US3754654A - Device for applying a liquid along a line on a support - Google Patents

Device for applying a liquid along a line on a support Download PDF

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Publication number
US3754654A
US3754654A US00131904A US3754654DA US3754654A US 3754654 A US3754654 A US 3754654A US 00131904 A US00131904 A US 00131904A US 3754654D A US3754654D A US 3754654DA US 3754654 A US3754654 A US 3754654A
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US
United States
Prior art keywords
pipette
shaft
carriage
movement
path
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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US00131904A
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English (en)
Inventor
E Nybom
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INSTRUMENTA AB
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INSTRUMENTA AB
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Publication date
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
    • G01N30/91—Application of the sample

Definitions

  • the pipette is mounted pivotally in order that a capillary liquid application prong thereon can be maintained in contact with the surface of the plate when moving in one direc-' tion while it is raised from the surface when the pipette is moved in the opposite direction.
  • the present invention relates to a device for linear application of a liquid on a carrier means such as a plate intended to be used in connection with a chromatographic investigation, and comprising a dose means in the form of a pipette or the like for the liquid, a support for said dose means, and means to move said support in parallel with the line to be coated.
  • the device according to the invention is characterized in that said support is pivotally mounted in such a manner that said pipette while moving in one direction is maintained in contact with the carrier means but is maintained out of contact while moving in the opposite direction.
  • the dose means comprises a pipette which consists of a cylindrical receptacle and a capillary prong preferably angularly disposed in relation to said receptacle.
  • the support is mounted so as to maintain the capillary prong directed obliquely downwards to the carrier means and backwards in relation to the motion direction while moving in contact with the carrier means and to maintain the capillary prong I directed obliquely upwards and forwards in relation to the motion direction while moving in the opposite direction.
  • the device according to the invention By means of the device according to the invention it is possible to move a pipette backward and forward over a carrier means while applying liquid in the one direction and with intermediate drying while the pipette is moving back to the initial position. This may be accomplished by means of an motor'driven motion and by application of a uniform pressure on the carrier means. By repeating said motion it is possible to enrich large amounts of dry substance along a narrow start line for subsequent chromatographic processing. If desired the device according to the invention may be fully automated in order to considerably facilitate the operation. 7
  • FIG. Ia shows a portion of the device according to line on a carrier means.
  • Said carrier means for instance may be a plate I, which is shown in FIG. 1 by dashed lines.
  • said plate therefore consists of a glass tile serving as carrier means and being provided with a thin chromatographic layer of, for instance, silica gel.
  • the device is provided with a pipette which also is shown by dashed lines and generally indicated by 2.
  • the pipette comprises a cylindrical receptacle portion 3 and a capillary prong portion 4.
  • the pipette is mounted in a support 5.
  • the support is pivoted on a shaft 6. By means of a set screw 7 the angular position of the support in relation to the shaft 6 is adjustable.
  • the shaft is journalled inbearings 8 in a carriage 9.
  • the carriage 9 by means of a belt 10 is movably mounted on bars 11 extending between a motor housing 12 and an operating housing 13 (FIGS. la and lb).
  • the support 5 is maintained in the shown position so that the capillary prong 4 is in contact with the plate 1.
  • an operating arm 14 engages an actuating member in the form of a projection or stop 15.
  • the projection 15 is secured to an angel plate 16 which in turn is secured to a bottom plate 17.
  • a second portion of said angel plate l6. is indicated by 18 and serves to actuate a microswitch 19.
  • the microswitch 19 by means of suitable electrical controls causes reversal of the motion direction of the carriage.
  • a corresponding microswitch 20 is mounted at the opposite side of the carriage in order to reverse the motion direction at the opposite end position of the carriage.
  • the operating arm 14 extends on both sides of the shaft 6.
  • the upper portion of the arm 14 is provided with a pin 23 which magnetically contacts a magnet 24.
  • the magnet 24 is slidable in a channel the cross section of which best appears from FIG/4.
  • the magnet 24 accompanies the pin 23.
  • the magnet comes into contact with a member 45 stationarily mounted in the motor housing, and said member will also be magnetically contacted by the magnet.
  • the microswitch 20 comes into contact with a stop 26 through which the motion directionis reversed.
  • the metal member 45 holds the magnet 24in the end position. In this way the operating arm 14 and the shaft 6 are rotated back to the position shown in FIG. 1 and the content of the pipette will again start to flow out.
  • the plate I is in contact with a guide rule 27 so that the pipette all the time coats the same line on the plate.
  • the operating housing is provided with various control means for the speed to the left, i. e., the return speed, and the speed to the right, i.e., the coating speed.
  • the hand wheels of said control means are indicated by 28 and 29. By this means it is possible to change partly the coating speed in relation to the viscosity of the liquid and partly the drying period between the coatings.
  • conventional control means (not shown) are used which actuate the motor 30.
  • the position of the capillary prong 4 in relation to the plate 1 is adjustable not only by means of the set screw 7 but also by means of a hand wheel 31 (FIG. 1).
  • the hand wheel 31 is mounted on a shaft 32 (FIG. 3) having a prolongation tip 33 (FIG. 2) which rests on one of the guide bars 11. Turning of the shaft 32 which is screwed into a bearing 34 will change the height of the carriage in relation to said guide bar 11. By this means it is therefore possible to raise or lower the prong 4.
  • FIG. 3 At the bottom to the left in FIG. 3 a locking device 35 is shown by means of which a pin 36 stationary mounted on the shaft 6 may be locked. Said locking device is used only when it is desired by means of the set screw 7 to adjust the position of the support in relation to the shaft 6.
  • 37 reference numeral indicates a hand wheel secured to a shaft 38 by means of which the locking device 35 is actuated.
  • a device for applying a liquid linearly onto a carrier means comprising a carriage, means for moving said carriage reciprocally and for supporting said carriage over and in parallel relation to the carrier means, a pipette means including a cylindrical receptacle and a capillary prong extending therefrom at an angle to the axis thereof for applying a dose of the liquid linearly onto the carrier means, a support for said pipette means mounted on said carriage for pivotal movement about an axis transverse to the path of movement of said carriage, and means at opposed ends of the path of movement of said carriage for reciprocally pivoting said support, whereby said pivotable support for said pipette means maintains said capillary prong obliquely downwards onto the carrier means at an acute angle thereto and backwards to the direction of movement over said carrier means while linearly applying liquid thereonto and maintains said capillary prong obliquely upwards away from the carrier means when the direction of movement is opposite to the first mentioned direction of movement to prevent flow of the liquid onto the carrier means
  • said support for said pipette means comprises a shaft rotatably mounted on said carriage and wherein said means for pivoting said support comprises separate means for rotating said shaft in opposite directions at each end of the path of reciprocal movement so as to raise said capillary prong away from the carrier means at one end of the path and to lower said prong onto the carrier means at the other end of the path.
  • one means for rotating said shaft to lower said pipette means comprises a first operating arm extending radially from said shaft, a magnet movably mounted on said carriage and in magnetic contact with said first operating arm, a fixed metal contact at one end of the path of movement, said magnet and said metal contact being attracted to each other at the one end of the path whereby upon reversal of movement of said carriage at the one end of the path, said metal contact will restrain said magnet and operating arm for sufficient time to cause said operating arm to rotate said shaft and thus lower said pipette means onto the carrier means.
  • a device as claimed in claim 3 wherein the other means for rotating said shaft to raise said pipette means comprises a second operating arm extending radially from said shaft, a fixed projection at the other end of the path of movement, whereby when said second operating arm strikes said projection said operating arm rotates said shaft to raise said pipette means away from the carrier means and also rotates the first operating arm to move said movable magnet to its initial position.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Coating Apparatus (AREA)
US00131904A 1970-04-13 1971-04-07 Device for applying a liquid along a line on a support Expired - Lifetime US3754654A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE04960/70A SE337126B (fr) 1970-04-13 1970-04-13

Publications (1)

Publication Number Publication Date
US3754654A true US3754654A (en) 1973-08-28

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Family Applications (1)

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US00131904A Expired - Lifetime US3754654A (en) 1970-04-13 1971-04-07 Device for applying a liquid along a line on a support

Country Status (7)

Country Link
US (1) US3754654A (fr)
CA (1) CA933746A (fr)
CH (1) CH537760A (fr)
DE (1) DE2116727A1 (fr)
FR (1) FR2089383A5 (fr)
GB (1) GB1341639A (fr)
SE (1) SE337126B (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0205106A2 (fr) * 1985-06-10 1986-12-17 Shandon Scientific Limited Centrifugeuse
US4740298A (en) * 1986-09-08 1988-04-26 Sepragen Corporation Chromatography column/moving belt interface
WO2003059515A2 (fr) * 2002-01-18 2003-07-24 Evotec Oai Ag Dispositif de transport de fluides et procede d'alimentation et d'evacuation de fluides

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1032096A (en) * 1964-04-16 1966-06-08 Desaga Labor Technik G M B H Apparatus for applying a mixture of substances in solution to a thin sorbent layer for separation chromatography
US3635345A (en) * 1969-03-13 1972-01-18 Jerome A Rodder Chromatography

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1032096A (en) * 1964-04-16 1966-06-08 Desaga Labor Technik G M B H Apparatus for applying a mixture of substances in solution to a thin sorbent layer for separation chromatography
US3635345A (en) * 1969-03-13 1972-01-18 Jerome A Rodder Chromatography

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0205106A2 (fr) * 1985-06-10 1986-12-17 Shandon Scientific Limited Centrifugeuse
EP0205106A3 (en) * 1985-06-10 1988-05-04 Shandon Southern Products Limited Centrifugation
US4740298A (en) * 1986-09-08 1988-04-26 Sepragen Corporation Chromatography column/moving belt interface
WO2003059515A2 (fr) * 2002-01-18 2003-07-24 Evotec Oai Ag Dispositif de transport de fluides et procede d'alimentation et d'evacuation de fluides
WO2003059515A3 (fr) * 2002-01-18 2004-01-15 Evotec Ag Dispositif de transport de fluides et procede d'alimentation et d'evacuation de fluides

Also Published As

Publication number Publication date
DE2116727A1 (de) 1971-10-28
CA933746A (en) 1973-09-18
GB1341639A (en) 1973-12-25
CH537760A (de) 1973-06-15
SE337126B (fr) 1971-07-26
FR2089383A5 (fr) 1972-01-07

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