US3686073A - Fermentor - Google Patents

Fermentor Download PDF

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Publication number
US3686073A
US3686073A US858590A US3686073DA US3686073A US 3686073 A US3686073 A US 3686073A US 858590 A US858590 A US 858590A US 3686073D A US3686073D A US 3686073DA US 3686073 A US3686073 A US 3686073A
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United States
Prior art keywords
lid
fermentor
vessel
glass vessel
hollow cylindrical
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Expired - Lifetime
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US858590A
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Achilles Baerfuss
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AG fur BIOLOGISCHE VERFAHRENSTECHNIK
fur BIOLOG VERFAHRENSTECHNIK A
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fur BIOLOG VERFAHRENSTECHNIK A
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D51/00—Closures not otherwise provided for
    • B65D51/14—Rigid discs or spherical members adapted to be held in sealing engagement with mouth of container, e.g. closure plates for preserving jars
    • B65D51/145—Rigid discs or spherical members adapted to be held in sealing engagement with mouth of container, e.g. closure plates for preserving jars by means of an additional element connected directly to the container
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings

Definitions

  • Sealing rings one between the sleeve and the neck, and one between the sleeve and lid, ensure hermetic sealing of the vessel.
  • the lid is retained by a securing ring engaging the sleeve for quick assembly of the fermentor and for withstanding high pressure in the vessel.
  • This invention relates to fermentors.
  • fermentors In a fermentor it is required that the glass vessel and the lid should be connected together so tightly that equalisation of pressure with the outside is not possible either when a super-atmospheric pressure or a subatmospheric pressure exists inside the glass vessel.
  • fermentors have been provided comprising a glass vessel having a ange provided with a ground surface on which the lid rests secured by means of a screw closure device, and a sealing element is disposed between the lid and the ground surface.
  • a disadvantage of these fermentors is that for Sterilisation they must be introduced into an autoclave. This is because small volume fermentors are not usually provided with a closure dev-ice capable of withstanding the pressure occurring in the interior during Sterilisation.
  • screw closure devices so dimensioned to withstand said pressure, the dimensions of the fermentor would exceed the dimensions of apparatus usually employed in laboratories, and additional tools and suitable servicing personnel would be necessary.
  • a fermentor comprising a glass vessel, a cylindrical opening to the glass vessel, edge to the cylindrical opening being thickened radially outwards, a lid, a retaining device for securing the lid on the vessel, a sealing ring disposed between the thickened edge and the retaining device, and a sealing ring disposed between an edge of the lid and the retaining device.
  • the fermentor further comprises a hollow cylindrical body providing the retaining device, said hollow cylindrical body surrounding the lid and a part of the glass vessel, a supporting ring disposed between said body and the glass wall, a carrier element of thermally insulating material supported on said supporting ring, a groove provided in a rim portion of the hollow cylindrical body, a securing fastening ring extending into said groove thereby pre venting the lid being removed from the vessel.
  • the novel fermentor makes it possible to dispense with the use of screws and the tools associated therewith. Additionally this construction provides a fermentor having a high speed closure, that is to say a closure device which can be opened and closed rapidly and without the aid of tools.
  • glass vessels of this type can be delivered from stock by specialist dealers, so that it is not necessary yfor the user to maintain his own stock.
  • the fermentor illustrated in FIG. 1 comprises a glass vessel 1, a metal lid 2, and a retaining device 3 for securing the lid 2 on the glass vessel 1.
  • the glass vessel 1, which has a cylindrical opening, is provided with a thickened edge 11 the thickened portion of which extends radially outwards.
  • the thickened edge 11 has a surface 11a which is divergent in the direction of the lid 2, while a surface 11b forms with said surface 11a an external angle of more than
  • the lid 2 is provided with thermaly insulating spacers 7, which 0n the one hand are inserted into bores 2b in said lid 2 and on the other and rest on the edge 11e ⁇ of the glass vessel 1, so that between the inner surface of the metal lid 2 and glass edge llc an insulatnig air layer is formed.
  • the lid 2 is supported on a shoulder 3b on the hollow cylindrical body 3. Between the edge of the lid 2 and the hollow cylindrical 'body 3 of the retaining device there is disposed a sealing ring 9, which is mounted in a groove 2a in the lid 2.
  • a sealing ring 6 is likewise disposed between the surface 11a of the thickened portion 11 of the glass and the hollow cylindrical body 3 of the retaining device, the ring being mounted in a groove 3c.
  • the hollow cylindrical body 3 surrounds the lid 2 and a part of the glass vessel 1, while between said body and the glass wall there is dipsosed a multipart supporting ring 5, which carries a carrier element 4 of thermal insulating material and is mounted in a groove 3d.
  • a fastening ring S which partly extends into a groove 3a provided in the hollow cylindrical body 3 in order to prevent the movement of the lid perpendicularly to its surface away from the vessel 1.
  • the vessel 1 closed by the lid 2 can be opened easily and rapidly.
  • the fastening ring 8 is loosened and the lid 2 together with the sealing ring 9 and the spacers 7 are pulled olf the vessel.
  • the glass vessel can easily be extracted from the hollow cylindrical body 3.
  • the surface 11a of the thickened portion 11 of the glass must extend in such a manner that the sealing ring 6 is pressed into the groove 3c when the glass vessel 1 is closed.
  • the advantage of a form of sealing of this type consists firstly in that the glass vessel can be sealed without the use of screws, since this construction forms a rapid closure device for the lid 2, which can be closed without the application of any particular force in such a manner that it will also withstand the pressures which occur during Sterilisation of its interior; secondly, this type of sealing permits simple replacement of the vessels, since the vessels can immediately be lifted out of the whole cylindrical body. It is thus possible, for example, to prepare a nutrient medium in a vessel anywhere in the establishment and then to insert the vessel prepared in this manner and use it as a component of the fermentor and subsequently to forward it elsewhere with its contents complete without it being necessary for the contents to be transferred to another vessel.
  • a fermentor comprising, in combination, a glass vessel provided with a substantially cylindrical opening, said glass vessel incorporating a radially outwardly thickened edge disposed externally of said cylindrical opening of said glass vessel, a lid for closing said glass vessel, a retaining device for securing said lid on said glass vessel, a first sealing ring disposed between said thickened edge of said glass vessel and said retaining device, said lid including an edge, and a second sealing ring disposed between said edge of said lid and said retaining device.
  • said retaining device comprising a substantially hollow cylindrical body surrounding said lid and part of said glass vessel, saidl glass vessel including glass wall means, a supporting ring disposed between said hollow cylindrical body and said glass wall means of said glass vessel, a carrier element of thermally insulating material supported on said supporting ring, said hollow cylindrical body including a rim portion provided with a groove, a securing fastening ring extending into said groove at said rim portion of said hollow cylindrical body for preventing said lid from being unintentionally removed from said glass vessel.

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Clinical Laboratory Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Genetics & Genomics (AREA)
  • Mechanical Engineering (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Packages (AREA)
  • Closures For Containers (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A FERMENTOR COMPRISING A GLASS VESSEL WITH AN OPENING HAVING A RADIALLY EXTENDING THICKENED NECK PORTION. A HOLLOW CYLINDRICAL SLEEVE SURROUNDS THE NECK AND A LID IS SEATED ON THE NECK. SEALING RINGS, ONE BETWEEN THE SLEEVE AND THE NECK, AND ONE BETWEEN THE SLEEVE AND LID, ENSURE HERMETIC SEALING OF THE VESSEL. THE LID IS RETAINED BY A SECURING RING ENGAGING THE SLEEVE FOR QUICK ASSEMBLY OF THE FERMENTOR AND FOR WITHSTANDING HIGH PRESSURE IN THE VESSEL.

Description

ug- 22 1972 A. BAERr-'uss 3,686,073
FERMENTOR Filed Sept. 17. 1969 L; A l
United States Patent O 3,686,073 FERMENTOR Achilles Baerfuss, Oberwil, Switzerland, assignor to Aktiengesellschaft fr Biologische Verfahrenstechnik, Basel, Switzerland Filed Sept. 17, 1969, Ser. No. 858,590 -Claims priority, application Switzerland, May 7, 1969, 7,031/69 Int. Cl. C12 b 1 00 U.S. Cl. 195-139 8 Claims ABSTRACT F THE DISCLOSURE A fermentor comprising a glass vessel with an opening having a radially extending thickened neck portion. A hollow cylindrical sleeve surrounds the neck and a lid is seated on the neck. Sealing rings, one between the sleeve and the neck, and one between the sleeve and lid, ensure hermetic sealing of the vessel. The lid is retained by a securing ring engaging the sleeve for quick assembly of the fermentor and for withstanding high pressure in the vessel.
BACKGROUND TO THE INVENTION This invention relates to fermentors.
In a fermentor it is required that the glass vessel and the lid should be connected together so tightly that equalisation of pressure with the outside is not possible either when a super-atmospheric pressure or a subatmospheric pressure exists inside the glass vessel. Hitherto, fermentors have been provided comprising a glass vessel having a ange provided with a ground surface on which the lid rests secured by means of a screw closure device, and a sealing element is disposed between the lid and the ground surface. A disadvantage of these fermentors is that for Sterilisation they must be introduced into an autoclave. This is because small volume fermentors are not usually provided with a closure dev-ice capable of withstanding the pressure occurring in the interior during Sterilisation. Although it is possible to design screw closure devices so dimensioned to withstand said pressure, the dimensions of the fermentor would exceed the dimensions of apparatus usually employed in laboratories, and additional tools and suitable servicing personnel would be necessary.
SUMMARY OF THE INVENTION According to the present invention there is provided a fermentor comprising a glass vessel, a cylindrical opening to the glass vessel, edge to the cylindrical opening being thickened radially outwards, a lid, a retaining device for securing the lid on the vessel, a sealing ring disposed between the thickened edge and the retaining device, and a sealing ring disposed between an edge of the lid and the retaining device. Preferably, the fermentor further comprises a hollow cylindrical body providing the retaining device, said hollow cylindrical body surrounding the lid and a part of the glass vessel, a supporting ring disposed between said body and the glass wall, a carrier element of thermally insulating material supported on said supporting ring, a groove provided in a rim portion of the hollow cylindrical body, a securing fastening ring extending into said groove thereby pre venting the lid being removed from the vessel. The novel fermentor makes it possible to dispense with the use of screws and the tools associated therewith. Additionally this construction provides a fermentor having a high speed closure, that is to say a closure device which can be opened and closed rapidly and without the aid of tools. In addition, glass vessels of this type can be delivered from stock by specialist dealers, so that it is not necessary yfor the user to maintain his own stock.
3,686,073 Patented Aug. 22, 1972 ice BRIEF DESCRIPTION OF THE DRAWING DESCRIPTION `OF THE INVENTION The fermentor illustrated in FIG. 1 comprises a glass vessel 1, a metal lid 2, and a retaining device 3 for securing the lid 2 on the glass vessel 1. The glass vessel 1, which has a cylindrical opening, is provided with a thickened edge 11 the thickened portion of which extends radially outwards. The thickened edge 11 has a surface 11a which is divergent in the direction of the lid 2, while a surface 11b forms with said surface 11a an external angle of more than The lid 2 is provided with thermaly insulating spacers 7, which 0n the one hand are inserted into bores 2b in said lid 2 and on the other and rest on the edge 11e` of the glass vessel 1, so that between the inner surface of the metal lid 2 and glass edge llc an insulatnig air layer is formed. In addition, the lid 2 is supported on a shoulder 3b on the hollow cylindrical body 3. Between the edge of the lid 2 and the hollow cylindrical 'body 3 of the retaining device there is disposed a sealing ring 9, which is mounted in a groove 2a in the lid 2. A sealing ring 6 is likewise disposed between the surface 11a of the thickened portion 11 of the glass and the hollow cylindrical body 3 of the retaining device, the ring being mounted in a groove 3c. The hollow cylindrical body 3 surrounds the lid 2 and a part of the glass vessel 1, while between said body and the glass wall there is dipsosed a multipart suporting ring 5, which carries a carrier element 4 of thermal insulating material and is mounted in a groove 3d. Along the edge of the lid 2 there is disposed a fastening ring S which partly extends into a groove 3a provided in the hollow cylindrical body 3 in order to prevent the movement of the lid perpendicularly to its surface away from the vessel 1.
The vessel 1 closed by the lid 2 can be opened easily and rapidly. For this purpose the fastening ring 8 is loosened and the lid 2 together with the sealing ring 9 and the spacers 7 are pulled olf the vessel. The glass vessel can easily be extracted from the hollow cylindrical body 3. The surface 11a of the thickened portion 11 of the glass must extend in such a manner that the sealing ring 6 is pressed into the groove 3c when the glass vessel 1 is closed.
The advantage of a form of sealing of this type consists firstly in that the glass vessel can be sealed without the use of screws, since this construction forms a rapid closure device for the lid 2, which can be closed without the application of any particular force in such a manner that it will also withstand the pressures which occur during Sterilisation of its interior; secondly, this type of sealing permits simple replacement of the vessels, since the vessels can immediately be lifted out of the whole cylindrical body. It is thus possible, for example, to prepare a nutrient medium in a vessel anywhere in the establishment and then to insert the vessel prepared in this manner and use it as a component of the fermentor and subsequently to forward it elsewhere with its contents complete without it being necessary for the contents to be transferred to another vessel.
il claim:
1. A fermentor comprising, in combination, a glass vessel provided with a substantially cylindrical opening, said glass vessel incorporating a radially outwardly thickened edge disposed externally of said cylindrical opening of said glass vessel, a lid for closing said glass vessel, a retaining device for securing said lid on said glass vessel, a first sealing ring disposed between said thickened edge of said glass vessel and said retaining device, said lid including an edge, and a second sealing ring disposed between said edge of said lid and said retaining device.
2. A fermentor as defined in claim 1, said retaining device comprising a substantially hollow cylindrical body surrounding said lid and part of said glass vessel, saidl glass vessel including glass wall means, a supporting ring disposed between said hollow cylindrical body and said glass wall means of said glass vessel, a carrier element of thermally insulating material supported on said supporting ring, said hollow cylindrical body including a rim portion provided with a groove, a securing fastening ring extending into said groove at said rim portion of said hollow cylindrical body for preventing said lid from being unintentionally removed from said glass vessel.
3. A fermentor as defined in claim 2, wherein said hollow cylindrical body comprises a groove for accommodating said supporting ring, and a groove retaining said first sealing ring disposed between said hollow cylindrical body and said thickened edge of said glass vessel.
4. A fermentor as defined in claim 3, wherein said groove retaining said first sealing ring is provided at said hollow cylindrical body, and said first sealing ring when positioned in said groove provided therefor being disposed immediately between and in direct contact with said hollow cylindrical body and said thickened portion of said glass vessel respectively.
5. A fermentor as defined in claim 1, wherein said edge of said lid is provided with a groove receiving said second sealing ring.
6. A fermentor as defined in claim 5, wherein said edge of said lid provided with said groove for said second sealing ring defines a peripheral edge, and said second sealing ring when disposed in said last-mentioned groove of said lid being located immediately between and in direct contact with said retaining device and said lid.
7. A fermentor as defined in claim 1, wherein said thickened portion of said glass vessel possesses a surface which is in contact with said first sealing ring, said thickened portion surface being outwardly divergent in the direction of said lid.
8. A fermentor as defined in claim 1, wherein said lid is formed of metal, said retaining device being provided with shoulder means for supporting said metallic lid, said glass vessel having an upper edge, and thermally insulating spacers resting upon said upper edge of said glass vessel.
References Cited UNITED STATES PATENTS 1,963,675 6/1934 Plainevaux et al. 220-55 AN 3,063,594 11/1962 Gerard et al. 220-55 AN 3,483,089 12/ 1969 Brewer 195-127 ALVIN E. TANENHOLTZ, Primary Examiner U.S. C1. X.R. 220--46
US858590A 1969-05-07 1969-09-17 Fermentor Expired - Lifetime US3686073A (en)

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CH703169A CH508046A (en) 1969-05-07 1969-05-07 Fermenter

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CH (1) CH508046A (en)
DE (2) DE2022575C3 (en)
FR (1) FR2047492A5 (en)
GB (1) GB1258864A (en)
SE (1) SE358410B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4595038A (en) * 1982-02-08 1986-06-17 Bolenz & Schafer Maschinenfabrik Zweigniederlassung der Rexnard GmbH Pressure accumulator and vessel thereof
US20060065676A1 (en) * 2004-09-23 2006-03-30 Quantum Fuel Systems Technologies Worldwide, Inc. Tank seal

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4595038A (en) * 1982-02-08 1986-06-17 Bolenz & Schafer Maschinenfabrik Zweigniederlassung der Rexnard GmbH Pressure accumulator and vessel thereof
US20060065676A1 (en) * 2004-09-23 2006-03-30 Quantum Fuel Systems Technologies Worldwide, Inc. Tank seal
WO2006034439A2 (en) * 2004-09-23 2006-03-30 Quantum Fuel Systems Technologies Worldwide, Inc. Tank seal
WO2006034439A3 (en) * 2004-09-23 2010-01-28 Quantum Fuel Systems Technologies Worldwide, Inc. Tank seal
CN101432602B (en) * 2004-09-23 2010-11-10 昆腾燃料系统技术公司 Tank seal
US7837054B2 (en) * 2004-09-23 2010-11-23 Quantum Fuel Systems Technologies Worldwide, Inc. Tank seal

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Publication number Publication date
DE2022575C3 (en) 1973-09-27
SE358410B (en) 1973-07-30
DE2022575A1 (en) 1970-11-12
DE2022575B2 (en) 1973-02-15
FR2047492A5 (en) 1971-03-12
GB1258864A (en) 1971-12-30
CH508046A (en) 1971-05-31
DE7017348U (en) 1970-08-27

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