US20230052697A1 - Assessment of preeclampsia using assays for free and dissociated placental growth factor - Google Patents
Assessment of preeclampsia using assays for free and dissociated placental growth factor Download PDFInfo
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- US20230052697A1 US20230052697A1 US17/710,756 US202217710756A US2023052697A1 US 20230052697 A1 US20230052697 A1 US 20230052697A1 US 202217710756 A US202217710756 A US 202217710756A US 2023052697 A1 US2023052697 A1 US 2023052697A1
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Definitions
- Preeclampsia is a serious multisystem complication of pregnancy.
- the incidence of the disorder is generally considered to be between 2% to 8% of all pregnancies, and the disorder carries significant morbidity and mortality risks for both mothers and infants.
- Preeclampsia is the second largest cause of maternal/fetal deaths and is responsible for approximately twenty billion dollars in healthcare costs annually.
- preeclampsia The cause(s) and pathogenesis of preeclampsia remain uncertain, and the identification (or ruling out) of preeclampsia using the classical clinical symptoms of the disease is non-ideal.
- the presentation of classical clinical symptoms can be highly variable, and the symptoms can be indicative of other distinct disorders, such as chronic hypertension, gestational hypertension, temporary high blood pressure, and gestational diabetes.
- Current laboratories tests e.g., tests for proteinuria
- Methods for more reliably predicting whether a pregnant woman will or will not have preeclampsia may, among other things, (1) lead to more timely diagnosis, (2) improve the accuracy of a diagnosis, and/or (3) prevent the unnecessary treatment of women with some preeclampsia symptoms.
- the present disclosure provides for a method of determining whether a pregnant human female is at risk of suffering from preeclampsia comprising: determining a free Placental Growth Factor to dissociated Placental Growth Factor (PlGF-f:PlGF-d) ratio of a biological sample collected from the pregnant human female, wherein a PlGF-f:PlGF-d ratio less than a first threshold value indicates a heightened risk that the pregnant human female will suffer from preeclampsia, and wherein a PlGF-f:PlGF-d ratio greater than a second threshold value indicates a reduced risk that the pregnant human female will suffer from preeclampsia.
- the method comprises collecting the biological sample.
- the biological sample is a fluid, whole blood, peripheral blood, serum, plasma, urine, or amniotic fluid sample.
- the method comprises collecting the biological sample via venipuncture, fingerstick sampling, or arterial sampling.
- the biological sample is collected from the pregnant woman at gestational week 20 or later.
- the biological sample is collected from the pregnant woman prior to gestational week 30.
- the risk of developing preeclampsia is the risk of developing preeclampsia within the following one, two, three or more weeks.
- the PlGF-f:PlGF-d ratio is determined at least in part by: (a) determining a PlGF-f level by contacting a first PlGF probe to a first portion of the biological sample; and (b) determining a PlGF-d level by contacting the first PlGF probe to a second portion of the biological sample, wherein the first portion of the biological sample is untreated, and wherein a treatment is applied to the second portion of the biological sample, and wherein the treatment dissociates a PlGF complex.
- the PlGF complex comprises PlGF and s-FLT.
- the treatment is applied for at least 5, 10, 15, 20, 25, 30, 40, 50, 60, 90, or 120 minutes.
- the treatment comprises increasing the temperature of the second portion of the biological sample. In some embodiments, the treatment comprises increasing the temperature to at least 30° C., at least 37° C., or at least 45° C. for at least 1 hour or at least 2 hours. In some embodiments, the treatment comprises increasing or decreasing the pH of the second portion of the biological sample. In some embodiments, the treatment comprises contacting the second portion of the biological sample with an agent that prevents PlGF from assembling into a PlGF complex. In some embodiments, the agent is selected from: a surfactant, a detergent, a chaotropic agent, a PlGF-binding protein, and an s-FLT-binding protein.
- the method has a LLOQ for PlGF of 20 pg/ml or less.
- the method comprises (a) determining a level of at least one secondary biomarker selected from total sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274; and (b) determining the pregnant woman as being at risk or not at risk of preeclampsia by application of a trained algorithm to the PlGF-f:PlGF-d ratio in combination with the determined level least one of the secondary biomarker
- the trained algorithm comprises a logistic regression, random forest, nearest centroid, gradient boosting method, linear discriminant analysis, neural network, or support vector machine algorithm.
- the level of the at least one secondary biomarker is determined by an immunoassay.
- the immunoassay is selected from a capture ELISA, an indirect ELISA, a TR-FRET assay, a proximity extension assay, an amplified luminescent proximity (LOCI) assay, a luminescent oxygen channeling immunoassay, or a lateral flow assay.
- LOCI amplified luminescent proximity
- the present disclosure provides for a method of treating a pregnant human female comprising: determining whether the pregnant human female has an elevated risk of preeclampsia or reduced risk of preeclampsia by: obtaining a biological sample from the pregnant human female, determining a PlGF-f:PlGF-d ratio in the biological sample, and optionally determining a level of one or more secondary biomarkers in the biological sample, applying a classifier to the PlGF-f:PlGF-d ratio and, optionally, the level of one or more secondary biomarkers to classify the pregnant human female as having an elevated risk of preeclampsia or a reduced risk of preeclampsia; if the pregnant human female has an elevated risk of preeclampsia, then determining the blood pressure of the pregnant human female at a frequency of at least one time per week, and if the pregnant human female has a reduced risk of preeclampsia, then determining the blood pressure of the pregnant human female at a frequency of less than
- the method comprises, if the pregnant human female is determined to have an elevated risk of preeclampsia, then administering an antihypertensive drug to the pregnant human female.
- the antihypertensive drug is a central alpha agonist, a vasodilator, a calcium-channel blocker, an alpha-blocker, acetylsalicylic acid, or a beta-blocker.
- the antihypertensive drug is methyldopa, labetalol, nifedipine, verapamil, clonidine, hydralazine, diazoxide, prazosin, or oxprenolol.
- the method comprises administering a cervical ripening or labor-inducing agent to the pregnant woman when the pregnant woman is determined to be at risk of suffering from preeclampsia.
- the cervical ripening or labor-inducing agent comprises a prostaglandin, oxytocin, misoprostol, mifepristone, or relaxin.
- the present disclosure provides for a method for avoiding unnecessary treatment of preeclampsia, the method comprising: (a) contacting a biological sample that has been collected from a pregnant human female with a plurality of different antigen-specific probes to determine levels of three or more markers, wherein the three or more markers comprise at least (i) PlGF-f and PlGF-d; and (ii) at least one of sFLT, Endoglin, KIM-1, FGF21, or free sFLT; and (b) proceeding with treatment of the pregnant human in a manner that avoids unnecessary treatment of preeclampsia based at least in part on the amounts or concentrations of the three or more markers determined in (a).
- the method comprises collecting the biological sample.
- the biological sample is a fluid, whole blood, peripheral blood, serum, plasma, urine, or amniotic fluid sample.
- the method comprises collecting the biological sample via venipuncture, fingerstick sampling, or arterial sampling.
- the biological sample is collected from the pregnant woman at gestational week 20 or later.
- the biological sample is collected from the pregnant woman prior to gestational week 30.
- the risk of developing preeclampsia is the risk of developing preeclampsia within the following one, two, three or more weeks.
- the PlGF-f:PlGF-d ratio is determined at least in part by: (a) determining a PlGF-f level by contacting a first PlGF probe to a first portion of the biological sample; and (b) determining a PlGF-d level by contacting the first PlGF probe to a second portion of the biological sample, wherein the first portion of the biological sample is untreated, and wherein a treatment is applied to the second portion of the biological sample, and wherein the treatment dissociates a PlGF complex.
- the PlGF complex comprises PlGF and s-FLT.
- the treatment is applied for at least 5, 10, 15, 20, 25, 30, 40, 50, 60, 90, or 120 minutes.
- the treatment comprises increasing the temperature of the second portion of the biological sample. In some embodiments, the treatment comprises increasing the temperature to at least 30° C., at least 37° C., or at least 45° C. for at least 1 hour or at least 2 hours. In some embodiments, the treatment comprises increasing or decreasing the pH of the second portion of the biological sample. In some embodiments, the treatment comprises contacting the second portion of the biological sample with an agent that prevents PlGF from assembling into a PlGF complex. In some embodiments, the agent is selected from: a surfactant, a detergent, a chaotropic agent, a PlGF-binding protein, and an s-FLT-binding protein.
- the method has a LLOQ for PlGF of 20 pg/ml or less.
- the method comprises (a) determining a level of at least one secondary biomarker selected from total sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274; and (b) determining the pregnant woman as being at risk or not at risk of preeclampsia by application of a trained algorithm to the PlGF-f:PlGF-d ratio in combination with the determined level least one of the secondary biomarker
- the trained algorithm comprises a logistic regression, random forest, nearest centroid, gradient boosting method, linear discriminant analysis, neural network, or support vector machine algorithm.
- the level of the at least one secondary biomarker is determined by an immunoassay.
- the immunoassay is selected from a capture ELISA, an indirect ELISA, a TR-FRET assay, a proximity extension assay, an amplified luminescent proximity (LOCI) assay, a luminescent oxygen channeling immunoassay, or a lateral flow assay.
- LOCI amplified luminescent proximity
- the present disclosure provides for a system for ruling out preeclampsia in a pregnant female subject for a specified period of time, the system comprising: a processor; an input module for inputting levels of at least three markers in a biological sample, wherein the at least three markers comprise at least (i) PlGF-f and PlGF-d; and (ii) at least one of sFLT, Endoglin, KIM-1, FGF21, or free sFLT; a computer readable medium containing instructions that, when executed by the processor, perform a first algorithm on the input levels of the at least three markers; and an output module providing one or more indicia based on the input levels of the at least three markers, wherein the one or more indicia are indicative of the subject not having preeclampsia for at least a specified period of time.
- the biological sample is a fluid, whole blood, peripheral blood, serum, plasma, urine, or amniotic fluid sample. In some embodiments, the biological sample is collected from the pregnant woman at gestational week 20 or later. In some embodiments, the biological sample is collected from the pregnant woman prior to gestational week 30. In some embodiments, the risk of developing preeclampsia is the risk of developing preeclampsia within the following one, two, three or more weeks.
- the PlGF-f:PlGF-d ratio is determined at least in part by: (a) determining a PlGF-f level by contacting a first PlGF probe to a first portion of the biological sample; and (b) determining a PlGF-d level by contacting the first PlGF probe to a second portion of the biological sample, wherein the first portion of the biological sample is untreated, and wherein a treatment is applied to the second portion of the biological sample, and wherein the treatment dissociates a PlGF complex.
- the PlGF complex comprises PlGF and s-FLT.
- the treatment is applied for at least 5, 10, 15, 20, 25, 30, 40, 50, 60, 90, or 120 minutes.
- the treatment comprises increasing the temperature of the second portion of the biological sample. In some embodiments, the treatment comprises increasing the temperature to at least 30° C., at least 37° C., or at least 45° C. for at least 1 hour or at least 2 hours. In some embodiments, the treatment comprises increasing or decreasing the pH of the second portion of the biological sample. In some embodiments, the treatment comprises contacting the second portion of the biological sample with an agent that prevents PlGF from assembling into a PlGF complex. In some embodiments, the agent is selected from: a surfactant, a detergent, a chaotropic agent, a PlGF-binding protein, and an s-FLT-binding protein.
- the method has a LLOQ for PlGF of 20 pg/ml or less.
- the method comprises (a) determining a level of at least one secondary biomarker selected from total sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274; and (b) determining the pregnant woman as being at risk or not at risk of preeclampsia by application of a trained algorithm to the PlGF-f:PlGF-d ratio in combination with the determined level least one of the secondary biomarker
- the trained algorithm comprises a logistic regression, random forest, nearest centroid, gradient boosting method, linear discriminant analysis, neural network, or support vector machine algorithm.
- the level of the at least one secondary biomarker is determined by an immunoassay.
- the immunoassay is selected from a capture ELISA, an indirect ELISA, a TR-FRET assay, a proximity extension assay, an amplified luminescent proximity (LOCI) assay, a luminescent oxygen channeling immunoassay, or a lateral flow assay.
- LOCI amplified luminescent proximity
- the present disclosure provides for a method for determining a PlGF-f:PlGF-d ratio in a biological sample comprising: (a) isolating a first aliquot of the biological sample for the detection of PlGF-f and a second aliquot of the biological sample for the detection of PlGF-d; (b) determining an amount of PlGF-f in the first aliquot; (b) applying a treatment to the second aliquot to dissociate PlGF complexes; and (c) determining an amount of PlGF-d in the second aliquot.
- the biological sample has not been stored at a temperature above about 20° C.
- the amount of PlGF-f and the amount of PlGF-d are determined using the same reagents.
- the PlGF complex comprises PlGF and s-FLT.
- the treatment is applied for at least 5, 10, 15, 20, 25, 30, 40, 50, 60, 90, or 120 minutes.
- the treatment comprises increasing the temperature of the second portion of the biological sample.
- the treatment comprises increasing the temperature to at least 30° C. for at least 1 hour.
- the treatment comprises increasing or decreasing the pH of the second portion of the biological sample.
- the treatment comprises contacting the second portion of the biological sample with an agent that prevents PlGF from assembling into a PlGF complex.
- the agent is selected from: a surfactant, a detergent, a chaotropic agent, a PlGF-binding protein, and an s-FLT-binding protein.
- the method has a LLOQ for PlGF of 20 pg/ml or less.
- the present disclosure provides for a method for determining a PlGF-f:PlGF-d ratio in a biological sample comprising PlGF, the method comprising, (a) determining an amount of PlGF-f in a first aliquot of the biological sample by: (i) contacting the first aliquot with a capture reagent for a first time at a first temperature, wherein the PIGF-f binds to the capture reagent to form a PIGF-f-capture reagent complex; (ii) contacting the PIGF-f-capture reagent complex with a detector reagent for a second time at a second temperature, wherein the PIGF-f-capture reagent complex binds to the second reagent to form a first ternary complex comprising PIGF-f, the capture reagent, and the detector reagent; and (iii) detecting the first ternary complex; and (b) determining an amount of PlGF-d in a second aliquot of the
- the first temperature is less than 30° C., less than 25° C., between 15° C. and 30° C., between 15° C. and 25° C., between 20° C. and 25° C., or about 22° C.
- the second temperature is less than 30 C, less than 25 C, between 15 C and 30 C, between 15° C. and 25° C., between 20° C. and 25° C., or about 22° C.
- the third temperature is at least 30° C., at least 35° C., between 30° C. and 45° C., about 30° C., about 37° C.
- the fourth temperature is at least 30° C., at least 35° C., between 30° C. and 45° C., about 30° C., about 37° C. or about 45° C.;
- the first time is less than 1 hour, less than 45 minutes, between 10 minutes and 30 minutes, about 30 minutes, about 20 minutes, or about 15 minutes;
- the second time is less than 1 hour, less than 45 minutes, between 10 minutes and 30 minutes, about 30 minutes, about 20 minutes, or about 15 minutes;
- the third time is at least 1 hour, between 1 hour and 3 hours, between 90 minutes and 150 minutes, or about 120 minutes; and
- the fourth time is at least 1 hour, between 1 hour and 3 hours, between 90 minutes and 150 minutes, or about 120 minutes.
- the first time is about 30 minutes
- the second time is about 30 minutes
- the third time is about 2 hours
- the fourth time is about 2 hours
- the first temperature is about 22° C.
- the second temperature is about 22° C.
- the third temperature is about 37° C.
- the fourth temperature is about 37° C.
- step b is conducted in the presence of a blocking antibody that prevents s-FLT from rebinding to PlGF.
- the biological sample is blood, serum, plasma, amniotic fluid, or urine.
- the biological sample is serum or plasma.
- the biological sample is collected from a pregnant human female. In some embodiments, the pregnant human female has been pregnant for at least 20 weeks.
- the pregnant human female has been pregnant for no more than 30 weeks.
- the biological sample is stored at a storage temperature and the storage temperature never exceeds 30° C. In some embodiments, the storage temperature is less than 0° C. In some embodiments, the storage temperature is about ⁇ 80° C.
- the biological sample is diluted prior to step a.
- the capture reagent comprises an antibody or an antigen-binding fragment thereof.
- the detector reagent comprises an antibody or an antigen-binding fragment thereof.
- the capture reagent is attached to a solid support. In some embodiments, the detector reagent is attached to a solid support.
- the detector reagent comprises a detectable label.
- the label is a fluorophore, an enzyme, or an electrochemiluminescent tag.
- (a) further comprises contacting the first aliquot with a crosslinking agent that prevents the dissociation of a PlGF-sFLT complex.
- the present disclosure provides for a method for determining a PlGF-f:PlGF-d ratio in a biological sample, wherein PlGF-f is determined using a reagent that can bind to PlGF-f but cannot bind to PlGF-d.
- the reagent is an antibody or antigen-binding fragment thereof.
- the reagent binds to residues on the PlGF-1 molecule that are involved in recognition of s-Flt-1.
- the present disclosure provides for a method for determining a PlGF-f:PlGF-d ratio in a biological sample, wherein PlGF-d is determined using a reagent that can bind to PlGF-d but cannot bind to PlGF-f.
- the reagent is an antibody or antigen-binding fragment thereof.
- the reagent binds to the interface between PlGF and s-Flt-1.
- the present disclosure provides for a method for determining a PlGF-f:PlGF-d ratio in a biological sample comprising separating the biological sample into a free PlGF component and a PlGF complex component, determining the amount of PlGF in the free PlGF component, and determining the amount of PlGF in the PlGF complex component.
- the separating is performed by anion exchange chromatography, cation exchange chromatography, gel filtration chromatography or filtration.
- the biological sample is contacted with a chemical crosslinking agent prior to the separating.
- the present disclosure provides for a method of determining whether a pregnant human female is at risk of suffering from preeclampsia comprising: (a) determining a free Placental Growth Factor to dissociated Placental Growth Factor (PlGF-f:PlGF-d) ratio of a biological sample collected from the pregnant human female, wherein a PlGF-f:PlGF-d ratio less than a first threshold value indicates a heightened risk that the pregnant human female will suffer from preeclampsia, and wherein a PlGF-f:PlGF-d ratio greater than a second threshold value indicates a reduced risk that the pregnant human female will suffer from preeclampsia.
- PlGF-f:PlGF-d free Placental Growth Factor to dissociated Placental Growth Factor
- the method comprises collecting the biological sample.
- the biological sample is a fluid, whole blood, peripheral blood, serum, plasma, urine, or amniotic fluid sample.
- the method further comprises collecting the biological sample via venipuncture, fingerstick sampling, or arterial sampling.
- the biological sample is collected from the pregnant woman at gestational week 20 or later.
- the biological sample is collected from the pregnant woman at gestational week 28 or later.
- the biological sample is collected from the pregnant woman at gestational week 35 or later.
- the biological sample is collected from the pregnant woman prior to gestational week 30.
- the biological sample is collected from the pregnant woman prior to gestational week 37. In some embodiments, the biological sample is collected from the pregnant woman at gestational week 28 to 36 6/7. In some embodiments, the biological sample is collected from the pregnant woman at gestational week 35 to 36 6/7. In some embodiments, the risk of developing preeclampsia is the risk of developing preeclampsia within the following one, two, three or more weeks. In some embodiments, the risk of developing preeclampsia is the risk of developing preeclampsia within the following two weeks.
- the PlGF-f:PlGF-d ratio is determined at least in part by: (a) determining a PlGF-f level by contacting a first PlGF probe to a first portion of the biological sample; and (b) determining a PlGF-d level by contacting the first PlGF probe to a second portion of the biological sample, wherein the first portion of the biological sample is untreated, and wherein a treatment is applied to the second portion of the biological sample wherein the treatment dissociates a PlGF complex.
- the PlGF complex comprises PlGF and s-FLT.
- the treatment is applied for at least 5, 10, 15, 20, 25, 30, 40, 50, 60, 90, or 120 minutes.
- the treatment comprises increasing the temperature of the second portion of the biological sample. In some embodiments, the treatment comprises increasing the temperature to at least 30° C., at least 37° C., or at least 45° C. for at least 1 hour or at least 2 hours. In some embodiments, the treatment comprises increasing or decreasing the pH of the second portion of the biological sample. In some embodiments, the treatment comprises contacting the second portion of the biological sample with an agent that prevents PlGF from assembling into a PlGF complex. In some embodiments, the agent is selected from: a surfactant, a detergent, a chaotropic agent, a PlGF-binding protein, and an s-FLT-binding protein.
- the first unmodified portion of the biological sample has not been stored at a temperature above about 20° C.
- the method has a LLOQ for PlGF of 20 pg/ml or less.
- the method further comprises (a) determining a level of at least one secondary biomarker selected from total sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274; and (b) determining the pregnant woman as being at risk or not at risk of preeclampsia by application of a trained algorithm to the PlGF-f:PlGF-d ratio in combination with the determined level of the at least one secondary biomarker.
- the trained algorithm comprises a logistic regression, random forest, nearest centroid, gradient boosting method, linear discriminant analysis, neural network, or support vector machine algorithm.
- the level of the at least one secondary biomarker is determined by an immunoassay.
- the immunoassay is selected from a capture ELISA, an indirect ELISA, a TR-FRET assay, a proximity extension assay, an amplified luminescent proximity (LOCI) assay, a luminescent oxygen channeling immunoassay, or a lateral flow assay.
- the method further comprises proceeding with treatment of the pregnant human in a manner that avoids unnecessary treatment of preeclampsia comprises administering an anti-hypertensive drug or repeating (a) at a particular frequency.
- the method further comprises administering an anti-hypertensive drug, wherein the antihypertensive drug is a central alpha agonist, a vasodilator, a calcium-channel blocker, an alpha-blocker, acetylsalicylic acid, or a beta-blocker.
- the present disclosure provides for a method of determining whether a pregnant human female is at risk of suffering from preeclampsia comprising: (a) determining a level of at least PlGF-f.
- a PlGF-f level less than a first threshold value indicates a heightened risk that the pregnant human female will suffer from preeclampsia.
- a PlGF-f level greater than a second threshold value indicates a reduced risk that the pregnant human female will suffer from preeclampsia.
- the first threshold value and the second threshold value are the same value.
- the method further comprises determining a level of PlGF-d.
- the method further comprises determining a ratio of PlGF-f to PlGF-d. In some embodiments, a ratio of PlGF-f to PlGF-d less than a first threshold value indicates a reduced risk the pregnant human female will suffer from preeclampsia. In some embodiments, a ratio of PlGF-f to PlGF-d greater than a second threshold value indicates an increased risk the pregnant human female will suffer from preeclampsia. In some embodiments, the method comprises collecting the biological sample. In some embodiments, the biological sample is a fluid, whole blood, peripheral blood, serum, plasma, urine, or amniotic fluid sample. In some embodiments, the method further comprises collecting the biological sample via venipuncture, fingerstick sampling, or arterial sampling.
- the biological sample is collected from the pregnant woman at gestational week 20 or later. In some embodiments, the biological sample is collected from the pregnant woman at gestational week 28 or later. In some embodiments, the biological sample is collected from the pregnant woman at gestational week 35 or later. In some embodiments, the biological sample is collected from the pregnant woman prior to gestational week 30. In some embodiments, the biological sample is collected from the pregnant woman prior to gestational week 37. In some embodiments, the biological sample is collected from the pregnant woman at gestational week 28 to 36 6/7. In some embodiments, the biological sample is collected from the pregnant woman at gestational week 35 to 36 6/7.
- the risk of developing preeclampsia is the risk of developing preeclampsia within the following one, two, three or more weeks. In some embodiments, the risk of developing preeclampsia is the risk of developing preeclampsia within the following two weeks.
- the PlGF-f:PlGF-d ratio is determined at least in part by: (a) determining a PlGF-f level by contacting a first PlGF probe to a first portion of the biological sample; and (b) determining a PlGF-d level by contacting the first PlGF probe to a second portion of the biological sample, wherein the first portion of the biological sample is untreated, and wherein a treatment is applied to the second portion of the biological sample wherein the treatment dissociates a PlGF complex.
- the PlGF complex comprises PlGF and s-FLT.
- the treatment is applied for at least 5, 10, 15, 20, 25, 30, 40, 50, 60, 90, or 120 minutes.
- the treatment comprises increasing the temperature of the second portion of the biological sample. In some embodiments, the treatment comprises increasing the temperature to at least 30° C., at least 37° C., or at least 45° C. for at least 1 hour or at least 2 hours. In some embodiments, the treatment comprises increasing or decreasing the pH of the second portion of the biological sample. In some embodiments, the treatment comprises contacting the second portion of the biological sample with an agent that prevents PlGF from assembling into a PlGF complex. In some embodiments, the agent is selected from: a surfactant, a detergent, a chaotropic agent, a PlGF-binding protein, and an s-FLT-binding protein.
- the first unmodified portion of the biological sample has not been stored at a temperature above about 20° C.
- the method has a LLOQ for PlGF of 20 pg/ml or less.
- the method further comprises (a) determining a level of at least one secondary biomarker selected from total sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274; and (b) determining the pregnant woman as being at risk or not at risk of preeclampsia by application of a trained algorithm to the PlGF-f:PlGF-d ratio in combination with the determined level of the at least one secondary biomarker.
- the trained algorithm comprises a logistic regression, random forest, nearest centroid, gradient boosting method, linear discriminant analysis, neural network, or support vector machine algorithm.
- the level of the at least one secondary biomarker is determined by an immunoassay.
- the immunoassay is selected from a capture ELISA, an indirect ELISA, a TR-FRET assay, a proximity extension assay, an amplified luminescent proximity (LOCI) assay, a luminescent oxygen channeling immunoassay, or a lateral flow assay.
- the method further comprises proceeding with treatment of the pregnant human in a manner that avoids unnecessary treatment of preeclampsia comprises administering an anti-hypertensive drug or repeating (a) at a particular frequency.
- the method further comprises administering an anti-hypertensive drug, wherein the antihypertensive drug is a central alpha agonist, a vasodilator, a calcium-channel blocker, an alpha-blocker, acetylsalicylic acid, or a beta-blocker.
- the present disclosure provides for a method of treating a pregnant human female comprising the steps of: determining whether the pregnant human female has an elevated risk of preeclampsia or reduced risk of preeclampsia by: obtaining a biological sample from the pregnant human female, determining a PlGF-f:PlGF-d ratio in the biological sample, and optionally determining a level of one or more secondary biomarkers in the biological sample; applying a classifier to the PlGF-f:PlGF-d ratio and, optionally, the level of one or more secondary biomarkers to classify the pregnant human female as having an elevated risk of preeclampsia or a reduced risk of preeclampsia; if the pregnant human female has an elevated risk of preeclampsia, then determining the blood pressure of the pregnant human female at a frequency of at least one time per week, and if the pregnant human female has a reduced risk of preeclampsia, then determining the blood pressure of the pregnant human female at a frequency
- the pregnant human female is determined to not have an elevated risk of preeclampsia, then administering an antihypertensive drug to the pregnant human female.
- the antihypertensive drug is a central alpha agonist, a vasodilator, a calcium-channel blocker, an alpha-blocker, acetylsalicylic acid, or a beta-blocker.
- the antihypertensive drug is methyldopa, labetalol, nifedipine, verapamil, clonidine, hydralazine, diazoxide, prazosin, or oxprenolol.
- the method further comprises administering a cervical ripening or labor-inducing agent to the pregnant woman when the pregnant woman is determined to be at risk of suffering from preeclampsia.
- the cervical ripening or labor-inducing agent comprises a prostaglandin, oxytocin, misoprostol, mifepristone, or relaxin.
- the present disclosure provides for a method of treating a pregnant human female comprising the steps of: determining whether the pregnant human female has an elevated risk of preeclampsia or reduced risk of preeclampsia by: obtaining a biological sample from the pregnant human female, determining a level of at least PlGF-f in the biological sample, and optionally determining a level of one or more secondary biomarkers in the biological sample.
- the method further comprises applying a classifier to the PlGF-f level and, optionally, the level of one or more secondary biomarkers to classify the pregnant human female as having an elevated risk of preeclampsia or a reduced risk of preeclampsia.
- the method further comprises determining a level of PlGF-d and applying a classifier to the PlGF-d level. In some embodiments, if the pregnant human female has an elevated risk of preeclampsia, then the method comprises determining the blood pressure of the pregnant human female at a frequency of at least one time per week. In some embodiments, if the pregnant human female has a reduced risk of preeclampsia, then the method further comprises determining the blood pressure of the pregnant human female at a frequency of less than one time per week. In some embodiments, when the pregnant human female is determined to not have an elevated risk of preeclampsia, then the method further comprises administering an antihypertensive drug to the pregnant human female.
- the antihypertensive drug is a central alpha agonist, a vasodilator, a calcium-channel blocker, an alpha-blocker, acetylsalicylic acid, or a beta-blocker.
- the antihypertensive drug is methyldopa, labetalol, nifedipine, verapamil, clonidine, hydralazine, diazoxide, prazosin, or oxprenolol.
- the method further comprises administering a cervical ripening or labor-inducing agent to the pregnant woman when the pregnant woman is determined to be at risk of suffering from preeclampsia.
- the cervical ripening or labor-inducing agent comprises a prostaglandin, oxytocin, misoprostol, mifepristone, or relaxin.
- the present disclosure provides for a method for avoiding unnecessary treatment of preeclampsia, the method comprising: (a) contacting a biological sample that has been collected from a pregnant human female with a plurality of different antigen-specific probes to determine levels of two or more markers, wherein the two or more markers comprise at least PlGF-f and PlGF-d; and (b) proceeding with treatment of the pregnant human in a manner that avoids unnecessary treatment of preeclampsia based at least in part on the amounts or concentrations of the markers determined in (a).
- the two or more markers comprise three or more markers further comprising at least one of sFLT, Endoglin, KIM-1, FGF21, or free sFLT.
- the present disclosure provides for a system for ruling out preeclampsia in a pregnant female subject for a specified period of time, the system comprising: a processor; an input module for inputting levels of at least two markers in a biological sample, wherein the at least two markers comprise at least PlGF-f and PlGF-d; and a computer readable medium containing instructions that, when executed by the processor, performs a first algorithm on the input levels of the at least two markers.
- the at least two markers comprise at least three markers that further comprise at least one of sFLT, Endoglin, KIM-1, FGF21, or free sFLT.
- the system further comprises an output module providing one or more indicia based on the input levels of the at least three markers, wherein the one or more indicia are indicative of the subject not having preeclampsia for at least a specified period of time.
- the present disclosure provides for a method for determining levels of free and dissociated PlGF in a biological sample from a subject, the method comprising: (a) isolating a first aliquot of the biological sample for the detection of PlGF-f and a second aliquot of the biological sample for the detection of PlGF-d; (b) determining an amount of PlGF-f in the first aliquot; (c) applying a treatment to the second aliquot to dissociate PlGF complexes; and (d) determining an amount of PlGF-d in the second aliquot.
- the method further comprises determining a ratio of the amounts of PlGF-f and PlGF-d.
- the biological sample has not been stored at a temperature above about 20° C.
- the amount of PlGF-f and the amount of PlGF-d are determined using same reagents.
- the PlGF complex comprises PlGF and s-FLT.
- the treatment is applied for at least 5, 10, 15, 20, 25, 30, 40, 50, 60, 90, or 120 minutes.
- the treatment comprises increasing the temperature of the second portion of the biological sample.
- the treatment comprises increasing the temperature to at least 30° C. for at least 1 hour.
- the treatment comprises increasing or decreasing the pH of the second portion of the biological sample.
- the treatment comprises contacting the second portion of the biological sample with an agent that prevents PlGF from assembling into a PlGF complex or disables PlGF from a PlGF:sFLT1 complex.
- the agent is selected from: a surfactant, a detergent, a chaotropic agent, a PlGF-binding protein, and an s-FLT-binding protein.
- the method has a LLOQ for PlGF of 20 pg/ml or less.
- the present disclosure provides for a method for determining free and dissociated PlGF amounts in a biological sample comprising PlGF, the method comprising: (a) determining an amount of PlGF-f in a first aliquot of the biological sample by: (i) contacting the first aliquot with a capture reagent for a first time at a first temperature, wherein the PIGF-f binds to the capture reagent to form a PIGF-f-capture reagent complex; (ii) contacting the PIGF-f-capture reagent complex with a detector reagent for a second time at a second temperature, wherein the PIGF-f-capture reagent complex binds to the second reagent to form a first ternary complex comprising PIGF-f, the capture reagent, and the detector reagent; and (iii) detecting the first ternary complex; and (b) determining an amount of PlGF-d in a second aliquot of the biological sample
- the first temperature is less than 30° C., less than 25° C., between 15° C. and 30° C., between 15° C. and 25° C., between 20° C. and 25° C., or about 22° C.
- the second temperature is less than 30 C, less than 25 C, between 15 C and 30 C, between 15° C. and 25° C., between 20° C. and 25° C., or about 22° C.
- the third temperature is at least 30° C., at least 35° C., between 30° C. and 45° C., about 30° C., about 37° C.
- the fourth temperature is at least 30° C., at least 35° C., between 30° C. and 45° C., about 30° C., about 37° C. or about 45° C.;
- the first time is less than 1 hour, less than 45 minutes, between 10 minutes and 30 minutes, about 30 minutes, about 20 minutes, or about 15 minutes;
- the second time is less than 1 hour, less than 45 minutes, between 10 minutes and 30 minutes, about 30 minutes, about 20 minutes, or about 15 minutes;
- the third time is at least 1 hour, between 1 hour and 3 hours, between 90 minutes and 150 minutes, or about 120 minutes; or (h) the fourth time is at least 1 hour, between 1 hour and 3 hours, between 90 minutes and 150 minutes, or about 120 minutes.
- the first time is about 30 minutes
- the second time is about 30 minutes
- the third time is about 2 hours
- the fourth time is about 2 hours
- the first temperature is about 22° C.
- the second temperature is about 22° C.
- the third temperature is about 37° C.
- the fourth temperature is about 37° C.
- step b is conducted in the presence of a blocking antibody that prevents s-FLT from rebinding to PlGF.
- the biological sample is blood, serum, plasma, amniotic fluid, or urine.
- the biological sample is serum or plasma.
- the biological sample is collected from a pregnant human female. In some embodiments, the pregnant human female has been pregnant for at least 20 weeks.
- the pregnant human female has been pregnant for no more than 30 weeks.
- the biological sample is stored at a storage temperature and the storage temperature never exceeds 30° C. In some embodiments, the storage temperature is less than 0° C. In some embodiments, the storage temperature is about ⁇ 80° C.
- the biological sample is diluted prior to step a.
- the capture reagent comprises an antibody or an antigen-binding fragment thereof.
- the detector reagent comprises an antibody or an antigen-binding fragment thereof.
- the capture reagent is attached to a solid support. In some embodiments, the detector reagent is attached to a solid support.
- the detector reagent comprises a detectable label.
- the label is a fluorophore, an enzyme, or an electrochemiluminescent tag.
- (a) further comprises contacting the first aliquot with a crosslinking agent that prevents the dissociation of a PlGF-sFLT complex.
- the present disclosure provides for a method for determining a PlGF-f:PlGF-d ratio in a biological sample, wherein PlGF-f is determined using a reagent that can bind to PlGF-f but cannot bind to PlGF-d.
- the reagent is an antibody or antigen-binding fragment thereof.
- the reagent binds to residues on the PlGF-1 molecule that are involved in recognition of sFlt-1.
- the present disclosure provides for a method for determining a PlGF-f:PlGF-d ratio in a biological sample, wherein PlGF-d is determined using a reagent that can bind to PlGF-d but cannot bind to PlGF-f.
- the reagent is an antibody or antigen-binding fragment thereof.
- the reagent binds to an interface between PlGF and sFlt-1.
- the present disclosure provides for a method for determining free and dissociated PlGF amounts in a biological sample comprising separating the biological sample into a free PlGF component and a PlGF complex component, determining the amount of PlGF in the free PlGF component, and determining the amount of PlGF in the PlGF complex component.
- the separating is performed by anion exchange chromatography, cation exchange chromatography, gel filtration chromatography or filtration.
- the biological sample is contacted with a chemical crosslinking agent prior to the separating.
- the present disclosure provides for a kit for assessing a risk of preeclampsia or ruling out preeclampsia in a pregnant female subject, the kit comprising: (a) an agent or apparatus for dissociating placental growth factor (PLGF) from a PLGF complex with another protein; and (b) at least one probe for specific binding to placental growth factor (PlGF).
- the kit is designed to measure the levels of no more than 20, no more than 15, no more than 10, nor more than 8, no more than 7, no more than 6, nor more than 5, or no more than 4 proteins.
- the kit is further designed to measure the levels of at least one of sFLT, Endoglin, KIM-1, FGF21, or free sFLT
- the kit further comprises at least one probe for specific binding to at least one of sFLT, Endoglin, KIM-1, FGF21, or free sFLT
- the kit further comprises instructions for carrying out an immunoassay.
- the kit is an enzyme-linked immunosorbent assay kit.
- one or more of the probes are attached to a substrate.
- the kit is for a lateral flow immunoassay.
- the kit further comprises a collection tube for collecting a biological sample from the pregnant female subject.
- the collection tube is a collection tube suitable for collection of serum or plasma.
- the at least one probe for specific binding to PlGF or the least one probe for specific binding to at least one of sFLT, Endoglin, KIM-1, FGF21, or free sFLT comprises an antibody or antigen-binding fragment or derivative thereof.
- the kit further comprising a microwell or multiwell plate.
- the microwell or multiwell plate comprises at least one well with the at least one probe for specific binding to PlGF or the least one probe for specific binding to at least one of sFLT, Endoglin, KIM-1, FGF21, or free sFLT conjugated to a surface therein.
- PlGF(f) or PlGF-f refers to free Placental Growth Factor (PlGF)
- PlGF(d) or PlGF-d refers to disassociated PlGF as defined herein.
- FIGS. 1 A and 1 B depict schemes showing the species of PlGF predicted to be present in pregnant patient serum samples (A), as well as the predicted behavior of these species in sandwich ELISA assays (B).
- PlGF species can include free PlGF (e.g., in dimerized form), PLGF(2:1) (e.g., PlGF dimer and single sFLT molecule), and PlGF(2:2) (e.g., PlGF dimer and two sFLT molecules).
- FIGS. 2 A and 2 B illustrate the change in measured PlGF concentration of PE and Non-PE samples incubated at various capture/detection temperatures (A), as well as the predicted behavior of different PlGF species during sample capture and detection incubation (B).
- FIGS. 3 A and 3 B illustrate the effects of various capture temperatures and sFLT concentrations on the detection of PlGF using 3rd trimester pregnancy samples.
- FIGS. 4 A, 4 B, 4 C, and 4 D illustrate the effects of various capture times and sFLT concentrations on the detection of PlGF using 3rd trimester pregnancy samples.
- FIGS. 5 A and 5 B illustrate a pilot study of where PlGF was measured at various temperatures—22° C. for PlGF(f), 30° C. for PlGF(II), and 37° C. for PlGF(d)—and in the presence of various amounts of sFLT in serum from patients with and without preeclampsia.
- FIG. 6 shows plots of PE and non-PE patients from the studies segregated using PlGF(f) conc or % free PlGF ((f)/(d) ⁇ 100) demonstrating that both metrics separate PE samples from non-PE samples and % free PlGF shows improved separation.
- blue dot indicates Non-PE
- orange dot indicates PE
- grey dot indicates PE from 104 cohort
- * indicates below LLOQ.
- FIGS. 7 A, 7 B, and 7 C show PlGF measurements of a larger set of PE and non-PE serum samples using different protocols for measuring species of PlGF—22 C for PlGF(f), 30 C for PlGF(II), and 37 C for PlGF(d)—and in the presence of various amounts of sFLT, demonstrating that % free PlGF can improve separation of PE from non-PE samples.
- FIG. 8 illustrates determination of performance metrics for PlGF(f), PlGF(d), and PlGF (% f) alongside the old PlGF protocol (“PlGF(II)” as markers to discriminate PE from non-PE patients.
- FIG. 9 illustrates a calibration curve using recombinant PlGF using the optimized conditions described in Example 2 which was used to estimate LLOQ for PlGF(f) (10 pg/ml) and PlGF(d) (20 pg/ml) species.
- FIG. 10 illustrates analysis of PlGF(d), PlGF(f), and PlGF (% free) versus gestational age (GA), showing that free PlGF offers a robust segregation into earlier gestational age ( ⁇ 28 weeks), indicating that free PlGF (or modifications thereof) could further enable stratification of the preeclampsia cohort at earlier gestational ages.
- the separation of non-PE and PE lines for dissociated PlGF, free PlGF, and % free PlGF indicates that the ratio of free to dissociated PlGF can allow for normalization within a patient while maintaining separation between non-PE and PE samples. Further, it appears low dissociated PlGF may identify a subset of patients with a different risk profile.
- FIG. 11 shows an exemplary computer system that can be used alongside the methods, compositions, and systems described herein.
- FIG. 12 shows an AUC analysis for a univariate model based on % free PlGF as described in Example 5.
- FIG. 13 depicts cutoff parameters for the univariate model based on % free PlGF as described in Example 5.
- FIG. 14 depicts inclusion/exclusion criteria for the PRO-129 study used to validate the models and clinical profile/model combinations described herein.
- FIGS. 15 A and 15 B show the different definitions of clinical categories used in cross-sectional and forward-looking “rule out” analyses performed in Examples 5 and 7.
- preeclampsia Detection of preeclampsia using the classic clinical symptoms of the disease is error-prone, risking significant adverse outcome for patients and an added burden to the healthcare system through misdiagnosis.
- Measurement of proteinuria is prone to inaccuracies (e.g., false negatives and false positives), preeclampsia complications may occur before proteinuria becomes significant, the clinical presentation of preeclampsia can be highly variable in progression rate, onset time, and severity, and the symptoms (hypertension, proteinuria, or both) can be indicative of other distinct disorders that could utilize a less aggressive treatment course (chronic hypertension, gestational hypertension, temporary high blood pressure, and gestational diabetes).
- hypertension refers to abnormally high blood pressure. Hypertension can be identified in any suitable manner, such as by reference to a sitting systolic blood pressure (sSBP) of greater than 140 mmHg or a sitting diastolic blood pressure (sDBP) of greater than 90 mmHg. Hypertension can be further classified into class 1 or class 2 hypertension, with class 1 exhibiting sSBP of 140-149 mmHg or 90-99 mmHg sDBP, and class 2 exhibiting greater than 160 mmHg sSBP or 100 mmHg sDBP. (See, e.g., The Seventh Report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure.
- Hypertension can also be determined according to the 2017 AHA guidelines (see Whelton et al. 2017 ACC/AHA/AAPA/ABC/ACPM/AGS/APhA/ASH/ASPC/NMA/PCNA Guideline for the Prevention, Detection, Evaluation, and Management of High Blood Pressure in Adults, Journal of the American College of Cardiology (2017), doi: 10.1016/j.jacc.2017.11.006).
- Such guidelines identify “normal” blood pressure as less than 120/80 mmHg, “elevated” as systolic between 120-129 mmHg and diastolic less than 80 mmHg, “stage 1” as systolic between 120-139 mmHg or diastolic between 80-89 mmHg, “stage 2” as systolic at least 140 mmHg or diastolic at least 90 mmHg, and “hypertensive crisis” as systolic over 180 and/or diastolic over 120.
- proteinuria is defined as the presence of abnormal protein in the urine.
- a number of indicator dyes and reagents can used to measure proteinuria semi-quantitatively (e.g., bromophenol blue).
- concentrations of protein in urine can be determined by a semi quantitative “dipstick” analysis and graded as negative, trace (10-20 mg/dL), 1+( ⁇ 30 mg/dL), 2+( ⁇ 100 mg/dL), 3+( ⁇ 300 mg/dL), or 4+(1,000 mg/dL), with 2+ commonly being used as the threshold for problematic proteinuria.
- concentrations of protein in urine can also be measured per 24-hour urine collection, in which greater than or equal to 300 mg protein indicates proteinuria.
- concentrations of protein in urine can be measured in a spot urine sample, in which 30 mg of protein per deciliter or greater indicates proteinuria.
- proteinuria can also be expressed as the protein/creatinine ratio (Pr/Cr) in urine, in which a Pr/Cr ratio of >0.3 is indicative of problematic proteinuria.
- antibody or fragments thereof is used in the broadest sense and specifically encompasses intact monoclonal antibodies, polyclonal antibodies, multispecific antibodies (e.g. bispecific antibodies formed from at least one binding domain of two intact antibodies), and antibody fragments.
- Antibody fragments comprise a portion of an intact antibody that retains antigen-binding activity; examples include Fab, Fab′, F(ab) 2 , F(abc) 2 , and Fv fragments as well as diabodies, linear antibodies, scFvs, and multispecific antibodies formed from antibody fragments.
- preeclampsia refers to a pregnancy-specific disorder involving multiple organ systems thought to originate from abnormal placentation, dysfunctional trophoblast development, defective placental angiogenesis, and a heightened systemic inflammatory response in the mother.
- Preeclampsia when untreated, can progress to ecclampsia, HELLP syndrome, hemorrhagic or ischemic stroke, liver damage, acute kidney injury, and acute respiratory distress syndrome (ARDS).
- preeclampsia frequently presents with symptoms such as hypertension and proteinuria
- symptoms such as hypertension and proteinuria
- symptoms reflective of vascular tension disorders and kidney dysfunction on their own therefore have less than ideal predictive/diagnostic value for preeclampsia.
- Further information on the pathophysiology of preeclampsia can be found, e.g., in Phipps et al. Clin J Am Soc Nephrol 11(2016):1102-1113.
- a traditional diagnosis of preeclampsia is made when hypertension and proteinuria as defined above are detected at the same time.
- a traditional diagnosis of preeclampsia is made upon simultaneous detection of hypertension (blood pressure greater than or equal to 140 mmHg systolic or greater than or equal to 90 mmHg diastolic on two occasions at least 4 hours apart after 20 weeks gestation in a woman with a previously normal blood pressure, or blood pressure with greater than equal to 160 mmHg systolic or greater than or equal to 110 mmHg diastolic within a short interval of minutes) and proteinuria (greater than or equal to 300 mg per 24-hour urine collection either measured or extrapolated, or protein creatinine ratio greater than or equal to 0.3, or a dipstick reading of 1+(pre-2013 guidelines) or 2+(post-2013 guidelines))) or upon new onset hypertension in
- subject can include human or non-human animals.
- methods and described herein are applicable to both human and veterinary disease and animal models.
- Preferred subjects are “patients,” i.e., living humans that are receiving medical care for a disease or condition. This includes persons with no defined illness who are being investigated for signs of pathology.
- preeclampsia can refer to treatments for preeclampsia that would be, statistically speaking, unjustified when a practitioner considers the results of a test or procedure described herein, such as a test based on the determination of an amount of concentration of various protein biomarkers.
- unnecessary treatment can include preterm induction based on one or more symptoms or risk factors for preeclampsia.
- PlGF refers to Placental Growth Factor. PlGF is also referred to as PlGF or PIGF and is encoded by the PGF gene. PlGF includes at least four known isoforms: PlGF-1, PlGF-2, PlGF-3, and PlGF-4.
- sFLT or sFLT1 refers to the soluble ectodomain of Fms-like tyrosine kinase 1, also known as Vascular Endothelial Growth Factor Receptor 1, FLT-1, FLT1, FRT, or VEGFR1.
- free PlGF also “PlGF(f)” or “PlGF-f” refers to PlGF in a biological sample not complexed another other binding partner.
- the other binding partner is sFLT1, with which it can form at least two complexes with PlGF: 2:2 sFLT:PlGF and 2:1 sFLT:PlGF complexes.
- dissociated PlGF refers to PlGF that has been dissociated from its binding partners in a biological sample by subsequent in vitro treatment.
- the other binding partner is sFLT1, with which it can form at least 2:2 sFLT:PlGF and 2:1 sFLT:PlGF complexes.
- dissociate PlGF is equal to “total PlGF” (e.g. the total level of PlGF present in the biological sample).
- the methods, compositions, systems and kits provided herein are used for detecting or predicting a condition in a pregnant human subject at any stage in pregnancy.
- the pregnant human subject is post-the 20 th week of gestation.
- the pregnant human subject is post-first or -second trimester of pregnancy.
- the pregnant human subject is post- or equal to the 13 th , 14 th , 15 th , 16 th , 17 th , 18 th , 19 th , 20 th , 21st, 22nd, 23rd, 24th, 25th, 26th, 27th, 28th, 29th, 30th, 31st, 32nd, 33rd, 34th, 35th, 36th, 37th, 38th, 39th, 40th, 41st, or 42nd week of gestation, or any range between these values.
- preeclampsia preeclampsia
- NonPreE non-preeclampsia
- preeclampsia is further divided into very early-onset (before 25 weeks gestation), early-onset (before 34 weeks gestation) and late-onset (after 34 weeks gestation) preeclampsia.
- the patient is considered NonPreE.
- the patient is generally only suspected to have preeclampsia.
- preeclampsia complications may occur before proteinuria becomes significant
- the clinical presentation of preeclampsia can be highly variable (from severe, rapidly progressing, and early-onset to late-onset and less severe), and the symptoms (hypertension, proteinuria, or both) can be indicative of other distinct disorders that could utilize a different treatment course.
- a subject therefore can be a pregnant female that has no known risk factors or has one or more at-risk factors for a condition such as PreE.
- hypertension and/or proteinuria can indicate a subject at risk of preeclampsia.
- a subject at risk of preeclampsia can have a urine protein content measured as 2+(100 mg/dL) or greater by dipstick assay, greater than or equal to 300 mg per 24-hour urine collection, 30 mg of protein per deciliter or greater in a spot urine sample, or a Pr/Cr ratio in urine of >30 mg per millimole.
- a subject at risk of preeclampsia can have a sitting systolic blood pressure (sSBP) of greater than 140 mmHg or a sitting diastolic blood pressure (sDBP) of greater than 90 mmHg or both.
- sSBP sitting systolic blood pressure
- sDBP sitting diastolic blood pressure
- sFlt1/PlGF ratio can be used to identify subject at risk of preeclampsia (see, e.g., Zeisler et al. NEJM 274(2017):13-22 or Verlohren et al. Hypertension. 63(2014):346-52).
- both hypertension and proteinuria can be used to identify a subject at risk of preeclampsia.
- new onset hypertension in combination with one or more symptoms selected from (a) blood platelet count is less than 100,000 per milliliter, (b) serum creatinine is greater than 1.1 mg/dL or double baseline for the patient, or (c) blood concentration of liver transaminases is twice normal or greater can be used to identify a subject at risk of preeclampsia.
- the molecules are circulating molecules (e.g. unbound to cells and freely circulating in bodily fluids such as blood, blood plasma or blood serum).
- the molecules are expressed in the cytoplasm of blood, endothelial, or organ cells. In some cases, the molecules are expressed on the surface of blood, endothelial, or organ cells.
- a sample can be any material containing tissues, cells, nucleic acids, genes, gene fragments, expression products, proteins, polypeptides, exosomes, gene expression products, or gene expression product fragments of a subject to be tested.
- a sample can include but is not limited to, tissue, cells, plasma, serum, or any other biological material from cells or derived from cells of an individual.
- the sample can be a heterogeneous or homogeneous population of cells or tissues.
- the sample can be a fluid that is acellular or depleted of cells (e.g., plasma or serum).
- the sample is from a single patient.
- the method comprises analyzing multiple samples at once, e.g., via massively parallel multiplex expression analysis on protein arrays or the like.
- the sample is preferably a bodily fluid.
- the bodily fluid can be saliva, urine, blood, and/or amniotic fluid.
- the sample can be a fraction of any of these fluids, such as plasma, serum or exosomes (exemplary exosome isolation techniques can be found, e.g. in Li et al. Theranostics. 7(2017): 789-804).
- the sample is a blood sample, plasma sample, or serum sample.
- the sample may be obtained using any method that can provide a sample suitable for the analytical methods described herein.
- the sample may be obtained by a non-invasive method such as a throat swab, buccal swab, bronchial lavage, urine collection, scraping of the cervix, cervicovaginal sample secretion collection (e.g. with an ophthalmic sponge such as a Weck-Cel sponge), saliva collection, or feces collection.
- the sample may be obtained by a minimally-invasive method such as a blood draw.
- the sample may be obtained by venipuncture.
- obtaining a sample includes obtaining a sample directly or indirectly.
- the sample is taken from the subject by the same party (e.g. a testing laboratory) that subsequently acquires biomarker data from the sample.
- the sample is received (e.g. by a testing laboratory) from another entity that collected it from the subject (e.g. a physician, nurse, phlebotomist, or medical caregiver).
- the sample is taken from the subject by a medical professional under direction of a separate entity (e.g. a testing laboratory) and subsequently provided to said entity (e.g. the testing laboratory).
- the sample is taken by the subject or the subject's caregiver at home and subsequently provided to the party that acquires biomarker data from the sample (e.g. a testing laboratory).
- a testing laboratory e.g. a testing laboratory.
- kits suitable for self or home collection of biological samples have been described commercially and in the literature; see e.g., US20170023446A1 and U.S. Pat. No. 4,777,964A.
- the methods, kits, and systems disclosed herein may comprise data pertaining to one or more samples or uses thereof.
- the data can be representative of an amount or concentration of one or more biomarkers, such as various proteins described herein. Stated differently, the data can be expression level data of proteins or polypeptides.
- the expression level data of biomarkers described herein can be determined by protein array, proteomics, expression proteomics, mass spectrometry (e.g., liquid chromatography-mass spectrometry (LC-MS), multiple reaction monitoring (MRM), selected reaction monitoring (SRM), scheduled MRM, scheduled SRM), 2D PAGE, 3D PAGE, electrophoresis, proteomic chip, proteomic microarray, Edman degradation, direct or indirect ELISA, immunosorbent assay, immuno-PCR (see, e.g., Sano et al. Science. 258(1992):120-2.), proximity extension assay (see, e.g., Thorsen et al. Journal of Translational Medicine.
- mass spectrometry e.g., liquid chromatography-mass spectrometry (LC-MS), multiple reaction monitoring (MRM), selected reaction monitoring (SRM), scheduled MRM, scheduled SRM
- 2D PAGE, 3D PAGE electrophoresis
- proteomic chip e.g.
- Luminex assay or homogenous assays such as ALPHAscreen (see, e.g., Application Note. Nature Methods 5, (2008), U.S. Pat. Nos. 5,898,005A, 5,861,319A), time-resolved fluorescence resonance energy transfer (TR-FRET see e.g., US20130203068A1 and WO1998015830A2), time-resolved fluorescence (TRF), fluorescent oxygen channeling immunoassay (FOCI), or luminescent oxygen channeling immunoassay (LOCITM; see e.g. Ullman et al. Proc Natl Acad Sci USA. 91(1994):5426-5430 or Ullman et al. Clin Chem. 1996 September; 42(9):1518-26 for exemplary methods and reagents).
- ALPHAscreen see, e.g., Application Note. Nature Methods 5, (2008), U.S. Pat. Nos. 5,898,005A, 5,861,319
- compositions, methods and devices described herein make use of labeled molecules in various sandwich, competitive, or non-competitive assay formats to determine expression levels of biomarkers described herein. Such methods generate a signal that is related to the presence or amount of one or more of the proteins described herein. Suitable assay formats also include chromatographic, mass spectrographic, and protein “blotting” methods. Additionally, certain methods and devices, such as biosensors, optical immunoassays, immunosorbent assays, and enzyme immunoassays, may be employed to determine the presence or amount of analytes without the need for a labeled molecule.
- EIA enzyme immunoassays
- ECIA enzyme immunoassays
- ECIA enzyme immunoassays
- ECIA chemiluminescent enzyme immunoassay
- ELIA electrochemiluminescence immunoassay
- ELISA enzyme-linked immunosorbent assay
- Robotic instrumentation for performing these immunoassays are commercially available including, but not limited to Abbott AXSYM®, IMx®, or Commander® systems; Biolog 24i® or CLC480 systems; Beckman Coulter ACCESS®, ACCESS 2®, or Unicel Dxl 600/800 systems; bioMerieux VIDAS® or mini-VIDAS® systems; Chimera Biotec GmbH Imperacer® assay; Dade Behring STRATUS® system; DiaSorin LIAISON XL® or ETI-Max 300 systems; Dynex Agility® system; Gold Standard Diagnostics Thunderbolt® analyzer; Gyrolab xPlore/xPand® system; Hudson Robotics ELISA Workstation; Ortho Clinical Diagnostics Vitros® ECL or 3600 systems; Hamiltorn Robotics ELISA NIMBUS or STARlet systems; Luminex xMAP® system; PerkinElmer ALPHAscreen® or AlphaLISA®; Phadia Laboratory System 100E, 250, 1000, 2
- exemplary analytical systems include assay systems, such as, for example, optical systems containing one or more sources of radiation and/or one more detector. Such systems may use, for example, a light source that illuminates and a sample and a detector configured to detect light that is emitted by the sample (e.g., fluorescence spectroscopy), optical density (e.g., the portion of light that passes through the sample), and/or light that is diffracted by sample (e.g., diffraction optics).
- An analytical system may use, for example, ELISA (enzyme-linked immunosorbent assay).
- An analytical system may use, for example, LOCI (luminescent oxygen channeling), FOCI (fluorescent oxygen channeling), or ALPHAscreen.
- An analytical technique may involve incubating and/or diluting a sample before or during the analysis/assaying of the sample.
- compositions, methods and devices described herein make use of fluorescent oxygen channeling immunoassay (FOCI) compositions and methods.
- FOCI fluorescent oxygen channeling immunoassay
- a first analyte-binding agent that is capable of binding to an analyte and comprises a photosensitizer is used in combination with a second analyte-binding agent comprising a fluorogenic dye.
- the photosensitizer of the first analyte-binding agent generates singlet oxygen in an excited state thereby causing the fluorogenic dye of the second analyte-binding agent to emit fluorescence upon reacting with the singlet oxygen.
- the emitted fluorescence can be detected to, e.g., determine the presence and/or absence of the analyte and/or to quantitate and/or analyze the analyte in a sample.
- the first and the second analyte-binding agents bind to the same region (e.g., epitope) of the analyte (e.g., a protein).
- the first and the second analyte-binding agents comprise the same type of analyte-binding moiety or reagent (e.g., the same antibody).
- the first and the second analyte-binding agents bind to separate regions (e.g., epitopes) of the analyte (e.g., a protein).
- the first and the second analyte-binding agents bind to the separate regions of the analyte (e.g., a protein) that do not spatially overlap.
- the first analyte-binding agent and the second analyte-binding agent are configured such that when both analyte-binding agents are bound to the analyte, the singlet oxygen generated by photosensitizer of the first analyte-binding agent is in close proximity to the fluorogenic dye of the second analyte-binding agent.
- the first and/or second analyte binding agent(s) is an antigen-binding agent (e.g., an antibody).
- the first and/or second analyte binding agent(s) is an affimer.
- the first and/or second analyte binding agent(s) is an antigen-binding agent is an aptamer.
- both the photosensitizer and fluorogenic dye are provided in the form of beads.
- arrays can use different probes (e.g., antibodies, scFvs, Fab fragments) attached to different particles or beads.
- probes e.g., antibodies, scFvs, Fab fragments
- the identity of which probe is attached to which particle or beads may be determinable from an encoding system.
- the probes can be antibodies or antigen-binding fragments or derivatives thereof.
- control samples can be samples from the same patient at different times.
- the one or more control samples can comprise one or more samples from healthy subjects, unhealthy subjects, or a combination thereof.
- the one or more control samples can comprise one or more samples from healthy subjects, subjects suffering from pregnancy-associated conditions other than preeclampsia, subjects suffering chronic conditions along with pregnancy associated conditions, or subjects suffering from chronic conditions alone.
- the expression level data for various samples is used to develop or train an algorithm or classifier provided herein.
- the subject is a patient, such as a pregnant female; gene expression levels are measured in a sample from the patient and a classifier or algorithm (e.g., trained algorithm) is applied to the resulting data in order to detect, predict, monitor, rule out, or estimate the risk of a pregnancy-associated condition such as preeclampsia.
- a classifier or algorithm e.g., trained algorithm
- analysis of expression levels initially provides a measurement of the expression level of each of several individual biomarkers.
- the expression level can be absolute in terms of a concentration of a biomarker, or relative in terms of a relative concentration of an expression product of interest to another biomarker in the sample.
- relative expression levels of proteins can be expressed with respect to the expression level of a house-keeping or structural protein in the sample.
- Relative expression levels can also be determined by simultaneously analyzing differentially labeled samples bound to the same array. Expression levels can also be expressed in arbitrary units, for example, related to signal intensity.
- the classifier is a 2-way classifier.
- a two-way classifier can classify a sample from a pregnant patient into one of two classes comprising preeclampsia (PreE) and non-preeclampsia (nonPreE).
- PreE preeclampsia
- nonPreE non-preeclampsia
- the classifier may be used classify a subject as not needing treatment for preeclampsia.
- a multi-way classifier may be used (e.g., preeclampsia, non-preeclampsia, and indeterminate).
- Classifiers and/or classifier probe sets can be used to either rule-in or rule-out a sample as from a patient to be treated for preeclampsia.
- a classifier can be used to classify a sample as being from a healthy subject.
- a classifier can be used to classify a sample as being from an unhealthy subject.
- classifiers can be used to either rule-in or rule-out a sample as being from a subject who should be treated for preeclampsia.
- supervised learning approaches a group of samples from two or more groups (e.g. PE and non-PE) are analyzed with a statistical classification method. Biomarker presence/level data can be used as a classifier that differentiates between the two or more groups. A new sample can then be analyzed so that the classifier can associate the new sample with one of the two or more groups.
- Commonly used supervised classifiers include without limitation the neural network (multi-layer perceptron), support vector machines, k-nearest neighbors, Gaussian mixture model, Gaussian, naive Bayes, decision tree and radial basis function (RBF) classifiers.
- Linear classification methods include Fisher's linear discriminant, logistic regression, naive Bayes classifier, perceptron, and support vector machines (SVMs).
- Other classifiers for use with the invention include quadratic classifiers, k-nearest neighbor, boosting, decision trees, random forests, neural networks, pattern recognition, Bayesian networks and Hidden Markov models.
- quadratic classifiers include quadratic classifiers, k-nearest neighbor, boosting, decision trees, random forests, neural networks, pattern recognition, Bayesian networks and Hidden Markov models.
- Classification using supervised methods is generally performed by the following methodology:
- a training set can include, for example, serum samples from pregnant female patients (e.g. PE and non-PE). The training samples are used to “train” the classifier.
- the accuracy of the learned function depends on how the input object is represented.
- the input object is transformed into a feature vector, which contains a number of features that are descriptive of the object.
- the number of features should not be too large, because of the curse of dimensionality; but should be large enough to accurately predict the output.
- the features might include a set of biomarkers as described herein.
- a learning algorithm (e.g. “trained algorithm”) is chosen, e.g., artificial neural networks, decision trees, Bayes classifiers or support vector machines. The learning algorithm is used to build the classifier.
- the learning algorithm is run on the gathered training set. Parameters of the learning algorithm may be adjusted by optimizing performance on a subset (called a validation set) of the training set, or via cross-validation. After parameter adjustment and learning, the performance of the algorithm may be measured on a test set of naive samples that is separate from the training set.
- a validation set a subset of the training set
- the classifier e.g. classification model
- a sample e.g., a serum sample from a pregnant patient that is being analyzed by the methods described herein.
- Clustering is an unsupervised learning approach wherein a clustering algorithm correlates a series of samples without the use the labels. The most similar samples are sorted into “clusters.” A new sample could be sorted into a cluster and thereby classified with other members that it most closely associates.
- the methods, kits, and systems disclosed herein can comprise algorithms or uses thereof.
- the one or more algorithms can be used to classify one or more samples from one or more subjects.
- the one or more algorithms can be applied to data from one or more samples.
- the data can comprise biomarker expression data.
- the methods disclosed herein can comprise assigning a classification to one or more samples from one or more subjects. Assigning the classification to the sample can comprise applying an algorithm to the expression level.
- the gene expression levels are inputted to a data analysis system comprising a trained algorithm for classifying the sample as one of the conditions comprising preeclampsia, eclampsia, non-preeclampsia, chronic hypertension, gestational hypertension, or HELLP (Hemolysis, Elevated Liver enzymes, and Low Platelet count—see e.g., Weinstein et al. Am J Obstet Gynecol. 142(1982):159-67) syndrome.
- the algorithm can, as part of its execution, calculate an index for a sample and compare the sample index to a threshold value; the predefined relationship can be indicative of a likelihood of the sample belonging to a particular classification.
- the algorithm can provide a record of its output including a classification of a sample and/or a confidence level.
- the output of the algorithm can be the possibility of the subject of having a condition comprising preeclampsia, eclampsia, chronic hypertension, gestational hypertension, or HELLP syndrome.
- a data analysis system can be a trained algorithm.
- the algorithm can comprise a linear classifier.
- the linear classifier comprises one or more of linear discriminant analysis, Fisher's linear discriminant, Na ⁇ ve Bayes classifier, Logistic regression, Perceptron, Support vector machine, or a combination thereof.
- the linear classifier can be a support vector machine (SVM) algorithm.
- the algorithm can comprise a two-way classifier.
- the two-way classifier can comprise one or more decision tree, random forest, Bayesian network, support vector machine, neural network, or logistic regression algorithms.
- the algorithm can comprise one or more linear discriminant analysis (LDA), Basic perceptron, Elastic Net, logistic regression, (Kernel) Support Vector Machines (SVM), Diagonal Linear Discriminant Analysis (DLDA), Golub Classifier, Parzen-based, (kernel) Fisher Discriminant Classifier, k-nearest neighbor, Iterative RELIEF, Classification Tree, Maximum Likelihood Classifier, Random Forest, Nearest Centroid, Prediction Analysis of Microarrays (PAM), k-medians clustering, Fuzzy C-Means Clustering, Gaussian mixture models, graded response (GR), Gradient Boosting Method (GBM), Elastic-net logistic regression, logistic regression, or a combination thereof.
- LDA linear discriminant analysis
- SVM Support Vector Machines
- DLDA Diagonal Linear Discriminant Analysis
- Golub Classifier Parzen-based
- (kernel) Fisher Discriminant Classifier k-nearest neighbor
- Iterative RELIEF Classification Tree
- the algorithm can comprise a Diagonal Linear Discriminant Analysis (DLDA) algorithm.
- the algorithm can comprise a Nearest Centroid algorithm.
- the algorithm can comprise a Random Forest algorithm.
- GBM gradient boosting method for discrimination of preeclampsia and non-preeclampsia
- LDA linear discriminant analysis
- SVM support vector machine
- biomarker refers to a measurable indicator of some biological state or condition.
- a biomarker can be a substance found in a subject, a quantity of the substance, or some other indicator.
- a biomarker can be the amount of a protein and/or other gene expression products in a sample.
- a biomarker is a full-length, unmodified protein.
- a biomarker is an alternatively spliced, post-translationally cleaved, or post-translationally chemically modified (e.g., methylated, phosphorylated, glycosylated, formylated, etc) protein.
- a biomarker is a complex of a protein with another protein at a particular stoichiometry.
- a biomarker is a free protein in the absence of other associated binding partners.
- the methods, compositions and systems as described here also relate to the use of biomarker panels and/or gene expression products for purposes of identification, diagnosis, classification, treatment or to otherwise characterize various conditions of pregnant patients comprising NonPreE, PreE, chronic hypertension, gestational hypertension, or HELLP syndrome.
- Sets of biomarkers and/or gene expression products useful for classifying biological samples are provided, as well as methods of obtaining such sets of biomarkers.
- the pattern of levels of gene expression biomarkers in a panel (also known as a signature) is determined from one or more references samples and then used to evaluate the signature of the same panel of biomarkers in a test sample, such as by a measure of similarity between the test sample signature and the reference sample signature.
- the methods provided herein can comprise identifying or ruling out a condition from a combination of a species of PlGF with secondary biomarkers.
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) one or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the methods provided herein can comprise identifying or ruling out a condition (e.g.
- preeclampsia from (e.g., based on analysis from) one or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- preeclampsia is thought to originate in abnormal trophoblast invasion, which results in incomplete spiral artery remodeling and hypoperfusion of the placenta, and that this hypoperfusion of the placenta triggers dysfunction in multiple body systems causing the signs and symptoms of preeclampsia.
- dysfunctional systems can include, as a result of placental hypoperfusion, angiogenesis and endothelial function, as evidenced e.g. by imbalances in pro-and-anti-angiogenic factors, many of which are released by the placenta in response to the abnormal physiology of preeclampsia, and which disrupt vascular homeostasis in the mother's body.
- SFLT1 Soluble FMS-like tyrosine kinase 1, a tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PlGF and decreases towards term, and plays an essential role in the development of embryonic vasculature
- PlGF Placental growth factor, a proangiogenic protein peaking at 30 weeks of gestation that stimulates endothelial cell growth, proliferation, and migration
- DCN Decorin, which is a functional component of the extracellular matrix and plays a role in tissue repair and regulation of cell adhesion and migration by binding to ECM molecules
- ENG Enddoglin, which in its soluble form, sENG (Endoglin) is a powerful antiangiogenic molecule, and acts by inhibiting TGF-â1 binding
- FGF21 Fibroblast growth factor 21, which has been demonstrated to be expressed in placental syncytiotrophoblasts, and is both an adipokine and
- KIM-1 Kidney Injury Molecule-1
- KIdney Injury Molecule-1 is considered to be a marker of dysfunction in oxygen signaling in preeclampsia, as its expression is known to increase in response to local hypoxia/ischemia in proximal renal tubule cells.
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1 or Endoglin; and (b) PlGF (free, dissociated, or % free).
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1 or Endoglin; (b) PlGF (free, dissociated, or % free); and (c) KIM1.
- the methods provided herein can comprise identifying or ruling out a condition (e.g.
- preeclampsia from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1 or Endoglin; (b) PlGF (free, dissociated, or % free); (c) KIM1; and (d) FGF21.
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1 or Endoglin; (b) PlGF (free, dissociated, or % free); (c) KIM1; and (d) Decorin.
- the methods provided herein can comprise identifying or ruling out a condition (e.g.
- preeclampsia from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1 or Endoglin; (b) PlGF (free, dissociated, or % free); (c) KIM1; (d) FGF21; and (e) Decorin.
- the biomarkers may comprise both sFLT1 and Endoglin.
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1; (b) Endoglin; and (c) PlGF (free, dissociated, or % free).
- the methods provided herein can comprise identifying or ruling out a condition (e.g.
- preeclampsia from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1; (b) Endoglin; (c) PlGF (free, dissociated, or % free); and (d) KIM1.
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1; (b) Endoglin; (c) PlGF (free, dissociated, or % free); (d) KIM1; and (e) FGF21.
- the methods provided herein can comprise identifying or ruling out a condition (e.g.
- preeclampsia from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1; (b) Endoglin; (c) PlGF (free, dissociated, or % free); (d) KIM1; and (e) Decorin.
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1; (b) Endoglin; (c) PlGF (free, dissociated, or % free); (d) KIM1; (e) FGF21; and (f) Decorin.
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) two or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- a condition e.g. preeclampsia
- the methods provided herein can comprise identifying or ruling out a condition (e.g.
- preeclampsia from (e.g., based on analysis from) three or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) four or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) five or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- a condition e.g. preeclampsia
- the methods provided herein can comprise identifying or ruling out a condition (e.g.
- preeclampsia from (e.g., based on analysis from) six or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) seven or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) all of sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- a condition e.g. preeclampsia
- the methods provided herein can comprise identifying or ruling out a condition (e.g.
- preeclampsia from (e.g., based on analysis from) two biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) three biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) four biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- a condition e.g. preeclampsia
- the methods provided herein can comprise identifying or ruling out a condition (e.g.
- preeclampsia from (e.g., based on analysis from) five biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) six biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) seven biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- a condition e.g. preeclampsia
- the methods provided herein can comprise identifying or ruling out a condition (e.g.
- preeclampsia from (e.g., based on analysis from) no more than two biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) no more than three biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) no more than four biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- a condition e.g. preeclampsia
- the methods provided herein can comprise identifying or ruling out a condition (e.g.
- preeclampsia from (e.g., based on analysis from) no more than five biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) no more than six biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) no more than seven biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- a condition e.g. preeclampsia
- biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the methods, compositions, systems and kits provided herein can be used to detect, diagnose, predict or monitor a condition of a pregnant patient.
- the methods, compositions, systems and kits described herein provide information to a medical practitioner that can be useful in making a clinical therapeutic decision.
- Clinical and therapeutic decisions can include decisions to: continue with a particular therapy, modify a particular therapy, alter the dosage of a particular therapy, stop or terminate a particular therapy, altering the frequency of a therapy, introduce a new therapy, introduce a new therapy to be used in combination with a current therapy, or any combination of the above.
- medical action taken may comprise watchful waiting or the administration of one or more additional diagnostic tests of the same or different nature.
- a clinical decision may be made to not induce labor, or to proceed with ambulant monitoring of the subject.
- the methods provided herein can be applied in an experimental setting, e.g., clinical trial.
- the methods provided herein can be used to monitor a pregnant patient who is being treated with an experimental agent such as an angiogenic/antiangiogenic drug, compound, or therapeutic cell type.
- the methods provided herein can be useful to determine whether a subject can be administered an experimental agent (e.g., an agonist, antagonist, peptidomimetic, protein, peptide, nucleic acid, therapeutic cell, small molecule, or other drug candidate) to reduce the risk of preeclampsia.
- an experimental agent e.g., an agonist, antagonist, peptidomimetic, protein, peptide, nucleic acid, therapeutic cell, small molecule, or other drug candidate
- the methods described herein can be useful in determining if a subject can be effectively treated with an experimental agent and for monitoring the subject for risk of preeclamp
- the methods, compositions, systems and kits provided herein are particularly useful for detecting, diagnosing, or ruling out a condition of a pregnant patient such as a condition the pregnant patient has at the time of testing.
- An exemplary condition that can be detected, diagnosed, or ruled out with the present method includes preeclampsia.
- the methods, compositions, systems, and kits provided herein can also be useful, in combination with other standard clinical data collected for pregnant patients, for ruling in or ruling out a diagnosis of preeclampsia, hypertension, gestational hypertension, or HELLP syndrome.
- the methods provided herein are particularly useful for pregnant patients who have exhibited one or more new-onset symptoms associated with preeclampsia prior to testing (e.g., hypertension, proteinuria, low platelet count, elevated serum creatinine levels, elevated liver enzymes, pulmonary edema, or cerebral/visual symptoms), such that the patients are suspected of having preeclampsia.
- preeclampsia e.g., hypertension, proteinuria, low platelet count, elevated serum creatinine levels, elevated liver enzymes, pulmonary edema, or cerebral/visual symptoms
- the methods, compositions, systems and kits provided herein can rule out a diagnosis of preeclampsia for a specified number of days in the future.
- the specified number of days in the future is 1 to 30 days.
- the specified number of days in the future is at least 1 day.
- the specified number of days in the future is at most 30 days.
- the specified number of days in the future is 1 day to 5 days, 1 day to 10 days, 1 day to 30 days, 5 days to 10 days, 5 days to 30 days, or 10 days to 30 days.
- the specified number of days in the future is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 days.
- the specified number of days in the future is 5 days to 10 days. In other preferred embodiments, the specified number of days in the future is 5, 6, 7, 8, 9, or 10 days.
- the methods, compositions, systems and kits provided herein can rule-out mothers for hospital admission and preterm delivery. In some embodiments, the methods, compositions, systems and kits provided herein can rule out a diagnosis of preeclampsia for a specified number of weeks in the future. In some instances, the specified number of weeks is at least 1 week. In some instances, the specified number of weeks is at least 2 weeks. In some instances, the specified number of weeks is at least 3 weeks. In some instances, the specified number of weeks is at least 4 weeks. In some instances, the specified number of weeks is at least 5 weeks.
- the specified number of weeks is at least 6 weeks. In some instances, the specified number of weeks is at most 1 week. In some instances, the specified number of weeks is at most 2 weeks. In some instances, the specified number of weeks is at most 3 weeks. In some instances, the specified number of weeks is at most 4 weeks. In some instances, the specified number of weeks is at most 5 weeks. In some instances, the specified number of weeks is at most 6 weeks.
- the methods, compositions, systems and kits provided herein can rule out a diagnosis of preeclampsia for a specified number of weeks in the future with a particular PPV.
- the positive predictive value (PPV) is at least about 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, or 57%, or any range in between these values.
- the methods, compositions, systems and kits provided herein can rule out a diagnosis of preeclampsia for a specified number of weeks in the future with a particular NPV.
- the NPV can be at least about 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 95.2%, 95.5%, 95.7%, 96%, 96.2%, 96.5%, 96.7%, 97%, 97.2%, 97.5%, 97.7%, 98%, 98.2%, 98.5%, 98.7%, 99%, 99.2%, 99.5%, 99.7%, or 99.9%, or any range in between these values.
- the methods, compositions, systems and kits provided herein can rule out a diagnosis of preeclampsia for a specified number of weeks in the future with a particular AUC.
- the AUC of can be at least about 50%, 53%, 55%, 57%, 60%, 63%, 65%, 67%, 70%, 72%, 75%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, or any range in between these values.
- the methods, compositions, systems and kits provided herein can rule out a diagnosis of preeclampsia for a specified number of weeks in the future with a particular AUP.
- the AUP can be at least about 50%, 53%, 55%, 57%, 60%, 63%, 65%, 67%, 70%, 72%, 75%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, or any range in between these values.
- the diagnosis, detection, or ruling out of a condition of the pregnant patient can be particularly useful in limiting the number of unnecessary invasive medical interventions that are administered to the patient, and/or indicating alternative less-invasive therapeutic interventions such as pharmacological therapies (anticonvulsants, antihypertensives, central alpha agonists, alpha-blockers, beta-blockers, calcium-channel blockers, vasodilators, cyclooxygenase inhibitors, or, acetylsalicylic acid).
- pharmacological therapies anticonvulsants, antihypertensives, central alpha agonists, alpha-blockers, beta-blockers, calcium-channel blockers, vasodilators, cyclooxygenase inhibitors, or, acetylsalicylic acid.
- the methods provided herein can limit, delay, or eliminate the use of preterm cesarean delivery or labor induction in patients suspected of having preeclampsia via high-confidence ruling out of a diagnosis of preeclampsia in the pregnant patient (e.g., via a high negative predictive value of the methods, compositions, systems, and kits provided herein).
- the methods provided herein can inform the clinical decision to proceed to delivery (e.g. when the pregnant patient is identified as being at-risk of preeclampsia).
- a decision may involve the administration of a labor-inducing or cervical ripening agent (e.g. prostaglandin, oxytocin, misoprostol, mifepristone, or relaxin), a corticosteroid (to aid in lung development prior to delivery), and/or performing a C-section.
- a labor-inducing or cervical ripening agent e.g. prostaglandin, oxytocin, misoprostol, mifepristone, or relaxin
- a corticosteroid to aid in lung development prior to delivery
- the methods, compositions, systems and kits provided herein can be used alone or in combination with other standard diagnosis methods currently used to detect, diagnose, or rule out a condition of a pregnant patient, such as but not limited to blood pressure measurement, urine protein measurement, blood platelet counting, serum creatinine level measurement, creatinine clearance measurement, urine protein/creatinine ratio measurement, serum transaminase level measurement, serum LDH level measurement, serum bilirubin level measurement, or Doppler ultrasound indices (e.g., uterine artery indices).
- other standard diagnosis methods currently used to detect, diagnose, or rule out a condition of a pregnant patient such as but not limited to blood pressure measurement, urine protein measurement, blood platelet counting, serum creatinine level measurement, creatinine clearance measurement, urine protein/creatinine ratio measurement, serum transaminase level measurement, serum LDH level measurement, serum bilirubin level measurement, or Doppler ultrasound indices (e.g., uterine artery indices).
- hypertension in a pregnant patient can be indicative of conditions such as chronic hypertension, gestational hypertension, or preeclampsia; ruling out the diagnosis of preeclampsia via the methods, compositions, systems and kits provided herein allows for the patient to be correctly diagnosed with chronic hypertension or gestational hypertension.
- the methods provided herein can predict preeclampsia prior to actual onset of the condition or symptoms associated with the condition (e.g., hypertension or proteinuria). In some instances, the methods provided herein can predict preeclampsia or other disorders in a pregnant patient at least 1 day, 1 week, 2 weeks, 3 weeks, 1 month, 2 months prior to onset of the condition or symptoms associated with the condition. In other instances, the methods provided herein can predict preeclampsia or other disorders in a pregnant patient at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or 31 days prior to onset. In other instances, the methods provided herein can predict preeclampsia or other disorders in a pregnant patient at least 1, 2, 3, or 4 months prior to onset.
- the monitoring is conducted by serial testing, such as serial non-invasive tests, serial minimally-invasive tests (e.g., blood draws), or some combination thereof.
- serial non-invasive tests e.g., blood draws
- serial minimally-invasive tests e.g., blood draws
- the monitoring is conducted by administering serial non-invasive tests or serial minimally-invasive tests (e.g., blood draws).
- the pregnant patient is monitored as needed (e.g., on an as-needed basis) using the methods described herein. Additionally, or alternatively the pregnant patient can be monitored weekly, monthly, or at any pre-specified intervals. In some instances, the pregnant patient is monitored at least once every 24 hours. In some instances, the pregnant patient is monitored at least once every 1 day to 30 days. In some instances, the pregnant patient is monitored at least once every at least 1 day. In some instances, the pregnant patient is monitored at least once every at most 30 days.
- the pregnant patient is monitored at least (optionally on average) once every 1 day to 5 days, 1 day to 10 days, 1 day to 15 days, 1 day to 20 days, 1 day to 25 days, 1 day to 30 days, 5 days to 10 days, 5 days to 15 days, 5 days to 20 days, 5 days to 25 days, 5 days to 30 days, 10 days to 15 days, 10 days to 20 days, 10 days to 25 days, 10 days to 30 days, 15 days to 20 days, 15 days to 25 days, 15 days to 30 days, 20 days to 25 days, 20 days to 30 days, or 25 days to 30 days.
- the pregnant patient is monitored at least once every 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 28, 29, 30 or 31 days.
- the pregnant patient is monitored at least once every 1, 2, or 3 months. In some instances, the pregnant patient is monitored via the methods described herein no more frequently than one week, 10 days, two weeks, three weeks, or one month. In other words, the predictive value of the some of the methods described herein can be of clinical use for at least one week, at least 10 days, at least two weeks, at least three weeks, or at least one month.
- biomarker expression levels in the patients can be measured, for example, within, one week, two weeks, three weeks, or four weeks after detection of one or more symptoms associated with preeclampsia (e.g., hypertension or proteinuria).
- biomarker expression levels are determined at regular intervals, e.g., every 1 week, 2 weeks, 3 weeks, 1 month, 2 months or 3 months post-conception, after the beginning of the 2 nd trimester, after the beginning of the 3 rd trimester, or after week 20 of the pregnancy, either indefinitely, or until evidence of a condition is observed.
- biomarker expression levels are determined at regular intervals after week 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, or 42 weeks. Where evidence of a condition is observed, the frequency of monitoring is sometimes increased.
- baseline values of expression levels are determined in a subject before detection of one or more symptoms associated with preeclampsia (e.g., hypertension or proteinuria) in combination with determining expression levels after onset of symptoms.
- the results of diagnosing, predicting, ruling out, or monitoring a condition of the pregnant patient can be useful for informing a clinical or therapeutic decision such as determining or monitoring a therapeutic regimen.
- an entity that acquires sample data and/or classifies a sample from a patient as having preeclampsia is other than the physician, caregiver, or medical institution performing the treatment.
- the entity acquiring sample data e.g. levels of levels of one or more biomarkers described herein
- calculating an index based (at least in part) on the levels of the plurality of the protein biomarkers, and/or determining risk of preeclampsia is a third-party testing service.
- determining or monitoring a therapeutic regimen first comprises receiving information from a third-party testing service, which can comprise, for example (but not limited to), classification of a sample as being at risk or not of preeclampsia, risk of a pregnant patient having preeclampsia, levels of a plurality of protein biomarkers from the sample associated with preeclampsia (e e.g. levels of levels of one or more biomarkers described herein), or the likelihood a pregnant patient will deliver preterm.
- a third-party testing service can comprise, for example (but not limited to), classification of a sample as being at risk or not of preeclampsia, risk of a pregnant patient having preeclampsia, levels of a plurality of protein biomarkers from the sample associated with preeclampsia (e e.g. levels of levels of one or more biomarkers described herein), or the likelihood a pregnant patient will deliver preterm.
- an entity that acquires sample data, determines the risk of preterm birth of the patient from the sample, and/or classifies a sample from a patient as having a significant risk of preterm birth is the same entity that performing the treatment.
- determining a therapeutic regimen can comprise administering a therapeutic drug. In some instances, determining a therapeutic regimen comprises modifying, continuing, initiating or stopping a therapeutic regimen. In some instances, determining a therapeutic regimen comprises treating the disease or condition (e.g., preeclampsia, eclampsia, gestational hypertension, hypertension, or HELLP syndrome). In some instances, the therapy is an anti-hypertensive therapy. In some instances, the therapy is an anti-cyclooxygenase (COX) therapy. In some instances, the therapy is an anti-convulsant therapy.
- COX anti-cyclooxygenase
- Modifying the therapeutic regimen can comprise terminating a therapy. Modifying the therapeutic regimen can comprise altering a dosage of a therapy. Modifying the therapeutic regimen can comprise altering a frequency of a therapy. Modifying the therapeutic regimen can comprise administering a different therapy.
- the results of diagnosing, predicting, or monitoring a condition of the pregnant patient can be useful for informing a therapeutic decision such as caesarean delivery.
- Other examples of therapeutic decisions can be cervical ripening and/or labor induction. Examples of agents that can be used for cervical ripening and/or labor induction include prostaglandins, misoprostol, mifepristone, relaxin, and oxytocin. Other examples of therapeutic decisions can be cesarean delivery.
- Examples of a therapeutic regimen can include administering compounds or agents having anti-hypertensive properties (e.g., central alpha agonists such as methyldopa, vasodilators such as clonidine, diazoxide, hydralazine and prazosin, calcium-channel blockers such as nifedipine and verapamil, alpha-blockers such as labetalol, or beta-blockers such as oxprenolol), compounds or agents having anti-cyclooxygenase activity (e.g., acetylsalicylic acid), or compounds having anti-convulsant activity (e.g., phenytoin or magnesium sulfate). These compounds can be used alone or in combination.
- compounds or agents having anti-hypertensive properties e.g., central alpha agonists such as methyldopa, vasodilators such as clonidine, diazoxide, hydralazine and
- modifying the therapeutic regimen can comprise proceeding with treatment of said pregnant human in a manner that avoids unnecessary treatment of preeclampsia.
- managing the pregnant human subject identified not as at risk for preeclampsia comprises ambulant monitoring, or refraining from the administration of any drug for treating preeclampsia.
- antihypertensive drugs (rather than delivery) may be prescribed and/or administered to the patient.
- the preeclampsia test is repeated at an increased frequency.
- the test can be re-performed, and sample re-drawn from the patient weekly, monthly, or at any pre-specified intervals.
- the pregnant patient is monitored at least once every 24 hours. In some instances, the pregnant patient is monitored at least once every 1 day to 30 days. In some instances, the pregnant patient is monitored at least once every at least 1 day. In some instances, the pregnant patient is monitored at least once every at most 30 days.
- the pregnant patient is monitored at least (optionally on average) once every 1 day to 5 days, 1 day to 10 days, 1 day to 15 days, 1 day to 20 days, 1 day to 25 days, 1 day to 30 days, 5 days to 10 days, 5 days to 15 days, 5 days to 20 days, 5 days to 25 days, 5 days to 30 days, 10 days to 15 days, 10 days to 20 days, 10 days to 25 days, 10 days to 30 days, 15 days to 20 days, 15 days to 25 days, 15 days to 30 days, 20 days to 25 days, 20 days to 30 days, or 25 days to 30 days.
- the pregnant patient is monitored at least once every 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 28, 29, 30 or 31 days.
- the pregnant patient is monitored at least once every 1, 2, or 3 months. In some instances, the pregnant patient is monitored via the methods described herein no more frequently than one week, 10 days, two weeks, three weeks, or one month. In other words, the predictive value of the some of the methods described herein can be of clinical use for at least one week, at least 10 days, at least two weeks, at least three weeks, or at least one month.
- the methods, kits, and systems disclosed herein for use in identifying, classifying (or ruling out a classification) or characterizing a sample can be characterized by having a specificity of at least about 80% using the methods disclosed herein. In some embodiments, the specificity of the methods is at least about 85%. In some embodiments, the specificity of the methods is at least about 90%. In some embodiments, the specificity of the methods is at least about 95%. The specificity of the method can be at least about 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%, or any range in between these values.
- the present invention provides a method of identifying, classifying (or ruling out a classification) or characterizing a sample that gives a sensitivity of at least about 80% using the methods disclosed herein.
- the sensitivity of the methods is at least 85%.
- the sensitivity of the methods is at least 90%.
- the sensitivity of the methods is at least 95%.
- the sensitivity of the method can be at least about 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%, or any range in between these values.
- the methods, kits and systems disclosed herein can improve upon the accuracy of current methods of monitoring or predicting a status or outcome of a pregnancy (e.g. preeclampsia) or identifying or ruling out a classification of a sample.
- the accuracy of the methods, kits, and systems disclosed herein can be at least about 50%, 53%, 55%, 57%, 60%, 63%, 65%, 67%, 70%, 72%, 75%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, or any range in between these values.
- the methods, kits, and systems for use in identifying, classifying (or ruling out a classification) or characterizing a sample can be characterized by having a negative predictive value (NPV) greater than or equal to 90%.
- the NPV can be at least about 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 95.2%, 95.5%, 95.7%, 96%, 96.2%, 96.5%, 96.7%, 97%, 97.2%, 97.5%, 97.7%, 98%, 98.2%, 98.5%, 98.7%, 99%, 99.2%, 99.5%, 99.7%, or 99.9%, or any range in between these values.
- the NPV can be greater than or equal to 95%.
- the NPV can be greater than or equal to 96%.
- the NPV can be greater than or equal to 97%.
- the methods, kits, and/or systems disclosed herein for use in identifying, classifying (or ruling out a classification) or characterizing a sample can be characterized by having a positive predictive value (PPV) of at least about 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, or 57%, or any range in between these values using the methods disclosed herein.
- PSV positive predictive value
- the methods, kits and systems disclosed herein can improve upon the AUC of current methods of monitoring or predicting a status or outcome of a pregnancy (e.g. preeclampsia) or identifying or ruling out a classification of a sample.
- the AUC of the methods, kits, and systems disclosed herein can be at least about 50%, 53%, 55%, 57%, 60%, 63%, 65%, 67%, 70%, 72%, 75%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, or any range in between these values.
- the methods, kits and systems disclosed herein can improve upon the AUP of current methods of monitoring or predicting a status or outcome of a pregnancy (e.g. preeclampsia) or identifying or ruling out a classification of a sample.
- the AUP of the methods, kits, and systems disclosed herein can be at least about 50%, 53%, 55%, 57%, 60%, 63%, 65%, 67%, 70%, 72%, 75%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, or any range in between these values.
- the methods, kits, and systems disclosed herein for use in diagnosing, prognosing, and/or monitoring a status or outcome of a pregnancy in a subject in need thereof can be characterized by having an accuracy of at least about 80%, 82%, 85%, 87%, 90%, 92%, 95%, or 97% or any range in between these values.
- the methods, kits, and systems disclosed herein for use in diagnosing, prognosing, and/or monitoring a status or outcome of a pregnancy in a subject in need thereof can be characterized by having a specificity of at least about 80%, 82%, 85%, 87%, 90%, 92%, 95%, or 97%, or any range in between these values.
- the methods, kits, and systems disclosed herein for use in diagnosing, prognosing, and/or monitoring a status or outcome of a pregnancy in a subject in need thereof can be characterized by having a sensitivity of at least about 80%, 82%, 85%, 87%, 90%, 92%, 95%, or 97%, or any range in between these values.
- the methods, kits, and systems disclosed herein for use in diagnosing, prognosing, and/or monitoring a status or outcome of a pregnancy in a subject in need thereof can be characterized by having a negative predictive value (NPV) greater than or equal to 90%.
- the NPV can be at least about 90%, 91%, 92%, 93%, 94%, 95%, 95.2%, 95.5%, 95.7%, 96%, 96.2%, 96.5%, 96.7%, 97%, 97.2%, 97.5%, 97.7%, 98%, 98.2%, 98.5%, 98.7%, 99%, 99.2%, 99.5%, 99.7%, or 99.9%, or any range in between these values.
- the NPV can be greater than or equal to 95%.
- the NPV can be greater than or equal to 96%.
- the NPV can be greater than or equal to 97%.
- the NPV can be greater than or equal to 98%.
- the methods, kits, and systems disclosed herein for use in diagnosing, prognosing, and/or monitoring a status or outcome of a pregnancy in a subject in need thereof can be characterized by having a positive predictive value (PPV) of at least about 80%.
- the methods, kits, and systems disclosed herein for use in diagnosing, prognosing, and/or monitoring a status or outcome of a pregnancy in a subject in need thereof can be characterized by having a positive predictive value (PPV) of at least about 85%.
- the methods, kits, and systems disclosed herein for use in diagnosing, prognosing, and/or monitoring a status or outcome of a pregnancy in a subject in need thereof can be characterized by having a positive predictive value (PPV) of at least about 90%.
- the PPV can be at least about 80%, 85%, 90%, 95%, 95.2%, 95.5%, 95.7%, 96%, 96.2%, 96.5%, 96.7%, 97%, 97.2%, 97.5%, 97.7%, 98%, 98.2%, 98.5%, 98.7%, 99%, 99.2%, 99.5%, 99.7%, or 99.9%, or any range in between these values.
- the PPV can be greater than or equal to 95%.
- the PPV can be greater than or equal to 98%.
- disclosure provides a test for confirming preeclampsia in a subject, preferably a pregnant subject, wherein the test is able to discern subjects not having preeclampsia but having one or more symptoms associated with preeclampsia from subjects having by preeclampsia with an NPV of at least about 90%, 91%, 92%, 93%, 94%, 95%, 95.2%, 95.5%, 95.7%, 96%, 96.2%, 96.5%, 96.7%, 97%, 97.2%, 97.5%, 97.7%, 98%, 98.2%, 98.5%, 98.7%, 99%, 99.2%, 99.5%, 99.7%, or 99.9%, or any range in between these values.
- the one or more symptoms associated with preeclampsia can be diabetes (e.g. gestational, type I or type II), higher than normal glucose level, hypertension (e.g. chronic or non-chronic), excessive or sudden weight gain, higher than normal weight, obesity, higher than normal body mass index (BMI), abnormal weight gain, abnormal blood pressure, water retention, hereditary factors, abnormal proteinuria, headache, edema, abnormal protein/creatinine ratio, abnormal platelet count, stress, nulliparity, abnormal Papanicolaou test results (Pap smear), prior preeclampsia episodes (e.g., personal history of PreE), familial history of preeclampsia, preeclampsia in prior pregnancies, renal disease, thrombophilia, or any combination thereof. Gestational age may also be used in tests, such as tests for ruling out preeclampsia.
- diabetes e.g. gestational, type I or type II
- hypertension e.g. chronic
- a computer program can include a sequence of instructions, executable in the digital processing device's CPU (i.e. processor), written to perform a specified task.
- Computer readable instructions can be implemented as program modules, such as functions, objects, Application Programming Interfaces (APIs), data structures, and the like, that perform particular tasks or implement particular abstract data types.
- APIs Application Programming Interfaces
- a computer program can be written in various versions of various languages.
- the functionality of the computer readable instructions can be combined or distributed as desired in various environments.
- the computer program will normally provide a sequence of instructions from one location or a plurality of locations.
- the system can comprise (a) a digital processing device comprising an operating system configured to perform executable instructions and a memory device; (b) a computer program including instructions executable by the digital processing device to classify a sample from a subject comprising: (i) a first software module configured to receive a biomarker expression profile of one or more biomarkers from the sample from the subject; (ii) a second software module configured to analyze the biomarker expression profile from the subject; and (iii) a third software module configured to classify the sample from the subject based on a classification system.
- the classification system comprises two classes. In other embodiments, the classification system comprises two or more classes.
- At least two of the classes can be selected from preeclampsia, non-preeclampsia (e.g., for at least a period of time), normal pregnancy, complicated pregnancy, and gestational hypertension.
- Analyzing the biomarker expression profile from the subject can comprise applying an algorithm.
- Analyzing the biomarker expression profile can comprise normalizing the biomarker expression profile from the subject.
- FIG. 11 shows a computer system (also “system” herein) 401 programmed or otherwise configured for implementing the methods of the disclosure, such as producing a selector set and/or for data analysis.
- the system 401 includes a central processing unit (CPU, also “processor” and “computer processor” herein) 405 , which can be a single core or multi core processor, or a plurality of processors for parallel processing.
- the system 401 also includes memory 410 (e.g., random-access memory, read-only memory, flash memory), electronic storage unit 415 (e.g., hard disk), communications interface 420 (e.g., network adapter) for communicating with one or more other systems, and peripheral devices 425 , such as cache, other memory, data storage and/or electronic display adapters.
- memory 410 e.g., random-access memory, read-only memory, flash memory
- electronic storage unit 415 e.g., hard disk
- communications interface 420 e.g., network adapter
- peripheral devices 425
- the memory 410 , storage unit 415 , interface 420 and peripheral devices 425 are in communication with the CPU 405 through a communications bus (solid lines), such as a motherboard.
- the storage unit 415 can be a data storage unit (or data repository) for storing data.
- the system 401 is operatively coupled to a computer network (“network”) 430 with the aid of the communications interface 420 .
- the network 430 can be the Internet, an internet and/or extranet, or an intranet and/or extranet that is in communication with the Internet.
- the network 430 in some instances is a telecommunication and/or data network.
- the network 430 can include one or more computer servers, which can enable distributed computing, such as cloud computing.
- the network 430 in some instances, with the aid of the system 401 , can implement a peer-to-peer network, which can enable devices coupled to the system 401 to behave as a client or a server.
- the system 401 is in communication with a processing system 435 .
- the processing system 435 can be configured to implement the methods disclosed herein.
- the processing system 435 is a microfluidic qPCR system.
- the processing system 435 is an ALPHA screen or other plate reader.
- the processing system 435 is a FACS sorter or analyzer.
- the processing system 435 can be in communication with the system 401 through the network 430 , or by direct (e.g., wired, wireless) connection.
- raw data from the processing system e.g. a biomarker expression profile
- This data transfer may be direct (e.g. FTP, TCP, or other direct network connection between the processing system 435 and the system 401 ), or indirect (e.g. transfer to a cloud storage system which can be accessed by the system 401 ).
- Methods as described herein can be implemented by way of machine (or computer processor) executable code (or software) stored on an electronic storage location of the system 401 , such as, for example, on the memory 410 or electronic storage unit 415 .
- the code can be executed by the processor 405 .
- the code can be retrieved from the storage unit 415 and stored on the memory 410 for ready access by the processor 405 .
- the electronic storage unit 415 can be precluded, and machine-executable instructions are stored on memory 410 .
- the digital processing device includes one or more hardware central processing units (CPU) that carry out the device's functions.
- the digital processing device further comprises an operating system configured to perform executable instructions.
- the digital processing device is optionally connected a computer network.
- the digital processing device is optionally connected to the Internet such that it accesses the World Wide Web.
- the digital processing device is optionally connected to a cloud computing infrastructure.
- the digital processing device is optionally connected to an intranet.
- the digital processing device is optionally connected to a data storage device.
- suitable digital processing devices include, by way of non-limiting examples, server computers, desktop computers, laptop computers, notebook computers, sub-notebook computers, netbook computers, netpad computers, set-top computers, handheld computers, Internet appliances, mobile smartphones, tablet computers, personal digital assistants, video game consoles, and vehicles.
- server computers desktop computers, laptop computers, notebook computers, sub-notebook computers, netbook computers, netpad computers, set-top computers, handheld computers, Internet appliances, mobile smartphones, tablet computers, personal digital assistants, video game consoles, and vehicles.
- smartphones are suitable for use in the system described herein.
- Suitable tablet computers include those with booklet, slate, and convertible configurations, known to those of skill in the art.
- the digital processing device will normally include an operating system configured to perform executable instructions.
- the operating system is, for example, software, including programs and data, which manages the device's hardware and provides services for execution of applications.
- suitable server operating systems include, by way of non-limiting examples, FreeBSD, OpenBSD, NetBSD®, Linux, Apple® Mac OS X Server®, Oracle® Solaris®, Windows Server®, and Novell® NetWare®.
- suitable personal computer operating systems include, by way of non-limiting examples, Microsoft® Windows®, Apple® Mac OS X®, UNIX®, and UNIX-like operating systems such as GNU/Linux®.
- the operating system is provided by cloud computing.
- suitable mobile smart phone operating systems include, by way of non-limiting examples, Nokia® Symbian® OS, Apple® iOS®, Research In Motion® BlackBerry OS®, Google® Android®, Microsoft® Windows Phone® OS, Microsoft® Windows Mobile® OS, Linux®, and Palm® WebOS®.
- the device generally includes a storage and/or memory device.
- the storage and/or memory device is one or more physical apparatuses used to store data or programs on a temporary or permanent basis.
- the device is volatile memory and requires power to maintain stored information.
- the device is non-volatile memory and retains stored information when the digital processing device is not powered.
- the non-volatile memory comprises flash memory.
- the non-volatile memory comprises dynamic random-access memory (DRAM).
- the non-volatile memory comprises ferroelectric random-access memory (FRAM).
- the non-volatile memory comprises phase-change random access memory (PRAM).
- the device is a storage device including, by way of non-limiting examples, CD-ROMs, DVDs, flash memory devices, magnetic disk drives, magnetic tapes drives, optical disk drives, and cloud computing-based storage.
- the storage and/or memory device is a combination of devices such as those disclosed herein.
- a display to send visual information to a user will normally be initialized.
- Examples of displays include a cathode ray tube (CRT, a liquid crystal display (LCD), a thin film transistor liquid crystal display (TFT-LCD, an organic light emitting diode (OLED) display.
- CTR cathode ray tube
- LCD liquid crystal display
- TFT-LCD thin film transistor liquid crystal display
- OLED organic light emitting diode
- on OLED display is a passive-matrix OLED (PMOLED) or active-matrix OLED (AMOLED) display.
- the display can be a plasma display, a video projector or a combination of devices such as those disclosed herein.
- the digital processing device would normally include an input device to receive information from a user.
- the input device can be, for example, a keyboard, a pointing device including, by way of non-limiting examples, a mouse, trackball, track pad, joystick, game controller, or stylus; a touch screen, or a multi-touch screen, a microphone to capture voice or other sound input, a video camera to capture motion or visual input or a combination of devices such as those disclosed herein.
- the methods, kits, and systems disclosed herein can include one or more non-transitory computer readable storage media encoded with a program including instructions executable by the operating system to perform and analyze the test described herein; preferably connected to a networked digital processing device.
- the computer readable storage medium is a tangible component of a digital device that is optionally removable from the digital processing device.
- the computer readable storage medium includes, by way of non-limiting examples, CD-ROMs, DVDs, flash memory devices, solid state memory, magnetic disk drives, magnetic tape drives, optical disk drives, cloud computing systems and services, and the like.
- the program and instructions are permanently, substantially permanently, semi-permanently, or non-transitorily encoded on the media.
- a non-transitory computer-readable storage media can be encoded with a computer program including instructions executable by a processor to create or use a classification system.
- the storage media can comprise (a) a database, in a computer memory, of one or more clinical features of two or more control samples, wherein (i) the two or more control samples can be from two or more subjects; and (ii) the two or more control samples can be differentially classified based on a classification system comprising two or more classes; (b) a first software module configured to compare the one or more clinical features of the two or more control samples; and (c) a second software module configured to produce a classifier set based on the comparison of the one or more clinical features.
- At least two of the classes can be selected from preeclampsia, non-preeclampsia, normal pregnancy, complicated pregnancy, and gestational hypertension.
- Antigen/Biomarker Detection (E.g., Antibodies)
- the antigen binding reagent is used to detect any of the biomarkers identified herein.
- the antigen binding reagent can be an antibody (monoclonal or polyclonal), antigen-binding fragment (e.g. Fab, Fab′, F(ab) 2 , F(abc) 2 , or Fv fragment) of an antibody, or an antibody derivative (e.g. diabody, linear antibody, or scFv).
- the present disclosure provides for a method of determining whether a pregnant human female is at risk of suffering from preeclampsia comprising: determining a free Placental Growth Factor to dissociated Placental Growth Factor (PlGF-f:PlGF-d) ratio of a biological sample collected from the pregnant human female.
- the pregnant human female is identified as having a heightened risk of suffering from preeclampsia when a PlGF-f:PlGF-d ratio less than a first threshold value is determined.
- the pregnant human female is identified as having a reduced risk of suffering from preeclampsia when a PlGF-f:PlGF-d ratio greater than a second threshold value is determined.
- a PlGF-f:PlGF-d ratio less than a first threshold value indicates a heightened risk that the pregnant human female will suffer from preeclampsia.
- a PlGF-f:PlGF-d ratio greater than a second threshold value indicates a reduced risk that the pregnant human female will suffer from preeclampsia.
- the first and/or second threshold value can be determined by supervised learning using a trained algorithm.
- the trained algorithm can be trained using e.g. supervised learning approaches described herein.
- the method comprises collecting the biological sample.
- the biological sample may be collected by any of the methods described herein.
- the biological sample may be collected by venipuncture, fingerstick sampling, arterial sampling, or cervicovaginal fluid collection using an ophthalmic sponge.
- the biological sample may be any of the samples described for analysis herein.
- the biological sample may be a fluid, whole blood, peripheral blood, serum, plasma, urine, cervicovaginal secretion, or amniotic fluid sample.
- the treatment is an elevated temperature; e.g. least 30° C., at least 37° C., or at least 45° C.
- the treatment comprises increasing or decreasing the pH (e.g. to less than 6 or greater than 8).
- the treatment is a surfactant, such as an anionic surfactant, a cationic surfactant, a zwitterionic surfactant, a nonionic surfactant, SDS, PFOA, cetrimonium bromide (CTAB), cetylpyridinium chloride (CPC), benzalkonium chloride (BAC), benzethonium chloride (BZT), dimethyldioctadecylammonium chloride, dioctadecyldimethylammonium bromide (DODAB), CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate), cocamidopropyl hydroxysultaine, Triton-X-100, tween-20, tween-40-tween-60, or tween-80, or any combination thereof.
- a surfactant such as an anionic surfactant, a cationic surfactant, a
- the treatment is a chaotropic agent, such as a low concentration of urea or guanidinium hydrochloride.
- the treatment is an agent that adjusts the ionic strength of the solution, such as NaCl, KCl, or ammonium chloride.
- the treatment is a steric treatment.
- Steric treatments include antibodies, antibody fragments or derivatives, aptamers, peptides, proteins, or other agents that bind an epitope of free PlGF or free sFLT, PlGF, or sFLT1 and prevent reassembly of a PlGF/sFLT1complex.
- a PlGF binding or blocking agent is selected based on reference to a published crystal structure of PlGF protein (see e.g. Iyer et al. J Biol Chem. 2001 Apr. 13; 276(15):12153-61. Epub 2000 Nov. 7).
- the PlGF binding or blocking agent binds to residues on the PlGF-1 molecule that are involved in recognition of s-Flt-1.
- an agent, or an apparatus for dissociating PlGF are used in methods, systems, kits, or compositions described herein.
- agent or apparatus can include a device for application for heat to a sample from a subject (e.g. a multiwell plate in combination with a thermocycler or heat block), a pH adjustment agent (e.g.
- an ionic strength adjustment agent e.g., a concentrated solution of NaCl, KCl, or ammonium chloride
- a steric agent e.g., a concentrated solution of NaCl, KCl, or ammonium chloride
- the agent is provided in the form of a concentrated solution.
- the concentrated solution consists essentially of the reagent.
- the concentrated solution has a concentration of the agent of at least 50 mM, at least 100 mM, at least 150 mM, at least 200 mM, at least 250 mM, at least 300 mM, at least 350 mM, at least 400 mM, at least 450 mM, at least 500 mM, at least 550 mM, at least 600 mM, at least 650 mM, at least 700 mM, at least 750 mM, at least 800 mM, at least 850 mM, at least 900 mM, at least 950 mM, at least 1 M, at least 2 M, at least 4M, or at least 5 M.
- dissociated PlGF level or total PlGF level is measured by solid-phase extraction rather than dissociation per se.
- recombinant sFLT1 or another PlGF binding partner is added to the sample at a supersaturating concentration to bind all free PlGF, and the PlGF complex is captured using an sFLT antibody (or an antibody against the other PlGF binding partner).
- the captured PlGF is then dissociated from the sFLT (or the other PlGF binding partner) and measured using a PlGF ELISA, immunoassay, or mass spectrometry using standard temperature conditions.
- PlGF(f) is measured by anion exchange chromatography, cation exchange chromatography, or gel filtration chromatography or filtration by identifying a free PlGF peak separate from the other PlGF complexes.
- the PlGF complex following treatment to dissociate a PlGF complex, the PlGF complex is then treated with an agent to prevent reassembly of the PlGF complex with other agents.
- PlGF-d is not determined by dissociation but rather by binding of an antibody, antibody fragment, antibody derivative, aptamer, peptide binder, or protein binder with an alternative epitope to those described herein. In some cases, such an antibody binds PlGF at an epitope that is not blocked by binding of sFLT.
- Treatments to dissociate a PlGF complex may be applied for various amounts of time. In some embodiments, the treatment is applied for at least 5, 10, 15, 20, 25, 30, 40, 50, 60, 90, or 120 minutes, or at least 8, 12, or 16 hours.
- the first unmodified portion of said biological sample has not been stored at a temperature above about 20° C.
- the first unmodified sample is then contacted to a PlGF capture antibody at a temperature of e.g. 30° C., 28° C., 25° C., or 22° C. or less to prevent dissociation any PlGF:sFLT complexes in the sample.
- the first unmodified sample is contacted to a PlGF capture antibody with high affinity for a brief period of time to prevent dissociation of any PlGF:sFLT complexes in the sample.
- the PlGF is measured in a homogenous assay format (e.g. a format that does not require washing such as TR-FRET, LOCI, or alpha screen) to prevent dissociation of PlGF complexes.
- PlGF-d is measured at multiple time points in the same subject to determine a change in dissociation rate.
- PlGF-d may be measured every 2, 4, 6, 8, 12, 18, 24, 36, 48, 96, 120, 144, 168 or more hours.
- PlGF-f is not measured by preventing dissociation of PlGF complexes but rather by use of an antibody with an alternative epitope.
- an epitope may be an epitope specifically blocked by sFLT (e.g. a mono-mono antibody).
- PlGF-f is measured by preventing dissociation of other PlGF complexes using addition of a chemical crosslinking agent.
- a chemical crosslinking agent Such agents react with labile groups naturally present on protein surfaces (e.g. amino-, sulfhydryl-groups) to covalently stabilize protein-protein interactions.
- the crosslinking agent comprises an amine-to-amine crosslinking moiety such as an NHS ester (e.g. DSG, DSS, BS3, TSAT, BS(PEG)5, PS(PEG)9, DSP, DTSSP, DST, BSOCOES, EGS, Sulfo-EGS), an imidoester (e.g.
- the crosslinking agent comprises a sulfhydryl-to-sulfhydryl group crosslinker, such as an NHS-Haloacetyl Crosslinker (e.g. SIA, SBAP, SIAB, Sulfo-SIAB), NHS-maleimide (e.g.
- SPDP LC-SPDP, sulfo-LC-SPDP, SMPT, PEG4-SPDP, PEG12-SPDP
- the measurement of PlGF-f has a LLOQ of less than or equal to 10 pg/ml, 20 pg/ml, 30 pg/ml, 40 pg/ml, 50 pg/ml, or 100 pg/ml.
- the measurement of PlGF-d has a LLOQ of less than or equal to 10 pg/ml, 20 pg/ml, 30 pg/ml, 40 pg/ml, 50 pg/ml, or 100 pg/ml.
- the present disclosure provides for a method for determining a PlGF-f:PlGF-d ratio in a biological sample comprising PlGF, the method comprising: determining an amount of PlGF-f in a first aliquot of the biological sample by: contacting a first aliquot with a capture reagent for a first time at a first temperature, wherein the PIGF-f binds to the capture reagent to form a PIGF-f-capture reagent complex; contacting the PIGF-f-capture reagent complex with a detector reagent for a second time at a second temperature, wherein the PIGF-f-capture reagent complex binds to the second reagent to form a first ternary complex comprising PIGF-f, the capture reagent, and the detector reagent; and detecting the first ternary complex; and determining an amount of PlGF-d in a second aliquot of the biological sample by: contacting a second aliquot with the
- the first temperature is less than 30° C., less than 25° C., between 15° C. and 30° C., between 15° C. and 25° C., between 20° C. and 25° C., or about 22° C.
- the second temperature is less than 30 C, less than 25 C, between 15 C and 30 C, between 15° C. and 25° C., between 20° C. and 25° C., or about 22° C.
- the third temperature is at least 30° C., at least 35° C., between 30° C. and 45° C., about 30° C., about 37° C. or about 45° C.
- the fourth temperature is at least 30° C., at least 35° C., between 30° C. and 45° C., about 30° C., about 37° C. or about 45° C.
- the first time is less than 1 hour, less than 45 minutes, between 10 minutes and 30 minutes, about 30 minutes, about 20 minutes, or about 15 minutes.
- the second time is less than 1 hour, less than 45 minutes, between 10 minutes and 30 minutes, about 30 minutes, about 20 minutes, or about 15 minutes.
- the third time is at least 1 hour, between 1 hour and 3 hours, between 90 minutes and 150 minutes, or about 120 minutes.
- the fourth time is at least 1 hour, between 1 hour and 3 hours, between 90 minutes and 150 minutes, or about 120 minutes.
- the first time is about 30 minutes
- the second time is about 30 minutes
- the third time is about 2 hours
- the fourth time is about 2 hours
- the first temperature is about 22° C.
- the second temperature is about 22° C.
- the third temperature is about 37° C.
- the fourth temperature is about 37° C.
- the second step is conducted in the presence of a blocking antibody that prevents s-FLT from rebinding to PlGF.
- the disclosure provides assay kits for analysis of any of the sets of biomarkers included herein for the detection of preeclampsia.
- the assay kits comprise one or more antigen-binding reagents.
- the one or more antigen-binding reagents comprise combinations of antigen-binding reagents with specificities for the antigens/biomarkers presented below.
- the assay kits comprise one or more antigen-binding reagents in combination with a reagent capable of dissociating a PlGF complex, such as a pH adjustment agent (e.g.
- a surfactant e.g., an anionic surfactant, a cationic surfactant, a zwitterionic surfactant, a nonionic surfactant, SDS, PFOA, cetrimonium bromide (CTAB), cetylpyridinium chloride (CPC), benzalkonium chloride (BAC), benzethonium chloride (BZT), dimethyldioctadecylammonium chloride, dioctadecyldimethylammonium bromide (DODAB), CHAPS (3[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate), cocamidopropyl hydroxysultaine, Triton-X-100, tween-20, tween-40- tween-60, or tween
- CTAB cetrimonium bromide
- CPC cetylpyridinium chloride
- BAC benzalkonium chloride
- the assay kits comprise one or more antigen-binding reagents described herein in combination with: (a) an antibody, antibody fragment or derivative, aptamer, peptide, protein, or other agent that binds an epitope that reports total (e.g.
- the reagent capable of dissociating a PlGF complex is provided in the form of a concentrated solution.
- the concentrated solution consists essentially of the reagent.
- the concentrated solution has a concentration of the reagent of at least 50 mM, at least 100 mM, at least 150 mM, at least 200 mM, at least 250 mM, at least 300 mM, at least 350 mM, at least 400 mM, at least 450 mM, at least 500 mM, at least 550 mM, at least 600 mM, at least 650 mM, at least 700 mM, at least 750 mM, at least 800 mM, at least 850 mM, at least 900 mM, at least 950 mM, at least 1 M, at least 2 M, at least 4M, or at least 5 M.
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for one or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1 or Endoglin; and (b) PlGF (free, dissociated, or % free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1 or Endoglin; (b) PlGF (free, dissociated, or % free); and (c) KIM1.
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1 or Endoglin; (b) PlGF (free, dissociated, or % free); (c) KIM1; and (d) FGF21.
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1 or Endoglin; (b) PlGF (free, dissociated, or % free); (c) KIM1; and (d) Decorin.
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1 or Endoglin; (b) PlGF (free, dissociated, or % free); (c) KIM1; (d) FGF21; and (e) Decorin.
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1; (b) Endoglin; and (c) PlGF (free, dissociated, or % free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1; (b) Endoglin; (c) PlGF (free, dissociated, or % free); and (d) KIM1.
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1; (b) Endoglin; (c) PlGF (free, dissociated, or % free); (d) KIM1; and (e) FGF21.
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1; (b) Endoglin; (c) PlGF (free, dissociated, or % free); (d) KIM1; and (e) Decorin.
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1; (b) Endoglin; (c) PlGF (free, dissociated, or % free); (d) KIM1; (e) FGF21; and (f) Decorin.
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for two or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for three or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for four or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for five or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for six or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for seven or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for all of sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for two biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for three biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for four biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for five biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for six biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for seven biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for no more than two biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for no more than three biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for no more than four biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for no more than five biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for no more than six biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for no more than seven biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- the assay kit provided is suitable for a multiplex homogenous biomarker assay, suitable for detection of all the analytes in a single reaction (e.g. in the same solution compartment).
- multiple antibodies or antigen detection reagents that bind to separate epitopes are provided against the same analyte/biomarker, and detection of coincident binding/interaction of both antibodies to the same molecule of analyte/biomarker serves to detect the analyte/biomarker in the sample.
- kits provide two antibodies or antigen-binding reagents against each analyte.
- kits provide a pair of antibodies or antigen-binding reagents for each analyte conjugated to a complementary pair of FRET dyes, wherein one antibody or antigen-binding reagent of the pair is conjugated to a FRET donor and the other is conjugated to a FRET acceptor.
- kits provide a pair of antibodies or antigen-binding reagents wherein one antibody or antigen-binding reagent of the pair is conjugated to a photosensitizer and the other antibody or antigen-binding reagent of the pair is conjugated to an oxygen sensitive dye.
- the assay kit provided is suitable for a multiplex non-homogenous biomarker assay suitable for detection of all the analytes in separate reactions (e.g. in separate solution compartments).
- assays e.g. sandwich ELISA
- antibodies or antigen binding reagents against the relevant set of biomarkers are provided attached to a substrate (e.g. in a well of a multiwell plate, or in a lateral flow assay lane).
- a second free antibody against each of the biomarkers provided attached to the substrate is also provided; this antibody can be labeled (e.g. with a fluorescent dye, with a chemiluminescent enzyme, or a luminescent enzyme) or unlabeled.
- a secondary labeled (e.g. with a fluorescent dye, with a chemiluminescent enzyme, or a luminescent enzyme) antibody or antigen-binding reagent is provided which has binding specificity against the second free antibody.
- the kit is for use as an in vitro diagnostic kit that includes one or more cartridges with reagents for testing on third-party platforms. Data collected from third-parties could be uploaded to a server (the cloud), put through a model/algorithm, and results can be shared with a doctor or other medical practitioner. Kits may also include instructions for use (e.g., according to any of the methods described herein).
- the assay kits comprise apparatus for collecting a biological sample from a pregnant female subject.
- apparatus can include, but is not limited to, an ophthalmic sponge or collection tube, with or without any reagents or preservatives necessary for sample preparation or stable storage (e.g. any of anti-microbials such as sodium azide and sodium fluoride, anti-coagulants such as EDTA, sodium citrate, potassium oxalate, heparin, and acid citrate dextrose, or fixatives such as methanol, ethanol, and formaldehyde).
- Collection tubes may be configured for or suitable for collection of any of the samples described herein, such as blood, peripheral blood, serum, plasma, urine, cervicovaginal mucus, or saliva. Collection tubes include e.g., red-top tubes; mottled red/gray-top, gold-top, or cherry red-top (gel-barrier) tubes; lavender-top tubes, and gray-top tubes.
- the assay kits comprise apparatus facilitating any of the assays described herein.
- apparatus can include, but is not limited to, lateral flow strips or multiwell plates containing any of the antibodies or antigen-binding fragments thereof described herein conjugated or adsorbed to a surface thereof.
- the assay kits comprise instructions for carrying out any of the assays described herein.
- assays include immunoassays, ELISA assays, sandwich ELISA assays, TR-FRET assays, proximity extension assays, amplified luminescent proximity (LOCI) assays, or luminescent oxygen channeling immunoassays.
- LOCI amplified luminescent proximity
- the assay kits comprise no more than 20, no more than 15, no more than 10, no more than 9, no more than 8, no more than 7, no more than 6, nor more than 5, no more than 4, no more than 3, no more than 2, or no more than 1 probes, antibodies, or antigen binding fragments or derivatives of antibodies specific for any of the biomarkers described herein.
- experiments were first performed on third-trimester pregnant patient serum samples naturally containing various quantities of sFLT (e.g. 1-64 ng/ml sFLT and applying various incubation conditions (2 hours capture at 37° C. or 45° C., while keeping incubation with detection antibody after wash at 22° C.) to the serum samples to optimize capture incubation time (see FIG. 3 A ).
- sFLT e.g. 1-64 ng/ml sFLT
- incubation time for capture was optimized.
- a separate set of third-trimester serum samples also containing variable sFLT concentrations as above (e.g. 1-64 ng/ml sFLT) to capture incubations at 37° C. for various different incubation times, varying capture and post-wash detection incubation times simultaneously (see FIG. 4 A , where the x-axis shows the incubation time used for both the PlGF capture and detection incubation).
- PlGF-f and PlGF-d quantification were established based on the results of temperature and time optimization.
- PlGF-f is measured at less than 22° C. with a 30-minute capture incubation a 30-minute detector incubation; and PlGF-d is measured at 37° C. with a 2-hour capture incubation and a 2-hour detector incubation.
- the legacy PlGF ELISA protocol PlGF(II)
- FIG. 6 shows plots of PE and non-PE patients from the studies segregated using PlGF(f) conc or % free PlGF ((f)/(d) ⁇ 100) demonstrating that both show improved separation of PE samples from non-PE samples.
- FIG. 7 A shows the ⁇ f and ⁇ d levels measured for preeclamptic and non-preeclamptic patients alongside results from the legacy PlGF assay (“PlGF(II)”, which was determined above to measure intermediate concentrations of dissociated PlGF).
- PlGF(f) provided better discrimination between PE and non-PE samples than PlGF(II) or PlGF(d) individually.
- Graphing the same data as free PlGF versus sFLT ( FIG. 7 B ) and % free PlGF vs sFLT ( FIG. 7 C ) demonstrated that use of a PlGF f:d ratio (such as % free PlGF) increased the dynamic range of PlGF measurement between non-PE and PE samples and improved their separation (see blue vs orange dots in FIGS. 7 B and 7 C ).
- Blood samples are collected from pregnant patients using red top blood collection tubes; the tubes are allowed to clot at room temperature for 30-60 minutes, are centrifuged 20 minutes at 1300 g to remove the clot and are aliquoted for long term storage at below ⁇ 80° C.
- Blood samples are collected from pregnant patients using red top blood collection tubes; the tubes are allowed to clot at room temperature for 30-60 minutes, are centrifuged 20 minutes at 1300 g to remove the clot and are aliquoted for long term storage below ⁇ 80° C.
- R&D Systems Quantikine kit (Catalog number DPG00) is used to measure the free form of PlGF [PlGF(f)].
- 100 ul of assay diluent RD1-22 (part #895490) is added to each well of the PlGF microplate coated with the monoclonal anti-PlGF antibody (part #890509).
- Each sample is diluted 1:2 in calibrator diluent RD6-11 (part #895489) and 100 ul of a diluted serum sample is aliquoted into each well of the 96-well plate.
- the serum samples are incubated in the plate with low shaking for 30 minutes at ambient temperature. Following incubation, the sample from each well of the plate was aspirated and the well was not washed.
- R&D Systems Quantikine kit (Catalog number DPG00) is used to measure the dissociated form of PlGF (PlGF(d)].
- PlGF PlGF(d)
- 100 ul of assay diluent RD1-22 (part #895490) is added to each well of the PlGF microplate coated with the monoclonal anti-PlGF antibody (part #890509).
- Each sample is diluted 1:2 in calibrator diluent RD6-11 (part #895489) and 100 ul of a diluted serum sample is aliquoted into each well of the 96-well plate.
- the serum samples are incubated in the plate with low shaking for 120 minutes at 37° C.
- PRO-105 Pregnant women who were 18 years or older (20 weeks to 39 weeks of gestation at first visit) with suspected preeclampsia (based on new onset symptoms, elevated blood pressure, proteinuria, edema or others) were selected for participation in the PRO-105 study. Baseline procedures were performed, including collection of demographic, medical and obstetric histories, list of concomitant medications, weight, height, blood pressure, and other clinical information, as well as obtaining blood and urine samples for use in biomarker assays. After discharge, all patients in the study were followed by interim research visits every 14 days (+/ ⁇ 3 days). For patients who developed PreE, the time (in days) from baseline sampling, the gestational age at diagnosis, and the severity of the disease was recorded.
- NEGATIVE-PRE-E CONTROL Patients who did not develop preeclampsia before or at delivery were included in the NEGATIVE-PRE-E CONTROL (NonPreE) group.
- NEGATIVE PRE-E CONTROLS the time from baseline sampling (in days) to either delivery or loss to follow-up was recorded.
- Delivery outcomes were collected on all subjects enrolled in the study. Additionally, if possible, during admission for delivery, blood and urine samples were collected for analysis at delivery.
- PRO-104 Select samples from PRO-104 were used as positive controls to further develop the assay. Pregnant women who were 18 to 45 years of age with 32 weeks 0 days to 36 weeks and 6 days weeks of gestation age who presented at clinical sites with signs and symptoms of preeclampsia (based on new onset or worsening of symptoms including elevated blood pressure, proteinuria, edema amongst others) were eligible for participation in the PRO-104 study. Baseline procedures were performed, including collection of demographic, medical and obstetric histories, list of concomitant medications, weight, height, blood pressure, and other clinical information, as well as obtaining blood and urine samples for use in biomarker assays. Study subjects were followed by several study visits until the delivery visit.
- Subjects who did not develop preeclampsia before or at delivery or subjects who were diagnosed with PreE after 24 h of enrollment were included in the NEGATIVE-PRE-E CONTROL (NonPreE) cohort. For all subjects, the time from baseline sampling (in days) to either delivery or loss to follow-up was recorded.
- Delivery outcomes were collected on all subjects enrolled in the study. Additionally, if possible, during admission for delivery, blood and urine samples were collected for analysis at delivery.
- Example 4 Use of VEGF as an Additional Diagnostic Marker for Preeclampsia
- PlGF-f, PlGF-d, sFlt are measured in serum samples from pregnant women.
- VEGF is additionally measured in the serum from pregnant women with high PIGF-d, and/or high sFlt.
- VEGF above 10 pgs/ml or 50 pgs/ml or 100 pgs/ml is indicative of a reduced risk of preeclampsia.
- Example 5 Establishing Threshold for Test Using PlGF-d and PlGF-f in Detection of Preeclampsia or Absence Thereof
- Threshold analysis was performed to identify a threshold for a univariate model utilizing % PlGF free (PlGF free /PlGF dissociated ) as a predictor of preeclampsia or absence thereof with a single cutoff using the PlGF free /PlGF dissociated protocols described in Example 2 (in this model, % PlGF free levels higher than the threshold indicate preeclampsia).
- An AUC curve analysis of % PlGF free in the PRO-105 study populations from Example 3 were performed (see FIG. 12 ). 100 repeats of 10-fold cross-validation on the data from the study population was used to identify a threshold that achieved 90% sensitivity on the study population; the threshold was set at the 25 th percentile of the 100 repeats of 10-fold cross validation. The 25 th percentile threshold (Q25 Threshold) was determined to be ⁇ 0.046; this threshold alongside other threshold distribution data is summarized below and in FIG. 13 :
- the target population for the study was pregnant women 18 to 45 years of age and 28 to 36 6/7 weeks gestational age who presented with signs and symptoms of PE, including one or more of: (i) new onset increased blood pressure in an otherwise normotensive patient; (ii) worsening hypertension in a patient with pre-existing hypertension; (iii) new onset proteinuria or worsening of pre-existing proteinuria; (iv) any other clinical finding typically associated with suspicion of preeclampsia and requiring workup to rule-out preeclampsia.
- the study demographics are presented below in Table B.
- the samples from the PRO-129 study were re-segregated into either “cases” or “non-cases” as shown in FIG. 15 B .
- the clinical status of a subject is considered “case” if the preeclampsia diagnosis happens within the rule-out window and subject delivers before 37 0/7 weeks of gestation, and the clinical status of a subject is considered as ‘non-case’ if no diagnosis of Preeclampsia happens during the pregnancy or diagnosis of preeclampsia happens outside of the rule-out window or the diagnosis of preeclampsia happens within the rule-out-window but the subject delivers after 36 6/7 weeks of gestation
Abstract
Description
- This application is a continuation of U.S. application Ser. No. 17/393,708, filed Aug. 4, 2021, which is a continuation of U.S. application Ser. No. 17/119,410, filed Dec. 11, 2020, now U.S. Pat. No. 11,112,403, which is a continuation of International Application No. PCT/US2020/063408, filed Dec. 4, 2020, which claims the benefit of U.S. Provisional Application No. 62/943,739 entitled “Detection Of Preeclampsia Using Assays For Free And Dissociated Placental Growth Factor”, filed on Dec. 4, 2019 and U.S. Provisional Application No. 62/947,957 entitled “Detection Of Preeclampsia Using Assays For Free And Dissociated Placental Growth Factor”, filed on Dec. 13, 2019, all of which are incorporated by reference herein in their entireties.
- Preeclampsia is a serious multisystem complication of pregnancy. The incidence of the disorder is generally considered to be between 2% to 8% of all pregnancies, and the disorder carries significant morbidity and mortality risks for both mothers and infants. Preeclampsia is the second largest cause of maternal/fetal deaths and is responsible for approximately twenty billion dollars in healthcare costs annually. In the United States, approximately one million women present with classical symptoms of preeclampsia (hypertension and/or proteinuria after the 20th month of gestation) each year.
- The cause(s) and pathogenesis of preeclampsia remain uncertain, and the identification (or ruling out) of preeclampsia using the classical clinical symptoms of the disease is non-ideal. The presentation of classical clinical symptoms can be highly variable, and the symptoms can be indicative of other distinct disorders, such as chronic hypertension, gestational hypertension, temporary high blood pressure, and gestational diabetes. Current laboratories tests (e.g., tests for proteinuria) can be prone to inaccuracies, or are useful for detection of preeclampsia only during relatively late periods in the progression of the disorder. Methods for more reliably predicting whether a pregnant woman will or will not have preeclampsia may, among other things, (1) lead to more timely diagnosis, (2) improve the accuracy of a diagnosis, and/or (3) prevent the unnecessary treatment of women with some preeclampsia symptoms.
- Previous studies have used free levels of the vascular factors PlGF and/or sFLT as biomarkers of preeclampsia. However, levels of these free factors alone measured using known methods have limited sensitivity and specificity.
- In some aspects, the present disclosure provides for a method of determining whether a pregnant human female is at risk of suffering from preeclampsia comprising: determining a free Placental Growth Factor to dissociated Placental Growth Factor (PlGF-f:PlGF-d) ratio of a biological sample collected from the pregnant human female, wherein a PlGF-f:PlGF-d ratio less than a first threshold value indicates a heightened risk that the pregnant human female will suffer from preeclampsia, and wherein a PlGF-f:PlGF-d ratio greater than a second threshold value indicates a reduced risk that the pregnant human female will suffer from preeclampsia. In some embodiments, the method comprises collecting the biological sample. In some embodiments, the biological sample is a fluid, whole blood, peripheral blood, serum, plasma, urine, or amniotic fluid sample. In some embodiments, the method comprises collecting the biological sample via venipuncture, fingerstick sampling, or arterial sampling. In some embodiments, the biological sample is collected from the pregnant woman at
gestational week 20 or later. In some embodiments, the biological sample is collected from the pregnant woman prior togestational week 30. In some embodiments, the risk of developing preeclampsia is the risk of developing preeclampsia within the following one, two, three or more weeks. In some embodiments, the PlGF-f:PlGF-d ratio is determined at least in part by: (a) determining a PlGF-f level by contacting a first PlGF probe to a first portion of the biological sample; and (b) determining a PlGF-d level by contacting the first PlGF probe to a second portion of the biological sample, wherein the first portion of the biological sample is untreated, and wherein a treatment is applied to the second portion of the biological sample, and wherein the treatment dissociates a PlGF complex. In some embodiments, the PlGF complex comprises PlGF and s-FLT. In some embodiments, the treatment is applied for at least 5, 10, 15, 20, 25, 30, 40, 50, 60, 90, or 120 minutes. In some embodiments, the treatment comprises increasing the temperature of the second portion of the biological sample. In some embodiments, the treatment comprises increasing the temperature to at least 30° C., at least 37° C., or at least 45° C. for at least 1 hour or at least 2 hours. In some embodiments, the treatment comprises increasing or decreasing the pH of the second portion of the biological sample. In some embodiments, the treatment comprises contacting the second portion of the biological sample with an agent that prevents PlGF from assembling into a PlGF complex. In some embodiments, the agent is selected from: a surfactant, a detergent, a chaotropic agent, a PlGF-binding protein, and an s-FLT-binding protein. In some embodiments, the first unmodified portion of the biological sample has not been stored at a temperature above about 20° C. In some embodiments, the method has a LLOQ for PlGF of 20 pg/ml or less. In some embodiments, the method comprises (a) determining a level of at least one secondary biomarker selected from total sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274; and (b) determining the pregnant woman as being at risk or not at risk of preeclampsia by application of a trained algorithm to the PlGF-f:PlGF-d ratio in combination with the determined level least one of the secondary biomarker In some embodiments, the trained algorithm comprises a logistic regression, random forest, nearest centroid, gradient boosting method, linear discriminant analysis, neural network, or support vector machine algorithm. In some embodiments, the level of the at least one secondary biomarker is determined by an immunoassay. In some embodiments, the immunoassay is selected from a capture ELISA, an indirect ELISA, a TR-FRET assay, a proximity extension assay, an amplified luminescent proximity (LOCI) assay, a luminescent oxygen channeling immunoassay, or a lateral flow assay. - In some aspects, the present disclosure provides for a method of treating a pregnant human female comprising: determining whether the pregnant human female has an elevated risk of preeclampsia or reduced risk of preeclampsia by: obtaining a biological sample from the pregnant human female, determining a PlGF-f:PlGF-d ratio in the biological sample, and optionally determining a level of one or more secondary biomarkers in the biological sample, applying a classifier to the PlGF-f:PlGF-d ratio and, optionally, the level of one or more secondary biomarkers to classify the pregnant human female as having an elevated risk of preeclampsia or a reduced risk of preeclampsia; if the pregnant human female has an elevated risk of preeclampsia, then determining the blood pressure of the pregnant human female at a frequency of at least one time per week, and if the pregnant human female has a reduced risk of preeclampsia, then determining the blood pressure of the pregnant human female at a frequency of less than one time per week. In some embodiments, the method comprises, if the pregnant human female is determined to have an elevated risk of preeclampsia, then administering an antihypertensive drug to the pregnant human female. In some embodiments, the antihypertensive drug is a central alpha agonist, a vasodilator, a calcium-channel blocker, an alpha-blocker, acetylsalicylic acid, or a beta-blocker. In some embodiments, the antihypertensive drug is methyldopa, labetalol, nifedipine, verapamil, clonidine, hydralazine, diazoxide, prazosin, or oxprenolol. In some embodiments, the method comprises administering a cervical ripening or labor-inducing agent to the pregnant woman when the pregnant woman is determined to be at risk of suffering from preeclampsia. In some embodiments, the cervical ripening or labor-inducing agent comprises a prostaglandin, oxytocin, misoprostol, mifepristone, or relaxin.
- In some embodiments, the present disclosure provides for a method for avoiding unnecessary treatment of preeclampsia, the method comprising: (a) contacting a biological sample that has been collected from a pregnant human female with a plurality of different antigen-specific probes to determine levels of three or more markers, wherein the three or more markers comprise at least (i) PlGF-f and PlGF-d; and (ii) at least one of sFLT, Endoglin, KIM-1, FGF21, or free sFLT; and (b) proceeding with treatment of the pregnant human in a manner that avoids unnecessary treatment of preeclampsia based at least in part on the amounts or concentrations of the three or more markers determined in (a). In some embodiments, the method comprises collecting the biological sample. In some embodiments, the biological sample is a fluid, whole blood, peripheral blood, serum, plasma, urine, or amniotic fluid sample. In some embodiments, the method comprises collecting the biological sample via venipuncture, fingerstick sampling, or arterial sampling. In some embodiments, the biological sample is collected from the pregnant woman at
gestational week 20 or later. In some embodiments, the biological sample is collected from the pregnant woman prior togestational week 30. In some embodiments, the risk of developing preeclampsia is the risk of developing preeclampsia within the following one, two, three or more weeks. In some embodiments, the PlGF-f:PlGF-d ratio is determined at least in part by: (a) determining a PlGF-f level by contacting a first PlGF probe to a first portion of the biological sample; and (b) determining a PlGF-d level by contacting the first PlGF probe to a second portion of the biological sample, wherein the first portion of the biological sample is untreated, and wherein a treatment is applied to the second portion of the biological sample, and wherein the treatment dissociates a PlGF complex. In some embodiments, the PlGF complex comprises PlGF and s-FLT. In some embodiments, the treatment is applied for at least 5, 10, 15, 20, 25, 30, 40, 50, 60, 90, or 120 minutes. In some embodiments, the treatment comprises increasing the temperature of the second portion of the biological sample. In some embodiments, the treatment comprises increasing the temperature to at least 30° C., at least 37° C., or at least 45° C. for at least 1 hour or at least 2 hours. In some embodiments, the treatment comprises increasing or decreasing the pH of the second portion of the biological sample. In some embodiments, the treatment comprises contacting the second portion of the biological sample with an agent that prevents PlGF from assembling into a PlGF complex. In some embodiments, the agent is selected from: a surfactant, a detergent, a chaotropic agent, a PlGF-binding protein, and an s-FLT-binding protein. In some embodiments, the first unmodified portion of the biological sample has not been stored at a temperature above about 20° C. In some embodiments, the method has a LLOQ for PlGF of 20 pg/ml or less. In some embodiments, the method comprises (a) determining a level of at least one secondary biomarker selected from total sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274; and (b) determining the pregnant woman as being at risk or not at risk of preeclampsia by application of a trained algorithm to the PlGF-f:PlGF-d ratio in combination with the determined level least one of the secondary biomarker In some embodiments, the trained algorithm comprises a logistic regression, random forest, nearest centroid, gradient boosting method, linear discriminant analysis, neural network, or support vector machine algorithm. In some embodiments, the level of the at least one secondary biomarker is determined by an immunoassay. In some embodiments, the immunoassay is selected from a capture ELISA, an indirect ELISA, a TR-FRET assay, a proximity extension assay, an amplified luminescent proximity (LOCI) assay, a luminescent oxygen channeling immunoassay, or a lateral flow assay. - In some embodiments, the present disclosure provides for a system for ruling out preeclampsia in a pregnant female subject for a specified period of time, the system comprising: a processor; an input module for inputting levels of at least three markers in a biological sample, wherein the at least three markers comprise at least (i) PlGF-f and PlGF-d; and (ii) at least one of sFLT, Endoglin, KIM-1, FGF21, or free sFLT; a computer readable medium containing instructions that, when executed by the processor, perform a first algorithm on the input levels of the at least three markers; and an output module providing one or more indicia based on the input levels of the at least three markers, wherein the one or more indicia are indicative of the subject not having preeclampsia for at least a specified period of time. In some embodiments, the biological sample is a fluid, whole blood, peripheral blood, serum, plasma, urine, or amniotic fluid sample. In some embodiments, the biological sample is collected from the pregnant woman at
gestational week 20 or later. In some embodiments, the biological sample is collected from the pregnant woman prior togestational week 30. In some embodiments, the risk of developing preeclampsia is the risk of developing preeclampsia within the following one, two, three or more weeks. In some embodiments, the PlGF-f:PlGF-d ratio is determined at least in part by: (a) determining a PlGF-f level by contacting a first PlGF probe to a first portion of the biological sample; and (b) determining a PlGF-d level by contacting the first PlGF probe to a second portion of the biological sample, wherein the first portion of the biological sample is untreated, and wherein a treatment is applied to the second portion of the biological sample, and wherein the treatment dissociates a PlGF complex. In some embodiments, the PlGF complex comprises PlGF and s-FLT. In some embodiments, the treatment is applied for at least 5, 10, 15, 20, 25, 30, 40, 50, 60, 90, or 120 minutes. In some embodiments, the treatment comprises increasing the temperature of the second portion of the biological sample. In some embodiments, the treatment comprises increasing the temperature to at least 30° C., at least 37° C., or at least 45° C. for at least 1 hour or at least 2 hours. In some embodiments, the treatment comprises increasing or decreasing the pH of the second portion of the biological sample. In some embodiments, the treatment comprises contacting the second portion of the biological sample with an agent that prevents PlGF from assembling into a PlGF complex. In some embodiments, the agent is selected from: a surfactant, a detergent, a chaotropic agent, a PlGF-binding protein, and an s-FLT-binding protein. In some embodiments, the first unmodified portion of the biological sample has not been stored at a temperature above about 20° C. In some embodiments, the method has a LLOQ for PlGF of 20 pg/ml or less. In some embodiments, the method comprises (a) determining a level of at least one secondary biomarker selected from total sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274; and (b) determining the pregnant woman as being at risk or not at risk of preeclampsia by application of a trained algorithm to the PlGF-f:PlGF-d ratio in combination with the determined level least one of the secondary biomarker In some embodiments, the trained algorithm comprises a logistic regression, random forest, nearest centroid, gradient boosting method, linear discriminant analysis, neural network, or support vector machine algorithm. In some embodiments, the level of the at least one secondary biomarker is determined by an immunoassay. In some embodiments, the immunoassay is selected from a capture ELISA, an indirect ELISA, a TR-FRET assay, a proximity extension assay, an amplified luminescent proximity (LOCI) assay, a luminescent oxygen channeling immunoassay, or a lateral flow assay. - In some aspects, the present disclosure provides for a method for determining a PlGF-f:PlGF-d ratio in a biological sample comprising: (a) isolating a first aliquot of the biological sample for the detection of PlGF-f and a second aliquot of the biological sample for the detection of PlGF-d; (b) determining an amount of PlGF-f in the first aliquot; (b) applying a treatment to the second aliquot to dissociate PlGF complexes; and (c) determining an amount of PlGF-d in the second aliquot. In some embodiments, the biological sample has not been stored at a temperature above about 20° C. In some embodiments, the amount of PlGF-f and the amount of PlGF-d are determined using the same reagents. In some embodiments, the PlGF complex comprises PlGF and s-FLT. In some embodiments, the treatment is applied for at least 5, 10, 15, 20, 25, 30, 40, 50, 60, 90, or 120 minutes. In some embodiments, the treatment comprises increasing the temperature of the second portion of the biological sample. In some embodiments, the treatment comprises increasing the temperature to at least 30° C. for at least 1 hour. In some embodiments, the treatment comprises increasing or decreasing the pH of the second portion of the biological sample. In some embodiments, the treatment comprises contacting the second portion of the biological sample with an agent that prevents PlGF from assembling into a PlGF complex. In some embodiments, the agent is selected from: a surfactant, a detergent, a chaotropic agent, a PlGF-binding protein, and an s-FLT-binding protein. In some embodiments, the method has a LLOQ for PlGF of 20 pg/ml or less.
- In some aspects, the present disclosure provides for a method for determining a PlGF-f:PlGF-d ratio in a biological sample comprising PlGF, the method comprising, (a) determining an amount of PlGF-f in a first aliquot of the biological sample by: (i) contacting the first aliquot with a capture reagent for a first time at a first temperature, wherein the PIGF-f binds to the capture reagent to form a PIGF-f-capture reagent complex; (ii) contacting the PIGF-f-capture reagent complex with a detector reagent for a second time at a second temperature, wherein the PIGF-f-capture reagent complex binds to the second reagent to form a first ternary complex comprising PIGF-f, the capture reagent, and the detector reagent; and (iii) detecting the first ternary complex; and (b) determining an amount of PlGF-d in a second aliquot of the biological sample by: (i) contacting the second aliquot with the capture reagent for a third time at a third temperature, wherein the PIGF-d binds to the capture reagent to form a PIGF-d-capture reagent complex; (ii) contacting the PIGF-d-capture reagent complex with the detector reagent for a fourth time at a fourth temperature, wherein the PIGF-d-capture reagent complex binds to the second reagent to form a second ternary complex comprising PIGF-d, the capture reagent, and the detector reagent; and (iii) detecting the second ternary complex; wherein a PlGF-s-Flt1 complex is stable at the first temperature and the PlGF-s-Flt1 complex dissociates at the second temperature. In some embodiments, (a) the first temperature is less than 30° C., less than 25° C., between 15° C. and 30° C., between 15° C. and 25° C., between 20° C. and 25° C., or about 22° C.; (b) the second temperature is less than 30 C, less than 25 C, between 15 C and 30 C, between 15° C. and 25° C., between 20° C. and 25° C., or about 22° C.; (c) the third temperature is at least 30° C., at least 35° C., between 30° C. and 45° C., about 30° C., about 37° C. or about 45° C.; (d) the fourth temperature is at least 30° C., at least 35° C., between 30° C. and 45° C., about 30° C., about 37° C. or about 45° C.; (e) the first time is less than 1 hour, less than 45 minutes, between 10 minutes and 30 minutes, about 30 minutes, about 20 minutes, or about 15 minutes; (f) the second time is less than 1 hour, less than 45 minutes, between 10 minutes and 30 minutes, about 30 minutes, about 20 minutes, or about 15 minutes; (g) the third time is at least 1 hour, between 1 hour and 3 hours, between 90 minutes and 150 minutes, or about 120 minutes; and (h) the fourth time is at least 1 hour, between 1 hour and 3 hours, between 90 minutes and 150 minutes, or about 120 minutes. In some embodiments, the first time is about 30 minutes, the second time is about 30 minutes, the third time is about 2 hours, the fourth time is about 2 hours, the first temperature is about 22° C., the second temperature is about 22° C., the third temperature is about 37° C., and the fourth temperature is about 37° C. In some embodiments, step b is conducted in the presence of a blocking antibody that prevents s-FLT from rebinding to PlGF. In some embodiments, the biological sample is blood, serum, plasma, amniotic fluid, or urine. In some embodiments, the biological sample is serum or plasma. In some embodiments, the biological sample is collected from a pregnant human female. In some embodiments, the pregnant human female has been pregnant for at least 20 weeks. In some embodiments, the pregnant human female has been pregnant for no more than 30 weeks. In some embodiments, the biological sample is stored at a storage temperature and the storage temperature never exceeds 30° C. In some embodiments, the storage temperature is less than 0° C. In some embodiments, the storage temperature is about −80° C. In some embodiments, the biological sample is diluted prior to step a. In some embodiments, the capture reagent comprises an antibody or an antigen-binding fragment thereof. In some embodiments, the detector reagent comprises an antibody or an antigen-binding fragment thereof. In some embodiments, the capture reagent is attached to a solid support. In some embodiments, the detector reagent is attached to a solid support. In some embodiments, the detector reagent comprises a detectable label. In some embodiments, the label is a fluorophore, an enzyme, or an electrochemiluminescent tag. In some embodiments, (a) further comprises contacting the first aliquot with a crosslinking agent that prevents the dissociation of a PlGF-sFLT complex.
- In some aspects, the present disclosure provides for a method for determining a PlGF-f:PlGF-d ratio in a biological sample, wherein PlGF-f is determined using a reagent that can bind to PlGF-f but cannot bind to PlGF-d. In some embodiments, the reagent is an antibody or antigen-binding fragment thereof. In some embodiments, the reagent binds to residues on the PlGF-1 molecule that are involved in recognition of s-Flt-1.
- In some aspects, the present disclosure provides for a method for determining a PlGF-f:PlGF-d ratio in a biological sample, wherein PlGF-d is determined using a reagent that can bind to PlGF-d but cannot bind to PlGF-f. In some embodiments, the reagent is an antibody or antigen-binding fragment thereof. In some embodiments, the reagent binds to the interface between PlGF and s-Flt-1.
- In some aspects, the present disclosure provides for a method for determining a PlGF-f:PlGF-d ratio in a biological sample comprising separating the biological sample into a free PlGF component and a PlGF complex component, determining the amount of PlGF in the free PlGF component, and determining the amount of PlGF in the PlGF complex component. In some embodiments, the separating is performed by anion exchange chromatography, cation exchange chromatography, gel filtration chromatography or filtration. In some embodiments, the biological sample is contacted with a chemical crosslinking agent prior to the separating.
- In some aspects, the present disclosure provides for a method of determining whether a pregnant human female is at risk of suffering from preeclampsia comprising: (a) determining a free Placental Growth Factor to dissociated Placental Growth Factor (PlGF-f:PlGF-d) ratio of a biological sample collected from the pregnant human female, wherein a PlGF-f:PlGF-d ratio less than a first threshold value indicates a heightened risk that the pregnant human female will suffer from preeclampsia, and wherein a PlGF-f:PlGF-d ratio greater than a second threshold value indicates a reduced risk that the pregnant human female will suffer from preeclampsia. In some embodiments, the first threshold value and the second threshold value are the same value. In some embodiments, the method comprises collecting the biological sample. In some embodiments, the biological sample is a fluid, whole blood, peripheral blood, serum, plasma, urine, or amniotic fluid sample. In some embodiments, the method further comprises collecting the biological sample via venipuncture, fingerstick sampling, or arterial sampling. In some embodiments, the biological sample is collected from the pregnant woman at
gestational week 20 or later. In some embodiments, the biological sample is collected from the pregnant woman atgestational week 28 or later. In some embodiments, the biological sample is collected from the pregnant woman atgestational week 35 or later. In some embodiments, the biological sample is collected from the pregnant woman prior togestational week 30. In some embodiments, the biological sample is collected from the pregnant woman prior togestational week 37. In some embodiments, the biological sample is collected from the pregnant woman atgestational week 28 to 36 6/7. In some embodiments, the biological sample is collected from the pregnant woman atgestational week 35 to 36 6/7. In some embodiments, the risk of developing preeclampsia is the risk of developing preeclampsia within the following one, two, three or more weeks. In some embodiments, the risk of developing preeclampsia is the risk of developing preeclampsia within the following two weeks. In some embodiments, the PlGF-f:PlGF-d ratio is determined at least in part by: (a) determining a PlGF-f level by contacting a first PlGF probe to a first portion of the biological sample; and (b) determining a PlGF-d level by contacting the first PlGF probe to a second portion of the biological sample, wherein the first portion of the biological sample is untreated, and wherein a treatment is applied to the second portion of the biological sample wherein the treatment dissociates a PlGF complex. In some embodiments, the PlGF complex comprises PlGF and s-FLT. In some embodiments, the treatment is applied for at least 5, 10, 15, 20, 25, 30, 40, 50, 60, 90, or 120 minutes. In some embodiments, the treatment comprises increasing the temperature of the second portion of the biological sample. In some embodiments, the treatment comprises increasing the temperature to at least 30° C., at least 37° C., or at least 45° C. for at least 1 hour or at least 2 hours. In some embodiments, the treatment comprises increasing or decreasing the pH of the second portion of the biological sample. In some embodiments, the treatment comprises contacting the second portion of the biological sample with an agent that prevents PlGF from assembling into a PlGF complex. In some embodiments, the agent is selected from: a surfactant, a detergent, a chaotropic agent, a PlGF-binding protein, and an s-FLT-binding protein. In some embodiments, the first unmodified portion of the biological sample has not been stored at a temperature above about 20° C. In some embodiments, the method has a LLOQ for PlGF of 20 pg/ml or less. In some embodiments, the method further comprises (a) determining a level of at least one secondary biomarker selected from total sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274; and (b) determining the pregnant woman as being at risk or not at risk of preeclampsia by application of a trained algorithm to the PlGF-f:PlGF-d ratio in combination with the determined level of the at least one secondary biomarker. In some embodiments, the trained algorithm comprises a logistic regression, random forest, nearest centroid, gradient boosting method, linear discriminant analysis, neural network, or support vector machine algorithm. In some embodiments, the level of the at least one secondary biomarker is determined by an immunoassay. In some embodiments, the immunoassay is selected from a capture ELISA, an indirect ELISA, a TR-FRET assay, a proximity extension assay, an amplified luminescent proximity (LOCI) assay, a luminescent oxygen channeling immunoassay, or a lateral flow assay. In some embodiments, the method further comprises proceeding with treatment of the pregnant human in a manner that avoids unnecessary treatment of preeclampsia comprises administering an anti-hypertensive drug or repeating (a) at a particular frequency. In some embodiments, the method further comprises administering an anti-hypertensive drug, wherein the antihypertensive drug is a central alpha agonist, a vasodilator, a calcium-channel blocker, an alpha-blocker, acetylsalicylic acid, or a beta-blocker. - In some aspects, the present disclosure provides for a method of determining whether a pregnant human female is at risk of suffering from preeclampsia comprising: (a) determining a level of at least PlGF-f. In some embodiments, a PlGF-f level less than a first threshold value indicates a heightened risk that the pregnant human female will suffer from preeclampsia. In some embodiments, a PlGF-f level greater than a second threshold value indicates a reduced risk that the pregnant human female will suffer from preeclampsia. In some embodiments, the first threshold value and the second threshold value are the same value. In some embodiments, the method further comprises determining a level of PlGF-d. In some embodiments, the method further comprises determining a ratio of PlGF-f to PlGF-d. In some embodiments, a ratio of PlGF-f to PlGF-d less than a first threshold value indicates a reduced risk the pregnant human female will suffer from preeclampsia. In some embodiments, a ratio of PlGF-f to PlGF-d greater than a second threshold value indicates an increased risk the pregnant human female will suffer from preeclampsia. In some embodiments, the method comprises collecting the biological sample. In some embodiments, the biological sample is a fluid, whole blood, peripheral blood, serum, plasma, urine, or amniotic fluid sample. In some embodiments, the method further comprises collecting the biological sample via venipuncture, fingerstick sampling, or arterial sampling. In some embodiments, the biological sample is collected from the pregnant woman at
gestational week 20 or later. In some embodiments, the biological sample is collected from the pregnant woman atgestational week 28 or later. In some embodiments, the biological sample is collected from the pregnant woman atgestational week 35 or later. In some embodiments, the biological sample is collected from the pregnant woman prior togestational week 30. In some embodiments, the biological sample is collected from the pregnant woman prior togestational week 37. In some embodiments, the biological sample is collected from the pregnant woman atgestational week 28 to 36 6/7. In some embodiments, the biological sample is collected from the pregnant woman atgestational week 35 to 36 6/7. In some embodiments, the risk of developing preeclampsia is the risk of developing preeclampsia within the following one, two, three or more weeks. In some embodiments, the risk of developing preeclampsia is the risk of developing preeclampsia within the following two weeks. In some embodiments, the PlGF-f:PlGF-d ratio is determined at least in part by: (a) determining a PlGF-f level by contacting a first PlGF probe to a first portion of the biological sample; and (b) determining a PlGF-d level by contacting the first PlGF probe to a second portion of the biological sample, wherein the first portion of the biological sample is untreated, and wherein a treatment is applied to the second portion of the biological sample wherein the treatment dissociates a PlGF complex. In some embodiments, the PlGF complex comprises PlGF and s-FLT. In some embodiments, the treatment is applied for at least 5, 10, 15, 20, 25, 30, 40, 50, 60, 90, or 120 minutes. In some embodiments, the treatment comprises increasing the temperature of the second portion of the biological sample. In some embodiments, the treatment comprises increasing the temperature to at least 30° C., at least 37° C., or at least 45° C. for at least 1 hour or at least 2 hours. In some embodiments, the treatment comprises increasing or decreasing the pH of the second portion of the biological sample. In some embodiments, the treatment comprises contacting the second portion of the biological sample with an agent that prevents PlGF from assembling into a PlGF complex. In some embodiments, the agent is selected from: a surfactant, a detergent, a chaotropic agent, a PlGF-binding protein, and an s-FLT-binding protein. In some embodiments, the first unmodified portion of the biological sample has not been stored at a temperature above about 20° C. In some embodiments, the method has a LLOQ for PlGF of 20 pg/ml or less. In some embodiments, the method further comprises (a) determining a level of at least one secondary biomarker selected from total sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274; and (b) determining the pregnant woman as being at risk or not at risk of preeclampsia by application of a trained algorithm to the PlGF-f:PlGF-d ratio in combination with the determined level of the at least one secondary biomarker. In some embodiments, the trained algorithm comprises a logistic regression, random forest, nearest centroid, gradient boosting method, linear discriminant analysis, neural network, or support vector machine algorithm. In some embodiments, the level of the at least one secondary biomarker is determined by an immunoassay. In some embodiments, the immunoassay is selected from a capture ELISA, an indirect ELISA, a TR-FRET assay, a proximity extension assay, an amplified luminescent proximity (LOCI) assay, a luminescent oxygen channeling immunoassay, or a lateral flow assay. In some embodiments, the method further comprises proceeding with treatment of the pregnant human in a manner that avoids unnecessary treatment of preeclampsia comprises administering an anti-hypertensive drug or repeating (a) at a particular frequency. In some embodiments, the method further comprises administering an anti-hypertensive drug, wherein the antihypertensive drug is a central alpha agonist, a vasodilator, a calcium-channel blocker, an alpha-blocker, acetylsalicylic acid, or a beta-blocker. - In some aspects, the present disclosure provides for a method of treating a pregnant human female comprising the steps of: determining whether the pregnant human female has an elevated risk of preeclampsia or reduced risk of preeclampsia by: obtaining a biological sample from the pregnant human female, determining a PlGF-f:PlGF-d ratio in the biological sample, and optionally determining a level of one or more secondary biomarkers in the biological sample; applying a classifier to the PlGF-f:PlGF-d ratio and, optionally, the level of one or more secondary biomarkers to classify the pregnant human female as having an elevated risk of preeclampsia or a reduced risk of preeclampsia; if the pregnant human female has an elevated risk of preeclampsia, then determining the blood pressure of the pregnant human female at a frequency of at least one time per week, and if the pregnant human female has a reduced risk of preeclampsia, then determining the blood pressure of the pregnant human female at a frequency of less than one time per week. In some embodiments, the pregnant human female is determined to not have an elevated risk of preeclampsia, then administering an antihypertensive drug to the pregnant human female. In some embodiments, the antihypertensive drug is a central alpha agonist, a vasodilator, a calcium-channel blocker, an alpha-blocker, acetylsalicylic acid, or a beta-blocker. In some embodiments, the antihypertensive drug is methyldopa, labetalol, nifedipine, verapamil, clonidine, hydralazine, diazoxide, prazosin, or oxprenolol. In some embodiments, the method further comprises administering a cervical ripening or labor-inducing agent to the pregnant woman when the pregnant woman is determined to be at risk of suffering from preeclampsia. In some embodiments, the cervical ripening or labor-inducing agent comprises a prostaglandin, oxytocin, misoprostol, mifepristone, or relaxin.
- In some aspects, the present disclosure provides for a method of treating a pregnant human female comprising the steps of: determining whether the pregnant human female has an elevated risk of preeclampsia or reduced risk of preeclampsia by: obtaining a biological sample from the pregnant human female, determining a level of at least PlGF-f in the biological sample, and optionally determining a level of one or more secondary biomarkers in the biological sample. In some embodiments, the method further comprises applying a classifier to the PlGF-f level and, optionally, the level of one or more secondary biomarkers to classify the pregnant human female as having an elevated risk of preeclampsia or a reduced risk of preeclampsia. In some embodiments, the method further comprises determining a level of PlGF-d and applying a classifier to the PlGF-d level. In some embodiments, if the pregnant human female has an elevated risk of preeclampsia, then the method comprises determining the blood pressure of the pregnant human female at a frequency of at least one time per week. In some embodiments, if the pregnant human female has a reduced risk of preeclampsia, then the method further comprises determining the blood pressure of the pregnant human female at a frequency of less than one time per week. In some embodiments, when the pregnant human female is determined to not have an elevated risk of preeclampsia, then the method further comprises administering an antihypertensive drug to the pregnant human female. In some embodiments, the antihypertensive drug is a central alpha agonist, a vasodilator, a calcium-channel blocker, an alpha-blocker, acetylsalicylic acid, or a beta-blocker. In some embodiments, the antihypertensive drug is methyldopa, labetalol, nifedipine, verapamil, clonidine, hydralazine, diazoxide, prazosin, or oxprenolol. In some embodiments, the method further comprises administering a cervical ripening or labor-inducing agent to the pregnant woman when the pregnant woman is determined to be at risk of suffering from preeclampsia. In some embodiments, the cervical ripening or labor-inducing agent comprises a prostaglandin, oxytocin, misoprostol, mifepristone, or relaxin.
- In some aspects, the present disclosure provides for a method for avoiding unnecessary treatment of preeclampsia, the method comprising: (a) contacting a biological sample that has been collected from a pregnant human female with a plurality of different antigen-specific probes to determine levels of two or more markers, wherein the two or more markers comprise at least PlGF-f and PlGF-d; and (b) proceeding with treatment of the pregnant human in a manner that avoids unnecessary treatment of preeclampsia based at least in part on the amounts or concentrations of the markers determined in (a). In some embodiments, the two or more markers comprise three or more markers further comprising at least one of sFLT, Endoglin, KIM-1, FGF21, or free sFLT.
- In some aspects, the present disclosure provides for a system for ruling out preeclampsia in a pregnant female subject for a specified period of time, the system comprising: a processor; an input module for inputting levels of at least two markers in a biological sample, wherein the at least two markers comprise at least PlGF-f and PlGF-d; and a computer readable medium containing instructions that, when executed by the processor, performs a first algorithm on the input levels of the at least two markers. In some embodiments, the at least two markers comprise at least three markers that further comprise at least one of sFLT, Endoglin, KIM-1, FGF21, or free sFLT. In some embodiments, the system further comprises an output module providing one or more indicia based on the input levels of the at least three markers, wherein the one or more indicia are indicative of the subject not having preeclampsia for at least a specified period of time.
- In some aspects, the present disclosure provides for a method for determining levels of free and dissociated PlGF in a biological sample from a subject, the method comprising: (a) isolating a first aliquot of the biological sample for the detection of PlGF-f and a second aliquot of the biological sample for the detection of PlGF-d; (b) determining an amount of PlGF-f in the first aliquot; (c) applying a treatment to the second aliquot to dissociate PlGF complexes; and (d) determining an amount of PlGF-d in the second aliquot. In some embodiments, the method further comprises determining a ratio of the amounts of PlGF-f and PlGF-d. In some embodiments, the biological sample has not been stored at a temperature above about 20° C. In some embodiments, the amount of PlGF-f and the amount of PlGF-d are determined using same reagents. In some embodiments, the PlGF complex comprises PlGF and s-FLT. In some embodiments, the treatment is applied for at least 5, 10, 15, 20, 25, 30, 40, 50, 60, 90, or 120 minutes. In some embodiments, the treatment comprises increasing the temperature of the second portion of the biological sample. In some embodiments, the treatment comprises increasing the temperature to at least 30° C. for at least 1 hour. In some embodiments, the treatment comprises increasing or decreasing the pH of the second portion of the biological sample. In some embodiments, the treatment comprises contacting the second portion of the biological sample with an agent that prevents PlGF from assembling into a PlGF complex or disables PlGF from a PlGF:sFLT1 complex. In some embodiments, the agent is selected from: a surfactant, a detergent, a chaotropic agent, a PlGF-binding protein, and an s-FLT-binding protein. In some embodiments, the method has a LLOQ for PlGF of 20 pg/ml or less.
- In some aspects, the present disclosure provides for a method for determining free and dissociated PlGF amounts in a biological sample comprising PlGF, the method comprising: (a) determining an amount of PlGF-f in a first aliquot of the biological sample by: (i) contacting the first aliquot with a capture reagent for a first time at a first temperature, wherein the PIGF-f binds to the capture reagent to form a PIGF-f-capture reagent complex; (ii) contacting the PIGF-f-capture reagent complex with a detector reagent for a second time at a second temperature, wherein the PIGF-f-capture reagent complex binds to the second reagent to form a first ternary complex comprising PIGF-f, the capture reagent, and the detector reagent; and (iii) detecting the first ternary complex; and (b) determining an amount of PlGF-d in a second aliquot of the biological sample by: (i) contacting the second aliquot with the capture reagent for a third time at a third temperature, wherein the PIGF-d binds to the capture reagent to form a PIGF-d-capture reagent complex; (ii) contacting the PIGF-d-capture reagent complex with the detector reagent for a fourth time at a fourth temperature, wherein the PIGF-d-capture reagent complex binds to the second reagent to form a second ternary complex comprising PIGF-d, the capture reagent, and the detector reagent; and (iii) detecting the second ternary complex; wherein a PlGF-s-Flt1 complex is stable at the first temperature and the PlGF-s-Flt1 complex dissociates at the third temperature. In some embodiments: (a) the first temperature is less than 30° C., less than 25° C., between 15° C. and 30° C., between 15° C. and 25° C., between 20° C. and 25° C., or about 22° C.; (b) the second temperature is less than 30 C, less than 25 C, between 15 C and 30 C, between 15° C. and 25° C., between 20° C. and 25° C., or about 22° C.; (c) the third temperature is at least 30° C., at least 35° C., between 30° C. and 45° C., about 30° C., about 37° C. or about 45° C.; (d) the fourth temperature is at least 30° C., at least 35° C., between 30° C. and 45° C., about 30° C., about 37° C. or about 45° C.; (e) the first time is less than 1 hour, less than 45 minutes, between 10 minutes and 30 minutes, about 30 minutes, about 20 minutes, or about 15 minutes; (f) the second time is less than 1 hour, less than 45 minutes, between 10 minutes and 30 minutes, about 30 minutes, about 20 minutes, or about 15 minutes; (g) the third time is at least 1 hour, between 1 hour and 3 hours, between 90 minutes and 150 minutes, or about 120 minutes; or (h) the fourth time is at least 1 hour, between 1 hour and 3 hours, between 90 minutes and 150 minutes, or about 120 minutes. In some embodiments, the first time is about 30 minutes, the second time is about 30 minutes, the third time is about 2 hours, the fourth time is about 2 hours, the first temperature is about 22° C., the second temperature is about 22° C., the third temperature is about 37° C., and the fourth temperature is about 37° C. In some embodiments, step b is conducted in the presence of a blocking antibody that prevents s-FLT from rebinding to PlGF. In some embodiments, the biological sample is blood, serum, plasma, amniotic fluid, or urine. In some embodiments, the biological sample is serum or plasma. In some embodiments, the biological sample is collected from a pregnant human female. In some embodiments, the pregnant human female has been pregnant for at least 20 weeks. In some embodiments, the pregnant human female has been pregnant for no more than 30 weeks. In some embodiments, the biological sample is stored at a storage temperature and the storage temperature never exceeds 30° C. In some embodiments, the storage temperature is less than 0° C. In some embodiments, the storage temperature is about −80° C. In some embodiments, the biological sample is diluted prior to step a. In some embodiments, the capture reagent comprises an antibody or an antigen-binding fragment thereof. In some embodiments, the detector reagent comprises an antibody or an antigen-binding fragment thereof. In some embodiments, the capture reagent is attached to a solid support. In some embodiments, the detector reagent is attached to a solid support. In some embodiments, the detector reagent comprises a detectable label. In some embodiments, the label is a fluorophore, an enzyme, or an electrochemiluminescent tag. In some embodiments, (a) further comprises contacting the first aliquot with a crosslinking agent that prevents the dissociation of a PlGF-sFLT complex.
- In some aspects, the present disclosure provides for a method for determining a PlGF-f:PlGF-d ratio in a biological sample, wherein PlGF-f is determined using a reagent that can bind to PlGF-f but cannot bind to PlGF-d. In some embodiments, the reagent is an antibody or antigen-binding fragment thereof. In some embodiments, the reagent binds to residues on the PlGF-1 molecule that are involved in recognition of sFlt-1.
- In some aspects, the present disclosure provides for a method for determining a PlGF-f:PlGF-d ratio in a biological sample, wherein PlGF-d is determined using a reagent that can bind to PlGF-d but cannot bind to PlGF-f. In some embodiments, the reagent is an antibody or antigen-binding fragment thereof. In some embodiments, the reagent binds to an interface between PlGF and sFlt-1.
- In some aspects, the present disclosure provides for a method for determining free and dissociated PlGF amounts in a biological sample comprising separating the biological sample into a free PlGF component and a PlGF complex component, determining the amount of PlGF in the free PlGF component, and determining the amount of PlGF in the PlGF complex component. In some embodiments, the separating is performed by anion exchange chromatography, cation exchange chromatography, gel filtration chromatography or filtration. In some embodiments, the biological sample is contacted with a chemical crosslinking agent prior to the separating.
- In some aspects, the present disclosure provides for a kit for assessing a risk of preeclampsia or ruling out preeclampsia in a pregnant female subject, the kit comprising: (a) an agent or apparatus for dissociating placental growth factor (PLGF) from a PLGF complex with another protein; and (b) at least one probe for specific binding to placental growth factor (PlGF). In some embodiments, the kit is designed to measure the levels of no more than 20, no more than 15, no more than 10, nor more than 8, no more than 7, no more than 6, nor more than 5, or no more than 4 proteins. In some embodiments, the kit is further designed to measure the levels of at least one of sFLT, Endoglin, KIM-1, FGF21, or free sFLT In some embodiments, the kit further comprises at least one probe for specific binding to at least one of sFLT, Endoglin, KIM-1, FGF21, or free sFLT In some embodiments, the kit further comprises instructions for carrying out an immunoassay. In some embodiments, the kit is an enzyme-linked immunosorbent assay kit. In some embodiments, one or more of the probes are attached to a substrate. In some embodiments, the kit is for a lateral flow immunoassay. In some embodiments, the kit further comprises a collection tube for collecting a biological sample from the pregnant female subject. In some embodiments, the collection tube is a collection tube suitable for collection of serum or plasma. In some embodiments, the at least one probe for specific binding to PlGF or the least one probe for specific binding to at least one of sFLT, Endoglin, KIM-1, FGF21, or free sFLT comprises an antibody or antigen-binding fragment or derivative thereof. In some embodiments, the kit further comprising a microwell or multiwell plate. In some embodiments, the microwell or multiwell plate comprises at least one well with the at least one probe for specific binding to PlGF or the least one probe for specific binding to at least one of sFLT, Endoglin, KIM-1, FGF21, or free sFLT conjugated to a surface therein.
- All publications, patents, and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication, patent, or patent application was specifically and individually indicated to be incorporated by reference.
- The novel features of the invention are set forth with particularity in the appended claims. A better understanding of the features and advantages of the present invention will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the invention are utilized, and the accompanying drawings, which are summarized below. In these figures and the subsequent detailed description, PlGF(f) or PlGF-f refers to free Placental Growth Factor (PlGF), and PlGF(d) or PlGF-d refers to disassociated PlGF as defined herein.
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FIGS. 1A and 1B depict schemes showing the species of PlGF predicted to be present in pregnant patient serum samples (A), as well as the predicted behavior of these species in sandwich ELISA assays (B). PlGF species can include free PlGF (e.g., in dimerized form), PLGF(2:1) (e.g., PlGF dimer and single sFLT molecule), and PlGF(2:2) (e.g., PlGF dimer and two sFLT molecules). -
FIGS. 2A and 2B illustrate the change in measured PlGF concentration of PE and Non-PE samples incubated at various capture/detection temperatures (A), as well as the predicted behavior of different PlGF species during sample capture and detection incubation (B). -
FIGS. 3A and 3B illustrate the effects of various capture temperatures and sFLT concentrations on the detection of PlGF using 3rd trimester pregnancy samples. -
FIGS. 4A, 4B, 4C, and 4D illustrate the effects of various capture times and sFLT concentrations on the detection of PlGF using 3rd trimester pregnancy samples. -
FIGS. 5A and 5B illustrate a pilot study of where PlGF was measured at various temperatures—22° C. for PlGF(f), 30° C. for PlGF(II), and 37° C. for PlGF(d)—and in the presence of various amounts of sFLT in serum from patients with and without preeclampsia. -
FIG. 6 shows plots of PE and non-PE patients from the studies segregated using PlGF(f) conc or % free PlGF ((f)/(d)×100) demonstrating that both metrics separate PE samples from non-PE samples and % free PlGF shows improved separation. In this figure blue dot indicates Non-PE, orange dot indicates PE, grey dot indicates PE from 104 cohort, and * indicates below LLOQ. -
FIGS. 7A, 7B, and 7C show PlGF measurements of a larger set of PE and non-PE serum samples using different protocols for measuring species of PlGF—22 C for PlGF(f), 30 C for PlGF(II), and 37 C for PlGF(d)—and in the presence of various amounts of sFLT, demonstrating that % free PlGF can improve separation of PE from non-PE samples. -
FIG. 8 illustrates determination of performance metrics for PlGF(f), PlGF(d), and PlGF (% f) alongside the old PlGF protocol (“PlGF(II)” as markers to discriminate PE from non-PE patients. -
FIG. 9 illustrates a calibration curve using recombinant PlGF using the optimized conditions described in Example 2 which was used to estimate LLOQ for PlGF(f) (10 pg/ml) and PlGF(d) (20 pg/ml) species. -
FIG. 10 illustrates analysis of PlGF(d), PlGF(f), and PlGF (% free) versus gestational age (GA), showing that free PlGF offers a robust segregation into earlier gestational age (<28 weeks), indicating that free PlGF (or modifications thereof) could further enable stratification of the preeclampsia cohort at earlier gestational ages. The separation of non-PE and PE lines for dissociated PlGF, free PlGF, and % free PlGF indicates that the ratio of free to dissociated PlGF can allow for normalization within a patient while maintaining separation between non-PE and PE samples. Further, it appears low dissociated PlGF may identify a subset of patients with a different risk profile. -
FIG. 11 shows an exemplary computer system that can be used alongside the methods, compositions, and systems described herein. -
FIG. 12 shows an AUC analysis for a univariate model based on % free PlGF as described in Example 5. -
FIG. 13 depicts cutoff parameters for the univariate model based on % free PlGF as described in Example 5. -
FIG. 14 depicts inclusion/exclusion criteria for the PRO-129 study used to validate the models and clinical profile/model combinations described herein. -
FIGS. 15A and 15B show the different definitions of clinical categories used in cross-sectional and forward-looking “rule out” analyses performed in Examples 5 and 7. - Detection of preeclampsia using the classic clinical symptoms of the disease is error-prone, risking significant adverse outcome for patients and an added burden to the healthcare system through misdiagnosis. Measurement of proteinuria is prone to inaccuracies (e.g., false negatives and false positives), preeclampsia complications may occur before proteinuria becomes significant, the clinical presentation of preeclampsia can be highly variable in progression rate, onset time, and severity, and the symptoms (hypertension, proteinuria, or both) can be indicative of other distinct disorders that could utilize a less aggressive treatment course (chronic hypertension, gestational hypertension, temporary high blood pressure, and gestational diabetes). Thus, there is significant risk for patients with only suspected preeclampsia to be over treated (e.g., delivered or induced early, thereby unnecessarily putting the infant at risk of preterm birth complications and/or unnecessarily putting the mother at risk of surgical complications), and there is risk for patients with silent or rapidly-progressing preeclampsia to be treated insufficiently aggressively, thereby putting the infant at risk of intrauterine growth restriction or death, and the mother at risk of preeclamptic sequelae such as eclampsia seizures, renal or liver damage, pulmonary edema, placental abruption, and coma).
- Application of the sFlt1/PlGF ratio in patient serum as a method for identifying preeclampsia would seem to mitigate these problems, except that this method has limited sensitivity and specificity (see e.g., Zeisler et al. NEJM 274(2017):13-22 or Verlohren et al. Hypertension. 63(2014):346-52, where the method was identified as having maximal sensitivity of 80% and specificity of 78.3%) and thus involves a significant proportion of false negatives and false positives, making it of limited use in ruling out a diagnosis of preeclampsia and avoiding the overtreatment/under treatment conundrum.
- Accordingly, there is a need for the methods, compositions, systems and kits for improved detection or prediction of preeclampsia in pregnant patients, particularly those that enable the detection or prediction of preeclampsia with a high negative predictive value and/or allow medical professionals to rule out a diagnosis of preeclampsia for an extended period of time.
- As used in the specification and in the claims, the singular form “a,” “an,” and “the” include plural referents unless the context clearly dictates otherwise.
- The term “hypertension” refers to abnormally high blood pressure. Hypertension can be identified in any suitable manner, such as by reference to a sitting systolic blood pressure (sSBP) of greater than 140 mmHg or a sitting diastolic blood pressure (sDBP) of greater than 90 mmHg. Hypertension can be further classified into
class 1 orclass 2 hypertension, withclass 1 exhibiting sSBP of 140-149 mmHg or 90-99 mmHg sDBP, andclass 2 exhibiting greater than 160 mmHg sSBP or 100 mmHg sDBP. (See, e.g., The Seventh Report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure. JAMA 2003; 289:2560-71.) Other suitable criteria may be used to identify hypertension, such as having a sitting systolic blood pressure of greater than 130 and/or a sitting diastolic blood pressure of greater than 90 mmHg. Hypertension can also be determined according to the 2017 AHA guidelines (see Whelton et al. 2017 ACC/AHA/AAPA/ABC/ACPM/AGS/APhA/ASH/ASPC/NMA/PCNA Guideline for the Prevention, Detection, Evaluation, and Management of High Blood Pressure in Adults, Journal of the American College of Cardiology (2017), doi: 10.1016/j.jacc.2017.11.006). Such guidelines identify “normal” blood pressure as less than 120/80 mmHg, “elevated” as systolic between 120-129 mmHg and diastolic less than 80 mmHg, “stage 1” as systolic between 120-139 mmHg or diastolic between 80-89 mmHg, “stage 2” as systolic at least 140 mmHg or diastolic at least 90 mmHg, and “hypertensive crisis” as systolic over 180 and/or diastolic over 120. - As used herein, the term “proteinuria” is defined as the presence of abnormal protein in the urine. A number of indicator dyes and reagents can used to measure proteinuria semi-quantitatively (e.g., bromophenol blue). In some embodiments, concentrations of protein in urine can be determined by a semi quantitative “dipstick” analysis and graded as negative, trace (10-20 mg/dL), 1+(˜30 mg/dL), 2+(˜100 mg/dL), 3+(˜300 mg/dL), or 4+(1,000 mg/dL), with 2+ commonly being used as the threshold for problematic proteinuria. In an alternative scheme, concentrations of protein in urine can also be measured per 24-hour urine collection, in which greater than or equal to 300 mg protein indicates proteinuria. In an alternative scheme, concentrations of protein in urine can be measured in a spot urine sample, in which 30 mg of protein per deciliter or greater indicates proteinuria. In yet an alternative scheme, proteinuria can also be expressed as the protein/creatinine ratio (Pr/Cr) in urine, in which a Pr/Cr ratio of >0.3 is indicative of problematic proteinuria.
- As used herein, the term “antibody or fragments thereof” is used in the broadest sense and specifically encompasses intact monoclonal antibodies, polyclonal antibodies, multispecific antibodies (e.g. bispecific antibodies formed from at least one binding domain of two intact antibodies), and antibody fragments. Antibody fragments comprise a portion of an intact antibody that retains antigen-binding activity; examples include Fab, Fab′, F(ab)2, F(abc)2, and Fv fragments as well as diabodies, linear antibodies, scFvs, and multispecific antibodies formed from antibody fragments.
- As used herein, the term “preeclampsia” (“PreE” or “PE”) refers to a pregnancy-specific disorder involving multiple organ systems thought to originate from abnormal placentation, dysfunctional trophoblast development, defective placental angiogenesis, and a heightened systemic inflammatory response in the mother. Preeclampsia, when untreated, can progress to ecclampsia, HELLP syndrome, hemorrhagic or ischemic stroke, liver damage, acute kidney injury, and acute respiratory distress syndrome (ARDS). Thus, while preeclampsia frequently presents with symptoms such as hypertension and proteinuria, it is distinct from simple vascular tension disorders and kidney dysfunction (as evidenced by the non-overlapping set of complications that result when it is untreated), and symptoms reflective of vascular tension disorders and kidney dysfunction on their own therefore have less than ideal predictive/diagnostic value for preeclampsia. Further information on the pathophysiology of preeclampsia can be found, e.g., in Phipps et al. Clin J Am Soc Nephrol 11(2016):1102-1113. In some embodiments, a traditional diagnosis of preeclampsia is made when hypertension and proteinuria as defined above are detected at the same time. In other embodiments (see American College of Obstetricians and Gynecologists; Task Force on Hypertension in Pregnancy. Obstet Gynecol. 122(2013):1122-31, which is explicitly incorporated by reference herein) a traditional diagnosis of preeclampsia is made upon simultaneous detection of hypertension (blood pressure greater than or equal to 140 mmHg systolic or greater than or equal to 90 mmHg diastolic on two occasions at least 4 hours apart after 20 weeks gestation in a woman with a previously normal blood pressure, or blood pressure with greater than equal to 160 mmHg systolic or greater than or equal to 110 mmHg diastolic within a short interval of minutes) and proteinuria (greater than or equal to 300 mg per 24-hour urine collection either measured or extrapolated, or protein creatinine ratio greater than or equal to 0.3, or a dipstick reading of 1+(pre-2013 guidelines) or 2+(post-2013 guidelines))) or upon new onset hypertension in the absence of proteinurea when (a) blood platelet count is less than 100,000 per milliliter, (b) serum creatinine is greater than 1.1 mg/dL or double baseline for the patient, or (c) blood concentration of liver transaminases is twice normal or greater). In some cases, preeclampsia is made without a diagnosis of proteinurea, for instance where evidence of other end-stage organ damage is present.
- The term “subject” can include human or non-human animals. Thus, the methods and described herein are applicable to both human and veterinary disease and animal models. Preferred subjects are “patients,” i.e., living humans that are receiving medical care for a disease or condition. This includes persons with no defined illness who are being investigated for signs of pathology.
- The term “unnecessary treatment of preeclampsia” can refer to treatments for preeclampsia that would be, statistically speaking, unjustified when a practitioner considers the results of a test or procedure described herein, such as a test based on the determination of an amount of concentration of various protein biomarkers. Such unnecessary treatment can include preterm induction based on one or more symptoms or risk factors for preeclampsia.
- The term PlGF refers to Placental Growth Factor. PlGF is also referred to as PlGF or PIGF and is encoded by the PGF gene. PlGF includes at least four known isoforms: PlGF-1, PlGF-2, PlGF-3, and PlGF-4.
- The term sFLT or sFLT1 refers to the soluble ectodomain of Fms-
like tyrosine kinase 1, also known as Vascular EndothelialGrowth Factor Receptor 1, FLT-1, FLT1, FRT, or VEGFR1. The term “free PlGF” (also “PlGF(f)” or “PlGF-f”) refers to PlGF in a biological sample not complexed another other binding partner. In some embodiments, the other binding partner is sFLT1, with which it can form at least two complexes with PlGF: 2:2 sFLT:PlGF and 2:1 sFLT:PlGF complexes. - The term “dissociated PlGF” (also “PlGF(d)” or “PlGF-d”) refers to PlGF that has been dissociated from its binding partners in a biological sample by subsequent in vitro treatment. In some embodiments, the other binding partner is sFLT1, with which it can form at least 2:2 sFLT:PlGF and 2:1 sFLT:PlGF complexes. In some embodiments, when treatment is sufficient to dissociate substantially all PlGF complexes with another protein (such as sFLT1), “dissociate PlGF” is equal to “total PlGF” (e.g. the total level of PlGF present in the biological sample).
- Subjects
- In some embodiments, the methods, compositions, systems and kits provided herein are used for detecting or predicting a condition in a pregnant human subject at any stage in pregnancy. In other embodiments, the pregnant human subject is post-the 20th week of gestation. In other embodiments, the pregnant human subject is post-first or -second trimester of pregnancy. In some embodiments, the pregnant human subject is post- or equal to the 13th, 14th, 15th, 16th, 17th, 18th, 19th, 20th, 21st, 22nd, 23rd, 24th, 25th, 26th, 27th, 28th, 29th, 30th, 31st, 32nd, 33rd, 34th, 35th, 36th, 37th, 38th, 39th, 40th, 41st, or 42nd week of gestation, or any range between these values.
- The methods, compositions, systems and kits are suitable for detecting or predicting a condition of the pregnant subject such as preeclampsia (PreE) or non-preeclampsia (NonPreE). In some embodiments, preeclampsia is further divided into very early-onset (before 25 weeks gestation), early-onset (before 34 weeks gestation) and late-onset (after 34 weeks gestation) preeclampsia. Typically, when the pregnant patient does not exhibit hypertensive or renal symptoms, the patient is considered NonPreE. Further, when the pregnant patient exhibits only hypertensive symptoms alone without signs of proteinuria, the patient is generally only suspected to have preeclampsia. However, as the measurement of proteinuria is prone to inaccuracies (e.g., false negatives and false positives), preeclampsia complications may occur before proteinuria becomes significant, the clinical presentation of preeclampsia can be highly variable (from severe, rapidly progressing, and early-onset to late-onset and less severe), and the symptoms (hypertension, proteinuria, or both) can be indicative of other distinct disorders that could utilize a different treatment course. Thus, there is significant risk for patients with only suspected preeclampsia to be unnecessarily treated (e.g., delivered/induced early putting the infant at risk of preterm birth complications or the mother at risk of surgical complications), and there is risk for patients with silent or rapidly-progressing preeclampsia to be treated insufficiently aggressively (e.g., putting the infant at risk of intrauterine growth restriction or death, and the mother at risk of preeclampsia sequelae such as eclampsia seizures, renal or liver damage, pulmonary edema, placental abruption, and coma).
- A subject therefore can be a pregnant female that has no known risk factors or has one or more at-risk factors for a condition such as PreE. In some instances, hypertension and/or proteinuria can indicate a subject at risk of preeclampsia. In some instances, a subject at risk of preeclampsia can have a urine protein content measured as 2+(100 mg/dL) or greater by dipstick assay, greater than or equal to 300 mg per 24-hour urine collection, 30 mg of protein per deciliter or greater in a spot urine sample, or a Pr/Cr ratio in urine of >30 mg per millimole. In some instances, a subject at risk of preeclampsia can have a sitting systolic blood pressure (sSBP) of greater than 140 mmHg or a sitting diastolic blood pressure (sDBP) of greater than 90 mmHg or both. In some instances, sFlt1/PlGF ratio can be used to identify subject at risk of preeclampsia (see, e.g., Zeisler et al. NEJM 274(2017):13-22 or Verlohren et al. Hypertension. 63(2014):346-52). In some instances, both hypertension and proteinuria can be used to identify a subject at risk of preeclampsia. In some instances, new onset hypertension in combination with one or more symptoms selected from (a) blood platelet count is less than 100,000 per milliliter, (b) serum creatinine is greater than 1.1 mg/dL or double baseline for the patient, or (c) blood concentration of liver transaminases is twice normal or greater can be used to identify a subject at risk of preeclampsia.
- Samples
- Methods for detecting molecules (e.g., nucleic acids, proteins, etc.) in a pregnant subject in order to detect, diagnose, monitor, predict, or evaluate the status or outcome of the pregnancy are described in this disclosure. In some cases, the molecules are circulating molecules (e.g. unbound to cells and freely circulating in bodily fluids such as blood, blood plasma or blood serum). In some cases, the molecules are expressed in the cytoplasm of blood, endothelial, or organ cells. In some cases, the molecules are expressed on the surface of blood, endothelial, or organ cells.
- The methods, kits, and systems disclosed herein can be used to classify one or more samples from one or more subjects. A sample can be any material containing tissues, cells, nucleic acids, genes, gene fragments, expression products, proteins, polypeptides, exosomes, gene expression products, or gene expression product fragments of a subject to be tested. A sample can include but is not limited to, tissue, cells, plasma, serum, or any other biological material from cells or derived from cells of an individual. The sample can be a heterogeneous or homogeneous population of cells or tissues. The sample can be a fluid that is acellular or depleted of cells (e.g., plasma or serum). In some cases, the sample is from a single patient. In some cases, the method comprises analyzing multiple samples at once, e.g., via massively parallel multiplex expression analysis on protein arrays or the like.
- The sample is preferably a bodily fluid. The bodily fluid can be saliva, urine, blood, and/or amniotic fluid. The sample can be a fraction of any of these fluids, such as plasma, serum or exosomes (exemplary exosome isolation techniques can be found, e.g. in Li et al. Theranostics. 7(2017): 789-804). In preferred embodiments, the sample is a blood sample, plasma sample, or serum sample.
- The sample may be obtained using any method that can provide a sample suitable for the analytical methods described herein. The sample may be obtained by a non-invasive method such as a throat swab, buccal swab, bronchial lavage, urine collection, scraping of the cervix, cervicovaginal sample secretion collection (e.g. with an ophthalmic sponge such as a Weck-Cel sponge), saliva collection, or feces collection. The sample may be obtained by a minimally-invasive method such as a blood draw. The sample may be obtained by venipuncture.
- As used herein “obtaining a sample” includes obtaining a sample directly or indirectly. In some embodiments, the sample is taken from the subject by the same party (e.g. a testing laboratory) that subsequently acquires biomarker data from the sample. In some embodiments, the sample is received (e.g. by a testing laboratory) from another entity that collected it from the subject (e.g. a physician, nurse, phlebotomist, or medical caregiver). In some embodiments, the sample is taken from the subject by a medical professional under direction of a separate entity (e.g. a testing laboratory) and subsequently provided to said entity (e.g. the testing laboratory). In some embodiments, the sample is taken by the subject or the subject's caregiver at home and subsequently provided to the party that acquires biomarker data from the sample (e.g. a testing laboratory). A variety of kits suitable for self or home collection of biological samples have been described commercially and in the literature; see e.g., US20170023446A1 and U.S. Pat. No. 4,777,964A.
- Sample Data
- The methods, kits, and systems disclosed herein may comprise data pertaining to one or more samples or uses thereof. The data can be representative of an amount or concentration of one or more biomarkers, such as various proteins described herein. Stated differently, the data can be expression level data of proteins or polypeptides. The expression level data of biomarkers described herein can be determined by protein array, proteomics, expression proteomics, mass spectrometry (e.g., liquid chromatography-mass spectrometry (LC-MS), multiple reaction monitoring (MRM), selected reaction monitoring (SRM), scheduled MRM, scheduled SRM), 2D PAGE, 3D PAGE, electrophoresis, proteomic chip, proteomic microarray, Edman degradation, direct or indirect ELISA, immunosorbent assay, immuno-PCR (see, e.g., Sano et al. Science. 258(1992):120-2.), proximity extension assay (see, e.g., Thorsen et al. Journal of Translational Medicine. 11(2013):253, US20130288249A1, U.S. Pat. No. 9,777,315B2), Luminex assay, or homogenous assays such as ALPHAscreen (see, e.g., Application Note. Nature Methods 5, (2008), U.S. Pat. Nos. 5,898,005A, 5,861,319A), time-resolved fluorescence resonance energy transfer (TR-FRET see e.g., US20130203068A1 and WO1998015830A2), time-resolved fluorescence (TRF), fluorescent oxygen channeling immunoassay (FOCI), or luminescent oxygen channeling immunoassay (LOCI™; see e.g. Ullman et al. Proc Natl Acad Sci USA. 91(1994):5426-5430 or Ullman et al. Clin Chem. 1996 September; 42(9):1518-26 for exemplary methods and reagents).
- In some embodiments, the compositions, methods and devices described herein make use of labeled molecules in various sandwich, competitive, or non-competitive assay formats to determine expression levels of biomarkers described herein. Such methods generate a signal that is related to the presence or amount of one or more of the proteins described herein. Suitable assay formats also include chromatographic, mass spectrographic, and protein “blotting” methods. Additionally, certain methods and devices, such as biosensors, optical immunoassays, immunosorbent assays, and enzyme immunoassays, may be employed to determine the presence or amount of analytes without the need for a labeled molecule. Examples of enzyme immunoassays (EIA) include chemiluminescent enzyme immunoassay, electrochemiluminescence immunoassay (ECLIA), and enzyme-linked immunosorbent assay (ELISA), which are further described by Kuhle, Jens, et al. “Comparison of three analytical platforms for quantification of the neurofilament light chain in blood samples: ELISA, electrochemiluminescence immunoassay and Simoa.” Clinical Chemistry and Laboratory Medicine (CCLM) 54.10 (2016): 1655-1661. Robotic instrumentation for performing these immunoassays are commercially available including, but not limited to Abbott AXSYM®, IMx®, or Commander® systems; Biolog 24i® or CLC480 systems; Beckman Coulter ACCESS®, ACCESS 2®, or Unicel Dxl 600/800 systems; bioMerieux VIDAS® or mini-VIDAS® systems; Chimera Biotec GmbH Imperacer® assay; Dade Behring STRATUS® system; DiaSorin LIAISON XL® or ETI-Max 300 systems; Dynex Agility® system; Gold Standard Diagnostics Thunderbolt® analyzer; Gyrolab xPlore/xPand® system; Hudson Robotics ELISA Workstation; Ortho Clinical Diagnostics Vitros® ECL or 3600 systems; Hamiltorn Robotics ELISA NIMBUS or STARlet systems; Luminex xMAP® system; PerkinElmer ALPHAscreen® or AlphaLISA®; Phadia Laboratory System 100E, 250, 1000, 2500, or 5000; Quanterix SIMOA® system; Quidel Sofia®2 POC systems; Radiometer AQT90 Flex system; Roche Diagnostics ElecSys® 2010, Cobas® 4000/6000/8000 Analyzers, or Integra® 400 Plus; c111, c311, c501, c502 family of analyzers; Seikisui Diagnostics FastPack® IP automated system; Siemens Dimension Vista® 1500 System, DPC Immulite 1000/2000 system, or DCA Vantage® Analyzer; Singulex Single Molecule Counting (SMC™) assay; Stratus® CS Acute Care Diagnostic System; Sysmex Eurolyser®; ThermoFisher MGC 240 Benchtop Analyzer; Tosoh Bioscience AIA®-360 or AIA-600II systems; UniCel Dxl 860i Synchron Access Clinical System, UniCel DxC 680i Synchron Access Clinical System, Access/Access 2 Immunoassay System, 600 Access Immunoassay System, 600i Synchron Access Clinical System, Dxl 800 Access Immunoassay System, DxC 880i Synchron Access Clinical System; and Vital Diagnostics PathFast® point-of-care chemiluminescence immunoassay analyzer. But any suitable immunoassay may be utilized, for example, enzyme-linked immunoassays (ELISA), radioimmunoassays (RIAs), competitive binding assays, and the like.
- Other exemplary analytical systems include assay systems, such as, for example, optical systems containing one or more sources of radiation and/or one more detector. Such systems may use, for example, a light source that illuminates and a sample and a detector configured to detect light that is emitted by the sample (e.g., fluorescence spectroscopy), optical density (e.g., the portion of light that passes through the sample), and/or light that is diffracted by sample (e.g., diffraction optics). An analytical system may use, for example, ELISA (enzyme-linked immunosorbent assay). An analytical system may use, for example, LOCI (luminescent oxygen channeling), FOCI (fluorescent oxygen channeling), or ALPHAscreen. An analytical technique may involve incubating and/or diluting a sample before or during the analysis/assaying of the sample.
- In some embodiments, the compositions, methods and devices described herein make use of fluorescent oxygen channeling immunoassay (FOCI) compositions and methods. FOCI is generally described in U.S. Pat. Nos. 5,807,675; 5,616,719; and 7,635,571, the entire contents of which are expressly incorporated herein by reference. In some embodiments, a first analyte-binding agent that is capable of binding to an analyte and comprises a photosensitizer is used in combination with a second analyte-binding agent comprising a fluorogenic dye. In some embodiments, the photosensitizer of the first analyte-binding agent generates singlet oxygen in an excited state thereby causing the fluorogenic dye of the second analyte-binding agent to emit fluorescence upon reacting with the singlet oxygen. In some embodiments, the emitted fluorescence can be detected to, e.g., determine the presence and/or absence of the analyte and/or to quantitate and/or analyze the analyte in a sample. In some embodiments, the first and the second analyte-binding agents bind to the same region (e.g., epitope) of the analyte (e.g., a protein). For example, in some embodiments, the first and the second analyte-binding agents comprise the same type of analyte-binding moiety or reagent (e.g., the same antibody). In some embodiments, the first and the second analyte-binding agents bind to separate regions (e.g., epitopes) of the analyte (e.g., a protein). In some embodiments, the first and the second analyte-binding agents bind to the separate regions of the analyte (e.g., a protein) that do not spatially overlap. In some embodiments, the first analyte-binding agent and the second analyte-binding agent are configured such that when both analyte-binding agents are bound to the analyte, the singlet oxygen generated by photosensitizer of the first analyte-binding agent is in close proximity to the fluorogenic dye of the second analyte-binding agent. In some embodiments, the first and/or second analyte binding agent(s) is an antigen-binding agent (e.g., an antibody). In some embodiments, the first and/or second analyte binding agent(s) is an affimer. In some embodiments, the first and/or second analyte binding agent(s) is an antigen-binding agent is an aptamer. In some embodiments, both the photosensitizer and fluorogenic dye are provided in the form of beads.
- In some cases, arrays can use different probes (e.g., antibodies, scFvs, Fab fragments) attached to different particles or beads. In such arrays, the identity of which probe is attached to which particle or beads may be determinable from an encoding system. The probes can be antibodies or antigen-binding fragments or derivatives thereof.
- The data pertaining to the sample can be compared to data pertaining to one or more control samples. In some cases, control samples can be samples from the same patient at different times. In some cases, the one or more control samples can comprise one or more samples from healthy subjects, unhealthy subjects, or a combination thereof. The one or more control samples can comprise one or more samples from healthy subjects, subjects suffering from pregnancy-associated conditions other than preeclampsia, subjects suffering chronic conditions along with pregnancy associated conditions, or subjects suffering from chronic conditions alone.
- In some instances, the expression level data for various samples is used to develop or train an algorithm or classifier provided herein. In some instances, where the subject is a patient, such as a pregnant female; gene expression levels are measured in a sample from the patient and a classifier or algorithm (e.g., trained algorithm) is applied to the resulting data in order to detect, predict, monitor, rule out, or estimate the risk of a pregnancy-associated condition such as preeclampsia.
- In some cases, analysis of expression levels initially provides a measurement of the expression level of each of several individual biomarkers. The expression level can be absolute in terms of a concentration of a biomarker, or relative in terms of a relative concentration of an expression product of interest to another biomarker in the sample. For example, relative expression levels of proteins can be expressed with respect to the expression level of a house-keeping or structural protein in the sample. Relative expression levels can also be determined by simultaneously analyzing differentially labeled samples bound to the same array. Expression levels can also be expressed in arbitrary units, for example, related to signal intensity.
- Classifiers and Classifier Probe Sets
- Disclosed herein is the use of a classification system comprising one or more classifiers. In some instances, the classifier is a 2-way classifier. In some instances, a two-way classifier can classify a sample from a pregnant patient into one of two classes comprising preeclampsia (PreE) and non-preeclampsia (nonPreE). In some instances, the classifier may be used classify a subject as not needing treatment for preeclampsia. In some instances, a multi-way classifier may be used (e.g., preeclampsia, non-preeclampsia, and indeterminate).
- Classifiers and/or classifier probe sets (e.g., antibody sets) can be used to either rule-in or rule-out a sample as from a patient to be treated for preeclampsia. For example, a classifier can be used to classify a sample as being from a healthy subject. Alternatively, a classifier can be used to classify a sample as being from an unhealthy subject. Alternatively, or additionally, classifiers can be used to either rule-in or rule-out a sample as being from a subject who should be treated for preeclampsia.
- Many statistical classification techniques are suitable to classify samples as described above. In supervised learning approaches, a group of samples from two or more groups (e.g. PE and non-PE) are analyzed with a statistical classification method. Biomarker presence/level data can be used as a classifier that differentiates between the two or more groups. A new sample can then be analyzed so that the classifier can associate the new sample with one of the two or more groups. Commonly used supervised classifiers include without limitation the neural network (multi-layer perceptron), support vector machines, k-nearest neighbors, Gaussian mixture model, Gaussian, naive Bayes, decision tree and radial basis function (RBF) classifiers. Linear classification methods include Fisher's linear discriminant, logistic regression, naive Bayes classifier, perceptron, and support vector machines (SVMs). Other classifiers for use with the invention include quadratic classifiers, k-nearest neighbor, boosting, decision trees, random forests, neural networks, pattern recognition, Bayesian networks and Hidden Markov models. One of skill will appreciate that these or other classifiers, including improvements of any of these, are contemplated within the scope of the invention.
- Classification using supervised methods is generally performed by the following methodology:
- In order to solve a given problem of supervised learning (e.g. learning to recognize handwriting) one has to consider various steps:
- 1. Gather a training set. These can include, for example, serum samples from pregnant female patients (e.g. PE and non-PE). The training samples are used to “train” the classifier.
- 2. Determine the input “feature” representation of the learned function. The accuracy of the learned function depends on how the input object is represented. Typically, the input object is transformed into a feature vector, which contains a number of features that are descriptive of the object. The number of features should not be too large, because of the curse of dimensionality; but should be large enough to accurately predict the output. The features might include a set of biomarkers as described herein.
- 3. Determine the structure of the learned function and corresponding learning algorithm. A learning algorithm (e.g. “trained algorithm”) is chosen, e.g., artificial neural networks, decision trees, Bayes classifiers or support vector machines. The learning algorithm is used to build the classifier.
- 4. Build the classifier (e.g. classification model). The learning algorithm is run on the gathered training set. Parameters of the learning algorithm may be adjusted by optimizing performance on a subset (called a validation set) of the training set, or via cross-validation. After parameter adjustment and learning, the performance of the algorithm may be measured on a test set of naive samples that is separate from the training set.
- Once the classifier (e.g. classification model) is determined as described above, it can be used to classify a sample, e.g., a serum sample from a pregnant patient that is being analyzed by the methods described herein.
- Unsupervised learning approaches can also be used with the invention. Clustering is an unsupervised learning approach wherein a clustering algorithm correlates a series of samples without the use the labels. The most similar samples are sorted into “clusters.” A new sample could be sorted into a cluster and thereby classified with other members that it most closely associates.
- Data Analysis Systems and Methods
- The methods, kits, and systems disclosed herein can comprise algorithms or uses thereof. The one or more algorithms can be used to classify one or more samples from one or more subjects. The one or more algorithms can be applied to data from one or more samples. The data can comprise biomarker expression data.
- The methods disclosed herein can comprise assigning a classification to one or more samples from one or more subjects. Assigning the classification to the sample can comprise applying an algorithm to the expression level. In some cases, the gene expression levels are inputted to a data analysis system comprising a trained algorithm for classifying the sample as one of the conditions comprising preeclampsia, eclampsia, non-preeclampsia, chronic hypertension, gestational hypertension, or HELLP (Hemolysis, Elevated Liver enzymes, and Low Platelet count—see e.g., Weinstein et al. Am J Obstet Gynecol. 142(1982):159-67) syndrome. In some embodiments the algorithm can, as part of its execution, calculate an index for a sample and compare the sample index to a threshold value; the predefined relationship can be indicative of a likelihood of the sample belonging to a particular classification.
- The algorithm can provide a record of its output including a classification of a sample and/or a confidence level. In some instances, the output of the algorithm can be the possibility of the subject of having a condition comprising preeclampsia, eclampsia, chronic hypertension, gestational hypertension, or HELLP syndrome.
- A data analysis system can be a trained algorithm. The algorithm can comprise a linear classifier. In some instances, the linear classifier comprises one or more of linear discriminant analysis, Fisher's linear discriminant, Naïve Bayes classifier, Logistic regression, Perceptron, Support vector machine, or a combination thereof. The linear classifier can be a support vector machine (SVM) algorithm. The algorithm can comprise a two-way classifier. The two-way classifier can comprise one or more decision tree, random forest, Bayesian network, support vector machine, neural network, or logistic regression algorithms.
- The algorithm can comprise one or more linear discriminant analysis (LDA), Basic perceptron, Elastic Net, logistic regression, (Kernel) Support Vector Machines (SVM), Diagonal Linear Discriminant Analysis (DLDA), Golub Classifier, Parzen-based, (kernel) Fisher Discriminant Classifier, k-nearest neighbor, Iterative RELIEF, Classification Tree, Maximum Likelihood Classifier, Random Forest, Nearest Centroid, Prediction Analysis of Microarrays (PAM), k-medians clustering, Fuzzy C-Means Clustering, Gaussian mixture models, graded response (GR), Gradient Boosting Method (GBM), Elastic-net logistic regression, logistic regression, or a combination thereof. The algorithm can comprise a Diagonal Linear Discriminant Analysis (DLDA) algorithm. The algorithm can comprise a Nearest Centroid algorithm. The algorithm can comprise a Random Forest algorithm. In some embodiments, for discrimination of preeclampsia and non-preeclampsia, the performance of logistic regression, random forest, and gradient boosting method (GBM) is superior to that of linear discriminant analysis (LDA), neural network, and support vector machine (SVM).
- Biomarkers/Gene Expression Products
- The term “biomarker” refers to a measurable indicator of some biological state or condition. In some instances, a biomarker can be a substance found in a subject, a quantity of the substance, or some other indicator. For example, a biomarker can be the amount of a protein and/or other gene expression products in a sample. In some embodiments, a biomarker is a full-length, unmodified protein. In other embodiments, a biomarker is an alternatively spliced, post-translationally cleaved, or post-translationally chemically modified (e.g., methylated, phosphorylated, glycosylated, formylated, etc) protein. In some embodiments, a biomarker is a complex of a protein with another protein at a particular stoichiometry. In some embodiments, a biomarker is a free protein in the absence of other associated binding partners.
- The methods, compositions and systems as described here also relate to the use of biomarker panels and/or gene expression products for purposes of identification, diagnosis, classification, treatment or to otherwise characterize various conditions of pregnant patients comprising NonPreE, PreE, chronic hypertension, gestational hypertension, or HELLP syndrome. Sets of biomarkers and/or gene expression products useful for classifying biological samples are provided, as well as methods of obtaining such sets of biomarkers. Often, the pattern of levels of gene expression biomarkers in a panel (also known as a signature) is determined from one or more references samples and then used to evaluate the signature of the same panel of biomarkers in a test sample, such as by a measure of similarity between the test sample signature and the reference sample signature.
- The methods provided herein can comprise identifying or ruling out a condition from a combination of a species of PlGF with secondary biomarkers. The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) one or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) one or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- Without wishing to be limited by theory, preeclampsia is thought to originate in abnormal trophoblast invasion, which results in incomplete spiral artery remodeling and hypoperfusion of the placenta, and that this hypoperfusion of the placenta triggers dysfunction in multiple body systems causing the signs and symptoms of preeclampsia. Such dysfunctional systems can include, as a result of placental hypoperfusion, angiogenesis and endothelial function, as evidenced e.g. by imbalances in pro-and-anti-angiogenic factors, many of which are released by the placenta in response to the abnormal physiology of preeclampsia, and which disrupt vascular homeostasis in the mother's body. SFLT1 (Soluble FMS-
like tyrosine kinase 1, a tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PlGF and decreases towards term, and plays an essential role in the development of embryonic vasculature), PlGF (Placental growth factor, a proangiogenic protein peaking at 30 weeks of gestation that stimulates endothelial cell growth, proliferation, and migration), DCN (Decorin, which is a functional component of the extracellular matrix and plays a role in tissue repair and regulation of cell adhesion and migration by binding to ECM molecules), ENG (Endoglin, which in its soluble form, sENG (Endoglin) is a powerful antiangiogenic molecule, and acts by inhibiting TGF-â1 binding), and FGF21 (Fibroblast growth factor 21, which has been demonstrated to be expressed in placental syncytiotrophoblasts, and is both an adipokine and a regulator of glucose transport) are considered to be markers of angiogenesis dysfunction in preeclampsia. Another such dysfunctional system is oxygen signaling, which results from hypoperfusion of the placenta, and leads to upregulation of oxidative stress factors. KIM-1 (Kidney Injury Molecule-1) is considered to be a marker of dysfunction in oxygen signaling in preeclampsia, as its expression is known to increase in response to local hypoxia/ischemia in proximal renal tubule cells. - The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1 or Endoglin; and (b) PlGF (free, dissociated, or % free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1 or Endoglin; (b) PlGF (free, dissociated, or % free); and (c) KIM1. The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1 or Endoglin; (b) PlGF (free, dissociated, or % free); (c) KIM1; and (d) FGF21. The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1 or Endoglin; (b) PlGF (free, dissociated, or % free); (c) KIM1; and (d) Decorin. The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1 or Endoglin; (b) PlGF (free, dissociated, or % free); (c) KIM1; (d) FGF21; and (e) Decorin.
- Additionally, because sFLT1 and Endoglin are highly correlated but each of them also provide additional (exclusive) information that results in improved performance, the biomarkers may comprise both sFLT1 and Endoglin. The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1; (b) Endoglin; and (c) PlGF (free, dissociated, or % free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1; (b) Endoglin; (c) PlGF (free, dissociated, or % free); and (d) KIM1. The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1; (b) Endoglin; (c) PlGF (free, dissociated, or % free); (d) KIM1; and (e) FGF21. The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1; (b) Endoglin; (c) PlGF (free, dissociated, or % free); (d) KIM1; and (e) Decorin. The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) biomarkers comprising: (a) sFLT1; (b) Endoglin; (c) PlGF (free, dissociated, or % free); (d) KIM1; (e) FGF21; and (f) Decorin.
- The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) two or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) three or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) four or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) five or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) six or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) seven or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) all of sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) two biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) three biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) four biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) five biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) six biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) seven biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) no more than two biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) no more than three biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) no more than four biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) no more than five biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) no more than six biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). The methods provided herein can comprise identifying or ruling out a condition (e.g. preeclampsia) from (e.g., based on analysis from) no more than seven biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- Clinical/Therapeutic Applications
- The methods, compositions, systems and kits provided herein can be used to detect, diagnose, predict or monitor a condition of a pregnant patient. In some instances, the methods, compositions, systems and kits described herein provide information to a medical practitioner that can be useful in making a clinical therapeutic decision. Clinical and therapeutic decisions can include decisions to: continue with a particular therapy, modify a particular therapy, alter the dosage of a particular therapy, stop or terminate a particular therapy, altering the frequency of a therapy, introduce a new therapy, introduce a new therapy to be used in combination with a current therapy, or any combination of the above. In some instances, medical action taken may comprise watchful waiting or the administration of one or more additional diagnostic tests of the same or different nature. In some cases, a clinical decision may be made to not induce labor, or to proceed with ambulant monitoring of the subject. In some cases, the methods provided herein can be applied in an experimental setting, e.g., clinical trial. In some instances, the methods provided herein can be used to monitor a pregnant patient who is being treated with an experimental agent such as an angiogenic/antiangiogenic drug, compound, or therapeutic cell type. In some instances, the methods provided herein can be useful to determine whether a subject can be administered an experimental agent (e.g., an agonist, antagonist, peptidomimetic, protein, peptide, nucleic acid, therapeutic cell, small molecule, or other drug candidate) to reduce the risk of preeclampsia. Thus, the methods described herein can be useful in determining if a subject can be effectively treated with an experimental agent and for monitoring the subject for risk of preeclampsia.
- Detecting/Diagnosing a Condition of a Pregnant Patient
- The methods, compositions, systems and kits provided herein are particularly useful for detecting, diagnosing, or ruling out a condition of a pregnant patient such as a condition the pregnant patient has at the time of testing. An exemplary condition that can be detected, diagnosed, or ruled out with the present method includes preeclampsia. The methods, compositions, systems, and kits provided herein can also be useful, in combination with other standard clinical data collected for pregnant patients, for ruling in or ruling out a diagnosis of preeclampsia, hypertension, gestational hypertension, or HELLP syndrome. The methods provided herein are particularly useful for pregnant patients who have exhibited one or more new-onset symptoms associated with preeclampsia prior to testing (e.g., hypertension, proteinuria, low platelet count, elevated serum creatinine levels, elevated liver enzymes, pulmonary edema, or cerebral/visual symptoms), such that the patients are suspected of having preeclampsia.
- In some embodiments, the methods, compositions, systems and kits provided herein can rule out a diagnosis of preeclampsia for a specified number of days in the future. In some instances, the specified number of days in the future is 1 to 30 days. In some instances, the specified number of days in the future is at least 1 day. In some instances, the specified number of days in the future is at most 30 days. In some instances, the specified number of days in the future is 1 day to 5 days, 1 day to 10 days, 1 day to 30 days, 5 days to 10 days, 5 days to 30 days, or 10 days to 30 days. In some instances, the specified number of days in the future is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 days. In some preferred embodiments, the specified number of days in the future is 5 days to 10 days. In other preferred embodiments, the specified number of days in the future is 5, 6, 7, 8, 9, or 10 days. In some embodiments, the methods, compositions, systems and kits provided herein can rule-out mothers for hospital admission and preterm delivery. In some embodiments, the methods, compositions, systems and kits provided herein can rule out a diagnosis of preeclampsia for a specified number of weeks in the future. In some instances, the specified number of weeks is at least 1 week. In some instances, the specified number of weeks is at least 2 weeks. In some instances, the specified number of weeks is at least 3 weeks. In some instances, the specified number of weeks is at least 4 weeks. In some instances, the specified number of weeks is at least 5 weeks. In some instances, the specified number of weeks is at least 6 weeks. In some instances, the specified number of weeks is at most 1 week. In some instances, the specified number of weeks is at most 2 weeks. In some instances, the specified number of weeks is at most 3 weeks. In some instances, the specified number of weeks is at most 4 weeks. In some instances, the specified number of weeks is at most 5 weeks. In some instances, the specified number of weeks is at most 6 weeks.
- In some embodiments, the methods, compositions, systems and kits provided herein can rule out a diagnosis of preeclampsia for a specified number of weeks in the future with a particular PPV. In some cases, the positive predictive value (PPV) is at least about 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, or 57%, or any range in between these values.
- In some embodiments, the methods, compositions, systems and kits provided herein can rule out a diagnosis of preeclampsia for a specified number of weeks in the future with a particular NPV. The NPV can be at least about 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 95.2%, 95.5%, 95.7%, 96%, 96.2%, 96.5%, 96.7%, 97%, 97.2%, 97.5%, 97.7%, 98%, 98.2%, 98.5%, 98.7%, 99%, 99.2%, 99.5%, 99.7%, or 99.9%, or any range in between these values.
- In some embodiments, the methods, compositions, systems and kits provided herein can rule out a diagnosis of preeclampsia for a specified number of weeks in the future with a particular AUC. In some cases, the AUC of can be at least about 50%, 53%, 55%, 57%, 60%, 63%, 65%, 67%, 70%, 72%, 75%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, or any range in between these values.
- In some embodiments, the methods, compositions, systems and kits provided herein can rule out a diagnosis of preeclampsia for a specified number of weeks in the future with a particular AUP. The AUP can be at least about 50%, 53%, 55%, 57%, 60%, 63%, 65%, 67%, 70%, 72%, 75%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, or any range in between these values.
- The diagnosis, detection, or ruling out of a condition of the pregnant patient can be particularly useful in limiting the number of unnecessary invasive medical interventions that are administered to the patient, and/or indicating alternative less-invasive therapeutic interventions such as pharmacological therapies (anticonvulsants, antihypertensives, central alpha agonists, alpha-blockers, beta-blockers, calcium-channel blockers, vasodilators, cyclooxygenase inhibitors, or, acetylsalicylic acid). For example, the methods provided herein can limit, delay, or eliminate the use of preterm cesarean delivery or labor induction in patients suspected of having preeclampsia via high-confidence ruling out of a diagnosis of preeclampsia in the pregnant patient (e.g., via a high negative predictive value of the methods, compositions, systems, and kits provided herein).
- In some cases, the methods provided herein can inform the clinical decision to proceed to delivery (e.g. when the pregnant patient is identified as being at-risk of preeclampsia). Such a decision may involve the administration of a labor-inducing or cervical ripening agent (e.g. prostaglandin, oxytocin, misoprostol, mifepristone, or relaxin), a corticosteroid (to aid in lung development prior to delivery), and/or performing a C-section.
- In a further embodiment, the methods, compositions, systems and kits provided herein can be used alone or in combination with other standard diagnosis methods currently used to detect, diagnose, or rule out a condition of a pregnant patient, such as but not limited to blood pressure measurement, urine protein measurement, blood platelet counting, serum creatinine level measurement, creatinine clearance measurement, urine protein/creatinine ratio measurement, serum transaminase level measurement, serum LDH level measurement, serum bilirubin level measurement, or Doppler ultrasound indices (e.g., uterine artery indices). For example, hypertension in a pregnant patient can be indicative of conditions such as chronic hypertension, gestational hypertension, or preeclampsia; ruling out the diagnosis of preeclampsia via the methods, compositions, systems and kits provided herein allows for the patient to be correctly diagnosed with chronic hypertension or gestational hypertension.
- Predicting a Condition of a Pregnant Patient
- In some embodiments, the methods provided herein can predict preeclampsia prior to actual onset of the condition or symptoms associated with the condition (e.g., hypertension or proteinuria). In some instances, the methods provided herein can predict preeclampsia or other disorders in a pregnant patient at least 1 day, 1 week, 2 weeks, 3 weeks, 1 month, 2 months prior to onset of the condition or symptoms associated with the condition. In other instances, the methods provided herein can predict preeclampsia or other disorders in a pregnant patient at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 or 31 days prior to onset. In other instances, the methods provided herein can predict preeclampsia or other disorders in a pregnant patient at least 1, 2, 3, or 4 months prior to onset.
- Monitoring a Condition of a Pregnant Patient
- Provided herein are methods, systems, kits and compositions for monitoring a condition of a pregnant patient. Often, the monitoring is conducted by serial testing, such as serial non-invasive tests, serial minimally-invasive tests (e.g., blood draws), or some combination thereof. Preferably, the monitoring is conducted by administering serial non-invasive tests or serial minimally-invasive tests (e.g., blood draws).
- In some instances, the pregnant patient is monitored as needed (e.g., on an as-needed basis) using the methods described herein. Additionally, or alternatively the pregnant patient can be monitored weekly, monthly, or at any pre-specified intervals. In some instances, the pregnant patient is monitored at least once every 24 hours. In some instances, the pregnant patient is monitored at least once every 1 day to 30 days. In some instances, the pregnant patient is monitored at least once every at least 1 day. In some instances, the pregnant patient is monitored at least once every at most 30 days. In some instances the pregnant patient is monitored at least (optionally on average) once every 1 day to 5 days, 1 day to 10 days, 1 day to 15 days, 1 day to 20 days, 1 day to 25 days, 1 day to 30 days, 5 days to 10 days, 5 days to 15 days, 5 days to 20 days, 5 days to 25 days, 5 days to 30 days, 10 days to 15 days, 10 days to 20 days, 10 days to 25 days, 10 days to 30 days, 15 days to 20 days, 15 days to 25 days, 15 days to 30 days, 20 days to 25 days, 20 days to 30 days, or 25 days to 30 days. In some instances, the pregnant patient is monitored at least once every 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 28, 29, 30 or 31 days. In some instances, the pregnant patient is monitored at least once every 1, 2, or 3 months. In some instances, the pregnant patient is monitored via the methods described herein no more frequently than one week, 10 days, two weeks, three weeks, or one month. In other words, the predictive value of the some of the methods described herein can be of clinical use for at least one week, at least 10 days, at least two weeks, at least three weeks, or at least one month.
- In some instances, biomarker expression levels in the patients can be measured, for example, within, one week, two weeks, three weeks, or four weeks after detection of one or more symptoms associated with preeclampsia (e.g., hypertension or proteinuria). In some methods, biomarker expression levels are determined at regular intervals, e.g., every 1 week, 2 weeks, 3 weeks, 1 month, 2 months or 3 months post-conception, after the beginning of the 2nd trimester, after the beginning of the 3rd trimester, or after
week 20 of the pregnancy, either indefinitely, or until evidence of a condition is observed. In some methods, biomarker expression levels are determined at regular intervals afterweek - Therapeutic Decisions/Regimens
- The results of diagnosing, predicting, ruling out, or monitoring a condition of the pregnant patient can be useful for informing a clinical or therapeutic decision such as determining or monitoring a therapeutic regimen.
- In some embodiments, an entity that acquires sample data and/or classifies a sample from a patient as having preeclampsia is other than the physician, caregiver, or medical institution performing the treatment. In some embodiments, the entity acquiring sample data (e.g. levels of levels of one or more biomarkers described herein), calculating an index based (at least in part) on the levels of the plurality of the protein biomarkers, and/or determining risk of preeclampsia is a third-party testing service. Thus, in some embodiments, determining or monitoring a therapeutic regimen first comprises receiving information from a third-party testing service, which can comprise, for example (but not limited to), classification of a sample as being at risk or not of preeclampsia, risk of a pregnant patient having preeclampsia, levels of a plurality of protein biomarkers from the sample associated with preeclampsia (e e.g. levels of levels of one or more biomarkers described herein), or the likelihood a pregnant patient will deliver preterm.
- In some embodiments, an entity that acquires sample data, determines the risk of preterm birth of the patient from the sample, and/or classifies a sample from a patient as having a significant risk of preterm birth is the same entity that performing the treatment.
- In some instances, determining a therapeutic regimen can comprise administering a therapeutic drug. In some instances, determining a therapeutic regimen comprises modifying, continuing, initiating or stopping a therapeutic regimen. In some instances, determining a therapeutic regimen comprises treating the disease or condition (e.g., preeclampsia, eclampsia, gestational hypertension, hypertension, or HELLP syndrome). In some instances, the therapy is an anti-hypertensive therapy. In some instances, the therapy is an anti-cyclooxygenase (COX) therapy. In some instances, the therapy is an anti-convulsant therapy.
- Modifying the therapeutic regimen can comprise terminating a therapy. Modifying the therapeutic regimen can comprise altering a dosage of a therapy. Modifying the therapeutic regimen can comprise altering a frequency of a therapy. Modifying the therapeutic regimen can comprise administering a different therapy. In some instances, the results of diagnosing, predicting, or monitoring a condition of the pregnant patient can be useful for informing a therapeutic decision such as caesarean delivery. Other examples of therapeutic decisions can be cervical ripening and/or labor induction. Examples of agents that can be used for cervical ripening and/or labor induction include prostaglandins, misoprostol, mifepristone, relaxin, and oxytocin. Other examples of therapeutic decisions can be cesarean delivery.
- Examples of a therapeutic regimen can include administering compounds or agents having anti-hypertensive properties (e.g., central alpha agonists such as methyldopa, vasodilators such as clonidine, diazoxide, hydralazine and prazosin, calcium-channel blockers such as nifedipine and verapamil, alpha-blockers such as labetalol, or beta-blockers such as oxprenolol), compounds or agents having anti-cyclooxygenase activity (e.g., acetylsalicylic acid), or compounds having anti-convulsant activity (e.g., phenytoin or magnesium sulfate). These compounds can be used alone or in combination.
- In some cases, modifying the therapeutic regimen can comprise proceeding with treatment of said pregnant human in a manner that avoids unnecessary treatment of preeclampsia. For instance, in some embodiments, managing the pregnant human subject identified not as at risk for preeclampsia comprises ambulant monitoring, or refraining from the administration of any drug for treating preeclampsia. In some instances, in patients that are identified as patients that should not be treated for preeclampsia, antihypertensive drugs (rather than delivery) may be prescribed and/or administered to the patient. In some instances, in patients that are identified as not patients that should not be treated for preeclampsia, the preeclampsia test is repeated at an increased frequency. The test can be re-performed, and sample re-drawn from the patient weekly, monthly, or at any pre-specified intervals. In some instances, the pregnant patient is monitored at least once every 24 hours. In some instances, the pregnant patient is monitored at least once every 1 day to 30 days. In some instances, the pregnant patient is monitored at least once every at least 1 day. In some instances, the pregnant patient is monitored at least once every at most 30 days. In some instances the pregnant patient is monitored at least (optionally on average) once every 1 day to 5 days, 1 day to 10 days, 1 day to 15 days, 1 day to 20 days, 1 day to 25 days, 1 day to 30 days, 5 days to 10 days, 5 days to 15 days, 5 days to 20 days, 5 days to 25 days, 5 days to 30 days, 10 days to 15 days, 10 days to 20 days, 10 days to 25 days, 10 days to 30 days, 15 days to 20 days, 15 days to 25 days, 15 days to 30 days, 20 days to 25 days, 20 days to 30 days, or 25 days to 30 days. In some instances, the pregnant patient is monitored at least once every 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 28, 29, 30 or 31 days. In some instances, the pregnant patient is monitored at least once every 1, 2, or 3 months. In some instances, the pregnant patient is monitored via the methods described herein no more frequently than one week, 10 days, two weeks, three weeks, or one month. In other words, the predictive value of the some of the methods described herein can be of clinical use for at least one week, at least 10 days, at least two weeks, at least three weeks, or at least one month.
- Sensitivity, Specificity, NPV, PPV, AUC, AUP, and Accuracy
- The methods, kits, and systems disclosed herein for use in identifying, classifying (or ruling out a classification) or characterizing a sample can be characterized by having a specificity of at least about 80% using the methods disclosed herein. In some embodiments, the specificity of the methods is at least about 85%. In some embodiments, the specificity of the methods is at least about 90%. In some embodiments, the specificity of the methods is at least about 95%. The specificity of the method can be at least about 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%, or any range in between these values.
- In some embodiments, the present invention provides a method of identifying, classifying (or ruling out a classification) or characterizing a sample that gives a sensitivity of at least about 80% using the methods disclosed herein. In some embodiments, the sensitivity of the methods is at least 85%. In some embodiments, the sensitivity of the methods is at least 90%. In some embodiments, the sensitivity of the methods is at least 95%. The sensitivity of the method can be at least about 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%, or any range in between these values.
- The methods, kits and systems disclosed herein can improve upon the accuracy of current methods of monitoring or predicting a status or outcome of a pregnancy (e.g. preeclampsia) or identifying or ruling out a classification of a sample. The accuracy of the methods, kits, and systems disclosed herein can be at least about 50%, 53%, 55%, 57%, 60%, 63%, 65%, 67%, 70%, 72%, 75%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, or any range in between these values.
- The methods, kits, and systems for use in identifying, classifying (or ruling out a classification) or characterizing a sample can be characterized by having a negative predictive value (NPV) greater than or equal to 90%. The NPV can be at least about 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 95.2%, 95.5%, 95.7%, 96%, 96.2%, 96.5%, 96.7%, 97%, 97.2%, 97.5%, 97.7%, 98%, 98.2%, 98.5%, 98.7%, 99%, 99.2%, 99.5%, 99.7%, or 99.9%, or any range in between these values. The NPV can be greater than or equal to 95%. The NPV can be greater than or equal to 96%. The NPV can be greater than or equal to 97%. The NPV can be greater than or equal to 98%.
- The methods, kits, and/or systems disclosed herein for use in identifying, classifying (or ruling out a classification) or characterizing a sample (e.g. for preeclampsia) can be characterized by having a positive predictive value (PPV) of at least about 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, or 57%, or any range in between these values using the methods disclosed herein.
- The methods, kits and systems disclosed herein can improve upon the AUC of current methods of monitoring or predicting a status or outcome of a pregnancy (e.g. preeclampsia) or identifying or ruling out a classification of a sample. The AUC of the methods, kits, and systems disclosed herein can be at least about 50%, 53%, 55%, 57%, 60%, 63%, 65%, 67%, 70%, 72%, 75%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, or any range in between these values.
- The methods, kits and systems disclosed herein can improve upon the AUP of current methods of monitoring or predicting a status or outcome of a pregnancy (e.g. preeclampsia) or identifying or ruling out a classification of a sample. The AUP of the methods, kits, and systems disclosed herein can be at least about 50%, 53%, 55%, 57%, 60%, 63%, 65%, 67%, 70%, 72%, 75%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.1%, 99.2%, 99.3%, 99.4%, 99.5%, 99.6%, 99.7%, 99.8%, 99.9%, or any range in between these values.
- The methods, kits, and systems disclosed herein for use in diagnosing, prognosing, and/or monitoring a status or outcome of a pregnancy in a subject in need thereof can be characterized by having an accuracy of at least about 80%, 82%, 85%, 87%, 90%, 92%, 95%, or 97% or any range in between these values.
- The methods, kits, and systems disclosed herein for use in diagnosing, prognosing, and/or monitoring a status or outcome of a pregnancy in a subject in need thereof can be characterized by having a specificity of at least about 80%, 82%, 85%, 87%, 90%, 92%, 95%, or 97%, or any range in between these values.
- The methods, kits, and systems disclosed herein for use in diagnosing, prognosing, and/or monitoring a status or outcome of a pregnancy in a subject in need thereof can be characterized by having a sensitivity of at least about 80%, 82%, 85%, 87%, 90%, 92%, 95%, or 97%, or any range in between these values.
- The methods, kits, and systems disclosed herein for use in diagnosing, prognosing, and/or monitoring a status or outcome of a pregnancy in a subject in need thereof can be characterized by having a negative predictive value (NPV) greater than or equal to 90%. The NPV can be at least about 90%, 91%, 92%, 93%, 94%, 95%, 95.2%, 95.5%, 95.7%, 96%, 96.2%, 96.5%, 96.7%, 97%, 97.2%, 97.5%, 97.7%, 98%, 98.2%, 98.5%, 98.7%, 99%, 99.2%, 99.5%, 99.7%, or 99.9%, or any range in between these values. The NPV can be greater than or equal to 95%. The NPV can be greater than or equal to 96%. The NPV can be greater than or equal to 97%. The NPV can be greater than or equal to 98%.
- The methods, kits, and systems disclosed herein for use in diagnosing, prognosing, and/or monitoring a status or outcome of a pregnancy in a subject in need thereof can be characterized by having a positive predictive value (PPV) of at least about 80%. In some embodiments, the methods, kits, and systems disclosed herein for use in diagnosing, prognosing, and/or monitoring a status or outcome of a pregnancy in a subject in need thereof can be characterized by having a positive predictive value (PPV) of at least about 85%. In some embodiments, the methods, kits, and systems disclosed herein for use in diagnosing, prognosing, and/or monitoring a status or outcome of a pregnancy in a subject in need thereof can be characterized by having a positive predictive value (PPV) of at least about 90%. The PPV can be at least about 80%, 85%, 90%, 95%, 95.2%, 95.5%, 95.7%, 96%, 96.2%, 96.5%, 96.7%, 97%, 97.2%, 97.5%, 97.7%, 98%, 98.2%, 98.5%, 98.7%, 99%, 99.2%, 99.5%, 99.7%, or 99.9%, or any range in between these values. The PPV can be greater than or equal to 95%. The PPV can be greater than or equal to 98%.
- In some embodiments, disclosure provides a test for confirming preeclampsia in a subject, preferably a pregnant subject, wherein the test is able to discern subjects not having preeclampsia but having one or more symptoms associated with preeclampsia from subjects having by preeclampsia with an NPV of at least about 90%, 91%, 92%, 93%, 94%, 95%, 95.2%, 95.5%, 95.7%, 96%, 96.2%, 96.5%, 96.7%, 97%, 97.2%, 97.5%, 97.7%, 98%, 98.2%, 98.5%, 98.7%, 99%, 99.2%, 99.5%, 99.7%, or 99.9%, or any range in between these values. The one or more symptoms associated with preeclampsia can be diabetes (e.g. gestational, type I or type II), higher than normal glucose level, hypertension (e.g. chronic or non-chronic), excessive or sudden weight gain, higher than normal weight, obesity, higher than normal body mass index (BMI), abnormal weight gain, abnormal blood pressure, water retention, hereditary factors, abnormal proteinuria, headache, edema, abnormal protein/creatinine ratio, abnormal platelet count, stress, nulliparity, abnormal Papanicolaou test results (Pap smear), prior preeclampsia episodes (e.g., personal history of PreE), familial history of preeclampsia, preeclampsia in prior pregnancies, renal disease, thrombophilia, or any combination thereof. Gestational age may also be used in tests, such as tests for ruling out preeclampsia.
- Computer Program
- The methods, kits, and systems disclosed herein can include at least one computer program or use of the same. A computer program can include a sequence of instructions, executable in the digital processing device's CPU (i.e. processor), written to perform a specified task. Computer readable instructions can be implemented as program modules, such as functions, objects, Application Programming Interfaces (APIs), data structures, and the like, that perform particular tasks or implement particular abstract data types. In light of the disclosure provided herein, those of skill in the art will recognize that a computer program can be written in various versions of various languages.
- The functionality of the computer readable instructions can be combined or distributed as desired in various environments. The computer program will normally provide a sequence of instructions from one location or a plurality of locations.
- Further disclosed herein are systems for classifying (or ruling out a classification) one or more samples and uses thereof. The system can comprise (a) a digital processing device comprising an operating system configured to perform executable instructions and a memory device; (b) a computer program including instructions executable by the digital processing device to classify a sample from a subject comprising: (i) a first software module configured to receive a biomarker expression profile of one or more biomarkers from the sample from the subject; (ii) a second software module configured to analyze the biomarker expression profile from the subject; and (iii) a third software module configured to classify the sample from the subject based on a classification system. In some embodiments, the classification system comprises two classes. In other embodiments, the classification system comprises two or more classes. At least two of the classes can be selected from preeclampsia, non-preeclampsia (e.g., for at least a period of time), normal pregnancy, complicated pregnancy, and gestational hypertension. Analyzing the biomarker expression profile from the subject can comprise applying an algorithm. Analyzing the biomarker expression profile can comprise normalizing the biomarker expression profile from the subject.
-
FIG. 11 shows a computer system (also “system” herein) 401 programmed or otherwise configured for implementing the methods of the disclosure, such as producing a selector set and/or for data analysis. Thesystem 401 includes a central processing unit (CPU, also “processor” and “computer processor” herein) 405, which can be a single core or multi core processor, or a plurality of processors for parallel processing. Thesystem 401 also includes memory 410 (e.g., random-access memory, read-only memory, flash memory), electronic storage unit 415 (e.g., hard disk), communications interface 420 (e.g., network adapter) for communicating with one or more other systems, andperipheral devices 425, such as cache, other memory, data storage and/or electronic display adapters. Thememory 410,storage unit 415,interface 420 andperipheral devices 425 are in communication with theCPU 405 through a communications bus (solid lines), such as a motherboard. Thestorage unit 415 can be a data storage unit (or data repository) for storing data. Thesystem 401 is operatively coupled to a computer network (“network”) 430 with the aid of thecommunications interface 420. Thenetwork 430 can be the Internet, an internet and/or extranet, or an intranet and/or extranet that is in communication with the Internet. Thenetwork 430 in some instances is a telecommunication and/or data network. Thenetwork 430 can include one or more computer servers, which can enable distributed computing, such as cloud computing. Thenetwork 430 in some instances, with the aid of thesystem 401, can implement a peer-to-peer network, which can enable devices coupled to thesystem 401 to behave as a client or a server. - The
system 401 is in communication with aprocessing system 435. Theprocessing system 435 can be configured to implement the methods disclosed herein. In some examples, theprocessing system 435 is a microfluidic qPCR system. In other examples, theprocessing system 435 is an ALPHA screen or other plate reader. In other examples, theprocessing system 435 is a FACS sorter or analyzer. Theprocessing system 435 can be in communication with thesystem 401 through thenetwork 430, or by direct (e.g., wired, wireless) connection. In some embodiments, raw data from the processing system (e.g. a biomarker expression profile) is uploaded through the network to the system for processing (e.g. sample classification or determination of a probability of a certain classification). This data transfer may be direct (e.g. FTP, TCP, or other direct network connection between theprocessing system 435 and the system 401), or indirect (e.g. transfer to a cloud storage system which can be accessed by the system 401). - Methods as described herein can be implemented by way of machine (or computer processor) executable code (or software) stored on an electronic storage location of the
system 401, such as, for example, on thememory 410 orelectronic storage unit 415. During use, the code can be executed by theprocessor 405. In some examples, the code can be retrieved from thestorage unit 415 and stored on thememory 410 for ready access by theprocessor 405. In some situations, theelectronic storage unit 415 can be precluded, and machine-executable instructions are stored onmemory 410. - Digital Processing Device
- The methods, kits, and systems disclosed herein can include a digital processing device or use of the same. In further embodiments, the digital processing device includes one or more hardware central processing units (CPU) that carry out the device's functions. In still further embodiments, the digital processing device further comprises an operating system configured to perform executable instructions. In some embodiments, the digital processing device is optionally connected a computer network. In further embodiments, the digital processing device is optionally connected to the Internet such that it accesses the World Wide Web. In still further embodiments, the digital processing device is optionally connected to a cloud computing infrastructure. In other embodiments, the digital processing device is optionally connected to an intranet. In other embodiments, the digital processing device is optionally connected to a data storage device.
- In accordance with the description herein, suitable digital processing devices include, by way of non-limiting examples, server computers, desktop computers, laptop computers, notebook computers, sub-notebook computers, netbook computers, netpad computers, set-top computers, handheld computers, Internet appliances, mobile smartphones, tablet computers, personal digital assistants, video game consoles, and vehicles. Those of skill in the art will recognize that many smartphones are suitable for use in the system described herein. Those of skill in the art will also recognize that select televisions, video players, and digital music players with optional computer network connectivity are suitable for use in the system described herein. Suitable tablet computers include those with booklet, slate, and convertible configurations, known to those of skill in the art.
- The digital processing device will normally include an operating system configured to perform executable instructions. The operating system is, for example, software, including programs and data, which manages the device's hardware and provides services for execution of applications. Those of skill in the art will recognize that suitable server operating systems include, by way of non-limiting examples, FreeBSD, OpenBSD, NetBSD®, Linux, Apple® Mac OS X Server®, Oracle® Solaris®, Windows Server®, and Novell® NetWare®. Those of skill in the art will recognize that suitable personal computer operating systems include, by way of non-limiting examples, Microsoft® Windows®, Apple® Mac OS X®, UNIX®, and UNIX-like operating systems such as GNU/Linux®. In some embodiments, the operating system is provided by cloud computing. Those of skill in the art will also recognize that suitable mobile smart phone operating systems include, by way of non-limiting examples, Nokia® Symbian® OS, Apple® iOS®, Research In Motion® BlackBerry OS®, Google® Android®, Microsoft® Windows Phone® OS, Microsoft® Windows Mobile® OS, Linux®, and Palm® WebOS®.
- The device generally includes a storage and/or memory device. The storage and/or memory device is one or more physical apparatuses used to store data or programs on a temporary or permanent basis. In some embodiments, the device is volatile memory and requires power to maintain stored information. In some embodiments, the device is non-volatile memory and retains stored information when the digital processing device is not powered. In further embodiments, the non-volatile memory comprises flash memory. In some embodiments, the non-volatile memory comprises dynamic random-access memory (DRAM). In some embodiments, the non-volatile memory comprises ferroelectric random-access memory (FRAM). In some embodiments, the non-volatile memory comprises phase-change random access memory (PRAM). In other embodiments, the device is a storage device including, by way of non-limiting examples, CD-ROMs, DVDs, flash memory devices, magnetic disk drives, magnetic tapes drives, optical disk drives, and cloud computing-based storage. In further embodiments, the storage and/or memory device is a combination of devices such as those disclosed herein.
- A display to send visual information to a user will normally be initialized. Examples of displays include a cathode ray tube (CRT, a liquid crystal display (LCD), a thin film transistor liquid crystal display (TFT-LCD, an organic light emitting diode (OLED) display. In various further embodiments, on OLED display is a passive-matrix OLED (PMOLED) or active-matrix OLED (AMOLED) display. In some embodiments, the display can be a plasma display, a video projector or a combination of devices such as those disclosed herein.
- The digital processing device would normally include an input device to receive information from a user. The input device can be, for example, a keyboard, a pointing device including, by way of non-limiting examples, a mouse, trackball, track pad, joystick, game controller, or stylus; a touch screen, or a multi-touch screen, a microphone to capture voice or other sound input, a video camera to capture motion or visual input or a combination of devices such as those disclosed herein.
- Non-Transitory Computer Readable Storage Medium
- The methods, kits, and systems disclosed herein can include one or more non-transitory computer readable storage media encoded with a program including instructions executable by the operating system to perform and analyze the test described herein; preferably connected to a networked digital processing device. The computer readable storage medium is a tangible component of a digital device that is optionally removable from the digital processing device. The computer readable storage medium includes, by way of non-limiting examples, CD-ROMs, DVDs, flash memory devices, solid state memory, magnetic disk drives, magnetic tape drives, optical disk drives, cloud computing systems and services, and the like. In some instances, the program and instructions are permanently, substantially permanently, semi-permanently, or non-transitorily encoded on the media.
- A non-transitory computer-readable storage media can be encoded with a computer program including instructions executable by a processor to create or use a classification system. The storage media can comprise (a) a database, in a computer memory, of one or more clinical features of two or more control samples, wherein (i) the two or more control samples can be from two or more subjects; and (ii) the two or more control samples can be differentially classified based on a classification system comprising two or more classes; (b) a first software module configured to compare the one or more clinical features of the two or more control samples; and (c) a second software module configured to produce a classifier set based on the comparison of the one or more clinical features. At least two of the classes can be selected from preeclampsia, non-preeclampsia, normal pregnancy, complicated pregnancy, and gestational hypertension.
- Antigen/Biomarker Detection (E.g., Antibodies)
- In some embodiments, at least one antigen binding reagent is used to detect any of the biomarkers identified herein. In some embodiments, the antigen binding reagent can be an antibody (monoclonal or polyclonal), antigen-binding fragment (e.g. Fab, Fab′, F(ab)2, F(abc)2, or Fv fragment) of an antibody, or an antibody derivative (e.g. diabody, linear antibody, or scFv).
- In some aspects, the present disclosure provides for a method of determining whether a pregnant human female is at risk of suffering from preeclampsia comprising: determining a free Placental Growth Factor to dissociated Placental Growth Factor (PlGF-f:PlGF-d) ratio of a biological sample collected from the pregnant human female. In some embodiments, the pregnant human female is identified as having a heightened risk of suffering from preeclampsia when a PlGF-f:PlGF-d ratio less than a first threshold value is determined. In some embodiments, the pregnant human female is identified as having a reduced risk of suffering from preeclampsia when a PlGF-f:PlGF-d ratio greater than a second threshold value is determined. In some embodiments, a PlGF-f:PlGF-d ratio less than a first threshold value indicates a heightened risk that the pregnant human female will suffer from preeclampsia. In some embodiments, a PlGF-f:PlGF-d ratio greater than a second threshold value indicates a reduced risk that the pregnant human female will suffer from preeclampsia.
- The first and/or second threshold value can be determined by supervised learning using a trained algorithm. The trained algorithm can be trained using e.g. supervised learning approaches described herein.
- In some embodiments, the method comprises collecting the biological sample. The biological sample may be collected by any of the methods described herein. The biological sample may be collected by venipuncture, fingerstick sampling, arterial sampling, or cervicovaginal fluid collection using an ophthalmic sponge. The biological sample may be any of the samples described for analysis herein. The biological sample may be a fluid, whole blood, peripheral blood, serum, plasma, urine, cervicovaginal secretion, or amniotic fluid sample.
- A variety of different treatments may be applied to dissociate a PlGF complex. In some embodiments, the treatment is an elevated temperature; e.g. least 30° C., at least 37° C., or at least 45° C. In some embodiments, the treatment comprises increasing or decreasing the pH (e.g. to less than 6 or greater than 8). In some embodiments, the treatment is a surfactant, such as an anionic surfactant, a cationic surfactant, a zwitterionic surfactant, a nonionic surfactant, SDS, PFOA, cetrimonium bromide (CTAB), cetylpyridinium chloride (CPC), benzalkonium chloride (BAC), benzethonium chloride (BZT), dimethyldioctadecylammonium chloride, dioctadecyldimethylammonium bromide (DODAB), CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate), cocamidopropyl hydroxysultaine, Triton-X-100, tween-20, tween-40-tween-60, or tween-80, or any combination thereof. In some embodiments, the treatment is a chaotropic agent, such as a low concentration of urea or guanidinium hydrochloride. In some embodiments, the treatment is an agent that adjusts the ionic strength of the solution, such as NaCl, KCl, or ammonium chloride.
- In some embodiments, the treatment is a steric treatment. Steric treatments include antibodies, antibody fragments or derivatives, aptamers, peptides, proteins, or other agents that bind an epitope of free PlGF or free sFLT, PlGF, or sFLT1 and prevent reassembly of a PlGF/sFLT1complex.
- In some embodiments, a PlGF binding or blocking agent is selected based on reference to a published crystal structure of PlGF protein (see e.g. Iyer et al. J Biol Chem. 2001 Apr. 13; 276(15):12153-61. Epub 2000 Nov. 7). In some embodiments, the PlGF binding or blocking agent binds to residues on the PlGF-1 molecule that are involved in recognition of s-Flt-1.
- In some embodiments, an agent, or an apparatus for dissociating PlGF (e.g. PlGF complexes) are used in methods, systems, kits, or compositions described herein. Such agent or apparatus can include a device for application for heat to a sample from a subject (e.g. a multiwell plate in combination with a thermocycler or heat block), a pH adjustment agent (e.g. hydrochloric acid solution or sodium/potassium acetate for acidic adjustment, or bicarbonate, sodium hydroxide, or potassium hydroxide for alkaline adjustment), a surfactant (e.g., anionic surfactant, a cationic surfactant, a zwitterionic surfactant, a nonionic surfactant, SDS, PFOA, cetrimonium bromide (CTAB), cetylpyridinium chloride (CPC), benzalkonium chloride (BAC), benzethonium chloride (BZT), dimethyldioctadecylammonium chloride, dioctadecyldimethylammonium bromide (DODAB), CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate), cocamidopropyl hydroxysultaine, Triton-X-100, tween-20, tween-40-tween-60, or tween-80, or any combination thereof), a chaotropic agent (e.g. urea or guanidinium hydrochloride), an ionic strength adjustment agent (e.g., a concentrated solution of NaCl, KCl, or ammonium chloride), or a steric agent. In some embodiments the agent is provided in the form of a concentrated solution. In some embodiments the concentrated solution consists essentially of the reagent. In some embodiments, the concentrated solution has a concentration of the agent of at least 50 mM, at least 100 mM, at least 150 mM, at least 200 mM, at least 250 mM, at least 300 mM, at least 350 mM, at least 400 mM, at least 450 mM, at least 500 mM, at least 550 mM, at least 600 mM, at least 650 mM, at least 700 mM, at least 750 mM, at least 800 mM, at least 850 mM, at least 900 mM, at least 950 mM, at least 1 M, at least 2 M, at least 4M, or at least 5 M.
- In some embodiments, dissociated PlGF level or total PlGF level is measured by solid-phase extraction rather than dissociation per se. In some iterations of this embodiment, recombinant sFLT1 or another PlGF binding partner is added to the sample at a supersaturating concentration to bind all free PlGF, and the PlGF complex is captured using an sFLT antibody (or an antibody against the other PlGF binding partner). The captured PlGF is then dissociated from the sFLT (or the other PlGF binding partner) and measured using a PlGF ELISA, immunoassay, or mass spectrometry using standard temperature conditions. In other iterations of this embodiment, PlGF(f) is measured by anion exchange chromatography, cation exchange chromatography, or gel filtration chromatography or filtration by identifying a free PlGF peak separate from the other PlGF complexes.
- In some embodiments, following treatment to dissociate a PlGF complex, the PlGF complex is then treated with an agent to prevent reassembly of the PlGF complex with other agents.
- In some embodiments, PlGF-d is not determined by dissociation but rather by binding of an antibody, antibody fragment, antibody derivative, aptamer, peptide binder, or protein binder with an alternative epitope to those described herein. In some cases, such an antibody binds PlGF at an epitope that is not blocked by binding of sFLT.
- Treatments to dissociate a PlGF complex may be applied for various amounts of time. In some embodiments, the treatment is applied for at least 5, 10, 15, 20, 25, 30, 40, 50, 60, 90, or 120 minutes, or at least 8, 12, or 16 hours.
- In some embodiments the first unmodified portion of said biological sample has not been stored at a temperature above about 20° C. In some embodiments, the first unmodified sample is then contacted to a PlGF capture antibody at a temperature of e.g. 30° C., 28° C., 25° C., or 22° C. or less to prevent dissociation any PlGF:sFLT complexes in the sample. In some embodiment, the first unmodified sample is contacted to a PlGF capture antibody with high affinity for a brief period of time to prevent dissociation of any PlGF:sFLT complexes in the sample. In some embodiments, the PlGF is measured in a homogenous assay format (e.g. a format that does not require washing such as TR-FRET, LOCI, or alpha screen) to prevent dissociation of PlGF complexes.
- In some embodiments, PlGF-d is measured at multiple time points in the same subject to determine a change in dissociation rate. For example, PlGF-d may be measured every 2, 4, 6, 8, 12, 18, 24, 36, 48, 96, 120, 144, 168 or more hours.
- In some embodiments, PlGF-f is not measured by preventing dissociation of PlGF complexes but rather by use of an antibody with an alternative epitope. Such an epitope may be an epitope specifically blocked by sFLT (e.g. a mono-mono antibody).
- In some embodiments, PlGF-f is measured by preventing dissociation of other PlGF complexes using addition of a chemical crosslinking agent. Such agents react with labile groups naturally present on protein surfaces (e.g. amino-, sulfhydryl-groups) to covalently stabilize protein-protein interactions. In some embodiments, the crosslinking agent comprises an amine-to-amine crosslinking moiety such as an NHS ester (e.g. DSG, DSS, BS3, TSAT, BS(PEG)5, PS(PEG)9, DSP, DTSSP, DST, BSOCOES, EGS, Sulfo-EGS), an imidoester (e.g. DMA, DMP, DMS, DTBP), or a difluro group (e.g. DFDNB). In some embodiments, the crosslinking agent comprises a sulfhydryl-to-sulfhydryl group crosslinker, such as an NHS-Haloacetyl Crosslinker (e.g. SIA, SBAP, SIAB, Sulfo-SIAB), NHS-maleimide (e.g. AMAS, BMPS, GMBS, sulfo-GMBS, MBS, sulfo-MBS, SMCC, sulfo-SMCC, EMCS, sulfo-EMCS, SMPB, sulfo-SMPB, SMPH, LC-SMCC, sulfo-KMUS), or an NHS-Pyridyldithiol Crosslinker (e.g. SPDP, LC-SPDP, sulfo-LC-SPDP, SMPT, PEG4-SPDP, PEG12-SPDP).
- In some embodiments, the measurement of PlGF-f has a LLOQ of less than or equal to 10 pg/ml, 20 pg/ml, 30 pg/ml, 40 pg/ml, 50 pg/ml, or 100 pg/ml. In some embodiments, the measurement of PlGF-d has a LLOQ of less than or equal to 10 pg/ml, 20 pg/ml, 30 pg/ml, 40 pg/ml, 50 pg/ml, or 100 pg/ml.
- In some aspects, the present disclosure provides for a method for determining a PlGF-f:PlGF-d ratio in a biological sample comprising PlGF, the method comprising: determining an amount of PlGF-f in a first aliquot of the biological sample by: contacting a first aliquot with a capture reagent for a first time at a first temperature, wherein the PIGF-f binds to the capture reagent to form a PIGF-f-capture reagent complex; contacting the PIGF-f-capture reagent complex with a detector reagent for a second time at a second temperature, wherein the PIGF-f-capture reagent complex binds to the second reagent to form a first ternary complex comprising PIGF-f, the capture reagent, and the detector reagent; and detecting the first ternary complex; and determining an amount of PlGF-d in a second aliquot of the biological sample by: contacting a second aliquot with the capture reagent for a third time at a third temperature, wherein the PIGF-d binds to the capture reagent to form a PIGF-d-capture reagent complex; contacting the PIGF-d-capture reagent complex with the detector reagent for a fourth time at a fourth temperature, wherein the PIGF-d-capture reagent complex binds to the second reagent to form a second ternary complex comprising PIGF-d, the capture reagent, and the detector reagent; and detecting the second ternary complex; wherein a PlGF-s-Flt1 complex is stable at the first temperature and the PlGF-s-Flt1 complex dissociates at the second temperature. In some embodiments, the first temperature is less than 30° C., less than 25° C., between 15° C. and 30° C., between 15° C. and 25° C., between 20° C. and 25° C., or about 22° C. In some embodiments, the second temperature is less than 30 C, less than 25 C, between 15 C and 30 C, between 15° C. and 25° C., between 20° C. and 25° C., or about 22° C. In some embodiments, the third temperature is at least 30° C., at least 35° C., between 30° C. and 45° C., about 30° C., about 37° C. or about 45° C. In some embodiments, the fourth temperature is at least 30° C., at least 35° C., between 30° C. and 45° C., about 30° C., about 37° C. or about 45° C. In some embodiments, the first time is less than 1 hour, less than 45 minutes, between 10 minutes and 30 minutes, about 30 minutes, about 20 minutes, or about 15 minutes. In some embodiments, the second time is less than 1 hour, less than 45 minutes, between 10 minutes and 30 minutes, about 30 minutes, about 20 minutes, or about 15 minutes. In some embodiments, the third time is at least 1 hour, between 1 hour and 3 hours, between 90 minutes and 150 minutes, or about 120 minutes. In some embodiments, the fourth time is at least 1 hour, between 1 hour and 3 hours, between 90 minutes and 150 minutes, or about 120 minutes. In some embodiments, the first time is about 30 minutes, the second time is about 30 minutes, the third time is about 2 hours, the fourth time is about 2 hours, the first temperature is about 22° C., the second temperature is about 22° C., the third temperature is about 37° C., and the fourth temperature is about 37° C. In some embodiments, the second step is conducted in the presence of a blocking antibody that prevents s-FLT from rebinding to PlGF.
- Kits
- In some embodiments, the disclosure provides assay kits for analysis of any of the sets of biomarkers included herein for the detection of preeclampsia. In some cases, the assay kits comprise one or more antigen-binding reagents. In some embodiments, the one or more antigen-binding reagents comprise combinations of antigen-binding reagents with specificities for the antigens/biomarkers presented below. In some embodiments, the assay kits comprise one or more antigen-binding reagents in combination with a reagent capable of dissociating a PlGF complex, such as a pH adjustment agent (e.g. a solution of sodium or potassium acetate for acidic adjustment or a solution of bicarbonate or sodium/potassium hydroxide for alkaline adjustment), a surfactant (e.g., an anionic surfactant, a cationic surfactant, a zwitterionic surfactant, a nonionic surfactant, SDS, PFOA, cetrimonium bromide (CTAB), cetylpyridinium chloride (CPC), benzalkonium chloride (BAC), benzethonium chloride (BZT), dimethyldioctadecylammonium chloride, dioctadecyldimethylammonium bromide (DODAB), CHAPS (3[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate), cocamidopropyl hydroxysultaine, Triton-X-100, tween-20, tween-40- tween-60, or tween-80, or any combination thereof), a chaotropic agent (e.g., urea or guanidinium hydrochloride, or a combination thereof), an ionic strength adjustment agent (e.g. a concentrated solution of NaCl, KCl, or ammonium chloride, or a combination thereof) or a steric agent (e.g an antibody, antibody fragment or derivative, aptamer, peptide, protein, or other agent that binds an epitope of free PlGF or free sFLT, PlGF, or sFLT1 and prevent reassembly of a PlGF/sFLT1 complex). In some embodiments, the assay kits comprise one or more antigen-binding reagents described herein in combination with: (a) an antibody, antibody fragment or derivative, aptamer, peptide, protein, or other agent that binds an epitope that reports total (e.g. free plus dissociated) PlGF; and/or (b) an antibody, antibody fragment or derivative, aptamer, peptide, protein, or other agent that binds an epitope that reports free (e.g. without binding to dissociated) PlGF. In some embodiments the reagent capable of dissociating a PlGF complex is provided in the form of a concentrated solution. In some embodiments the concentrated solution consists essentially of the reagent. In some embodiments, the concentrated solution has a concentration of the reagent of at least 50 mM, at least 100 mM, at least 150 mM, at least 200 mM, at least 250 mM, at least 300 mM, at least 350 mM, at least 400 mM, at least 450 mM, at least 500 mM, at least 550 mM, at least 600 mM, at least 650 mM, at least 700 mM, at least 750 mM, at least 800 mM, at least 850 mM, at least 900 mM, at least 950 mM, at least 1 M, at least 2 M, at least 4M, or at least 5 M.
- In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for one or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1 or Endoglin; and (b) PlGF (free, dissociated, or % free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1 or Endoglin; (b) PlGF (free, dissociated, or % free); and (c) KIM1. In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1 or Endoglin; (b) PlGF (free, dissociated, or % free); (c) KIM1; and (d) FGF21. In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1 or Endoglin; (b) PlGF (free, dissociated, or % free); (c) KIM1; and (d) Decorin. In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1 or Endoglin; (b) PlGF (free, dissociated, or % free); (c) KIM1; (d) FGF21; and (e) Decorin.
- In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1; (b) Endoglin; and (c) PlGF (free, dissociated, or % free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1; (b) Endoglin; (c) PlGF (free, dissociated, or % free); and (d) KIM1. In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1; (b) Endoglin; (c) PlGF (free, dissociated, or % free); (d) KIM1; and (e) FGF21. In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1; (b) Endoglin; (c) PlGF (free, dissociated, or % free); (d) KIM1; and (e) Decorin. In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for: (a) sFLT1; (b) Endoglin; (c) PlGF (free, dissociated, or % free); (d) KIM1; (e) FGF21; and (f) Decorin.
- In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for two or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for three or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for four or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for five or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for six or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for seven or more biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for all of sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for two biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for three biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for four biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for five biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for six biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for seven biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for no more than two biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for no more than three biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for no more than four biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for no more than five biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for no more than six biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free). In some embodiments, the one or more antigen-binding reagents comprise antigen-binding reagents with specificities for no more than seven biomarkers selected from sFLT, free sFLT, Endoglin, KIM-1, FGF21, Decorin, Clec4A and CD274 in combination with PlGF(f), PlGF(d), or PlGF (% free).
- In some embodiments, the assay kit provided is suitable for a multiplex homogenous biomarker assay, suitable for detection of all the analytes in a single reaction (e.g. in the same solution compartment). In such assays, multiple antibodies or antigen detection reagents that bind to separate epitopes are provided against the same analyte/biomarker, and detection of coincident binding/interaction of both antibodies to the same molecule of analyte/biomarker serves to detect the analyte/biomarker in the sample. Thus, such kits provide two antibodies or antigen-binding reagents against each analyte.
- In the case of a multiplex homogenous biomarker assay detectable by TR-FRET, such kits provide a pair of antibodies or antigen-binding reagents for each analyte conjugated to a complementary pair of FRET dyes, wherein one antibody or antigen-binding reagent of the pair is conjugated to a FRET donor and the other is conjugated to a FRET acceptor. In the case of a multiplex homogenous biomarker assay detectable by LOCI, such kits provide a pair of antibodies or antigen-binding reagents wherein one antibody or antigen-binding reagent of the pair is conjugated to a photosensitizer and the other antibody or antigen-binding reagent of the pair is conjugated to an oxygen sensitive dye.
- In some embodiments, the assay kit provided is suitable for a multiplex non-homogenous biomarker assay suitable for detection of all the analytes in separate reactions (e.g. in separate solution compartments). In some embodiments of such assays (e.g. sandwich ELISA), antibodies or antigen binding reagents against the relevant set of biomarkers are provided attached to a substrate (e.g. in a well of a multiwell plate, or in a lateral flow assay lane). A second free antibody against each of the biomarkers provided attached to the substrate is also provided; this antibody can be labeled (e.g. with a fluorescent dye, with a chemiluminescent enzyme, or a luminescent enzyme) or unlabeled. In the case where an unlabeled antibody is provided, a secondary labeled (e.g. with a fluorescent dye, with a chemiluminescent enzyme, or a luminescent enzyme) antibody or antigen-binding reagent is provided which has binding specificity against the second free antibody.
- In some embodiments, the kit is for use as an in vitro diagnostic kit that includes one or more cartridges with reagents for testing on third-party platforms. Data collected from third-parties could be uploaded to a server (the cloud), put through a model/algorithm, and results can be shared with a doctor or other medical practitioner. Kits may also include instructions for use (e.g., according to any of the methods described herein).
- In some embodiments, the assay kits comprise apparatus for collecting a biological sample from a pregnant female subject. Such apparatus can include, but is not limited to, an ophthalmic sponge or collection tube, with or without any reagents or preservatives necessary for sample preparation or stable storage (e.g. any of anti-microbials such as sodium azide and sodium fluoride, anti-coagulants such as EDTA, sodium citrate, potassium oxalate, heparin, and acid citrate dextrose, or fixatives such as methanol, ethanol, and formaldehyde). Collection tubes may be configured for or suitable for collection of any of the samples described herein, such as blood, peripheral blood, serum, plasma, urine, cervicovaginal mucus, or saliva. Collection tubes include e.g., red-top tubes; mottled red/gray-top, gold-top, or cherry red-top (gel-barrier) tubes; lavender-top tubes, and gray-top tubes.
- In some embodiments, the assay kits comprise apparatus facilitating any of the assays described herein. Such apparatus can include, but is not limited to, lateral flow strips or multiwell plates containing any of the antibodies or antigen-binding fragments thereof described herein conjugated or adsorbed to a surface thereof.
- In some embodiments, the assay kits comprise instructions for carrying out any of the assays described herein. Such assays include immunoassays, ELISA assays, sandwich ELISA assays, TR-FRET assays, proximity extension assays, amplified luminescent proximity (LOCI) assays, or luminescent oxygen channeling immunoassays.
- In some cases, the assay kits comprise no more than 20, no more than 15, no more than 10, no more than 9, no more than 8, no more than 7, no more than 6, nor more than 5, no more than 4, no more than 3, no more than 2, or no more than 1 probes, antibodies, or antigen binding fragments or derivatives of antibodies specific for any of the biomarkers described herein.
- During the development of an ELISA capture assay for PlGF from patient sera, it was discovered that there was a divergence in values measured from samples using different assay protocols. This divergence was only in samples with a high sFLT concentration. Further testing identified the assay temperature as a putative source of the divergence: room temperature and 30° C. incubations yielded divergent results.
- The dependence of the phenomenon on temperature and sFLT concentration indicated the phenomenon might be the result of dissociation of PlGF complexes at higher assay temperatures. Specifically, it was hypothesized that variable dissociation of PlGF:sFLT 1:1 and PlGF:sFLT 2:1 complexes during the ELISA capture assay at high temperature might lead to artificial increases in the measured “free PlGF” during the assay protocol (see
FIG. 1A andFIG. 1B ). - Accordingly, experiments were performed to directly determine the relationship between temperature, time, sFLT levels, and measured PlGF levels. In a first experiment, serum samples from pregnant women identified as preeclamptic (PE) or non-preeclamptic (non-PE) by later clinical outcome were exposed measured by ELISA at various temperatures between 15° C. and 37° C. during incubation with PlGF capture antibodies, as well as after washing and during incubation with PlGF labeled detection antibody (see
FIG. 2A ). ELISAs performed at 37° C. showed higher detected PlGF concentrations than those performed at 15° C., and this effect was stronger in PE samples (which tend toward higher sFLT concentrations). - These data indicated that % free PlGF values (measured as a ratio of “naturally” free PlGF versus in-vitro dissociated PlGF) could be measured using this dissociation phenomenon and have value in generating more accurate PlGF-based prediction of preeclamptic patients.
- Accordingly, experiments were first performed on third-trimester pregnant patient serum samples naturally containing various quantities of sFLT (e.g. 1-64 ng/ml sFLT and applying various incubation conditions (2 hours capture at 37° C. or 45° C., while keeping incubation with detection antibody after wash at 22° C.) to the serum samples to optimize capture incubation time (see
FIG. 3A ). Samples with “high” sFLT (>10 ng/ml, which would favor formation of the 1:1 and 2:1 PlGF:sFLT complex) showed larger % changes in detected PlGF concentration than samples with “low” sFLT (<10 ng/ml, which would favor “free” PlGF) sFLT when incubated at 37° C., and incubation at a higher temperature such as 45° C. showed minimal additional benefit (seeFIG. 3B ). This indicated that 37° C. was adequate to dissociate PlGF heteromultimers in solution. - Next, incubation time for capture was optimized. First, a separate set of third-trimester serum samples also containing variable sFLT concentrations as above (e.g. 1-64 ng/ml sFLT) to capture incubations at 37° C. for various different incubation times, varying capture and post-wash detection incubation times simultaneously (see
FIG. 4A , where the x-axis shows the incubation time used for both the PlGF capture and detection incubation). While samples with sFLT<4 ng/ml and >25 ng/ml diverged in measured PlGF concentrations at short incubation times, measured PlGF concentrations in all samples converged when the incubation was extended to 120 minutes, indicating that a capture time of 120 minutes was adequate to dissociate and capture PlGF from PlGF:sFLT complexes. Second, because of the promising results at 120 minutes, the mixtures of PlGF and sFLT were subjected to additional capture time/detection time combinations including an overnight capture incubation (seeFIG. 4B , where the times are listed in order of capture time/detection incubation time; andFIG. 4C ); this demonstrated that overnight capture may have some advantages over 120 min but that the differences are minimal. Third, capture incubation times for solutions containing only recombinant PlGF of different concentrations was measured, to verify that the capture times determined for the PlGF:sFLT mixtures was also applicable to measure PlGF alone with maximal sensitivity (seeFIG. 4D ) When capture incubation times were tested for various PlGF concentrations, it was also found that a 2 hr incubation maximized sensitivity for measuring PlGF. - Standard protocols for PlGF-f and PlGF-d quantification were established based on the results of temperature and time optimization. PlGF-f is measured at less than 22° C. with a 30-minute capture incubation a 30-minute detector incubation; and PlGF-d is measured at 37° C. with a 2-hour capture incubation and a 2-hour detector incubation. For comparison, the legacy PlGF ELISA protocol (PlGF(II)) had intermediate conditions with a 1-hour capture incubation and a 1-hour detector incubation is measured at 30° C.
- Finally, using these optimized conditions, PlGF (free) and PlGF (dissociated) conditions were measured from patient serum samples (see
FIG. 5A , where the samples are segregated into high and low sFLT concentrations in the left and right panels). As, expected, more PlGF was detected in the PlGF(d) conditions, especially at higher sFLT concentrations. Accordingly, this protocol was applied to measure PlGF-f:PlGF-d ratios in a set of pregnant patient serum samples containing (n=10) preeclamptic samples and (n=15) non-preeclamptic samples (seeFIG. 5B , where PlGF ratio is plotted versus sFLT concentration). Measurement of the PlGF f:d ratio in these samples revealed a population of low-sFLT, non-PE samples (see leftmost red dots inFIG. 5B ), and also provided separation between PE samples and higher sFLT non-PE samples, indicating that the f:d ratio may improve of the ability to discriminate between PE and non-PE populations in a screening test compared to the PlGF/sFLT ratio used in a commercially available screening test. - Based on the results in a limited set of pregnant patient PE/non-PE samples, the PlGF f:d ratio was determined for a larger set of PE-nonPE pregnant patient samples from two recent studies (PE-104 and PE-105, described herein, see Example 3).
FIG. 6 shows plots of PE and non-PE patients from the studies segregated using PlGF(f) conc or % free PlGF ((f)/(d)×100) demonstrating that both show improved separation of PE samples from non-PE samples.FIG. 7A shows the −f and −d levels measured for preeclamptic and non-preeclamptic patients alongside results from the legacy PlGF assay (“PlGF(II)”, which was determined above to measure intermediate concentrations of dissociated PlGF). PlGF(f) provided better discrimination between PE and non-PE samples than PlGF(II) or PlGF(d) individually. Graphing the same data as free PlGF versus sFLT (FIG. 7B ) and % free PlGF vs sFLT (FIG. 7C ) demonstrated that use of a PlGF f:d ratio (such as % free PlGF) increased the dynamic range of PlGF measurement between non-PE and PE samples and improved their separation (see blue vs orange dots inFIGS. 7B and 7C ). - Accordingly, formal performance statistics including were calculated for PlGF(f), PlGF(d), PlGF (% f), and PlGF(II) (the old PlGF measurement protocol) to discriminate PE from non-PE pregnant patient serum samples using this data set (see
FIG. 8 ). Use of either the PlGF(f) or PlGF (% f) showed improvements of both AUC and AUP versus the previous assay conditions that measured an indeterminate amount of PlGF(f) and PlGF(d), indicating that the free and percent free PlGF metrics may add additional discrimination power to biomarker assays using PlGF to detect preeclampsia. - Lastly, an analysis of PlGF(f), PlGF(d), and PlGF (% free) was performed versus gestational age using pregnant patient serum samples from the PE-104 and PE-105 studies (see
FIG. 10 ). The graphs demonstrate that free PlGF improves segregation of non-PE/PE samples into earlier gestational age (<28 weeks), indicating that the marker could further enable stratification of the disease cohort at earlier gestational ages. Further, the graphs indicate that the amount of dissociated PlGF is not a function of the gestational age, and that onset of the disease may be triggered by dissociation/cleavage of FLT from the cell surface into the free form (sFlt) which subsequently binds to PlGF and results in reduction of free PlGF. Finally, the graphs indicate that as the free PLGF naturally decreases from 30 weeks gestational age onward, such information can further be used to control the false positive rate when interrogating the serum for free PlGF. - Blood samples are collected from pregnant patients using red top blood collection tubes; the tubes are allowed to clot at room temperature for 30-60 minutes, are centrifuged 20 minutes at 1300 g to remove the clot and are aliquoted for long term storage at below −80° C.
- Measurement of PlGF (Free)
- Blood samples are collected from pregnant patients using red top blood collection tubes; the tubes are allowed to clot at room temperature for 30-60 minutes, are centrifuged 20 minutes at 1300 g to remove the clot and are aliquoted for long term storage below −80° C.
- Measurement of PlGF (Free)
- R&D Systems Quantikine kit (Catalog number DPG00) is used to measure the free form of PlGF [PlGF(f)]. In brief, 100 ul of assay diluent RD1-22 (part #895490) is added to each well of the PlGF microplate coated with the monoclonal anti-PlGF antibody (part #890509). Each sample is diluted 1:2 in calibrator diluent RD6-11 (part #895489) and 100 ul of a diluted serum sample is aliquoted into each well of the 96-well plate. The serum samples are incubated in the plate with low shaking for 30 minutes at ambient temperature. Following incubation, the sample from each well of the plate was aspirated and the well was not washed. During a second incubation addition of 200 ul of PlGF conjugate (part #890510) per well is incubated with low shaking for 30 minutes at ambient temperature, followed by washing 4 times with 400 ul of wash solution. This is followed by adding 200 ul TMB substrate solution (Enhanced K-Blue TMB from Neogen, part #308177) in each well and left to incubate for 30 minutes at ambient temperature with low shaking. Finally, the reaction is stopped with the addition of 50 ul of stop solution (2N sulfuric acid from Honeywell part #35276-1L) and the optical density of each well is measured using a microplate reader set at 450 nm.
- Measurement of PlGF (Dissociated)
- R&D Systems Quantikine kit (Catalog number DPG00) is used to measure the dissociated form of PlGF (PlGF(d)]. In brief, 100 ul of assay diluent RD1-22 (part #895490) is added to each well of the PlGF microplate coated with the monoclonal anti-PlGF antibody (part #890509). Each sample is diluted 1:2 in calibrator diluent RD6-11 (part #895489) and 100 ul of a diluted serum sample is aliquoted into each well of the 96-well plate. The serum samples are incubated in the plate with low shaking for 120 minutes at 37° C. Following incubation, the sample from each well of the plate was aspirated and the well was not washed. During a second incubation addition of 200 ul of PlGF conjugate (part #890510) per well is incubated with low shaking for 120 minutes at 37° C., followed by washing 4 times with 400 ul of wash solution. This if followed by adding 200 ul TMB substrate solution (Enhanced K-Blue TMB from Neogen, part #308177) in each well and left to incubate for 20 minutes at 37°C with low shaking. Finally, the reaction is stopped with the addition of 50 ul of stop solution (2N sulfuric acid from Honeywell part #35276-1L) and the optical density of each well is measured using a microplate reader set at 450 nm.
- Samples from two studies were used to develop embodiments of new, improved PlGF assays described herein: PRO-105 and PRO-104.
- PRO-105: Pregnant women who were 18 years or older (20 weeks to 39 weeks of gestation at first visit) with suspected preeclampsia (based on new onset symptoms, elevated blood pressure, proteinuria, edema or others) were selected for participation in the PRO-105 study. Baseline procedures were performed, including collection of demographic, medical and obstetric histories, list of concomitant medications, weight, height, blood pressure, and other clinical information, as well as obtaining blood and urine samples for use in biomarker assays. After discharge, all patients in the study were followed by interim research visits every 14 days (+/−3 days). For patients who developed PreE, the time (in days) from baseline sampling, the gestational age at diagnosis, and the severity of the disease was recorded. Patients who did not develop preeclampsia before or at delivery were included in the NEGATIVE-PRE-E CONTROL (NonPreE) group. For these NEGATIVE PRE-E CONTROLS, the time from baseline sampling (in days) to either delivery or loss to follow-up was recorded.
- The interim study visits occurred every 14 days [+/−3 days] and continued until the subject either: 1) reached 37 weeks' gestation, 2) developed PreE, 3) delivered, or 4) was lost to follow-up.
- Delivery outcomes (maternal and neonatal clinical information) were collected on all subjects enrolled in the study. Additionally, if possible, during admission for delivery, blood and urine samples were collected for analysis at delivery.
- The study demographics are presented below in Table 1A:
-
TABLE 1A Demographic and Clinical Variables for PRO-105 Study Clinical Study Demographic and Clinical Variables PRO-105 (n = 1277) Missing 18 2/7 to 27 6/7 28 0/7 to 34 6/7 35 0/7 to 36 6/7 Demographic (n = 12) (n = 337) (n = 550) (n = 378) Maternal Age (median, IQR) NA 30 (8) 29 (8) 30 (9) under 25 0 (0.00%) 67 (19.88%) 124 (22.55%) 73 (19.31%) 25 to 29 0 (0.00%) 88 (26.11%) 172 (31.27%) 108 (28.57%) 30 to 34 0 (0.00%) 101 (29.97%) 148 (26.91%) 100 (26.46%) 35 to 39 0 (0.00%) 64 (18.99%) 88 (16.00%) 75 (19.84%) 40 to 45 0 (0.00%) 17 (5.04%) 18 (3.27%) 22 (5.82%) Missing 12 (100.00%) 0 (0.00%) 0 (0.00%) 0 (0.00%) BMI (median, IQR) NA 36.21 (12.63) 35.88 (11.28) 37.44 (10.93) Underweight (<18.5) 0 (0.00%) 0 (0.00%) 0 (0.00%) 0 (0.00%) Normal (18.5 through 24 BMI) 0 (0.00%) 25 (7.42%) 29 (5.27%) 20 (5.29%) Overweight (25 through 29 BMI) 0 (0.00%) 59 (17.51%) 88 (16.00%) 58 (15.34%) Obese - class 1 (30 through 34 BMI) 0 (0.00%) 71 (21.07%) 132 (24.00%) 69 (18.25%) Obese - class 2 (35 through 39 BMI) 0 (0.00%) 59 (17.51%) 129 (23.45%) 98 (25.93%) Obese - class 3 (40 or greater BMI) 0 (0.00%) 123 (36.50%) 170 (30.91%) 132 (34.92%) Missing 12 (100.00%) 0 (0.00%) 2 (0.36%) 1 (0.26%) Race American Indian or Alaskan Native 0 (0.00%) 1 (0.30%) 1 (0.18%) 0 (0.00%) Asian 0 (0.00%) 3 (0.89%) 7 (1.27%) 7 (1.85%) Black or African American 0 (0.00%) 114 (33.83%) 194 (35.27%) 143 (37.83%) Native Hawaiian or Other Pacific Islander 0 (0.00%) 1 (0.30%) 0 (0.00%) 1 (0.26%) White 0 (0.00%) 204 (60.53%) 335 (60.91%) 221 (58.47%) Two or more races 0 (0.00%) 0 (0.00%) 0 (0.00%) 0 (0.00%) Unknown 0 (0.00%) 13 (3.86%) 12 (2.18%) 6 (1.59%) Refused 0 (0.00%) 1 (0.30%) 1 (0.18%) 0 (0.00%) Ethnicity Hispanic or Latino 0 (0.00%) 10 (2.97%) 16 (2.91%) 13 (3.44%) Not Hispanic or Latino 0 (0.00%) 214 (63.50%) 361 (65.64%) 273 (72.22%) Unknown/Not Provided 12 (100.00%) 113 (33.53%) 173 (31.45%) 92 (24.34%) Pregnancy History Gestational Age (GA) at Enrollment (median, IQR) NA 23.71 (4.29) 32 (3.29) 36.71 (1.85) <28 0/7 0 (0.00%) 337 (100.00%) 0 (0.00%) 0 (0.00%) 28 0/7 to 28 6/7 0 (0.00%) 0 (0.00%) 72 (13.09%) 0 (0.00%) 29 0/7 to 29 6/7 0 (0.00%) 0 (0.00%) 55 (10.00%) 0 (0.00%) 30 0/7 to 30 6/7 0 (0.00%) 0 (0.00%) 65 (11.82%) 0 (0.00%) 31 0/7 to 31 6/7 0 (0.00%) 0 (0.00%) 75 (13.64%) 0 (0.00%) 32 0/7 to 32 6/7 0 (0.00%) 0 (0.00%) 95 (17.27%) 0 (0.00%) 33 0/7 to 33 6/7 0 (0.00%) 0 (0.00%) 87 (15.82%) 0 (0.00%) 34 0/7 to 34 6/7 0 (0.00%) 0 (0.00%) 101 (18.36%) 0 (0.00%) 35 0/7 to 35 6/7 0 (0.00%) 0 (0.00%) 0 (0.00%) 96 (25.40%) 36 0/7 to 36 6/7 0 (0.00%) 0 (0.00%) 0 (0.00%) 112 (29.63%) >=37 0/7 0 (0.00%) 0 (0.00%) 0 (0.00%) 170 (44.97%) Missing 12 (100.00%) 0 (0.00%) 0 (0.00%) 0 (0.00%) Gravidity (median, IQR) NA 3 (3) 2 (2) 2 (3) 1 0 (0.00%) 39 (11.57%) 113 (20.55%) 96 (25.40%) >1 0 (0.00%) 238 (70.62%) 350 (63.64%) 256 (67.72%) Missing 12 (100.00%) 60 (17.80%) 87 (15.82%) 26 (6.88%) # of births (median, IQR) NA 1 (2) 1 (2) 1 (2) Nulliparous (0) 0 (0.00%) 59 (17.51%) 138 (25.09%) 123 (32.54%) >0 0 (0.00%) 144 (42.73%) 217 (39.45%) 138 (36.51%) Missing 12 (100.00%) 134 (39.76%) 195 (35.45%) 117 (30.95%) History of Previous Preeclampsia NA 77 (22.85%) 95 (17.27%) 49 (12.96%) Paternity Match Yes NA NA NA NA No NA NA NA NA Unknown NA NA NA NA N/A NA NA NA NA Type of Pregnancy Spontaneous NA NA NA NA Assisted Reproductive Technology NA NA NA NA Fetal Reduction NA NA NA NA Vanishing Twin Syndrome NA NA NA NA Medical History and Ongoing Conditions Chronic Hypertension (CHTN) NA 147 (43.62%) 151 (27.45%) 118 (31.22%) Gestational Hypertension (GHTN) NA NA NA NA Diabetes Type I NA 11 (3.27%) 20 (3.64%) 8 (2.12%) Type II NA 41 (12.17%) 35 (6.36%) 22 (5.82%) Gestational Diabetes Type A1 NA NA NA NA Type A2 NA NA NA NA Smoker Current NA NA NA NA Past NA NA NA NA Autoimmune Disease NA 12 (3.56%) 26 (4.73%) 12 (3.17%) Nephropathy/Kidney Disease NA 4 (1.19%) 6 (1.09%) 1 (0.26%) Antiphospholipid Syndrome NA 0 (0.00%) 2 (0.36%) 1 (0.26%) Cervical Dysplasia NA NA NA NA Endometriosis NA NA NA NA Labor and Delivery Type of Labor No labor NA NA NA NA Spontaneous NA NA NA NA Augmented NA NA NA NA Induced 3 (25.00%) 105 (31.16%) 194 (35.27%) 175 (46.42%) Mode of Delivery Cesarean 7 (58.33%) 165 (48.96%) 223 (40.55%) 171 (45.24%) Vaginal 3 (25.00%) 134 (39.77%) 256 (46.54%) 179 (47.48%) NA 2 (16.67%) 38 (11.28%) 71 (12.91%) 28 (7.41%) Birth Weight in Grams (median, IQR) NA NA NA NA Size for Gestational Age Small for GA (SGA) NA NA NA NA Appropriate for GA (AGA) NA NA NA NA Large for GA (LGA) NA NA NA NA GA at Delivery (median, IQR) 36.86 (1.71) 38.29 (2.28) 38.14 (2.28) 38.71 (1.72) Preterm (<37 weeks' GA) 5 (41.67%) 86 (25.52%) 134 (24.36%) 23 (6.08%) Full-term (≥37 weeks' GA) 5 (41.67%) 238 (70.62%) 399 (72.54%) 348 (92.06%) Missing 2 (16.67%) 13 (3.86%) 17 (3.10%) 7 (1.85%) APGAR (median, IQR) 1 min NA NA NA NA 5 min NA NA NA NA Intrauterine Fetal Demise (IUFD) 0 (0.00%) 4 (1.19%) 3 (0.55%) 0 (0.00%) - PRO-104: Select samples from PRO-104 were used as positive controls to further develop the assay. Pregnant women who were 18 to 45 years of age with 32
weeks 0 days to 36 weeks and 6 days weeks of gestation age who presented at clinical sites with signs and symptoms of preeclampsia (based on new onset or worsening of symptoms including elevated blood pressure, proteinuria, edema amongst others) were eligible for participation in the PRO-104 study. Baseline procedures were performed, including collection of demographic, medical and obstetric histories, list of concomitant medications, weight, height, blood pressure, and other clinical information, as well as obtaining blood and urine samples for use in biomarker assays. Study subjects were followed by several study visits until the delivery visit. - Study subjects who were diagnosed with PreE, within the first 24 hours of enrollment were included in the POSITIVE CONTROL cohort.
- Subjects who did not develop preeclampsia before or at delivery or subjects who were diagnosed with PreE after 24 h of enrollment were included in the NEGATIVE-PRE-E CONTROL (NonPreE) cohort. For all subjects, the time from baseline sampling (in days) to either delivery or loss to follow-up was recorded.
- Delivery outcomes (maternal and neonatal clinical information) were collected on all subjects enrolled in the study. Additionally, if possible, during admission for delivery, blood and urine samples were collected for analysis at delivery.
- PlGF-f, PlGF-d, sFlt are measured in serum samples from pregnant women. VEGF is additionally measured in the serum from pregnant women with high PIGF-d, and/or high sFlt. VEGF above 10 pgs/ml or 50 pgs/ml or 100 pgs/ml is indicative of a reduced risk of preeclampsia.
- Threshold analysis was performed to identify a threshold for a univariate model utilizing % PlGFfree (PlGFfree/PlGFdissociated) as a predictor of preeclampsia or absence thereof with a single cutoff using the PlGFfree/PlGFdissociated protocols described in Example 2 (in this model, % PlGFfree levels higher than the threshold indicate preeclampsia). An AUC curve analysis of % PlGFfree in the PRO-105 study populations from Example 3 were performed (see
FIG. 12 ). 100 repeats of 10-fold cross-validation on the data from the study population was used to identify a threshold that achieved 90% sensitivity on the study population; the threshold was set at the 25th percentile of the 100 repeats of 10-fold cross validation. The 25th percentile threshold (Q25 Threshold) was determined to be ˜0.046; this threshold alongside other threshold distribution data is summarized below and inFIG. 13 : -
Median MAD IQR Q25 Mean SD CV Threshold Threshold Threshold Threshold Specificity Specificity Specificity 0.0475049 0.0022809 0.0030798 0.045961 0.7027771 0.0058415 0.0083121 - With this model locked at the defined Q25 threshold, the performance of the model was tested in a cross-sectional analysis on the PRO-105 study population, segregating the samples from the study into either “positive Pre-E controls” or “non-cases” as illustrated in
FIG. 15A . - The performance parameters for this univariate model-based analysis applying the Q25 threshold for a cross-sectional analysis are displayed in Table 1A below:
-
N TP TN FP FN Sensitivity Specificity Prevalence PPV NPV 458 86 238 124 10 89.6 65.8 10% 22.5 98.3 [81.9-94.2] [60.7-70.4] [17.4-28.6] [95.8-99.3] - Surprisingly, this model utilizing both PlGF-d and PlGF-f exhibited high performance in terms of NPV.
- Next, with this model locked at the defined Q25 threshold, the performance of the model was tested in a forward-looking analysis in a new study cohort recruited and designated PRO-129. The target population for the study was pregnant women 18 to 45 years of age and 28 to 36 6/7 weeks gestational age who presented with signs and symptoms of PE, including one or more of: (i) new onset increased blood pressure in an otherwise normotensive patient; (ii) worsening hypertension in a patient with pre-existing hypertension; (iii) new onset proteinuria or worsening of pre-existing proteinuria; (iv) any other clinical finding typically associated with suspicion of preeclampsia and requiring workup to rule-out preeclampsia. The study demographics are presented below in Table B.
-
TABLE B Demographic and Clinical Variables for PRO-129 Study Gestation at Enrollment (weeks, days) Demographic and Clinical Variables <35 0/7 35 0/7 to 36 6/7 (n = 269) (n = 135) Demographic n (%) Maternal Age (median, IQR) 30 (8.00) 30 (10.00) under 25 50 (18.59%) 27 (20.00%) 25 to 29 78 (29.00%) 37 (27.41%) 30 to 34 80 (29.74%) 36 (26.67%) 35 to 39 47 (17.47%) 25 (18.52%) 40 to 45 14 (5.20%) 10 (7.41%) BMI (mean, SD) 35.16 (10.90) 34.75 (9.32) Underweight (<18.5) 0 (0.00%) 0 (0.00%) Normal (18.5 through 24 BMI) 11 (4.09%) 7 (5.19%) Overweight (25 through 29 BMI) 51 (18.96%) 27 (20.00%) Obese - class 1 (30 through 34 BMI) 70 (26.02%) 36 (26.67%) Obese - class 2 (35 through 39 BMI) 59 (21.93%) 32 (23.70%) Obese - class 3 (40 or greater BMI) 77 (28.62%) 33 (24.44%) Missing 1 (0.37%) 0 (0.00%) Race American Indian or Alaskan Native 2 (0.74%) 1 (0.74%) Asian 12 (4.46%) 3 (2.22%) Black or African American 42 (15.61%) 18 (13.33%) Native Hawaiian or Other Pacific Islander 0 (0.00%) 0 (0.00%) White 182 (67.66%) 100 (74.07%) Two or more races 6 (2.23%) 0 (0.00%) Unknown 22 (8.18%) 13 (9.63%) Refused 3 (1.12%) 0 (0.00%) Ethnicity Hispanic or Latino 55 (20.45%) 35 (25.93%) Not Hispanic or Latino 200 (74.35%) 94 (69.63%) Unknown/Not Provided 14 (5.20%) 6 (4.44%) Pregnancy History Gestational Age (GA) 32.14 (3.28) 36 (1.00) at Enrollment (median, IQR) 28 0/7 to 28 6/7 32 (11.90%) 0 (0.00%) 29 0/7 to 29 6/7 18 (6.69%) 0 (0.00%) 30 0/7 to 30 6/7 31 (11.52%) 0 (0.00%) 31 0/7 to 31 6/7 43 (15.99%) 0 (0.00%) 32 0/7 to 32 6/7 45 (16.73%) 0 (0.00%) 33 0/7 to 33 6/7 46 (17.10%) 0 (0.00%) 34 0/7 to 34 6/7 54 (20.07%) 0 (0.00%) 35 0/7 to 35 6/7 0 (0.00%) 59 (43.70%) 36 0/7 to 36 6/7 0 (0.00%) 76 (56.30%) Gravidity (median, IQR) 2 (3) 2 (2) 1 71 (26.39%) 39 (28.89%) >1 198 (73.61%) 96 (71.11%) # of births (median, IQR) 1 (2) 1 (1) Nulliparous (0) 93 (34.57%) 60 (44.44%) >0 176 (65.43%) 75 (55.56%) History of Previous Preeclampsia 47 (17.47%) 22 (16.30%) Chronic Hypertension (CHTN) 53 (19.70%) 20 (14.81%) Gestational Hypertension (GHTN) 98 (36.43%) 52 (38.52%) Diabetes Type I 3 (1.12%) 1 (0.74%) Type II 18 (6.69%) 10 (7.41%) Gestational Diabetes Type A1 19 (7.06%) 6 (4.44%) Type A2 23 (8.55%) 16 (11.85%) Smoker Current 12 (4.46%) 3 (2.22%) Past 39 (14.50%) 15 (11.11%) Nephropathy/Kidney Disease 4 (1.49%) 1 (0.74%) Antiphospholipid Syndrome 0 (0.00%) 0 (0.00%) Cancer Lumpectomy 1 (0.37%) 0 (0.00%) Melanoma 0 (0.00%) 1 (0.74%) Thyroid Cancer 2 (0.074%) 0 (0.00%) Cervical Dysplasia 3 (1.12%) 1 (0.74%) Endometriosis 5 (1.86%) 0 (0.00%) Focal Segmental Glomerulosclerosis 1 (0.37%) 0 (0.00%) Labor and Delivery Type of Labor No labor 94 (34.94%) 28 (20.74%) Spontaneous 64 (23.79%) 27 (20.00%) Augmented 5 (1.86%) 12 (8.89%) Induced 106 (39.41%) 68 (50.37%) Mode of Delivery Cesarean 143 (53.16%) 53 (39.26%) Vaginal 126 (46.84%) 82 (60.74%) Birth Weight in Grams (median, IQR) 3130 (862) 3138.5 (730) Size for Gestational Age Small for GA (SGA) Appropriate for GA (AGA) Large for GA (LGA) GA at Delivery (median, IQR) 37.71 (2.57) 37.86 (2.00) Preterm (<37 weeks' GA) 78 (29.00%) 24 (17.78%) Full-term (≥37 weeks' GA) 191 (71.00%) 111 (82.22%) APGAR (median, IQR) 1 min 8 (1) 8 (1) 5 min 9 (0) 9 (0) Intrauterine Fetal Demise (IUFD) 0 (0.00%) 0 (0.00%) - As part of this forward-looking rule out analysis, the samples from the PRO-129 study were re-segregated into either “cases” or “non-cases” as shown in
FIG. 15B . As part of this scheme, the clinical status of a subject is considered “case” if the preeclampsia diagnosis happens within the rule-out window and subject delivers before 37 0/7 weeks of gestation, and the clinical status of a subject is considered as ‘non-case’ if no diagnosis of Preeclampsia happens during the pregnancy or diagnosis of preeclampsia happens outside of the rule-out window or the diagnosis of preeclampsia happens within the rule-out-window but the subject delivers after 36 6/7 weeks of gestation - As part of this forward-looking rule out analysis, the same % PLGFfree-based univariate model above with the Q25 threshold locked was used to rule-out preeclampsia when the % PLGFfree value was below the threshold. The performance parameters for this univariate model-based analysis applying a “rule out” window of 14 days based on the Q25 threshold are described in Table 1B below:
-
TABLE 1B Performance Of % PLGFfree Model For Ruling Out Preeclampsia Over A Window Of 14 Days N TP TN FP FN Sensitivity Specificity Prevalence PPV NPV 350 44 179 121 6 88.0 59.7 10% 19.5 97.8 [76.2-94.4] [54.0-65.1] [14.2-26.2] [94.5-99.1] - Surprisingly, this model utilizing both PlGF-d and PlGF-f exhibited high performance even at a relatively stringent task—ruling out future preeclampsia in patients over a window of 14 days.
- While preferred embodiments of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in practicing the invention. It is intended that the following claims define the scope of the invention and that methods and structures within the scope of these claims and their equivalents be covered thereby.
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20080206889A1 (en) * | 2007-02-26 | 2008-08-28 | Response Biomedical Corporation | Comparative multiple analyte assay |
WO2009155324A2 (en) * | 2008-06-18 | 2009-12-23 | Abbott Laboratories | Pigf-1 assay and kits and components thereof |
Family Cites Families (200)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4777964A (en) | 1986-01-02 | 1988-10-18 | David Briggs | System for obtaining blood samples and submitting for testing of aids |
US5223440A (en) | 1987-11-17 | 1993-06-29 | Adeza Biomedical Corporation | Ex vivo product of conception test to determine abortion |
US5096830A (en) | 1987-11-17 | 1992-03-17 | Adeza Biomedical Corporation | Preterm labor and membrane rupture test |
US5281522A (en) | 1988-09-15 | 1994-01-25 | Adeza Biomedical Corporation | Reagents and kits for determination of fetal fibronectin in a vaginal sample |
US4919889A (en) | 1988-09-15 | 1990-04-24 | Aspen Diagnostics Corporation | Sample collection and transport fluid composition |
JP3222135B2 (en) | 1991-11-04 | 2001-10-22 | アデザ バイオメディカル コーポレイション | Screening method to identify women at increased risk of preterm labor |
WO1993009432A1 (en) | 1991-11-04 | 1993-05-13 | Adeza Biomedical Corporation | Screening method for identifying women at increased risk for imminent delivery |
US5516702A (en) | 1991-11-06 | 1996-05-14 | Adeza Biomedical Corporation | Screening method for identifying women at increased risk for imminent delivery |
US5898005A (en) | 1993-02-24 | 1999-04-27 | Dade Behring Inc. | Rapid detection of analytes with receptors immobilized on soluble submicron particles |
ES2132392T3 (en) | 1993-02-24 | 1999-08-16 | Dade Behring Inc | IMMOBILIZATION OF SPECIFIC LINK TEST REAGENTS. |
ATE226959T1 (en) | 1993-03-23 | 2002-11-15 | Victor Voroteliak | METHOD FOR DETECTING TEARS IN AMNIOMEMBRANES IN PREGNANT MAMMALS |
US5431171A (en) | 1993-06-25 | 1995-07-11 | The Regents Of The University Of California | Monitoring fetal characteristics by radiotelemetric transmission |
EP0716746B1 (en) | 1993-09-03 | 1999-03-17 | Behringwerke Ag | Fluorescent oxygen channeling immunoassays |
US5650394A (en) | 1993-11-04 | 1997-07-22 | Adeza Biomedical | Use of urinastatin-like compounds to prevent premature delivery |
US5597700A (en) | 1994-04-28 | 1997-01-28 | California Research, Llc | Method for detecting free insulin-like growth-factor-binding protein 1 and a test device for detecting the ruptures of fetal membranes using the above method |
US5641636A (en) | 1994-05-20 | 1997-06-24 | University Of Pennsylvania | Method of predicting fetal membrane rupture based on matrix metalloproteinase-9 activity |
US6140099A (en) | 1994-05-20 | 2000-10-31 | The Trustees Of The University Of Pennsylvania | Method of delaying fetal membrane rupture by inhibiting matrix metalloproteinase-9 activity |
EP0812353A2 (en) | 1995-01-26 | 1997-12-17 | Merck Frosst Canada Inc. | Prostaglandin receptor dp |
AU706833B2 (en) | 1995-10-31 | 1999-06-24 | Maruha Nichiro Corporation | A prostaglandin D synthase-specific monoclonal antibody |
US6678669B2 (en) | 1996-02-09 | 2004-01-13 | Adeza Biomedical Corporation | Method for selecting medical and biochemical diagnostic tests using neural network-related applications |
US6120460A (en) | 1996-09-04 | 2000-09-19 | Abreu; Marcio Marc | Method and apparatus for signal acquisition, processing and transmission for evaluation of bodily functions |
US6544193B2 (en) | 1996-09-04 | 2003-04-08 | Marcio Marc Abreu | Noninvasive measurement of chemical substances |
FI963989A (en) | 1996-10-04 | 1998-04-05 | Wallac Oy | Homogeneous methods of determination based on the transmission of luminescence energy |
EP0951298A1 (en) | 1996-12-23 | 1999-10-27 | Cambridge University Technical Services Limited | Diagnosis and treatment of pathological pregnancies |
CA2665139A1 (en) | 1997-03-07 | 1998-09-17 | Mount Sinai Hospital | Methods to diagnose a required regulation of trophoblast invasion |
US6875567B1 (en) | 1997-11-10 | 2005-04-05 | Genentech, Inc. | Method of detecting cardiac hypertrophy through probe hybridization and gene expression analysis |
US6610480B1 (en) | 1997-11-10 | 2003-08-26 | Genentech, Inc. | Treatment and diagnosis of cardiac hypertrophy |
US20020031513A1 (en) | 1997-11-24 | 2002-03-14 | Shamir Leibovitz | Method and pharmaceutical composition for inhibiting premature rapture of fetal membranes, ripening of uterine cervix and preterm labor in mammals |
US6267722B1 (en) | 1998-02-03 | 2001-07-31 | Adeza Biomedical Corporation | Point of care diagnostic systems |
US6394952B1 (en) | 1998-02-03 | 2002-05-28 | Adeza Biomedical Corporation | Point of care diagnostic systems |
US6506789B2 (en) | 1998-06-03 | 2003-01-14 | Shionogi & Co., Ltd. | Methods for the treatment of itching comprising administering PGD2 receptor antagonist |
US6149590A (en) | 1998-07-22 | 2000-11-21 | Smith; Ramada S. | System for identifying premature rupture of membrane during pregnancy |
US6126597A (en) | 1998-07-22 | 2000-10-03 | Smith; Ramada S. | System for identifying premature rupture of membrane during pregnancy |
US20010025140A1 (en) | 1998-07-22 | 2001-09-27 | Torok Brian A. | System for identifying premature rupture of membrane during pregnancy |
US7109044B1 (en) | 1998-09-04 | 2006-09-19 | Maruha Corporation | Method of detection and disease state management for renal diseases |
PL347508A1 (en) | 1998-10-29 | 2002-04-08 | Regents Board Of | Use of thiazolidinediones derivatives for preventing uterine contractions in premature labour or lactation |
JP2000249709A (en) | 1999-02-26 | 2000-09-14 | Maruha Corp | Predicting method for restenosis after coronary blood vessel intervention execution |
US6126616A (en) | 1999-06-21 | 2000-10-03 | Sanyal; Mrinal K. | Collection of biological products from human accessory reproductive organs by absorbent systems |
DE1211513T1 (en) | 1999-08-23 | 2002-11-14 | Bml Inc | METHOD FOR IDENTIFYING SUBSTANCES THAT CAN BIND TO PROSTAGLANDIN D RECEPTORS |
WO2001014556A1 (en) | 1999-08-23 | 2001-03-01 | Dana-Farber Cancer Institute, Inc. | Novel b7-4 molecules and uses therefor |
WO2001039722A2 (en) | 1999-11-30 | 2001-06-07 | Mayo Foundation For Medical Education And Research | B7-h1, a novel immunoregulatory molecule |
FR2809182B1 (en) | 2000-05-18 | 2003-08-15 | Univ Rene Descartes | DETECTION OF IL-6 FOR PREDICTING PREMATURE DELIVERY RISKS |
US20010053876A1 (en) | 2000-06-15 | 2001-12-20 | Torok Brian A. | System for identifying premature rupture of membrane during pregnancy |
US6878522B2 (en) | 2000-07-07 | 2005-04-12 | Baiyong Li | Methods for the identification of compounds useful for the treatment of disease states mediated by prostaglandin D2 |
US7314727B2 (en) | 2000-07-21 | 2008-01-01 | Maruha Corporation | Methods to facilitate diagnosis of normal pressure hydrocephalus |
US6410583B1 (en) | 2000-07-25 | 2002-06-25 | Merck Frosst Canada & Co. | Cyclopentanoindoles, compositions containing such compounds and methods of treatment |
US20020046054A1 (en) | 2000-08-28 | 2002-04-18 | Morand Patrick G. | Use of blood and plasma donor samples and data in the drug discovery process |
US7217725B2 (en) | 2000-09-14 | 2007-05-15 | Allergan, Inc. | Prostaglandin D2 antagonist |
EP1351985A2 (en) | 2000-10-16 | 2003-10-15 | Bayer Aktiengesellschaft | Polynucleotide and polypeptide sequences of human dendritic cell immunoreceptors |
EP2339020B1 (en) | 2000-10-20 | 2017-03-08 | Como Biotech APS | Diagnostic methods based on Pregnancy-associated plasma protein-A (PAAP-A) |
GB0026823D0 (en) | 2000-11-02 | 2000-12-20 | King S College London | Diagnosis of pre-eclampsia |
US7635571B2 (en) | 2000-12-07 | 2009-12-22 | Siemens Healthcare Diagnostics Products Gmbh | Amplified signal in binding assays |
DE60215000T2 (en) | 2001-05-23 | 2007-08-09 | Merck Frosst Canada & Co, Kirkland | Dihydropyrroloil [1,2-a] indole and tetrahydropyridol [1,2-a] indole derivatives as prostaglandin D2 receptor antagonists |
US20040185509A1 (en) | 2001-07-18 | 2004-09-23 | Francois Gervais | Eosinophil prostaglandin d2 receptor assays |
US20040197834A1 (en) | 2001-07-20 | 2004-10-07 | Francois Gervais | Method to increase expression of pgd2 receptors and assays for identifying modulators of prostaglandin d2 receptors |
US20050101841A9 (en) | 2001-12-04 | 2005-05-12 | Kimberly-Clark Worldwide, Inc. | Healthcare networks with biosensors |
EP1540341A4 (en) | 2002-07-01 | 2007-01-10 | Univ Rochester Medical Ct | Method for determination of likelihood of occurrence of preterm labor in pregnant females |
US7335362B2 (en) | 2002-07-19 | 2008-02-26 | Beth Israel Deaconess Medical Center | Methods of treating pre-eclampsia or eclampsia |
CA2922031C (en) | 2002-07-19 | 2020-12-15 | Beth Israel Deaconess Medical Center, Inc. | Methods of diagnosing and treating pre-eclampsia or eclampsia |
US7435419B2 (en) | 2002-07-19 | 2008-10-14 | Beth Israel Deaconess Medical Center | Methods of diagnosing and treating pre-eclampsia or eclampsia |
EA008194B1 (en) | 2002-08-13 | 2007-04-27 | Н-Диа, Инк. | Methods for detecting amniotic fluid in vaginal secretions and devices therefor |
ATE483980T1 (en) | 2002-08-29 | 2010-10-15 | Diagnostic Technologies Ltd | METHOD FOR DIAGNOSING PREGNANCY DISORDERS |
US20050215609A1 (en) | 2002-09-30 | 2005-09-29 | Masaaki Yoshikawa | Novel uses of prostaglandin d2, prostaglandin d2 agonist and prostaglandin d2 antagonist |
AU2003284166A1 (en) | 2002-10-23 | 2004-05-13 | University Of Hawaii | Methods for diagnosing and treating pre-term labor |
US20040100376A1 (en) | 2002-11-26 | 2004-05-27 | Kimberly-Clark Worldwide, Inc. | Healthcare monitoring system |
EP1590664A4 (en) | 2003-02-05 | 2007-05-23 | Ciphergen Biosystems Inc | Non-invasive assessment of intra-aminiotic environment |
EP1590668A2 (en) | 2003-02-06 | 2005-11-02 | Adeza Biomedical Corporation | Screeninig and treatment methods for prevention of preterm delivery |
US8068990B2 (en) | 2003-03-25 | 2011-11-29 | Hologic, Inc. | Diagnosis of intra-uterine infection by proteomic analysis of cervical-vaginal fluids |
US7191068B2 (en) | 2003-03-25 | 2007-03-13 | Proteogenix, Inc. | Proteomic analysis of biological fluids |
US8003765B2 (en) | 2003-04-09 | 2011-08-23 | Stony Brook Anaesthesiology, University Faculty Practice Corporation | Methods, antibodies and kits for detecting cerebrospinal fluid in a sample |
US20040203079A1 (en) | 2003-04-09 | 2004-10-14 | Research Foundation Of The State University Of New York | Methods and kits for detecting cerebrospinal fluid in a sample |
US20070003992A1 (en) | 2003-04-09 | 2007-01-04 | Pentyala Srinivas N | Methods and kits for detecting cerebrospinal fluid in a sample |
WO2004098389A2 (en) | 2003-05-02 | 2004-11-18 | Johns Hopkins University | Devices, systems and methods for bioimpendence measurement of cervical tissue and methods for diagnosis and treatment of human cervix |
JP2006528938A (en) | 2003-05-20 | 2006-12-28 | メルク フロスト カナダ リミテツド | Fluoro-methanesulfonyl substituted cycloalkanoindoles and their use as prostaglandin D2 antagonists |
WO2005007223A2 (en) | 2003-07-16 | 2005-01-27 | Sasha John | Programmable medical drug delivery systems and methods for delivery of multiple fluids and concentrations |
CA2539430C (en) | 2003-09-23 | 2015-03-17 | The General Hospital Corporation | Screening for gestational disorders with sex hormone binding globulin as biomarker |
JP3897117B2 (en) | 2003-09-24 | 2007-03-22 | マルハ株式会社 | Method for determining and predicting the severity of pregnancy toxemia, and for evaluating fetal / placental function in pregnancy toxemia |
US20080233597A1 (en) | 2003-09-26 | 2008-09-25 | Yasuhiko Shiina | Method of Detecting or Differentiating Rheumatoid Arthritis and Method of Determining Stage of Disease or Degree of Dysfunction with Regard to Rheumatoid Arthritis |
US20050131287A1 (en) | 2003-12-16 | 2005-06-16 | Kimberly-Clark Worldwide, Inc. | Detection of premature rupture of the amniotic membrane |
US20060024757A1 (en) | 2004-07-30 | 2006-02-02 | Robert Hussa | Detection of oncofetal fibronectin for selection of concepti |
US20080254479A1 (en) | 2004-08-30 | 2008-10-16 | Cervimark, Llc | Methods and Kits For Predicting Risk For Preterm Labor |
US20080090759A1 (en) | 2004-08-30 | 2008-04-17 | Robert Kokenyesi | Methods and kits for predicting risk for preterm labor |
US7740849B2 (en) | 2004-09-24 | 2010-06-22 | Beth Israel Deaconess Medical Center | Use of compounds that bind soluble endoglin and SFLT-1 for the treatment of pregnancy related hypertensive disorders |
DK1804836T3 (en) | 2004-09-24 | 2011-01-24 | Beth Israel Hospital | Methods for diagnosing and treating pregnancy complications |
GB0422057D0 (en) | 2004-10-05 | 2004-11-03 | Astrazeneca Ab | Novel compounds |
GT200500284A (en) | 2004-10-15 | 2006-03-27 | Aventis Pharma Inc | PYRIMIDINS AS ANTAGONISTS OF PROSTAGLANDINA D2 RECEPTOR |
US7727733B2 (en) | 2004-12-21 | 2010-06-01 | Yale University | Diagnosis of preeclampsia |
CA2528531A1 (en) | 2005-01-06 | 2006-07-06 | Mount Sinai Hospital | Markers of pre-term labor |
DOP2006000016A (en) | 2005-01-26 | 2006-07-31 | Aventis Pharma Inc | 2-PHENYL-INDOLES AS ANTAGONISTS OF THE PROSTAGLANDIN RECEPTOR D2. |
GB0505048D0 (en) | 2005-03-11 | 2005-04-20 | Oxagen Ltd | Compounds with PGD antagonist activity |
JP2009501715A (en) | 2005-07-13 | 2009-01-22 | イーシーアイ バイオテック インコーポレイティッド | Substrates, sensors, and methods for assessing female status |
WO2007010965A1 (en) | 2005-07-22 | 2007-01-25 | Shionogi & Co., Ltd. | Azaindole derivative having pgd2 receptor antagonistic activity |
WO2007030890A1 (en) | 2005-09-15 | 2007-03-22 | Bio Source (Wuhan) Technology Ltd | A marker for prolonged rupture of membranes |
GT200600457A (en) | 2005-10-13 | 2007-04-27 | Aventis Pharma Inc | DIHYDROGEN PHOSPHATE SALT AS ANTAGONIST OF PROSTAGLANDINA D2 RECEPTOR |
US8076315B2 (en) | 2005-10-14 | 2011-12-13 | The Board Of Trustees Of The University Of Illinois | Pharmacological treatments for sleep disorders (apnoe) with prostanoid receptor antagonists |
US20070111326A1 (en) | 2005-11-14 | 2007-05-17 | Abbott Laboratories | Diagnostic method for proteinaceous binding pairs, cardiovascular conditions and preeclampsia |
GB0600916D0 (en) | 2006-01-17 | 2006-02-22 | Perkinelmer Instr Las Inc | detecting and predicting pre-eclampsia |
US20080009552A1 (en) | 2006-03-23 | 2008-01-10 | Craig Pennell | Markers of pre-term labor |
AR060403A1 (en) | 2006-04-12 | 2008-06-11 | Sanofi Aventis | AMINO COMPOUNDS- PYRIMIDINE 2.6- SUBSTITUTES -4- MONOSUSTITUTES AS ANTAGONISTS OF PROSTAGLANDINA D2 RECEIVER |
HUE030850T2 (en) | 2006-06-16 | 2017-06-28 | Univ Pennsylvania | Prostaglandin d2 receptor antagonists for treating androgenetic alopecia |
KR20090039735A (en) | 2006-07-25 | 2009-04-22 | 사노피-아벤티스 | 2-phenyl-indoles as prostaglandin d2 receptor antagonists |
US8133859B2 (en) | 2006-09-27 | 2012-03-13 | The United States Of America As Represented By The Department Of Health And Human Services | SCGB3A2 as a growth factor and anti-apoptotic agent |
EP2918288B1 (en) | 2006-10-03 | 2017-08-16 | Genzyme Corporation | Use of TGF beta antagonists to treat infants at risk of developing bronchopulmonary dysplasia |
WO2008073491A1 (en) | 2006-12-11 | 2008-06-19 | University Of Florida Research Foundation, Inc. | System and method for analyzing progress of labor and preterm labor |
CA2672373C (en) | 2006-12-19 | 2011-08-30 | Pfizer Products Inc. | Nicotinamide derivatives as inhibitors of h-pgds and their use for treating prostaglandin d2 mediated diseases |
US20100136012A1 (en) | 2006-12-20 | 2010-06-03 | Hadasit Medical Research Services And Development Ltd. | Variants of vegfr and their use in the diagnosis and treatment of pregnancy associated medical conditions |
US8552364B2 (en) | 2006-12-26 | 2013-10-08 | Brigham Young University | Serum proteomics system and associated methods |
EP2132196A1 (en) | 2007-02-26 | 2009-12-16 | Pfizer Products Inc. | Nicotinamide derivatives as inhibitors of h-pgds and their use for treating prostaglandin d2 mediated diseases |
US20090104649A1 (en) | 2007-06-11 | 2009-04-23 | Garovic Vesna D | Markers for preeclampsia |
US20090058072A1 (en) | 2007-08-30 | 2009-03-05 | Shirlee Ann Weber | Record sheets with integrated themes |
US20100251394A1 (en) | 2007-09-20 | 2010-09-30 | The Johns Hopkins University | Treatment and prevention of ischemic brain injury |
US20110002866A1 (en) | 2007-10-31 | 2011-01-06 | Lubit Beverly W | Methods to prevent a hair-related side effect of treatment with a chemotherapeutic agent |
WO2009059259A2 (en) | 2007-10-31 | 2009-05-07 | Children's Hospital Medical Center | Detection of worsening renal disease in subjects with systemic lupus erythematosus |
US20100298368A1 (en) | 2007-11-06 | 2010-11-25 | Amira Pharmaceuticals, Inc. | Antagonists of pgd2 receptors |
US20110065139A1 (en) | 2007-11-27 | 2011-03-17 | Jacob Mullerad | diagnostic device for identifying rupture of membrane during pregnancy |
US20130177485A1 (en) | 2007-11-27 | 2013-07-11 | Momtec Life Ltd. Of Yazmot Haemek | Diagnostic device for identifying rupture of membrane during pregnancy |
AU2008337342B2 (en) | 2007-12-14 | 2012-05-24 | Pulmagen Therapeutics (Asthma) Limited | Indoles and their therapeutic use |
US8835183B2 (en) * | 2008-01-07 | 2014-09-16 | Ortho-Clinical Diagnostics, Inc. | Determination of sFlt-1:angiogenic factor complex |
EP2245010A1 (en) | 2008-01-18 | 2010-11-03 | Pulmagen Therapeutics (Asthma) Limited | Indoles active on crth2 receptor |
CA2967508C (en) | 2008-01-25 | 2020-02-25 | The Fetal Medicine Foundation | Methods for determining the risk of prenatal complications |
WO2009097584A1 (en) | 2008-01-30 | 2009-08-06 | Proteogenix, Inc. | Maternal serum biomarkers for detection of pre-eclampsia |
US20100017143A1 (en) | 2008-01-30 | 2010-01-21 | Proteogenix, Inc. | Gestational age dependent proteomic changes of human maternal serum for monitoring maternal and fetal health |
CA2713139C (en) | 2008-02-01 | 2013-04-23 | Amira Pharmaceuticals, Inc. | N,n-disubstituted aminoalkylbiphenyl antagonists of prostaglandin d2 receptors |
GB2460597B8 (en) | 2008-02-01 | 2014-03-12 | Amira Pharmaceuticals Inc | N,N-disubstituted aminoalkylbiphenyl antagonists of prostaglandin D2 receptors |
EP2257536A4 (en) | 2008-02-14 | 2011-03-23 | Amira Pharmaceuticals Inc | Cyclic diaryl ether as antagonists of prostaglandin d2 receptors |
EP2245022A4 (en) | 2008-02-25 | 2012-02-22 | Panmira Pharmaceuticals Llc | Antagonists of prostaglandin d2 receptors |
US8426449B2 (en) | 2008-04-02 | 2013-04-23 | Panmira Pharmaceuticals, Llc | Aminoalkylphenyl antagonists of prostaglandin D2 receptors |
US20110112134A1 (en) | 2008-05-16 | 2011-05-12 | Amira Pharmaceuticals, Inc. | Tricyclic Antagonists of Prostaglandin D2 Receptors |
WO2009140661A1 (en) | 2008-05-16 | 2009-11-19 | Corthera, Inc. | Method of preventing premature delivery |
JP2011526281A (en) | 2008-06-24 | 2011-10-06 | アミラ ファーマシューティカルズ,インク. | Cycloalkane [B] indole antagonist of prostaglandin D2 receptor |
BRPI0913930A2 (en) | 2008-07-03 | 2015-10-20 | Amira Pharmaceuticals Inc | prostaglandin d2 receptor antagonists |
FR2934681B1 (en) | 2008-07-29 | 2011-09-30 | Bastien Karkouche | DEVICE FOR CAPTURING BIOLOGICAL PARTICLES AND USE. |
EP2316034A1 (en) | 2008-08-04 | 2011-05-04 | The Board of Regents of The University of Texas System | Multiplexed diagnostic test for preterm labor |
WO2010033977A2 (en) | 2008-09-22 | 2010-03-25 | Cayman Chemical Company | Multiheteroaryl compounds as inhibitors of h-pgds and their use for treating prostaglandin d2 mediated diseases |
US8552154B2 (en) | 2008-09-26 | 2013-10-08 | Emory University | Anti-PD-L1 antibodies and uses therefor |
GB2463788B (en) | 2008-09-29 | 2010-12-15 | Amira Pharmaceuticals Inc | Heteroaryl antagonists of prostaglandin D2 receptors |
WO2010039977A2 (en) | 2008-10-01 | 2010-04-08 | Amira Pharmaceuticals, Inc. | Heteroaryl antagonists of prostaglandin d2 receptors |
WO2010042652A2 (en) | 2008-10-08 | 2010-04-15 | Amira Pharmaceuticals, Inc. | Heteroalkyl biphenyl antagonists of prostaglandin d2 receptors |
GB2465062B (en) | 2008-11-06 | 2011-04-13 | Amira Pharmaceuticals Inc | Cycloalkane(B)azaindole antagonists of prostaglandin D2 receptors |
US8383654B2 (en) | 2008-11-17 | 2013-02-26 | Panmira Pharmaceuticals, Llc | Heterocyclic antagonists of prostaglandin D2 receptors |
US9180114B2 (en) | 2008-11-26 | 2015-11-10 | President And Fellows Of Harvard College | Neurodegenerative diseases and methods of modeling |
WO2010085820A2 (en) | 2009-01-26 | 2010-07-29 | Amira Pharmaceuticals, Inc. | Tricyclic compounds as antagonists of prostaglandin d2 receptors |
US20120046261A1 (en) | 2009-02-27 | 2012-02-23 | University Of Utah Research Foundation | Compositions and methods for diagnosing and preventing spontaneous preterm birth |
GB0905964D0 (en) | 2009-04-06 | 2009-05-20 | King S College London | Marker |
AU2010240781B2 (en) | 2009-04-23 | 2015-05-28 | Wallac Oy | Methods for determining maternal health risks |
CN102596902A (en) | 2009-08-05 | 2012-07-18 | 潘米拉制药公司 | DP2 antagonist and uses thereof |
US20110090048A1 (en) | 2009-09-29 | 2011-04-21 | Li Conan K | Data Transmission Device with User Identification Capability |
CA2779270A1 (en) | 2009-10-29 | 2011-05-05 | The Trustees Of The University Of Pennsylvania | Method of predicting risk of preterm birth |
RU2556524C2 (en) | 2009-11-12 | 2015-07-10 | Сервиленц Инк. | Devices and methods for measuring cervix of uterus |
CN102667486B (en) | 2009-11-25 | 2016-03-09 | 霍洛吉克股份有限公司 | The detection of IAI |
US8518716B2 (en) | 2009-11-30 | 2013-08-27 | University Of Pittsburgh-Of The Commonwealth System Of Higher Education | Soluble CD117 (sc-kit) for diagnosis of preeclampsia and eclampsia |
US20120238469A1 (en) | 2009-12-08 | 2012-09-20 | Cedars-Sinai Medical Center | Diagnostic biomarker to identify women at risk for preterm delivery |
US8874183B2 (en) | 2010-02-18 | 2014-10-28 | The Johns Hopkins University | Preterm labor monitor |
EP2547672A1 (en) | 2010-03-16 | 2013-01-23 | Aventis Pharmaceuticals Inc. | Substituted pyrimidines as prostaglandin d2 receptor antagonists |
MX2012010038A (en) | 2010-03-16 | 2012-10-01 | Aventis Pharma Inc | A substituted pyrimidine as a prostaglandin d2 receptor antagonist. |
MA34094B1 (en) | 2010-03-22 | 2013-03-05 | Actelion Pharmaceuticals Ltd | 3- (HETEROARYLAMINO) -1,2,3,4-TETRAHYDRO-9H-CARBAZOLE DERIVATIVES AND THEIR USE AS MODULATORS OF THE PROSTAGLANDIN D2 RECEPTOR |
US20110237972A1 (en) | 2010-03-23 | 2011-09-29 | Robert Garfield | Noninvasive measurement of uterine emg propagation and power spectrum frequency to predict true preterm labor and delivery |
JP2013524251A (en) | 2010-04-13 | 2013-06-17 | プロノタ エヌ.ヴェ. | Biomarkers for pregnancy-induced hypertension |
EP2385134A1 (en) | 2010-05-07 | 2011-11-09 | Ludwig-Maximilians-Universität München | Risk prognosis method for chronic lymphocytic leukemia |
CN102884006B (en) | 2010-05-14 | 2016-02-17 | 贝斯以色列护理医疗中心 | The external device for the treatment of pregnancy complications and method |
US20110312928A1 (en) | 2010-06-18 | 2011-12-22 | Lipocine Inc. | Progesterone Containing Oral Dosage Forms and Related Methods |
US20110312927A1 (en) | 2010-06-18 | 2011-12-22 | Satish Kumar Nachaegari | Progesterone Containing Oral Dosage Forms and Related Methods |
WO2011162821A1 (en) | 2010-06-23 | 2011-12-29 | Astute Medical, Inc. | Methods and compositions for diagnosis and prognosis of renal injury and renal failure |
US20110318308A1 (en) | 2010-06-25 | 2011-12-29 | Winthrop-University Hospital | Lipocalin-type prostaglandin d2 synthase as a biomarker for lung cancer progression and prognosis |
AU2011275417A1 (en) | 2010-07-05 | 2013-02-21 | Actelion Pharmaceuticals Ltd | 1-phenyl-substituted heterocyclyl derivatives and their use as prostaglandin D2 receptor modulators |
AP2013006667A0 (en) | 2010-07-12 | 2013-01-31 | Therasyn Sensors Inc | A device and methods for in vivo monitoring of an individual |
US8765487B2 (en) | 2010-09-01 | 2014-07-01 | Clinical Innovations | Detection of amniotic fluid in vaginal secretions of pregnant women due to premature rupture of fetal membranes |
AU2011308615B2 (en) | 2010-10-01 | 2015-02-05 | Hologic, Inc. | Immunoassay test strip for use in a diagnostic system |
WO2012049316A1 (en) | 2010-10-15 | 2012-04-19 | Olink Ab | Dynamic range methods |
EP2649454B1 (en) | 2010-12-06 | 2018-06-20 | Mycartis N.V. | Biomarkers and parameters for hypertensive disorders of pregnancy |
SG192226A1 (en) | 2011-01-31 | 2013-09-30 | Esoterix Genetic Lab Llc | Methods for enriching microparticles or nucleic acids using binding molecules |
GB201101621D0 (en) | 2011-01-31 | 2011-03-16 | Olink Ab | Method and product |
US8951996B2 (en) | 2011-07-28 | 2015-02-10 | Lipocine Inc. | 17-hydroxyprogesterone ester-containing oral compositions and related methods |
ES2650263T3 (en) | 2011-11-09 | 2018-01-17 | F. Hoffmann-La Roche Ag | Dynamics of the ratio of SFLT-1 or endogline / PLGF as an indicator for impending preeclampsia and / or HELLP syndrome |
CA2859295A1 (en) | 2011-12-15 | 2013-06-20 | Pronota N.V. | Biomarkers and parameters for preeclampsia |
US20130203068A1 (en) | 2012-02-06 | 2013-08-08 | Perkinelmer Biosignal, Inc. | Dual-acceptor time-resolved-fret |
GB2515983A (en) | 2012-05-08 | 2015-01-07 | Univ Leland Stanford Junior | Methods and compositions for providing a preeclampsia assessment |
EP3339861B8 (en) | 2012-06-15 | 2023-11-01 | Genesis Theranostix Korlatolt Felelossegu Tarsasag | Biomarker test for prediction or early detection of preeclampsia |
KR20150023063A (en) | 2012-06-27 | 2015-03-04 | 에프. 호프만-라 로슈 아게 | MEANS AND METHODS APPLYING sFlt-1/PlGF OR ENDOGLIN/PlGF RATIO TO RULE-OUT ONSET OF PREECLAMPSIA WITHIN A CERTAIN TIME PERIOD |
WO2014036440A2 (en) | 2012-08-30 | 2014-03-06 | Ansh Labs Llc | Papp-a2 as a marker for monitoring, predicting and diagnosing preeclampsia |
EP2912458B1 (en) | 2012-10-24 | 2018-07-18 | NYU Winthrop Hospital | Non-invasive biomarker to identify subjects at risk of preterm delivery |
WO2014078622A1 (en) | 2012-11-15 | 2014-05-22 | The Brigham And Women's Hospital, Inc. | Method and system for diagnosing and treating preeclampsia |
EP2965088B1 (en) | 2013-03-04 | 2018-12-05 | IQ Products B.V. | Prognostic marker to determine the risk for early-onset preeclampsia |
WO2014142752A1 (en) | 2013-03-12 | 2014-09-18 | Agency For Science, Technology And Research | Pre-eclampsia biomarkers |
US20140273025A1 (en) | 2013-03-15 | 2014-09-18 | Wallac Oy | System and method for determining risk of pre-eclampsia based on biochemical marker analysis |
EP3077825B1 (en) | 2013-12-03 | 2020-11-25 | Cézanne S.A.S. | Method for the selective determination of placental growth factor 2 |
WO2015110624A1 (en) | 2014-01-24 | 2015-07-30 | Roche Diagnostics Gmbh | Prediction of postpartum hellp syndrome, postpartum eclampsia or postpartum preeclampsia |
WO2015148989A1 (en) | 2014-03-27 | 2015-10-01 | Wayne State University | Systems and methods to identify and treat subjects at risk for obstetrical complications |
CA2956646A1 (en) | 2014-07-30 | 2016-02-04 | Matthew Cooper | Methods and compositions for diagnosing, prognosing, and confirming preeclampsia |
EP3189336B1 (en) | 2014-09-02 | 2018-11-07 | Wallac OY | Method for determining risk of pre-eclampsia |
WO2016132136A1 (en) | 2015-02-18 | 2016-08-25 | Aston University | Diagnostic assay and treatment for preeclampsia |
WO2016134324A1 (en) | 2015-02-20 | 2016-08-25 | Neoteryx, Llc | Method and apparatus for acquiring blood for testing |
US20180114600A1 (en) | 2015-03-23 | 2018-04-26 | Adelaide Research & Innovation Pty Ltd | Methods and systems for determining risk of a pregnancy complication occurring |
CA2998275A1 (en) | 2015-09-11 | 2017-03-16 | Universidad De Los Andes | In vitro method for identifying a pregnancy related disease |
NL2016967B1 (en) | 2016-06-15 | 2017-12-21 | Iq Products B V | Markers and their ratio to determine the risk for early-onset preeclampsia. |
US20190353667A1 (en) | 2017-02-06 | 2019-11-21 | Astute Medical, Inc. | Methods and Compositions for Diagnosis and Prognosis of Renal Injury and Renal Failure |
CN111094988A (en) | 2017-09-13 | 2020-05-01 | 普罗根尼蒂公司 | Pre-eclampsia biomarkers and related systems and methods |
US20190079097A1 (en) | 2017-09-13 | 2019-03-14 | Progenity, Inc. | Preeclampsia biomarkers and related systems and methods |
EP4070113A4 (en) | 2019-12-04 | 2023-12-20 | Biora Therapeutics, Inc. | Assessment of preeclampsia using assays for free and dissociated placental growth factor |
WO2021113710A1 (en) | 2019-12-04 | 2021-06-10 | Progenity, Inc. | Assessment of preeclampsia using assays for free and dissociated placental growth factor |
-
2020
- 2020-12-04 EP EP20895054.3A patent/EP4070113A4/en active Pending
- 2020-12-11 US US17/119,410 patent/US11112403B2/en active Active
-
2021
- 2021-08-04 US US17/393,708 patent/US11327071B2/en active Active
-
2022
- 2022-03-31 US US17/710,756 patent/US20230052697A1/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20080206889A1 (en) * | 2007-02-26 | 2008-08-28 | Response Biomedical Corporation | Comparative multiple analyte assay |
WO2009155324A2 (en) * | 2008-06-18 | 2009-12-23 | Abbott Laboratories | Pigf-1 assay and kits and components thereof |
Non-Patent Citations (3)
Title |
---|
Bychkova et al., Fold Des. 1998;3(4):285-91 (Year: 1998) * |
Muccio et al., Biochemistry 1992, 31, 5560-5567 (Year: 1992) * |
Rao and Prakash, JBC, 1993; 20: 14769-14775 (Year: 1993) * |
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