US3649218A - Method for preparing metered liquid sample dilutions - Google Patents

Method for preparing metered liquid sample dilutions Download PDF

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Publication number
US3649218A
US3649218A US822488A US3649218DA US3649218A US 3649218 A US3649218 A US 3649218A US 822488 A US822488 A US 822488A US 3649218D A US3649218D A US 3649218DA US 3649218 A US3649218 A US 3649218A
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US
United States
Prior art keywords
diluent
metered
volume
conduit
concentrate
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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US822488A
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English (en)
Inventor
Jacques A Pontigny
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Coulter Electronics Inc
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Coulter Electronics Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/0318—Processes
    • Y10T137/0391—Affecting flow by the addition of material or energy

Definitions

  • This invention is directed to method and apparatus for preparing precise, high ratio dilutions of fluids, and is especially well adapted for biological processing of micro quantities of a concentrate or sample in the absence of intersample contamination and intermediate system rinsings.
  • Diluting operations are common to laboratories, scientific, industrial and medical. When dilutions are prepared manually, their precision depends on the skill of the operator in charge of this tedious and long work. Consequently, the use of automatic diluting apparatus has been contemplated and employed for sometime. Those known up to the present time employ complicated and costly mechanisms, such as suction or pressure pumps, a complicated valve system and other delicate means. These apparatus are therefore fragile and poorly adapted to mass diluting operations, as for example, in the case of hematology in which the counting of white corpuscles requires a first dilution followed by a second dilution for counting the red corpuscles with respect to a very large number of specimens.
  • prior diluting apparatus and methods have the drawback of drawing inthe liquid to be diluted and discharging the diluted mixture through the same passage.
  • the invention remedies the prior art drawbacks and its object is to provide an automatic, simple, reliable and precise diluting method and apparatus which is easily and rapidly employed and avoids the aforementioned pollution.
  • the diluting apparatus provides a first tubular element having an inlet for the diluent and an outlet for the diluted liquid, a pumping device located at said inlet for circulating a metered volume of diluent in the first element, a second tubular element for the sample to be diluted including a capillary zone of given capacity, means for filling the zone with sample, conduits interconnecting the first and second tubular elements at the ends of the zone, so that the zone forms a fluid bypass and means for preventing the diluent from entering the zone when the sample is introduced therein.
  • FIGURE is a diagrammatic side elevation view, partly in section, of one embodiment of a diluting apparatus according to the invention.
  • a first tubular element 1, through which the diluent and dilution flows, and a second tubular element 2 through which flows the sample or concentrate to be diluted, for example blood, comprise the two major flow portions of the apparatus. Both of these elements can be of a rigid material such as glass.
  • a metering pump comprising a cylinder 4, a piston 5 retained in its lower position by a return spring 6, a cam 7, and a motor 8, for driving the cam to urge the piston 5 toward the top of the cylinder.
  • This pump is coupled to a source of diluent (not shown) by way of a conduit 9 having a vertical section in which a ball valve 10 is placed.
  • a vertical branch 11 Intermediate the ends of the tubular element 1 is a vertical branch 11, at the ends of which are two capillary conduits 12 and 13. Between the two conduits 12 and 13, the branch 11 has a region of reduced diameter or a constriction 14. A ball valve 15 is disposed in the branch 11 between the two conduits12 and 13.
  • Two other ball valves 16 and 17 are respectively located in the element 1 upstream and downstream of the branch 11.
  • the element 1 terminates in an outlet 18 which can empty into a dilution receiving vessel 19.
  • the tubular element 2 has a capillary zone 20, of given capacity, the ends of which are connected to the two conduits 12 and 13.
  • the element 2 is fed with sample, such as blood, from a source 21 located at one of its ends 22.
  • An aspirator or equivalent device 23 is provided at the other end 24.
  • the second tubular element 2 also includes shutofi' valves 25 near its ends.
  • the apparatus operates in the following manner.
  • tubular element 1 and the conduits 12, 13 are filled with diluent and the tubular element 2 with concentrate sample.
  • the valves 25 are closed and the piston 5 is in its lower position.
  • the cam 7 urges the piston 5 to its upper position and this delivers a given amount of diluent to the element 1.
  • This amount of diluent displaces an equal amount of diluent already contained in the element 1 and the latter amount flows partly through the branch 11 and, owing to the constriction 14, partly through the bypass consisting of the conduit 13, the zone 20 and the conduit 12.
  • the zone 20 is capillary, the small amount of sample it initially contained is completely urged along as soon as the diluent starts to arrive in this zone 20.
  • the mixture thus formed of a given amount of diluent and the amount of sample contained in the zone 20 drops into the vessel 19.
  • the dilution is thus effected.
  • the capillary zone 20 could have a 40 microliter volume, and the cylinder 4 would then have a 20.04 milliliter volume; such that each precise dilution would have the volume of 20.04 milliliters, of which 40 microliters is the blood sample.
  • volume of the cylinder 4 can, be adjustable, depending upon the lowest position of the piston 5.
  • volume of the capillary zone 20 can be varied by making the zone an interchangeable element; whereby, substitution of different zone elements of different volume can be accomplished.
  • a method for sequentially preparing precise uncontaminated liquid dilutions from liquid sample concentrate, each sequence comprising the steps of:

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
US822488A 1968-05-08 1969-05-07 Method for preparing metered liquid sample dilutions Expired - Lifetime US3649218A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR150945 1968-05-08

Publications (1)

Publication Number Publication Date
US3649218A true US3649218A (en) 1972-03-14

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US822488A Expired - Lifetime US3649218A (en) 1968-05-08 1969-05-07 Method for preparing metered liquid sample dilutions

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US (1) US3649218A (US06521211-20030218-C00004.png)
DE (1) DE1923153C3 (US06521211-20030218-C00004.png)
FR (1) FR1585020A (US06521211-20030218-C00004.png)
GB (1) GB1263776A (US06521211-20030218-C00004.png)
NL (1) NL6906968A (US06521211-20030218-C00004.png)
SE (1) SE365047B (US06521211-20030218-C00004.png)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3831618A (en) * 1972-12-22 1974-08-27 Abbott Lab Apparatus for the precision metering of fluids
US3865549A (en) * 1971-10-07 1975-02-11 Wellcome Found Auto-analytical apparatus and analytical methods
US4059408A (en) * 1976-03-12 1977-11-22 Commissariat A L'energie Atomique Automatic liquid-liquid extraction device
US4528158A (en) * 1982-06-14 1985-07-09 Baird Corporation Automatic sampling system
US4610170A (en) * 1983-11-30 1986-09-09 Labsystems Oy Method for the dilution of liquid samples
EP0263021A2 (en) * 1986-09-30 1988-04-06 National Aeronautics And Space Administration Infusion extractor
EP0311588A2 (en) * 1987-10-09 1989-04-12 Medonic Ingemar Berntsson Ab Method and apparatus for volumetric determination, dilution and transfer of liquid specimens
US6284548B1 (en) * 1998-02-06 2001-09-04 Boule Medical Ab Blood testing method and apparatus
US20040208794A1 (en) * 2002-08-13 2004-10-21 Karg Jeffrey A. Microfluidic mixing and dispensing
US20050181519A1 (en) * 2004-02-17 2005-08-18 Karg Jeffrey A. Metering doses of sample liquids
CN104897820A (zh) * 2015-05-22 2015-09-09 中国科学院寒区旱区环境与工程研究所 一种在线样品稀释中和试验装置

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS55140156A (en) * 1979-04-19 1980-11-01 Olympus Optical Co Ltd Distribution method

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR807852A (fr) * 1935-10-05 1937-01-23 Minimax Ag Dispositif pour introduire des produits tels que des extraits ou similaires, etc., dans des tuyauteries sous pression, en particulier pour l'obtention de mousses extinctrices d'incendies
US2332030A (en) * 1941-03-25 1943-10-19 Esther C Toronto Medicine mixing and dispensing device
US2607718A (en) * 1946-06-17 1952-08-19 Petrolite Corp Process and apparatus for control of reagents
US2633472A (en) * 1947-10-06 1953-03-31 Petrolite Corp Reagent control method and apparatus
US2984250A (en) * 1957-09-16 1961-05-16 Eugene L Foster Water treating system
US3052525A (en) * 1957-12-27 1962-09-04 Vogelmann Ernst Apparatus for introducing controlled quantities of liquids and solutes into a fluid medium
US3085717A (en) * 1961-04-24 1963-04-16 Vyzk Ustav Energeticky Automatic pipette
US3152117A (en) * 1957-11-27 1964-10-06 Stamicarbon Process for feeding a liquid into a turbulent second liquid
US3166096A (en) * 1961-10-03 1965-01-19 Lang Helmut Dispenser for liquid additives to fluid streams
US3199956A (en) * 1962-10-18 1965-08-10 Technicon Instr Cocurrent packed helical coil extractor
US3334018A (en) * 1962-09-05 1967-08-01 Technicon Corp Means for analyzing a continuous stream of unique sanguineous samples
US3367849A (en) * 1964-03-20 1968-02-06 Atomic Energy Commission Usa Amperometric determination of glucose
US3486859A (en) * 1966-10-11 1969-12-30 Greiner Electronic Ag Blood analyzing method and apparatus

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR807852A (fr) * 1935-10-05 1937-01-23 Minimax Ag Dispositif pour introduire des produits tels que des extraits ou similaires, etc., dans des tuyauteries sous pression, en particulier pour l'obtention de mousses extinctrices d'incendies
US2332030A (en) * 1941-03-25 1943-10-19 Esther C Toronto Medicine mixing and dispensing device
US2607718A (en) * 1946-06-17 1952-08-19 Petrolite Corp Process and apparatus for control of reagents
US2633472A (en) * 1947-10-06 1953-03-31 Petrolite Corp Reagent control method and apparatus
US2984250A (en) * 1957-09-16 1961-05-16 Eugene L Foster Water treating system
US3152117A (en) * 1957-11-27 1964-10-06 Stamicarbon Process for feeding a liquid into a turbulent second liquid
US3052525A (en) * 1957-12-27 1962-09-04 Vogelmann Ernst Apparatus for introducing controlled quantities of liquids and solutes into a fluid medium
US3085717A (en) * 1961-04-24 1963-04-16 Vyzk Ustav Energeticky Automatic pipette
US3166096A (en) * 1961-10-03 1965-01-19 Lang Helmut Dispenser for liquid additives to fluid streams
US3334018A (en) * 1962-09-05 1967-08-01 Technicon Corp Means for analyzing a continuous stream of unique sanguineous samples
US3199956A (en) * 1962-10-18 1965-08-10 Technicon Instr Cocurrent packed helical coil extractor
US3367849A (en) * 1964-03-20 1968-02-06 Atomic Energy Commission Usa Amperometric determination of glucose
US3486859A (en) * 1966-10-11 1969-12-30 Greiner Electronic Ag Blood analyzing method and apparatus

Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3865549A (en) * 1971-10-07 1975-02-11 Wellcome Found Auto-analytical apparatus and analytical methods
US3831618A (en) * 1972-12-22 1974-08-27 Abbott Lab Apparatus for the precision metering of fluids
US4059408A (en) * 1976-03-12 1977-11-22 Commissariat A L'energie Atomique Automatic liquid-liquid extraction device
US4528158A (en) * 1982-06-14 1985-07-09 Baird Corporation Automatic sampling system
US4610170A (en) * 1983-11-30 1986-09-09 Labsystems Oy Method for the dilution of liquid samples
EP0263021A2 (en) * 1986-09-30 1988-04-06 National Aeronautics And Space Administration Infusion extractor
EP0263021A3 (en) * 1986-09-30 1989-11-15 National Aeronautics And Space Administration Infusion extractor
EP0311588A2 (en) * 1987-10-09 1989-04-12 Medonic Ingemar Berntsson Ab Method and apparatus for volumetric determination, dilution and transfer of liquid specimens
EP0311588A3 (en) * 1987-10-14 1990-09-05 Medonic Ingemar Berntsson Ab Method and apparatus for volumetric determination, dilution and transfer of liquid specimens
US6284548B1 (en) * 1998-02-06 2001-09-04 Boule Medical Ab Blood testing method and apparatus
US20040208794A1 (en) * 2002-08-13 2004-10-21 Karg Jeffrey A. Microfluidic mixing and dispensing
US7459128B2 (en) 2002-08-13 2008-12-02 Molecular Bioproducts, Inc. Microfluidic mixing and dispensing
US20050181519A1 (en) * 2004-02-17 2005-08-18 Karg Jeffrey A. Metering doses of sample liquids
US7592185B2 (en) 2004-02-17 2009-09-22 Molecular Bioproducts, Inc. Metering doses of sample liquids
US20100008827A1 (en) * 2004-02-17 2010-01-14 Molecular BioProducts, Inc. a Delaware corporation Metering doses of sample liquids
US20110027906A1 (en) * 2004-02-17 2011-02-03 MOLECULAR BIOPRODUCTS, INC., a California corporation Metering doses of sample liquids
US8043865B2 (en) 2004-02-17 2011-10-25 Molecular Bioproducts, Inc. Metering doses of sample liquids
US8080218B2 (en) 2004-02-17 2011-12-20 Molecular Bio-Products, Inc. Metering doses of sample liquids
CN104897820A (zh) * 2015-05-22 2015-09-09 中国科学院寒区旱区环境与工程研究所 一种在线样品稀释中和试验装置

Also Published As

Publication number Publication date
DE1923153B2 (de) 1973-07-19
DE1923153C3 (de) 1974-02-21
SE365047B (US06521211-20030218-C00004.png) 1974-03-11
GB1263776A (en) 1972-02-16
DE1923153A1 (de) 1969-11-20
NL6906968A (US06521211-20030218-C00004.png) 1969-11-11
FR1585020A (US06521211-20030218-C00004.png) 1970-01-09

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