US3862013A - Device for making a culture of micro-organisms - Google Patents

Device for making a culture of micro-organisms Download PDF

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Publication number
US3862013A
US3862013A US363593A US36359373A US3862013A US 3862013 A US3862013 A US 3862013A US 363593 A US363593 A US 363593A US 36359373 A US36359373 A US 36359373A US 3862013 A US3862013 A US 3862013A
Authority
US
United States
Prior art keywords
culture
culture medium
openings
organisms
micro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US363593A
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English (en)
Inventor
Joseph F Pagano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline Beecham Corp
Original Assignee
SmithKline Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SmithKline Corp filed Critical SmithKline Corp
Priority to US363593A priority Critical patent/US3862013A/en
Priority to GB2127274A priority patent/GB1421683A/en
Priority to AU69102/74A priority patent/AU485531B2/en
Priority to JP5723574A priority patent/JPS5721977B2/ja
Priority to DE19747417496U priority patent/DE7417496U/de
Priority to NLAANVRAGE7406751,A priority patent/NL178264C/xx
Priority to DE2424384A priority patent/DE2424384C2/de
Priority to CA200,399A priority patent/CA1030439A/en
Priority to IT23025/74A priority patent/IT1012701B/it
Priority to ES426509A priority patent/ES426509A1/es
Priority to CH707274A priority patent/CH599346A5/xx
Priority to FR7417944A priority patent/FR2230721B1/fr
Priority to LU70147A priority patent/LU70147A1/xx
Priority to BE144646A priority patent/BE815419A/xx
Application granted granted Critical
Publication of US3862013A publication Critical patent/US3862013A/en
Assigned to SMITHKLINE BECKMAN CORPORATION reassignment SMITHKLINE BECKMAN CORPORATION CHANGE OF NAME (SEE DOCUMENT FOR DETAILS). EFFECTIVE DATE: 03/04/82 Assignors: SMITHKLINE CORPORATION
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/54—Constructional details, e.g. recesses, hinges hand portable

Definitions

  • An elongated member supports a culture medium which has openings extending inwardly from the surface in one section of the culture medium, the other section being free of openings.
  • the elongated member is in a container which has an open end with means to seal the open end.
  • the prior art devices fail to provide for the fact that some organisms grow or produce products of metabolism better in the subsurface regions of the culture medium.
  • Group A B-hemolytic streptococci produces more hemolysin when growing subsurface.
  • this is taken care of by inoculating the cul-- ture medium using a wire loop or needle containing the micro-organism on its surface. Such inoculation is not practical for the doctor who uses the devices under discussion.
  • the doctors problem is solved by providing him with a device having openings in the culture medium to receive the microorganisms subsurface.
  • SUMMARY OF THE INVENTION is in a container which has an open end with means to seal the open end.
  • a section of the culture medium is free of openings.
  • FIG. 1 is an elevational view of a device of the invention
  • FIG. 2 is a top plan view of the culture support used in the device of FIG. 1 before the culture medium is added;
  • FIG. 3 is a top plan view (partially broken away) of the culture support of FIG. 2 with the culture medium in place;
  • FIG. 4 is an enlarged view (partially broken away) of the culture medium of FIG. 3 showing the openings;
  • FIG. 5 is a section taken on the plane indicated by the line 55 in FIG. 3;
  • FIG. 6 is a right hand end elevation of the device of FIG. I with the culture support removed.
  • a device I for making a culture of micro-organisms in accordance with the invention has a transparent container 2 of, for example, glass or a plastic such as an acrylic resin such as methyl mcthacrylate or a polycarbonate resin.
  • an elongated member 4 has an inner end 5 and a recessed portion 6 in opposed substantially flat faces 8 (only one being shown). Each recessed portion is intermediate the ends of the elongated member 4 and has multiple small protrusions 10 to facilitate the retention of a body 12 of a solid culture medium in the recessed portions.
  • the culture medium is preferably flush with the adjoining portions of faces 8.
  • a multiplicity of openings 14 are placed in the section of culture medium 12 adjacent the inner end 5 of elongated member 4. They extend from the surface inwardly to the member 4. Advantageously they are relatively small, for example, having a diameter in the range offrom about 0.1 mm to about 2.0 mm.
  • the openings are readily formed by a punching operation with an appropriate needle punch.
  • elongated member 4 are guided by opposed grooves l6, 16 in hollow guide member 18 which has a cylindrical portion 20 (FIG. 1) provided with ridges 22 which sealingly engage a cylindrical entrance portion 24 of container 2.
  • the inner end of guide member 18 has a pair of opposed brush members 28, 28 positioned adjacent grooves l6, 16 so as to engage elongated member 4 as it is inserted into container
  • Guide member 18 has a cylindrical entrance portion 32 which is engaged by a plug portion 34 (FIG. I) of member 4 having a peripheral ridge 36 to seal closed the end of guide member 18.
  • the outer end of elongated member 4 is in the form of a handle 38.
  • the culture medium will be selecteddepending upon the micro-organisms involved. Suitable solid culture mediums are well known to the art. Reference may be had, for example, to the culture mediums disclosed in U.S. Pat. No. 3,616,265 the disclosure of which is incorporated herein by reference. Agar culture mediums are preferred since their solidity is readily controlled by varying the percent of Agar. A typical culture medium for use in connection with Group A B-hemolytic streptococci as set forth below:
  • a device for making a culture of micro-organisms comprising:
  • said culture medium having a multiplicity of openings extending inwardly from the surface and having a diameter of from about 0.1 mm to about 2.0 mm,
  • a container for the elongated member having an open end
  • a device in accordance with claim 1 in which the openings extend inwardly to the elongated member.
  • a device in accordance with claim 1 in which the openings are all in one section of said culture medium and another section thereof is free of openings.

Landscapes

  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
US363593A 1973-05-24 1973-05-24 Device for making a culture of micro-organisms Expired - Lifetime US3862013A (en)

Priority Applications (14)

Application Number Priority Date Filing Date Title
US363593A US3862013A (en) 1973-05-24 1973-05-24 Device for making a culture of micro-organisms
GB2127274A GB1421683A (en) 1973-05-24 1974-05-14 Device for making a culture of microorganisms
AU69102/74A AU485531B2 (en) 1973-05-24 1974-05-17 A device for making a culture of microorganisms
DE19747417496U DE7417496U (de) 1973-05-24 1974-05-20 Vorrichtung zur herstellung einer mikroorganismenkultur
NLAANVRAGE7406751,A NL178264C (nl) 1973-05-24 1974-05-20 Diagnostische inrichting voor het kweken van microoerganismen.
DE2424384A DE2424384C2 (de) 1973-05-24 1974-05-20 Vorrichtung zur Herstellung einer Mikroorganismenkultur
JP5723574A JPS5721977B2 (it) 1973-05-24 1974-05-20
IT23025/74A IT1012701B (it) 1973-05-24 1974-05-21 Dispositivo per effettuare una coltura di microorganismi
CA200,399A CA1030439A (en) 1973-05-24 1974-05-21 Device for making a culture of micro-organisms
ES426509A ES426509A1 (es) 1973-05-24 1974-05-21 Un dispositivo para hacer un cultivo de microorganismos.
CH707274A CH599346A5 (it) 1973-05-24 1974-05-22
FR7417944A FR2230721B1 (it) 1973-05-24 1974-05-22
LU70147A LU70147A1 (it) 1973-05-24 1974-05-22
BE144646A BE815419A (fr) 1973-05-24 1974-05-22 Dispositif pour la preparation d'une culture de micro-organismes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US363593A US3862013A (en) 1973-05-24 1973-05-24 Device for making a culture of micro-organisms

Publications (1)

Publication Number Publication Date
US3862013A true US3862013A (en) 1975-01-21

Family

ID=23430839

Family Applications (1)

Application Number Title Priority Date Filing Date
US363593A Expired - Lifetime US3862013A (en) 1973-05-24 1973-05-24 Device for making a culture of micro-organisms

Country Status (12)

Country Link
US (1) US3862013A (it)
JP (1) JPS5721977B2 (it)
BE (1) BE815419A (it)
CA (1) CA1030439A (it)
CH (1) CH599346A5 (it)
DE (2) DE7417496U (it)
ES (1) ES426509A1 (it)
FR (1) FR2230721B1 (it)
GB (1) GB1421683A (it)
IT (1) IT1012701B (it)
LU (1) LU70147A1 (it)
NL (1) NL178264C (it)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3961696A (en) * 1975-10-20 1976-06-08 Smithkline Corporation Device for making a culture of CO2 requiring organisms
US5073495A (en) * 1988-10-21 1991-12-17 Large Scale Biology Corporation Apparatus for isolating cloned vectors and cells having a recovery device
US6063038A (en) * 1999-04-09 2000-05-16 Clmp, Inc. Devices and methods for collecting fecal antigen specimens

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2141136B (en) * 1983-06-08 1987-03-04 Basildon Moulding Company Limi Dip slide
IL112842A (en) * 1995-03-02 1998-06-15 Batec Bio Analytical Techn Ltd Bacteria detection device in the sample

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3589983A (en) * 1968-12-11 1971-06-29 Becton Dickinson Co Culture bottle assembly
US3616265A (en) * 1969-08-07 1971-10-26 Smith Kline French Lab Device for making a culture of microorganisms

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3589983A (en) * 1968-12-11 1971-06-29 Becton Dickinson Co Culture bottle assembly
US3616265A (en) * 1969-08-07 1971-10-26 Smith Kline French Lab Device for making a culture of microorganisms

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3961696A (en) * 1975-10-20 1976-06-08 Smithkline Corporation Device for making a culture of CO2 requiring organisms
US5073495A (en) * 1988-10-21 1991-12-17 Large Scale Biology Corporation Apparatus for isolating cloned vectors and cells having a recovery device
US6063038A (en) * 1999-04-09 2000-05-16 Clmp, Inc. Devices and methods for collecting fecal antigen specimens
WO2000061010A1 (en) * 1999-04-09 2000-10-19 Quest Diagnostics Investments Incorporated Devices and methods for collecting fecal antigen specimens

Also Published As

Publication number Publication date
IT1012701B (it) 1977-03-10
LU70147A1 (it) 1974-10-09
NL178264B (nl) 1985-09-16
FR2230721A1 (it) 1974-12-20
FR2230721B1 (it) 1976-12-24
JPS5721977B2 (it) 1982-05-11
JPS5018683A (it) 1975-02-27
CA1030439A (en) 1978-05-02
DE2424384A1 (de) 1974-12-19
DE2424384C2 (de) 1982-11-11
BE815419A (fr) 1974-11-22
ES426509A1 (es) 1976-07-01
DE7417496U (de) 1984-02-16
CH599346A5 (it) 1978-05-31
AU6910274A (en) 1975-11-20
GB1421683A (en) 1976-01-21
NL7406751A (it) 1974-11-26
NL178264C (nl) 1986-02-17

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Date Code Title Description
AS Assignment

Owner name: SMITHKLINE BECKMAN CORPORATION

Free format text: CHANGE OF NAME;ASSIGNOR:SMITHKLINE CORPORATION;REEL/FRAME:004080/0769

Effective date: 19820304

Owner name: SMITHKLINE BECKMAN CORPORATION, PENNSYLVANIA

Free format text: CHANGE OF NAME;ASSIGNOR:SMITHKLINE CORPORATION;REEL/FRAME:004080/0769

Effective date: 19820304