Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
  • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
  • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10S435/00—Chemistry: molecular biology and microbiology
  • Y10S435/805—Test papers
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
  • Y10T436/00—Chemistry: analytical and immunological testing
  • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
  • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
  • Y10T436/143333—Saccharide [e.g., DNA, etc.]
  • Y10T436/144444—Glucose

Definitions

• This invention relates toa new and improved diagnostic 'guided in this regard by a regular check on urine glucose.
• this glucose indicator may also be used efficiently inroutine urinalyses in hospitals and physicians offices, in diabetes detection screening programs, in the dilferentiation of glucosuria from other meliturias, and the like.
• Glucose indicators in stick form present-ly' on the market depend for the detection of hydrogen peroxide on the action of enzymes,-s uch asperoxidases.
• the glucose indicator of the present invention makes use of a non-enzymatic agent which permits a more accurately controllable and more definite composition. A very sensitive test for hydrogen peroxide and,faccordingly, glucose is'thus made possible.
• a diagnostic composition according to the present'im vention comprises as essential constituents glucose oxidase, an iodide salt, such as potassium iodide, a molybdate salt, such as sodium molybdate, and a color-forming substance or indicator, such as 2,7-diaminofluorene.
• an iodide salt such as potassium iodide
• a molybdate salt such as sodium molybdate
• a color-forming substance or indicator such as 2,7-diaminofluorene.
• the glucose oxidase (glucose aerodehydrogenase), as is well known, catalyzes the aerobic oxidation of glucose to produce gluconic acid (gluconolactone) and hydrogen peroxide.
• the iodide and molybdate salts are capable of inducing the oxidation of indicators by hydrogen peroxide formed in the oxidation of glucose.
• the latter reaction may be represented by the following schematic equation: a
• Oxidized diar'ninofiuorene blue color
• potassium iodide and sodium molybdate it is also possible, and therefore contemplated, to employ other equivalent iodide and molybdatelsalts.
• other iodides such as sodium and ammonium iodides and other molybdates, such as potassium 'and ammonium molybdates may also be used.
• i2,7-diaminofluorene o-tolidine, o-dianisidine, leucoindophenols, etc. would be satisfactory indicators.
• additives are incorporated in the reagent composition as suitable protective, thickening, wetting, suspending agents and the like as well as inert dyes to impart thereto a uniformrcolor background.
• test device itself may comprise the reagent composition in the form of a'tablet, powder or other embodiment
• reagent composition in the form of a'tablet, powder or other embodiment
• Embodying the diagnostic composition in 'the form of test sticks insures ease and simplicity. of test procedure.
• phosphate butter of pH 6.5 prepared by dissolving 43.55 gm. of dibasic potassium phosphate (K HPO and 39.0 gm. of monobasic sodium phosphate (NaH PO;.H O) in water and bringing. to a volume of 500 ml.) are added to solution No. 4 with constant stirring.
• Bibulous strips such as filter paper cut into narrow strips
• These strips are then dried in a. drying tunnel or in aforced draft oven.
• the test portion of these strips is of a red color. It will be under stood that other porous or absorbing materials, 'such as small sticks of wood, etc. and other methods of applying the impregnating solution and of drying the impregnated sticks may likewise be employed.
• Test strips are then prepared in accordance with the procedure recited in Example I.
• gelatin-dye solution prepared by dis- (2) 1 gm. of KI, 3 gm. of glycine and 0.5 gm. of Na MoO .2H O are dissolved in 20 ml. of hot water. 10 ml. of 4% gelatin-dye solution is added to this solution.
• the gelatin-dye solution is prepared as described in Example I. v
• cation exchange cellulose has a two-fold purpose: it maintains the indicator in suspension so as to prevent it from precipitating, and it serves as a buffer for the reagent composition.
• These functions in the embodiments of Examples I and II are performed, respectively, by polyvinyl alcohol and the buffer system.
• this invention pertains to a diagnostic test for the detection of glucose in body fluids, especially in urine, consisting of a bibulous strip or stick that has been impregnated with a composition comprising glucose ox1- dase, potassium iodide, sodium molybdate and 2,7-diaminofluorene which is oxidizable by hydrogen peroxide formed in the glucose oxidase-catalyzed decomposition of glucose in the presence of said iodide and molybdate salts.
• a diagnostic composition for detecting glucose which comprises glucoseoxidase, an iodide salt selected from the group consisting of potassium iodide, sodium iodide, and ammonium iodide, a molybdate salt for intensifying the action of the iodide and selected from the group consisting of sodium molybdate, potassium molybdate, and ammonium molybdate and a color-forming substance oxidizable by hydrogen peroxide in the presence of said iodide and molybdate salts.
• an iodide salt selected from the group consisting of potassium iodide, sodium iodide, and ammonium iodide
• a molybdate salt for intensifying the action of the iodide selected from the group consisting of sodium molybdate, potassium molybdate, and ammonium molybdate and a color-forming substance oxidizable by hydrogen peroxide in the presence of said iodide and molybdate
• a diagnostic composition for detecting glucose which comprises glucose oxidase, an iodide salt selected from the group consisting of potassium iodide, sodium iodide,
• a diagnostic composition for detecting glucose which comprises glucose oxidase, potassium iodide, sodium molybdate and 2,7-diaminofluorene.
• a diagnostic composition for detecting glucose which comprises:
• a test indicator for detecting glucose which comprises a bi-bulous material which contains therein the dried residue resulting from deposition on said material, followed by drying, of a liquid comprising glucose oxidase, potassium iodide, sodium molybdate and 2,7-diamino-' fiuorene.
• a test indicator for detecting glucose which comprises a bibulous strip of paper which contains therein the diagnostic composition of claim 4.
• ammonium molybdate and a color-forming substance se-

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• Chemical & Material Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Health & Medical Sciences (AREA)
• Organic Chemistry (AREA)
• Engineering & Computer Science (AREA)
• Zoology (AREA)
• Wood Science & Technology (AREA)
• Proteomics, Peptides & Aminoacids (AREA)
• Analytical Chemistry (AREA)
• Emergency Medicine (AREA)
• Immunology (AREA)
• Microbiology (AREA)
• Molecular Biology (AREA)
• Biophysics (AREA)
• Physics & Mathematics (AREA)
• Biotechnology (AREA)
• Biochemistry (AREA)
• Bioinformatics & Cheminformatics (AREA)
• General Engineering & Computer Science (AREA)
• General Health & Medical Sciences (AREA)
• Genetics & Genomics (AREA)
• Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
• Investigating Or Analysing Biological Materials (AREA)

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Cited By (14)

Citations (2)

Family Cites Families (1)

• 0
  • NL NL251599D patent/NL251599A/xx unknown
  • BE BE590824D patent/BE590824A/xx unknown
  • IT IT630626D patent/IT630626A/it unknown
• 1959
  • 1959-05-15 US US813336A patent/US3016292A/en not_active Expired - Lifetime
• 1960
  • 1960-04-22 GB GB14249/60A patent/GB883744A/en not_active Expired
  • 1960-05-04 DE DEM45193A patent/DE1284124B/de active Pending
  • 1960-05-05 CH CH511860A patent/CH392109A/de unknown

Patent Citations (2)

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