US3747842A - Centrifuge rotor with sample holding means - Google Patents

Centrifuge rotor with sample holding means Download PDF

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Publication number
US3747842A
US3747842A US00172157A US3747842DA US3747842A US 3747842 A US3747842 A US 3747842A US 00172157 A US00172157 A US 00172157A US 3747842D A US3747842D A US 3747842DA US 3747842 A US3747842 A US 3747842A
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United States
Prior art keywords
openings
test tubes
flange
rotor
spaced
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Expired - Lifetime
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US00172157A
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English (en)
Inventor
J Grubb
K Tomei
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Hamilton Co Inc
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Hamilton Co Inc
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Publication date
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Publication of US3747842A publication Critical patent/US3747842A/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
    • B04B5/0421—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes pivotably mounted

Definitions

  • ABSTRACT A centrifuge rotor having a hollow concave bowl with a large axial column extending upwardly above a plane of the rim of the bowl and on which there is axially disposed a flange of smaller diameter than the diameter of the rim of the bowl and spaced upwardly of the plane of said rim, the flange having a plurality of annularlyarranged and spaced, radially-extending, elongated or oval-shaped openings.
  • a second and smaller flange Spaced above said flange is a second and smaller flange axially carried by said column and also having a plurality of radially-extending, annularly-arranged and spaced, elongated or oval openings aligned with respective oval openings of the larger lower flange.
  • the column is hollow and has a large recess in the lower portion with a reduced diameter recessed part extending upwardly from the upper end of said large recess.
  • a pair of walls extend laterally apart to provide space for reception of a cross pin of an upstanding centrifuge drive shaft, there being a bore extending upwardly from the upper end of the reduced diameter part of the recess and through an upstanding axial stem for stabilizing reception of a part of the drive shaft above the cross pin.
  • the oval openings in the flanges are adapted to operably receive test tubes therein and the webs between every other opening in the smaller, upper flange have a hook with a part embedded in the webs and an arm facing radially outwardly and spaced below the underside of the webs.
  • An elastic band of rubber, or other suitable resilient material, is carried by the hooks, the portions of the band between hooks extending across or crossing or spanning the openings intermediate the ends of said openings and closer to the inner ends of said openings than the outer ends.
  • the band portions are spaced from the inner ends of the openings a distance less than the diameter of test tubes operably disposed in said openings, so that said band parts or portions resiliently and yieldingly urge the test tubes inwardly against the walls defining the inner ends of said openings and hold same in Such position when the rotor is not operating, even when the rotor is turned upside down.
  • the band permits the test tubes to pivot on the walls defining the inner ends of said openings.
  • the lower part of said tubes are adapted to swing outwardly by centrifugal force when the rotor is rotated, the tubes extending through the openings in the lower flange which are longer than the openings in the upper flange to permit the lower part of such tubes to be thrown outwardly.
  • the lower flange has a depending flange which limits upward movement of the test tubes when the device is operably rotated and the bowl prevents the test tubes from being thrown out of the device.
  • the band portions or parts that cross or span the openings in the upper flange are spaced from the inner end walls of the openings a sufficient distance to permit the test tubes to be easily flipped into the inner portions of the openings of said upper flange but said test tubes are held within said openings, even when the device is inverted.
  • the invention relates generally to centrifuge rotors and refers more particularly to rotors for use in centrifuges used in making urinalyses.
  • the present invention comprises a centrifuge rotor having a hollow concave bowl with a large axial column extending upwardly above the plane of the rim of the bowl and on which there is axially disposed a flange of smaller diameter than the diameter of the rim of the bowl and spaced upwardly of the plane of said rim, the flange having a plurality of annularly-arranged and spaced, radially-extending, elongated or oval openings.
  • a second and smaller flange axially carried by said column also having a plurality of radially-extending, annularly-arranged and spaced, elongated or oval openings aligned with respective oval openings of the larger, lower flange.
  • the column is hollow and has a large recess in .the lower portion with a reduced diameter part extending upwardly in the upper end of said large recess, and at the upper end of the reduced diameter part of. the recess a pair of walls are spaced laterally apart to provide space for reception of a cross pin of an upstanding centrifuge drive shaft, there being a bore extending upwardly from the upper end of the reduced diameter part of the recess and through an upstanding axial stern for stabilizing reception of a part of the drive shaft above the cross pin.
  • the oval openings in the flanges are adapted to operably receive test tubes therein and the webs between every other opening in the smaller, upper flange have a hook with a part embedded in the webs and an arm facing radially outwardly and spaced below the underside of the webs.
  • An elastic band of rubber of other suitable material is carried by said hooks, the portions of the bands between the hooks extending across or crossing or spanning the bottoms of the openings intere mediate the ends of said openings and closer to the inner ends than the outer ends.
  • the band portions are spaced from the inner ends of the openings a distance less than the diameter of the test tubes 'operably disposed in said openings, so that said band parts or portions resiliently urge the test tubes inwardly against the walls defining the inner ends of said openings and hold same in such position when the rotor is not operating, even when the rotor is turned upside down.
  • the band permits the test tubes to pivot on the walls defining the inner ends of the openings of the upper flange with the lower parts of the tubes adapted to swing outwardly by centrifugal force when the rotor is rotated.
  • the tubes extend through the openings in the lower flange which are longer than the openings in the upper flange to permit the lower parts of such tubes to be thrown outwardly.
  • the lower flange has a depending flange which limits upward movement of the'test tubes when the device is operably rotated, and the bowl prevents the test tubes from being thrown out of the device.
  • the band portions extending across the openings in the upper flange are spaced sufficiently from the inner ends of the openings of said upper flange to permit easy insertion of said test tubes into the inner ends of the respective openings, but since the band parts are spaced from the inner ends of said openings a distance less than the diameter of test tubes used in the device, said test tubes will be held in position in the device when the rotor is not being rotated, even when the device is inverted, so that liquid in the testtubes may be simultaneously poured out of all of the tubes without the tubes being displaced.
  • test tubes may be easily and quickly inserted into openings provided therefor and retained in position.
  • Another object of the invention is to provide apparatus of this character wherein the sedimentin the urine in the test tubes is thrown to the. bottom under centrifugal force and the device then stopped and inverted to pour out the liquid in the test tubes so that the sediment may be removed from the test tubes and examined under a microscope.
  • Still another object of the invention is to provide apparatus of this character wherein the time for making protein tests and microscopic examinations is substantially reduced.
  • FIG. 1 is a side-elevational view of a centrifuge having a rotor embodying the present invention
  • FIG. 2 is a top plan view thereof;
  • FIG. 3 is a sectional view taken on line 3--3 of FIG.
  • FIG. 4 is an enlarged sectional view taken on line 4-4 of FIG. 1 showing test tubes therein as positioned when the rotor is not rotating;
  • FIG. 5 is a partial view similar to FIG. 4 but showing the test tubes positioned under the influence of centrifugal force.
  • FIG. 6 is an enlarged sectional view taken on line 6-6 of FIG. 2.
  • centrifuge apparatus including a base, indicated generally at 10, and a centrifuge rotor, indicated generally at 12.
  • the centrifuge rotor 12 may be of any suitable material such as, for example, a rigid plastic, of which there are various well known plastics on the market.
  • This rotor comprises a hollow concave bowl 14, from the bottom of which a large axial column or post 16 extends upwardly above the plane of the rim of the bowl. 0n the column 16 there is an integral flange 18 normal to the axis of the column 16, the flange 18 having a downwardly and outwardly-extending rim 20. Flange 18 with its rim 20 is spaced upwardly of the plane of the rim of the bowl 14 but is of smaller diameter than the diameter of the rim of the bowl, said rim being indicated at 16a.
  • flange 18 Spaced upwardly of the flange 18 is a smaller, upper flange 22, the upper flange 22 being normal to the axis of the column and parallel to the lower flange 18, the flange 22 also being integral with the column 16.
  • Lower flange 18 has a plurality of annularly-spaced, radially-arranged, elongated or oval openings 26 while the upper flange 22 has a similar number of elongated or oval openings 28 which are radially arranged and in alignment with the openings 26 of the lower flange 18.
  • These openings are adapted to operably receive test tubes 30 therein which are of well known character, being opened at the upper end and closed at the lower end by a rounded wall 32.
  • Within the base is means for rotating the rotor, said means being an electric motor, not shown, and current to the motor is controlled by a switch 34.
  • Extending axially upwardly from the base is a post 36, the upper end of which extends into a large, cylindrical, axially-arranged recess 38 in the lower portion of the column 16, said post 36 being operably received in the recess 38.
  • a reduceddiameter, axially-arranged recess 40 having a pair of oppositely-arranged walls 44 which are spaced apart and receive a cross pin 46 in a shaft 48 driven by the motor.
  • the walls 44 provide a driving connection between said shaft 48 and the rotor.
  • Shaft 48 extends upwardly of the cross pin 46 in a bore 50 which extends axially upwardly of the recess 40 and through a stem 52 which is integral with the rotor.
  • Post 36 is so received in the recess 38 to permit the rotor to freely rotate but stabilizes the rotor so that it will not wobble or vibrate when it is being rotated.
  • Means is provided for retaining the test tubes 30 in the rotor when the latter is inverted while permitting the test tubes to be pivoted adjacent their upper ends so that said test tubes will be swung to an inclined position with the closed'ends positioned outwardly under the influence of centrifugal force.
  • This means comprises a resilient, elastic band 56 which is shown as a rubber band.
  • Means for securing rubber band 56 in operable position comprises a plurality of hooks 58, there being a hook 58 for every other web 60 between the annularly-arranged and spaced openings 28 in the upper flange.
  • Each hook 58 comprises a vertical shank 62 that is embedded in a web 60 and an arm 64 that extends radially outwardly from the shank 62 and in downwardly spaced, parallel relationship to the underside of the smaller, upper flange 22.
  • the books 58 thus, open outwardly and the rubber band 56 is disposed on these hooks.
  • the parts of the rubber band between hooks extend across or span the openings 28 intermediate their ends, said band portions being normally spaced from the inner ends or inner end walls 66 of the openings 28 a distance less than the diameter of the test tubes 30. However, said band portions are spaced from said walls 66 a sufficient distance to pennit easy and quick insertion of the test tubes in the inner parts of said openings 28.
  • the stem 52 serves as a handle in raising or lowering the rotor and provides means to facilitate placing the rotor on the shaft 48 with the cross pin in the space between the walls 44 so that the rotor is rotated when the shaft 48 is rotated.
  • test tubes When it is desired to operate the apparatus test tubes are placed in the inner ends of the openings 28 so that the band portions between the hooks are stretched slightly and produce resilient pressure against the upper parts of the tubes to urge same against the inner walls 66 of said openings.
  • the band 56 is at the underside of the flange 22.
  • the test tubes also extend through the openings 26 of the flange 18 and the band 56 functions to urge said test tubes against the column 16 so that the tubes are in an upright or vertical position when the rotor is not being rotated.
  • the lower ends or end walls 32 of the test tubes rest on the inner surface 14a of the bowl 14.
  • each of the test tubes contains a sample or specimen to be tested or analyzed and, to pennit ready identification of the tubes, each opening 28 in the small upper flange is numbered, the numbers being on the beveled edge portion 22a of the upper flange 22, as best shown in FIG. 2.
  • test tubes When the rotor is rotated at a high speed to provide the centrifuge effect, the test tubes pivot on the upper edge 16a of the column 16, or pivot or rock on the inner end wall of the openings 28 of the upper flange 18, so that they take an outwardly and downwardly position, FIG. 5.
  • the outer end walls 26a of the slots 26 in the lower flange limit upward movement of the lower end of the test tubes, and the inner surface 14a of the bowl prevents the test tubes from being thrown outwardly under the influence of centrifugal force.
  • the rotor is turned upside down to dump the liquid in the test tubes.
  • the band 56 holds the test tubes in position in the rotor and prevents said tubes from falling out of the rotor when the liquid therein is poured or dumped out.
  • a centrifuge rotor for test tubes comprising:
  • said upper flange having a plurality of annularly arranged and spaced openings therein aligned with respective openings in the lower flange;
  • portions of the rubber band spanning the openings in the upper flange are spaced from the inner ends of said openings a distance which is substantially half the length thereof, said portions are spaced sufficiently from the inner ends of said openings to permittest tubes to be inserted in the inner end parts of said openings, said portions of the band adapted to resiliently urge test tubes inwardly.
  • openings in the lower flange are elongated, said openings being radially arranged and annularly spaced apart, the outer end walls of said openings being engageable by test tubes operably disposed in the openings in said upper and lower flanges when the rotor is operably rotated, said end walls limiting upward movement of said test tubes, the lower ends of said test tubes moving upwardly and outwardly on the inner surface of the bowl, said bowl limiting outward movement of said test tubes.

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US00172157A 1971-08-16 1971-08-16 Centrifuge rotor with sample holding means Expired - Lifetime US3747842A (en)

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US17215771A 1971-08-16 1971-08-16

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US (1) US3747842A (de)
JP (1) JPS4829061A (de)
DE (1) DE2205879A1 (de)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4301964A (en) * 1980-01-24 1981-11-24 Beckman Instruments, Inc. Swinging tube holder
US4817453A (en) * 1985-12-06 1989-04-04 E. I. Dupont De Nemours And Company Fiber reinforced centrifuge rotor
US5045047A (en) * 1989-07-17 1991-09-03 Zymark Corporation Automated centrifuge
WO2000021678A1 (fr) * 1998-10-14 2000-04-20 Fondation Jean Dausset-Ceph Dispositif de centrifugation pour automate de laboratoire
US20110160031A1 (en) * 2002-08-02 2011-06-30 Harvest Technologies Corporation Decanting centrifuge with vibration isolation
WO2013078560A1 (en) * 2011-11-29 2013-06-06 N-Werkz Inc. Planetary mill and method of milling
EP3888794A1 (de) * 2020-03-30 2021-10-06 Sysmex Corporation Zentrifuge und probenvorbereitungsvorrichtung

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6080734A (ja) * 1983-10-07 1985-05-08 Hitachi Ltd 液体試料自動分析装置
JP6588201B2 (ja) * 2014-12-01 2019-10-09 メディカテック株式会社 遠心機

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB505446A (en) * 1937-11-10 1939-05-10 Baird & Tatlock Ltd Improvements in and relating to centrifuges
US2739759A (en) * 1954-09-28 1956-03-27 Clay Adams Inc Centrifuge
US3133882A (en) * 1961-07-21 1964-05-19 Internat Equipment Company Centrifuges with retainers, retainers, and bottle stoppers for use therewith
US3142385A (en) * 1961-09-07 1964-07-28 James F Kahlenberg Culture tube holder
US3352427A (en) * 1965-08-12 1967-11-14 Wallace C Lawrence Volumetric flask rack
US3401876A (en) * 1966-07-25 1968-09-17 Dade Reagents Inc Mixing and decanting centrifuge
US3666171A (en) * 1969-10-20 1972-05-30 Sorvall Inc Ivan Swinging bucket centrifuge rotor

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB505446A (en) * 1937-11-10 1939-05-10 Baird & Tatlock Ltd Improvements in and relating to centrifuges
US2739759A (en) * 1954-09-28 1956-03-27 Clay Adams Inc Centrifuge
US3133882A (en) * 1961-07-21 1964-05-19 Internat Equipment Company Centrifuges with retainers, retainers, and bottle stoppers for use therewith
US3142385A (en) * 1961-09-07 1964-07-28 James F Kahlenberg Culture tube holder
US3352427A (en) * 1965-08-12 1967-11-14 Wallace C Lawrence Volumetric flask rack
US3401876A (en) * 1966-07-25 1968-09-17 Dade Reagents Inc Mixing and decanting centrifuge
US3666171A (en) * 1969-10-20 1972-05-30 Sorvall Inc Ivan Swinging bucket centrifuge rotor

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4301964A (en) * 1980-01-24 1981-11-24 Beckman Instruments, Inc. Swinging tube holder
US4817453A (en) * 1985-12-06 1989-04-04 E. I. Dupont De Nemours And Company Fiber reinforced centrifuge rotor
US5045047A (en) * 1989-07-17 1991-09-03 Zymark Corporation Automated centrifuge
WO2000021678A1 (fr) * 1998-10-14 2000-04-20 Fondation Jean Dausset-Ceph Dispositif de centrifugation pour automate de laboratoire
FR2785206A1 (fr) * 1998-10-14 2000-05-05 Fondation Jean Dausset Ceph Dispositif de centrifugation pour automate de laboratoire
US6872360B1 (en) 1998-10-14 2005-03-29 Fondation Jean Dausset-Ceph Centrifuging device for laboratory analyzer
US20110160031A1 (en) * 2002-08-02 2011-06-30 Harvest Technologies Corporation Decanting centrifuge with vibration isolation
US8152708B2 (en) * 2002-08-02 2012-04-10 Harvest Technologies Corporation Decanting centrifuge with sliding engagement between decant ring and processing unit
WO2013078560A1 (en) * 2011-11-29 2013-06-06 N-Werkz Inc. Planetary mill and method of milling
CN103974775A (zh) * 2011-11-29 2014-08-06 N-威尔克兹公司 行星式研磨机以及研磨方法
US9221057B2 (en) 2011-11-29 2015-12-29 N-Werkz Inc. Planetary mill and method of milling
EP2785463A4 (de) * 2011-11-29 2016-03-02 Werkz Inc N Planetenwalzwerk und walzverfahren
US9446413B2 (en) 2011-11-29 2016-09-20 N-Werkz Inc. Planetary mill and method of milling
CN103974775B (zh) * 2011-11-29 2017-03-01 N-威尔克兹公司 行星式研磨机以及研磨方法
EP3888794A1 (de) * 2020-03-30 2021-10-06 Sysmex Corporation Zentrifuge und probenvorbereitungsvorrichtung

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Publication number Publication date
DE2205879A1 (de) 1973-08-02
JPS4829061A (de) 1973-04-17

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