Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
  • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
  • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10S435/00—Chemistry: molecular biology and microbiology
  • Y10S435/805—Test papers
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10S436/00—Chemistry: analytical and immunological testing
  • Y10S436/904—Oxidation - reduction indicators

Definitions

• glucose indicator may also be used efficiently in routine urinalyses in hospitals and physicians oflices, in diabetes screening programs, in the differentiation of glucosuria from other meliturias, and the like.
• a urine-sugar test to be of greatest value, must be conveniently rapid, simple enough for any patient to learn with ease, accurate enough to serve the clinician, and sensitive enough to reflect variations in the patients condition.
• the reagent composition must be adequately stable.
• the diagnostic composition according to the basic invention described in the copending application just referred to comprises as essential constituents glucose oxidase, an iodide salt, such as potassium iodide, a molybdate salt, such as sodium molybdate, and a color-forming substance or indicator, such as 2,7-diaminofluorene.
• an iodide salt such as potassium iodide
• a molybdate salt such as sodium molybdate
• a color-forming substance or indicator such as 2,7-diaminofluorene.
• the glucose oxidase (glucose aerodehydrogenase), as is well known, catalyzes the aerobic oxidation of glucose to produce gluconic acid (gluconolactone) and hydrogen peroxide.
• the iodide and molybdate salts are capable of inducing the oxidation of the indicators by hydrogen peroxide formed in the oxidation of glucose.
• Oxidized diamlnofluorene (blue color) The degree of blue color formation is immediately but accurately indicative of the glucose content of the fluid being tested.
• an improved diagnostic composition for the detection of glucose which is not reduced in sensitivity by the presence of ketonic substances may be prepared by the incorporation of a metallic salt of a chelating agent into a formulation which contains a flavoprotein of glucose oxidase, an iodide salt, and a molybdate salt with a colorforming substance oxidizable by hydrogen peroxide in the presence of iodide and molybdate salts.
• potassium iodide and sodium molybdate it is also possible, and therefore contemplated, to employ other equivalent iodide and molybdate salts.
• other iodides such as sodium and ammonium iodides and other molybdates, such as potassium and ammonium molybdates may also be used.
• As a colorforming substance 2,7-diamino-fluorene, o-tolidine, odianisidine, leucoindophenols, etc., may be used.
• pH range may vary between 4 to 7.5, an especially preferred pH is 6.7.
• test device itself may comprise the reagent composition in the form of a tablet, powder or other embodiment
• Embodying the diagnostic composition in the form of test sticks insures ease and simplicity of test procedure.
• Bibulous strips such as filter paper cut into narrow strips
• These strips are then dried in a drying tunnel or in a forced draft oven.
• the test portion of these strips is of a red color. It will be understood that other porous or absorbing materials, such as small sticks of wood, etc., and other methods of applying the impregnating solution and of drying the impregnated sticks may likewise be employed.
• test strips of this example are extraordinarily sensitive when compared to test strips commercially available but, as shown, their sensitivity is somewhat impaired by the presence of ketonic substances present in body fluids being tested.
• Example II the formulation of the tests strips of Example I above was varied slightly by the incorporation into the formula of a heavy metallic salt of a chelating agent. This formulation is set out in detail in Example II as follows:
• Example II The improved reagent strips of Example II were tested against the same urines as set out in Table I above with the following results:
• molybdate salt used is the sodium salt and the iodide salt selected is the potassium salt. It is to be understood, of course, that other salts may be used.
• operable molybdate salts include sodium and potassium.
• Sodium and ammonium iodide may be used as the iodide salts.
• the present invention relates to a diagnostic test for the detection of glucose in body fluids, especially in urine, which is not sensitive to interference by ketonic substances.
• the improved composition of the instant invention consists of a bibulous material which has been impregnated with a composition comprising glucose oxidase, an iodide salt, a molybdate salt, an oxidizable substance which changes color in the presence of hydrogen peroxide and said salts and a metallic salt of a chelating agent.
• the preferred embodiment of the present invention comprises a bibulous material which has been impregnated with a composition consisting of glucose oxidase, potassium iodide, sodium molyb-date, 2,7-diaminofluorene, and an iron salt of ethylene diamine tetraaeetic acid.
• a diagnostic composition for detecting glucose which comprises glucose oxidase, an iodide salt selected from the group consisting of potassium iodide, sodium iodide, and ammonium iodide, a molybdate salt selected from the group. consisting of sodium molybdate, potassium molybdate, and ammonium molybdate, a color-forming substance oxidizable by hydrogen peroxide in the presence of said iodide and molybdate salts, and a metallic salt of a chelating agent selected from the group consisting of the copper, nickel, manganese and iron salts of ethylene diamine tetra-acetic acid.
• a diagnostic composition for detecting glucose which com-prises glucose oxidase, an iodide salt selected from the group consisting of potassium iodide, sodium iodide, and ammonium iodide, a molybdate salt selected from the group consisting of sodium molybdate, potassium molybd'ate, and ammonium molybdate, a color-forming substance selected from the group consisting of 2,7-diaminofluorene, o-tolidine, and dianisidine and mixtures thereof, and a metallic salt of a chel-ating agent selected from the group consisting of the copper, iron, nickel and manganese salts of ethylene diamine tetraacetic acid.
• an iodide salt selected from the group consisting of potassium iodide, sodium iodide, and ammonium iodide
• a molybdate salt selected from the group consisting of sodium molybdate, potassium molybd'ate, and am
• a diagnostic composition for detecting glucose which comprises glucose oxidase, potassium iodide, sodium molybdate, 2,7-diaminoiiuorene, o-tolidine and the ferric salt of ethylene diamine tetraacetic acid.
• a test indicator for detecting glucose which comprises a bibulous material which contains therein the dried residue resulting from deposition on said material, followed by drying, of a liquid comprising glucose oxidase, potassium iodide, sodium molybdate, 2,7-diaminofluorene, o-tolidine, and the ferric salt of ethylene diamine tetraacetic acid.

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• Chemical & Material Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Health & Medical Sciences (AREA)
• Organic Chemistry (AREA)
• Engineering & Computer Science (AREA)
• Zoology (AREA)
• Wood Science & Technology (AREA)
• Proteomics, Peptides & Aminoacids (AREA)
• Analytical Chemistry (AREA)
• Emergency Medicine (AREA)
• Immunology (AREA)
• Microbiology (AREA)
• Molecular Biology (AREA)
• Biophysics (AREA)
• Physics & Mathematics (AREA)
• Biotechnology (AREA)
• Biochemistry (AREA)
• Bioinformatics & Cheminformatics (AREA)
• General Engineering & Computer Science (AREA)
• General Health & Medical Sciences (AREA)
• Genetics & Genomics (AREA)
• Investigating Or Analysing Biological Materials (AREA)
• Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

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Cited By (13)

Citations (10)

• 0
  • BE BE627415D patent/BE627415A/xx unknown
  • NL NL288096D patent/NL288096A/xx unknown
• 1962
  • 1962-01-24 US US168553A patent/US3266868A/en not_active Expired - Lifetime
• 1963
  • 1963-01-23 GB GB2915/63A patent/GB1018306A/en not_active Expired

Patent Citations (10)

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