Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
  • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
  • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
  • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
  • G01N33/521—Single-layer analytical elements
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10S435/00—Chemistry: molecular biology and microbiology
  • Y10S435/805—Test papers
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
  • Y10T436/00—Chemistry: analytical and immunological testing
  • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
  • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
  • Y10T436/143333—Saccharide [e.g., DNA, etc.]
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
  • Y10T436/00—Chemistry: analytical and immunological testing
  • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
  • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
  • Y10T436/143333—Saccharide [e.g., DNA, etc.]
  • Y10T436/144444—Glucose

Definitions

• This invention relates to a diagnostic agent for the detection of biochemical and chemical components in whole blood and more particularly relates to a test strip for use in the detection of chemical and biochemical components in whole blood, a process for the preparation of such test strips and a method for using the same.
• a further object of the invention is to provide a new and improved test paper strip for use in detecting chemical and biochemical components of whole blood characterized by the extreme ease with which they are produced and used.
• agents which can be used for the hydrophobing of the absorbent carriers are natural and synthetic fats, oils and waxes, as well as, and preferably, paraflins, silicones and mixtures thereof.
• agents which can be used for the hydrophobing of the absorbent carriers are natural and synthetic fats, oils and waxes, as well as, and preferably, paraflins, silicones and mixtures thereof.
• test paper strips can be hydrophobed following impregnation with the indicator solutions.
• dependable and satisfactory test paper strips are also obtained when the indicator dyestuff, together with the hydrophobing agent, is employed as impregnant being applied onto test paper strips which have already been impregnated with the other reagents.
• the hydrophobed diagnostic agents there can be used not only filter paper, but also other absorbent carriers, such as asbestos paper and Wood rodlets.
• other absorbent carriers such as asbestos paper and Wood rodlets.
• the pores of the wood can sometimes be disturbing since red blood corpuscles collect therein and can only be wiped off or rinsed out with difficulty.
• the diagnostic agents according to the present invention can be utilized for all of the clinical tests which are of importance in the analysis of blood, especially for the clinical screening tests, as for example, the determination of glucose, galactose, phenyl-alanine, serum cholinesterase, urea and uric acid, as well as for phosphatases, transaminases, dehydrogenases, trypsin, histidine, and the like.
• hydrophobed diagnostic agents according to the invention in comparison with all diagnostic agents which have previously been suggested or used for the investigation of whole blood, is the extreme ease with which they are produced and used since sensitive and expensive special coatings or sealings are not necessary.
• EXAMPLE 1 Production of a test strip suitable for the detection of glucose in whole blood.
• Schleicher and Schiill filter paper No. 2312 was successively impregnated with impregnation solutions I and II, dried and cut up into strips of mm. width.
• EXAMPLE 2 Production of a test strip suitable for the detection of galactose in whole blood.
• Impregnation solution I Methylene chloride ad 100.0 ml.
• Schleicher and Schiill filter paper No. 2312 was successively impregnated with impregnation solutions -I and II, dried and cut up into strips.
• Blood containing galactose obtained as described above was applied to the test strips in a manner analogous to that described in Example 1 and the strips further processed as therein set forth.
• the test strips evidence the galactose content of the blood by a blue color reaction, the intensity of which is directly related to the amount of galactose present.
• test strip was produced in exactly the same way as described in Example 1.
• the test strips thereby obtained had the same properties as those of Example 1 and produced the same results.
• Phenyl-alanine test paper Filter paper was impregnated with L-amino acid oxidase, peroxidase and o-tolidine, together with a 0.2 molar phosphate buffer having a pH of 6.5. The dried paper was subsequently impregnated with a solution of 4% paraflin in methylene chloride. The phenyl-alanine test paper obtained in this manner demonstrated, upon the application thereto of blood, containing phenylalanine in a manner analogous to that described in Example 1, the content of L-phenyl-alanine by a blue color reaction which could be quantitatively evaluated by reference to a color table.
• a diagonstic agent for use in the detection of chemical and biochemical components in whole blood comprising an absorbent carrier support impregnated with a composition forming color in response to said chemical component and treated with a hydrophobing agent rendering the impregnated absorbent carrier hydrophobic, wherein said hydrophobing agent is a member selected from the group consisting of natural and synthetic fats, oils, waxes, parafiins, silicones, and mixtures thereof.
• said absorbent carrier support is a member selected from the group consisting of filter paper, asbestos paper, and wood rodlets.
• the method of providing diagnostic agents for use in the detection of chemical and biochemical components in whole blood comprising impregnating an absorbent carrier support with a composition forming color in response to said chemical component and thereafter treating said impregnated carrier support with a hydrophobic agent rendering the impregnated carrier support hydrophobic, wherein said hydrophobic agent is a member selected from the group consisting of natural and synthetic fats, oils, waxes, paraffins, silicones and mixtures thereof.
• composition for forming color in response to said chemical component comprises a mixture of specific reagents and indicators.
• composition for forming color in response to said chemical component comprises a mixture of specific reagents and indicators and wherein said absorbent carrier is first 3,418,083 5 6 impregnated with said specific reagents and thereafter MORRIS O.
• WOLK Primary Examiner. treated with the indicator and hydrophobing agent.

Landscapes

• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Engineering & Computer Science (AREA)
• Immunology (AREA)
• Molecular Biology (AREA)
• Hematology (AREA)
• Physics & Mathematics (AREA)
• Analytical Chemistry (AREA)
• General Health & Medical Sciences (AREA)
• Biochemistry (AREA)
• Organic Chemistry (AREA)
• Wood Science & Technology (AREA)
• General Physics & Mathematics (AREA)
• Biotechnology (AREA)
• Zoology (AREA)
• Proteomics, Peptides & Aminoacids (AREA)
• Microbiology (AREA)
• Urology & Nephrology (AREA)
• Biomedical Technology (AREA)
• Pathology (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Genetics & Genomics (AREA)
• Emergency Medicine (AREA)
• Biophysics (AREA)
• Cell Biology (AREA)
• General Engineering & Computer Science (AREA)
• Food Science & Technology (AREA)
• Medicinal Chemistry (AREA)
• Investigating Or Analysing Biological Materials (AREA)

Applications Claiming Priority (1)

Publications (1)

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ID=6981343

Family Applications (1)

Country Status (13)

Cited By (18)

Families Citing this family (3)

Citations (2)

• 0
  • NL NL129843D patent/NL129843C/xx active
• 1965
  • 1965-05-20 DE DE1965B0082023 patent/DE1598048B2/de active Granted
• 1966
  • 1966-03-17 US US535010A patent/US3418083A/en not_active Expired - Lifetime
  • 1966-05-12 SE SE06543/66A patent/SE326318B/xx unknown
  • 1966-05-16 FI FI661290A patent/FI41879C/fi active
  • 1966-05-17 ES ES0326843A patent/ES326843A1/es not_active Expired
  • 1966-05-17 CH CH714966A patent/CH458793A/de unknown
  • 1966-05-18 NO NO163069A patent/NO116823B/no unknown
  • 1966-05-18 NL NL6606814A patent/NL6606814A/xx unknown
  • 1966-05-18 LU LU51140A patent/LU51140A1/xx unknown
  • 1966-05-18 AT AT474366A patent/AT262511B/de active
  • 1966-05-18 GB GB22112/66A patent/GB1083683A/en not_active Expired
  • 1966-05-18 FR FR62067A patent/FR1484845A/fr not_active Expired
  • 1966-05-20 BE BE681364D patent/BE681364A/xx unknown

Patent Citations (2)

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