US3785549A - Centrifuge chuck for disposable, snap-in centrifuge rotor - Google Patents

Centrifuge chuck for disposable, snap-in centrifuge rotor Download PDF

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Publication number
US3785549A
US3785549A US00276677A US3785549DA US3785549A US 3785549 A US3785549 A US 3785549A US 00276677 A US00276677 A US 00276677A US 3785549D A US3785549D A US 3785549DA US 3785549 A US3785549 A US 3785549A
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United States
Prior art keywords
rotor
chuck
gripping
centrifuge
section
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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US00276677A
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English (en)
Inventor
A Latham
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Haemonetics Corp
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Haemonetics Corp
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Publication date
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Publication of US3785549A publication Critical patent/US3785549A/en
Assigned to AMERICAN HOSPITAL SPPLY CORPORATION, A CORP. OF IL. reassignment AMERICAN HOSPITAL SPPLY CORPORATION, A CORP. OF IL. ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: HAEMONETICS CORPORATION, A CORP. OF DE.
Assigned to FLEET CREDIT CORPORATION, FLEET NATIONAL BANK, A NATIONAL BANKING ASSOCIATION reassignment FLEET CREDIT CORPORATION SECURITY INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: LATHAM LABS, INC., A CORP. OF MA.
Assigned to HAEMONETICS CORPORATION reassignment HAEMONETICS CORPORATION CHANGE OF NAME (SEE DOCUMENT FOR DETAILS). EFFECTIVE DATE: DECEMBER 12, 1985 Assignors: LATHAM LABS, INC. (CHANGED TO)
Assigned to LATHAM LABS, INC. reassignment LATHAM LABS, INC. ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: AMERICAN HOSPITAL SUPPLY CORPORATION, A CORP OF IL.
Assigned to HAEMONETICS CORPORATION, A MASSACHUSETTS CORP. reassignment HAEMONETICS CORPORATION, A MASSACHUSETTS CORP. ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: FLEET NATIONAL BANK
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B7/00—Elements of centrifuges
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • B04B2005/0464—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation with hollow or massive core in centrifuge bowl

Definitions

  • This invention relates to centrifuges and more partic-' I ularly to a unique combination of a chuck and a snap-in disposable centrifuge rotor for attachment thereto.
  • the disposable rotor of this, invention is of an upwardly tapering, bell-shaped configuration which is not adapted for manual gripping to lift it out of a standard O-ring form of chuck.
  • a mechanical ejection system chuck such as described in U.S. Pat. No.3,581.98l, provides a technically acceptable way of extracting the bowl from the chuck.
  • this type of chuck is too expensive.
  • the chuck is formed of an upper clamp ring and a lower chuck body on which the rotor fits for automatic axial and perpendicular alignment.
  • the upper clamp ring and lower chuck body are configuredto define between them a groove adapted to hold an elastomeric gripping member, and they may beformed as one integral part or as two separate components adapted to be locked together by screws.
  • the base of the rotor is designed to provide an upwardly inclined shoulder over which the elastomeric gripping member fits in gripping engagement.
  • the chuck is formed of two lockable components when the screws which lock the chuck members are turned about one full turn to loosen the chuck members the rotor may be snapped into place in the chuck.
  • the rotor is snapped into place, the screws are tightened and the centrifuge is in operational condition. Removal of the rotor is accomplished with similar ease.
  • the chuck is formed as a single component the rotor is installed with a firm, downward force normally applied with both hands and may be removed with the aid of a simple tool.
  • the invention accordingly comprises the features of construction, combination of elements, and arrange ment of parts which will be exemplified in the construc- 2 tion hereinafter set forth, and the scope of the invention will be indicated in the claims.
  • FIG. 1 is a vertical cross section of the chuck and rotor of an embodiment of this invention in which the chuck is formed of two separate components;
  • FIG. 2 is a bottom view of the chuck of FIG. 1 showing the position of the locking screws
  • FIG. 3 is an enlarged detailed cross section showing the configurations of the chuck of FIG. 1, the centrifuge rotor base, the elastomeric gripping ring and a locking screw in the unlocked position;
  • FIG. 4 is an enlarged detailed cross section as in FIG.
  • FIG. 5 is an enlarged detailed cross section of one portion of the chuck and rotor of an embodiment in which the chuck is formed as a single component and the gripping member is of a unique configuration.
  • FIG. 1 the chuck and gripping member are shown in cross section and the rotor in partial crosssection. Except for the design of the external configuration of the base of the rotor which is critical to this invention, the rotor shown is similar in internal construction and operation to that described in U.S. Pat. No. 3,145,713 (see particularly FIG. 6 of that patent). For this reason, no detailed description of the internal design of the rotor is required, only the basic flow pattern being illustrated.
  • the rotor 10 is mounted'in chuck 11 through elastomeric gripping member 12.
  • the chuck in the embodiment of FIG. 1 is formed of an upper clamp means formed as ring 13 anda lower chuck body 14, the latter being adapted for attachment to a rotatable spindle 15 such as the temperature-controlled spindle described in U.S. Pat. No. 3,604,769.
  • the upper and lower chuck members are locked by at least'three locking members 16 (See also FIG. 2
  • the rotor 10 which may be a one-use disposable rotor, is designed to be attached through a stationary external connection 20 to a source of liquid (e.g., blood) to be centrifuged through inlet tube 21, and to a storage container for the centrifugate (e.g., blood plasma) through discharge tube 22.
  • the stationary inlet tube 20 is in fluid communication with a stationary feed tube 23 extending. into the rotor.
  • Rotary. seals such as described in U.S. Pat. Nos. 3,409,213 and 3,565,330 provide the necessary contamination-free sealing between the stationary-components and the rotating rotor 10.
  • a baffle system such as that shown in FIG. 1 to be made up of baffle plates 24 and 25 direct the liquid flow and permit the centrifuged solids (e.g., blood red cells) to be collected in the centrifuge rotor.
  • the rotor housing is formed as a tapered bell-shaped section 30 and a bottom member 31 which is joined to the bell-shaped section to form, with a rotary seal (not shown) a fluid-tight enclosure within the housing.
  • the bottom member is formed from its center outwardly to have a central circular alignment surface 32, an upwardly inclined surface 33 forming a major portion of the bottom member and a flat peripheral surface 34.
  • the outer lower periphery of the base of the bell-shaped section is configured to define an inclined shoulder 35 the angle of inclination Qof whichapproximates 45. that is it is 45 1
  • the shoulder 35 is formed between a lower wall section, consisting of a slightly inclined wall 36 and a vertical wall 37, and upper vertical wall section 38.
  • the angle defined between wall 36 and the vertical ranges between 15 and 30 and the height of vertical wall 37, which represents the maximum diameter of the rotor base will normally be about 0.03 inch.
  • wall 36 may also be vertical, a preferred construction is to provide a lead" on the rim of the base to make insertion of the bowl easier.
  • the upper surface of chuck body 14 is configured, from its center outwardly, to have a shallow central well 40 sized to correspond in diameter to the diameter of the central circular alignment surface 32 of the rotor, an upwardly inclined surface 41 corresponding to, but spaced from, the upwardly inclined surface 33 of the rotor and a flat annular surface 42 corresponding to the flat peripheral surface 34 of the rotor.
  • the engagement of the flat peripheral surfaces 34 and 42 and of the central circular alignment surface 32 of the rotor within the shallow central well 40 automatically provides both perpendicular and axial alignment of the rotor in the chuck body.
  • the chuck is mounted on spindle 15 through collar 43 by suitable means such as by use of an adhesive along with a close cylindrical fit.
  • the lower chuck body 14 has an outer flat peripheral surface 44 somewhat lower than the flat annular surface 42.
  • An upwardly extending annular ring 45 joins flat annular surfaces 42 and 44.
  • the upper surface 46 of ring 45 serves as one side of groove 47 which is adapted to contain elastomeric gripping member in the form of an O-ring 12.
  • the inner surface 48 of ring 45 is of a diameter which permits engagement with the vertical wall 37 of the lower wall section of the rotor base with preferably about an 0.010 inch clearance. This clearance can range between about 0.008 and 0.015 inch.
  • the lower edge of gripping shoulder 35 of the rotor base is between about 0.1 and 0.2 inch above the perpendiculant register, i.e., annular surface 42, for a rotor which has a diameter ranging between about 4.0 and 5.5 inches in diameter measured at the vertical wall 37 of the lower section of the rotor base.
  • the ratio of rotor base edge height, as measured along the bottom edge of shoulder 35, above the perpendiculant register to the outside diameter of the rotor base may range between about 0.1/5.5 to 0.2/4.0 or about 0.02 to 0.05.
  • Another relationship which may be used to define the configuration of the rotor base and the gripping means is the ratio of rotor base edge height (distance from surface 42 to the bottom edge of wall 37) to shoulder edge height (distance from surface 42 to the bottom edge of shoulder 35). This ratio should be between about 0.2 and 0.6.
  • the upper chuck clamp ring 13 will be seen in FIGS. 3 and 4 to have an internal surface which is machined to define a lower vertical wall section 51 and an upper vertical wall section 52 of small diameter. These wall sections are joined by shoulder 53.
  • the lower vertical wall section 51 is aligned with the outer surface of ring 45 for engagement herewith.
  • the upper portion of wall member groove 47 the depth of which is controllable to alter the cross sectional configuration of the O-ring to cause it to loosen or grip the rotor as seen in FIGS. 3 and 4, respectively.
  • Each of the chuck locking means 16 are conveniently formed of a clamp screw 55 which is internally threaded to engage the threads of a button-headed screw 56.
  • the locking means are loosened by turning clamp screw 55 by about one turn to place the chuck clamp ring and chuck body in the relative positions shown in FIG. 3.
  • the rotor is gripped manually around its top and is then thrust firmly downwardly to become engaged in the position shown in FIG. 3. It will be seen that all that is involved is the insertion of the gripping shoulder 35 under a small portion of O-ring l2. Rotor alingment in the chuck is automatically achieved.
  • the locking means are tightened by turning screws 55 by about one full turn.
  • the downward movement of clamp ring 13 forces the O-ring to engage and tightly grip the rotor shoulder 35.
  • the rotor is thus firmly held in the chuck when the chuck is rotated.
  • the chuck clamp ring is raised by turning screws 55 and the rotor is removed by subjecting it to a tipping action.
  • FIG. 5 illustrates in fragmented cross section a second embodiment of the chuck and gripping means wherein the lower chuck body means and the upper chuck ring means are formed as one integral component, thus eliminating the need for any chuck locking means such as the clamp screw 55 and buttonheaded screw 56 of FIGS. 3 and 4.
  • like reference numerals are used to refer to like components in FIGS. 3 and 4.
  • the chuck 11 of the apparatus of FIG. 5 is formed of a single metal piece 60 which provides the required lower chuck body 61 and upper chuck ring 62. It is, of course, within the scope of this invention to form these elements as separate components and fasten them together by some appropriate means such as screws. However, this will normally not be the preferred construction.
  • the groove 63 wherein the elastomeric gripping ring 64 is held, is machined into the internal surface 65 of chuck ring 62.
  • the gripping member 64 is an annular elastomeric member cut to set in groove 63 and to make a tight fit with lower vertical wall 37 and shoulder 35 of the rotor bottom.
  • the cross section of gripping member 64 may be described as rectangular with a triangular extension 66 integral with the inner top portion, the lower exposed surface 67 of the triangle being adapted to make a tight fit with shoulder 35 of the rotor base.
  • FIG. 5 A comparison with gripping ring 12 of FIGS. 3 and 4, will show that the uniquely configured locking member 64 of FIG. 5 serves the identical purpose of gripping ring 12 and in the same manner.
  • the apparatus of FIG. 5 has the advantage of the operators being able to insert and withdraw the rotor without having to screw and unscrew any locking means. Insertion of the rotor is accomplished by forcing it into locking position (FIG. 5) with a firm force applied directly downwardly. Removal of the rotor is accomplished by using the tool 70 which is in effect a rubber coated lever 71 attached to a handle 72. The lever 71 is used to apply force to centrifuge housing 30, using the internal wall of the protective housing 73, which is placed around the centrifuge, as s rigid support against which lever 71 is rested. A slight angular movement of handle 72 of the departing from the scope of the invention, it is intended that all matter contained in the above description or shown in the accompanying drawings shall be interpreted as illustrative and not in a limiting sense.
  • a centrifuge in which a rotor is held for highspeed rotation in a chuck mounted on a rotatable spindle, the combination of a rotor and a chuck adapted for quick engagement with said rotor through gripping means, said chuck comprising in combination a. lower chuck body means configured on its upper surface to comprise, from its center outwardly, a
  • upper chuck ring means providing an angled internal surface
  • said rotor comprising d. a bottom member configured on its surface to comprise, from its center outwardly,Ia central circular alignment surface engaging said wall of said central shallow well of said chuck, an upwardly inclined surface essentially corresponding in area to said inclined surface of said lower chuck body means and spaced therefrom, and a peripheral flat annular surface engaging said first flat annular surface of said lower chuck body means, and
  • rotor housing means joined to said bottom member and forming therewith a rotor base configured from the bottom up to have an outwardly inclined section, a lower vertical gripping section representing the maximum diameter of said rotor base and just clearing said internal surface of said upwardly extending wall of said lower chuck body means, an inwardly inclined gripping shoulder and an upper vertical section; forcing f. and gripping means comprising an elastomeric gripping ring held within said groove in said chuck in combination with locking means forcing said lower v'ertical gripping section and said inwardly inclined gripping shoulder of said rotor base into engagement with said gripping ring thereby to lock said rotor to said chuck through said gripping means.
  • a centrifuge in accordance with claim 1 wherein said lower chuck body means is separate from said upper chuck ring means and includes a flat peripheral surface which surrounds said upwardly extending wall and is lower than said first flat annular surface; wherein said angled internal surface of said upper chuck ring means is defined by a lower vertical wall section, and an upper vertical wall section joined thereto through a shoulder, whereby a portion of said lower vertical wall section engages an'external surface of said upwardly extending wall and with said shoulder forms said groove, and wherein said locking means comprises screw means to force said upper chuck ring means toward said lower chuck body means thereby'to force said elastomeric gripping ring and said rotor base into engagement.

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  • Centrifugal Separators (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
US00276677A 1972-07-31 1972-07-31 Centrifuge chuck for disposable, snap-in centrifuge rotor Expired - Lifetime US3785549A (en)

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US27667772A 1972-07-31 1972-07-31

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US (1) US3785549A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
JP (1) JPS5713345B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CA (1) CA979869A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
DE (1) DE2328346C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
FR (1) FR2194488B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
GB (1) GB1436003A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IT (1) IT986569B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

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DE2745041A1 (de) * 1976-10-06 1978-04-13 Haemonetics Corp Plasmaphorese-vorrichtung
US4684361A (en) * 1985-10-11 1987-08-04 Cardiovascular Systems, Inc. Centrifuge
US4692136A (en) * 1985-10-11 1987-09-08 Cardiovascular Systems Inc. Centrifuge
FR2595268A1 (fr) * 1986-03-10 1987-09-11 Cardivascular Systems Montage tournant pour fixer en position un bol centrifuge pendant la rotation d'une centrifugeuse
US4752284A (en) * 1986-12-05 1988-06-21 Biscar Jean P Artificial gravity intracellular molecular extraction
US4767396A (en) * 1987-03-03 1988-08-30 Haemonetics Corporation Method and apparatus for processing biological fluids
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EP0278381A3 (en) * 1987-02-10 1989-01-25 Dideco S.P.A. Chuck device for holding a cell for the centrifugation of blood, and the like
WO1989001827A1 (en) * 1987-09-04 1989-03-09 Haemonetics Corporation Portable centrifuge apparatus
AU592598B2 (en) * 1985-10-11 1990-01-18 Cobe Laboratories Inc. Centrifuge
US5062826A (en) * 1989-07-14 1991-11-05 Marco Mantovani Device for locking a blood centrifugation cell on a chuck
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US5344381A (en) * 1992-07-10 1994-09-06 Cabrera Y Lopez Caram Luis F Equipment for the elimination of light particles, inks and air from a fiber suspension for the manufacture of paper
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US5658231A (en) * 1995-09-21 1997-08-19 Haemonetics Corporation Mechanism for securing a separation bowl to a mechanical chuck
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US20020077241A1 (en) * 1999-09-03 2002-06-20 Baxter International Inc. Blood processing systems and methods with quick attachment of a blood separation chamber to a centrifuge rotor
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DE10311168B4 (de) * 2003-03-12 2006-05-11 Westfalia Separator Ag Schleudertrommel für einen Separator
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DE2328346A1 (de) 1974-02-14
JPS4958463A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 1974-06-06
FR2194488B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 1978-06-30
FR2194488A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 1974-03-01
CA979869A (en) 1975-12-16
GB1436003A (en) 1976-05-19
IT986569B (it) 1975-01-30
JPS5713345B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 1982-03-16
DE2328346C2 (de) 1985-06-13

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