US3085717A - Automatic pipette - Google Patents

Automatic pipette Download PDF

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Publication number
US3085717A
US3085717A US187278A US18727862A US3085717A US 3085717 A US3085717 A US 3085717A US 187278 A US187278 A US 187278A US 18727862 A US18727862 A US 18727862A US 3085717 A US3085717 A US 3085717A
Authority
US
United States
Prior art keywords
syphon
liquid
arrangement
tube
end portion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US187278A
Other languages
English (en)
Inventor
Anscherlik Arnost
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vyzkumny Ustav Energeticky
VYZKUMNY USTAV ENERGETICKY PRAGUE
Original Assignee
VYZKUMNY USTAV ENERGETICKY PRAGUE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by VYZKUMNY USTAV ENERGETICKY PRAGUE filed Critical VYZKUMNY USTAV ENERGETICKY PRAGUE
Application granted granted Critical
Publication of US3085717A publication Critical patent/US3085717A/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J4/00—Feed or outlet devices; Feed or outlet control devices
    • B01J4/02—Feed or outlet devices; Feed or outlet control devices for feeding measured, i.e. prescribed quantities of reagents
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04F—PUMPING OF FLUID BY DIRECT CONTACT OF ANOTHER FLUID OR BY USING INERTIA OF FLUID TO BE PUMPED; SIPHONS
    • F04F1/00—Pumps using positively or negatively pressurised fluid medium acting directly on the liquid to be pumped
    • F04F1/06—Pumps using positively or negatively pressurised fluid medium acting directly on the liquid to be pumped the fluid medium acting on the surface of the liquid to be pumped
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04F—PUMPING OF FLUID BY DIRECT CONTACT OF ANOTHER FLUID OR BY USING INERTIA OF FLUID TO BE PUMPED; SIPHONS
    • F04F10/00—Siphons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
    • G01F11/00—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1002—Reagent dispensers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers

Definitions

  • This invention is more specifically concerned with an automatic pipette capable of discharging a predetermined amount of a liquid and of refilling itself from a storage vessel by gravity responsive to a pneumatic signal.
  • An important object of the invention is the provision of such an automatic pipette in which the measurement and discharge of relatively large amounts of liquid is triggered by a very brief and weak pneumatic signal.
  • Another object is the provision of an automatic pipette in which the duration of the pneumatic signal may be very much shorter than the time required for discharging the measured liquid from the volumetric vessel of the pipette.
  • FIGURE is an elevational sectional view of a pipette arrangement according to this invention.
  • a pipette including a bulb 1, and S-shaped syphon tube 11 communicating with the bottom of the bulb 1 and having a bight portion 12 substantially higher than the bulb 1 and a pressure tube 6 connected to the top of the bulb 1.
  • the pressure tube is upwardly arched above the level of the syphon portion 12, and connects the bulb 1 with a pneumatic pulse generating pump 4.
  • the pump is a vertically disposed tube connected to a source of fluid under pressure in a conventional manner not further illustrated for passage of fluid through tube 4 in the direction of the arrow 41.
  • a constriction 42 in the tube 4 generates positive air pressure in the tube 4 ahead of the constriction 42 in the direction of the arrow 41 when fluid passes through the tube 4.
  • a storage tank 3 contains a body of the liquid 31 which is to be dispensed by the pipette. It is provided with known means for maintaining the liquid 31 at a constant level 33, such constant level means being represented by a float 32 but not otherwise illustrated.
  • the float is connected with means for admitting additional liquid 31 to the tank 3 when the liquid drops below the desired level 33.
  • Contacts closed by downward movement of the float from the illustrated position, and a solenoid valve in series circuit with the contacts and a source of current are one well-known example of a constant level device which will admit liquid from a reserve supply at a higher level to the tank 3. It is preferred, however, to arrange the aforementioned contacts in the actuating circuit of a pump P which raises the liquid from a dump to the tank when the float 32 drops.
  • the tank 3 communicates with a substantially closed transfer vessel 8 through a narrow pipe 7.
  • An air vent tube 81 connects the top of the vessel 8 with the atmosphere.
  • An approximately U-shaped connecting tube 2 connects a portion of the vent tube 81 closely adjacent the top of the vessel 8 with a portion of the pressure tube 6 closely adjacent the top of the bulb 1.
  • An S-shaped syphon tube 82 communicates with the bottom of the transfer vessel 8 and has an upper bight portion 83 which "ice is slightly above the common normal liquid level 33 in the tank 3, the vent tube 81, the pressure tube 6, and the syphon tube 11.
  • the syphon tubes 11 and 82 have respective terminal discharge orifices in their free end portions 13 and 84 which communicate with the atmosphere and are located above a receiving vessel 5 and a dump vessel 9 respec tively.
  • the receiving vessel 5 has an outlet 51 which may lead to a reaction chamber where the liquid 31 may be reacted with another liquid in an automatic titration process or for other purposes which will readily suggest themselves to those skilled in the art.
  • the liquid discharged from the dump vessel 9 through an outlet 91 may be returned to the tank 3 through the afore-mentioned pump P the operation of which is controlled by the float 32.
  • the vessels 5 and 9 have been conventionally illustrated, and their shapes and sizes may be modified to suit specific conditions.
  • the bulb 1 and syphon tube 11 together with a straight portion 61 of the pressure tube 6 contiguously adjacent the bulb 1 constitute a first tubular syphon arrangement having an upper orifice above the level of the bight portion 12 where the afore-mentioned portion 61 of the pressure tube 6 is integrally joined to the remainder of the pressure tube, and a lower terminal orifice in the end portion 13 below the level of the lower bight portion 13 of the syphon tube 11.
  • the transfer vessel 8 together with the event tube 81 and the syphon tube 82 constitutes a second tubular syphon arrangement having an upper terminal orifice in the vent tube 81 above the level of the bight portion 83, and a lower terminal orifice in the end portion 84 of the syphon tube 82, below the level of the lower bight portion 85 of the syphon tube 82.
  • the normal liquid level 33 in the tank 3 in the illustrated non-operative position of the apparatus is thus slightly below the upper bight portions 12 and 83, and above the connecting tube 2.
  • the feed pipe 7 which is narrower than any other conduit in the two syphon arrangements directly communicates with the transfer vessel 8 below the level of the junction between the connecting tube 2 and the vent tube 81.
  • a fluid passing through the tube 4 in the direction of the arrow 41 forces air under a pressure higher than atmospheric pressure into the pressure tube 6. This pressure lowers the liquid level in the straight pressure tube portion 61 and the bulb 1, and forces liquid to flow through the connecting tube 2, thereby briefly raising the liquid level in the vent tube 81.
  • the pressure rise in the tube 6 must be suflicient to drive liquid from the first syphon arrangement through the bight portion 12. The remainder of the liquid will then be automatically syphoned oif into the receiving vessel 5.
  • the pressure pulse in the tube 6 also must be sufiicient to raise the liquid level in the vent tube 8 1 above the bight portion 83. Since the vent tube 81 and the bight portion 83 communicate, a corresponding liquid level rise Will occur in the bight portion 83, and the contents of the second syphon arrangement are drained into the dump vessel 9 at a rate determined by the dimensions of the second syphon arrangement.
  • the apparatus completes its operating cycle regardless of the length of time during which the pressure pulse is effective. Return to the starting condition illustrated, however, is predicated on interruption of the gas flow from the tube 4. While the bight portions 12 and 83 have been shown for the sake of clarity to be elevated beyond the normal liquid level by a distance greater than the internal diameter of the syphon tubes 11, 82, this distance may be chosen to be extremely small if very short or weak applications of pressure through the tube 6 are desired.
  • the apparatus illustrated has the advantage of triggering release of the liquid 32 in a measured amount whenever another liquid or gaseous reagent or sample is passed through the tube 4 into a reaction zone communicating with the vessel 5, but brief pressure pulses of other origin may be employed to initiate liquid discharge.
  • a small bellows arrangement actuated by depressing a button supplies sufficient gas under pressure to trigger the automatic pipette, and other modification of the pressure fluid source constituted by the tube 4 and the tube 6 can readily be devised following the above teachings.
  • the enlarged bore portions of the syphon arrangements constituted by bulb 1 and the transfer vessel 8 are convenient for holding relatively large bodies of liquid 32 in a small space, but both syphon arrangements may consist of tubing of uniform bore without altering the basic mode of operation of the apparatus. Other dimensional relationships may equally be altered, but it is important that the effective flow section of the pipe 7 be smaller than the effective flow section of the second syphon arrangement to prevent replenishment of liquid from the tank 3 at a rate similar or equal to the rate of discharge from the orifice in the end portion 84.
  • the bulb 1 thus is shut off from the liquid supply in the tank 3 while it is drained into the receiving vessel 5.
  • the amounts of liquid discharged from the first syphon arrangement are precisely uniform as long as the liquid level in the tank 3 is maintained constant.
  • An automatic pipette comprising, in combination:
  • each of said arrangements being elongated and substantially S- shaped, each arrangement including in longitudinal sequence an upper vertically extending end portion, a lower bight portion, an upper bight portion, and a lower vertically extending end portion, said end portions having respective terminal orifices;
  • a feed conduit communicating with said tank means and with a portion of said second syphon arrangement intermediate said part thereof and said upper bight portion thereof for passage of said liquid therethrough by gravity from said tank means to said second syphon arrangement at a predetermined rate when said intermediate portion is empty of liquid;
  • said second syphon arrangement being dimensioned for syphoning flow of liquid from the terminal orifice of the lower end portion thereof at a rate greater than said predetermined rate.
  • liquid level maintaining means include means responsive to a lowering of said level for returning liquid discharged from the lower end portion of said second syphon arrangement to said tank means.

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  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Mechanical Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Fluid Mechanics (AREA)
  • Jet Pumps And Other Pumps (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
US187278A 1961-04-24 1962-04-13 Automatic pipette Expired - Lifetime US3085717A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CS250961 1961-04-24

Publications (1)

Publication Number Publication Date
US3085717A true US3085717A (en) 1963-04-16

Family

ID=5362435

Family Applications (1)

Application Number Title Priority Date Filing Date
US187278A Expired - Lifetime US3085717A (en) 1961-04-24 1962-04-13 Automatic pipette

Country Status (6)

Country Link
US (1) US3085717A (da)
BE (1) BE614192A (da)
CH (1) CH392936A (da)
DE (1) DE1219907B (da)
GB (1) GB992495A (da)
NL (2) NL124294C (da)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3182865A (en) * 1963-03-19 1965-05-11 Vyzk Ustav Energeticky Refilling arrangement for an automatic burette
US3259462A (en) * 1961-09-04 1966-07-05 Vyzk Ustar Energeticky Automatic analyzing device and method
US3649218A (en) * 1968-05-08 1972-03-14 Coulter Electronics Method for preparing metered liquid sample dilutions
US3771366A (en) * 1971-03-26 1973-11-13 Aga Ab Device for collection and measurement of liquid volumes
US3901653A (en) * 1973-07-19 1975-08-26 Beckman Instruments Inc Liquid sampling device
US3923464A (en) * 1972-09-25 1975-12-02 Leco Corp Combustion apparatus for analytical instruments
US4002269A (en) * 1974-08-16 1977-01-11 Technicon Instruments Corporation Liquid proportioning system in a liquid sample analyzer
EP0112862A1 (en) * 1982-06-18 1984-07-11 Richter Gedeon Vegyeszet METHOD AND DEVICE FOR CARRYING OUT ROUTINE ANALYZES, FOR EXAMPLE POLAROGRAPHIC OR SPECTROPHOTOMETRIC ANALYZES.
US4630753A (en) * 1983-06-07 1986-12-23 Anscherlik A Device for dispensing doses of liquid
US7189365B1 (en) * 1999-03-04 2007-03-13 Riken Liquid treating equipment including a storage vessel and a discharge vessel

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2842326C2 (de) * 1978-09-28 1982-06-09 CKD Dukla, N.P., 18606 Praha Selbsttätiger Flüssigkeitsanalysator
CN103861370B (zh) * 2014-04-03 2015-09-02 江阴圆方机械制造有限公司 具有带径向狭长槽的连接部和滚珠推力轴承的给液装置
CN103861350B (zh) * 2014-04-03 2015-09-02 江阴圆方机械制造有限公司 具有带电镀涂层中心轴及滚珠推力轴承的给液装置
CN103861352B (zh) * 2014-04-03 2015-09-09 江阴圆方机械制造有限公司 具有滚珠推力轴承和缓冲式限位管路段的给液装置
CN103933771B (zh) * 2014-04-03 2015-08-05 徐同林 具有分层结构密封条和滚珠推力轴承的液体给出装置

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2788157A (en) * 1952-07-16 1957-04-09 Gen Electric Fluid dispensing device

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE554660A (da) *

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2788157A (en) * 1952-07-16 1957-04-09 Gen Electric Fluid dispensing device

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3259462A (en) * 1961-09-04 1966-07-05 Vyzk Ustar Energeticky Automatic analyzing device and method
US3182865A (en) * 1963-03-19 1965-05-11 Vyzk Ustav Energeticky Refilling arrangement for an automatic burette
US3649218A (en) * 1968-05-08 1972-03-14 Coulter Electronics Method for preparing metered liquid sample dilutions
US3771366A (en) * 1971-03-26 1973-11-13 Aga Ab Device for collection and measurement of liquid volumes
US3923464A (en) * 1972-09-25 1975-12-02 Leco Corp Combustion apparatus for analytical instruments
US3901653A (en) * 1973-07-19 1975-08-26 Beckman Instruments Inc Liquid sampling device
US4002269A (en) * 1974-08-16 1977-01-11 Technicon Instruments Corporation Liquid proportioning system in a liquid sample analyzer
EP0112862A1 (en) * 1982-06-18 1984-07-11 Richter Gedeon Vegyeszet METHOD AND DEVICE FOR CARRYING OUT ROUTINE ANALYZES, FOR EXAMPLE POLAROGRAPHIC OR SPECTROPHOTOMETRIC ANALYZES.
EP0112862A4 (en) * 1982-06-18 1984-09-11 Richter Gedeon Vegyeszet METHOD AND APPARATUS FOR PERFORMING ROUTINE ANALYSIS SUCH AS POLAROGRAPHIC OR SPECTROPHOTOMETRIC ANALYSIS.
US4630753A (en) * 1983-06-07 1986-12-23 Anscherlik A Device for dispensing doses of liquid
US7189365B1 (en) * 1999-03-04 2007-03-13 Riken Liquid treating equipment including a storage vessel and a discharge vessel

Also Published As

Publication number Publication date
GB992495A (en) 1965-05-19
CH392936A (de) 1965-05-31
BE614192A (fr) 1962-06-18
NL276916A (da)
DE1219907B (de) 1966-06-30
NL124294C (da)

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