GB784548A - Improvements in or relating to glucose indicator - Google Patents
Improvements in or relating to glucose indicatorInfo
- Publication number
- GB784548A GB784548A GB1971155A GB1971155A GB784548A GB 784548 A GB784548 A GB 784548A GB 1971155 A GB1971155 A GB 1971155A GB 1971155 A GB1971155 A GB 1971155A GB 784548 A GB784548 A GB 784548A
- Authority
- GB
- United Kingdom
- Prior art keywords
- glucose
- enzyme
- indicator
- peroxidase
- ortho
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
Abstract
An indicator composition for detecting or estimating glucose in industrial or body fluids, e.g. urine or blood, comprises an enzyme capable of catalysing a reaction of glucose, and a substance producing a colour change in the presence of a product of the reaction. A suitable enzyme is glucose oxidase of the flavoprotein type, obtained from moulds; glucose reacts with the oxygen of the air in the presence of this enzyme to produce gluconic acid and reduced flavin; the latter is oxidized to flavin, hydrogen peroxide being formed. The indicator may include a substance which changes colour on oxidation or reduction by hydrogen peroxide; such substances are potassium iodide, which may be used alone or together with starch, ortho-phenylene diamine, alcoholic gum guaiac, or o-tolidine; lead iodide or sulphide; or a mixture of a ferrous salt and tannic, gallic, or pyrogallic acid. Alternatively substances which change colour on reduction by reduced flavin may be used; examples are methylene blue, thionine, sodium 2:6-dichloro-benzenone indophenol, sodium 2:6-dibromo-benzene indophenol, and brilliant cresyl blue; in this case the enzyme catalase should preferably be present to destroy the hydrogen peroxide formed. Another suitable enzyme for the reaction of glucose is glucose dehydrogenase, a pyridino-protein type enzyme which converts glucose to gluconic acid, the pH of the solution being determined colorimetrically; diphosphopyridinonucleatide should be added to this indicator composition. A further enzyme is hexokinase which is used in conjunction with adenosine triphosphate to produce an increase in acidity in the presence of glucose. A preferred indicator composition comprises two enzymes, one of which catalyses the formation of hydrogen peroxide from glucose, and the other, e.g. peroxidase, catalyses the oxidation of another substance by hydrogen peroxide; in place of peroxidase, substances possessing similar activity may be used, examples being hemin, methemoglobin, oxyhemoglobin, hemoglobin, hemochromogen, alkaline hematin, hemin derivatives, iron sulphocyanate, iron tannate, ferrous ferrocyanide, and potassium chromic sulphate on silica gel. Suitable colour-forming substrates are aniline, ortho-toluidine, para-toluidine, ortho-phenylene diamine, N,N1-dimethylpara - phenylenediamine, N,N1 - diethyl - phenylenediamine, benzidine, dianisidine, phenol, thymol, ortho-, meta- and para-cresols, alpha- and beta-naphthols, guaiacol, catechol, orcinol, pyrogallol, p,p - dihydroxydiphenyl, phloroglucinol, benzoic, salicylic, pyrocatechnic, and gallic acids, leucomalachite green, leucophenolphthalein, 2,6-dichlorophenolindophenol, adrenaline, the flavones, tyrosine, dihydroxyphenylalanine, tryptophane, gum guaiac, guaiaconic acid, nadi reagent, water-soluble iodides, and bilirubin. The indicator composition may include a phosphate buffer. The indicator may be in the form of a treated paper, bottled reagent, frangible capsule, a pill or tablet, or a solid polyethylene glycol gel containing the reagent; a heat-generating substance, e.g. lithium chloride, may be included in the pill or tablet. The paper may be treated in strips, each strip containing a reagent of different sensitivity or giving different colours. The colour produced by the test may be measured photo-electrically, colorimetrically, or spectrophotometrically, and may be compared with standards produced by known concentrations of glucose. In examples: (1) an aqueous solution containing ethyl alcohol, o-tolidine, glucose oxidase, horseradish peroxidase, and tartrazine is applied to filter paper and dried; (2)-(8) liquid reagents are prepared from glucose oxidase solution and peroxidase extract, the other ingredients being gum guaiac, with or without phosphate buffer, ortho-phenylene-diamine with or without alpha-naphthol, and potassium or sodium iodide, with or without starch; (9) glucose oxidase solution is mixed with an aqueous solution of bromcresol purple indicator; (10) paper impregnated with a pH dye indicator is sprayed with glucose oxidase solution. Other indicator compositions are referred to, additional ingredients being diaphorase, xanthine oxidase, Zwischenferment, triphosphopyridinonucleotide, old yellow enzyme, and new yellow enzyme.ALSO:A peroxidase extract, for use in a glucose indicator composition (see Group III), is prepared by extracting grated horseradish with water, adding ammonium sulphate to 90 per cent saturation, filtering off the precipitated protein, and dissolving in water. The resulting solution is dialyzed to remove ammonium sulphate and heated to 70 DEG C. for a few minutes to destroy enzymes other than peroxidase.
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US516802A US2981606A (en) | 1955-06-20 | 1955-06-20 | Glucose indicator and method |
GB1971155A GB784548A (en) | 1955-07-07 | 1955-07-07 | Improvements in or relating to glucose indicator |
FR1134095D FR1134095A (en) | 1955-07-07 | 1955-07-11 | Improvements in glucose indicators and a method for determining the amount of glucose |
NL198803A NL198803B (en) | 1955-07-07 | 1955-07-11 | |
CH339755D CH339755A (en) | 1955-07-07 | 1955-07-11 | Glucose indicator |
BE539733A BE539733A (en) | 1955-07-07 | 1955-07-11 | |
AT2458A AT203451B (en) | 1955-07-07 | 1958-01-02 | Bleaching apparatus and parts for acidic chlorite bleaching |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB1971155A GB784548A (en) | 1955-07-07 | 1955-07-07 | Improvements in or relating to glucose indicator |
Publications (1)
Publication Number | Publication Date |
---|---|
GB784548A true GB784548A (en) | 1957-10-09 |
Family
ID=10133934
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB1971155A Expired GB784548A (en) | 1955-06-20 | 1955-07-07 | Improvements in or relating to glucose indicator |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB784548A (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1284124B (en) * | 1959-05-15 | 1968-11-28 | Miles Lab | Diagnostic agent and test indicator for the detection of glucose in body fluids |
FR2314498A1 (en) * | 1975-06-13 | 1977-01-07 | Boehringer Mannheim Gmbh | ENZYMATIC ANALYSIS PROCESS |
CN110923291A (en) * | 2019-12-13 | 2020-03-27 | 山东隆科特酶制剂有限公司 | Method for detecting activity of glucose oxidase for feed |
CN112630440A (en) * | 2020-12-30 | 2021-04-09 | 中国科学院海洋研究所 | Reusable glucose detection box and detection method thereof |
-
1955
- 1955-07-07 GB GB1971155A patent/GB784548A/en not_active Expired
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1284124B (en) * | 1959-05-15 | 1968-11-28 | Miles Lab | Diagnostic agent and test indicator for the detection of glucose in body fluids |
FR2314498A1 (en) * | 1975-06-13 | 1977-01-07 | Boehringer Mannheim Gmbh | ENZYMATIC ANALYSIS PROCESS |
CN110923291A (en) * | 2019-12-13 | 2020-03-27 | 山东隆科特酶制剂有限公司 | Method for detecting activity of glucose oxidase for feed |
CN110923291B (en) * | 2019-12-13 | 2023-06-09 | 山东隆科特酶制剂有限公司 | Method for detecting activity of glucose oxidase for feed |
CN112630440A (en) * | 2020-12-30 | 2021-04-09 | 中国科学院海洋研究所 | Reusable glucose detection box and detection method thereof |
CN112630440B (en) * | 2020-12-30 | 2022-05-10 | 中国科学院海洋研究所 | Reusable glucose detection box and detection method thereof |
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