US3864564A - Acquisition system for slide analysis - Google Patents

Acquisition system for slide analysis Download PDF

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Publication number
US3864564A
US3864564A US400915A US40091573A US3864564A US 3864564 A US3864564 A US 3864564A US 400915 A US400915 A US 400915A US 40091573 A US40091573 A US 40091573A US 3864564 A US3864564 A US 3864564A
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United States
Prior art keywords
pulse
signal
slide
specimen
producing
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Expired - Lifetime
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US400915A
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English (en)
Inventor
William J Adkins
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Bayer Corp
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Corning Glass Works
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Publication date
Application filed by Corning Glass Works filed Critical Corning Glass Works
Priority to US400915A priority Critical patent/US3864564A/en
Priority to CA198,268A priority patent/CA1024244A/en
Priority to ES425713A priority patent/ES425713A1/es
Priority to DE2442641A priority patent/DE2442641C2/de
Priority to GB4103974A priority patent/GB1476694A/en
Priority to JP49109885A priority patent/JPS5759596B2/ja
Priority to SE7412085A priority patent/SE391815B/xx
Priority to IT27667/74A priority patent/IT1027596B/it
Priority to FR7432391A priority patent/FR2272407B1/fr
Application granted granted Critical
Publication of US3864564A publication Critical patent/US3864564A/en
Assigned to CIBA CORNING DIAGNOSTICS CORP., A CORP. OF DE. reassignment CIBA CORNING DIAGNOSTICS CORP., A CORP. OF DE. ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: CORNING GLASS WORKS
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/59—Transmissivity
    • G01N21/5907—Densitometers
    • G01N21/5911—Densitometers of the scanning type
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V10/00—Arrangements for image or video recognition or understanding
    • G06V10/10—Image acquisition
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts

Definitions

  • a closed loop scanning and positioning system for 3 0 623 finding and positioning white blood cells includes a J 'f t t I t t 158 Field 61 Search 250/201, 222 PC; 356/39, mu ed "Mung Sys em W0 photoelectric sensors, two light sources and a logic 356/401235/92 318/565 577 circuit.
  • the logic circuit produces signals which are used to drive a microscope stage in x and y directions [56] References (med to cause the blood cell to be positioned within a small UNITED STATES PATENTS aperture.
  • This invention relates to a system for converting the optical image of a laboratory slide to electrical signals and more particularly to an acquisition system for bringing a specimen of interest on the slide into position for analysis.
  • a scanning unit in this case a TV. camera linearly sweeps a vidicon target subjected to intense illumination which passes through the smeared slide.
  • a second light sensing device receives light at the beginning of each scan of the rotating mirror. This light sensing device produces a synchronizing pulse which is applied to the logic circuit. If a cell is detected during a scan, the logic circuit compares the time occurrence of the acquisition of the cell to the time of the synchronizing pulse. In this manner the logic circuit produces signals which specify the direction in which the slide is to be moved along the x axis in order to center the cell in the viewing aperture.
  • FIG. 1 is an optical and electrical schematic diagram of the system of this invention
  • FIGS. 2A2D depict the acquisition of a blood cell
  • FIGS. 3A-3C show the electrical schematic of the logic circuit.
  • FIG. 1 shows the optics for a system for scanning and counting leukocytes on a blood smeared slide 10.
  • Light from the lamp 12 passes through lens 14, is reflected from fold mirror 16, and passes through condenser lens 18.
  • the light passes through the slide 10 and is collected by the objective lens 20.
  • a first beam splitter 22 reflects approximaterly 40 percent of the light to the automatic focus and acquisition system of which this invention is a part.
  • the remainder of light is reflected by mirrors 24 and 26 to an optical-to-electrical convertor which scans and analyzes a blood cell whose image is centered on the aperture 28.
  • the light image passing through the aperture 28 is applied to relay lens 30, to optical preprocessor 32 and to the compensator 34.
  • the cell image is applied to vidicon type TV. camera 36 which produces electrical signals which are ultimately converted into digital signals representing the characteristics of each blood cell. This conversion is more fully described in the aforementioned Cotter application.
  • a portion of the focused light from beam splitter 22 strikes a rotating multifaceted mirror 38.
  • Light striking mirror 38 forms an image of the slide which is parfocal with the image applied to the converter.
  • the rotating mirror reflects a portion of the slide image onto a light sensing device 40.
  • Light sensing devices 42 and 44 are used in the focusing system as more fully described in the copending Amos et al application.
  • a very narrow slice of the slide image is scanned as the mirror 38 rotates.
  • the light sensing device 40 produces an output proportional to the light absorption of the area of the slide being scanned. This signal is amplified and peak detected in the pulse shaping circuits 46. This circuit produces acquisition pulses indicating the detection of a blood cell on a slide only for signals above a given threshold.
  • a second light source 48 is focused on the rotating mirror 38 and the focused light is swept across a second light sensing device such as photocell 50.
  • the light sensing device 50 is adjusted so that the light from source 48 strikes it at a predetermined position in the scan. In this case, the light strikes photocell 50 in time relation to the beginning of a scan of the mirror across the slide.
  • the photocell 50 produces a synchronizing signal to mark the beginning of the sweep by a given mirror facet.
  • the present invention is directed to the system which centers an image of each blood cell on the slide onto the aperture 28.
  • the acquisition pulses and synchronizing pulses are applied to logic circuitry 52.
  • the logic circuitry utilizes these pulses to determine if a specimen of interest is in the field of view of one of the scanning segments. If so, the circuitry determines which way the stage must move in order to center the specimen in the aperture. As each facet of the rotating mirror sweeps the field, one of four signals is generated by the logic circuitry. If no specimen is encountered, a normal stage motion signal is generated. This causes the y positioning motor 54 to move the slide one step in the y direction so that another field of view can be scanned. This one step per mirror facet motion continues until a specimen is encountered by the acquisition sensor 40.
  • the directional signal indicates whether the specimen is to be moved to the right or left, i x direction, in order to center the cell on the aperture.
  • the x positioning motor 56 moves the slide in either the +x or the x direction. This motion continues until the logic circuitry produces a cell-centered signal. At this time all slide motion is halted, the cell specimen is centered in the aperture 28, and the vidicon 36 is enabled to allow it to make an analysis of the cell.
  • the stage motors 54 and 56 are stepping motors which move the stage a predetermined distance for each applied pulse.
  • FIGS. 2A-2D depict the image of a blood smeared slide. Included in this image is a specimen of interest, notably the blood cell 58.
  • the figures depict the image in relation to the aperture 28 and the figures depict the image in relation to the field of view which is scanned and which is shown as the shaded areas. (It should be remembered that the image applied to aperture 28 is parfocal with the image scanned by the rotating mirror 38. Therefore, it is accurate to depict the scan field of view as the shaded area in relation to the aperture 28 in this manner.)
  • FIG. 2A A first scan by one facet of the mirror 38 is depicted in FIG. 2A. During this scan no blood cell is detected by the acquisition detector 40. Therefore, the logic circuitry produces a normal stage motion signal which moves the slide by one increment in the y direction.
  • the acquisition detector 40 does not produce an acquisition pulse. Again a normal stage motion signal moves the slide one increment in the ydirection to the position depicted in FIG. 2C. During this scan the acquisition detector 40 produces a pulse indicating that a blood cell lies in the field of view of this scan. Further, the logic circuitry detects that the cell lies to the left of the aperture 28. Therefore, the logic circuitry produces a directional signal which moves the slide in the +x direction. This directional signal is produced until the slide is positioned with the cell 58 centered on the aperture 28 as depicted in FIG. 2D.
  • FIGS. 3A-3C The logic circuitry for producing signals in this manner is shown in FIGS. 3A-3C.
  • a synchronizing pulse from the synchronizing detector 50 is applied to set the flip-flop 60 at the beginning of each scan by a mirror facet. If there are more than one sync pulse during a scan, due to noise, only the first sync pulse sets the flipflop 60.
  • the flip-flop 60 triggers the one-shot multivibrator 62. By adjusting the monostable time period of multivibrator 62, it is possible to center the aperture with respect to the viewing binoculars used by the operator.
  • One-shot multivibrator 62 triggers the one-shot multivibrator 64 which produces a pulse of predetermined width. This is used to set the flip-flop 66. When the flipflop 66 is set an oscillator 68 is turned on. This triggers clock pulses which are applied to one-shot multivibrator 70 which produces pulses ofa predetermined width. These pulses are applied to the binary counters 72 and 74. The outputs of the counters 72 and 74 are decoded by the decoding gates 76 and 78. The decoding circuitry divides each scan line into 60 equal increments. A center pulse is produced at the middle of the scan and an end pulse is produced at the end of the scan.
  • an acquisition pulse occurs during a scan, it passes through AND gate 84 and inverter 86 to set the acquisition flip-flop 88. If an acquisition pulse occurs at a time which coincides with the center pulse, the center pulse passes through AND gate 94 which is enabled by the acquisition pulse from gate 84. The output of gate 94 passes through inverter 96 to set the cell-centered flipflop 98. Flip-flop 98 enables the AND gate 100 and the end pulse passes through it to produce the cellcentered pulse.
  • a directional signal is produced which indicates the direction of movement along the scan required to center the acquisition pulse.
  • a shift register which includes a first stage flip-flop 102 and a second stage flip-flop 104.
  • the first stage flip-flop is initially set; it is reset by the center pulse.
  • the state of the first stage flip-flop 102 is set into the second stage flip-flop 104. If the acquisition pulse occurs before the center pulse, a set state is transferred from the flip-flop 102 to flipflop 104.
  • a reset condition is transferred from flip-flop 102 to flipflop 104. If an acquisition has occurred, the end pulse will pass through AND gate 106 and interrogate the AND gates 108 and 110. If the acquisition occurs before the center pulse, the flip-flop 104 is set so that the connection from flip-flop 104 to the gate 110 is high. This allows the pulse from gate 106 to pass through gate 110 to produce a directional signal indicating a move in the +x direction. Conversely, if the acquisition pulse occurs after the center pulse, the flipflop 104 is reset and the inverter 112 applies a high condition to the gate 108. The pulse from gate 106 passes through the gate 108 to produce a directional signal indicating a move in the x direction.
  • the acquisition and cell-centered flip-flops 88 and 98 will still be reset at the time of the end pulse.
  • the bottom outputs of these flip-flpos acts through OR gate 90 to enable the AND gate 92.
  • the AND gate 92 passes the end pulse to produce a normal stage motion signal.
  • Priority circuitry is connected so that only one of the cell-centered, directional, and normal stage motion signals can be produced to the exclusion of the others. With the circuitry described thus far, it would be conceivable that all three signals would be produced at the end of a given scan. Of course it is necessary that only one produced so as to avoid confusion.
  • the highest priority signal is the cell-centered signal.
  • the flipflop 98 When the flipflop 98 is set, the lower output from this flip-flop is low. This acts through the gate 114 to hold the acquisition flip-flop 88 in the reset condition. This prevents an end pulse from passing through AND gate 106 to produce a directional signal.
  • the bottom output of flip-flop 98 which is low because the flip-flop is set, acts through OR gate 90, to block AND gate 92. This prevents the production of a normal stage motion signal.
  • This manual adjustment moves the center pulse in relation to the sync pulse. It is desirable that the center pulse occur with respect to the scan at a point which is approximately in the middle of the field of view of the camera 36.
  • the one-shot multivibrator 62 can be adjusted until this condition is met.
  • the frequency adjustment of the oscillator 68 can be adjusted. By lowering the frequency of these pulses, the si tty clock pulses will encompass a greater length of scan. Similarly, increasing the frequency decreases the length of scan.
  • conversion means producing said electrical output representing the optical characteristics of said slide
  • said light sensing device producing an output representing the light absorption of the area of the slide being scanned
  • a pulse shaping means connected to the output of said light sensing device and producing an acquisition signal when the area of the slide being scanned contains a specimen of interest
  • syn- 10 chronizing means producing a synchronizing signal at a predetermined point in each scan, and logic circuitry responsive to said synchronizing signal and to said acquisition signal for producing control 5 signals, said control signals being used to control said positioning mechanism to position said slide so that the image of a specimen of interest is centered on said conversion means.
  • a rotating mirror a light source focused on said rotating mirror, and a second light sensing device, said second light sensing device and said light source being positioned so that reflected light striking said second light sensing device is in time relation to the beginning of a scan of said mirror across said slide, said second light sensing device producing a synchronizing signal upon reception of said light.
  • said logic circuitry includes means for producing a normal stage motion signal if no specimen is detected during a scan said normal stage motion signal being applied to said positioning mechanism to move said slide by an increment in a direction orthogonal to the direction of scan. 4. The system recited in claim 1 wherein said logic circuitry includes:
  • a decoder the outputs of said counter being applied to said decoder, said decoder producing said center pulse upon the occurrence of a predetermined count in said counter.
  • a shift register having a first stage which is set by said synchronizing signal and which is reset by said center pulse and a second stage which is switched by said acquisition signal, said first stage being con nected to said second stage so that the state of said first stage is set into said second stage when said acquisition signal occurs, the output of said second stage being a bistable directional signal which indicates whether said acquisition signal occured before or after said center pulse.
  • an acquisition flip-flop said acquisition flip-flop being set by said acquisition signal
  • said end pulse and the output of said acquisition flip-flop being applied to said second gate to produce a directional signal, said directional signal being applied to said positioning mechanism to move said slide along the direction of scanning required to center said specimen on said aperture.
  • priority circuitry connected to enable said logic circuitry to produce only one of said specimen- .centered, directional and normal stage motion signals to the exclusion of others in the order of priority of, a specimen-centered signal, a directional signal, and a normal stage motion signal.
  • an adjustable one-shot multivibrator said synchronizing pulse being applied to trigger said one-shot multivibrator, the output of said one-shot multivibrator being applied to said oscillator to start the production of said clock pulses, said one-shot multivibrator having an adjustable monostable time period so that said center pulse occurs at a position in said scan which coincides with the center of the field of view of said conversion means.
  • said means for projecting includes a beam splitter which projects one slide image to said conversion means and another slide image to said rotating mirror.
  • a second light sensing device said second light sensing device and said light source being positioned so that reflected light striking said second light sensing device is in time relation to the beginning of a scan of said mirror across said slide, said second light sensing device producing a synchronizing signal upon reception of said light.

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  • Engineering & Computer Science (AREA)
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  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
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US400915A 1973-09-26 1973-09-26 Acquisition system for slide analysis Expired - Lifetime US3864564A (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
US400915A US3864564A (en) 1973-09-26 1973-09-26 Acquisition system for slide analysis
CA198,268A CA1024244A (en) 1973-09-26 1974-04-26 Acquisition system for slide analysis
ES425713A ES425713A1 (es) 1973-09-26 1974-04-26 Un metodo y aparato perfeccionados para ajustar la posicionde especimenes en las diapositivas analiticas para analisis microscopico.
DE2442641A DE2442641C2 (de) 1973-09-26 1974-09-06 Anordnung zur Einstellung mikroskopischer Objekte
GB4103974A GB1476694A (en) 1973-09-26 1974-09-20 Acquisition system for slide analysis
JP49109885A JPS5759596B2 (enrdf_load_stackoverflow) 1973-09-26 1974-09-24
SE7412085A SE391815B (sv) 1973-09-26 1974-09-25 Apparat for instellning av ett prov for mikroskopisk undersokning
IT27667/74A IT1027596B (it) 1973-09-26 1974-09-25 Sistema di acquisizione per l ana lisi di vetrini
FR7432391A FR2272407B1 (enrdf_load_stackoverflow) 1973-09-26 1974-09-26

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US400915A US3864564A (en) 1973-09-26 1973-09-26 Acquisition system for slide analysis

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US400915A Expired - Lifetime US3864564A (en) 1973-09-26 1973-09-26 Acquisition system for slide analysis

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US (1) US3864564A (enrdf_load_stackoverflow)
JP (1) JPS5759596B2 (enrdf_load_stackoverflow)
CA (1) CA1024244A (enrdf_load_stackoverflow)
DE (1) DE2442641C2 (enrdf_load_stackoverflow)
ES (1) ES425713A1 (enrdf_load_stackoverflow)
FR (1) FR2272407B1 (enrdf_load_stackoverflow)
GB (1) GB1476694A (enrdf_load_stackoverflow)
IT (1) IT1027596B (enrdf_load_stackoverflow)
SE (1) SE391815B (enrdf_load_stackoverflow)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3970841A (en) * 1974-11-25 1976-07-20 Green James E Method and apparatus for dual resolution analysis of a scene
US3970845A (en) * 1975-10-23 1976-07-20 Corning Glass Works Pulse discriminator circuit
US4000417A (en) * 1975-08-25 1976-12-28 Honeywell Inc. Scanning microscope system with automatic cell find and autofocus
DE2720036A1 (de) * 1976-05-04 1977-11-24 Green James E Verfahren und vorrichtung zur bildanalyse unter doppelaufloesung
US4125828A (en) * 1972-08-04 1978-11-14 Med-El Inc. Method and apparatus for automated classification and analysis of cells
US4449084A (en) * 1981-12-28 1984-05-15 University Of Pittsburgh Positioning servo-mechanism or tachometer employing self-scanning light sensing array
WO1985000657A1 (en) * 1983-07-22 1985-02-14 N. P.-New Products Nordinvent Investment Aktiebola Method and arrangement for the contact-free quality identification and structural description of, for instance, workpieces such as lengths of timber
US4622502A (en) * 1983-05-17 1986-11-11 Matsushita Electric Industrial Co., Ltd. Position detecting apparatus
US5323012A (en) * 1991-08-16 1994-06-21 The Regents Of The University Of California Apparatus for positioning a stage
US5606410A (en) * 1993-11-04 1997-02-25 Compagnie Generale Des Matieres Nucleaires Method for controlling the surface state of one face of a solid and the associated device
US6144118A (en) * 1998-09-18 2000-11-07 General Scanning, Inc. High-speed precision positioning apparatus
EP2856117A4 (en) * 2012-05-29 2016-02-17 Univ Macquarie BIDIRECTIONAL SCANNING FOR LUMINESCENCE MICROSCOPY
CN108227170A (zh) * 2016-12-12 2018-06-29 凝辉(天津)科技有限责任公司 一种用于显微镜的二维扫描装置

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02116998U (enrdf_load_stackoverflow) * 1989-03-06 1990-09-19

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2859653A (en) * 1955-02-21 1958-11-11 Servo Corp Of America Wide-angle prism scanner
US3466514A (en) * 1967-06-26 1969-09-09 Ibm Method and apparatus for positioning objects in preselected orientations
US3515877A (en) * 1965-12-13 1970-06-02 John W Massing Electro-optical positioner
US3541338A (en) * 1967-01-12 1970-11-17 Ibm Positioning system

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2859653A (en) * 1955-02-21 1958-11-11 Servo Corp Of America Wide-angle prism scanner
US3515877A (en) * 1965-12-13 1970-06-02 John W Massing Electro-optical positioner
US3541338A (en) * 1967-01-12 1970-11-17 Ibm Positioning system
US3466514A (en) * 1967-06-26 1969-09-09 Ibm Method and apparatus for positioning objects in preselected orientations

Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4125828A (en) * 1972-08-04 1978-11-14 Med-El Inc. Method and apparatus for automated classification and analysis of cells
US3970841A (en) * 1974-11-25 1976-07-20 Green James E Method and apparatus for dual resolution analysis of a scene
US4061914A (en) * 1974-11-25 1977-12-06 Green James E Method and apparatus for dual resolution analysis of a scene
US4000417A (en) * 1975-08-25 1976-12-28 Honeywell Inc. Scanning microscope system with automatic cell find and autofocus
DE2637496A1 (de) * 1975-08-25 1977-04-14 Honeywell Inc Verfahren und vorrichtung zum automatischen betrieb eines mikroskopes
US3970845A (en) * 1975-10-23 1976-07-20 Corning Glass Works Pulse discriminator circuit
DE2720036A1 (de) * 1976-05-04 1977-11-24 Green James E Verfahren und vorrichtung zur bildanalyse unter doppelaufloesung
FR2350596A2 (fr) * 1976-05-04 1977-12-02 Green James E Procede et appareillage pour l'analyse d'un champ selon deux resolutions
US4449084A (en) * 1981-12-28 1984-05-15 University Of Pittsburgh Positioning servo-mechanism or tachometer employing self-scanning light sensing array
US4622502A (en) * 1983-05-17 1986-11-11 Matsushita Electric Industrial Co., Ltd. Position detecting apparatus
WO1985000657A1 (en) * 1983-07-22 1985-02-14 N. P.-New Products Nordinvent Investment Aktiebola Method and arrangement for the contact-free quality identification and structural description of, for instance, workpieces such as lengths of timber
US5323012A (en) * 1991-08-16 1994-06-21 The Regents Of The University Of California Apparatus for positioning a stage
US5606410A (en) * 1993-11-04 1997-02-25 Compagnie Generale Des Matieres Nucleaires Method for controlling the surface state of one face of a solid and the associated device
US6144118A (en) * 1998-09-18 2000-11-07 General Scanning, Inc. High-speed precision positioning apparatus
US6744228B1 (en) 1998-09-18 2004-06-01 Gsi Lumonics Corp. High-speed precision positioning apparatus
US20040140780A1 (en) * 1998-09-18 2004-07-22 Cahill Steven P. High-speed precision positioning apparatus
US6949844B2 (en) 1998-09-18 2005-09-27 Gsi Group Corporation High-speed precision positioning apparatus
EP2856117A4 (en) * 2012-05-29 2016-02-17 Univ Macquarie BIDIRECTIONAL SCANNING FOR LUMINESCENCE MICROSCOPY
CN108227170A (zh) * 2016-12-12 2018-06-29 凝辉(天津)科技有限责任公司 一种用于显微镜的二维扫描装置

Also Published As

Publication number Publication date
DE2442641C2 (de) 1985-12-12
JPS5061290A (enrdf_load_stackoverflow) 1975-05-26
CA1024244A (en) 1978-01-10
FR2272407A1 (enrdf_load_stackoverflow) 1975-12-19
SE391815B (sv) 1977-02-28
FR2272407B1 (enrdf_load_stackoverflow) 1981-12-04
ES425713A1 (es) 1976-06-16
SE7412085L (enrdf_load_stackoverflow) 1975-03-27
GB1476694A (en) 1977-06-16
JPS5759596B2 (enrdf_load_stackoverflow) 1982-12-15
DE2442641A1 (de) 1975-03-27
IT1027596B (it) 1978-12-20

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Owner name: CIBA CORNING DIAGNOSTICS CORP., MEDFIELD, MASSACHU

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:CORNING GLASS WORKS;REEL/FRAME:004483/0427

Effective date: 19851105