US3799162A - Method of producing collagenic material for the closure of lesions - Google Patents

Method of producing collagenic material for the closure of lesions Download PDF

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Publication number
US3799162A
US3799162A US00280736A US28073672A US3799162A US 3799162 A US3799162 A US 3799162A US 00280736 A US00280736 A US 00280736A US 28073672 A US28073672 A US 28073672A US 3799162 A US3799162 A US 3799162A
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Prior art keywords
histamine
incision
treated
saline
rats
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US00280736A
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Sierra C Romero
J Tanner
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ROMERO-SIERRA CEAR A BOX 32 BATH ONTARIO CANADA KOH 1GO
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Canadian Patents and Development Ltd
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Priority to US00280736A priority Critical patent/US3799162A/en
Priority to CA175,254A priority patent/CA992616A/en
Priority to DE19732337605 priority patent/DE2337605A1/de
Priority to JP9079873A priority patent/JPS533199B2/ja
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Assigned to ROMERO-SIERRA CEAR A. BOX 32, BATH ONTARIO CANADA KOH 1GO reassignment ROMERO-SIERRA CEAR A. BOX 32, BATH ONTARIO CANADA KOH 1GO ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: CANADIAN PATENTS AND DEVELOPMENT LIMITED
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B17/00—Surgical instruments, devices or methods
    • A61B17/04—Surgical instruments, devices or methods for suturing wounds; Holders or packages for needles or suture materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/40—Applying electric fields by inductive or capacitive coupling ; Applying radio-frequency signals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N5/00—Radiation therapy

Definitions

  • ABSTRACT Primary ExaminerRichard A. Gaudet Assistant Examiner-Henry J Recla Attorney, Agent, or Firm-Francis W. Lemon [5 7] ABSTRACT The production of collagenic material for the closure of lesions in enhanced, and thus the healing of a lesion accelerated by applying histamine to the lesion and then radiating the tissue cells bounding the lesion with electromagnetic radiation.
  • the electromagnetic radiation being in the non-ionizing-range of frequency, and having an intensity less than one milliwatt.
  • This invention relates to a method of producing collagenic material for the closure of lesions.
  • a method of producing collagenic material for the closure of lesions comprising applying histamine to the lesion, and radiating tissue cells bounding the lesion with electromagnetic radiation, the electromagnetic radiation being in the non ionizing range of frequency, and having an intensity less than one milliwatt, so that the reproduction of collagenic material by the tissue cells is enhanced.
  • FIG. I is a diagrammatic view of an adult rat having a lesion coated with histamine and subjected to electromagneticradiation to produce collagenic material.
  • FIG. 2 is a magnified transverse view (450X) of the incision area of a control rat wherein the incision has been treated with saline only,
  • FIG. 3 is a magnified transverse view (45OX) of a control rat wherein the incision has been treated with saline and radiated with an electromagnetic field,
  • FIG. 4 is a magnified (450X) transverse view of the incision area of a rat treated with histamine only
  • FIG. 5 is a magnified 450x transverse view of the incision area of a rat treated with histamine and radiated with an electromagnetic field.
  • FIG. 1 there is shown an adult rat 1 which has been anesthetized with ether and Nembutal. The back of the rat has been shaved and a horizontal lesion or incision 2 made with scissors (not shown) in the areajust below the scapulae.
  • a rod electrode 4 is connected by a cable 6 to an electromagnetic energy generator 8 for producing a 27 MHz field of electromagnetic radiation.
  • the generator 8 is connected by a cable 10 to a power source 12.
  • rats 1 after being anesthetized were given the incision 2, and histamine was applied to the incisions 2.
  • the incisions 2 were then radiated with electromagnetic energy from the electrode 4 with the electrode 4 placed lengthwise along the incision 2 and held proximal thereto.
  • THE TESTS Twelve adult rats 1 weighing from 200 to 300 grams were used in this study. The rats were divided into four groups: control rats treated only with saline applied 10- cally to the incision 2; test rats 1 treated with two drops of saline to the incision 2 and 15 minutes exposure to a VHF (27MHz) field with the electrode 4 held 0 5 mm from the area; control rats 1 treated with two drops of histamine diphosphate (1.0 mg per ml); applied to the incision 2 and test rats 1 treated with two drops of histamine disphosphate (1.0 mg. per ml) applied to the incision 2 and 15 minutes exposure to a VHF (27MH2) field with the electrode held 0-5 mm above the incision 2.
  • control rats 1 treated only with saline applied 10- cally to the incision 2 test rats 1 treated with two drops of saline to the incision 2 and 15 minutes exposure to a VHF (27MHz) field with the electrode 4 held 0 5 mm from the area
  • test and control rats 1 After anesthetizing the rats with ether and Nembutal, the backs of test and control rats 1 were shaved and a l to 1.5 cm horizontal incision 2 was made with scissors in the area just below the scapulae. Any blood that appeared in the wound area near the incision 2 was removed with gauze and the point of a 20 guage needle was used to adjust the edges of the incision 2 and to appose them. After application of the saline or histamine to the incision 2, the edges of both test and control wounds near the incisions 2 were carefully apposed and held tightly together. For the test rats 1, the electrode was held at a distance of 0 5 mm above the incision .2. After 15 minutes both control and test rats 1 were returned to individual cages for 24 hours. An attempt was made to perform each operation-in as uniform a way as possible and to limit each operational proce dure to a maximum of 25 minutes.
  • FIG. 2 there is shown a magnified (450X) transverse view of the incision area of a control rat 1 receiving only saline (control group 1 with saline).
  • control group 1 with saline control group 1 with saline.
  • FIG. 3 there is shown a magnified (450X) transverse view of the incision area showing the predominance of mononuclear cells in the infiltrate and the density of the fibrin base for test group 1 treated with saline and radiated with an electromagnetic field.
  • FIG. 4 there is shown a magnified (450X) transverse view of the incision area of a rat 1 of test group No. 2 treated with histamine and not radiated with an electromagnetic field.
  • the incision area contains approximately equal numbers of polymorphonuclear cells and mononuclear cells.
  • FIG. 5 there is shown a magnified (450X) transverse view of the incision area of a rat 1 of test group No. 2 treated with histamine and radiated with an electromagnetic field contains a large amount of fibrin and only a few scattered mononuclear cells.
  • histamine is essential for normal wound healing. Although its function in the healing process is not entirely understood, it is known that histamine causes dilatation of the capillaries in the body and an increase in the permeability of the fine vessels on the venous side of the capillary bed. It would not be possible to say by what exact mechanism histamine is acting in the present study to influencewound healing.
  • One specific advantage provided by the treatment with histamine according to the present invention was the increased manageability of the wound edges.
  • a method of producing collagenic material for the closure of lesions comprising applying histamine to the lesion, and radiating tissue cells bounding the lesion with electromagnetic radiation, the electromagnetic radiation being in the non-ionizing range of frequency, having an intensity less than one milliwatt so that the reproduction of collagenic material by the tissue cells is enhanced.
  • tissue cells are radiated with a 27 MHz electromagnetic radiation from an electrode held proximal to the tissue cells for 15 minutes.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Surgery (AREA)
  • Radiology & Medical Imaging (AREA)
  • Pathology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Molecular Biology (AREA)
  • Electrotherapy Devices (AREA)
  • Surgical Instruments (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Materials For Medical Uses (AREA)
US00280736A 1972-08-14 1972-08-14 Method of producing collagenic material for the closure of lesions Expired - Lifetime US3799162A (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US00280736A US3799162A (en) 1972-08-14 1972-08-14 Method of producing collagenic material for the closure of lesions
CA175,254A CA992616A (en) 1972-08-14 1973-06-29 Apparatus for producing collagenic material for the closure of lesions
DE19732337605 DE2337605A1 (de) 1972-08-14 1973-07-24 Verfahren und vorrichtung zur erzeugung von kollag aterial zum verschliessen von verletzungen
JP9079873A JPS533199B2 (enrdf_load_html_response) 1972-08-14 1973-08-13

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Application Number Priority Date Filing Date Title
US00280736A US3799162A (en) 1972-08-14 1972-08-14 Method of producing collagenic material for the closure of lesions
DE19732337605 DE2337605A1 (de) 1972-08-14 1973-07-24 Verfahren und vorrichtung zur erzeugung von kollag aterial zum verschliessen von verletzungen

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JP (1) JPS533199B2 (enrdf_load_html_response)
CA (1) CA992616A (enrdf_load_html_response)
DE (1) DE2337605A1 (enrdf_load_html_response)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4767401A (en) * 1975-04-22 1988-08-30 Maurice Seiderman Iontophoretic administration of ionizable or polar medicaments to a mammalian body
US5814094A (en) * 1996-03-28 1998-09-29 Becker; Robert O. Iontopheretic system for stimulation of tissue healing and regeneration
US20050244484A1 (en) * 1997-09-22 2005-11-03 Flick A B Multilayer conductive appliance having wound healing and analgesic properties
US20060264796A1 (en) * 1995-09-05 2006-11-23 Argentum Medical, Llc Medical device
US7214847B1 (en) 1997-09-22 2007-05-08 Argentum Medical, L.L.C. Multilayer conductive appliance having wound healing and analgesic properties
US7291762B2 (en) 1997-09-22 2007-11-06 Argentum International, Llc Multilayer conductive appliance having wound healing and analgesic properties
US8449514B2 (en) 1997-09-22 2013-05-28 Argentum Medical, Llc Conductive wound dressings and methods of use

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3557783A (en) * 1967-04-12 1971-01-26 Charles S Castner Method of killing microprobes with microbicidal compounds and ultraviolet light

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3557783A (en) * 1967-04-12 1971-01-26 Charles S Castner Method of killing microprobes with microbicidal compounds and ultraviolet light

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Annals of Surgery, Jan. 1963, Vol. 157, No. 1; Origin of Fibroblasts in Wound Healing: An Autoradiographic Study of Inhibition of Cellular Proliferation by Local X Irradiation, H. C. Grillo, June 5, 1962, pp. 453 67. *
Collagen Currents, Nov. 1960, Vol. 1, No. 5, Effects of Whole Body X Irradiation on Wound Healing, Surgeons Forum 10 840 4 (1960). *
Collagen Currents, Sept. 1960, Vol. 1, No. 3; The Effect of Histamine and a Histamine Releasing Agent Compound 48/80 on Wound Healing, Boyd & Smith, Journal of Pathology & Bacteria, 78 379 88 (1959); Biology Abstracts 35 32145, June 15, 1960. *
The Pharmacological Basis of Therapeutics, 3rd Ed. June 6, 1966, Histamines and Antihistamines, William W. Douglas. *

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4767401A (en) * 1975-04-22 1988-08-30 Maurice Seiderman Iontophoretic administration of ionizable or polar medicaments to a mammalian body
US8118791B2 (en) 1995-09-05 2012-02-21 Argentum Medical, Llc Medical device
US8801681B2 (en) 1995-09-05 2014-08-12 Argentum Medical, Llc Medical device
US20060264796A1 (en) * 1995-09-05 2006-11-23 Argentum Medical, Llc Medical device
US8293964B2 (en) 1995-09-05 2012-10-23 Argentum Medical, Llc Multilayer laminate wound dressing
US20070179522A1 (en) * 1995-09-05 2007-08-02 Argentum Medical, Llc Multilayer wound dressing
US8283513B2 (en) 1995-09-05 2012-10-09 Argentum Medical, Llc Multilayer wound dressing
US5814094A (en) * 1996-03-28 1998-09-29 Becker; Robert O. Iontopheretic system for stimulation of tissue healing and regeneration
US7214847B1 (en) 1997-09-22 2007-05-08 Argentum Medical, L.L.C. Multilayer conductive appliance having wound healing and analgesic properties
US7989674B2 (en) 1997-09-22 2011-08-02 Argentum Medical, Llc Multilayer conductive appliance having wound healing and analgesic properties
US8093444B2 (en) 1997-09-22 2012-01-10 Argentum Medical, Llc Multilayer conductive appliance having wound healing and analgesic properties
US20080033506A1 (en) * 1997-09-22 2008-02-07 Argentum International, Llc Multilayer Conductive Appliance Having Wound Healing and Analgesic Properties
US7291762B2 (en) 1997-09-22 2007-11-06 Argentum International, Llc Multilayer conductive appliance having wound healing and analgesic properties
US7230153B2 (en) 1997-09-22 2007-06-12 Argentum International, Llc Multilayer conductive appliance having wound healing and analgesic properties
US8449514B2 (en) 1997-09-22 2013-05-28 Argentum Medical, Llc Conductive wound dressings and methods of use
US8455710B2 (en) 1997-09-22 2013-06-04 Argentum Medical, Llc Conductive wound dressings and methods of use
US20050244484A1 (en) * 1997-09-22 2005-11-03 Flick A B Multilayer conductive appliance having wound healing and analgesic properties

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Publication number Publication date
JPS533199B2 (enrdf_load_html_response) 1978-02-03
JPS4952491A (enrdf_load_html_response) 1974-05-21
CA992616A (en) 1976-07-06
DE2337605A1 (de) 1975-02-20

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