Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K51/00—Preparations containing radioactive substances for use in therapy or testing in vivo
  • A61K51/12—Preparations containing radioactive substances for use in therapy or testing in vivo characterised by a special physical form, e.g. emulsion, microcapsules, liposomes, characterized by a special physical form, e.g. emulsions, dispersions, microcapsules
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K2123/00—Preparations for testing in vivo

Definitions

• the present invention relates to a method of producing a diagnostic preparation on the basis of an iron complex labelled with 99m Tc, with preparation is of importance for various scintigraphic examinations and especially for examinations of the brain and the kidneys.
• Technetium is in great request for use in medicine owing to its short half-life (6 hours) its u-ray energy (140 kev.) and the absence of emission of SI-rays. As a result, the radiation doses administered to the patients may be reduced, whilst the injected activity and hence the statistical accuracy of the counts is increased and the duration of the examination may be reduced.
• radioactive technetium The wide range of application of radioactive technetium is of great importance, because it enables the store of other radioactive elements to be restricted.
• radioactive technetium may simply be obtained at any desired instant by means of an elution process using, for example, the generator system described in French patent specification No. 1,518,130, in the form of sodium pertechnetate in an isotonic saline solution which may be mixed with the carrier required for the respective examination.
• the method according to the invention is characterized in that there is added to powdered ferrous ascorbate a solution of a technetium cmopund, after which if required, the resulting solution is made alkaline to a pH between 7 and 8, and finally the reaction mixture is agitated.
• the powdered ferro-ascorbate may be dissolved in an aqueous medium such, for example, as a physiological saline solution.
• the reaction mixture is agitated in the presence of air, until a violet color appears. This treatment takes a few minutes. The violet color corresponds to the formation of a technetium-labelled ion complex.
• the resulting product is ready for use and may be injected into the patient to be examined.
• the product is stable for several hours.
• the freeze-dried ferrous ascorbate has the further advantage that it remains stable for a prolonged period of time.
• the technetium compound used in the method according 'to the invention preferably is sodium pertechnetate.
• the adjustment of the pH of the reaction mixture preferably is eifected by means of a trihydroxymethylaminomethane solution (sometimes referred to as Tris or THAM) which previously has been buffered to a pH of about 8 by the addition of hydrochloric acid.
• Tris or THAM trihydroxymethylaminomethane solution
• the starting product is a powdered mixture of ferrous ascorbate and a basic substance.
• the amount of basic substance is such that after the solution of the technetium compound has been added the reaction mixture has a pH of from 7 to 8.
• the basic substance preferably has a buffer capacity at pH values between 7 and 8.
• the adjustment of the pH after the addition of the technetium compound is not necessary.
• the method according to the invention eliminates the afore-described disadvantages.
• a further advantage of the method according to the invention consists in an increase in the labelling efliciency.
• the method according to the invention results in a labelling efiiciency of from to 97%.
• the invention also relates to a kit to be marketed as a unit which is suitable for use in the method according to the invention, which kit comprises at least one bottle of sterilized powdered ferrous ascorbate and at least one bottle of sterilized alkaline substance.
• bottles other hermetically sealed containers, such as ampoules, may be used in the kit according to the invention.
• the required radioactive solution must be prepared in situ by means of elution of radioactive technetium generator.
• the invention further relates to a new preparation for use in the method according to the invention, which preparation is characterized in that it comprises a pow- EXAMPLE I
• a pow- EXAMPLE I To 33 mg. of ferrous ascorbate there is added 2 ml. of a solution of TcO Na; to the resulting solution there is added 0.8 ml. of a solution of Tris (0.2 M) and the mixture is agitated in the presence of air, until a violet color is produced.
• the labelling efliciency which has been determined by means of anion resins and by thinlayer chromatography, is 95%.
• a method of producing a diagnostic preparation on the basis of an iron complex labelled with 99m Tc comprising adding to powdered ferrous ascorbate a solution of a technetium compound, adjusting the solution to a pH between 7 and 8 if necessary and agitating the reaction mixture in the presence of air until a violet color is produced.

Landscapes

• Health & Medical Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Pharmacology & Pharmacy (AREA)
• Physics & Mathematics (AREA)
• Medicinal Chemistry (AREA)
• Optics & Photonics (AREA)
• Dispersion Chemistry (AREA)
• Epidemiology (AREA)
• Life Sciences & Earth Sciences (AREA)
• Animal Behavior & Ethology (AREA)
• General Health & Medical Sciences (AREA)
• Public Health (AREA)
• Veterinary Medicine (AREA)
• Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Applications Claiming Priority (1)

Publications (1)

Family

ID=9055977

Family Applications (1)

Country Status (10)

Cited By (3)

Families Citing this family (2)

• 1970
  • 1970-05-22 FR FR7018792A patent/FR2088149B1/fr not_active Expired
• 1971
  • 1971-05-18 NL NL7106774A patent/NL7106774A/xx not_active Application Discontinuation
  • 1971-05-19 DE DE19712124751 patent/DE2124751A1/de active Pending
  • 1971-05-19 IT IT24779/71A patent/IT1050136B/it active
  • 1971-05-19 GB GB1577171*[A patent/GB1320626A/en not_active Expired
  • 1971-05-19 CH CH738871A patent/CH554667A/xx not_active IP Right Cessation
  • 1971-05-19 DK DK244271AA patent/DK128555B/da unknown
  • 1971-05-21 CA CA113,598A patent/CA945067A/en not_active Expired
  • 1971-05-21 BE BE767503A patent/BE767503A/nl unknown
  • 1971-05-24 US US00146543A patent/US3787565A/en not_active Expired - Lifetime

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