail with respect to the reservoir function of the patch (PA).

2.1.2 Storage (contain)

In this application patch (PA) is capable of capturing a target substance by the above-described characteristics. The patch (PA) may have a resistance within a certain range with respect to changes in the external environment. This end, the patch (PA) through can be kept in captivity for the material state. Substance (SB) of the liquid to be subjected to the trapping can occupy the net structure (NS) of the three-dimensional.

Will now be a function of the patch (PA) as described above is called convenience stores.

However, the frame structure forming the patch (PA) that is, one and / or the net structure (NS) in which the liquid material is stored in a space formed by the network structure means the end that stores the liquid material It is defined as covering both that at which the liquid substance storage.

The patch (PA) is capable of storing a liquid substance (SB). For example, by the force which acts in relation to the patch (PA) the net structure (NS) with said liquid substance (SB) of the patch (PA) is capable of storing the materials of the liquid (SB). Substance (SB) of the liquid can be stored in combination with the net structure (NS) by more than a certain intensity personnel.

The nature of the material of the liquid (SB) stored in the patch (PA) can be distinguished according to the nature of the patch (PA). More specifically, the patch (PA) if exhibits the properties of hydrophilicity, in combination with the general material of the liquid (SB) of the hydrophilic having polar, the three-dimensional material (SB) of the hydrophilic liquid fine It can be stored in the cavity. Or, if the patch (PA) that exhibits the properties of the hydrophobic, can store the substance (SB) of the hydrophobic liquid in the micro-cavity of the three-dimensional network structure (NS).

In addition, the amount of material that can be stored in the patch (PA), may be proportional to a percentage in volume of the patch (PA). That is, That is, it is possible to the amount of material to be stored in the patch (PA) proportional to a percentage to the amount of three-dimensional network structure (NS) of a support body that contribute to the shape of the patch (PA). However, of the material that can store the amount by volume between said patch (PA) are not necessarily having a predetermined proportional constant, the volume of the volume and the patch (PA) of the material that can be saved according to a design or manufacturing method of the net structure relationship may vary.

With the passage of the amount of material stored in the patch (PA) time can be reduced by evaporation or the like, falling off. In addition, it is possible to put the additional material to the patch (PA) to increase or maintain the amount of material stored in the patch (PA). For example, the patch (PA) can have a moisture preservatives for suppressing evaporation of water is added.

The patch (PA), can be implemented in a form to facilitate storage of the substance (SB) of the liquid. This means that in the case where the material that is affected by the humidity, light intensity, temperature environment, wherein the patch (PA) in order to minimize the denaturation of the material may be implemented. For example, in order to prevent the patch (PA) is to be modified by external factors such as bacteria, said patch (PA) can be treated with inhibitors, such as bacteria.

On the other hand, the patch (PA) has a material (SB) of liquid having a plurality of components can be stored. At this time, the material of the plurality of components, prior to the reference time or position with the patch (PA), the material being fed to the primary and the priority stored in the patch (PA) a predetermined time in the past since the secondary to the patch (PA) it is also possible in which the substance is stored. For example, when the material of the liquid (SB) of the two components in the patch (PA) storage, or the two components in the manufacture of the patch (PA) is stored in the patch (PA), in the manufacture of the patch (PA) , only one component will be able to be stored in the patch (PA) and later the other is stored, the two components after the production of the patch (PA) stored in sequence.

In addition, the material stored within the patch (PA) is, as described above, can exhibit fluidity by default, and may also be an irregular movement to spread motion caused by the molecular movement within said patch (PA).

Service 2.1.3 reaction space (space)

3 and 4 are drawings illustrating to provide a reaction space in one example of the feature of the patch according to the present application.

As shown in Figure 3 and 4, the patch (PA) according to the present application may perform the function of providing space. In other words, the patch (PA) may provide a space for the substance (SB) of the liquid to travel through the space constituting the space and / or the net structure (NS) formed by said netting structure (NS) is.

The patch (PA) is capable of providing space for the non-diffusion and / or random movement of the particles of the active particles (referred to as activities hereinafter non-spread). Activity other than the diffusion is, may indicate a chemical reaction, but not limited thereto may refer to the physical state change. More specifically, the activity is, solutes or particles comprising a specific binding reaction, said material between the components included in the chemical reaction, the material is the chemical composition of the material is changed before and after the activity is unevenly distributed in the non-diffusion the uniform, aggregation of some of the components contained in the material, or may include a biological activity of the material part.

On the other hand, if the plurality of materials involved in the activity, a plurality of materials may be co-located with the patch (PA) before the reference point. It said plurality of materials, may be added sequentially.

By changing the environmental conditions of the patch (PA), it is possible to increase the efficiency of the function to provide a space for activities other than the diffusion of the patch (PA). For example, it is possible to vary the temperature of the patch (PA), or in addition to the electrical conditions that promote the activity or induce the onset of activity.

According to Figures 3 and 4, the first material (SB1) and a second material (SB2) located on said patch (PA) is or modified as a third material (SB3) by reaction inside the patch (PA), the it is possible to produce a third material (SB3).

2.2 channel (channel)

2.2.1 Significance

The patch may result in movement of the material between the (PA) and the outer region. Furthermore, the patch or the material in the outer region of the patch (PA) moves from (PA), is a substance in the patch (PA) can be moved from the outer region.

The patch (PA) may form a movement path of the material or be involved in the movement of the material. More specifically, the patch (PA) is outside the material through the material of the liquid (SB) trapped in participating in the mobile or the patch (PA) of the material of the liquid (SB) trapped in the patch (PA) It may be involved in the move. From the patch (PA) of the base material (BS) or the additive material (AS) is missing or out of, a foreign substance can be introduced into the patch (PA) from the outside area.

The patch (PA) is capable of providing the functionality of the moving passage of the material. That is, the patch (PA) may be involved in the movement of the material to provide a channel function of the mass transfer. The patch (PA), can, due to the unique properties which the material (SB) of the liquid to provide a passage (channel) of the mass transfer.

The patch (PA), the material of the liquid in the gap between the outer region and, depending on whether the connection between itself and the outer area the movement of the material (SB) in the liquid state or the outer area (SB ) it may have a non-moving state of the. In addition, when the patch (PA) and the channeling (channeling) in the area between the outer discloses the patch (PA) it may have a specific function.

In the following, first, description will be given to the disabled state, the movement of the material and the movement of the material state and, in the patch (PA) is performing a specific function, and whether or not the patch (PA) and the connection of the outer region linkage will be described in detail.

Basically, between it said patch (PA) outside the region, the primary reason for the movement of the material (SB) of the liquid occurs due to the irregular movement and / or diffusion of the material. However, in order to control the movement of material between the outer region and the patch (PA), to control the external environmental factor (e. G., Control of the thermal conditions, including control of the electrical conditions) is possible is already described is.

2.2.2 a movable state (movable state)

In a state capable of moving the material to flow between the liquid phase of a substance (SB) and / or the material located outside the trapping region to said patch (PA) may occur. In a state as possible, the material is moved between the material of the trapped liquid in the patch (PA) (SB) and the outer region can result in movement (move) of the material.

For example, it is in the state capable of moving the material to move this portion of the liquid component of the material (SB) or the liquid substance (SB) of the by diffusion or irregular motion in the outer region. Or, it may be in a state as possible, the material is moving, some components of the outer material or the outer material is located at said exterior region to move by diffusion of a material of the liquid (SB) or irregular movement of the patch (PA).

The substance is a movable state may be caused by the contact. The contact is, may indicate that the substance (SB) of the liquid trapped in the patch (PA) is connected with the outer region. It said contact means, may mean that the flow area of ​​the material (SB) of the liquid is at least partially overlapped with the outer region. The contact is, may indicate that the external material is connected to at least a portion of the patch (PA). The material is a movable state, the substance (SB) of the trapped liquid can be understood to be a flow range expanded. In other words, in a state capable of moving the material, and the liquid it may be expanded to include at least a portion of the outer region of the flowable range of the material (SB) of the liquid trapped in the material. For example, when the substance (SB) of the liquid is in contact with the outer region, the material (SB) the flowable range of the entrapped liquid can be expanded to include at least a portion of the contact outside the area. If more detail, the outer region of the outer plate, the material (SB) of the liquid phase is a flowable region may be expanded to include a region in contact with the material (SB) of the liquid in the outer plate.

2.2.3 movement impossible state (immovable state)

In the state the substance is not possible movement of the patch material of the liquid (SB) and move (move) of material between the outer region trapped in (PA) can not occur. However, that the patch, the material of the liquid (SB) and a foreign object located in the outer region on each captured (PA) may cause movement of the material, of course.

State the material can not be moved may be a state where the contact is released. In other words, the patch (PA) is in the state where the contact of the outer region is turned off in the material of the liquid (SB) and the external region and the foreign matter remaining on the patch (PA) the movement of the material is no longer available .

More specifically, the contact is disengaged may substance (SB) of the liquid trapped in the patch (PA) This means a state that is not connected to the external region. State where the contact is released may substance (SB) of the liquid phase means a state that is not associated with a foreign object located in the outer region. For example, the state where the movement of the material is impossible may be induced by becoming a said patch (PA) and the outer separation region.

The "movable state" defined herein may be caused by a transition between states such as "Move impossible" gajina meaning distinct from, over time, changes in the environment. In other words, the patch and (PA) can be a possible condition was the non-mobile moving state, may be capable of a movement impossible state was moving state, a state wherein it was possible the patches (PA) moving state is not possible movement after the can will also be moved back to the ready state.

2.2.4 Classification of functions

2.2.4.1 transfer

In this application, the patches (PA), can pass by at least the outer region of interest some of the due to the aforementioned characteristics, the patch material of the liquid occupying the (PA) (SB). Transfer of the material can as a certain condition is satisfied means that a portion of the material of the liquid (SB) trapped in the patch (PA) is separated (separate) from the patch (PA). What is material (SB) of said liquid phase is partially separated, this may mean that some of the material that is patch (PA) or of the region extracted from the impact of (extracted) radiation (emitted) or liberated (released). This is because the above-described concept of a sub-channel function of the patch (PA), can be construed as defined by the patch (PA) being transmitted to the outside (delivery) of the material located on said patch (PA).

The purpose of the outer region may be a different patch (PA), a drying area, or a liquid region.

The predetermined condition for the transmission is generated, can be determined by temperature change, pressure change, change in electrical characteristics, environmental conditions such as the physical state change. For example, the chemical and the patch (PA) wherein the patch (PA) material (SB) of the liquid when the substance (SB) and the binding force of the liquid phase than the net structure (NS) in contact with a strong object is the contact an object of by being able to be combined, and as a result, at least a portion of the material (SB) of the liquid can be delivered to the object.

Hereinafter referred to as a transfer function for the sake of convenience of the patch (PA) as described above.

The transfer, the material (SB) of the liquid is moved between the patch (PA) and the substance (SB) of the liquid is moved between the outer area available (movable) state and said outer region and said patch (PA) through the unavailable state (via / through) may occur.

More specifically, when the substance (SB) of the liquid phase is the movable state, it can be spread between the outer region and the patch (PA), or it may be by the irregular movement to move to the outer region. In other words, the base solution and / or adding substance (AS) contained in the material (SB) of the liquid can be moved in the patch (PA) in the outer region. In the state material (SB) of the liquid that does not accept the movement, the movement between the outer region and the patch (PA) is not possible. In other words, because, due to diffusion and / or an irregular motion of the material (SB) of the liquid portion of the material was moved to the outer area from the patch (PA) is in the movable state in transition to the mobile impossible , it can not be moved back to the patch (PA). Accordingly, some of the material (SB) of the liquid may be delivered to some of the outer region.

The transfer may be performed according to a difference between the attraction between the liquid substance (SB) and the netting material of the force and the liquid between the structural (NS) (SB) and said outer region and the outer material. The force can be caused from the affinity or specificity of the polar binding relationship.

Than non-specific, and the material (SB) of the liquid phase is hydrophilic, the case as compared to the net structure (NS) of the patch (PA) is the outer region and the outer material more hydrophilic property is strong, the movable state and the moving the through the state at least some of the material of the liquid (SB) which has been trapped in the patch (PA) may be delivered to the outer area.

Delivery of a substance (SB) of the liquid may also be performed selectively. For example, some of the component and the case where the specific binding relationship between the outer material is present, said part components via a state in which the substance is a movable state and the moving of the material can not be contained in the material (SB) of the liquid has a selective transfer can be generated.

More specifically, the patch (PA) is assuming the case of passing the material to the outside plate (PL) of the flat plate form, a portion of the patch material of the trapped liquid in (PA) (SB) (e.g., solute this part of) the substance specifically binding to the may be applied to the outer plate (PL). The plate in this instance, the patch (PA) is the movable state and the patch (PA) a portion of the solute that binds via the immovable state, specifically as a coating material to the outer plate (PL) red ( by PL) it can optionally pass.

According to the following, some examples of the different region in which the material is moved, will be described as the transfer function of the patch (PA). However, it is the concept of "transfer" in the "release" and the material (SB) of the liquid substance (SB) of the liquid to be mixed as the specific description.

Here, a description will be given of a case in which the patch (PA) in the material (SB) of the liquid phase is passed to a separate, external plate (PL). For example, consider a case in which the moving material in the patch (PA) to the plates (PL), such as a slide glass.

The patch (PA) and the plate (PL) is in contact As according to the patch (PA) material (SB) of the liquid that has been captured by the mobile at least spreading to a portion of the plate (PL), or moved by the irregular movement can. The patch (PA) and when the contact of the plate (PL) separated, some materials (i.e., some of the material (SB) of the liquid) is the patch from the patch (PA) that was moved to the plate (PL) ( can not be moved back to the PA). As a result, there can be a part of the material delivered to the plate (PL) from said patch (PA). At this time, some of the material to be delivered can be, the additive material (AS). In order by the contact and separation to materials "pass" in the patch (PA), to be a human and / or binding force acting between the material and the plate (PL) present, and the force and / or the bonding force is the It should be larger than the force acting between the material and the patch (PA). Therefore, when the above-mentioned 'transmission condition "is not satisfied, transfer of material between said patch (PA) and the plate (PL) may not be generated.

Further, by providing a temperature or electrical condition to the patch (PA) it is possible to control the delivery of a substance.

In the patch (PA) material moving to the plate (PL), it can be dependent on the contact area of ​​the patch (PA) and the plate (PL). For example, the patch has a mass-transfer efficiency of the patch (PA) and the plate (PL) can be increased or decreased in accordance with the area of ​​the (PA) and the plate (PL) contact.

If the patch (PA) comprises a plurality of components, only some components can be selectively moved out of the plate (PL). More specifically, the outer plate (PL) can have a substance that specifically binds with some components of the plurality of components are fixed. At this time, the material fixed to the outer plate (PL) can be fixed to the liquid or may be solid state, in the separate zones. In this case, the patch (PA) and a contact such as the separate areas and some material out of the plurality of components to form a specific binding go to the plate (PL), said patch (PA) the plate (PL ) in case that it is separate from, and not all components can be selectively released by the plate (PL).

Figure 5 to 7 are shown as one example of the transfer material of the functions of the patch (PA) according to the present application, the delivery of material to the outer plate (PL) from said patch (PA). According to Fig. 5 to 7, wherein the patch (PA) may deliver a portion of the material stored in the patch (PA) to the plates (PL) by contact with the outer plate (PL). At this time, it is to forward the material, the movement of the material can be accomplished by contact with the plate. At this time, and the water film (WF) to the contact surface near to the plate and the patch (PA) in contact can be formed, the movement of the material can be made available through the formed water film (WF).

Here, a description will be given of the case of a material (SL) having a fluid from the patch (PA) is a transmitter (SB) of the liquid. Here, the material (SL) has a fluidity, and may be a liquid material in a flowing or contained in a separate storage space state.

The patch (PA) and the fluidity material is in contact with (e.g., added to the patch (PA) in the solution) substance (SB) of the liquid that has been trapped in the patch (PA) as at least a part of the flow having been spread with material (SL) can be moved, or moved by the irregular movement. The patch (PA), and when the fluidity material is separated in, not part of material (SB) of the liquid that was moved from the patch (PA) of a material in which the flow is again moved to the patch (PA) being , there are some materials that were in the patch (PA) to be transmitted to the material in which the fluidity.

Mass transfer between the patch (PA) and the material (SL) having the fluidity, may depend on the contact area of ​​the patch (PA) and the material (SL) having the fluidity. For example, in accordance with (D For example, the patch (PA) is to be input or the like solution) The patch (PA) and the area of ​​the contact material (SL) with the fluid, with said patch (PA) and the flow mass transfer efficiency of the substance (SL) may be increased or decreased.

In addition, mass transfer between the patch (PA) and the material (SL) having the fluidity may be controlled through the physical separation of material in which the fluidity and the patch (PA).

And the distribution density of the additive material (AS) of the material (SB) of the liquid phase and the distribution density of the additive material (AS) of the material in which the mobility, of a material in which the flow from the patch (PA) the additive material may be (AS) is delivered.

However, the patch (PA) is a material (SL) with the fluid in in delivering the substance (SB) of the liquid, the patch, a physical separation between the (PA) and the material (SL) having the fluidity is necessarily Is not. For example, if the patch force (driving force / causal force) that cause mass transfer in the liquid having the flow from (PA) to be lost or is reduced below the reference value, the movement of the material to fail.

In the 'Forward' between the patches (PA) and the material (SL) with the fluid, is not required is "delivery condition" between the above-mentioned the patch (PA) and the material (SL) having the fluidity may. This material has already moved from a material (SL) having a fluidity are are moved by diffusion and / or random movement within the material (SL) with the fluid, material that the movement by the mobile and the patch (PA) When the distance between and away over a certain distance the material can be understood as a transfer of a material (SL) having the fluidity. Since it is, the movable range is very limited range, which is extended by the contact if the plate (PL), the attraction between the patch (PA) with the material to move to the plate (PL) can significantly act so, but, in the relationship between the material and the patch (PA) having the fluidity, because the patch (PA) and the movable range is extended by the contact of the plate (PL) is relatively much wider range, the fluidity is a substance with a movement of a material (SL) with attraction between the patch (PA) is because it is meaningless.

8 to 10 illustrates the one example of the transfer of the material of the functions of the patch (PA) according to the present application, the delivery of a substance to which the liquid material from the patch (PA). Referring to Figure 8 to 10, wherein the patch (PA) may deliver a portion of the material stored in the patch (PA) of a material with an external fluid. Passing a portion of the stored material of the patch (PA) is in the fluid is introduced to the material in contact with, said patch (PA) the material of the liquid (SB) and the materials each material in which the liquid trapped in the It may be formed by being had to the movement state.

Here, assume a case of moving the material to another patch (PA) from the patch (PA). In the contact region in which the other patch (PA) and the patch (PA) contact material (SB) of the liquid provided to the patch (PA) may be moved at least in a portion of the other patch (PA).

In the contact region, the material of liquid phase (SB) provided on each of the patches (PA) have been spread with different patch (PA) (the other patch) can be moved. At this time, due to the movement of the material, the concentration of the substance in liquid phase (SB) provided on each of the patches (PA) may vary. Also in this embodiment, as described above, the patch (PA) and the other patch (PA) may be separated at this time, some of the other patch (PA of the liquid material of the patch (PA) (SB) ) it can be delivered to.

Mass transfer between the patch (PA) and the other patch (PA) can be carried out by a change in environmental conditions comprises a physical change of state.

Mass transfer between the patch (PA) and the other patch (PA) (another patch), can be dependent on the contact area of ​​the patch (PA) and the other patch (PA). For example, according to the area of ​​the patch (PA) and the other patch (PA) are in contact, the mass transfer efficiency between the patch (PA) and the other patch (PA) can be increased or decreased.

Figure 11 through 13 shows the delivery of a substance into the patch as an example of delivery of a substance of the functions of (PA), the patch other patches (PA2) from (PA1) of the present application. Referring to Figure 11 to 13, wherein the patch (PA1) may deliver a portion of the material stored in the patch (PA1) to other patches (PA2). Passing a portion of said material is entrapped material to the patch (PA1) is in contact with the other patch (PA2), the patch material of the trapped liquid in the (PA1) (SB) and the other patch (PA2) It may be formed by being had to the flow as possible with each other.

2.2.4.2 Absorption

Prior to the description, the "absorption" of the functions of the patch (PA) according to the present application may be treated similarly in the "transmission" mentioned above, in some embodiments. For example, when assuming the movement of the material due to the difference in density of the material, by varying the concentration of the substance (SB) of the liquid, in particular the concentration of the additive material (AS), to control the direction of movement of the moved material it may have a common aspect in this regard. In addition, the patch (PA) may also be common, etc. Similarly, movement control, and selective absorption of substances through the separation of the physical contact and of which will be clearly understood to those skilled in the Field of the present application.

Patches (PA) according to the present application, it is possible to capture, foreign substances by the above-described characteristics. The patch (PA) may be, an incoming (pull) the foreign matter existing outside the area defined by said patch (PA) to the area in which the functional effect of the patch (PA). The pulling the outer material can be captured as material (SB) of the liquid of the patch (PA). The inlet to the outside material, it is possible to result from said patch (PA) the attraction between the material of the liquid (SB) and the foreign matter trapped in the group. Alternatively, it is incoming to the outside material, it is possible to result from the attraction between the region and the external substance which is not occupied by the substance (SB) of the liquid of the net structure (NS). Pulling of the outer material can, resulting from the force of the surface tension.

Hereinafter referred to as the functionality of the patch (PA) as described above for convenience absorption. The absorption can be construed as defining the migration to a lower concept of the channel functions of the above-mentioned patch (PA), the patch of the outer material (PA).

The absorption, the patches are (PA) may cause the movement through the possible states and the non-moving state of the material (via / through) of the material.

Materials that said patch (PA) that can be absorbed can be a liquid or solid state. For example, the patch (PA) is in the solid state contained in the liquid substance (SB) and the foreign matter which is located on said patch (PA) when in contact with an external substance containing the substance of a solid state material and to the human it can be performed by the absorption of the material. As another example, the patch may (PA) can be carried out in the case which is in contact with foreign matter in the liquid phase, binding of the liquid phase of a substance (SB) and the foreign matter in a liquid which is located in the patch (PA).

The foreign material absorbed in the patches (PA), through the micro-cavity of the patch mesh structure (NS) constituting the (PA) moves to the inside of the patch (PA), or distributed on the surface of the patch (PA) can do. Distribution position of the foreign object can be determined from the magnitude of naejineun molecular weight of the foreign matter particles.

The shape of the patch (PA) may be modified while the absorption takes place. For example, it is possible to change the volume, color, etc. of the patch (PA). On the other hand, it is possible in addition to the external conditions, such as while the absorption takes place in said patch (PA), the temperature change in the absorption environment of the patch (PA), to change the physical state activation or slow down the absorption of the patch (PA).

If less, the absorption taking place, according to some examples of the outer region to provide a material that is absorbed into the patch (PA), will be described with respect to the absorption as a function of the patch (PA).

Hereinafter, it is assumed a case in which the patch (PA) is absorbed to the outer material from a separate, external plate (PL). Here, there may be mentioned the need for external substrate includes a plate (PL), such as in, but does not absorb the external material is a foreign object can be located.

It said outer plate (PL) may be the coating material. In particular, the plate (PL), it may be the material is applied in powder form. Material that is applied to the plate (PL) may be a mixture of multiple components or a single component

It said plate (PL) is, may have a flat plate shape. In addition, the plate (PL) is a type that can be modified in order to improve storability, etc. of the material. For example, to improve the contact between with the patch (PA) using the well (well) modified or patterned plate (PL) to the surface of improving the storability reduce or, as negative or positive plate (PL) to form a may.

The patch (PA) according to the present application is to absorb the substance from the plate (PL), may be of the contact of the plate (PL) and the patch (PA). At this time, in the contact area of ​​the contact surface near between the plate (PL) and the patch (PA), the water film due to a substance applied to the patch (PA) material in the trapped liquid (SB) and / or the plate (PL) on (WF) can be formed. When the water film (WF, aquaplane) is formed in the contact region, the material that has been applied to the plate (PL) it can be captured in the water film (WF). The substance entrapped in the water film (WF) can freely flow within the patch (PA).

In the case where the patch (PA) that are spaced apart from the plate (PL) and a predetermined distance above the separation, the material the water film (WF) is that is applied to the plate (PL) by moving hooked to the patch (PA) the patch It can be absorbed by (PA). Material that has been applied to the plate (PL) is, as the patch (PA) the plate (PL) and a predetermined distance or more apart, and can be absorbed into the patch (PA). The patch (PA) and the plate (PL) is When spaced apart, the material (SB) of the liquid provided to the patch (PA) is unsubstituted or movement to the plate (PL), wherein the patch only the amount of minor degree (PA ) it can be absorbed into.

All or part of the material that is applied to the plate (PL) can be reacted specifically with all or part of the materials that are trapped in the patch (PA). In this connection, it is to the patch (PA) is absorbed material from the separate plate (PL), it can be carried out selectively. In particular, the patch wherein the patch (PA) than the plate (PL) with respect to the portion of the material which is trapped in (PA) that can geureohal when having a stronger force.

For example, there may be some material fixed to the plate (PL). In other words, the part material is fixed to the plate (PL), and may be applied to some materials have not been fixed or fluid. At this time, when the patch (PA) and the plate (PL) is in contact and separated, only the material except for the part material fixing of the material applied to the plate (PL) may optionally be as to be absorbed into the patch (PA). Alternatively, it is also possible the selective absorption occurring due to the polarity of the entrapped material to the plate (PL) the material and the patch (PA) located on, regardless of the fixed or not.

For example different materials (SB) of the liquid trapped in the patch (PA) is in the case of combining at least some of the specific of the material applied to the plate (PL), said patch (PA) is the plate ( PL) if the material was in contact with the separation in the coated, at least partially bond to the material applied to the plate (PL) to the specific it can be absorbed into the patch (PA).

Another example one, some of the material applied to the plate (PL) can be reacted specifically with a pre-fixed to the plate (PL) materials ever. In this case, it can be absorbed only with the exception of the material responsive to the plate (PL) as a specific and pre-fixed to the plate (PL) of a coating material as the material ever patch (PA).

Figure 14 to 16 shows that the absorption material from the one example of the absorption of the material of the functions of the patch (PA) according to the present application, the patch (PA) outside the plate (PL). Referring to Figure 14 to 16, wherein the patch (PA) is capable of absorbing a portion of the material located on the outer plate (PL) from the outside of the plate (PL). The absorbing said substances, said patch (PA) is the by contact with the outer plate (PL) water film (WF) in the contact region near the outer plate (PL) and the patch (PA) is formed, the water film ( the material through the WF) can be made by being movable by said patch (PA).

Here, assume a case from a material (SL) having a fluidity in which the substance is absorbed into the patch (PA). As material (SL) having a flow box, the liquid may be a foreign matter in a flowing state or contained in a separate storage space. More specifically, the portion of the material (SL) and the being of the trapped liquid in the patch (PA) material (SB) have the environment in which the cross-flow, the material (SL) having the flow having the flow or It may be all that is absorbed into the patch (PA). At this time, the environment in which the cross flow may be formed by at least a portion in contact with the material (SL) the patch (PA) having the fluidity is.

The patch (PA) whereby the patch (PA) is in contact with the material (SL) with the fluid may be a state capable of moving the material (SL) and the substance having the fluidity. When the patch (PA) is separated from the material (SL) having at least a portion of the fluid material (SL) with the fluid it may be absorbed into the patch (PA).

The material from (SL) with the fluid in which the substance is absorbed into the patch (PA), may depend on the concentration difference of material (SL) with the material and the fluid trapped in the patch (PA). In other words, the material (SL) having the fluidity than having a concentration for a given additive material (AS), the patch (PA) material (SB) is the given additive material (AS) of the liquid trapped in the when the concentration with respect low, the additive material (aS) of the predetermined number to be absorbed into the patch (PA).

On the other hand, if from the material (SL) having a fluidity in which the substance is absorbed into the patch (PA), in addition to depending on the concentration difference in the contact, as described above condition, the additional electrical factors or by changing the physical conditions of the it is possible to control the absorption of the patch (PA). Moreover, it will not come into contact nor the material and the absorbent material that is subject captured in the patch (PA) directly, indirectly via an intermediate contact with the absorption of the material may be performed.

Figure 17 to 19 shows that the absorption material from the patch material (SL) (PA) that has a liquid as an example of absorption of the material of the functions of the patch (PA) according to the present application. Referring to Figure 17 to 19, wherein the patch (PA) may absorb some material (SL) having the fluidity. The absorbing said substances, said patch (PA) is the fluid introduced in the material (SL) with or by contact with the material (SL) with the fluid material in the trapped liquid in the patch (PA) (SB) and this material (SL) having the fluidity can be achieved by being movably to each other.

Here, the patch (PA) has assumed the case of absorbing foreign matter from the other patch (PA).

The patch (PA) that is to absorb the foreign materials from the other patch (PA), absorbed by the outer material and the patch (PA) material and the outer material and the patch (PA) in which the absorption capture group in which the absorption , it can be achieved by a difference in bonding strength between the non-foreign object. For example, the absorbent material which is tinged with hydrophilic attraction between the patch (PA) is material in which the absorption ttimyeo hydrophilicity and the patch is a different patch history of (PA) (PA) and said absorbent material strong when compared to (i.e., the patch (for PA) has a strong hydrophilic nature than that of the other patch (PA)), when separated after the patch (PA) and the other patch (PA) is in contact the the outer material may be at least in part absorbed by the patch (PA).

Figure 20 to 22 shows that the absorption material from the one example of the absorption of the material of the functions of the patch (PA) according to the present application, the patch (PA3) other patch (PA4). Referring to Figure 20 to 22, wherein the patch (PA3) may absorb some of the material that was located in the other patch (PA4). The absorbing said substances, said patch (PA3) other patch (PA4) by contacting the material of the entrapped liquid to said patch (PA3) (SB) and the material of the trapped liquid in the other patch (PA4) (SB ) it may be made by being able to communicate with each other.

On the other hand, according to the patch (PA) a percentage of the total volume of the frame structure of the three-dimensional network structure (NS) constituting the patch (PA), to the binding force of the foreign matter in which the absorption of the patch (PA) change can. For example, the amount of material to be trapped in the structure can be reduced as the the frame structure, the patch (PA) has a volume percentage of the total increase. In this case it can be a bonding strength between the said patch (PA) and the target material decrease for example, to the contact area between the material and the target material captured on the patch (PA) decreases.

In this connection, it is possible to adjust the ratio of the material of the net structure (NS) in the production step of the patch (PA) to control the polarity of the patch (PA). For example, in the case of the patch (PA) produced by using the agarose, by controlling the concentration of the agarose, it is possible to adjust the degree of absorption.

When the extra area in which the patch with respect to the material supplied from (PA) has a weak binding force as compared to the patch (PA), said patch (PA) and the other patch (PA) has been in contact are separated, the absorption that foreign particles may be separated from the other patch (PA) with the patch (PA).

2.2.4.3 the provision of environmental

Patches (PA) according to the present application can be by the above-described characteristic, and perform control of the environmental conditions of the target region to function. The patch (PA) may provide resulting from the patch (PA) in the target region to the environment.

Environmental conditions resulting from the patch (PA), can be dependent on the patch (PA) material (SB) of the trapped liquid in. The patch (PA) can, creating an environment of interest in the material located in the outer region so that, corresponding to the properties of the material contained in the patch (PA) or said patch (PA) from the characteristics of the material which is received.

It is to control the environment, it can be understood by changing the environmental conditions of the target region. Changing the environmental conditions of the target region, a region in which the object to the environment of the patch (PA) form that extends the area on the effect of the to include at least a portion of the target region or the patch (PA) and It may be implemented in the form of shares.

Hereinafter, for convenience referred to as the functionality of the patch (PA) as described above, it provides the environment.

Providing the environment of the patch (PA), it can be carried out in a possible movement of the outer region and a material state in which the patch (PA) is to provide the environment. Provision of the environment by said patch (PA) can be carried out due to the contact. For example, the patch (PA) is the target region of contact with (e.g., foreign object, the plate (PL), and so on), by the patch (PA) can provide a specific environment in an area in which the object .

The patch (PA) is to provide the appropriate pH, osmotic pressure, humidity, density, and temperature of the environment, it is possible to control the environment of the target area (TA). For example, the patch (PA) may grant liquidity (liquidity) onto a target area (TA) or the target material. Grant of such fluid may cause some movement of the entrapped material to the patch (PA). The material of the trapped liquid in the patch (PA) (SB) can be provided to the target area to wetted (wetting / moist) to (TA) environment through the base material (BS).

Environmental factors which are provided by the patch (PA) may be kept constant in accordance with the object. For example, the patch (PA) is capable of providing homeostasis in the area for the purpose. As another example, there is provided a result, environmental conditions of the area in which the object of the environment can be adapted to the material trapped in the patch (PA).

Providing the environment by said patch (PA) may be a result of the diffusion material (SB) of the liquid contained in the patch (PA). That is, if the area in which the object and the patch (PA) in contact, the movement of the material may be possible through the contact area formed due to the contact. In this connection, the setting change by the osmotic pressure, environmental changes in ion concentrations, changes in the PH and provide a moist environment, such as may be implemented in accordance with the diffusion direction of the substances.

Figure 23 to 25 are shown to provide the desired environment for the patch (PA) outside the plate (PL) as an example of providing the environment of the functions of the patch (PA) according to the present application. Referring to Figure 23 to 25, wherein the patch (PA) may provide the desired environment in the fourth material (SB4) and the fifth material outside the plate (PL) the position (SB5). For example, the patch (PA) may provide a desired environment for forming a sixth substance (SB6) by the reaction of the fourth material (SB4) and said fifth material (SB5) to the plate (PL) . Delivery of the environment, wherein the patch (PA) the plate (PL) and the fourth material by contact with the water film (WF) near the contact region is formed in the formed water film (WF) (SB4) and the fifth material may be formed by being (SB5) is to be captured.

3. Apply the patch

Patches (PA) according to the present application, it may be implemented to perform a variety of functions by appropriately applying the function of the above-mentioned patch (PA).

By initiating some embodiments Hereinafter, a description will be made on technical ideas of the present application. However, the technical scope is to be applied or the application functions of the patch (PA) is initiated by the present application will be understood of one of ordinary skill in the art readily expanded in the derived range, the present application is limited by the embodiments described in the specification be the scope of the interpretation will not.

3.1 In-patch

The patch (PA) may provide a reaction zone of the material. In other words, at least a portion of the area affected by the patch (PA) on the area can result in reaction of the material. At this time, the reaction of the materials, the reaction between the material provided from the outside of the material of the liquid that is trapped in the patch (PA) (SB) between, and / or materials of the liquid that is trapped (SB) and said patch (PA) one can. Providing a reaction zone of the material, it may be to enable to promote the reaction of materials.

Here, the term substance (SB) of the liquid that is trapped in the patch (PA), the said patch is input after making a material, wherein the patch (PA) inputted at the time of production of (PA) the patch (PA) is stored materials and which can be temporarily includes at least one of the material trapped in the patch (PA). In other words, if at the time the reaction in the patch (PA) active substances that are trapped in the patch (PA), in any form, and is regardless of whether or not the trapped in the patch (PA), in the patch (PA) It can react. Further, it is also possible the material to be added after the production of the patch (PA) which act as reaction initiation.

The provision of a reaction zone of the reaction is related to the liquid material (SB) which are trapped in the patch (PA) may be, a 2.1.3 (i. E., Provide a reaction space) performed yejeok subset of contents described above. Or, it may be a table of contents 2.1.3 and 2.2.4.2 (i.e., absorption) multi concept of performing the combined functions of the contents described above. The present invention is not limited to this, but may be embodied in the form of two or more functions to merge.

3.1.1 First Embodiment

Hereinafter, the above-mentioned (hereinafter referred to as a service feature) provides functions of absorption and reaction chamber of the patch (PA) will be described on the assumption that the carried out by the single patch (PA). At this time, the absorbing function and the service function may be a function that is performed at the same time, may be a function that is performed in a separate point in time with each other, it is possible to perform a further function is performed sequentially to each other. On the other hand, the patch (PA) is also provided, and said absorbing function as well as further comprising an additional different functions can be seen to be included in this embodiment.

The patch (PA), as described above, it is possible to perform the function of capturing the material, the material can have fluidity, even if the captured. If distribution is uneven and the heterogeneous component of some components of the substance (SB) of the liquid can be spread. Substance (SB) of the liquid even when they are uniformly distributed components of the substance (SB) of the liquid may be a state in which a certain level by the irregular movement of the particle mobility. In this instance, the patch (PA) inside the reaction between a material, such as may occur, such as between a specific binding substance.

For example, the patch (PA) In, addition reaction between which is captured material, the material that has been trapped in the material and the patch (PA) in the newly captured fluid to the patch (PA) for a specific binding to each other it may be possible reactions of the form.

The reaction between the substance and the trapping substance that is in which the fluidity can be performed separately from the arbitrary area that is provided with a material in which the fluidity. For example, the patch (PA) is after absorbing substance with the liquid from any space, the patch (PA) is separated from said arbitrary area, the absorbent material and the patch (PA) the reaction was trapped materials may be produced in the patch (PA).

In addition, the patch (PA) is by carrying out the absorption function for which fluid material can be a reaction to the material that is trapped up. In other words, the response of the material that has been trapped in the patch (PA) of the liquid to the absorption of the substance to trigger the absorption in the material and the patch (PA) may take place. The reaction can be performed in the inner space defined by the patch (PA).

Further, the composition of the material of the liquid (SB) trapped in the patch (PA) can be changed due to the reaction occurring in the inside of the patch (PA),. This, especially when the material that is captured within the patch (PA), reaction before and after the chemical composition and are subject to change. Or it may be a composition distribution changes according to the position in the patch (PA) of the material. This may be due to diffusion or may be illustrated to be due to the particles having a specific force against the other material.

The patch (PA) when due to the reaction changes the composition of the material (SB) of the liquid, the patch (PA) and the patch (PA) material of the external (in the case that the contact material, that the contact material) by the density difference between the part or material absorbed into the patch (PA), is to the outside of the material from the patch (PA) can be discharged the material.

3.1.2 Second Embodiment

Hereinafter, a description will be given of an embodiment the capability to provide a reaction space of the storage function and the material of the patch (PA) to be performed with at least a predetermined time for example. More specifically, it is possible to perform the function of at least a portion of the material (SB) of the liquid stored in the patch (PA) is provided a space for the reaction.

The patch (PA) is capable of storing the material, it is possible to provide a reaction space of the stored material. At this time, the reaction chamber is provided by said patch (PA) can be a surface area of ​​the patch (PA) the net structure the micro-cavity naejineun the patch (PA) to (NS) is formed. In particular, in the case of the coating material to the surface of the material and the patch (PA) are stored in the patch (PA) reaction, the reaction space may be a surface area of ​​the patch (PA).

Reaction space provided by said patch (PA), can perform the role to provide a particular environment condition. Patches (PA) is, while the reaction in the liquid phase of the substance (SB) positioned at said patch (PA) proceeds, it is possible to control the environmental conditions of the reaction. For example, the patch (PA) is capable of performing the function of buffer solution.

The patch (PA) is not in, by storing the substance through a network structure, requires a separate storage container. Further, when the reaction area of ​​the patch (PA) of the surface of the patch (PA), can be easily observed through the surface of the patch (PA). To this end, the shape of the patch (PA) may be modified to design a form is easy to observe.

Substance (SB) of the liquid stored in the patch (PA) is modified or can be reacted with other types of material. Substance (SB) of the liquid stored in the patch (PA), the composition may be a change with the passage of time.

On the other hand, the reaction, or chemical reaction in which the formula is changed, it is possible to sense a physical change of state or a biological response. At this time, the material (SB) of the liquid stored in the patch (PA) may be a mixture comprising a plurality of components or a single component material.

Provided 3.2 breadcrumb (channeling)

Hereinafter, description will be made in the patch (PA), which functions to provide a movement path of the material. More specifically, the patch (PA) is capable of capturing such materials with a fluid, as described above, can absorb, can can be released, and / or save. Described above as a function to each combination of patches (PA), it can implement the various embodiments of the patch (PA), which functions to provide a movement path of the material. However, as more and to initiate some embodiments for the concrete understanding.

3.2.1 Third Embodiment

The patch (PA), the above-mentioned patch (PA) 2.2.4.1 (that is, the table of contents for the transmission) of the function of 2.2.4.2 and may be implemented to perform (i.e., a table of contents for absorption). At this time, the absorbing function and the transfer function may be provided in addition, it may be provided in sequence.

The patch (PA) has to do with the absorption and the transmission function, it is possible to provide a movement path of the material. In particular, it is possible to provide a moving path of the foreign matter by conveying to absorb foreign materials in the outer region.

The said patch (PA) is provided outside the movement path of the material, it may be performed by absorption and release of the foreign object to the foreign object. In more detail, the patch (PA) may absorb the external materials in contact with the foreign matter and passing the foreign material in the outer region in contact with the outer region. At this time, the patch is to (PA) is capturing the foreign materials and passes into the outer region can be carried out in the process similar to the above-described absorption and transmission.

Foreign substance to be absorbed and delivered to the patch (PA) may be liquid phase or solid sangil.

This, through the patch (PA) may be such that the part of the mass transfer to the other foreign matter from the exterior material. The patch (PA) and the foreign matter and other foreign material may be contacted simultaneously. The patch (PA) and the outer material and other foreign matter can be brought into contact with each other, wherein the patch (PA) for different points in time.

Wherein the patch (PA) and the outer material and other foreign materials can be contacted at different points in time. When said each external materials in contact at different points in time, the patches (PA) and the outer material is contacted first, since the outer material and the patch (PA) separated, said patch (PA) and the other external this material may be in contact. At this time, the patches (PA) may be, and temporarily stores the captured material from the outer material.

The patch (PA) may provide a delay of time in addition, while providing the movement route of the material. In addition, the patch (PA) may perform the function of properly controlling the delivery amount and the delivery rate of the material to other foreign material.

On the other hand, such a series of processes is, on the basis of the patch (PA) can be carried out in one direction. As a specific example, the patch is absorbed in the material is made through one side of the (PA), the patch may provide an environment in the inner space of the (PA), material is emitted through the other side facing the one side It can be.

3.2.2 Fourth Embodiment

The patch (PA) It is possible to provide a reaction space above a patch and at the same time absorbing and emitting materials in the functions of (PA) material. The provision of this time, the absorption of the material, release, and the reaction space may be carried out simultaneously or sequentially.

According to one embodiment, the patch (PA) is, in performing the process of absorption and emission of the external substance, the said substance absorbing the outside can provide a reaction space at least for some time. The patch (PA) may provide a specific environment for at least some time in the patch (PA) of the liquid substance (SB) to capture foreign particles, including the said absorbent.

The foreign matter trapped in the patch material liquid that has been trapped in (PA) (SB) and the patch (PA) may be reacted in the inside of the patch (PA). The foreign material absorbed in the patches (PA) may be affected in the environment provided by the patch (PA). Material discharged from said patch (PA) may contain at least some of the substance produced by the reaction. The outer material is changed, such as the composition, characteristics from the patch (PA) can be released.

The absorbent material may be released from the patch (PA). It is the foreign material absorbed in the patches (PA) is released from the patch (PA) may be understood to pass through the patch (PA). The passing through the patch (PA) outside material can lose their identity due to the environment provided by the reaction to the patch (PA) at the inside of the patch (PA).

Delivery of reaction material and the absorbent, the material of the aforementioned outer material can proceed in one direction. In other words, the location of the patch in one (PA) of the absorption material is carried out, and in another position is provided in the environment is performed, it may also be in another position the release of the carried material.

Figure 26 to 28 are shown to provide a movement path of the material between one embodiment of the patch (PA) according to the present application, the two plates (PL). Referring to Figure 26 to 28, wherein the patch (PA) is to provide a movement path of the material between the seventh substance (SB7) a plate (PL1) and the eighth material (SB8) The coated plate (PL2) coating is. As a specific example, the seventh material (SB7) has a binding with the eighth material, the eighth material if it is fixed to the plate (PL2), said patch (PA) the plate (PL1, PL2) by contact with the can of the seventh material (SB7) it is combined with the eighth material (SB8) to move through the patch (PA). The water film (WF) of the seventh material (SB7) and the eighth material (SB8) is formed by contact with it connected to the patch (PA), it said patch (PA) are each plate (PL1, PL2) It can of.

Figure 29 and 30 are shown to provide a movement path of the material between one embodiment of the patch (PA) according to the present application, the two patches. Referring to Figure 29 and 30, the patch (PA6) providing the movement route may be in contact with the patch (PA7) receiving forward patch (PA5) and a mobile target material to store the moving subject matter. Patch the mobile target material subject passing the moving target material by contact with the patch (PA7) receiving forward patch (PA5) and a mobile target material to the patch (PA6) providing the movement route store the moving substance (PA7 ) it may be moved to. The material is moved between each patch, it can be made through a water film (WF) formed in the contact region between the neighborhood of each patch.

Figure 31 and 32 are shown to provide a movement path of the material between one embodiment of a patch according to the present application, the two patches. Referring to Figure 29 and 30, the patch (PA9) providing the movement route may be in contact with a ninth material (SB9) patch (PA10) receiving forward patch (PA8) and the material to store. The patch (PA9) providing the movement route can be absorbed the ninth material (SB9) into contact with the patch (PA8) for storing a ninth material (SB9). The absorbent material of claim 9 (SB9) may form the second material 11 reacts with the material 10 (SB10) that was stored in the patch (PA9) providing the movement route. The material of claim 11 (SB11) may be delivered to the patch (PA10) receiving the material delivered from the patches (PA9) providing the movement route. The material is moved between each patch (PA), it can be made through a water film (WF) formed in the contact region between the neighborhood of each patch (PA).

3.3 multi patch

Patches (PA) is not only be used alone, a plurality of patches (PA) may be used together. In this case, also there is a plurality of patches (PA) may be used together, as well as when used at the same time also includes when used sequentially.

If the patch (PA) of the plurality to be used at the same time, each of the patches (PA) may perform different functions. Each patch (PA) of said plurality of patches (PA), but may store the same material, and may store the different materials.

If the patch (PA) of the plurality to be used at the same time, each patch (PA) is to not be in contact with each other move in between the patch (PA) material can not take place, or interaction of the material stored in each patch (PA) it is possible to perform a desired function in the state.

A plurality of patches (PA) to be used together, but can be manufactured to the same specifications naejineun another similar shape, may be used with each other in case of a plurality of patches (PA) having a different shape. Further, each patch (PA) constituting the plurality of patches (PA) is the density of the net structure (NS) or different from each other, that make up the netting structure (NS) component may also be produced differently.

3.3.1 Multi-contact patch

When using a plurality of patches (PA), it is a target area to a plurality of patches (PA) in (TA) to be contacted. The plurality of patches (PA) may perform the function desired in contact with a target area (TA).

In the case where the plurality of patches (PA) is in the target area (TA) with a plurality, and each other may be in contact with the other target area (TA). The plurality of patches (PA) may perform the function desired in contact with the target area (TA) which respectively correspond to the case that the target area (TA) with a plurality.

The plurality of patches (PA) may be in contact with the material which is applied on the target area (TA). At this time, the material applied to the target area (TA) may have a fixed or fluid.

Function of the object, may be delivered to the absorption capabilities of the material. However, be sure that each patch (PA) is transmitted or not is to be absorbed to the same material, each patch (PA) is in or passed to another target different material regions (TA), located in the target area (TA) of the same material It can absorb different components from the material.

Function of the object, each patch (PA) in which the configuration of the plurality of patches (PA) may be different from each other. For example, one patch (PA) performs the function to transfer the material to a target area (TA) and the other patch (PA) it is also possible to perform the function of absorbing a substance from the target area (TA).

The different materials, wherein the plurality of patches (PA) comprises a different material can be used to induce a reaction of interest is transmitted to a target area (TA). In the case where the material of the plurality of components required for the reaction to occur to the objects, and each store a material of the plurality of components in the patch (PA) in the plurality, can be transmitted to the target area (TA). The use of such a plurality of patches (PA) is a substance required for the reaction may be particularly useful in the case where when mixed, such as being stored on a single patch (PA) reasons, the loss of properties of the material required for a desired reaction or deterioration is.

According to one embodiment, the plurality of patches (PA) to each other in the case comprises a material of the other component and having a material of different specific binding relationship between the different components, the materials of the different components, the target area ( It can pass on TA). The plurality of patches (PA), can be used by passing the materials of the different components to detect a plurality of specific binding from the material applied to the target area (TA).

According to other embodiments, but a plurality of patches (PA) comprises a material of the same component with each other, each patch (PA) may have a different concentration with respect to the materials of the same component. Wherein each plurality of patches comprising a material of the same component (PA) can be used to be in contact with the target area (TA) to determine the effect of concentration of a substance contained in the patch (PA) of the plurality.

On the other hand, in the case of using a plurality of patches (PA) as described above, it can be used to transform the bundle of patches (PA) in a more efficient form. In other words, can be used by changing each time to implement the constitution of the plurality of patches (PA), it is used. That is, it can be used to produce a plurality of patches (PA) to the cartridge type. At this time, it may be produced by appropriately normalized in the form of individual patches (PA) is used.

A plurality of patches (PA) of the cartridge type, to produce a patch (PA) for storing a plurality of kinds of materials, respectively, may be suitable if it is desired to use the trade-offs, as needed.

In particular, by using a plurality of types of materials, the target area (TA) can be carried out by the configuration, unlike the combination of the specific reaction to be detected each time that if it is desired to detect a specific behavior of each substance, subjected to detection from There will be.

33 shows that it is used as an embodiment of the patch (PA) according to the present application, with a plurality of patches (PA). Referring to Figure 33, a plurality of patches (PA) according to an embodiment of the present application can be contacted at the same time on the target area (TA) which is located on a plate (PL). Each patch (PA) in which the configuration of the plurality of patches (PA), may have a standardized form. The plurality of patches (PA) is stored in the material and the first patch comprises a first patch and the second patch may be different from the material stored in the second patch.

Figure 34 is a plurality of patches (PA) are used together, it said plate (PL) is shown to include a plurality of target areas (TA). According to FIG 34, a plurality of patches (PA) according to an embodiment of the present application it can be contacted at the same time to a plurality of target areas (TA) which is located on a plate (PL). The plurality of patches (PA) is the first patch (PA) and the second patch (PA) included, and the plurality of the target area (TA) a comprises a first target domain and a second target area, said first wherein the patch is in contact with the first target region and the second patch may be in contact with the second target area.

3.3.2 Fifth Embodiment

The plurality of patches (PA) is capable of performing a plurality of functions. Each patch (PA), each patch (PA), as well as can perform a plurality of functions at the same time that may perform different functions at the same time as described above. However, it is also possible that nor not limited to the above case, each function are combined in a plurality of patches (PA).

First of all, as if the individual patches (PA) to perform a plurality of functions simultaneously, and each patch (PA) that can perform both the storage and release of the substance. For example, each of the patches (PA) that can be stored, and emits each of the stored material in the target area (TA) of different materials. In this case, each of the stored material can be discharged to the same time or sequentially.

Next, each of the patches (PA) is also carried out each other as the case of performing other functions at the same time, each of the patches (PA) is divided into the storage and release of the substance. In this case, only a portion of each patch (PA) can be brought into contact with the target area (TA), and release the substance to the target area (TA).

3.3.3 Sixth Embodiment

When a plurality of patches (PA) is used, a plurality of patches (PA) as described above may be carried out a plurality of functions. First, the individual patches (PA) at the same time to perform the storage, release and absorption of the material at the same time. Alternatively, it is also possible that each patch (PA) is carried out by dividing the storage, release and absorption of the material. However, nor not limited to this, it is also possible that each function are combined in a plurality of patches (PA).

In one embodiment, at least some of the plurality of patches (PA) may store a substance, to release the stored material target area (TA). At this time, the other at least one portion of the plurality of patches (PA) is capable of absorbing a substance from the target area (TA). One or more of the patches (PA) of the plurality can emit a substance that specifically binds to and located at the target area (TA) material. At this time, it will be able to perform the detection of specific binding substances other than those formed by absorbing the specific binding of the substance located in the target area (TA) by using other patch (PA).

3.3.4 Seventh Embodiment

When a plurality of patches (PA) are used, the individual patches (PA) can be carried out at the same time the provision of storage and discharge of the material and the environment at the same time. Alternatively, the individual patches (PA) that can be performed by dividing the service of storage, release, and environment of the material. However, nor not limited to this, it is also possible that each function are combined in a plurality of patches (PA).

For example, one patch (PA) of a plurality of patches (PA) is capable of releasing a stored material to the target area (TA). At this time, the other patch (PA) may provide an environment to the target area (TA). Here, it may be implemented to provide an environment, the environment condition of the material stored in the other patch (PA) in the form of delivery to the target area (TA). More specifically, the one is provided with a reaction material in the target area (TA) by the patch (PA), the other patch (PA) is capable of providing a buffered environment in contact with the target area (TA).

As another example, there may be a plurality of patches (PA) are in contact with each other. At this time, to the other patch (PA) to at least one patch (PA) stores a material, and provides an environment, it is possible to discharge the stored material. In this embodiment, the patch (PA), which provides an environment may emit substances, each in contact with at least one patch (PA) which do not contact each other, the absorbent material from the individual patches (PA).

4. Blood tests (Hematologic diagnosis)

4.1 Significance

Patches of the present application may be used for blood test. It means for checking the blood along with the hematological method to diagnose the blood test is the absence of health or sickness or disease of the inspectors, progress, etc.

Patches of the present application may be used in a variety of blood test that water droplets, obtaining morphological information about the blood. Hereinafter, reference may be made to the representative few blood tests using the patch of the present application. However, it puts out a not limited in this application to the example in which the blood test will be described later.

Further, in the application of the patch of the present application to a blood test, it can be changed above a base material (BS) and the additive material (AS) is properly in accordance with the Application The.

Representative examples of the blood test 4.2

4.2.1 General blood test (CBC: Complete Blood cell Count)

Typical example of the blood test, the CBC: may be mentioned (CBC Complete Blood Count cell). CBC is one of blood, i.e., red blood cells, white blood cells, and the basic blood test using the numerical information and morphological (morphology) information about the platelets having a variety of clinical indications, from the observation diagnosis, treatment and tracking of disease present in the blood .

4.2.2 peripheral blood smear (peripheral blood smear examination)

Representative examples of different blood tests, there can be mentioned a peripheral blood smear. Peripheral blood smear after smear is a blood test that blood on a glass slide staining to examine the numerical or morphological information of the blood cells through a microscope or bacteria or parasites found in the blood. For example, in the case of red blood cells may be differentiated classification and cause of the anemia, it helps to determine the case of the white blood cells, myelodysplastic syndrome, leukemia, cause infection or inflammation, giant cell anemia enemy whether the like. Also it platelets is to help differentiate such as myeloproliferative disease, platelet satellites phenomenon. In addition, it is possible to detect a variety of bacteria, parasites and other pathogens, such as malaria or tuberculosis present in the blood.

Examples of staining 4.3

Blood tests in the present application can be primarily performed by the smear of blood on a plate (PL), such as a slide glass and observed after the dyed blood staining them. Here, a variety of staining may be used, if necessary. For example, staining of the blood compartments include gimja staining (Giemsa staining) and Wright stain (Wright staining), Wright gimja staining (Giemsa-Wright staining) (Romanowsky staining) Romanov ski staining, such may be used. In addition to the staining method, such as simple staining (simple staining) or Gram staining (Gram staining), AFB staining (Ziehl-Neelsen stainig) or to investigate the cervical Papanicolaou mainly used (Papanicolaou staining) involved in bacteria test this application a blood test can be used.

Blood tests carried out in 4.4

4.4.1 Preparation of the sample

Here, a description will be given with respect to the sample used for the blood test, that is the preparation of blood.

Preparation of a specimen may be located on a plate (PL) in order to check the blood using a patch (PA) of the present application.

Plate (PL) herein may represent a solid plate such as a plate made of, a general slide glass, polystyrene (polystyrene) or polypropylene (polypropyrene) and the like. In addition, the plate (PL) is, may be in the form of a transparency or other floor it can be used in accordance with the detection method. It said plate (PL) may comprise a reaction zone that can lead to the reaction in contact with the object or the patch (PA).

4.4.2 Preparation of Patch

In carrying out blood tests in the present application, the above-mentioned patch (PA) may be used.

The patch (PA), may pass storing dyed sample, and to the plate (PL). Here, the sample was dyed may be varied depending on the stain to be performed for the purpose of blood or them. Representative examples of the dye sample is ethyl carmine, Mel ethylene blue, eosin, acid huksin, four plastic Nin, Janus Green B, H. motok published, Kim jaaek, light amount, light-Romano ski staining (Romanowsky stain), such as steaming jaaek staining solution to be used, there may be a Li syumeon such as dye, gram staining solution, carboxylic ball soft, GL (Ziehl) solution. Of course, not limited by the sample dyed by the above described in the present invention, illustrated, in addition there is a wide range of substances that stain the blood as needed, it can be used to dye the sample.

Here, the patch (PA), it is possible that the staining of the sample single-only storage. For example, if it is desired to perform a staining method using only one dye sample as simple staining patch (PA) is methylene blue (Methylene blue) to be used in a simple dye, Crystal Violet (Crystal violet), four plastic Nin (Safranin ) you can save only one kind of dye, such as a sample.

In addition, where the patch (PA), it is possible that more than one dye samples are stored together. For example, the patch (PA) if it is desired to perform a staining method using together two or more stained sample as gimja dye may store the two dye sample, such as methylene blue to jayeom the eosin and nucleus of jeokyeom the cytoplasm at the same time .

Of course, even in the case to perform a staining method using a sample with more than one dye, it does not need to store all dyed samples that must be used for staining, patches (PA), as described above. That is, the patch (PA) may store a subset of the plurality of staining the sample is used for staining. For example, it is also possible to use as the patch (PA) for storing only a single dye sample patches (PA) and methylene blue or very word store only a single specimen of a blue dye and is the eosin perform gimja.

Further patch (PA) may comprise a material that the discoloration and mordanting materials in addition to staining the cells directly as a dye sample. For example, to perform the Gram staining method, the present dye is Crystal Violet (Crystal Violet) to store the patch (PA), a patch to store the four plastic Nin (Safranin) contrast dye (PA), which, mordant of iodine patch to store the patch alcohol (PA), and bleach to store (Iodine) may be (PA) is prepared.

Further patch (PA) is capable of storing a fixed (fixation) solution, and fixing the blood smeared on the plate (PL). Fixing solution as mainly the like alcohol, formaldehyde, such as ethanol or methanol may be used.

If the patch material (PA) are stored hydrophobic patch (PA) can also be prepared so as to have hydrophobicity. For example, it is true in the case of the patch (PA) including the fixing solution or bleach alcohols. As a material for the hydrophobic patches (PA) has a gel such as PDMS or PMMA gel, silicon gel.

Or patch (PA) for storing the fixed agent and bleach may be replaced by a solid material that solidified the fixing agent and bleach. Such examples include the solidified methanol (solidified-methanol).

Further patch (PA) may save the washing solution, and absorption of the residue from the plate (PL). The patch (PA) may be removed by absorbing the impurities of the plate (PL) by being stored the wash solution and the separation was brought into contact with the patch (PA). Wash solution in which the use can have a TBS or PBS with the addition of tween-20.

Further patch (PA) may store a buffer solution, and providing an environment to the plate (PL). In this case, a buffer solution, may play a role in each phase of the blood to be smoothly performed. Thus, the buffer solution to be used at each stage may comprise other components. Buffer solution is a solution having the indicated pH-specific staining can be used.

Patches (PA) described above may be used individually or used in combination. For example, the patch (PA) is capable of storing with a dyed sample and the buffer solution.

It is described in more detail below with respect to the performance of a blood test using the patch (PA)

4.4.3 Method of blood

Here, a description is made of some typical examples of the method using a patch (PA) and the plate (PL) in the present application as described above, to perform a blood test.

However, the blood test of the present invention method is not limited to the below example, the modified detection method is in accordance with that plurality may be present, to be applied across the blood test method overall is performed using the patch (PA) It will be.

You may use the patch (PA) and the plate (PL) of the present application can perform a blood test.

Using the patch (PA) and the plate (PL) blood tests, and smear the sample located in the plate (PL), and by contact of the patch (PA) in a smear sample stained sample, by detecting the staining results It can be carried out.

4.4.3.1 smear of blood

The smear of blood on a plate (PL) can be carried out by a variety of methods. Here, the blood smear may be plated with a single layer or multi-layer as required.

Figure 35 (new) and 36 (new) is a diagram according to an embodiment of the blood smear method according to an embodiment of the invention.

According to one embodiment, it is to smear the sample on a plate (PL) using a smear member (other slide glass or plated film, and so on) for the rear dropped blood droplets on the plate (PL) the blood to the plate (PL) smear can be.

Referring to Figure 35, it is possible to slide in the one direction by passing through the blood smear the drop of the drop member (SM) for on a slide glass so that the blood is smeared on a slide glass. At this time, while the blood is pushed to the physical by plated members may be unfolded on a slide glass.

Or it may be 36, by sliding a drop of blood drop smeared member (SM) for on a slide glass to which the sliding in one direction up to the contact points back in the opposite direction so that the blood is smeared on a slide glass. At this time, by capillary action to occur between the blood smeared slide glass member and for which follows the plated member in the opposite direction can be unfolded on a slide glass.

37 is a view of another example of the blood smear method according to an embodiment of the invention.

According to another example, to smear the sample on a plate (PL) is a smear for the nozzle for ejecting the blood as a single layer to smear the blood on the plate (PL) by moving the plate (PL) on. Here, the smear nozzle may jet the blood injected by using a micro channel to a single layer.

By Referring to Figure 37, the injection of blood moving along a predetermined path smear nozzle (SN) for this on the slide glass may be such that the blood is smeared on a slide glass.

4.4.3.2 Blood stained the

Staining of blood can be carried out by contacting the patch (PA) containing the dye sample in a blood smear on a plate (PL). After the blood smear and patches (PA) are in contact, and the granules are dyed sample in the patch (PA) to move to the blood existing in the interior, such as the cytoplasm or nucleus, or white blood cells in the blood it can be dyed.

Patches (PA) containing nuclear staining samples may be stained with the nucleus of the blood. By nuclear staining samples it is generally used a basic dyed sample, and typical examples are such as methylene blue (methylene blue), toluidine blue (toluidine blue), hematoxylin (hymatoxylin). Basic dyed sample (-), it is charged as a patch (PA) that can be dyed if it coupled to the nucleus and which is charged to the patch in the blood to move the blood from (PA) (+), in contact with blood.

Patch containing the cytoplasmic staining the sample (PA) is capable of dyeing the cytoplasm or cell outer structure of the blood. With cytoplasmic staining the sample is used mainly acidic dye sample, and the like that is a typical example eosin (eosin), huksin acid (acid fuchsin), Orange G (oreng G). Psoriatic stained samples has become charged with (+) patch (PA) is in contact with a blood patch in the blood to move the blood from (PA) (-) in combination with the cytoplasm or cell outside the region that is charged to be dyed this can.

In the case of granules, it can be stained with an appropriate color by the basic dye-dyed sample and the acidic sample. For example, granules may be stained with methylene blue and violet by Shin Io.

Of course, not using the dye sample patches (PA), which is necessarily above the blood stain.

For example, the patch (PA) is a part and charged with (+) - can store the charged area with at the same time neutral dye samples (neutral stain) equipped with (). If the patch (PA) for storing a mild staining the sample is brought into contact with blood, some of the dye sample that - the region is (e.g., cellular) in a (+) region of blood in combination with a dyeing it with a predetermined color () another part is the (+) in the area of ​​blood-may be coupled to dye it with the predetermined color and a different color and region (e.g., nucleotides) (). Typical examples of neutral dye samples may be stained a light sample.

On the other hand, in the description of the above dye it has been described about the color development of dye dyeing the actual object, but may be alternatively a fluorescent substance for fluorescent staining object instead of staining the sample. For example, to observe the nucleus will be able to fluorescent nuclear through the method of adding the fluorescent substance to the substance binding to the nucleus.

4.4.3.3 Blood tests

Blood tests may be performed imaging the image of the stained blood, and by analyzing the captured image.

Here, the image pickup of the image may be performed using an optical device. Optics but may have any detecting a blood cell or pathogen, such as bacteria in the blood, such as the red blood cells or white blood cells or platelets in the blood stained if devices capable of obtaining an image of a blood stained with appropriate scaling would. For example, the optical apparatus may be provided with a memory, which stores the captured image from the lens, a CCD or CMOS for controlling the lens barrel, the magnification and focal length provided by an optical sensor, an optical path that is constituted by a CCD or CMOS .

38 and 39 are views directed to imaging the stained blood in the embodiment;

When Figs. 38 and see Fig. 39, the optical device (OD) may be picked up directly from the plated state of the stained blood using the patch (PA) to the plates (PL). Here, the optical device (OD) may obtain the images of the stained blood receiving incident light transmitted to the light source (LS) is irradiated with the stained blood smears plate (PL) it is from.

For example, referring to Figure 38, (OD), an optical device side of the blood smear surface of the slide glass is placed on a (hereinafter referred to as 'front') a light source (LS) is opposite to the front surface of the slide glass, that is, the back surface on may be disposed. According to this arrangement the optical device (OD) is irradiated from the light source (LS) on the back surface side of the slide glass receiving incident light passing through the slide glass can acquire an image of the stained blood.

In other examples, with reference to Figure 39, an optical device (OD) is disposed on the back surface of the slide glass, the light source (LS) can be arranged on the front surface of the slide glass. Optical equipment according to this arrangement (OD) may obtain an image of the blood stain on the front side is irradiated from the light source (LS) receives the incident light passing through the slide glass, the slide glass.

Here, the plate (PL) may be desirable for the light that is emitted from the light source can be prepared from a material that is well permeable. Also the light source may be a white light or outgoing light gwanghal output a wavelength of a predetermined band.

However, when you need to make a high magnification, it may be desirable to be observed without having to pass through the plate (PL) or patch (PA).

Blood tests may be performed by obtaining a variety of information from the captured image.

For example, the image may be provided to the examiner, etc. through the monitor of the computer and medical equipment. The tester can determine from the image and presence of the number and types of blood cells or bacteria presence, number or shape, etc., etc. to determine the health or disease state of the patient based on it.

As another example, it is possible to acquire images from the electronic device, an optical device with image analysis program is installed and generating information about the presence or absence, number and shape of the blood cell numbers and types of bacteria or from an image. The resulting information can be provided to the tester via a monitor of a computer or medical equipment. Based on the information provided Inspectors may determine, such as health or disease state of the patient.

As another example, there is provided an information image analysis program that generates an electronic device installed in an electronic device is a blood test programs installed, based on the information, the electronic device is a blood test programs installed that came to judge, such as health or disease state of the patient it is possible. Here, a single electronic device is an image analysis program and a blood test program installed together can also be performed with the image analysis operation and blood test operation.

Image analysis programs can analyze the captured images. Specifically, the image analysis program is able to obtain numerical information and morphological information of the blood cells and bacteria in the blood from the captured image.

Image analysis program may determine the type of the blood cell from the captured image. Types of blood cells including white blood cells and the red blood cells, or platelets, may contain even more detailed types of white blood cells. Another image analysis program can determine whether the abnormality of blood cells. Here, the image analysis program may determine whether more than the blood cell type or on the basis of size and shape of blood cells within the image.

Another image analysis program can determine the presence of bacteria in the blood.

Another image analysis program can count the number of each type of blood cell count, the number of blood cells and bacteria over.

Blood test program numerical information regarding the blood cells and bacteria (e.g., blood cells by number, more than the number of blood cells, the number of bacteria, etc.) and the morphological information (the blood cells form or in the form of bacteria, and so on) the patient's health based on It can determine the progression of the condition or disease, such as whether or disease.

At least one of the above-described image analysis program and a blood test program may perform a judgment process, according to the algorithm described above is formed through machine learning, such as learning or deep (deep learning) according to a predetermined algorithm.

4.5 Blood Test Example

Figure 40 shows a flow chart for explaining an example of the blood test method according to the present application.

Blood test method according to an embodiment of the present application is the in the reaction zone by a step (S200), and patches (PA) to store the dye sample to stain the blood to position the blood is the test object to the reaction region It may include a step (S300) for delivering dye sample.

Step (S200) of placing the blood on which the test object, can be performed by the above-mentioned sample A method for the smear to the plate (PL).

Figure 41 shows a flow chart for explaining an example of the step of transferring the dye sample to the reaction zone of the methods blood test in accordance with one embodiment of the present application.

Referring to Figure 41, step (S300) for transferring the dye sample to the reaction zone, the step (S310) the dyed sample is brought into contact in the reaction zone of said patch (PA) so as to be movable in the reaction zone, and It may include a step (S320) of separating the patches (PA) from the reaction zone.

If the patch (PA) to store the dye is in contact with the blood sample are stained sample in (S310), the patch (PA) moving the reaction zone, and it is possible to dye the blood. For example, if the patch (PA) stores a basic dye in contact with the blood sample, the sample is a basic dye can dye the nucleus or the nucleus in the presence of bacteria in blood of white blood cells moving in the blood to the reaction zone. For another example, if a store acidic dye sample patches (PA) is in contact with the blood, the samples are acidic dye can stain the cytoplasm and cell outer structure of the moving blood cells and bacteria into the reaction zone.

Remove the patches (PA) from the reaction zone (S320). If you have time to keep in contact too short, difficult to dye it is sufficiently achieved. Conversely, to maintain the contact time is too long, it has been moved, as well as the time of the whole blood test is increased dye sample over into the blood and the quality of the dye can be lowered. Thus, after the patch (PA) is moved to the reaction zone a suitable time to remove the patch (PA) from the contact area. Maintaining the contact time may be suitably designed in consideration of the ambient conditions, such as Density of the dye concentration of the sample, the gel structure of the patch (PA), the temperature conditions.

Figure 42 shows a flow chart for explaining another example of the step of transferring the dye sample to the reaction zone of the methods blood test in accordance with one embodiment of the present application.

Referring to Figure 42, step (S300) for transferring the dye sample to the reaction zone, the method comprising: 1 dyed sample is contacting the first patch (PA) to the reaction zone so as to be movable in the reaction zone (S330 ), the first step (S340), the step of second stain the sample is contacted to the second patch (PA) to the reaction zone so as to be movable in the reaction zone (S350) to the patch (PA) separated from the reaction zone It may include a step (S360) of separating the first patch (PA) from the reaction zone.

Here, the first patch (PA) and the second patch (PA) is a patch (PA) for storing the first staining the sample with the second dye to dye the sample for the other components of the blood, respectively. For example, the first staining the sample is any one of the basic dye and the samples acid sample, the second sample dyeing may be the other of the basic dyed sample and the acid dye sample. In this way, the first patch (PA) and the second dye patch one of (PA) is dyed the cytoplasm or cell outer structure of the cytoplasm and cell outside of my blood cell blood structure, bacteria, and the other is the cell nucleus of the blood cells in the blood or It can stain the nucleus of bacteria

If it is necessary to use three or more dye sample to blood staining (e.g., the dye, the control dye, a mordant, etc.), adding a dye patch as required number (PA) and adds the stained reaction by patch (PA) it is also possible to perform the separating and contacting to the zone.

Figure 43 shows a flow chart for explaining another example of the blood test method according to the present application.

Referring to Figure 43, the blood test method, in addition to the step (S300) for transferring the dye sample in steps (S200) and the reaction zone, positioning the sample, said reaction zone using the wash patch (PA) from may further include a step (S600) for absorbing the foreign matter. Here, the wash patch (PA) may be a patch (PA) for storing a washing solution.

Figure 44 shows a flow chart for explaining an example of the step of removing foreign material from the reaction zone of the blood test method according to another embodiment of the present application.

Referring to Figure 44, step (S600) for absorbing the foreign matter from the reaction zone using the wash patch (PA), the said patch (PA) to be a foreign matter moving in wash patch (PA) from the reaction zone It may include a step (S620) of separating the step (S610) and the patch (PA) is contacted in the reaction zone from the reaction zone.

Patches (PA) for storing a washing solution that is on contact with blood to foreign substances remaining in the reaction zone of (S610), dyeing process, can be absorbed into the patch (PA). When after washing to remove the patch (PA) from the reaction zone (S620), the patch may be (PA) has to be absorbing a water film formed between the plate (PL) and the patch (PA) to absorb foreign materials in the water film (WF).

Figure 45 shows a flow chart for explaining another example of the blood test method according to the present application.

Referring to Figure 45, the blood test method, in addition to the step (S300) for transferring the dye sample in steps (S200) and the reaction zone for positioning the specimen, using a buffer patch (PA) wherein the reaction area in may further comprise a step (S800) to provide the desired environment. Here, the buffer may be a patch (PA) is a patch (PA) for storing a buffer solution.

Figure 46 shows a flow chart for explaining an example of providing a predetermined reaction environment in the area of ​​the blood test method according to another embodiment of the present application.

Referring to Figure 46, using a buffer patch (PA) stage (S800) to provide the desired environment in the reaction region, the predetermined setting the buffer patch (PA) to the reaction zone in contact with the reaction zone a step (S810), and the buffer patch (PA) providing may include the step (S820) of separating from the reaction zone.

If the patch (PA) for storing a buffer solution is in contact with the reaction zone (S810), in the reaction zone, the stained sample can be a predetermined condition required to dye the blood formation. For example, if the buffer patch (PA) is in contact with the reaction zone, the pH of the water film (WF) between the buffer patch (PA) and the plate (PL) can be a point pH for staining by a buffer solution, and thus and the quality of dye can be improved. When the from the dye patch (PA) moves to the dye sample over the blood of the blood cells and bacteria such as perchloric color or to use a plurality of staining the sample with a first dye patch (PA) and the second dye patch (PA) If the blood is stained with two or more stained sample buffer patch is (PA) that can improve the quality of the dyeing by creating an appropriate environment for the dye in the reaction zone

Buffer Separating the patch (PA) from the reaction zone (S820), the water film (WF) with this buffer patch (PA) staining the sample remaining dyed sample is water film (WF) are not combined with the blood of as absorption into the buffer patch (PA ) it can be absorbed in. Thus in some cases, the excessive blood is stained sample injected, on contact, to remove the buffer patch (PA) to the reaction region can be prevented from being degraded, the quality of dyeing.

Figure 47 shows a flow chart for explaining still another example of re-blood test method according to the present application.

Referring to Figure 47, perform a blood test method of the present application may further include a step (S100) and a step of fixing a blood smear (S120) to smear the blood on the plate (PL).

To applying the blood to the plate (PL) (S100), it can be to smear the blood as a single-layer (monolayer) to thin film equivalent.

When performing a diagnosis to smear the blood so as to conform to a single layer, as described above, the effective surface area of ​​the patch (PA) in contact with the blood and the plate (PL) spread on the plates (PL) can be maximized. In other words, by the blood smear and to perform target detection contact patches (PA), it has the effect that a valid result is also obtained with a small amount of blood. In order to enlarge the effective surface area it is to complicate the design of the distribution area of ​​the blood can be a very simple implementation of the reaction zone as compared to the conventional blood test method who perform diagnosis.

In addition, when the blood is smeared into a thin film, there is a possible advantage Quantitative analysis if the disease progress test or normal blood test (CBC) through the image analysis.

Of course, if necessary, it is also possible to smear a film-like (thick layer) having a constant thickness instead of a thin film (thin layer).

To (S120) for fixing the blood to the plate (PL), it can be fixed to smear the blood as a single-layer (monolayer) to thin film equivalent.

By using the above-described washing patch (PA) using the step (S600) and buffers patch (PA) for absorbing the foreign matter from the reaction zone step (S800) to provide the desired environment in the reaction zone stores stained sample using the patch (PA) to convey the sample dyed in the blood that may be performed in at least one time of before and after. However, it is to carry out the step S600 or step S800 after the transmission of the stained sample may be desired in order to improve the quality of the final dyeing.

In steps S600 and S800 respectively, with each other when brought into contact with, and separate a plurality of patches (PA) for storing different dyed samples in blood, a plurality of patches (PA) and blood pre-contact, contact and after a plurality of patches of It can be carried out in at least one point of contact between the point in time. However, it is to carry out the step S600 and step S800 after the transmission of the stained sample may be desired in order to improve the quality of the final dyeing.

In addition the buffer may be Patched (PA) it is also possible to carry out the function of washing the patch (PA), step S600 is carried out using a buffer patch (PA). According to this, since the patch buffer (PA) are in contact in the blood to perform the washing function with a buffer function, the buffer can be carried out with the step S800 in the process of step S800 is performed by the patch (PA).

Similarly, it is also possible that also wash patch (PA) it is also possible to carry out the functions of the patch buffer (PA), step S800 is performed using the wash patch (PA). According to this, since the wash patch (PA) are in contact in the blood to perform a buffer function with a washing function it may be performed with the step S600 in the process of step S600 is performed by the wash patch (PA).

Further it may be a patch (PA) to store the dye sample patches to function as a buffer (PA) when the patch (PA) to store dyed sample using a buffer solution as an internal solvent. Accordingly, since dye patch (PA) are in contact in the blood to perform a buffer function with a dye functionality, it can be performed with the step S800 in the process of the step S200 performed by the dye patches (PA).

In may cases the patch (PA) to store dyed sample using a washing solution as a solvent inside the patch (PA) to store the dye sample to serve as wash patch (PA). Accordingly, since dye patch (PA) are in contact in the blood to perform the washing function with a dye functionality, it can be performed with the step S600 in the process of the step S200 performed by the dye patches (PA).

Further it may be a patch (PA) for storing a function to wash stained sample patches (PA) and a buffer patch (PA) when the patch (PA) to store dyed sample using a wash solution, or buffer solution as an internal solvent. Accordingly, dye patch (PA) is in contact with blood dyeing capabilities and with washing function and therefore to perform a buffer function, dye patch (PA) in step S200 a step in the process is carried out S600 and step S800 is to be performed together by can.

Hereinafter, with respect to specific methods for performing blood geomsaeul using the patch (PA) and the plate (PL), it will be described for some embodiments.

4.5.1 Based on Examples 1 - Simple dyeing

Blood tests in accordance with one embodiment of the present application, using a plate (PL) and the patch (PA) can be carried out by simple staining.

Figure 48 shows a flowchart illustrating a blood test method according to the simple staining as an example of a blood test method according to the present application.

Blood by simple staining, according to one embodiment of the present application method is for recording an image according to the step (S300), staining for placing the blood into the reaction area (S200), passes the dye sample to the reaction area a step (S400), and images can be analyzed, including the step (S500) of performing a blood test.

Transferring the dye from the blood sample by simple staining (S300) and delivers the one days stained sample to the blood. In this embodiment, for this purpose it can be usually carried out using a dye patch, one to store a single dye samples.

Step (S300) to pass stained sample before the step (S310), and stained patches (PA) of contacting the dye patch (PA) to store the dye sample to the reaction area on the plate (PL), such as a slide glass plate (PL ) may include the step (S320) of separating from.

49 is a view showing a process of staining the sample is delivered by the blood test method according to the simple staining according to the present application.

Referring to Figure 49, stained patches (PA), it can store the dyed sample, and pass the samples stained with the blood located on the plate (PL) (S310). The said patch (PA) is delivered to the dye sample to the plate (PL), wherein the dyed sample the patch (PA) 20 through a water film (WF) formed in the neighboring contact areas by contact with the plate (PL) being movable in the reaction zone of the plate (PL) or the plate (PL) can be achieved.

The dye may be a sample of the reaction between is delivered to the plate (PL), the dyed sample and the blood, especially in the nucleus, such as blood cells and bacteria contained in the blood, the cytoplasm, the cell outer structure. In other words, the dye patch (PA) are in contact in the reaction zone, dyeing, and the smear, staining the sample with a fixed blood to the reaction zone moves from the patch (PA), a mobile dye samples were stained them in combination with the target substance in the blood can.

Here, if the dye can be made smoothly using the buffer solution as a solvent to the dye patch (PA). Of course, it is also possible to use a buffer in order to implement the patch appropriate for a given environment staining on the reaction zone.

After staining the sample is delivered to the blood sufficiently to separate the dye patch (PA) from the reaction zone (S320). At this time, the target material react with the dye in the blood sample is not a bond, and remain in the reaction zone in a state combined with the target material surplus dye samples can be absorbed back into the stain patch (PA).

Depending on the detail, with blood nor not form a bond, stained sample movement to the plate (PL) is a patch (PA) storing dye sample separated from the plate (PL), it is absorbed by the dye patches (PA) It may be removed from the plate (PL). Here, the excess staining the sample is absorbed into the dye patch (PA), excess dye samples are remaining in the dye patch (PA) a water film (WF) who formed in contact with said plate (PL), and the dye patch (PA ) is the water film (WF) when separated from the plate (PL) is hooked to the dye patch (PA) it can be carried out by the mobile.

There slightly stained sample between in the process is to remain in the not absorbed by dye patch (PA) blood, it is possible to contact and remove the remaining dye sample remaining over not separate from the reaction zone a buffer patch or wash patch.

Of course, depending on the excess stain the sample is removed from the plate (PL) only by the separation of the dye patch (PA), it is also possible to process the wash buffer and the process was essentially required in the yeomsaekreul performed for a conventional blood test to be omitted. In other words, according to this embodiment, a separate washing process for removing the residual stain sample using a wash solution from the plate (PL) can be omitted.

May When dyeing is completed, the analysis of an image obtained by capturing an image of the reaction zone of the plate (PL) obtaining a dye image (S400), performing a blood test (S500).

In step (S120), washing the patch (PA) for fixing the step (S100), the smeared blood is a blood test method according to the simple staining in accordance with one embodiment of the present application, smear the blood on the aforementioned plate (PL) a it is at least one of the steps (S800) to provide the desired environment in the reaction zone by a step (S600), and buffer patch (PA) washing the reaction zone may further include using. Here, step S100 and step S120 may be performed before the time point of step S200. Also here, step S600 and step S800 may be performed at least once at the time between each step S200 and step S400.

Step (S600) of washing the reaction zone using the wash patch (PA), can wash the patch (PA) is intended to absorb the residual water in contact with the plate (PL). The wash is to absorb the residual water using a patch (PA), the wash patch (PA) exists in at least a part of the unreacted stained samples or the reaction zone of the fixed blood in contact with the plate (PL) various debris that may be to absorb.

Thus, the washing step (S600) may be performed before or after or before and after both the time of the step (S300) for delivering dye sample after the blood is fixed to the plate (PL), a reaction zone as described above. Or washing step (S600) it is also possible to be performed before or after or before and after the time amount of the buffer stage (S800). Or washing step (S600) may be performed before or after or before and after the amount of time of the image pickup step (S400).

Step (S800) using a buffer patch (PA) to provide the desired environment in the reaction zone, wherein the buffer patch (PA) the reaction between the target substance in the dyed sample and the blood in contact with the plate (PL) It may be to facilitate is made. Buffer patch (PA) may be performed after the step (S300) in which the dyed sample is delivered, the buffer patch (PA) is in contact with the reaction zone, the plate (PL) and patch the buffer patch on (PA) water film (WF) whose primary component is a buffer solution stored in (PA) are formed, the water film is of intelligent pH for the reaction between the dyed sample and the target material, and wherein the dye sample and the target material is within the water film in the reaction it can be in the indicated pH conditions. In the dyeing of a target substance by staining the sample can be made smoothly along.

Here, although described as a buffer stage (S800) and the washing step (S600) is performed by each buffer patch (PA) and wash patch (PA) in a separate step, respectively, the two steps with a washing function and a buffer function It may be performed with a single patch (PA).

On the other hand, in this embodiment, the staining of the blood, washing the reaction zone, such as provided in a predetermined environment of the reaction region not necessarily have to be performed using the patch (PA). In other words, some of these processes may be carried out using a solution necessary for the process, rather than the patch (PA). For example, the washing of the reaction area can be performed by spraying a washing solution to the reaction zone instead of contacting the wash patch (PA) to the reaction zone.

Staining of the blood in this embodiment, the washing of the reaction area, when and the like provided in a predetermined environment of the reaction zone to perform through the contacting patch (PA), is less than it is done through that the direct injection of a solution an amount of as a solution or a sample it is possible to complete the process, it is possible to obtain economical effects. Also, more precise, so controlling the degree of degree, the degree of cleaning, buffering of staining than direct injection patches (PA) for storing a variety of solutions to the plate (PL) solution, can be easily and avoid overreaction it is possible to perform the process may be as a result improve the quality of dyeing.

Figure 50 to Figure 52 are diagrams relating to the image taken at a blood test method according to the simple staining according to the present application.

Above danil dye is generally detects the bacteria in the blood, or may be used to determine the degree of infection, the degree of seongjeong bacteria. Referring to Figure 50, using the crystal violet staining in the sample can be stained for E. coli in the blood. If again, see Figure 51, it allows us to stain the bacteria diphtheria (Corynebacterium) in the blood using the methylene blue dye in the sample. Thus, in order to detect the bacteria it can be used to sample a basic dye mainly stained nuclei for the red blood cells and separated in the blood. However, it is possible to be sure that it is not used for staining samples or neutral dye stained samples psoriatic depending on the destination.

In the above-described single dye it may be used in addition to the general blood test (CBC). Referring to Figure 52, it allows us to stain the white blood cells in the blood using the methylene blue dye in the sample. It is possible to quantify the number of white blood cells in the blood through. If again, see Figure 53, it allows us to stain the blood cells in the blood by using the eosin stained samples. In this case, white blood cells, as well as all there is to the cytoplasm, such as red blood cells or platelets staining, each of the distinct blood can be carried out through image analysis, which is based on the shape and size of a blood cell. After each blood cell are distinguished, it is possible to quantify each of the blood cell types in the blood. This is a general blood test to be completed.

Hereinafter, description will be made to the embodiment of the patch (PA) that can be used in the blood test of the present embodiment. Each patch (PA) is described as storing several components, each component may be understood as the above-described base material (BS) or additive material (AS). However, components described as being able to be stored for each patch (PA) are not the whole component stored in each patch (PA), each patch (PA) may be stored, and with the additional components which are not specified.

4.5.1.1 dyed patch

Blood tests of the present application, may be carried out using a dye patch (PA) to store the dye sample. In other words, the patch (PA) may be reacted with the target substance in the blood storing the dye sample to stain it, and pass the sample dyed with a plate (PL).

The stained sample can be added to the substance (AS) stored in the patch (PA). In other words, the patch (PA) may be stored, and the solution containing the dye sample. In addition, the dye samples are stored in the patch (PA) is separate from the base material (BS) or the additive material, which, with the dyeing sample or the stained sample contained solution, the dyed sample is to be easily coupled to the target substance in the blood ( may be stored, and the AS).

The dyed sample may be a substance which combines color-developing mainly electrochemically in the target material. An example of a dye sample, and the sample is a basic dye, a neutral dye sample, such as acid-dyed sample, and thus detailed description thereof will be omitted here, because the above description.

As in the present embodiment, when to store the dye sample in the patch (PA) to pass to the plate (PL), some staining samples of blood and unreacted fixed to the plate (PL) is again the patches (PA) to be absorbed. In this way, and the washing process can be omitted, and in some cases can be also re-use of the patch (PA), can be a rapid and efficient diagnostic realized.

Patches (PA) according to an embodiment of the present application, provides a dyed sample, and a net structure to form the micro-cavity in which the dyed sample is stored for the reaction with the target material is stored in the contact with the reaction zone of the blood position staining the sample storage including a net structure (NS) passing a portion of the stained sample to the reaction zone may be a patch (PA).

Washington Patch 4.5.1.2

Blood test method according to the present embodiment, it may be performed using a wash patch (PA) to absorb the residual water. That is, blood test method according to the present embodiment, it is the residual water can be absorbed by the above was in contact with the plate (PL) separating said wash patch (PA). The residue may be the above-described dye patch (PA) to when the contact has been separated to the plate (PL), means a residue which is not removed by absorption in each patch (PA).

The wash patch (PA) may be stored, and the, washing solution. The wash solution may be TBS and PBS with tween-20, some added. The wash solution, depending on the residue to the absorption, may be provided in a solution in which the residue can be dissolved. In addition, the patch (PA) is stored in the washing solution may be stored, and the base material (BS) or additive material (AS) to assist in the further washing.

Save the wash solution to the patches (PA), said patch (PA), by separating the above it was in contact with the plate (PL), said plate (PL) impurity to the residue of, for example, which have dye samples and other foreign matter not bind It can be removed by absorption or the like.

The wash patch (PA) that is to absorb the residual water, the wash patch (PA) the plate (PL), that is, by being brought into contact with the plate (PL) area in which the blood where the water film (WF) is formed and, there is the residue may be dissolved in the water film (WF). The water film is the above residue was dissolved in (WF), the wash patch (PA) is the wash patch (PA by moving the water film (WF) is hooked to the washing patch (PA) when separated from the plate (PL) ) it can be absorbed into.

4.5.1.3 patch buffer (buffer patch)

Blood test method according to the present embodiment, the buffer may be performed using the patch (PA). In other words, the patch buffer (PA), can store the buffer and deliver the desired environment in the plate (PL). The buffer patch (PA) is capable of storing a buffer solution in which each phase of the blood to be smoothly performed. Buffer solution may be a buffer solution having the indicated pH required for the staining is mainly purpose.

4.5.2 Criteria Example 2 - Romanov ski dye

Blood tests in accordance with one embodiment of the present application, using a plate (PL) and the patch (PA) can be carried out by Romanov ski staining.

Figure 54 shows a flowchart illustrating a blood test method according to the Romanov ski dye as another example of the blood test method according to the present application.

Blood test method according to the Romanov ski dye according to one embodiment of the present application, taking an image of the step (S300), staining for placing the blood into the reaction area (S200), passes the dye sample to the reaction area a step (S400), and images that can be analyzed, including the step (S500) of performing a blood test.

Transferring the dye from the blood sample by Romanov ski dye (S300) is passed at least two or more dye in the blood sample. In this embodiment, for this purpose it can be carried out mainly by using a plurality of dye patches (PA) for storing one of a plurality of stained samples, respectively. However, in the following two dye patch (PA) for using the two dye samples for convenience of description to stain the blood, that is based on the use of the first dye patch (PA) and the second dye patch (PA) It will be described. However, not necessarily that the number of dye patches (PA) restricted to two in the present embodiment, it is also possible to use three or more. Modification using the three or more dye patches (PA) in the following description because the invention can be applied without thinking to those skilled in the art will be understood to be included in this embodiment.

Step (S300) to pass stained sample before contacting the first dye patch (PA) to store the dye sample to the reaction area on the plate (PL), such as a slide glass (S330), the second stain the sample is the reaction to be able to move to a region may comprise a second patch step (S350), step (S360) of separating the first patch (PA) from the reaction zone by contacting the (PA) to the reaction zone.

55 is a view illustrating a process in which a first dyeing the sample is delivered by the blood test method according to the Romanov ski dyeing according to the present application, Figure 56 is a second dye sample in a blood test method according to the Romanov ski dyeing according to the present application It is a view showing a process of transmission.

When Figure 55 and Figure 56, the first dye patch (PA) is, The first store dyed sample, and passes the sample to stain the blood located on the plate (PL) (S330). Then, when the first blood sample is stained sufficiently passed, the first to separate the dye patch (PA) from the reaction zone (S340). Next, a second dye patch (PA), the second stores the stained sample and delivering the sample to stain the blood located on the plate (PL) (S350). If after a second sample is stained blood sufficiently transmitted to the second to separate the dye patch (PA) from the reaction zone (S360).

Here, the first dye and the second sample dyed samples may be stained with different target substances in the blood. For example, the first staining the sample is any one of acid dye staining a sample of the basic dye-dyed sample and the cytoplasm to the nucleus, the second dye sample may be a different one. Or vice versa. Specifically, the first staining the sample may be of methylene blue, and the second sample is stained eosin. Of course, the first dye and the second sample puts out a the type of the dye sample but not limited because of the above-described example.

Referring again to Figure 55, if the first dye patch (PA) is in contact with the reaction zone, the first dye can stain the sample to a first target material. Referring back to Figure 56, when the second dye patch (PA) is in contact with the reaction zone, the two may be dyed sample is dyed a second target material. Here, the first target material may be any one of a cell nucleus and cytoplasm, and the second target material is the other of the nuclei and cytoplasm.

Since dyed sample is already described in the above-mentioned step S310 for the process of dyeing a target material, the detailed description thereof will be omitted.

Referring again to Figure 55 and Figure 56, the first dye patch (PA) and the second dye patches (in the course of the PA is separated for each reaction zone, the water film (WF) formed in the neighboring contact areas stained patches (PA) there is absorption in, this time without reaction in the blood can be absorbed by the remaining excess dye sample is stained patches (PA). stained patches (PA) that is so explained in S320 the above-described steps with respect to absorbing the residual dye sample , it will be also omitted in this case a detailed explanation of this.

May When dyeing is completed, the analysis of an image obtained by capturing an image of the reaction zone of the plate (PL) obtaining a dye image (S400), performing a blood test (S500).

In step (S120), washing the patch (PA) for fixing the step (S100), the smeared blood is a blood test method according to the simple staining in accordance with one embodiment of the present application, smear the blood on the aforementioned plate (PL) a it is at least one of the steps (S800) to provide the desired environment in the reaction zone by a step (S600), and buffer patch (PA) washing the reaction zone may further include using. Here, step S100 and step S120 may be performed before the time point of step S200. Also here, step S600 and step S800 may be performed at least once at the time between each step S200 and step S400.

Step (S600) of washing the reaction zone using the wash patch (PA), can wash the patch (PA) is intended to absorb the residual water in contact with the plate (PL). This step has already described in a blood test method according to the simple staining in accordance with the present invention. However, between the steps (S340) and the second dye patches step (S350) of contacting the (PA) to the reaction zone for separating the washing step (S600) includes a first dye patch (PA) in the present embodiment from the reaction zone, 2 may be performed after separating the dye patch (PA) from the reaction zone (S360), or the amount of time.

Step (S800) using a buffer patch (PA) to provide the desired environment in the reaction zone, wherein the buffer patch (PA) the reaction between the target substance in the dyed sample and the blood in contact with the plate (PL) It may be to facilitate is made.

This step S800 is, the second buffer having a first buffer solution having the indicated pH for staining of the stained sample to point pH for that establishes a first buffer patch (PA) and the second dye samples of the dyeing reaction the second buffer can be carried out using the two patches (PA) of the patch (PA) to store. That is, step S800 is, using the first buffer patch step (S810) and a second buffer patch (PA) by using the (PA) providing a first environment for the first staining with the first stain sample of 2 It may include a step (S820) for providing a second environment for the second staining with the dye sample. Here, the performed after the first buffer stage (S810) and a second buffer stage (S820) the separation step of the first dye patch (PA) the separating step (S340) and the second dye patch (PA), respectively (S360) It can be.

Or the step S800 is or may be performed through a single patch buffer (PA). At this time, the buffer step (S800) may be performed in at least one point in time after the step S340 and step S360.

Here, although described as a buffer stage (S800) and the washing step (S600) is performed by each buffer patch (PA) and wash patch (PA) in a separate step, respectively, the two steps with a washing function and a buffer function It may be performed with a single patch (PA).

Figure 57 and Figure 58 is a diagram of the image imaged by the ski Romanov blood test method of the dye according to the present application.

Compared to Figure 57 and 58, 57 is directed to a staining buffer is omitted, a step (S800), Fig. 58 relates to the staining buffer, perform the step (S800) after step S360. When using the two dyed sample it is because there may be reduced staining quality of the blood due to the interference between the dye sample, step S360 after which the two or more stained sample into the reaction zone there is to be preferred that a buffer stage (S800) performed can.

59 is a view showing a process in which a first dye and a second sample dyed sample is passed along in the blood test method according to the Romanov ski dyeing according to the present application.

On the other hand, the above was described as using a plurality of dye patches (PA) comprises one of a plurality of stained samples, each performing a blood test. However, even if the blood stain that requires a plurality of samples stained as shown in Figure 59 it is also possible to use only one dye patch (PA).

For example, access to the first dye patch, the second dye patch (PA) to store (PA) and eosin for storing methylene blue as in the present embodiment to perform the Romanov ski stained using methylene blue and eosin respectively, but, alternatively after which stores one dye patch (PA) of methylene blue and eosin are mixed Kim jaaek or light amount or the like, it is also possible to similarly stained blood and simple dyeing.

However, In this case, because there is interference (precipitation) between the dyed samples stored in the dyeing patch (PA) can take place, it may be desirable that this buffering operation accompanied after dyeing.

On the other hand, the above it it is also possible to at least one of the plurality of patches (PA) a plurality of patches, but contrast is described that each store a single dye (PA) is stored in the dye material of the plurality of. For example, the write-perform gimja dye-gimja claim to perform a dyeing first dye patch, saving the light liquid, and the second dye patches to save Kim jaaek, light by performing the steps according to the embodiment can do.

On the other hand, the above has been described on the basis of the Romanov ski staining with respect to this embodiment, the embodiment puts out that can be used universally for staining is at least more than one dye used.

On the other hand, in this embodiment, the staining of the blood, washing the reaction zone, such as provided in a predetermined environment of the reaction region not necessarily have to be performed using the patch (PA). In other words, some of these processes may be carried out using a solution necessary for the process, rather than the patch (PA). For example, the first dye is the use of a dye solution to dye the first receiving the sample, and the second dye may be carried out using a second dye patch (PA).

Staining of the blood in this embodiment, the washing of the reaction area, when and the like provided in a predetermined environment of the reaction zone to perform through the contacting patch (PA), is less than it is done through that the direct injection of a solution an amount of as a solution or a sample it is possible to complete the process, it is possible to obtain economical effects. Also, more precise, so controlling the degree of degree, the degree of cleaning, buffering of staining than direct injection patches (PA) for storing a variety of solutions to the plate (PL) solution, can be easily and avoid overreaction it is possible to perform the process may be as a result improve the quality of dyeing.

4.5.3 Based on Example 3 - Gram stain

Blood tests in accordance with one embodiment of the present application, may be carried out by Gram staining using a plate (PL) and the patch (PA).

60 is a flowchart illustrating a blood test method according to the Gram stain as another example of the blood test method according to the present application.

Blood by Gram stain in accordance with one embodiment of the present application method, recording an image on placing the blood into the reaction area (S200), transferring the dye sample to the reaction area (S300 '), staining a step (S400), and images that can be analyzed, including the step (S500) of performing a blood test.

Transferring the dye from the blood sample by Gram stain (S300 ') delivers the dyed sample, mordant sample, bleaching and counterstained specimen sample in the blood. In this embodiment, for this purpose it can be carried out mainly by using a plurality of patches (PA) for storing the dyed sample, mordant sample, bleaching sample and control at least one of the dye sample.

Here, each of the plurality of patches (PA) may store a single sample-related Gram stain. For example, the patch (PA) is the dye patch (PA), mordant mordant for storing the sample patches (PA), discolored patches to store the color of the sample (PA) and to store counterstained specimen to store the dye sample It may comprise a contrast dye patch (PA).

Further, where some of the Gram staining associated sample may be provided to the reaction zone in the form of directly injected instead, a solution provided to the reaction zone in the form as stored in the patch (PA). For example, the bleaching process can be performed by the bleach, instead of contacting the patch (PA) for storing the bleach to the reaction zone injected in the blood. Thus, if the forward dyeing process using a solution instead of the contact patch (PA) injection has some of the detailed steps of step S300 to be described later it can be changed to a solution injection step.

Also, where at least some of the plurality of patches (PA) is capable of storing a plurality of related Gram stain the sample. However, the sequence of the Gram stain should be made to the dyeing, mordanting, discoloration, the order of the contrast dye, mordant and bleaching has to be carried out with a time sequence. Considering this, for example, the sample and the dye mordant samples in a patch (PA) can be stored together.

However, the following description will be described in a plurality of patches (PA) for the convenience of description, each relative to that contains one gram stain associated sample.

Further comprising: in conjunction with Gram stain pass stained sample (S300 ') is a step (S310 to first contact the stain patch (PA) to the reaction regions on the same plate (PL) and the slide glass "), this stain patch from the reaction zone separating the mordant patch (PA) from the (reaction zone step (S320) step S330) contacting, mordant patch (PA) to the reaction zone, separating the (PA) (S340 '), color fading in the reaction zone patch step (S350 '), separating the color of the patch (PA) from the reaction zone (S360' comprising), counterstained in a reaction zone in contact patches (PA) (S370 ') and a reaction zone by contacting the (PA) from the contrast it may include the step (S380 ') for separating the dye patch (PA).

Figure 61 to 63 is a view showing the present dyeing, mordanting, bleaching, dyeing process control in the blood test method according to the Romanov ski dyeing according to the present application, respectively.

Referring to Figure 61, into contact with the dye patch (PA) to the reaction zone (S310 ') thereby passing the dye in the blood sample and to separate from the reaction zone (S310'). The reaction zone and the dye patch in the blood sample is passed through a water film (WF) between (PA) is capable of dyeing all of the positive material and a negative material for the dye sample. For example, the dye in the Gram stain may stain the gram-Yang Seonggyun and gram-negative bacteria in purple.

Contacting a mordant patch (PA) to the reaction zone (S330 ') thereby pass the mordant into the blood sample and to separate from the reaction zone (S340'). Through a water film (WF) between the reaction region and the patch (PA) a mordant sample delivered to the blood speech material for the dye sample and a strengthening or a bond with benign material for the dye sample opposed to the dyed sample and the dyed samples the bond between can be weakened. For example, in the Gram stain mordant it may strengthen the bond between the dye and the Gram Yang Seonggyun so. On the other hand, if, even though they are mordant according to the type of staining that a positive material is not discolored due to bleaching sample also so present, but the step S330 'and step S340', an essential step.

Referring to Figure 62, color of the patch, thus delivering the color of the sample of blood and to separate from the reaction zone (S360 (S350), into contact with (PA) to the reaction zone). Through a water film (WF) between the reaction region and the patch (PA) bleached samples transmitted to the blood causes discoloration speech material for the dye sample. That is, it is possible to separate the sound material and the dye for the dye.

Referring to Figure 63, "thereby delivering contrast dye in the blood sample and to separate from the reaction zone (S380 contacting a contrast dye patch (PA) to the reaction zone (S370) '). The reaction region and the patch (PA) the control passes to the blood through the water film (WF) between the dyed sample is then stained by combining them with audio material for the dye sample. For example, Gram stain control samples may be stained red with Gram-negative bacteria. On the other hand, it is also to be observed, if only the positive material for the dye according to the type of staining, step S370 'and step S380' is not an essential step.

May When dyeing is completed, the analysis of an image obtained by capturing an image of the reaction zone of the plate (PL) obtaining a dye image (S400), performing a blood test (S500).

In step (S120), washing the patch (PA) for fixing the step (S100), the smeared blood is a blood test method according to the simple staining in accordance with one embodiment of the present application, smear the blood on the aforementioned plate (PL) a it is at least one of the steps (S800) to provide the desired environment in the reaction zone by a step (S600), and buffer patch (PA) washing the reaction zone may further include using.

Here, the washing step (S600) and the buffer stage (S800) may be performed after the step S300 '. More specifically, it may be performed S600 and S800 are respectively the at least one point in time after the step S310 'to step S380', or between step S380 '.

4.6 Example of the blood test apparatus

Blood tests of the present application, may be performed by the blood test apparatus.

Figure 64 shows the blood test apparatus 10 according to one embodiment of the present application.

Blood test apparatus according to an embodiment of the present application may include a support plate 200, patch control unit 300 and the imaging unit 400. The Blood test apparatus according to this embodiment, includes a net structure (NS) forming the micro-cavity, and by using a patch that can hold the material in a liquid (SB) to said micro-cavity, staining the blood and dye image the imaging can be.

The support plate 200 can support a plate (PL) which the sample (SA) which is a diagnosis target in the reaction zone position.

Patch control unit 300 so that the dyed sample is passed to the reaction zone using at least one or more patches (PA) to be used in a blood test method according to an embodiment of the present invention described above for the reaction area of ​​the patch (PA) it is possible to control the relative position.

Image pick-up unit 400 may obtain the image of the blood stain by sensing the reaction zone.

More specifically, the image sensing unit 400 may include an image capture modyulreul. Here, the image acquisition module may include a camera module.

Accordingly, the image sensing unit 400 may obtain a specific image portion of the reaction zone. In the imaging unit 400 may assemble the parts by the image acquired.

Further, the blood test apparatus may further include a control unit 100.

The controller 100 has, and determines the image analysis program, and a blood test at least one may be installed in the program, and the type of blood cells from the blood image dye drives the installed programs, the presence of bacteria or the like, and counting the number, on this basis soaking blood, morphological information, or the presence or absence of bacteria, numerical, morphological generation information, and ultimately the patient's health or disease status, it can be determined, such as the progress of the disease.

Figure 65 shows an example of a patch, the controller 300 according to an embodiment of the blood test apparatus 10 according to the present application.

In blood test apparatus 10 according to one embodiment of the present application, the patch control section 300 may include a patch selection module 310 and the touch control module 330.

Patch selection module 310, it is possible to select the control target patch (PA). The The patch selection unit selecting the control target patch (PA), at least one dye patch to store dyed sample (PA), select various patches (PA) for storing the fixing solution, wash solution, a bleach, a mordant or a buffer solution It may be to.

Contact control module 330, may be to control a contact state of the selected patches (PA) and the reaction zone. To control the state of contact, it may be to control the relative position with respect to the reaction region of the patch (PA).

The above description is only to those described as the technical idea of ​​the present invention by way of example, those skilled in the art that various modifications, additions and substitutions will be possible without departing from the essential characteristics of the present invention. Thus embodiments of the invention described above, for example, are also possible to be implemented separately or in combination, it is another.

Accordingly, the disclosed invention embodiments is for illustrative and not intended to limit the technical idea of ​​the present invention, not by such an embodiment is the technical scope of the present invention is not limited. The scope of protection of the invention is to be interpreted by the following claims, all spirits within a scope equivalent will be construed as included in the scope of the present invention.

Claims (20)

  1. Dyeing dye staining the target specimen in the blood; And
    Is provided with a net structure to form the micro-cavity in which the dyed sample is stored, a net structure for delivering a portion of the stored stain sample contacts the reaction zone of the blood is positioned in the reaction region; containing
    Dyed patches.
  2. According to claim 1,
    Staining the sample for dyeing the dyed destination,
    Comprising at least one of an acidic dye sample, basic dyed sample and sample neutral dye
    Dyed patches.
  3. According to claim 1,
    The dyed samples, comprising a fluorescent dyed sample to the fluorescent dye to the target
    Dyed patches.
  4. According to claim 1,
    The dyed targets, including at least one of the blood cells, bacteria and parasites present in the blood,
    The dyed sample is dyed at least one of the cytoplasm, nucleus, and granules of the target dye
    Dyed patches.
  5. According to claim 1,
    And the dye is multiple target,
    The dyed sample, the first dye to the dye staining a first dyeing the target of the target sample, and a second dye for staining the sample to the second dye of the dye the target object
    Dyed patches.
  6. Comprises a net structure to form the micro-cavity and a blood test method using the patch to store dyed sample to stain the target dye in the blood to the minute voids, performing a blood test with the dye of the dye target,
    Placing the blood into the reaction area; And
    Using the patch for storing the dyed sample comprising: delivering the dye sample to the reaction zone; containing
    How a blood test.
  7. 7. The method of claim 6,
    Further comprising a; the method comprising: obtaining an image relating to the stained the blood by passing the dyed sample
    How a blood test.
  8. The method of claim 7,
    The target is a blood cell staining in the blood,
    Based on the image comprises obtaining at least one of type information and the number information and the morphological information of the blood cell; further comprising a
    How a blood test.
  9. The method of claim 8,
    Based on the obtained information CBC: performing the (CBC Complete Blood Count cell); further comprising a
    How a blood test.
  10. 7. The method of claim 6,
    The dyeing target, and the parasite or bacteria in the blood,
    Obtaining information about the presence of the parasite or bacteria, and information relating to the type, number information and morphological information, at least one of; further comprising a
    How a blood test.
  11. 11. The method of claim 10,
    Based on the obtained information of peripheral blood smear examination, comprising the steps of: performing the (CBC Complete Blood Count cell); further comprising a
    How a blood test.
  12. 7. The method of claim 6,
    Placing the blood,
    That the blood in the method for fixing the plates, carried out by the method or the sample to smear the sample on the plate as in any of the method for fixing to spread on the plate,
    How a blood test.
  13. 7. The method of claim 6,
    The method comprising delivering the dye sample to the reaction zone using the patch,
    Contacting the said patch to said reaction zone so that the dyed sample is moved to the reaction zone; And
    Includes; separating the patch from the reaction zone
    When the patch is separated from the reaction zone, the excess dye staining a sample of the sample fails to react with the dye the target is removed from said reaction zone,
    How a blood test.
  14. 7. The method of claim 6,
    Using a wash patch for storing a cleaning solution comprising: absorbing the foreign matter remaining on the excess dyed sample and the reaction zone from the reaction zone; further comprising a
    How a blood test.
  15. 7. The method of claim 6,
    The method comprising delivering the dye sample to the reaction zone using the patch,
    The method comprising using a first patch for storing the first staining the sample to stain the cytoplasm and nucleus of the dyeing any of the target the first transmission for staining the sample to the reaction zone; And
    Using a second patch for storing the second dye sample to the dye staining the other of the cytoplasm and the nucleus of the target step of the second pass stained sample to the reaction zone; containing,
    How a blood test.
  16. 16. The method of claim 15,
    Using a first buffer for storing the patch buffer solution comprising the steps of: providing a point pH of the reaction zone; further comprising a
    How a blood test.
  17. 17. The method of claim 16,
    Providing the indicated pH, on the at least one point in time after the method comprising the steps of: passing the first staining the sample and the second pass dyeing step between the time and the second staining samples to pass the sample carried out
    How a blood test.
  18. 7. The method of claim 6,
    The dye patch, storage of the first dye and the second dye sample to sample stained nucleus of the dye to dye the target object, and the cytoplasm of the dyeing,
    The method comprising delivering the dye sample to the reaction zone using the patch,
    So that the dye patch was stained with the cytoplasmic and nuclear staining of the target, to the reaction zone comprising the first dye and the sample passes the second samples stained
    How a blood test.
  19. 19. The method of claim 18,
    Wherein the first dye and the second sample after the step of forwarding the sample dye, using a buffer to store the patch buffer solution comprising the steps of: providing a point pH of the reaction zone; further comprising a
    How a blood test.
  20. Comprises a net structure to form the micro-cavity and a blood test apparatus using the patch to store dyed sample to stain the target dye in the blood to the minute voids, performing a blood test with the dye of the dye target,
    The reaction zone is where the plate support for supporting a plate to be the blood located in said reaction zone;
    Patch control unit for controlling the relative position of the reaction zone of a patch to deliver the dye sample to the reaction area using the patch for storing the dyed sample; And
    In order to check the blood reaction detector for detecting the result of the dye in the target blood stain; containing
    Blood test equipment.
PCT/KR2017/002030 2016-02-23 2017-02-23 Blood staining patch, and method and apparatus for testing blood using same WO2017146506A1 (en)

Priority Applications (16)

Application Number Priority Date Filing Date Title
US201662298959P true 2016-02-23 2016-02-23
US62/298,959 2016-02-23
KR10-2016-0069938 2016-06-04
KR1020160069937A KR20170099738A (en) 2016-02-23 2016-06-04 Contact-type staining patch and manufacturing method thereof
KR1020160069936A KR20170099737A (en) 2016-02-23 2016-06-04 Contact-type staining patch and staining method using the same
KR1020160069938A KR20170099739A (en) 2016-02-23 2016-06-04 Contact-type staining-assist patch, manufacturing method thereof and staining method using the patch
KR10-2016-0069937 2016-06-04
KR10-2016-0069936 2016-06-04
KR10-2016-0095739 2016-07-27
KR1020160095739A KR20170099741A (en) 2016-02-23 2016-07-27 Test kit
KR10-2016-0118462 2016-09-13
KR1020160118462A KR20170099742A (en) 2016-02-23 2016-09-13 Test kit and staining method using the same
KR10-2016-0144551 2016-11-01
KR1020160144551A KR20170099745A (en) 2016-02-23 2016-11-01 Method for diagnosis and device perfoming the same
KR10-2017-0024391 2017-02-23
KR1020170024391A KR20170099788A (en) 2016-02-23 2017-02-23 Blood stainig patch, hematologic diagnostic method using the patch and device using the same

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
CA3015598A CA3015598A1 (en) 2016-02-23 2017-02-23 Blood staining patch, method and device for blood test using the same
EP17756844.1A EP3428644A1 (en) 2016-02-23 2017-02-23 Blood staining patch, and method and apparatus for testing blood using same
CN201780025111.XA CN109073629A (en) 2016-02-23 2017-02-23 Blood dyes patch and makes the method and apparatus for using it to test blood
JP2018562490A JP2019510238A (en) 2016-02-23 2017-02-23 Blood stained patches, methods and devices for blood tests to use it
BR112018017168A BR112018017168A2 (en) 2016-02-23 2017-02-23 Blood staining plaster, method and apparatus for using the same blood test
AU2017224479A AU2017224479A1 (en) 2016-02-23 2017-02-23 Blood staining patch, method and device for blood test using the same
US16/079,271 US20190056296A1 (en) 2016-02-23 2017-02-23 Blood staining patch, method and device for blood test using the same

Publications (1)

Publication Number Publication Date
WO2017146506A1 true WO2017146506A1 (en) 2017-08-31

Family

ID=59685451

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2017/002030 WO2017146506A1 (en) 2016-02-23 2017-02-23 Blood staining patch, and method and apparatus for testing blood using same

Country Status (1)

Country Link
WO (1) WO2017146506A1 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3870146A (en) * 1973-12-10 1975-03-11 Sci Med Lab Inc Wright{3 s stain packet
US4250257A (en) * 1978-08-24 1981-02-10 Technicon Instruments Corporation Whole blood analyses in porous media
US20050202567A1 (en) * 2003-03-24 2005-09-15 Zanzucchi Peter J. Analyte concentration detection devices and methods
KR100601831B1 (en) * 2002-09-13 2006-07-14 주식회사 엘지화학 Biochip prepared by gelation on a chip substrate
US20140038230A1 (en) * 2010-05-08 2014-02-06 Veridex, Llc A simple and affordable method for immunophenotyping using a microfluidic chip sample preparation with image cytometry

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3870146A (en) * 1973-12-10 1975-03-11 Sci Med Lab Inc Wright{3 s stain packet
US4250257A (en) * 1978-08-24 1981-02-10 Technicon Instruments Corporation Whole blood analyses in porous media
KR100601831B1 (en) * 2002-09-13 2006-07-14 주식회사 엘지화학 Biochip prepared by gelation on a chip substrate
US20050202567A1 (en) * 2003-03-24 2005-09-15 Zanzucchi Peter J. Analyte concentration detection devices and methods
US20140038230A1 (en) * 2010-05-08 2014-02-06 Veridex, Llc A simple and affordable method for immunophenotyping using a microfluidic chip sample preparation with image cytometry

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BECK, MARKUS ET AL.: "On-chip Sample Preparation by Controlled Release of Antibodies for Simple CD 4 Counting", LAB ON A CHIP, vol. 12, 2012, pages 167 - 173, XP055411785 *

Similar Documents

Publication Publication Date Title
US5159403A (en) Flow cell mechanism in flow imaging cytometer
Hain et al. Comparison of CCD, CMOS and intensified cameras
US5892539A (en) Portable emission microscope workstation for failure analysis
JP4067826B2 (en) The imaging system and an imaging method thereof
KR101108305B1 (en) Wafer defect detection system with traveling lens multi-beam scanner
US4694527A (en) Mask washing apparatus for production of integrated circuit
EP0758629A1 (en) Process for treating base to selectively impart water repellency, light-shielding member formed substrate, and production process of color filter substrate for picture device
US6665054B2 (en) Two stage method
US8833252B2 (en) Screen printing apparatus
JP4430601B2 (en) Lithographic apparatus and device manufacturing method
DE60020638T2 (en) A lithographic projection apparatus
US7245133B2 (en) Integration of photon emission microscope and focused ion beam
WO2005017489A2 (en) Film mapping system
JPS6472540A (en) Radiation microscope and method for detecting light emitted from defect in dielectric layer of integrated circuit device
JPH0795046B2 (en) Absorption measurement apparatus and method
WO2000067290A3 (en) Integrated microcolumn and scanning probe microscope arrays
JPH1183753A (en) Optical substrate inspecting device
US20070146693A1 (en) Method and apparatus for simultaneous high-speed acquisition of multiple images
WO1999004278A1 (en) Probe station adapter for backside emission inspection
JP2012504252A (en) Improved method and apparatus for microscopy with structured illumination
KR20100015312A (en) Substrates having improved ink adhesion and oil crockfastness
EP0424061B1 (en) Forming long arrays from short components
WO2015084125A1 (en) Block copolymer
US6870684B2 (en) Multi-wavelength aperture and vision system and method using same
CN1954204A (en) Improved inspection of TFT LCD panels using on-demand automated optical inspection sub-system

Legal Events

Date Code Title Description
ENP Entry into the national phase in:

Ref document number: 2018562490

Country of ref document: JP

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 3015598

Country of ref document: CA

NENP Non-entry into the national phase in:

Ref country code: DE

REG Reference to national code

Ref country code: BR

Ref legal event code: B01A

Ref document number: 112018017168

Country of ref document: BR

ENP Entry into the national phase in:

Ref document number: 2017224479

Country of ref document: AU

Date of ref document: 20170223

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 2017756844

Country of ref document: EP

ENP Entry into the national phase in:

Ref document number: 2017756844

Country of ref document: EP

Effective date: 20180924

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 17756844

Country of ref document: EP

Kind code of ref document: A1

ENP Entry into the national phase in:

Ref document number: 112018017168

Country of ref document: BR

Kind code of ref document: A2

Effective date: 20180822