US3121612A - Composition and method for determination of albumin in fluids, particularly urine - Google Patents
Composition and method for determination of albumin in fluids, particularly urine Download PDFInfo
- Publication number
- US3121612A US3121612A US577671A US57767156A US3121612A US 3121612 A US3121612 A US 3121612A US 577671 A US577671 A US 577671A US 57767156 A US57767156 A US 57767156A US 3121612 A US3121612 A US 3121612A
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- United States
- Prior art keywords
- albumin
- protein
- acid
- liquid
- color
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- 102000009027 Albumins Human genes 0.000 title claims description 24
- 108010088751 Albumins Proteins 0.000 title claims description 24
- 239000000203 mixture Substances 0.000 title claims description 21
- 238000000034 method Methods 0.000 title claims description 11
- 210000002700 urine Anatomy 0.000 title description 13
- 239000012530 fluid Substances 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 claims description 27
- 108090000623 proteins and genes Proteins 0.000 claims description 27
- 239000007788 liquid Substances 0.000 claims description 20
- 238000012360 testing method Methods 0.000 claims description 19
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 13
- 239000006260 foam Substances 0.000 claims description 9
- 239000001569 carbon dioxide Substances 0.000 claims description 6
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 6
- 238000000151 deposition Methods 0.000 claims description 3
- 239000000975 dye Substances 0.000 description 22
- 239000002253 acid Substances 0.000 description 13
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 10
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 8
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 description 6
- 230000001376 precipitating effect Effects 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- WXHLLJAMBQLULT-UHFFFAOYSA-N 2-[[6-[4-(2-hydroxyethyl)piperazin-1-yl]-2-methylpyrimidin-4-yl]amino]-n-(2-methyl-6-sulfanylphenyl)-1,3-thiazole-5-carboxamide;hydrate Chemical compound O.C=1C(N2CCN(CCO)CC2)=NC(C)=NC=1NC(S1)=NC=C1C(=O)NC1=C(C)C=CC=C1S WXHLLJAMBQLULT-UHFFFAOYSA-N 0.000 description 4
- DIZPMCHEQGEION-UHFFFAOYSA-H aluminium sulfate (anhydrous) Chemical compound [Al+3].[Al+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O DIZPMCHEQGEION-UHFFFAOYSA-H 0.000 description 4
- 235000015165 citric acid Nutrition 0.000 description 4
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 description 4
- 238000001556 precipitation Methods 0.000 description 4
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 4
- 235000017557 sodium bicarbonate Nutrition 0.000 description 4
- GCLGEJMYGQKIIW-UHFFFAOYSA-H sodium hexametaphosphate Chemical compound [Na]OP1(=O)OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])O1 GCLGEJMYGQKIIW-UHFFFAOYSA-H 0.000 description 4
- UDEJEOLNSNYQSX-UHFFFAOYSA-J tetrasodium;2,4,6,8-tetraoxido-1,3,5,7,2$l^{5},4$l^{5},6$l^{5},8$l^{5}-tetraoxatetraphosphocane 2,4,6,8-tetraoxide Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P1(=O)OP([O-])(=O)OP([O-])(=O)OP([O-])(=O)O1 UDEJEOLNSNYQSX-UHFFFAOYSA-J 0.000 description 4
- AXDJCCTWPBKUKL-UHFFFAOYSA-N 4-[(4-aminophenyl)-(4-imino-3-methylcyclohexa-2,5-dien-1-ylidene)methyl]aniline;hydron;chloride Chemical compound Cl.C1=CC(=N)C(C)=CC1=C(C=1C=CC(N)=CC=1)C1=CC=C(N)C=C1 AXDJCCTWPBKUKL-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- -1 Calcium sulfate Boric acid Sodium Chemical compound 0.000 description 3
- PLXBWHJQWKZRKG-UHFFFAOYSA-N Resazurin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3[N+]([O-])=C21 PLXBWHJQWKZRKG-UHFFFAOYSA-N 0.000 description 3
- 239000013078 crystal Substances 0.000 description 3
- 229940005740 hexametaphosphate Drugs 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- RLJMLMKIBZAXJO-UHFFFAOYSA-N lead nitrate Chemical compound [O-][N+](=O)O[Pb]O[N+]([O-])=O RLJMLMKIBZAXJO-UHFFFAOYSA-N 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 235000019982 sodium hexametaphosphate Nutrition 0.000 description 3
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 3
- 229960001763 zinc sulfate Drugs 0.000 description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 description 3
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 2
- FRPHFZCDPYBUAU-UHFFFAOYSA-N Bromocresolgreen Chemical compound CC1=C(Br)C(O)=C(Br)C=C1C1(C=2C(=C(Br)C(O)=C(Br)C=2)C)C2=CC=CC=C2S(=O)(=O)O1 FRPHFZCDPYBUAU-UHFFFAOYSA-N 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical group C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical class [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 229910000288 alkali metal carbonate Inorganic materials 0.000 description 2
- 150000008041 alkali metal carbonates Chemical class 0.000 description 2
- WDIHJSXYQDMJHN-UHFFFAOYSA-L barium chloride Chemical compound [Cl-].[Cl-].[Ba+2] WDIHJSXYQDMJHN-UHFFFAOYSA-L 0.000 description 2
- 229910001626 barium chloride Inorganic materials 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229910017604 nitric acid Inorganic materials 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- UJMBCXLDXJUMFB-GLCFPVLVSA-K tartrazine Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)C1=NN(C=2C=CC(=CC=2)S([O-])(=O)=O)C(=O)C1\N=N\C1=CC=C(S([O-])(=O)=O)C=C1 UJMBCXLDXJUMFB-GLCFPVLVSA-K 0.000 description 2
- 229960000943 tartrazine Drugs 0.000 description 2
- 235000012756 tartrazine Nutrition 0.000 description 2
- 239000004149 tartrazine Substances 0.000 description 2
- LIIDWKDFORMMDQ-UHFFFAOYSA-N 2-[[4-(dipropylamino)phenyl]diazenyl]benzoic acid Chemical compound C1=CC(N(CCC)CCC)=CC=C1N=NC1=CC=CC=C1C(O)=O LIIDWKDFORMMDQ-UHFFFAOYSA-N 0.000 description 1
- WCLFKJOZWAMZEL-UHFFFAOYSA-N 3-(1,1-diphenylpropyl)benzene-1,2-diamine Chemical compound C(C)C(C1=C(C(=CC=C1)N)N)(C1=CC=CC=C1)C1=CC=CC=C1 WCLFKJOZWAMZEL-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- GEIOJAPAEZVIIQ-UHFFFAOYSA-N C(CC)N(C1=CC=CC=C1)CCC.C(C=1C(N)=CC=CC1)(=O)O Chemical compound C(CC)N(C1=CC=CC=C1)CCC.C(C=1C(N)=CC=CC1)(=O)O GEIOJAPAEZVIIQ-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical class [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 229910000318 alkali metal phosphate Inorganic materials 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229910052788 barium Inorganic materials 0.000 description 1
- DSAJWYNOEDNPEQ-UHFFFAOYSA-N barium atom Chemical compound [Ba] DSAJWYNOEDNPEQ-UHFFFAOYSA-N 0.000 description 1
- XNQLMPZKLZSICC-UHFFFAOYSA-N bis(1H-pyrazol-5-yl)diazene Chemical compound c1cc(N=Nc2cc[nH]n2)n[nH]1 XNQLMPZKLZSICC-UHFFFAOYSA-N 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 229960001506 brilliant green Drugs 0.000 description 1
- HXCILVUBKWANLN-UHFFFAOYSA-N brilliant green cation Chemical compound C1=CC(N(CC)CC)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](CC)CC)C=C1 HXCILVUBKWANLN-UHFFFAOYSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- VBELMRDAQMYTOU-UHFFFAOYSA-N chembl1093419 Chemical compound OC1=CC(O)=CC=C1C(C(=C1)O)=CC2=C1OC1=CC(=O)C(C=3C(=CC(O)=CC=3)O)=CC1=N2 VBELMRDAQMYTOU-UHFFFAOYSA-N 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- OBRMNDMBJQTZHV-UHFFFAOYSA-N cresol red Chemical compound C1=C(O)C(C)=CC(C2(C3=CC=CC=C3S(=O)(=O)O2)C=2C=C(C)C(O)=CC=2)=C1 OBRMNDMBJQTZHV-UHFFFAOYSA-N 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- FDZZZRQASAIRJF-UHFFFAOYSA-M malachite green Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](C)C)C=C1 FDZZZRQASAIRJF-UHFFFAOYSA-M 0.000 description 1
- 229940107698 malachite green Drugs 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- NYGZLYXAPMMJTE-UHFFFAOYSA-M metanil yellow Chemical compound [Na+].[O-]S(=O)(=O)C1=CC=CC(N=NC=2C=CC(NC=3C=CC=CC=3)=CC=2)=C1 NYGZLYXAPMMJTE-UHFFFAOYSA-M 0.000 description 1
- 229940051142 metanil yellow Drugs 0.000 description 1
- CEQFOVLGLXCDCX-WUKNDPDISA-N methyl red Chemical compound C1=CC(N(C)C)=CC=C1\N=N\C1=CC=CC=C1C(O)=O CEQFOVLGLXCDCX-WUKNDPDISA-N 0.000 description 1
- VXLFYNFOITWQPM-UHFFFAOYSA-N n-phenyl-4-phenyldiazenylaniline Chemical compound C=1C=C(N=NC=2C=CC=CC=2)C=CC=1NC1=CC=CC=C1 VXLFYNFOITWQPM-UHFFFAOYSA-N 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000006920 protein precipitation Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000029219 regulation of pH Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- WXKPPMQZRGORPB-UHFFFAOYSA-M sodium;2-hydroxypropane-1,2,3-tricarboxylic acid;acetate Chemical compound [Na+].CC([O-])=O.OC(=O)CC(O)(C(O)=O)CC(O)=O WXKPPMQZRGORPB-UHFFFAOYSA-M 0.000 description 1
- MLVYOYVMOZFHIU-UHFFFAOYSA-M sodium;4-[(4-anilinophenyl)diazenyl]benzenesulfonate Chemical compound [Na+].C1=CC(S(=O)(=O)[O-])=CC=C1N=NC(C=C1)=CC=C1NC1=CC=CC=C1 MLVYOYVMOZFHIU-UHFFFAOYSA-M 0.000 description 1
- MZSDGDXXBZSFTG-UHFFFAOYSA-M sodium;benzenesulfonate Chemical compound [Na+].[O-]S(=O)(=O)C1=CC=CC=C1 MZSDGDXXBZSFTG-UHFFFAOYSA-M 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- IIACRCGMVDHOTQ-UHFFFAOYSA-N sulfamic acid group Chemical group S(N)(O)(=O)=O IIACRCGMVDHOTQ-UHFFFAOYSA-N 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000000472 traumatic effect Effects 0.000 description 1
- 238000002562 urinalysis Methods 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6827—Total protein determination, e.g. albumin in urine
- G01N33/6839—Total protein determination, e.g. albumin in urine involving dyes, e.g. Coomassie blue, bromcresol green
Definitions
- This invention relates to a composition and to a method of using that composition for the determination of albumin in body fluids such as urine, for example.
- Hellers test which involves placing an amount of concentrated nitric acid in a test tube and stratifying a small amount of urine on the nitric acid. A cloudy ring at the junction of the two liquids indicates a positive specimen.
- Picric acid test which involves placing a measured amount of urine in a test tube and adding an equal amount of saturated picric acid solution. Turbidity of the solution indicates a positive specimen.
- albumin is precipitated either as a ring at the junction of the specimen and the precipitating agent, or as a suspension wherein the specimen is thoroughly mixed with the precipitating reagent.
- composition and method for albumin determinations that is unique in its ease of application.
- the nature of the composition and method of use is such that an unskilled person can conduct the test and can, moreover, readily run mass screening test determination on large groups of urine specimens.
- compositions of that tablet in general includes a protein precipitant comprising a member of one or more of the following groups: (1) a strong, solid, water-soluble acid such as suliosalicylic acid or picric acid, (2) the alkali metal phosphates as are particularly represented by sodium tetrametaphosphate or sodium hexametaphosphate, and (3) metal salts such as lead nitrate, zinc sulfate, barium chloride, aluminum sulfate or aluminum chloride.
- a second ingredient of the present composition comprises, an eliervescent couple consisting of a strong, solid, water-soluble acid such as those listed above as protein prccipitants or maleic, tartaric,
- the third primary ingredient is a dye component consisting of one or a plurality of dyes at least one of which has an atlinity for the protein as precipitated from solution to the extent that it is adsorbed upon the precipitated protein.
- one or more of the dyes constituting the dye component must exhibit a color change from its acid to its basic color within the range of pH increase which the precipitated albumin uniquely exhibits.
- Example IX In all of the foregoing examples, precipitation or" the protein was effected below its iso-electrie point.
- sulfosalicylic acid, picric acid, sodium tetrametaphosphate and sodium hexametaphosphate are examples of chemicals which will precipitate protein below the isoeleetric point, the sodium tetrametaphosphate and hexametaphosphate requiring additional acidity such as is provided by sulfamic or citric acids, for example.
- the reactant mixture should be at a pH no higher than 3 and consequently the dye component must change from the acidic color to the basic color at or below a pH of 3.
- the optimum pH for the precipitation appears to be in the range of 2.0 to 2.5.
- Dyes which possess these desirable or essena color difference inasmuch as a pH of 6 seems to be the tia-l attributes are Brilliant Green (sulfate salt of tetrahighest pH at which the method and composition will ethyldiaminotriphenylmethane), Crystal Violet (hexa- Work when the protein is precipitated above its iso-elecmethylpararosaniline chloride), Cresol Red (o-cresolsu ltric point.
- Metanil Yellow ' (monosodium salt of 4-m- I sulfophenylazodiphenylaniine) and Basic Fuchsine (a Citric acid 5 3-7 mixture of rosaniline and pararosaniline hydrochlorides)
- l-fig are examples of dyes that are suitably adsorbed upon the Sodium bicarbonate. 3 2-5 Sodium citrate 14 11-18 precipitated protein but which give a more difficultly determinable result because of their weak tinctorial effect.
- Example IX the sulfosalicylic acid and the sodium hexametaphosphate, both separately and together, precipitate the protein, the acid providing sufilcient acidity for Q lhhthasthig P i Point hlghhl' thal ⁇ that of t the hexametaphosphate to function.
- Calcium sulfate is used as a n and bm-ic acid as a lubricant to aid tabletting' vescence at the tablet surface, the precipitated protein For precipitating albumin above its iso-electric point, 40 cohl-ehttated and isolated on p of the l P- we have found that lead nitrate, Zinc sulfate, barium chlo- Patehtly, tit the momhht of its P P the albumin ride, aluminum sulfate and aluminum chloride exemp ify P Suthvieht Carbon thOXtdc to cause the albumin the water-sol-ublesalts of metals that willfunction in'this to fish to the Surface and remain conversely in role.
- Yellow No. 1 the color changes from violet to yellow.
- the change will be from its acid color to its basic color in positive specimens within the capacity and range of pH increase effected by the ggg g g -g g g g g precipitated protein as has been earlier discussed.
- a method for the determination of albumin in The foregoing are illustrative examples using Bromcresol Green (tetrabromo m cresolsulfon -phthalein), liquid on a tableted mixture of a protein precipitant, a Resazurin (Diazoresorcinol), Lacmoid (heptahydroxy- 7 dye having an affinity for the precipitated protein and a liquid which comprises depositing a test portion of the which exhibits a color-change Within the variation of pH effected by the precipitated protein, and an effervescent couple effective When moistened by said liquid to release carbon dioxide, said carbon dioxide forming with said liquid a foam on the surface of said tablet, said foam including the dyed albumin precipitated from said liquid.
- a method for determining albumin in a liquid the step which comprises precipitating albumin from said liquid by depositing a test portion of the liquid on a tablet comprising a mixture of a protein precipitant, a dye having an aflinity for the precipitated protein and which exhibits a color change Within the variation of pH effected by the precipitated protein, and an effervescent couple eifective when moistened by said liquid to release carbon dioxide, whereby said carbon dioxide forms a foam with said liquid on the surface of said tablet, said foam including dyed precipitated albumin.
- a composition for determining the presence of albumin in liquids comprising a tableted mixture of a protein precipitant selected from the group of metal salts consisting of lead nitrate, zinc sulfate, barium chloride, aluminum sulfate and aluminum chloride, a dye having an afiinity for the precipitated protein, said dye exhib-it ing a color change within the range of pH increase effected by such precipitated protein, and an eflervescent couple consistin essentially of a strong Water-soluble onganic acid and a member of the group consisting of alkali metal carbonates and bicarbonates.
- a protein precipitant selected from the group of metal salts consisting of lead nitrate, zinc sulfate, barium chloride, aluminum sulfate and aluminum chloride
- a dye having an afiinity for the precipitated protein said dye exhib-it ing a color change within the range of pH increase effected by such precipitated protein
- Van Gool Chem. Weekbl, 1950, pp. 7047.
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT569423D IT569423A (cs) | 1956-04-12 | ||
| US577671A US3121612A (en) | 1956-04-12 | 1956-04-12 | Composition and method for determination of albumin in fluids, particularly urine |
| GB33679/56A GB799626A (en) | 1956-04-12 | 1956-11-05 | Method for determination of albumin in fluids, particularly urine |
| CH357890D CH357890A (de) | 1956-04-12 | 1957-03-22 | Mittel zum Nachweis von Albumin |
| FR1173427D FR1173427A (fr) | 1956-04-12 | 1957-03-29 | Procédé de détermination de l'albumine dans l'urine |
| DEM33875A DE1197250B (de) | 1956-04-12 | 1957-04-11 | Diagnostische Tablette zur Bestimmung von Albumin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US577671A US3121612A (en) | 1956-04-12 | 1956-04-12 | Composition and method for determination of albumin in fluids, particularly urine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| US3121612A true US3121612A (en) | 1964-02-18 |
Family
ID=24309670
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US577671A Expired - Lifetime US3121612A (en) | 1956-04-12 | 1956-04-12 | Composition and method for determination of albumin in fluids, particularly urine |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US3121612A (cs) |
| CH (1) | CH357890A (cs) |
| DE (1) | DE1197250B (cs) |
| FR (1) | FR1173427A (cs) |
| GB (1) | GB799626A (cs) |
| IT (1) | IT569423A (cs) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3607081A (en) * | 1969-08-11 | 1971-09-21 | Dow Chemical Co | Reagent for determination of globulin |
| US5194390A (en) * | 1988-07-05 | 1993-03-16 | Miles Inc. | Composition for the assay of albumin |
| US20090233321A1 (en) * | 2008-03-11 | 2009-09-17 | Urobiologics Llc | Reducing/Oxidizing Activity of Maternal Urine As Indicator of Fetal Gender Related Characteristics |
| US10018625B2 (en) | 2008-03-11 | 2018-07-10 | Urobiologics Llc | Use of female mammal's urine for determination of fetal gender related characteristics |
| US10996218B2 (en) | 2008-03-11 | 2021-05-04 | Ournextbaby Llc | Methods for chemotaxis / redox driven separation of X and Y chromosome bearing sperm and their insemination in gender specific menstrual cycles |
| CN112881382A (zh) * | 2021-01-15 | 2021-06-01 | 大自然生物集团有限公司 | 一种食品添加剂柠檬酸钠中硫酸盐含量的测定方法 |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3438737A (en) * | 1965-10-01 | 1969-04-15 | Miles Lab | Protein test composition,device and method |
| JPWO2002046755A1 (ja) * | 2000-12-04 | 2004-04-08 | 松下電器産業株式会社 | 溶液濃度計測方法および尿検査方法 |
| DE102005001362A1 (de) * | 2005-01-11 | 2006-07-20 | Protagen Ag | Verfahren und Vorrichtung zur Quantifizierung von Proteinen |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2171961A (en) * | 1938-08-19 | 1939-09-05 | Lilly Co Eli | Blood-sugar test |
| US2281758A (en) * | 1939-01-31 | 1942-05-05 | Denver Chemical Mfg Company | Dry reagent for testing |
| US2331573A (en) * | 1941-07-28 | 1943-10-12 | Abraham G Sheftel | Test for sulphanilyl compounds |
| US2387244A (en) * | 1942-06-19 | 1945-10-23 | Miles Lab | Tablet and method of dissolving same |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT68346B (de) * | 1913-06-19 | 1915-04-10 | Hans Gallus Dr Pleschner | Einrichtung zur kolorimetrischen Eiweißbestimmung. |
| US2314336A (en) * | 1940-07-03 | 1943-03-23 | Raymond H Goodale | Method and means for simultaneously testing and filtering solutions |
| GB708996A (en) * | 1954-04-15 | 1954-05-12 | Miles Lab | Improvements in or relating to diagnostic compositions |
-
0
- IT IT569423D patent/IT569423A/it unknown
-
1956
- 1956-04-12 US US577671A patent/US3121612A/en not_active Expired - Lifetime
- 1956-11-05 GB GB33679/56A patent/GB799626A/en not_active Expired
-
1957
- 1957-03-22 CH CH357890D patent/CH357890A/de unknown
- 1957-03-29 FR FR1173427D patent/FR1173427A/fr not_active Expired
- 1957-04-11 DE DEM33875A patent/DE1197250B/de active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2171961A (en) * | 1938-08-19 | 1939-09-05 | Lilly Co Eli | Blood-sugar test |
| US2281758A (en) * | 1939-01-31 | 1942-05-05 | Denver Chemical Mfg Company | Dry reagent for testing |
| US2331573A (en) * | 1941-07-28 | 1943-10-12 | Abraham G Sheftel | Test for sulphanilyl compounds |
| US2387244A (en) * | 1942-06-19 | 1945-10-23 | Miles Lab | Tablet and method of dissolving same |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3607081A (en) * | 1969-08-11 | 1971-09-21 | Dow Chemical Co | Reagent for determination of globulin |
| US5194390A (en) * | 1988-07-05 | 1993-03-16 | Miles Inc. | Composition for the assay of albumin |
| US20090233321A1 (en) * | 2008-03-11 | 2009-09-17 | Urobiologics Llc | Reducing/Oxidizing Activity of Maternal Urine As Indicator of Fetal Gender Related Characteristics |
| US9057720B2 (en) * | 2008-03-11 | 2015-06-16 | Urobiologics Llc | Reducing/oxidizing activity of maternal urine as indicator of fetal gender related characteristics |
| US10018625B2 (en) | 2008-03-11 | 2018-07-10 | Urobiologics Llc | Use of female mammal's urine for determination of fetal gender related characteristics |
| US10996218B2 (en) | 2008-03-11 | 2021-05-04 | Ournextbaby Llc | Methods for chemotaxis / redox driven separation of X and Y chromosome bearing sperm and their insemination in gender specific menstrual cycles |
| CN112881382A (zh) * | 2021-01-15 | 2021-06-01 | 大自然生物集团有限公司 | 一种食品添加剂柠檬酸钠中硫酸盐含量的测定方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| GB799626A (en) | 1958-08-13 |
| IT569423A (cs) | |
| DE1197250B (de) | 1965-07-22 |
| FR1173427A (fr) | 1959-02-25 |
| CH357890A (de) | 1961-10-31 |
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