US3266868A - Diagnostic composition and test indicator - Google Patents
Diagnostic composition and test indicator Download PDFInfo
- Publication number
- US3266868A US3266868A US168553A US16855362A US3266868A US 3266868 A US3266868 A US 3266868A US 168553 A US168553 A US 168553A US 16855362 A US16855362 A US 16855362A US 3266868 A US3266868 A US 3266868A
- Authority
- US
- United States
- Prior art keywords
- glucose
- iodide
- molybdate
- salt
- add
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000012360 testing method Methods 0.000 title claims description 20
- 239000000203 mixture Substances 0.000 title description 26
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims description 29
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 27
- 239000008103 glucose Substances 0.000 claims description 27
- 239000004366 Glucose oxidase Substances 0.000 claims description 13
- 108010015776 Glucose oxidase Proteins 0.000 claims description 13
- 229940116332 glucose oxidase Drugs 0.000 claims description 13
- 235000019420 glucose oxidase Nutrition 0.000 claims description 13
- 150000003839 salts Chemical class 0.000 claims description 13
- 239000011684 sodium molybdate Substances 0.000 claims description 9
- 235000015393 sodium molybdate Nutrition 0.000 claims description 9
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 9
- NUIURNJTPRWVAP-UHFFFAOYSA-N 3,3'-Dimethylbenzidine Chemical compound C1=C(N)C(C)=CC(C=2C=C(C)C(N)=CC=2)=C1 NUIURNJTPRWVAP-UHFFFAOYSA-N 0.000 claims description 7
- SNCJAJRILVFXAE-UHFFFAOYSA-N 9h-fluorene-2,7-diamine Chemical compound NC1=CC=C2C3=CC=C(N)C=C3CC2=C1 SNCJAJRILVFXAE-UHFFFAOYSA-N 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- 229960001484 edetic acid Drugs 0.000 claims description 5
- 235000007715 potassium iodide Nutrition 0.000 claims description 4
- 230000008021 deposition Effects 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 20
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 15
- 239000000243 solution Substances 0.000 description 15
- MEFBJEMVZONFCJ-UHFFFAOYSA-N molybdate Chemical class [O-][Mo]([O-])(=O)=O MEFBJEMVZONFCJ-UHFFFAOYSA-N 0.000 description 13
- 239000000126 substance Substances 0.000 description 10
- 210000002700 urine Anatomy 0.000 description 10
- 239000003153 chemical reaction reagent Substances 0.000 description 9
- 229910052742 iron Inorganic materials 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- XMBWDFGMSWQBCA-UHFFFAOYSA-M iodide Chemical compound [I-] XMBWDFGMSWQBCA-UHFFFAOYSA-M 0.000 description 6
- 230000035945 sensitivity Effects 0.000 description 6
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 description 6
- 229940123150 Chelating agent Drugs 0.000 description 5
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 5
- 239000002738 chelating agent Substances 0.000 description 5
- 229910052802 copper Inorganic materials 0.000 description 5
- 239000010949 copper Substances 0.000 description 5
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 239000011734 sodium Substances 0.000 description 5
- WDJHALXBUFZDSR-UHFFFAOYSA-N Acetoacetic acid Natural products CC(=O)CC(O)=O WDJHALXBUFZDSR-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 239000013522 chelant Substances 0.000 description 4
- WOWBFOBYOAGEEA-UHFFFAOYSA-N diafenthiuron Chemical compound CC(C)C1=C(NC(=S)NC(C)(C)C)C(C(C)C)=CC(OC=2C=CC=CC=2)=C1 WOWBFOBYOAGEEA-UHFFFAOYSA-N 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 230000003647 oxidation Effects 0.000 description 4
- 238000007254 oxidation reaction Methods 0.000 description 4
- 229910052700 potassium Inorganic materials 0.000 description 4
- 239000011591 potassium Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- XZXYQEHISUMZAT-UHFFFAOYSA-N 2-[(2-hydroxy-5-methylphenyl)methyl]-4-methylphenol Chemical compound CC1=CC=C(O)C(CC=2C(=CC=C(C)C=2)O)=C1 XZXYQEHISUMZAT-UHFFFAOYSA-N 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 3
- 229940107816 ammonium iodide Drugs 0.000 description 3
- 235000018660 ammonium molybdate Nutrition 0.000 description 3
- 210000001124 body fluid Anatomy 0.000 description 3
- 239000010839 body fluid Substances 0.000 description 3
- 206010012601 diabetes mellitus Diseases 0.000 description 3
- 230000001771 impaired effect Effects 0.000 description 3
- 229910052759 nickel Inorganic materials 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000011609 ammonium molybdate Substances 0.000 description 2
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 2
- 229940010552 ammonium molybdate Drugs 0.000 description 2
- 239000013060 biological fluid Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000002405 diagnostic procedure Methods 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 150000004694 iodide salts Chemical class 0.000 description 2
- 229910052748 manganese Inorganic materials 0.000 description 2
- 239000011572 manganese Substances 0.000 description 2
- -1 potassium iodide Chemical compound 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 235000009518 sodium iodide Nutrition 0.000 description 2
- QIJNJJZPYXGIQM-UHFFFAOYSA-N 1lambda4,2lambda4-dimolybdacyclopropa-1,2,3-triene Chemical compound [Mo]=C=[Mo] QIJNJJZPYXGIQM-UHFFFAOYSA-N 0.000 description 1
- JRBJSXQPQWSCCF-UHFFFAOYSA-N 3,3'-Dimethoxybenzidine Chemical compound C1=C(N)C(OC)=CC(C=2C=C(OC)C(N)=CC=2)=C1 JRBJSXQPQWSCCF-UHFFFAOYSA-N 0.000 description 1
- SKKKJNPBIGQNEJ-UHFFFAOYSA-N 9h-fluorene-1,9-diamine Chemical compound C1=CC(N)=C2C(N)C3=CC=CC=C3C2=C1 SKKKJNPBIGQNEJ-UHFFFAOYSA-N 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- PHOQVHQSTUBQQK-SQOUGZDYSA-N D-glucono-1,5-lactone Chemical compound OC[C@H]1OC(=O)[C@H](O)[C@@H](O)[C@@H]1O PHOQVHQSTUBQQK-SQOUGZDYSA-N 0.000 description 1
- 102000003983 Flavoproteins Human genes 0.000 description 1
- 108010057573 Flavoproteins Proteins 0.000 description 1
- RGHNJXZEOKUKBD-SQOUGZDYSA-N Gluconic acid Natural products OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 1
- 206010018473 Glycosuria Diseases 0.000 description 1
- 229910021260 NaFe Inorganic materials 0.000 description 1
- 108700020962 Peroxidase Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 239000011358 absorbing material Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000000443 aerosol Substances 0.000 description 1
- UKFWSNCTAHXBQN-UHFFFAOYSA-N ammonium iodide Chemical class [NH4+].[I-] UKFWSNCTAHXBQN-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012992 electron transfer agent Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 235000012209 glucono delta-lactone Nutrition 0.000 description 1
- 229960003681 gluconolactone Drugs 0.000 description 1
- 150000002303 glucose derivatives Chemical class 0.000 description 1
- 230000035780 glucosuria Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 150000002505 iron Chemical class 0.000 description 1
- 159000000014 iron salts Chemical class 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 150000002696 manganese Chemical class 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000012476 oxidizable substance Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 238000006276 transfer reaction Methods 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/805—Test papers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/904—Oxidation - reduction indicators
Definitions
- glucose indicator may also be used efficiently in routine urinalyses in hospitals and physicians oflices, in diabetes screening programs, in the differentiation of glucosuria from other meliturias, and the like.
- a urine-sugar test to be of greatest value, must be conveniently rapid, simple enough for any patient to learn with ease, accurate enough to serve the clinician, and sensitive enough to reflect variations in the patients condition.
- the reagent composition must be adequately stable.
- the diagnostic composition according to the basic invention described in the copending application just referred to comprises as essential constituents glucose oxidase, an iodide salt, such as potassium iodide, a molybdate salt, such as sodium molybdate, and a color-forming substance or indicator, such as 2,7-diaminofluorene.
- an iodide salt such as potassium iodide
- a molybdate salt such as sodium molybdate
- a color-forming substance or indicator such as 2,7-diaminofluorene.
- the glucose oxidase (glucose aerodehydrogenase), as is well known, catalyzes the aerobic oxidation of glucose to produce gluconic acid (gluconolactone) and hydrogen peroxide.
- the iodide and molybdate salts are capable of inducing the oxidation of the indicators by hydrogen peroxide formed in the oxidation of glucose.
- Oxidized diamlnofluorene (blue color) The degree of blue color formation is immediately but accurately indicative of the glucose content of the fluid being tested.
- an improved diagnostic composition for the detection of glucose which is not reduced in sensitivity by the presence of ketonic substances may be prepared by the incorporation of a metallic salt of a chelating agent into a formulation which contains a flavoprotein of glucose oxidase, an iodide salt, and a molybdate salt with a colorforming substance oxidizable by hydrogen peroxide in the presence of iodide and molybdate salts.
- potassium iodide and sodium molybdate it is also possible, and therefore contemplated, to employ other equivalent iodide and molybdate salts.
- other iodides such as sodium and ammonium iodides and other molybdates, such as potassium and ammonium molybdates may also be used.
- As a colorforming substance 2,7-diamino-fluorene, o-tolidine, odianisidine, leucoindophenols, etc., may be used.
- pH range may vary between 4 to 7.5, an especially preferred pH is 6.7.
- test device itself may comprise the reagent composition in the form of a tablet, powder or other embodiment
- Embodying the diagnostic composition in the form of test sticks insures ease and simplicity of test procedure.
- Bibulous strips such as filter paper cut into narrow strips
- These strips are then dried in a drying tunnel or in a forced draft oven.
- the test portion of these strips is of a red color. It will be understood that other porous or absorbing materials, such as small sticks of wood, etc., and other methods of applying the impregnating solution and of drying the impregnated sticks may likewise be employed.
- test strips of this example are extraordinarily sensitive when compared to test strips commercially available but, as shown, their sensitivity is somewhat impaired by the presence of ketonic substances present in body fluids being tested.
- Example II the formulation of the tests strips of Example I above was varied slightly by the incorporation into the formula of a heavy metallic salt of a chelating agent. This formulation is set out in detail in Example II as follows:
- Example II The improved reagent strips of Example II were tested against the same urines as set out in Table I above with the following results:
- molybdate salt used is the sodium salt and the iodide salt selected is the potassium salt. It is to be understood, of course, that other salts may be used.
- operable molybdate salts include sodium and potassium.
- Sodium and ammonium iodide may be used as the iodide salts.
- the present invention relates to a diagnostic test for the detection of glucose in body fluids, especially in urine, which is not sensitive to interference by ketonic substances.
- the improved composition of the instant invention consists of a bibulous material which has been impregnated with a composition comprising glucose oxidase, an iodide salt, a molybdate salt, an oxidizable substance which changes color in the presence of hydrogen peroxide and said salts and a metallic salt of a chelating agent.
- the preferred embodiment of the present invention comprises a bibulous material which has been impregnated with a composition consisting of glucose oxidase, potassium iodide, sodium molyb-date, 2,7-diaminofluorene, and an iron salt of ethylene diamine tetraaeetic acid.
- a diagnostic composition for detecting glucose which comprises glucose oxidase, an iodide salt selected from the group consisting of potassium iodide, sodium iodide, and ammonium iodide, a molybdate salt selected from the group. consisting of sodium molybdate, potassium molybdate, and ammonium molybdate, a color-forming substance oxidizable by hydrogen peroxide in the presence of said iodide and molybdate salts, and a metallic salt of a chelating agent selected from the group consisting of the copper, nickel, manganese and iron salts of ethylene diamine tetra-acetic acid.
- a diagnostic composition for detecting glucose which com-prises glucose oxidase, an iodide salt selected from the group consisting of potassium iodide, sodium iodide, and ammonium iodide, a molybdate salt selected from the group consisting of sodium molybdate, potassium molybd'ate, and ammonium molybdate, a color-forming substance selected from the group consisting of 2,7-diaminofluorene, o-tolidine, and dianisidine and mixtures thereof, and a metallic salt of a chel-ating agent selected from the group consisting of the copper, iron, nickel and manganese salts of ethylene diamine tetraacetic acid.
- an iodide salt selected from the group consisting of potassium iodide, sodium iodide, and ammonium iodide
- a molybdate salt selected from the group consisting of sodium molybdate, potassium molybd'ate, and am
- a diagnostic composition for detecting glucose which comprises glucose oxidase, potassium iodide, sodium molybdate, 2,7-diaminoiiuorene, o-tolidine and the ferric salt of ethylene diamine tetraacetic acid.
- a test indicator for detecting glucose which comprises a bibulous material which contains therein the dried residue resulting from deposition on said material, followed by drying, of a liquid comprising glucose oxidase, potassium iodide, sodium molybdate, 2,7-diaminofluorene, o-tolidine, and the ferric salt of ethylene diamine tetraacetic acid.
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Emergency Medicine (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BE627415D BE627415A (en)) | 1962-01-24 | ||
| NL288096D NL288096A (en)) | 1962-01-24 | ||
| US168553A US3266868A (en) | 1962-01-24 | 1962-01-24 | Diagnostic composition and test indicator |
| FR922431A FR1344835A (fr) | 1962-01-24 | 1963-01-23 | Composition perfectionnée pour doser et déceler le glucose dans les fluides biologiques |
| GB2915/63A GB1018306A (en) | 1962-01-24 | 1963-01-23 | Improved diagnostic composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US168553A US3266868A (en) | 1962-01-24 | 1962-01-24 | Diagnostic composition and test indicator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| US3266868A true US3266868A (en) | 1966-08-16 |
Family
ID=22611978
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US168553A Expired - Lifetime US3266868A (en) | 1962-01-24 | 1962-01-24 | Diagnostic composition and test indicator |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US3266868A (en)) |
| BE (1) | BE627415A (en)) |
| GB (1) | GB1018306A (en)) |
| NL (1) | NL288096A (en)) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3367842A (en) * | 1965-02-17 | 1968-02-06 | Miles Lab | Test composition and device for the detection of galactose in fluids |
| US3411887A (en) * | 1964-06-15 | 1968-11-19 | Miles Lab | Diagnostic composition |
| DE2500689A1 (de) * | 1974-01-09 | 1975-07-10 | Shionogi & Co | Diagnosemittel und diese enthaltende diagnosevorrichtung |
| US4132527A (en) * | 1974-01-09 | 1979-01-02 | Shionogi & Co., Ltd. | Diagnostic compositions, diagnosing instruments, and methods of manufacturing same |
| US4143080A (en) * | 1976-11-25 | 1979-03-06 | The Goodyear Tire & Rubber Company | Method and reagent for the assay of hydroperoxide |
| US4288541A (en) * | 1979-10-15 | 1981-09-08 | Miles Laboratories, Inc. | Ascorbate resistant composition, test device and method for detecting a component in a liquid test sample |
| US4303409A (en) * | 1979-04-24 | 1981-12-01 | Shionogi & Co., Ltd. | Method, composition and test strip for colorimetric analysis of ascorbic acid |
| US4469110A (en) * | 1981-06-25 | 1984-09-04 | Slama Gerard J | Device for causing a pinprick to obtain and to test a drop of blood |
| US4587220A (en) * | 1983-03-28 | 1986-05-06 | Miles Laboratories, Inc. | Ascorbate interference-resistant composition, device and method for the determination of peroxidatively active substances |
| US4658833A (en) * | 1983-03-08 | 1987-04-21 | Stuart James F B | Monitoring of drug levels |
| US6583722B2 (en) | 2000-12-12 | 2003-06-24 | Kimberly-Clark Worldwide, Inc. | Wetness signaling device |
| US6603403B2 (en) | 2000-12-12 | 2003-08-05 | Kimberly-Clark Worldwide, Inc. | Remote, wetness signaling system |
| US9121831B2 (en) | 2011-08-24 | 2015-09-01 | Multisorb Technologies, Inc. | Copper, starch and iodide moisture indicator |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2848308A (en) * | 1955-12-05 | 1958-08-19 | Miles Lab | Composition of matter |
| US2981606A (en) * | 1955-06-20 | 1961-04-25 | Lilly Co Eli | Glucose indicator and method |
| US2982700A (en) * | 1961-05-02 | Diagnostic composition | ||
| US2990338A (en) * | 1959-11-24 | 1961-06-27 | Gibson Jacob John | Composition for determination of glucose in body fluids |
| US2990253A (en) * | 1959-05-21 | 1961-06-27 | Miles Lab | Diagnostic composition |
| US3016292A (en) * | 1959-05-15 | 1962-01-09 | Miles Lab | Diagnostic composition |
| US3042496A (en) * | 1959-03-27 | 1962-07-03 | Miles Lab | Diagnostic composition |
| US3104209A (en) * | 1960-02-02 | 1963-09-17 | Fermco Lab Inc | Composition for determination of glucose |
| US3123443A (en) * | 1960-04-18 | 1964-03-03 | Composition for diagnosing glucose | |
| US3143476A (en) * | 1954-09-15 | 1964-08-04 | Curtis Helene Ind Inc | Cold wave neutralizing composition and process |
-
0
- NL NL288096D patent/NL288096A/xx unknown
- BE BE627415D patent/BE627415A/xx unknown
-
1962
- 1962-01-24 US US168553A patent/US3266868A/en not_active Expired - Lifetime
-
1963
- 1963-01-23 GB GB2915/63A patent/GB1018306A/en not_active Expired
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2982700A (en) * | 1961-05-02 | Diagnostic composition | ||
| US3143476A (en) * | 1954-09-15 | 1964-08-04 | Curtis Helene Ind Inc | Cold wave neutralizing composition and process |
| US2981606A (en) * | 1955-06-20 | 1961-04-25 | Lilly Co Eli | Glucose indicator and method |
| US2848308A (en) * | 1955-12-05 | 1958-08-19 | Miles Lab | Composition of matter |
| US3042496A (en) * | 1959-03-27 | 1962-07-03 | Miles Lab | Diagnostic composition |
| US3016292A (en) * | 1959-05-15 | 1962-01-09 | Miles Lab | Diagnostic composition |
| US2990253A (en) * | 1959-05-21 | 1961-06-27 | Miles Lab | Diagnostic composition |
| US2990338A (en) * | 1959-11-24 | 1961-06-27 | Gibson Jacob John | Composition for determination of glucose in body fluids |
| US3104209A (en) * | 1960-02-02 | 1963-09-17 | Fermco Lab Inc | Composition for determination of glucose |
| US3123443A (en) * | 1960-04-18 | 1964-03-03 | Composition for diagnosing glucose |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3411887A (en) * | 1964-06-15 | 1968-11-19 | Miles Lab | Diagnostic composition |
| US3367842A (en) * | 1965-02-17 | 1968-02-06 | Miles Lab | Test composition and device for the detection of galactose in fluids |
| DE2500689A1 (de) * | 1974-01-09 | 1975-07-10 | Shionogi & Co | Diagnosemittel und diese enthaltende diagnosevorrichtung |
| US4132527A (en) * | 1974-01-09 | 1979-01-02 | Shionogi & Co., Ltd. | Diagnostic compositions, diagnosing instruments, and methods of manufacturing same |
| US4143080A (en) * | 1976-11-25 | 1979-03-06 | The Goodyear Tire & Rubber Company | Method and reagent for the assay of hydroperoxide |
| US4303409A (en) * | 1979-04-24 | 1981-12-01 | Shionogi & Co., Ltd. | Method, composition and test strip for colorimetric analysis of ascorbic acid |
| US4288541A (en) * | 1979-10-15 | 1981-09-08 | Miles Laboratories, Inc. | Ascorbate resistant composition, test device and method for detecting a component in a liquid test sample |
| US4469110A (en) * | 1981-06-25 | 1984-09-04 | Slama Gerard J | Device for causing a pinprick to obtain and to test a drop of blood |
| US4658833A (en) * | 1983-03-08 | 1987-04-21 | Stuart James F B | Monitoring of drug levels |
| US4587220A (en) * | 1983-03-28 | 1986-05-06 | Miles Laboratories, Inc. | Ascorbate interference-resistant composition, device and method for the determination of peroxidatively active substances |
| US6583722B2 (en) | 2000-12-12 | 2003-06-24 | Kimberly-Clark Worldwide, Inc. | Wetness signaling device |
| US6603403B2 (en) | 2000-12-12 | 2003-08-05 | Kimberly-Clark Worldwide, Inc. | Remote, wetness signaling system |
| US9121831B2 (en) | 2011-08-24 | 2015-09-01 | Multisorb Technologies, Inc. | Copper, starch and iodide moisture indicator |
Also Published As
| Publication number | Publication date |
|---|---|
| BE627415A (en)) | |
| NL288096A (en)) | |
| GB1018306A (en) | 1966-01-26 |
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