US20200115354A1 - Therapeutic compounds and methods of use thereof - Google Patents

Therapeutic compounds and methods of use thereof Download PDF

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Publication number
US20200115354A1
US20200115354A1 US16/576,569 US201916576569A US2020115354A1 US 20200115354 A1 US20200115354 A1 US 20200115354A1 US 201916576569 A US201916576569 A US 201916576569A US 2020115354 A1 US2020115354 A1 US 2020115354A1
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alkyl
compound
amino
benzenesulfonamide
chloro
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US16/576,569
Inventor
Daniel Sutherlin
Steven MCKERRALL
Michael Scott Wilson
Kwong Wah Lai
Philippe Bergeron
Birong Zhang
Ramsay Beveridge
Jean-Philippe Leclerc
Alexandre Lemire
Liang Zhao
Claudio Sturino
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Xenon Pharmaceuticals Inc
Genentech Inc
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Xenon Pharmaceuticals Inc
Genentech Inc
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Priority to US16/576,569 priority Critical patent/US20200115354A1/en
Publication of US20200115354A1 publication Critical patent/US20200115354A1/en
Abandoned legal-status Critical Current

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    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
    • A61K31/425—Thiazoles
    • A61K31/426—1,3-Thiazoles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
    • A61K31/425—Thiazoles
    • A61K31/427—Thiazoles not condensed and containing further heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
    • A61K31/433—Thidiazoles
    • A—HUMAN NECESSITIES
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    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/4427—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
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    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/506—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim not condensed and containing further heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/535—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one oxygen as the ring hetero atoms, e.g. 1,2-oxazines
    • A61K31/5355—Non-condensed oxazines and containing further heterocyclic rings
    • A—HUMAN NECESSITIES
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    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
    • C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
    • C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D213/72—Nitrogen atoms
    • C07D213/76—Nitrogen atoms to which a second hetero atom is attached
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
    • C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
    • C07D239/24—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
    • C07D239/28—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to ring carbon atoms
    • C07D239/69—Benzenesulfonamido-pyrimidines
    • C—CHEMISTRY; METALLURGY
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    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D261/00—Heterocyclic compounds containing 1,2-oxazole or hydrogenated 1,2-oxazole rings
    • C07D261/02—Heterocyclic compounds containing 1,2-oxazole or hydrogenated 1,2-oxazole rings not condensed with other rings
    • C07D261/06—Heterocyclic compounds containing 1,2-oxazole or hydrogenated 1,2-oxazole rings not condensed with other rings having two or more double bonds between ring members or between ring members and non-ring members
    • C07D261/10—Heterocyclic compounds containing 1,2-oxazole or hydrogenated 1,2-oxazole rings not condensed with other rings having two or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D261/14—Nitrogen atoms
    • C07D261/16—Benzene-sulfonamido isoxazoles
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
    • C07D277/02—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings
    • C07D277/20—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D277/32—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D277/38—Nitrogen atoms
    • C07D277/50—Nitrogen atoms bound to hetero atoms
    • C07D277/52—Nitrogen atoms bound to hetero atoms to sulfur atoms, e.g. sulfonamides
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    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D285/00—Heterocyclic compounds containing rings having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by groups C07D275/00 - C07D283/00
    • C07D285/01—Five-membered rings
    • C07D285/02—Thiadiazoles; Hydrogenated thiadiazoles
    • C07D285/04—Thiadiazoles; Hydrogenated thiadiazoles not condensed with other rings
    • C07D285/08—1,2,4-Thiadiazoles; Hydrogenated 1,2,4-thiadiazoles
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    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
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    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
    • C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
    • C07D403/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
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    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
    • C07D417/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings linked by a chain containing hetero atoms as chain links

Definitions

  • the present invention relates to organic compounds useful for therapy in a mammal, and in particular to inhibitors of sodium channel (e.g., NaV1.7) that are useful for treating sodium channel-mediated diseases or conditions, such as pain, as well as other diseases and conditions associated with the modulation of sodium channels.
  • sodium channel-mediated diseases or conditions such as pain, as well as other diseases and conditions associated with the modulation of sodium channels.
  • Voltage-gated sodium channels are transmembrane proteins that initiate action potentials in nerve, muscle and other electrically excitable cells, and are a necessary component of normal sensation, emotions, thoughts and movements (Catterall, W. A., Nature (2001), Vol. 409, pp. 988-990). These channels consist of a highly processed alpha subunit that is associated with auxiliary beta subunits. The pore-forming alpha subunit is sufficient for channel function, but the kinetics and voltage dependence of channel gating are in part modified by the beta subunits (Goldin et al., Neuron (2000), Vol. 28, pp. 365-368).
  • Electrophysiological recording, biochemical purification, and molecular cloning have identified ten different sodium channel alpha subunits and four beta subunits (Yu, F. H., et al., Sci. STKE (2004), 253; and Yu, F. H., et al., Neurosci. (2003), 20:7577-85).
  • the sodium channel family of proteins has been extensively studied and shown to be involved in a number of vital body functions. Research in this area has identified variants of the alpha subunits that result in major changes in channel function and activities, which can ultimately lead to major pathophysiological conditions.
  • the members of this family of proteins are denoted NaV1.1 to NaV1.9.
  • NaV1.7 is a tetrodotoxin-sensitive voltage-gated sodium channel encoded by the gene SCN9A.
  • Human NaV1.7 was first cloned from neuroendocrine cells (Klugbauer, N., et al., 1995 EMBO J., 14 (6): 1084-90.) and rat NaV1.7 was cloned from a pheochromocytoma PC12 cell line (Toledo-Aral, J. J., et al., Proc. Natl. Acad. Sci. USA (1997), 94:1527-1532) and from rat dorsal root ganglia (Sangameswaran, L., et al., (1997), J. Biol.
  • NaV1.7 is expressed primarily in the peripheral nervous system, especially nocieptors and olfactory neurons and sympathetic neurons. The inhibition, or blocking, of NaV1.7 has been shown to result in analgesic activity. Knockout of NaV1.7 expression in a subset of sensory neurons that are predominantly nociceptive results in resistance to inflammatory pain (Nassar, et al., op. cit.). Likewise, loss of function mutations in humans results in congenital indifference to pain (CIP), in which the individuals are resistant to both inflammatory and neuropathic pain (Cox, J. J., et al., Nature (2006); 444:894-898; Goldberg, Y.
  • CIP congenital indifference to pain
  • the present invention provides novel compounds having sodium channel blocking activity that are useful for the treatment of pain.
  • R 4 and Z together with the benzene ring to which they are both attached form a 9- or 10-membered heterobicycle, or a pharmaceutically acceptable salt thereof.
  • E6 The compound of E1, E2, E3, E4, or E5, wherein one of Y 1 and Y 2 is —CH(R 8 )— and the other of Y 1 and Y 2 is —CH 2 —; R 8 is not H; Y 3 and Y 6 are each —CH 2 —; and Y 4 and Y 5 are each —CH—; or a pharmaceutically acceptable salt thereof.
  • E12 The compound of E6, E7, E8, E9, E10, or E11, wherein R 8 is trifluoromethyl or phenyl that is optionally substituted with one to three substituents each independently selected from C 1 -C 6 alkyl, cyano, halo, and C 1 -C 6 haloalkyl; or a pharmaceutically acceptable salt thereof.
  • R 8 is phenyl that is optionally substituted with one to three substituents R X each independently selected from the group consisting of C 1 -C 6 alkyl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkoxy, C 1 -C 6 haloalkoxy, cyano, halo, hydroxy, C 3 -C 6 cycloalkoxy, C 3 -C 6 halocycloalkyl, —S(C 1 -C 6 alkyl), —S(O)(C 1 -C 6 alkyl), —S(O) 2 (C 1 -C 6 alkyl), —NH 2 —NH(C 1 -C 6 alkyl), —N(C 1 -C 6 alkyl) 2 , 4-6 membered heterocycle, and C 1 -C 6 haloalkyl; or a pharmaceutically acceptable salt
  • E15 The compound of E6, E7, E8, E9, E10, or E11, wherein R 8 is phenyl that is optionally substituted with one to three substituents R X each independently selected from the group consisting of C 1 -C 6 alkyl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkoxy, C 1 -C 6 haloalkoxy, cyano, halo, and C 1 -C 6 haloalkyl; or a pharmaceutically acceptable salt thereof.
  • E16 The compound of E6, E7, E8, E9, E10, or E11, wherein R 8 is phenyl that is optionally substituted with one to three substituents R X each independently selected from the group consisting of F, Cl, CN, C 1 -C 6 alkyl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkoxy, C 1 -C 6 haloalkyl, and C 1 -C 6 haloalkoxy; or a pharmaceutically acceptable salt thereof.
  • E17 The compound of E6, E7, E8, E9, E10, or E11, wherein R 8 is phenyl that is optionally substituted with one to three substituents R X each independently selected from the group consisting of fluoro, chloro, trifluoromethyl, propyl, cyclopropyl, and trifluoromethoxy; or a pharmaceutically acceptable salt thereof.
  • each R X is independently selected from the group consisting of C 1 -C 6 alkyl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkoxy, C 1 -C 6 haloalkoxy, cyano, halo, and C 1 -C 6 haloalkyl; or a pharmaceutically acceptable salt thereof.
  • each R X is independently selected from the group consisting of F, Cl, CN, C 1 -C 6 alkyl, C 3 -C 6 cycloalkyl, C 1 -C 6 alkoxy, C 1 -C 6 haloalkyl, and C 1 -C 6 haloalkoxy; or a pharmaceutically acceptable salt thereof.
  • each R X is independently selected from the group consisting of fluoro, chloro, trifluoromethyl, propyl, cyclopropyl, and trifluoromethoxy; or a pharmaceutically acceptable salt thereof.
  • E27 The compound of E18, or E19, E20, E21, E22, or E23, wherein each R X is independently selected from F and CF 3 ; or a pharmaceutically acceptable salt thereof.
  • E28 The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, or E17, wherein X is —N(R 6 ) 2 and each R 6 is independently selected from hydrogen, C 2 -C 6 alkoxyalkyl, C 1 -C 6 alkyl, benzyl, C 3 -C 6 cycloalkyl, C 1 -C 6 haloalkyl, and C 1 -C 6 hydroxyalkyl; or a pharmaceutically acceptable salt thereof.
  • E29 The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, or E17, wherein X is —N(R 6 ) 2 and two R 6 groups together with the nitrogen to which they are both attached form a 4- to 7-membered heterocycle; or a pharmaceutically acceptable salt thereof.
  • E30 The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, or E17, wherein X is selected from the group consisting of: —NH 2 , —NHMe, —N(Me) 2 , —NHEt, —NMeEt, —N+(Me) 3 ,
  • E31 The compound of E1, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, or E17, wherein X is selected from the group consisting of:
  • E32 The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, or E31, wherein R is H; or a pharmaceutically acceptable salt thereof.
  • E33 The compound of The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, or E32, wherein R 2 is selected from hydrogen and halo; or a pharmaceutically acceptable salt thereof.
  • E34 The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, or E32, wherein R 2 is selected from hydrogen and fluoro; or a pharmaceutically acceptable salt thereof.
  • E35 The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, E32, E33, or E34, wherein R 3 is hydrogen; or a pharmaceutically acceptable salt thereof.
  • E36 The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, E32, E33, E34, or E35, wherein R 4 is selected from fluoro, chloro, bromo, cyclopropyl, and trifluoromethyl; or a pharmaceutically acceptable salt thereof.
  • E37 The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, E32, E33, E34, or E35, wherein R 4 is selected from hydrogen, fluoro, chloro, bromo, cyclopropyl, cyclobutyl, hydroxy, methoxy, and trifluoromethyl; or a pharmaceutically acceptable salt thereof.
  • E38 The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, E32, E33, E34, or E35, wherein R 4 is chloro; or a pharmaceutically acceptable salt thereof.
  • E39 The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, E32, E33, E34, E35, E36, E37, or E38, wherein Het is a 5-membered heteroaryl optionally substituted with one to three substituents each independently selected from C 1 -C 6 alkyl, cyano, halo, and C 1 -C 6 haloalkyl; or a pharmaceutically acceptable salt thereof.
  • E40 The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, E32, E33, E34, E35, E36, E37, or E38, wherein Het is a 6-membered heteroaryl optionally substituted with one to three substituents each independently selected from C 1 -C 6 alkyl, cyano, halo, and C 1 -C 6 haloalkyl; or a pharmaceutically acceptable salt thereof.
  • E41 The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, E32, E33, E34, E35, E36, E37, or E38, wherein Het is selected from the group consisting of: thiazole, pyrimidine, oxazole, and thiadiazole, each optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of C 1 -C 6 alkyl, cyano, halo, and C 1 -C 6 haloalkyl; or a pharmaceutically acceptable salt thereof.
  • Het is selected from the group consisting of: thiazole, pyrimidine, oxazole, and thiadiazole, each optionally substituted with 1,
  • E42 The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, E32, E33, E34, E35, E36, E37, or E38, wherein Het is selected from the group consisting of: 1,3-thiazol-2-yl, pyrimidin-4-yl, e, 1,2-oxazol-3-yl, and 1,2,4-thiadiazol-5-yl; or a pharmaceutically acceptable salt thereof.
  • E45 The compound of E1, which is selected from the group consisting of:
  • E46 The compound of E1, which is selected from the group consisting of:
  • E47 The compound of E1, which is selected from the group consisting of:
  • E53 A compound of formula of E1 or E2 selected from:
  • E54 A compound of E1 or E2 selected from:
  • E55 A compound of E1 or E2 selected from:
  • E56 The compound of E1, E2, E3, E4, E5, E28, E29, E30, E31, E32, E33, E34, E35, E36, E37, E38, E39, E40, E41, or E42, wherein R 8 or R 9 taken together with another R 8 or R 9 and the atoms to which they are attached form a 3-8 membered fused ring, which is a benzo ring.
  • the present invention provides for a pharmaceutical composition
  • a pharmaceutical composition comprising a compound of formula I or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.
  • the present invention provides for a method of treating a disease or condition in a mammal selected from the group consisting of pain, depression, cardiovascular diseases, respiratory diseases, and psychiatric diseases, and combinations thereof, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount of a compound of formula I, or a pharmaceutically acceptable salt thereof.
  • said disease or condition is selected from the group consisting of neuropathic pain, inflammatory pain, visceral pain, cancer pain, chemotherapy pain, trauma pain, surgical pain, post-surgical pain, childbirth pain, labor pain, neurogenic bladder, ulcerative colitis, chronic pain, persistent pain, peripherally mediated pain, centrally mediated pain, chronic headache, migraine headache, sinus headache, tension headache, phantom limb pain, dental pain, peripheral nerve injury or a combination thereof.
  • said disease or condition is selected from the group consisting of pain associated with HIV, HIV treatment induced neuropathy, trigeminal neuralgia, post-herpetic neuralgia, eudynia, heat sensitivity, tosarcoidosis, irritable bowel syndrome, Crohns disease, pain associated with multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS), diabetic neuropathy, peripheral neuropathy, arthritis, rheumatoid arthritis, osteoarthritis, atherosclerosis, paroxysmal dystonia, myasthenia syndromes, myotonia, malignant hyperthermia, cystic fibrosis, pseudoaldosteronism, rhabdomyolysis, hypothyroidism, bipolar depression, anxiety, schizophrenia, sodium channel toxin related illnesses, familial erythromelalgia, primary erythromelalgia, familial rectal pain, cancer, epilepsy, partial and general tonic seizures, restless leg syndrome, ar
  • the present invention provides for a method of treating pain in a mammal by the inhibition of ion flux through a voltage-dependent sodium channel in the mammal, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount of a compound of formula I, or a pharmaceutically acceptable salt thereof.
  • the present invention provides for a method of decreasing ion flux through a voltage-dependent sodium channel in a cell in a mammal, wherein the method comprises contacting the cell with a compound of formula I, or a pharmaceutically acceptable salt thereof.
  • the present invention provides for a method of treating pruritus in a mammal, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount of a compound of formula I, or a pharmaceutically acceptable salt thereof.
  • the present invention provides for a method of treating cancer in a mammal, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount a compound of formula I, or a pharmaceutically acceptable salt thereof.
  • the present invention provides for a method of treating, but not preventing, pain in a mammal, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount of a compound of formula I, or a pharmaceutically acceptable salt thereof.
  • the pain is selected from the group consisting of neuropathic pain, inflammatory pain, visceral pain, cancer pain, chemotherapy pain, trauma pain, surgical pain, post-surgical pain, childbirth pain, labor pain, neurogenic bladder, ulcerative colitis, chronic pain, persistent pain, peripherally mediated pain, centrally mediated pain, chronic headache, migraine headache, sinus headache, tension headache, phantom limb pain, dental pain, peripheral nerve injury or a combination thereof.
  • the pain is associated with a disease or condition selected from the group consisting of HIV, HIV treatment induced neuropathy, trigeminal neuralgia, post-herpetic neuralgia, eudynia, heat sensitivity, tosarcoidosis, irritable bowel syndrome, Crohns disease, pain associated with multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS), diabetic neuropathy, peripheral neuropathy, arthritis, rheumatoid arthritis, osteoarthritis, atherosclerosis, paroxysmal dystonia, myasthenia syndromes, myotonia, malignant hyperthermia, cystic fibrosis, pseudoaldosteronism, rhabdomyolysis, hypothyroidism, bipolar depression, anxiety, schizophrenia, sodium channel toxin related illnesses, familial erythromelalgia, primary erythromelalgia, familial rectal pain, cancer, epilepsy, partial and general tonic seizures, restless leg syndrome,
  • the present invention provides for a method for the treatment or prophylaxis of pain, depression, cardiovascular disease, respiratory disease, or psychiatric disease, or a combinations thereof, in an animal which method comprises administering an effective amount of a compound of formula I, or a pharmaceutically acceptable salt thereof.
  • the present invention provides for a compound of formula I, or a pharmaceutically acceptable salt thereof for the use as a medicament for the treatment of diseases and disorders selected from the group consisting of pain, depression, cardiovascular diseases, respiratory diseases, and psychiatric diseases, or a combination thereof.
  • the present invention provides for the use of a compound of formula I, or a pharmaceutically acceptable salt thereof for the manufacture of a medicament for the treatment of diseases and disorders selected from the group consisting of pain, depression, cardiovascular diseases, respiratory diseases, and psychiatric diseases, or a combination thereof.
  • the present invention provides for the invention as described herein.
  • alkyl by itself or as part of another substituent, means, unless otherwise stated, a straight or branched chain hydrocarbon radical, having the number of carbon atoms designated (i.e., C 1-8 means one to eight carbons).
  • alkyl groups include methyl, ethyl, n-propyl, iso-propyl, n-butyl, t-butyl, iso-butyl, sec-butyl, n-pentyl, n-hexyl, n-heptyl, n-octyl, and the like.
  • alkoxy alkylamino and “alkylthio”, are used in their conventional sense, and refer to those alkyl groups attached to the remainder of the molecule via an oxygen atom (“oxy”), an amino group (“amino”) or thio group. Additionally, for dialkylamino groups, the alkyl portions can be the same or different.
  • halo by itself or as part of another substituent, mean, unless otherwise stated, a fluorine, chlorine, bromine, or iodine atom.
  • haloalkyl refers to an alkyl that is substituted with one or more (e.g. 1, 2, 3, 4, 5, or 6) halo groups.
  • the term includes an alkyl group having 1-6 carbon atoms that is substituted with one or more halo groups.
  • C 1 -C 6 haloalkyl include fluoromethyl, difluoromethyl, trifluoromethyl, chloromethyl, and 2,2,2-trifluoroethyl.
  • halocycloalkyl refers to a cycloalkyl that is substituted with one or more (e.g. 1, 2, 3, 4, 5, or 6) halo groups.
  • the term includes a cycloalkyl group having 3-6 carbon atoms that is substituted with one or more halo groups.
  • Non-limiting examples of the term C 1 -C 6 halocycloalkyl include 1-fluorocyclopropyl.
  • hydroxyalkyl refers to an alkyl that is substituted with one or more (e.g. 1, 2, 3, 4, 5, or 6) hydroxy groups.
  • the term includes C 1 -C 6 hydroxyalkyl, which is an alkyl group having 1-6 carbon atoms that is substituted with one or more hydroxy groups.
  • Non-limiting examples of the term C 1 -C 6 hydroxyalkyl include hydroxymethyl, 2-hydroxyethyl, and 3-hydroxypropyl.
  • alkoxyalkyl refers to an alkyl that is substituted with one or more (e.g. 1, 2, 3, 4, 5, or 6) alkoxy (alkyl-O—) groups.
  • C 2 -C 6 alkoxyalkyl refers to an alkyl that is substituted with one or more alkoxy groups, wherein the total number of carbons is 2, 3, 4, 5, or 6.
  • Non-limiting examples of the term C 2 -C 6 alkoxyalkyl include methoxymethyl, 2-methoxyethyl, 2-ethoxyethyl, 3-methoxypropyl, and 3-ethoxypropyl.
  • aryl refers to a single all carbon aromatic ring or a multiple condensed all carbon ring system wherein at least one of the rings is aromatic.
  • an aryl group has 6 to 20 carbon atoms, 6 to 14 carbon atoms, or 6 to 12 carbon atoms.
  • Aryl includes a phenyl radical.
  • Aryl also includes multiple condensed ring systems (e.g., ring systems comprising 2, 3 or 4 rings) having about 9 to 20 carbon atoms in which at least one ring is aromatic and wherein the other rings may be aromatic or not aromatic (i.e., carbocycle).
  • Such multiple condensed ring systems are optionally substituted with one or more (e.g., 1, 2 or 3) oxo groups on any carbocycle portion of the multiple condensed ring system.
  • the rings of the multiple condensed ring system can be connected to each other via fused, spiro and bridged bonds when allowed by valency requirements. It is to be understood that the point of attachment of a multiple condensed ring system, as defined above, can be at any position of the ring system including an aromatic or a carbocycle portion of the ring.
  • aryl groups include, but are not limited to, phenyl, indenyl, naphthyl, 1,2,3,4-tetrahydronaphthyl, anthracenyl, and the like.
  • carrier or “carbocyclyl” refers to a single saturated (i.e., cycloalkyl) or a single partially unsaturated (e.g., cycloalkenyl, cycloalkadienyl, etc.) all carbon ring having 3 to 7 carbon atoms (i.e., (C 3 -C 7 )carbocycle).
  • carrier or “carbocyclyl” also includes multiple condensed, saturated and partially unsaturated all carbon ring systems (e.g., ring systems comprising 2, 3 or 4 carbocyclic rings).
  • carbocycle includes multicyclic carbocyles such as a bicyclic carbocycles (e.g., bicyclic carbocycles having about 6 to 20 or 6 to 12 carbon atoms such as bicyclo[3.1.0]hexane and bicyclo[2.1.1]hexane), and polycyclic carbocycles (e.g tricyclic and tetracyclic carbocycles with up to about 20 carbon atoms).
  • the rings of the multiple condensed ring system can be connected to each other via fused, spiro and bridged bonds when allowed by valency requirements.
  • multicyclic carbocyles can be connected to each other via a single carbon atom to form a spiro connection (e.g., spiropentane, spiro[4,5]decane, etc), via two adjacent carbon atoms to form a fused connection (e.g., carbocycles such as decahydronaphthalene, norsabinane, norcarane) or via two non-adjacent carbon atoms to form a bridged connection (e.g., norbornane, bicyclo[2.2.2]octane, etc).
  • a spiro connection e.g., spiropentane, spiro[4,5]decane, etc
  • a fused connection e.g., carbocycles such as decahydronaphthalene, norsabinane, norcarane
  • a bridged connection e.g., norbornane, bicyclo[2.2.2]octane,
  • carbocycle or “carbocyclyl” can also be optionally substituted with one or more (e.g., 1, 2 or 3) oxo groups.
  • carbocycle includes a C 3-12 carbocycle.
  • carbocycle includes a C 3-8 carbocycle.
  • carbocycle includes a C 3-6 carbocycle.
  • carbocycle includes a C 3-5 carbocycle.
  • Non-limiting examples of carbocycles include cyclopropyl, cyclobutyl, cyclopentyl, 1-cyclopent-1-enyl, 1-cyclopent-2-enyl, 1-cyclopent-3-enyl, cyclohexyl, 1-cyclohex-1-enyl, 1-cyclohex-2-enyl, bicyclo[2.2.1]heptane, pinane, adamantane, norborene, spirocyclic C 5-12 alkane, and 1-cyclohex-3-enyl.
  • the term “cycloalkyl” refers to a single saturated all carbon ring having 3 to 8 carbon atoms.
  • Non-limiting examples of carbocycles include cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.
  • heteroaryl refers to a single aromatic ring that has at least one atom other than carbon in the ring, wherein the atom is selected from the group consisting of oxygen, nitrogen and sulfur; “heteroaryl” also includes multiple condensed ring systems that have at least one such aromatic ring, which multiple condensed ring systems are further described below.
  • heteroaryl includes single aromatic rings of from about 1 to 6 carbon atoms and about 1-4 heteroatoms selected from the group consisting of oxygen, nitrogen and sulfur. The sulfur and nitrogen atoms may also be present in an oxidized form provided the ring is aromatic.
  • heteroaryl ring systems include but are not limited to pyridyl, pyrimidinyl, oxazolyl or furyl.
  • “Heteroaryl” also includes multiple condensed ring systems (e.g., ring systems comprising 2, 3 or 4 rings) wherein a heteroaryl group, as defined above, is condensed with one or more rings selected from heteroaryls (to form for example a naphthyridinyl such as 1,8-naphthyridinyl), heterocycles, (to form for example a 1,2,3,4-tetrahydronaphthyridinyl such as 1,2,3,4-tetrahydro-1,8-naphthyridinyl), carbocycles (to form for example 5,6,7,8-tetrahydroquinolyl) and aryls (to form for example indazolyl) to form the multiple condensed ring system.
  • heteroaryl to form for example a naphthyrid
  • a heteroaryl (a single aromatic ring or multiple condensed ring system) has about 1-20 carbon atoms and about 1-6 heteroatoms within the heteroaryl ring.
  • a heteroaryl (a single aromatic ring or multiple condensed ring system) can also have about 5 to 20 or about 5 to 15 or about 5 to 10 members within the heteroaryl ring.
  • Such multiple condensed ring systems may be optionally substituted with one or more (e.g., 1, 2, 3 or 4) oxo groups on the carbocycle or heterocycle portions of the condensed ring.
  • the rings of the multiple condensed ring system can be connected to each other via fused, spiro and bridged bonds when allowed by valency requirements.
  • the individual rings of the multiple condensed ring system may be connected in any order relative to one another.
  • the point of attachment of a multiple condensed ring system (as defined above for a heteroaryl) can be at any position of the multiple condensed ring system including a heteroaryl, heterocycle, aryl or carbocycle portion of the multiple condensed ring system.
  • the point of attachment for a heteroaryl or heteroaryl multiple condensed ring system can be at any suitable atom of the heteroaryl or heteroaryl multiple condensed ring system including a carbon atom and a heteroatom (e.g., a nitrogen).
  • heteroaryls include but are not limited to pyridyl, pyrrolyl, pyrazinyl, pyrimidinyl, pyridazinyl, pyrazolyl, thienyl, indolyl, imidazolyl, oxazolyl, isoxazolyl, thiazolyl, furyl, oxadiazolyl, thiadiazolyl, quinolyl, isoquinolyl, benzothiazolyl, benzoxazolyl, indazolyl, quinoxalyl, quinazolyl, 5,6,7,8-tetrahydroisoquinolinyl benzofuranyl, benzimidazolyl, thianaphthenyl, pyrrolo[2,3-b]pyridinyl, quinazolinyl-4(3H)-one, triazolyl, 4,5,6,7-tetrahydro-1H-indazole and 3b,4,4
  • heteroaryl refers to a single aromatic ring containing at least one heteroatom.
  • the term includes 5-membered and 6-membered monocyclic aromatic rings that include one or more heteroatoms.
  • Non-limiting examples of heteroaryl include but are not limited to pyridyl, furyl, thiazole, pyrimidine, oxazole, and thiadiazole.
  • heterocyclyl or “heterocycle” as used herein refers to a single saturated or partially unsaturated ring that has at least one atom other than carbon in the ring, wherein the atom is selected from the group consisting of oxygen, nitrogen and sulfur; the term also includes multiple condensed ring systems that have at least one such saturated or partially unsaturated ring, which multiple condensed ring systems are further described below.
  • the term includes single saturated or partially unsaturated rings (e.g., 3, 4, 5, 6 or 7-membered rings) from about 1 to 6 carbon atoms and from about 1 to 3 heteroatoms selected from the group consisting of oxygen, nitrogen and sulfur in the ring.
  • the ring may be substituted with one or more (e.g., 1, 2 or 3) oxo groups and the sulfur and nitrogen atoms may also be present in their oxidized forms.
  • exemplary heterocycles include but are not limited to azetidinyl, tetrahydrofuranyl and piperidinyl.
  • heterocycle also includes multiple condensed ring systems (e.g., ring systems comprising 2, 3 or 4 rings) wherein a single heterocycle ring (as defined above) can be condensed with one or more groups selected from heterocycles (to form for example a 1,8-decahydronapthyridinyl), carbocycles (to form for example a decahydroquinolyl) and aryls to form the multiple condensed ring system.
  • a heterocycle a single saturated or single partially unsaturated ring or multiple condensed ring system
  • Such multiple condensed ring systems may be optionally substituted with one or more (e.g., 1, 2, 3 or 4) oxo groups on the carbocycle or heterocycle portions of the multiple condensed ring.
  • the rings of the multiple condensed ring system can be connected to each other via fused, spiro and bridged bonds when allowed by valency requirements. It is to be understood that the individual rings of the multiple condensed ring system may be connected in any order relative to one another.
  • a heterocycle (a single saturated or single partially unsaturated ring or multiple condensed ring system) has about 3-20 atoms including about 1-6 heteroatoms within the heterocycle ring system.
  • the point of attachment of a multiple condensed ring system can be at any position of the multiple condensed ring system including a heterocycle, aryl and carbocycle portion of the ring. It is also to be understood that the point of attachment for a heterocycle or heterocycle multiple condensed ring system can be at any suitable atom of the heterocycle or heterocycle multiple condensed ring system including a carbon atom and a heteroatom (e.g., a nitrogen).
  • the term heterocycle includes a C 2-20 heterocycle.
  • heterocycle includes a C 2-7 heterocycle.
  • the term heterocycle includes a C 2-5 heterocycle.
  • heterocycle includes a C 2-4 heterocycle.
  • exemplary heterocycles include, but are not limited to aziridinyl, azetidinyl, pyrrolidinyl, piperidinyl, homopiperidinyl, morpholinyl, thiomorpholinyl, piperazinyl, tetrahydrofuranyl, dihydrooxazolyl, tetrahydropyranyl, tetrahydrothiopyranyl, 1,2,3,4-tetrahydroquinolyl, benzoxazinyl, dihydrooxazolyl, chromanyl, 1,2-dihydropyridinyl, 2,3-dihydrobenzofuranyl, 1,3-benzodioxolyl, 1,4-benzodioxanyl, spiro[cyclopropane-1,1′-isoindolinyl]-3′-one, isoindolinyl-1-one, 2-oxa
  • heterocycle refers to a monocyclic, saturated or partially unsaturated, 3-8 membered ring having at least one heteroatom.
  • the term includes a monocyclic, saturated or partially unsaturated, 4, 5, 6, or 7 membered ring having at least one heteroatom.
  • Non-limiting examples of heterocycle include aziridine, azetidine, pyrrolidine, piperidine, piperidine, piperazine, oxirane, morpholine, and thiomorpholine.
  • the term “9- or 10-membered heterobicycle” as used herein refers to a partially unsaturated or aromatic fused bicyclic ring system having at least one heteroatom.
  • 9- or 10-membered heterobicycle includes a bicyclic ring system having a benzo ring fused to a 5-membered or 6-membered saturated, partially unsaturated, or aromatic ring that contains one or more heteroatoms.
  • heteroatom is meant to include oxygen (O), nitrogen (N), sulfur (S) and silicon (Si).
  • oxygen and sulfur can be in an oxidized form when feasible.
  • chiral refers to molecules which have the property of non-superimposability of the mirror image partner, while the term “achiral” refers to molecules which are superimposable on their mirror image partner.
  • stereoisomers refers to compounds which have identical chemical constitution, but differ with regard to the arrangement of the atoms or groups in space.
  • a wavy line “ ” that intersects a bond in a chemical structure indicates the point of attachment of the bond that the wavy bond intersects in the chemical structure to the remainder of a molecule.
  • Diastereomer refers to a stereoisomer with two or more centers of chirality and whose molecules are not mirror images of one another. Diastereomers have different physical properties, e.g. melting points, boiling points, spectral properties, and reactivities. Mixtures of diastereomers can separate under high resolution analytical procedures such as electrophoresis and chromatography.
  • Enantiomers refer to two stereoisomers of a compound which are non-superimposable mirror images of one another.
  • the compounds of the invention can contain asymmetric or chiral centers, and therefore exist in different stereoisomeric forms. It is intended that all stereoisomeric forms of the compounds of the invention, including but not limited to, diastereomers, enantiomers and atropisomers, as well as mixtures thereof such as racemic mixtures, form part of the present invention.
  • racemic mixture A 50:50 mixture of enantiomers is referred to as a racemic mixture or a racemate, which can occur where there has been no stereoselection or stereospecificity in a chemical reaction or process.
  • racemic mixture and “racemate” refer to an equimolar mixture of two enantiomeric species, devoid of optical activity.
  • the atom to which the bond is attached includes all stereochemical possibilities.
  • a bond in a compound formula herein is drawn in a defined stereochemical manner (e.g. bold, bold-wedge, dashed or dashed-wedge)
  • a bond in a compound formula herein is drawn in a defined stereochemical manner (e.g. bold, bold-wedge, dashed or dashed-wedge)
  • the atom to which the stereochemical bond is attached is enriched in the absolute stereoisomer depicted unless otherwise noted.
  • the compound may be at least 51% the absolute stereoisomer depicted.
  • the compound may be at least 80% the absolute stereoisomer depicted.
  • the compound may be at least 90% the absolute stereoisomer depicted.
  • the compound may be at least 95% the absolute stereoisomer depicted. In another embodiment, the compound may be at least 97% the absolute stereoisomer depicted. In another embodiment, the compound may be at least 98% the absolute stereoisomer depicted. In another embodiment, the compound may be at least 99% the absolute stereoisomer depicted.
  • tautomer or “tautomeric form” refers to structural isomers of different energies which are interconvertible via a low energy barrier.
  • proton tautomers also known as prototropic tautomers
  • Valence tautomers include interconversions by reorganization of some of the bonding electrons.
  • solvate refers to an association or complex of one or more solvent molecules and a compound of the invention.
  • solvents that form solvates include, but are not limited to, water, isopropanol, ethanol, methanol, DMSO, ethyl acetate, acetic acid, and ethanolamine.
  • hydrate refers to the complex where the solvent molecule is water.
  • protecting group refers to a substituent that is commonly employed to block or protect a particular functional group on a compound.
  • an “amino-protecting group” is a substituent attached to an amino group that blocks or protects the amino functionality in the compound.
  • Suitable amino-protecting groups include acetyl, trifluoroacetyl, t-butoxycarbonyl (BOC), benzyloxycarbonyl (CBZ) and 9-fluorenylmethylenoxycarbonyl (Fmoc).
  • a “hydroxy-protecting group” refers to a substituent of a hydroxy group that blocks or protects the hydroxy functionality. Suitable protecting groups include acetyl and silyl.
  • a “carboxy-protecting group” refers to a substituent of the carboxy group that blocks or protects the carboxy functionality.
  • Common carboxy-protecting groups include phenylsulfonylethyl, cyanoethyl, 2-(trimethylsilyl)ethyl, 2-(trimethylsilyl)ethoxymethyl, 2-(p-toluenesulfonyl)ethyl, 2-(p-nitrophenylsulfenyl)ethyl, 2-(diphenylphosphino)-ethyl, nitroethyl and the like.
  • protecting groups and their use see P. G. M. Wuts and T. W. Greene, Greene's Protective Groups in Organic Synthesis 4 th edition, Wiley-Interscience, New York, 2006.
  • mammal includes, but is not limited to, humans, mice, rats, guinea pigs, monkeys, dogs, cats, horses, cows, pigs, and sheep.
  • salts are meant to include salts of the active compounds which are prepared with relatively nontoxic acids or bases, depending on the particular substituents found on the compounds described herein.
  • base addition salts can be obtained by contacting the neutral form of such compounds with a sufficient amount of the desired base, either neat or in a suitable inert solvent.
  • salts derived from pharmaceutically-acceptable inorganic bases include aluminum, ammonium, calcium, copper, ferric, ferrous, lithium, magnesium, manganic, manganous, potassium, sodium, zinc and the like.
  • Salts derived from pharmaceutically-acceptable organic bases include salts of primary, secondary and tertiary amines, including substituted amines, cyclic amines, naturally-occurring amines and the like, such as arginine, betaine, caffeine, choline, N,N′-dibenzylethylenediamine, diethylamine, 2-diethylaminoethanol, 2-dimethylaminoethanol, ethanolamine, ethylenediamine, N-ethylmorpholine, N-ethylpiperidine, glucamine, glucosamine, histidine, hydrabamine, isopropylamine, lysine, methylglucamine, morpholine, piperazine, piperidine, polyamine resins, procaine, purines, theobromine, triethylamine, trimethylamine, tripropylamine, tromethamine and the like.
  • acid addition salts can be obtained by contacting the neutral form of such compounds with a sufficient amount of the desired acid, either neat or in a suitable inert solvent.
  • pharmaceutically acceptable acid addition salts include those derived from inorganic acids like hydrochloric, hydrobromic, nitric, carbonic, monohydrogencarbonic, phosphoric, monohydrogenphosphoric, dihydrogenphosphoric, sulfuric, monohydrogensulfuric, hydriodic, or phosphorous acids and the like, as well as the salts derived from relatively nontoxic organic acids like acetic, propionic, isobutyric, malonic, benzoic, succinic, suberic, fumaric, mandelic, phthalic, benzenesulfonic, p-tolylsulfonic, citric, tartaric, methanesulfonic, and the like.
  • salts of amino acids such as arginate and the like, and salts of organic acids like glucuronic or galactunoric acids and the like (see, for example, Berge, S. M., et al., “Pharmaceutical Salts”, Journal of Pharmaceutical Science, 1977, 66, 1-19).
  • Certain specific compounds of the present invention contain both basic and acidic functionalities that allow the compounds to be converted into either base or acid addition salts.
  • the neutral forms of the compounds can be regenerated by contacting the salt with a base or acid and isolating the parent compound in the conventional manner.
  • the parent form of the compound differs from the various salt forms in certain physical properties, such as solubility in polar solvents, but otherwise the salts are equivalent to the parent form of the compound for the purposes of the present invention.
  • W ⁇ is a pharmaceutically acceptable anion.
  • W ⁇ is chloride, bromide, iodide, tosylate, methanesulfonate, acetate, citrate, malonate, tartrate, succinate, fumarate, benzoate, ascorbate, ⁇ -ketoglutarate, ⁇ -glycerophosphate, benzenesulfonate, lactate, bicarbonate, malate, maleate, carbonate, methylbromide, chloride, nitrate, gluconate, salicylate, glutamate, stearate, or sulfate.
  • the present invention provides compounds which are in a prodrug form.
  • prodrug refers to those compounds that readily undergo chemical changes under physiological conditions to provide the compounds of the present invention.
  • prodrugs can be converted to the compounds of the present invention by chemical or biochemical methods in an ex vivo environment. For example, prodrugs can be slowly converted to the compounds of the present invention when placed in a transdermal patch reservoir with a suitable enzyme or chemical reagent.
  • Prodrugs of the invention include compounds wherein an amino acid residue, or a polypeptide chain of two or more (e.g., two, three or four) amino acid residues, is covalently joined through an amide or ester bond to a free amino, hydroxy or carboxylic acid group of a compound of the present invention.
  • the amino acid residues include but are not limited to the 20 naturally occurring amino acids commonly designated by three letter symbols and also includes phosphoserine, phosphothreonine, phosphotyrosine, 4-hydroxyproline, hydroxylysine, demosine, isodemosine, gamma-carboxyglutamate, hippuric acid, octahydroindole-2-carboxylic acid, statine, 1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid, penicillamine, ornithine, 3-methylhistidine, norvaline, beta-alanine, gamma-aminobutyric acid, citrulline, homocysteine, homoserine, methyl-alanine, para-benzoylphenylalanine, phenylglycine, propargylglycine, sarcosine, methionine sulfone and tert-butylglycine.
  • prodrugs are also encompassed.
  • a free carboxyl group of a compound of the invention can be derivatized as an amide or alkyl ester.
  • compounds of this invention comprising free hydroxy groups can be derivatized as prodrugs by converting the hydroxy group into a group such as, but not limited to, a phosphate ester, hemisuccinate, dimethylaminoacetate, or phosphoryloxymethyloxycarbonyl group, as outlined in Fleisher, D. et al., (1996) Improved oral drug delivery: solubility limitations overcome by the use of prodrugs Advanced Drug Delivery Reviews, 19:115.
  • Carbamate prodrugs of hydroxy and amino groups are also included, as are carbonate prodrugs, sulfonate esters and sulfate esters of hydroxy groups.
  • Derivatization of hydroxy groups as (acyloxy)methyl and (acyloxy)ethyl ethers, wherein the acyl group can be an alkyl ester optionally substituted with groups including, but not limited to, ether, amine and carboxylic acid functionalities, or where the acyl group is an amino acid ester as described above, are also encompassed.
  • Prodrugs of this type are described in J. Med. Chem., (1996), 39:10.
  • More specific examples include replacement of the hydrogen atom of the alcohol group with a group such as (C 1-6 )alkanoyloxymethyl, 1-((C 1-6 )alkanoyloxy)ethyl, 1-methyl-1-((C 1-6 )alkanoyloxy)ethyl, (C 1-6 )alkoxycarbonyloxymethyl, N—(C 1-6 )alkoxycarbonylaminomethyl, succinoyl, (C 1-6 )alkanoyl, alpha-amino(C 1-4 )alkanoyl, arylacyl and alpha-aminoacyl, or alpha-aminoacyl-alpha-aminoacyl, where each alpha-aminoacyl group is independently selected from the naturally occurring L-amino acids, P(O)(OH) 2 , —P(O)(O(C 1-6 )alkyl) 2 or glycosyl (the radical resulting from the removal of a hydroxyl group of
  • prodrug derivatives see, for example, a) Design of Prodrugs, edited by H. Bundgaard, (Elsevier, 1985) and Methods in Enzymology, Vol. 42, p. 309-396, edited by K. Widder, et al. (Academic Press, 1985); b) A Textbook of Drug Design and Development, edited by Krogsgaard-Larsen and H. Bundgaard, Chapter 5 “Design and Application of Prodrugs,” by H. Bundgaard p. 113-191 (1991); c) H. Bundgaard, Advanced Drug Delivery Reviews, 8:1-38 (1992); d) H.
  • a “metabolite” refers to a product produced through metabolism in the body of a specified compound or salt thereof. Such products can result for example from the oxidation, reduction, hydrolysis, amidation, deamidation, esterification, deesterification, enzymatic cleavage, and the like, of the administered compound.
  • Metabolite products typically are identified by preparing a radiolabelled (e.g., 14 C or 3 H) isotope of a compound of the invention, administering it parenterally in a detectable dose (e.g., greater than about 0.5 mg/kg) to an animal such as rat, mouse, guinea pig, monkey, or to man, allowing sufficient time for metabolism to occur (typically about 30 seconds to 30 hours) and isolating its conversion products from the urine, blood or other biological samples.
  • a detectable dose e.g., greater than about 0.5 mg/kg
  • metabolites In general, analysis of metabolites is done in the same way as conventional drug metabolism studies well known to those skilled in the art.
  • the metabolite products so long as they are not otherwise found in vivo, are useful in diagnostic assays for therapeutic dosing of the compounds of the invention.
  • the present invention provides for compounds of Formula I and its embodiments as described hereinbove.
  • the compound is selected from compounds of formula I as described in the Examples herein and salts thereof.
  • compositions and medicaments comprising a compound of formula I or and embodiment thereof and at least one pharmaceutically acceptable carrier, diluent or excipient.
  • the compositions of the invention can be used to selectively inhibit NaV1.7 in patients (e.g, humans).
  • composition is intended to encompass a product comprising the specified ingredients in the specified amounts, as well as any product which results, directly or indirectly, from combination of the specified ingredients in the specified amounts.
  • pharmaceutically acceptable it is meant the carrier, diluent or excipient must be compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.
  • the invention provides for pharmaceutical compositions (or medicaments) comprising a compound of formula I or an embodiment thereof, and its stereoisomers, geometric isomers, tautomers, solvates, metabolites, isotopes, pharmaceutically acceptable salts, or prodrugs thereof) and a pharmaceutically acceptable carrier, diluent or excipient.
  • the invention provides for preparing compositions (or medicaments) comprising compounds of the invention.
  • the invention provides for administering compounds of formula I or its embodiments and compositions comprising compounds of formula I or an embodiment thereof to a patient (e.g., a human patient) in need thereof.
  • compositions are formulated, dosed, and administered in a fashion consistent with good medical practice.
  • Factors for consideration in this context include the particular disorder being treated, the particular mammal being treated, the clinical condition of the individual patient, the cause of the disorder, the site of delivery of the agent, the method of administration, the scheduling of administration, and other factors known to medical practitioners.
  • the effective amount of the compound to be administered will be governed by such considerations, and is the minimum amount necessary to inhibit NaV1.7 activity as required to prevent or treat the undesired disease or disorder, such as for example, pain. For example, such amount may be below the amount that is toxic to normal cells, or the mammal as a whole.
  • the therapeutically effective amount of the compound of the invention administered parenterally per dose will be in the range of about 0.01-100 mg/kg, alternatively about e.g., 0.1 to 20 mg/kg of patient body weight per day, with the typical initial range of compound used being 0.3 to 15 mg/kg/day.
  • the daily does is, in certain embodiments, given as a single daily dose or in divided doses two to six times a day, or in sustained release form. In the case of a 70 kg adult human, the total daily dose will generally be from about 7 mg to about 1,400 mg. This dosage regimen may be adjusted to provide the optimal therapeutic response.
  • the compounds may be administered on a regimen of 1 to 4 times per day, preferably once or twice per day.
  • the compounds of the present invention may be administered in any convenient administrative form, e.g., tablets, powders, capsules, solutions, dispersions, suspensions, syrups, sprays, suppositories, gels, emulsions, patches, etc.
  • Such compositions may contain components conventional in pharmaceutical preparations, e.g., diluents, carriers, pH modifiers, sweeteners, bulking agents, and further active agents.
  • the compounds of the invention may be administered by any suitable means, including oral, topical (including buccal and sublingual), rectal, vaginal, transdermal, parenteral, subcutaneous, intraperitoneal, intrapulmonary, intradermal, intrathecal and epidural and intranasal, and, if desired for local treatment, intralesional administration.
  • Parenteral infusions include intramuscular, intravenous, intraarterial, intraperitoneal, intracerebral, intraocular, intralesional or subcutaneous administration.
  • compositions comprising compounds of formula I or an embodiment thereof are normally formulated in accordance with standard pharmaceutical practice as a pharmaceutical composition.
  • a typical formulation is prepared by mixing a compound of the present invention and a diluent, carrier or excipient. Suitable diluents, carriers and excipients are well known to those skilled in the art and are described in detail in, e.g., Ansel, Howard C., et al., Ansel's Pharmaceutical Dosage Forms and Drug Delivery Systems. Philadelphia: Lippincott, Williams & Wilkins, 2004; Gennaro, Alfonso R., et al. Remington: The Science and Practice of Pharmacy. Philadelphia: Lippincott, Williams & Wilkins, 2000; and Rowe, Raymond C.
  • the formulations may also include one or more buffers, stabilizing agents, surfactants, wetting agents, lubricating agents, emulsifiers, suspending agents, preservatives, antioxidants, opaquing agents, glidants, processing aids, colorants, sweeteners, perfuming agents, flavoring agents, diluents and other known additives to provide an elegant presentation of the drug (i.e., a compound of the present invention or pharmaceutical composition thereof) or aid in the manufacturing of the pharmaceutical product (i.e., medicament).
  • buffers stabilizing agents, surfactants, wetting agents, lubricating agents, emulsifiers, suspending agents, preservatives, antioxidants, opaquing agents, glidants, processing aids, colorants, sweeteners, perfuming agents, flavoring agents, diluents and other known additives to provide an elegant presentation of the drug (i.e., a compound of the present invention or pharmaceutical composition thereof) or aid in the manufacturing
  • Suitable carriers, diluents and excipients are well known to those skilled in the art and include materials such as carbohydrates, waxes, water soluble and/or swellable polymers, hydrophilic or hydrophobic materials, gelatin, oils, solvents, water and the like.
  • the particular carrier, diluent or excipient used will depend upon the means and purpose for which a compound of the present invention is being applied.
  • Solvents are generally selected based on solvents recognized by persons skilled in the art as safe (GRAS) to be administered to a mammal.
  • safe solvents are non-toxic aqueous solvents such as water and other non-toxic solvents that are soluble or miscible in water.
  • Suitable aqueous solvents include water, ethanol, propylene glycol, polyethylene glycols (e.g., PEG 400, PEG 300), etc. and mixtures thereof.
  • the formulations can also include one or more buffers, stabilizing agents, surfactants, wetting agents, lubricating agents, emulsifiers, suspending agents, preservatives, antioxidants, opaquing agents, glidants, processing aids, colorants, sweeteners, perfuming agents, flavoring agents and other known additives to provide an elegant presentation of the drug (i.e., a compound of the present invention or pharmaceutical composition thereof) or aid in the manufacturing of the pharmaceutical product (i.e., medicament).
  • Acceptable diluents, carriers, excipients and stabilizers are nontoxic to recipients at the dosages and concentrations employed, and include buffers such as phosphate, citrate and other organic acids; antioxidants including ascorbic acid and methionine; preservatives (such as octadecyldimethylbenzyl ammonium chloride; hexamethonium chloride; benzalkonium chloride, benzethonium chloride; phenol, butyl or benzyl alcohol; alkyl parabens such as methyl or propyl paraben; catechol; resorcinol; cyclohexanol; 3-pentanol; and m-cresol); low molecular weight (less than about 10 residues) polypeptides; proteins, such as serum albumin, gelatin, or immunoglobulins; hydrophilic polymers such as polyvinylpyrrolidone; amino acids such as glycine, glutamine, asparagine,
  • a active pharmaceutical ingredient of the invention can also be entrapped in microcapsules prepared, for example, by coacervation techniques or by interfacial polymerization, for example, hydroxymethylcellulose or gelatin-microcapsules and poly-(methylmethacylate) microcapsules, respectively, in colloidal drug delivery systems (for example, liposomes, albumin microspheres, microemulsions, nano-particles and nanocapsules) or in macroemulsions.
  • colloidal drug delivery systems for example, liposomes, albumin microspheres, microemulsions, nano-particles and nanocapsules
  • macroemulsions for example, liposomes, albumin microspheres, microemulsions, nano-particles and nanocapsules
  • Sustained-release preparations of a compound of the invention can be prepared.
  • suitable examples of sustained-release preparations include semipermeable matrices of solid hydrophobic polymers containing a compound of formula I or an embodiment thereof, which matrices are in the form of shaped articles, e.g., films, or microcapsules.
  • sustained-release matrices include polyesters, hydrogels (for example, poly(2-hydroxyethyl-methacrylate), or poly(vinyl alcohol)), polylactides (U.S. Pat. No.
  • Sustained release compositions also include liposomally entrapped compounds, which can be prepared by methods known per se (Epstein et al., Proc. Natl. Acad. Sci. U.S.A. 82:3688, 1985; Hwang et al., Proc. Natl. Acad. Sci. U.S.A. 77:4030, 1980; U.S. Pat. Nos. 4,485,045 and 4,544,545; and EP 102,324A).
  • the liposomes are of the small (about 200-800 Angstroms) unilamelar type in which the lipid content is greater than about 30 mol % cholesterol, the selected proportion being adjusted for the optimal therapy.
  • the formulations include those suitable for the administration routes detailed herein.
  • the formulations can conveniently be presented in unit dosage form and can be prepared by any of the methods well known in the art of pharmacy. Techniques and formulations generally are found in Remington: The Science and Practice of Pharmacy: Remington the Science and Practice of Pharmacy (2005) 21 st Edition, Lippincott Williams & Wilkins, Philidelphia, Pa. Such methods include the step of bringing into association the active ingredient with the carrier which constitutes one or more accessory ingredients.
  • the formulations are prepared by uniformly and intimately bringing into association the active ingredient with liquid carriers, diluents or excipients or finely divided solid carriers, diluents or excipients, or both, and then, if necessary, shaping the product.
  • a typical formulation is prepared by mixing a compound of the present invention and a carrier, diluent or excipient.
  • the formulations can be prepared using conventional dissolution and mixing procedures.
  • the bulk drug substance i.e., compound of the present invention or stabilized form of the compound (e.g., complex with a cyclodextrin derivative or other known complexation agent) is dissolved in a suitable solvent in the presence of one or more of the excipients described above.
  • a compound of the present invention is typically formulated into pharmaceutical dosage forms to provide an easily controllable dosage of the drug and to enable patient compliance with the prescribed regimen.
  • compounds of formula I or an embodiment thereof may be formulated by mixing at ambient temperature at the appropriate pH, and at the desired degree of purity, with physiologically acceptable carriers, i.e., carriers that are non-toxic to recipients at the dosages and concentrations employed into a galenical administration form.
  • physiologically acceptable carriers i.e., carriers that are non-toxic to recipients at the dosages and concentrations employed into a galenical administration form.
  • the pH of the formulation depends mainly on the particular use and the concentration of compound, but preferably ranges anywhere from about 3 to about 8.
  • a compound of formula I (or an embodiment thereof) is formulated in an acetate buffer, at pH 5.
  • the compounds of formula I or an embodiment thereof are sterile.
  • the compound may be stored, for example, as a solid or amorphous composition, as a lyophilized formulation or as an aqueous solution.
  • Formulations of a compound of the invention e.g., compound of formula I or an embodiment thereof
  • suitable for oral administration can be prepared as discrete units such as pills, capsules, cachets or tablets each containing a predetermined amount of a compound of the invention.
  • Compressed tablets can be prepared by compressing in a suitable machine the active ingredient in a free-flowing form such as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, preservative, surface active or dispersing agent. Molded tablets can be made by molding in a suitable machine a mixture of the powdered active ingredient moistened with an inert liquid diluent. The tablets can optionally be coated or scored and optionally are formulated so as to provide slow or controlled release of the active ingredient therefrom.
  • Tablets, troches, lozenges, aqueous or oil suspensions, dispersible powders or granules, emulsions, hard or soft capsules, e.g., gelatin capsules, syrups or elixirs can be prepared for oral use.
  • Formulations of a compound of the invention (e.g., compound of formula I or an embodiment thereof) intended for oral use can be prepared according to any method known to the art for the manufacture of pharmaceutical compositions and such compositions can contain one or more agents including sweetening agents, flavoring agents, coloring agents and preserving agents, in order to provide a palatable preparation. Tablets containing the active ingredient in admixture with non-toxic pharmaceutically acceptable excipient which are suitable for manufacture of tablets are acceptable.
  • excipients can be, for example, inert diluents, such as calcium or sodium carbonate, lactose, calcium or sodium phosphate; granulating and disintegrating agents, such as maize starch, or alginic acid; binding agents, such as starch, gelatin or acacia; and lubricating agents, such as magnesium stearate, stearic acid or talc. Tablets can be uncoated or can be coated by known techniques including microencapsulation to delay disintegration and adsorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl monostearate or glyceryl distearate alone or with a wax can be employed.
  • inert diluents such as calcium or sodium carbonate, lactose, calcium or sodium phosphate
  • granulating and disintegrating agents such as maize starch, or alginic acid
  • binding agents such as starch, ge
  • An example of a suitable oral administration form is a tablet containing about 1 mg, 5 mg, 10 mg, 25 mg, 30 mg, 50 mg, 80 mg, 100 mg, 150 mg, 250 mg, 300 mg and 500 mg of the compound of the invention compounded with about 90-30 mg anhydrous lactose, about 5-40 mg sodium croscarmellose, about 5-30 mg polyvinylpyrrolidone (PVP) K30, and about 1-10 mg magnesium stearate.
  • the powdered ingredients are first mixed together and then mixed with a solution of the PVP.
  • the resulting composition can be dried, granulated, mixed with the magnesium stearate and compressed to tablet form using conventional equipment.
  • An example of an aerosol formulation can be prepared by dissolving the compound, for example 5-400 mg, of the invention in a suitable buffer solution, e.g. a phosphate buffer, adding a tonicifier, e.g. a salt such sodium chloride, if desired.
  • a suitable buffer solution e.g. a phosphate buffer
  • a tonicifier e.g. a salt such sodium chloride
  • the solution may be filtered, e.g., using a 0.2 micron filter, to remove impurities and contaminants.
  • the formulations are preferably applied as a topical ointment or cream containing the active ingredient(s) in an amount of, for example, 0.075 to 20% w/w.
  • the active ingredient can be employed with either a paraffinic or a water-miscible ointment base.
  • the active ingredients can be formulated in a cream with an oil-in-water cream base.
  • the aqueous phase of the cream base can include a polyhydric alcohol, i.e., an alcohol having two or more hydroxyl groups such as propylene glycol, butane 1,3-diol, mannitol, sorbitol, glycerol and polyethylene glycol (including PEG 400) and mixtures thereof.
  • the topical formulations can desirably include a compound which enhances absorption or penetration of the active ingredient through the skin or other affected areas. Examples of such dermal penetration enhancers include dimethyl sulfoxide and related analogs.
  • the oily phase of the emulsions of this invention can be constituted from known ingredients in a known manner. While the phase can comprise merely an emulsifier, it desirably comprises a mixture of at least one emulsifier with a fat or an oil or with both a fat and an oil. Preferably, a hydrophilic emulsifier is included together with a lipophilic emulsifier which acts as a stabilizer. It is also preferred to include both an oil and a fat.
  • Emulsifiers and emulsion stabilizers suitable for use in the formulation of the invention include Tween® 60, Span® 80, cetostearyl alcohol, benzyl alcohol, myristyl alcohol, glyceryl mono-stearate and sodium lauryl sulfate.
  • a pharmaceutical composition according to the invention it is desired to administer an effective amount of a pharmaceutical composition according to the invention to target area, e.g., skin surfaces, mucous membranes, and the like, which are adjacent to peripheral neurons which are to be treated.
  • This amount will generally range from about 0.0001 mg to about 1 g of a compound of the invention per application, depending upon the area to be treated, whether the use is diagnostic, prophylactic or therapeutic, the severity of the symptoms, and the nature of the topical vehicle employed.
  • a preferred topical preparation is an ointment, wherein about 0.001 to about 50 mg of active ingredient is used per cc of ointment base.
  • the pharmaceutical composition can be formulated as transdermal compositions or transdermal delivery devices (“patches”). Such compositions include, for example, a backing, active compound reservoir, a control membrane, liner and contact adhesive. Such transdermal patches may be used to provide continuous pulsatile, or on demand delivery of the compounds of the present invention as desired.
  • Aqueous suspensions of a compound of the invention contain the active materials in admixture with excipients suitable for the manufacture of aqueous suspensions.
  • excipients include a suspending agent, such as sodium carboxymethylcellulose, croscarmellose, povidone, methylcellulose, hydroxypropyl methylcellulose, sodium alginate, polyvinylpyrrolidone, gum tragacanth and gum acacia, and dispersing or wetting agents such as a naturally occurring phosphatide (e.g., lecithin), a condensation product of an alkylene oxide with a fatty acid (e.g., polyoxyethylene stearate), a condensation product of ethylene oxide with a long chain aliphatic alcohol (e.g., heptadecaethyleneoxycetanol), a condensation product of ethylene oxide with a partial ester derived from a fatty acid and a hexitol anhydride (
  • the aqueous suspension can also contain one or more preservatives such as ethyl or n-propyl p-hydroxybenzoate, one or more coloring agents, one or more flavoring agents and one or more sweetening agents, such as sucrose or saccharin.
  • preservatives such as ethyl or n-propyl p-hydroxybenzoate
  • coloring agents such as a coloring agent
  • flavoring agents such as sucrose or saccharin.
  • sweetening agents such as sucrose or saccharin.
  • Formulations of a compound of the invention can be in the form of a sterile injectable preparation, such as a sterile injectable aqueous or oleaginous suspension.
  • a sterile injectable preparation such as a sterile injectable aqueous or oleaginous suspension.
  • This suspension can be formulated according to the known art using those suitable dispersing or wetting agents and suspending agents which have been mentioned above.
  • the sterile injectable preparation can also be a sterile injectable solution or suspension in a non-toxic parenterally acceptable diluent or solvent, such as a solution in 1,3-butanediol or prepared as a lyophilized powder.
  • Suitable vehicles and solvents that can be employed are water, Ringer's solution and isotonic sodium chloride solution.
  • sterile fixed oils can conventionally be employed as a solvent or suspending medium.
  • any bland fixed oil can be employed including synthetic mono- or diglycerides.
  • fatty acids such as oleic acid can likewise be used in the preparation of injectables.
  • a time-release formulation intended for oral administration to humans can contain approximately 1 to 1000 mg of active material compounded with an appropriate and convenient amount of carrier material which can vary from about 5 to about 95% of the total compositions (weight:weight).
  • the pharmaceutical composition can be prepared to provide easily measurable amounts for administration.
  • an aqueous solution intended for intravenous infusion can contain from about 3 to 500 ⁇ g of the active ingredient per milliliter of solution in order that infusion of a suitable volume at a rate of about 30 mL/hr can occur.
  • Formulations suitable for parenteral administration include aqueous and non-aqueous sterile injection solutions which can contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which can include suspending agents and thickening agents.
  • Formulations suitable for topical administration to the eye also include eye drops wherein the active ingredient is dissolved or suspended in a suitable carrier, especially an aqueous solvent for the active ingredient.
  • the active ingredient is preferably present in such formulations in a concentration of about 0.5 to 20% w/w, for example about 0.5 to 10% w/w, for example about 1.5% w/w.
  • Formulations suitable for topical administration in the mouth include lozenges comprising the active ingredient in a flavored basis, usually sucrose and acacia or tragacanth; pastilles comprising the active ingredient in an inert basis such as gelatin and glycerin, or sucrose and acacia; and mouthwashes comprising the active ingredient in a suitable liquid carrier.
  • Formulations for rectal administration can be presented as a suppository with a suitable base comprising for example cocoa butter or a salicylate.
  • Formulations suitable for intrapulmonary or nasal administration have a particle size for example in the range of 0.1 to 500 microns (including particle sizes in a range between 0.1 and 500 microns in increments microns such as 0.5, 1, 30 microns, 35 microns, etc.), which is administered by rapid inhalation through the nasal passage or by inhalation through the mouth so as to reach the alveolar sacs.
  • Suitable formulations include aqueous or oily solutions of the active ingredient.
  • Formulations suitable for aerosol or dry powder administration can be prepared according to conventional methods and can be delivered with other therapeutic agents such as compounds heretofore used in the treatment of disorders as described below.
  • the formulations can be packaged in unit-dose or multi-dose containers, for example sealed ampoules and vials, and can be stored in a freeze-dried (lyophilized) condition requiring only the addition of the sterile liquid carrier, for example water, for injection immediately prior to use.
  • sterile liquid carrier for example water
  • Extemporaneous injection solutions and suspensions are prepared from sterile powders, granules and tablets of the kind previously described.
  • Preferred unit dosage formulations are those containing a daily dose or unit daily sub-dose, as herein above recited, or an appropriate fraction thereof, of the active ingredient.
  • certain embodiments of the invention provide for a compound of formula I (or an embodiment thereof) to traverse the blood-brain barrier.
  • Certain neurodegenerative diseases are associated with an increase in permeability of the blood-brain barrier, such that a compound of formula I (or an embodiment thereof) can be readily introduced to the brain.
  • the blood-brain barrier remains intact, several art-known approaches exist for transporting molecules across it, including, but not limited to, physical methods, lipid-based methods, and receptor and channel-based methods.
  • Physical methods of transporting a compound of formula I (or an embodiment thereof) across the blood-brain barrier include, but are not limited to, circumventing the blood-brain barrier entirely, or by creating openings in the blood-brain barrier.
  • Circumvention methods include, but are not limited to, direct injection into the brain (see, e.g., Papanastassiou et al., Gene Therapy 9:398-406, 2002), interstitial infusion/convection-enhanced delivery (see, e.g., Bobo et al., Proc. Natl. Acad. Sci. U.S.A. 91:2076-2080, 1994), and implanting a delivery device in the brain (see, e.g., Gill et al., Nature Med. 9:589-595, 2003; and Gliadel WafersTM, Guildford Pharmaceutical).
  • Methods of creating openings in the barrier include, but are not limited to, ultrasound (see, e.g., U.S.
  • Patent Publication No. 2002/0038086 osmotic pressure (e.g., by administration of hypertonic mannitol (Neuwelt, E. A., Implication of the Blood-Brain Barrier and its Manipulation, Volumes 1 and 2, Plenum Press, N.Y., 1989)), and permeabilization by, e.g., bradykinin or permeabilizer A-7 (see, e.g., U.S. Pat. Nos. 5,112,596, 5,268,164, 5,506,206, and 5,686,416).
  • Lipid-based methods of transporting a compound of formula I (or an embodiment thereof) across the blood-brain barrier include, but are not limited to, encapsulating the a compound of formula I (or an embodiment thereof) in liposomes that are coupled to antibody binding fragments that bind to receptors on the vascular endothelium of the blood-brain barrier (see, e.g., U.S. Patent Application Publication No. 2002/0025313), and coating a compound of formula I (or an embodiment thereof) in low-density lipoprotein particles (see, e.g., U.S. Patent Application Publication No. 2004/0204354) or apolipoprotein E (see, e.g., U.S. Patent Application Publication No. 2004/0131692).
  • Receptor and channel-based methods of transporting a compound of formula I (or an embodiment thereof) across the blood-brain barrier include, but are not limited to, using glucocorticoid blockers to increase permeability of the blood-brain barrier (see, e.g., U.S. Patent Application Publication Nos. 2002/0065259, 2003/0162695, and 2005/0124533); activating potassium channels (see, e.g., U.S. Patent Application Publication No. 2005/0089473), inhibiting ABC drug transporters (see, e.g., U.S. Patent Application Publication No.
  • the compounds can be administered continuously by infusion into the fluid reservoirs of the CNS, although bolus injection may be acceptable.
  • the inhibitors can be administered into the ventricles of the brain or otherwise introduced into the CNS or spinal fluid. Administration can be performed by use of an indwelling catheter and a continuous administration means such as a pump, or it can be administered by implantation, e.g., intracerebral implantation of a sustained-release vehicle. More specifically, the inhibitors can be injected through chronically implanted cannulas or chronically infused with the help of osmotic minipumps. Subcutaneous pumps are available that deliver proteins through a small tubing to the cerebral ventricles.
  • Highly sophisticated pumps can be refilled through the skin and their delivery rate can be set without surgical intervention.
  • suitable administration protocols and delivery systems involving a subcutaneous pump device or continuous intracerebroventricular infusion through a totally implanted drug delivery system are those used for the administration of dopamine, dopamine agonists, and cholinergic agonists to Alzheimer's disease patients and animal models for Parkinson's disease, as described by Harbaugh, J. Neural Transm. Suppl. 24:271, 1987; and DeYebenes et al., Mov. Disord. 2: 143, 1987.
  • a compound of formula I (or an embodiment thereof) used in the invention are formulated, dosed, and administered in a fashion consistent with good medical practice.
  • Factors for consideration in this context include the particular disorder being treated, the particular mammal being treated, the clinical condition of the individual patient, the cause of the disorder, the site of delivery of the agent, the method of administration, the scheduling of administration, and other factors known to medical practitioners.
  • a compound of formula I (or an embodiment thereof) need not be, but is optionally formulated with one or more agent currently used to prevent or treat the disorder in question. The effective amount of such other agents depends on the amount of a compound of the invention present in the formulation, the type of disorder or treatment, and other factors discussed above.
  • the appropriate dosage of a compound of formula I (or an embodiment thereof) (when used alone or in combination with other agents) will depend on the type of disease to be treated, the properties of the compound, the severity and course of the disease, whether the compound is administered for preventive or therapeutic purposes, previous therapy, the patient's clinical history and response to the compound, and the discretion of the attending physician.
  • the compound is suitably administered to the patient at one time or over a series of treatments.
  • about 1 ⁇ g/kg to 15 mg/kg (e.g., 0.1 mg/kg-10 mg/kg) of compound can be an initial candidate dosage for administration to the patient, whether, for example, by one or more separate administrations, or by continuous infusion.
  • One typical daily dosage might range from about 1 ⁇ g kg to 100 mg/kg or more, depending on the factors mentioned above. For repeated administrations over several days or longer, depending on the condition, the treatment would generally be sustained until a desired suppression of disease symptoms occurs.
  • One exemplary dosage of a compound of formula I (or an embodiment thereof) would be in the range from about 0.05 mg/kg to about 10 mg/kg. Thus, one or more doses of about 0.5 mg/kg, 2.0 mg/kg, 4.0 mg/kg, or 10 mg/kg (or any combination thereof) may be administered to the patient.
  • Such doses may be administered intermittently, e.g., every week or every three weeks (e.g., such that the patient receives from about two to about twenty, or, e.g., about six doses of the antibody).
  • An initial higher loading dose, followed by one or more lower doses may be administered.
  • An exemplary dosing regimen comprises administering an initial loading dose of about 4 mg/kg, followed by a weekly maintenance dose of about 2 mg kg of the compound.
  • other dosage regimens may be useful. The progress of this therapy is easily monitored by conventional techniques and assays.
  • typical daily dosages might range from, for example, about 1 g/kg to up to 100 mg/kg or more (e.g., about 1 ⁇ g kg to 1 mg/kg, about 1 ⁇ g/kg to about 5 mg/kg, about 1 mg kg to 10 mg/kg, about 5 mg/kg to about 200 mg/kg, about 50 mg/kg to about 150 mg/mg, about 100 mg/kg to about 500 mg/kg, about 100 mg/kg to about 400 mg/kg, and about 200 mg/kg to about 400 mg/kg), depending on the factors mentioned above.
  • the clinician will administer a compound until a dosage is reached that results in improvement in or, optimally, elimination of, one or more symptoms of the treated disease or condition. The progress of this therapy is easily monitored by conventional assays.
  • One or more agent provided herein may be administered together or at different times (e.g., one agent is administered prior to the administration of a second agent).
  • One or more agent may be administered to a subject using different techniques (e.g., one agent may be administered orally, while a second agent is administered via intramuscular injection or intranasally).
  • One or more agent may be administered such that the one or more agent has a pharmacologic effect in a subject at the same time.
  • one or more agent may be administered, such that the pharmacological activity of the first administered agent is expired prior the administration of one or more secondarily administered agents (e.g., 1, 2, 3, or 4 secondarily administered agents).
  • the compounds of the invention modulate, preferably inhibit, ion flux through a voltage-dependent sodium channel in a mammal, (e.g, a human). Any such modulation, whether it be partial or complete inhibition or prevention of ion flux, is sometimes referred to herein as “blocking” and corresponding compounds as “blockers” or “inhibitors”.
  • the compounds of the invention modulate the activity of a sodium channel downwards by inhibiting the voltage-dependent activity of the sodium channel, and/or reduce or prevent sodium ion flux across a cell membrane by preventing sodium channel activity such as ion flux.
  • the compounds of the invention are sodium channel blockers and are therefore useful for treating diseases and conditions in mammals, for example humans, and other organisms, including all those diseases and conditions which are the result of aberrant voltage-dependent sodium channel biological activity or which may be ameliorated by modulation of voltage-dependent sodium channel biological activity.
  • the compounds of the invention i.e., the compounds of formula (I) and embodiments and (or stereoisomers, geometric isomers, tautomers, solvates, metabolites, isotopes, pharmaceutically acceptable salts, or prodrugs thereof), are useful for treating diseases and conditions in mammals, for example humans, which are the result of aberrant voltage-dependent NaV1.7 biological activity or which may be ameliorated by the modulation, preferably the inhibition, of NaV1.7 biological activity.
  • the compounds of the invention selectively inhibit NaV1.7 over NaV1.5.
  • a sodium channel-mediated disease or condition refers to a disease or condition in a mammal, preferably a human, which is ameliorated upon modulation of the sodium channel and includes, but is not limited to, pain, central nervous conditions such as epilepsy, anxiety, depression and bipolar disease; cardiovascular conditions such as arrhythmias, atrial fibrillation and ventricular fibrillation; neuromuscular conditions such as restless leg syndrome and muscle paralysis or tetanus; neuroprotection against stroke, neural trauma and multiple sclerosis; and channelopathies such as erythromyalgia and familial rectal pain syndrome.
  • pain central nervous conditions
  • cardiovascular conditions such as arrhythmias, atrial fibrillation and ventricular fibrillation
  • neuromuscular conditions such as restless leg syndrome and muscle paralysis or tetanus
  • neuroprotection against stroke neural trauma and multiple sclerosis
  • channelopathies such as erythromyalgia and familial rectal pain syndrome.
  • the present invention relates to compounds, pharmaceutical compositions and methods of using the compounds and pharmaceutical compositions for the treatment of sodium channel-mediated diseases in mammals, preferably humans and preferably diseases and conditions related to pain, central nervous conditions such as epilepsy, anxiety, depression and bipolar disease; cardiovascular conditions such as arrhythmias, atrial fibrillation and ventricular fibrillation; neuromuscular conditions such as restless leg syndrome and muscle paralysis or tetanus; neuroprotection against stroke, neural trauma and multiple sclerosis; and channelopathies such as erythromyalgia and familial rectal pain syndrome, by administering to a mammal, for example a human, in need of such treatment an effective amount of a sodium channel blocker modulating, especially inhibiting, agent.
  • central nervous conditions such as epilepsy, anxiety, depression and bipolar disease
  • cardiovascular conditions such as arrhythmias, atrial fibrillation and ventricular fibrillation
  • neuromuscular conditions such as restless leg syndrome and muscle paralysis or tetanus
  • a sodium channel-mediated disease or condition also includes pain associated with HIV, HIV treatment induced neuropathy, trigeminal neuralgia, glossopharyngeal neuralgia, neuropathy secondary to metastatic infiltration, adiposis dolorosa, thalamic lesions, hypertension, autoimmune disease, asthma, drug addiction (e.g., opiate, benzodiazepine, amphetamine, cocaine, alcohol, butane inhalation), Alzheimer, dementia, age-related memory impairment, Korsakoff syndrome, restenosis, urinary dysfunction, incontinence, Parkinson's disease, cerebrovascular ischemia, neurosis, gastrointestinal disease, sickle cell anemia, transplant rejection, heart failure, myocardial infarction, reperfusion injury, intermittant claudication, angina, convulsion, respiratory disorders, cerebral or myocardial ischemias, long-QT syndrome, Catecholeminergic polymorphic ventricular tachycardia, ophthalmic diseases, spasticity, spastic
  • pain refers to all categories of pain and is recognized to include, but is not limited to, neuropathic pain, inflammatory pain, nociceptive pain, idiopathic pain, neuralgic pain, orofacial pain, burn pain, burning mouth syndrome, somatic pain, visceral pain, myofacial pain, dental pain, cancer pain, chemotherapy pain, trauma pain, surgical pain, post-surgical pain, childbirth pain, labor pain, chronic regional pain syndrome (CRPS), reflex sympathetic dystrophy, brachial plexus avulsion, neurogenic bladder, acute pain (e.g., musculoskeletal and post-operative pain), chronic pain, persistent pain, peripherally mediated pain, centrally mediated pain, chronic headache, migraine headache, familial hemiplegic migraine, conditions associated with cephalic pain, sinus headache, tension headache, phantom limb pain, peripheral nerve injury, pain following stroke, thalamic lesions, radiculopathy, HIV pain, post-herpetic pain, non-cardiac chest pain,
  • the present invention therefore also relates to compounds, pharmaceutical compositions and methods of using the compounds and pharmaceutical compositions for the treatment of diseases or conditions such as cancer and pruritus (itch).
  • Pruritus commonly known as itch
  • itch is a common dermatological condition. While the exact causes of pruritus are complex and incompletely understood, there has long been evidence that itch involves sensory neurons, especially C fibers, similar to those that mediate pain (Schmelz, M., et al., J. Neurosci. (1997), 17: 8003-8). In particular, it is believed that sodium influx through voltage-gated sodium channels is essential for the propagation of itch sensation from the skin. Transmission of the itch impulses results in the unpleasant sensation that elicits the desire or reflex to scratch.
  • pruritus can be elicited by histamine or PAR-2 agonists such as mucunain that activate distinct populations of C fibers (Namer, B., et al., J. Neurophysiol. (2008), 100: 2062-9).
  • PAR-2 agonists such as mucunain that activate distinct populations of C fibers
  • a variety of neurotrophic peptides are known to mediate itch in animal models (Wang, H., and Yosipovitch, G., International Journal of Dermatology (2010), 49: 1-11). Itch can also be elicited by opioids, evidence of distinct pharmacology from that of pain responses.
  • the compounds of the invention can also be useful for treating pruritus.
  • the rationale for treating itch with inhibitors of voltage-gated sodium channels, especially NaV1.7, is as follows:
  • the propagation of electrical activity in the C fibers that sense pruritinergic stimulants requires sodium entry through voltage-gated sodium channels.
  • NaV1.7 is expressed in the C fibers and kerotinocytes in human skin (Zhao, P., et al., Pain (2008), 139: 90-105).
  • a gain of function mutation of NaV1.7 (L858F) that causes erythromelalgia also causes chronic itch (Li, Y., et al., Clinical and Experimental Dermatology (2009), 34: e313-e4).
  • Chronic itch can be alleviated with treatment by sodium channel blockers, such as the local anesthetic lidocaine (Oaklander, A. L., et al., Pain (2002), 96: 9-12; Villamil, A. G., et al., The American Journal of Medicine (2005), 118: 1160-3).
  • lidocaine was effective when administered either intravenously or topically (a Lidoderm patch).
  • Lidocaine can have multiple activities at the plasma concentrations achieved when administered systemically, but when administered topically, the plasma concentrations are only about 1 ⁇ M (Center for Drug Evaluation and Research NDA 20-612).
  • lidocaine is selective for sodium channel block and inhibits spontaneous electrical activity in C fibers and pain responses in animal models (Xiao, W. H., and Bennett, G. J. Pain (2008), 137: 218-28).
  • the types of itch or skin irritation include, but are not limited to:
  • the compounds of the invention are also useful in treating certain cancers, such as hormone sensitive cancers, such as prostate cancer (adenocarcinoma), breast cancer, ovarian cancer, testicular cancer and thyroid neoplasia, in a mammal, preferably a human.
  • hormone sensitive cancers such as prostate cancer (adenocarcinoma), breast cancer, ovarian cancer, testicular cancer and thyroid neoplasia
  • the voltage gated sodium channels have been demonstrated to be expressed in prostate and breast cancer cells. Up-regulation of neonatal NaV1.5 occurs as an integral part of the metastatic process in human breast cancer and could serve both as a novel marker of the metastatic phenotype and a therapeutic target (Clin. Cancer Res. (2005), Aug. 1; 11(15): 5381-9).
  • the present invention provides a method for treating a mammal for, or protecting a mammal from developing, a sodium channel-mediated disease, especially pain, comprising administering to the mammal, especially a human, in need thereof, a therapeutically effective amount of a compound of the invention or a pharmaceutical composition comprising a therapeutically effective amount of a compound of the invention wherein the compound modulates the activity of one or more voltage-dependent sodium channels.
  • a disease or a condition in a mammal preferably a human
  • the disease or condition is selected from the group consisting of pain, depression, cardiovascular diseases, respiratory diseases, and psychiatric diseases, and combinations thereof
  • the method comprises administering to the mammal in need thereof a therapeutically effective amount of an embodiment of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, or a pharmaceutical composition comprising a therapeutically effective amount of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, and a pharmaceutically acceptable excipient.
  • One embodiment of this embodiment is wherein the disease or condition is selected from the group consisting of acute pain, chronic pain, neuropathic pain, inflammatory pain, visceral pain, cancer pain, chemotherapy pain, trauma pain, surgical pain, post surgical pain, childbirth pain, labor pain, neurogenic bladder, ulcerative colitis, persistent pain, peripherally mediated pain, centrally mediated pain, chronic headache, migraine headache, sinus headache, tension headache, phantom limb pain, peripheral nerve injury, and combinations thereof.
  • the disease or condition is selected from the group consisting of pain associated with HIV, HIV treatment induced neuropathy, trigeminal neuralgia, post herpetic neuralgia, eudynia, heat sensitivity, tosarcoidosis, irritable bowel syndrome, Crohns disease, pain associated with multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS), diabetic neuropathy, peripheral neuropathy, arthritic, rheumatoid arthritis, osteoarthritis, atherosclerosis, paroxysmal dystonia, myasthenia syndromes, myotonia, malignant hyperthermia, cystic fibrosis, pseudoaldosteronism, rhabdomyolysis, hypothyroidism, bipolar depression, anxiety, schizophrenia, sodium channel toxin related illnesses, familial erythromelalgia, primary erythromelalgia, familial rectal pain, cancer, epilepsy, partial and general tonic seizures, restless leg
  • Another embodiment of the invention is a method of treating, but not preventing, pain in a mammal, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, or a pharmaceutical composition comprising a therapeutically effective amount of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, and a pharmaceutically acceptable excipient.
  • One embodiment of this embodiment is a method wherein the pain is selected from the group consisting of neuropathic pain, inflammatory pain, visceral pain, cancer pain, chemotherapy pain, trauma pain, surgical pain, post surgical pain, childbirth pain, labor pain, dental pain, chronic pain, persistent pain, peripherally mediated pain, centrally mediated pain, chronic headache, migraine headache, sinus headache, tension headache, phantom limb pain, peripheral nerve injury, trigeminal neuralgia, post herpetic neuralgia, eudynia, familial erythromelalgia, primary erythromelalgia, familial rectal pain or fibromyalgia, and combinations thereof.
  • Another embodiment of this embodiment is a method wherein the pain is associated with a disease or condition selected from HIV, HIV treatment induced neuropathy, heat sensitivity, tosarcoidosis, irritable bowel syndrome, Crohns disease, multiple sclerosis, amyotrophic lateral sclerosis, diabetic neuropathy, peripheral neuropathy, rheumatoid arthritis, osteoarthritis, atherosclerosis, paroxysmal dystonia, myasthenia syndromes, myotonia, malignant hyperthermia, cystic fibrosis, pseudoaldosteronism, rhabdomyolysis, hypothyroidism, bipolar depression, anxiety, schizophrenia, sodium channel toxin related illnesses, neurogenic bladder, ulcerative colitis, cancer, epilepsy, partial and general tonic seizures, restless leg syndrome, arrhythmias, ischaemic conditions caused by stroke or neural trauma, tachy arrhythmias, atrial fibrillation and ventricular fibrillation.
  • a disease or condition selected from HIV, HIV treatment
  • Another embodiment of the invention is the method of treating pain in a mammal, preferably a human, by the inhibition of ion flux through a voltage dependent sodium channel in the mammal, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount of an embodiment of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, or a pharmaceutical composition comprising a therapeutically effective amount of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, and a pharmaceutically acceptable excipient.
  • Another embodiment of the invention is the method of treating pruritus in a mammal, preferably a human, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount of an embodiment of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, or a pharmaceutical composition comprising a therapeutically effective amount of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, and a pharmaceutically acceptable excipient.
  • Another embodiment of the invention is the method of treating cancer in a mammal, preferably a human, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount of an embodiment of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, or a pharmaceutical composition comprising a therapeutically effective amount of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, and a pharmaceutically acceptable excipient.
  • Another embodiment of the invention is the method of decreasing ion flux through a voltage dependent sodium channel in a cell in a mammal, wherein the method comprises contacting the cell with an embodiment of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof.
  • Another embodiment of the invention is the method of selectively inhibiting a first voltage-gated sodium channel over a second voltage-gated sodium channel in a mammal, wherein the method comprises administering to the mammal an inhibitory amount of a compound of formula (I), or an embodiment of a compound of formula (I).
  • Another embodiment of the invention is the method of selectively inhibiting NaV1.7 in a mammal or a mammalian cell as compared to NaV1.5, wherein the method comprises administering to the mammal in need thereof an inhibitory amount of a compound of formula (I) or an embodiment of an embodiment thereof.
  • the present invention also contemplates relatedly a compound of formula I or an embodiment thereof for the use as a medicament in the treatment of such diseases and conditions.
  • the present invention also contemplates relatedly the use of a compound of formula I or an embodiment thereof for the manufacture of a medicament for the treatment of such diseases and conditions.
  • Another embodiment of the invention is a method of using the compounds of formula (I) as standards or controls in in vitro or in vivo assays in determining the efficacy of test compounds in modulating voltage-dependent sodium channels.
  • the compounds of formula (I) are isotopically-labeled by having one or more atoms therein replaced by an atom having a different atomic mass or mass number.
  • isotopically-labeled (i.e., radiolabelled) compounds of formula (I) are considered to be within the scope of this invention.
  • isotopes that can be incorporated into the compounds of formula (I) include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, sulfur, fluorine, chlorine, and iodine, such as, but not limited to, 2 H, 3 H, 11 C, 13 C, 14 C, 13 N, 15 N, 15 O, 17 O, 18 O, 31 P, 32 P, 35 S, 8 F, 36 Cl, 123 I, and 125 I, respectively.
  • isotopically-labeled compounds would be useful to help determine or measure the effectiveness of the compounds, by characterizing, for example, the site or mode of action on the sodium channels, or binding affinity to pharmacologically important site of action on the sodium channels, particularly NaV1.7.
  • isotopically-labeled compounds of formula (I), for example, those incorporating a radioactive isotope, are useful in drug and/or substrate tissue distribution studies.
  • the radioactive isotopes tritium, i.e. 3 H, and carbon-14, i.e., 14 C, are particularly useful for this purpose in view of their ease of incorporation and ready means of detection.
  • substitution with heavier isotopes such as deuterium, i.e. 2 H, may afford certain therapeutic advantages resulting from greater metabolic stability, for example, increased in vivo half-life or reduced dosage requirements, and hence may be preferred in some circumstances.
  • Isotopically-labeled compounds of formula (I) can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described in the Examples as set out below using an appropriate isotopically-labeled reagent in place of the non-labeled reagent previously employed.
  • the assessment of the compounds of the invention in mediating, especially inhibiting, the sodium channel ion flux can be determined using the assays described hereinbelow.
  • the assessment of the compounds in treating conditions and diseases in humans may be established in industry standard animal models for demonstrating the efficacy of compounds in treating pain.
  • Animal models of human neuropathic pain conditions have been developed that result in reproducible sensory deficits (allodynia, hyperalgesia, and spontaneous pain) over a sustained period of time that can be evaluated by sensory testing.
  • By establishing the degree of mechanical, chemical, and temperature induced allodynia and hyperalgesia present several physiopathological conditions observed in humans can be modeled allowing the evaluation of pharmacotherapies.
  • ectopic activity in the injured nerve corresponds to the behavioural signs of pain.
  • intravenous application of the sodium channel blocker and local anesthetic lidocaine can suppress the ectopic activity and reverse the tactile allodynia at concentrations that do not affect general behaviour and motor function (Mao, J. and Chen, L. L, Pain (2000), 87:7-17). Allometric scaling of the doses effective in these rat models, translates into doses similar to those shown to be efficacious in humans (Tanelian, D. L. and Brose, W. G., Anesthesiology (1991), 74(5):949-951).
  • Lidoderm® lidocaine applied in the form of a dermal patch
  • Lidoderm® is currently an FDA approved treatment for post-herpetic neuralgia (Devers, A. and Glaler, B. S., Clin. J. Pain (2000), 16(3):205-8).
  • the present invention readily affords many different means for identification of sodium channel modulating agents that are useful as therapeutic agents. Identification of modulators of sodium channel can be assessed using a variety of in vitro and in vivo assays, e.g., measuring current, measuring membrane potential, measuring ion flux, (e.g., sodium or guanidinium), measuring sodium concentration, measuring second messengers and transcription levels, and using e.g., voltage-sensitive dyes, radioactive tracers, and patch-clamp electrophysiology.
  • in vitro and in vivo assays e.g., measuring current, measuring membrane potential, measuring ion flux, (e.g., sodium or guanidinium), measuring sodium concentration, measuring second messengers and transcription levels, and using e.g., voltage-sensitive dyes, radioactive tracers, and patch-clamp electrophysiology.
  • One such protocol involves the screening of chemical agents for ability to modulate the activity of a sodium channel thereby identifying it as a modulating agent.
  • Throughput of test compounds is an important consideration in the choice of screening assay to be used. In some strategies, where hundreds of thousands of compounds are to be tested, it is not desirable to use low throughput means. In other cases, however, low throughput is satisfactory to identify important differences between a limited number of compounds. Often it will be necessary to combine assay types to identify specific sodium channel modulating compounds.
  • Electrophysiological assays using patch clamp techniques is accepted as a gold standard for detailed characterization of sodium channel compound interactions, and as described in Bean et al., op. cit. and Leuwer, M., et al., op. cit.
  • LTS manual low-throughput screening
  • MTS medium-throughput screening
  • HTS high-throughput screening
  • Planar electrodes are capable of achieving high-resistance, cells-attached seals followed by stable, low-noise whole-cell recordings that are comparable to conventional recordings.
  • a suitable instrument is the PatchXpress 7000A (Axon Instruments Inc, Union City, Calif.).
  • a variety of cell lines and culture techniques, which include adherent cells as well as cells growing spontaneously in suspension are ranked for seal success rate and stability.
  • Immortalized cells e.g. HEK and CHO
  • stably expressing high levels of the relevant sodium ion channel can be adapted into high-density suspension cultures.
  • assays can be selected which allow the investigator to identify compounds which block specific states of the channel, such as the open state, closed state or the resting state, or which block transition from open to closed, closed to resting or resting to open. Those skilled in the art are generally familiar with such assays.
  • Binding assays are also available. Designs include traditional radioactive filter based binding assays or the confocal based fluorescent system available from Evotec OAI group of companies (Hamburg, Germany), both of which are HTS.
  • Radioactive flux assays can also be used.
  • channels are stimulated to open with veratridine or aconitine and held in a stabilized open state with a toxin, and channel blockers are identified by their ability to prevent ion influx.
  • the assay can use radioactive 22[Na] and 14[C] guanidinium ions as tracers.
  • FlashPlate & Cytostar-T plates in living cells avoids separation steps and are suitable for HTS. Scintillation plate technology has also advanced this method to HTS suitability. Because of the functional aspects of the assay, the information content is reasonably good.
  • HTS FLIPR system membrane potential kit
  • Sodium dyes can be used to measure the rate or amount of sodium ion influx through a channel. This type of assay provides a very high information content regarding potential channel blockers. The assay is functional and would measure Na+ influx directly. CoroNa Red, SBFI and/or sodium green (Molecular Probes, Inc. Eugene Oreg.) can be used to measure Na influx; all are Na responsive dyes. They can be used in combination with the FLIPR instrument. The use of these dyes in a screen has not been previously described in the literature. Calcium dyes may also have potential in this format.
  • FRET based voltage sensors are used to measure the ability of a test compound to directly block Na influx.
  • HTS systems include the VIPRTM II FRET system (Life Technologies, or Aurora Biosciences Corporation, San Diego, Calif., a division of Vertex Pharmaceuticals, Inc.) which may be used in conjunction with FRET dyes, also available from Aurora Biosciences.
  • This assay measures sub-second responses to voltage changes. There is no requirement for a modifier of channel function.
  • the assay measures depolarization and hyperpolarizations, and provides ratiometric outputs for quantification.
  • a somewhat less expensive MTS version of this assay employs the FLEXstationTM (Molecular Devices Corporation) in conjunction with FRET dyes from Aurora Biosciences. Other methods of testing the compounds disclosed herein are also readily known and available to those skilled in the art.
  • Modulating agents so identified are then tested in a variety of in vivo models so as to determine if they alleviate pain, especially chronic pain or other conditions such as cancer and pruritus (itch) with minimal adverse events.
  • the assays described below in the Biological Assays Section are useful in assessing the biological activity of the instant compounds.
  • the efficacy of a compound of the invention is expressed by its IC50 value (“Inhibitory Concentration—50%”), which is the measure of the amount of compound required to achieve 50% inhibition of the activity of the target sodium channel over a specific time period.
  • IC50 value “Inhibitory Concentration—50%”
  • representative compounds of the present invention have demonstrated IC50's ranging from less than 100 nanomolar to less than 10 micromolar in the patch voltage clamp NaV1.7 electrophysiology assay described herein.
  • the compounds of the invention can be used in in vitro or in vivo studies as exemplary agents for comparative purposes to find other compounds also useful in treatment of, or protection from, the various diseases disclosed herein.
  • Another aspect of the invention relates to inhibiting NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.5, NaV1.6, NaV1.7, NaV1.8, or NaV1.9 activity, preferably NaV1.7 activity, in a biological sample or a mammal, preferably a human, which method comprises administering to the mammal, preferably a human, or contacting said biological sample with a compound of formula (I) or a pharmaceutical composition comprising a compound of formula (I).
  • biological sample includes, without limitation, cell cultures or extracts thereof; biopsied material obtained from a mammal or extracts thereof; and blood, saliva, urine, feces, semen, tears, or other body fluids or extracts thereof.
  • Inhibition of NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.5, NaV1.6, NaV1.7, NaV1.8, or NaV1.9 activity in a biological sample is useful for a variety of purposes that are known to one of skill in the art. Examples of such purposes include, but are not limited to, the study of sodium ion channels in biological and pathological phenomena; and the comparative evaluation of new sodium ion channel inhibitors.
  • the compounds of the invention can be used in the preparation of a medicament for the treatment of sodium channel-mediated disease or condition in a mammal.
  • the compounds of the invention may be usefully combined with one or more other compounds of the invention or one or more other therapeutic agent or as any combination thereof, in the treatment of sodium channel-mediated diseases and conditions.
  • a compound of the invention may be administered simultaneously, sequentially or separately in combination with other therapeutic agents, including, but not limited to:
  • Sodium channel-mediated diseases and conditions that may be treated and/or prevented using such combinations include but not limited to, pain, central and peripherally mediated, acute, chronic, neuropathic as well as other diseases with associated pain and other central nervous disorders such as epilepsy, anxiety, depression and bipolar disease; or cardiovascular disorders such as arrhythmias, atrial fibrillation and ventricular fibrillation; neuromuscular disorders such as restless leg syndrome and muscle paralysis or tetanus; neuroprotection against stroke, neural trauma and multiple sclerosis; and channelopathies such as erythromyalgia and familial rectal pain syndrome.
  • “combination” refers to any mixture or permutation of one or more compounds of the invention and one or more other compounds of the invention or one or more additional therapeutic agent. Unless the context makes clear otherwise, “combination” may include simultaneous or sequentially delivery of a compound of the invention with one or more therapeutic agents. Unless the context makes clear otherwise, “combination” may include dosage forms of a compound of the invention with another therapeutic agent. Unless the context makes clear otherwise, “combination” may include routes of administration of a compound of the invention with another therapeutic agent. Unless the context makes clear otherwise, “combination” may include formulations of a compound of the invention with another therapeutic agent. Dosage forms, routes of administration and pharmaceutical compositions include, but are not limited to, those described herein.
  • Lithium Bis(trimethylsilyl)amide (1.0 M, 4.8 mL, 4.8 mmol) was added dropwise to a solution of N-[(2,4-dimethoxyphenyl)methyl]thiazole-2-amine (1.0 g, 4.0 mmol) in THF (30 mL) at room temperature. After stirring for 30 minutes, a solution of 5-chloro-2,4-difluoro-benzenesulfonyl chloride (987 mg, 4.0 mmol) in THF (10 mL) was added. The reaction was stirred at room temperature for 3 hours, then concentrated.
  • Trifluoroacetic acid (0.34 mL, 4.59 mmol) was added dropwise to a solution of tert-butyl((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)carbamate (300 mg, 0.46 mmol) in DCM (1.5 mL) at room temperature. After 16 hours, the reaction was concentrated. The crude product (185 mg) was used without further purification in the subsequent step.
  • Example 2 LCMS (ESI) m/z: 434 [M+H] + .
  • Example 4 LCMS (ESI) m/z: 420 [M+H] + .
  • step 1 Following the procedure described in Example 11, step 1 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide with N-(2,4-dimethoxybenzyl)-2,4,5-trifluoro-N-(1,2,4-thiadiazol-5-yl)benzenesulfonamide, N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2,5-difluoro-N-(1,2,4-thiadiazol-5-yl)benzenesulfonamide was obtained as an off-white solid.
  • Example 25 LCMS (ESI) m/z: 440 [M+H] + .
  • Example 36 LCMS (ESI) m/z: 501.2 [M+H] + .
  • Example 77 LCMS (ESI) m/z: 501.2 [M+H] + .
  • Example 42 LCMS (ESI) m/z: 434 [M+H] + .
  • Example 43 LCMS (ESI) m/z: 434 [M+H] + .
  • tert-butyl 2-bromoacetate (3.10 mL, 21.0 mmol) was added to a mixture of 4-bromo-2-chlorophenol (4.15 g, 20.0 mmol) and cesium carbonate (7.17 g, 22.0 mmol) in N,N-dimethylformamide (10 mL). The mixture was heated at 80° C. for 18 hours, cooled down and mixed with water. The product was extracted with ethyl acetate.
  • HATU (4268 mg, 11.22 mmol) was added to a mixture of 2-(4-bromo-2-chlorophenoxy)acetic acid (2655 mg, 10.00 mmol), (1S,2S)—N 1 ,N 1 -dimethylcyclohexane-1,2-diamine (1494 mg, 10.50 mmol) and triethylamine (3.50 mL, 25.1 mmol) in 30 ml of DMF. The mixture was stirred for 4 hours, concentrated in vacuum and the residue partitioned between water and ethyl acetate. The organic extracts were washed with sat.
  • the crude product was purified by column chromatography (0-10% gradient of MeOH in DCM) to provide N-(2,4-dimethoxybenzyl)-4-((1S,2S)-2-(dimethylamino)cyclohexyl)-3-oxo-N-(thiazol-2-yl)-3,4-dihydro-2H-benzo[b][1,4]oxazine-7-sulfonamide (208 mg, 35% yield) as a yellowish solid.
  • Trifluoroacetic acid (1 ml) was added dropwise to a solution of N-[(2,4-dimethoxyphenyl)methyl]-4-[[(1S,2S)-2-(dimethylamino)cyclohexyl]amino]-3-nitro-N-thiazol-2-yl-benzenesulfonamide (64 mg, 0.11 mmol) in 1 ml of DCM.
  • the mixture was stirred for 2 hours, concentrated and the residue triturated with ethyl ether to form a precipitate. The precipitate was filtered, washed with ethyl ether and dried on air.

Abstract

or a pharmaceutically acceptable salt thereof, wherein the variables X, Y1—Y5, R1, R2, R3, R4, and Het have the meaning as described herein, and compositions containing such compounds and methods for using such compounds and compositions.

Description

    CROSS REFERENCE TO RELATED APPLICATIONS
  • This application is a continuation of U.S. patent application Ser. No. 15/799,771, filed 31 Oct. 2017, which is a continuation of international patent application number PCT/CN2017/103499, filed 26 Sep. 2017, the entire contents of the above referenced applications is hereby incorporated by reference herein this application also claims priority to international patent application number PCT/CN2016/102263, filed 17 Oct. 2016.
  • The present invention relates to organic compounds useful for therapy in a mammal, and in particular to inhibitors of sodium channel (e.g., NaV1.7) that are useful for treating sodium channel-mediated diseases or conditions, such as pain, as well as other diseases and conditions associated with the modulation of sodium channels.
  • Voltage-gated sodium channels are transmembrane proteins that initiate action potentials in nerve, muscle and other electrically excitable cells, and are a necessary component of normal sensation, emotions, thoughts and movements (Catterall, W. A., Nature (2001), Vol. 409, pp. 988-990). These channels consist of a highly processed alpha subunit that is associated with auxiliary beta subunits. The pore-forming alpha subunit is sufficient for channel function, but the kinetics and voltage dependence of channel gating are in part modified by the beta subunits (Goldin et al., Neuron (2000), Vol. 28, pp. 365-368). Electrophysiological recording, biochemical purification, and molecular cloning have identified ten different sodium channel alpha subunits and four beta subunits (Yu, F. H., et al., Sci. STKE (2004), 253; and Yu, F. H., et al., Neurosci. (2003), 20:7577-85).
  • The sodium channel family of proteins has been extensively studied and shown to be involved in a number of vital body functions. Research in this area has identified variants of the alpha subunits that result in major changes in channel function and activities, which can ultimately lead to major pathophysiological conditions. The members of this family of proteins are denoted NaV1.1 to NaV1.9.
  • NaV1.7 is a tetrodotoxin-sensitive voltage-gated sodium channel encoded by the gene SCN9A. Human NaV1.7 was first cloned from neuroendocrine cells (Klugbauer, N., et al., 1995 EMBO J., 14 (6): 1084-90.) and rat NaV1.7 was cloned from a pheochromocytoma PC12 cell line (Toledo-Aral, J. J., et al., Proc. Natl. Acad. Sci. USA (1997), 94:1527-1532) and from rat dorsal root ganglia (Sangameswaran, L., et al., (1997), J. Biol. Chem., 272 (23): 14805-9). NaV1.7 is expressed primarily in the peripheral nervous system, especially nocieptors and olfactory neurons and sympathetic neurons. The inhibition, or blocking, of NaV1.7 has been shown to result in analgesic activity. Knockout of NaV1.7 expression in a subset of sensory neurons that are predominantly nociceptive results in resistance to inflammatory pain (Nassar, et al., op. cit.). Likewise, loss of function mutations in humans results in congenital indifference to pain (CIP), in which the individuals are resistant to both inflammatory and neuropathic pain (Cox, J. J., et al., Nature (2006); 444:894-898; Goldberg, Y. P., et al., Clin. Genet. (2007); 71:311-319). Conversely, gain of function mutations in NaV1.7 have been established in two human heritable pain conditions, primary erythromelalgia and familial rectal pain, (Yang, Y., et al., J. Med. Genet. (2004), 41(3): 171-4). In addition, a single nucleotide polymorphism (R1150W) that has very subtle effects on the time- and voltage-dependence of channel gating has large effects on pain perception (Estacion, M., et al., 2009. Ann Neurol 66: 862-6; Reimann, F., et al., Proc Natl Acad Sci USA (2010), 107: 5148-53). About 10% of the patients with a variety of pain conditions have the allele conferring greater sensitivity to pain and thus might be more likely to respond to block of NaV1.7. Because NaV1.7 is expressed in both sensory and sympathetic neurons, one might expect that enhanced pain perception would be accompanied by cardiovascular abnormalities such as hypertension, but no correlation has been reported. Thus, both the CIP mutations and SNP analysis suggest that human pain responses are more sensitive to changes in NaV1.7 currents than are perturbations of autonomic function.
  • Sodium channel blockers have been shown to be useful in the treatment of pain, (see, e.g., Wood, J. N., et al., J. Neurobiol. (2004), 61(1), 55-71. Genetic and functional studies have provided evidence to support that activity of NaV1.7 as a major contributor to pain signalling in mammals. (See Hajj, et al. Nature Reviews Neuroscience; 2013, vol 14, 49-62; and Lee, et al. Cell; 2014, vol 157; 1-12). Presently, there are a limited number of effective sodium channel blockers for the treatment of pain with a minimum of adverse side effects which are currently in the clinic. Thus there remains a need for selective voltage-gated sodium channel modulators (e.g., modulators of NaV1.7) that can provide a greater therapeutic index for treatment.
    • SUMMARY
  • In one aspect the present invention provides novel compounds having sodium channel blocking activity that are useful for the treatment of pain.
  • In a first embodiment (Embodiment 1; abbreviated as “E1”) the invention provides for a compound of Formula I:
  • Figure US20200115354A1-20200416-C00002
  • or a pharmaceutically acceptable salt thereof, wherein:
      • R1, R2, R3, and R4 are each independently selected from hydrogen, C1-C6 alkyl, cyano, C3-C6 cycloalkyl, hydroxy, C1-C6 alkoxy, nitro, and halo, wherein any C1-C6 alkyl, and C3-C6 cycloalkyl is optionally substituted with one or more groups independently selected from halo, cyano, hydroxy, C1-C6 alkoxy, C1-C6 haloalkoxy, C3-C6 cycloalkyl, and phenyl; and Z is selected from —N(R5)—, —O—, —S—, —S(O)—, and —S(O)2—;
      • or
      • R1, R2, and R3 are each independently selected from hydrogen, C1-C6 alkyl, cyano, halo, and C1-C6 haloalkyl; and R4 and Z together with the benzene ring to which they are both attached form a 9- or 10-membered heterobicycle;
      • Het is 5- or 6-membered heteroaryl optionally substituted with one to three substituents each independently selected from C1-C6 alkyl, cyano, halo, and C1-C6 haloalkyl; X is selected from —N(R6)2 and —N(RT)3 + −W, and amine oxides thereof;
      • Y1 is —C(R8)2—; and Y2 is selected from —(C(R8)2)—. —N(R9)—, —O—, —C(R8)2—N(R9)—, —N(R9)—C(R8)2—, —C(R8)2—O—, and —O—C(R8)2—;
      • or
      • Y1 is selected from —N(R9)— and —O—; and Y2 is —(C(R8)2)n—;
      • n is selected from 0, 1, and 2;
      • Y3 and Y6 are each —C(R8)2—;
      • Y4 and Y5 are each —C(R8)—;
      • R5 is selected from hydrogen, C1-C6 alkyl, and C3-C6 cycloalkyl;
      • each R6 is independently selected from hydrogen, C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkanoyl, 4-7 membered heterocycle, 5-6 membered heteroaryl, and C6-C12 aryl, wherein any C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkanoyl, 4-7 membered heterocycle, 5-6 membered heteroaryl, and C6-C12 aryl is optionally substituted with one or more groups independently selected from deuterium, halo, cyano, hydroxy, C1-C6 alkyl, C1-C6 alkoxy, C1-C6 alkanoyl, C1-C6 haloalkyl, C1-C6 haloalkoxy, C3-C6 cycloalkyl, C3-C6 halocycloalkyl, —NRaRb, —C(═O)NRaRb, Rc, and phenyl, wherein any C1-C6 alkyl, C1-C6 alkoxy, and C1-C6 alkanoyl is optionally substituted with C3-C6 cycloalkyl; or two R6 groups together with the nitrogen to which they are both attached form a 4- to 10-membered heterocycle that is optionally substituted with one or more groups independently selected from deuterium, halo, cyano, hydroxy, C1-C6 alkyl, and C3-C6 cycloalkyl, which C1-C6 alkyl, and C3-C6 cycloalkyl is optionally substituted with one or more groups independently selected from hydroxy and halo;
      • each R7 is independently selected from C1-C6 alkyl; or two R7 groups together with the nitrogen to which they are both attached form a 4- to 7-membered heterocycle;
      • each R8 is independently selected from hydrogen, C1-C6 alkyl, C1-C6 haloalkyl, C3-C6 cycloalkyl, benzyl, 5-15 membered heteroaryl, C1-C6 alkoxy, C1-C6 haloalkoxy, C3-C6 cycloalkoxy, hydroxy, halo, cyano, and -L-C6-C12 aryl; wherein each C3-C6 cycloalkyl, -L-C6-C12 aryl, benzyl, 5-15 membered heteroaryl, and C1-C6 alkoxy, is optionally substituted with one to three substituents RX each independently selected from C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkoxy, cyano, halo, hydroxy, C3-C6 cycloalkoxy, C3-C6 halocycloalkyl, —S(C1-C6 alkyl), —S(O)(C1-C6 alkyl), —S(O)2(C1-C6 alkyl), —NH2—NH(C1-C6 alkyl), —N(C1-C6 alkyl)2, 4-6 membered heterocycle, and C1-C6 haloalkyl or two R8 that are on adjacent carbons taken together form a double bond; and
      • R9 is selected from hydrogen, C1-C6 alkyl and C6-C12 aryl, which C6-C12 aryl is optionally substituted with one to three substituents each independently selected from C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkoxy, cyano, halo, and C1-C6 haloalkyl; or one R8 or R9 taken together with another R8 or R9 and the atoms to which they are attached form a 3-8 membered fused, bridged or spirocyclic ring, which 3-8 membered ring is optionally substituted with one to three substituents each independently selected from C1-C6 alkyl, C1-C6 alkoxy, cyano, halo, and C1-C6 haloalkyl; and L is selected from a bond, —O—, —S—, —S(O)—, and —S(O)2—;
      • each Ra and Rb is independently selected from the group consisting of hydrogen, C1-C6 alkyl, and C1-C6 alkanoyl;
      • each Rc is independently selected from 4-7 membered heterocycle that is optionally substituted with one or more groups independently selected from halo, C1-C6 alkyl, and C1-C6 haloalkyl; and
      • W− is a counterion.
  • E2. The compound of E1 wherein:
      • R1, R2, R3, and R4 are each independently selected from hydrogen, C1-C6 alkyl, cyano, C3-C6 cycloalkyl, halo, and C1-C6 haloalkyl; and Z is selected from —N(R5)—, —O—, —S—, —S(O)—, and —S(O)2—;
      • or
      • R1, R2, and R3 are each independently selected from hydrogen, C1-C6 alkyl, cyano, halo, and C1-C6 haloalkyl; and R4 and Z together with the benzene ring to which they are both attached form a 9- or 10-membered heterobicycle;
      • Het is 5- or 6-membered heteroaryl optionally substituted with one to three substituents each independently selected from C1-C6 alkyl, cyano, halo, and C1-C6 haloalkyl;
      • X is selected from —N(R6)2 and —N(R7)3 + −W;
      • Y1 is —C(R8)2—; and Y2 is selected from —(C(R8)2)n—, —N(R9)—, —O—, —C(R8)2—N(R9)—, —N(R9)—C(R8)2—, —C(R8)2—O—, and —O—C(R8)2—;
      • or
      • Y1 is selected from —N(R9)— and —O—; and Y2 is —(C(R8)2)n—;
      • n is selected from 0, 1, and 2;
      • Y3 and Y6 are each —C(R8)2—;
      • Y4 and Y5 are each —C(R8)—;
      • R5 is selected from hydrogen, C1-C6 alkyl, and C3-C6 cycloalkyl;
      • each R6 is independently selected from hydrogen, C2-C6 alkoxyalkyl, C1-C6 alkyl, benzyl, C3-C6 cycloalkyl, C1-C6 haloalkyl, C1-C6 hydroxyalkyl, and phenyl; or two R6 groups together with the nitrogen to which they are both attached form a 4- to 7-membered heterocycle;
      • each R7 is independently selected from C1-C6 alkyl; or two R7 groups together with the nitrogen to which they are both attached form a 4- to 7-membered heterocycle;
      • each R8 is independently selected from hydrogen, C1-C6 alkyl, C1-C6 haloalkyl, C1-C6 haloalkoxy, C1-C6 alkoxy, C1-C6 hydroxyalkyl, hydroxyl, halo, cyano, benzyl and phenyl; wherein each benzyl or phenyl is optionally substituted with one to three substituents each independently selected from C1-C6 alkyl, cyano, halo, and C1-C6 haloalkyl or two R8 that are on adjacent carbons taken together form a double bond; and R9 is selected from hydrogen and C1-C6 alkyl; or one R8 or R9 taken together with another R8 or R9 and the atoms to which they are attached form a 3-8 membered fused, bridged or spirocyclic ring; and
      • W is a counterion; or a pharmaceutically acceptable salt thereof.
  • E3. The compound of E1 or E2, which is a compound of formula Ia:
  • Figure US20200115354A1-20200416-C00003
  • wherein R4 and Z together with the benzene ring to which they are both attached form a 9- or 10-membered heterobicycle, or a pharmaceutically acceptable salt thereof.
  • E4. The compound of E1 or E2, which is a compound of formula Ib:
  • Figure US20200115354A1-20200416-C00004
  • or a pharmaceutically acceptable salt thereof.
  • E5. The compound of E1 or E2, which is a compound of formula Ic:
  • Figure US20200115354A1-20200416-C00005
  • or a pharmaceutically acceptable salt thereof.
  • E6. The compound of E1, E2, E3, E4, or E5, wherein one of Y1 and Y2 is —CH(R8)— and the other of Y1 and Y2 is —CH2—; R8 is not H; Y3 and Y6 are each —CH2—; and Y4 and Y5 are each —CH—; or a pharmaceutically acceptable salt thereof.
  • E7. The compound of E1 or E2, which is a compound of formula Id:
  • Figure US20200115354A1-20200416-C00006
  • or a pharmaceutically acceptable salt thereof.
  • E8. The compound of E1 or E2, which is a compound of formula Ie:
  • Figure US20200115354A1-20200416-C00007
  • or a pharmaceutically acceptable salt thereof.
  • E9. The compound of E1 or E2, which is a compound of formula If:
  • Figure US20200115354A1-20200416-C00008
  • or a pharmaceutically acceptable salt thereof.
  • E10. The compound of E1 or E2, which is a compound of formula Ig:
  • Figure US20200115354A1-20200416-C00009
  • wherein --- is a single bond or a double bond; or a pharmaceutically acceptable salt thereof.
  • E11. The compound of E1 or E2, which is a compound of formula Ih:
  • Figure US20200115354A1-20200416-C00010
  • or a pharmaceutically acceptable salt thereof.
  • E12. The compound of E6, E7, E8, E9, E10, or E11, wherein R8 is trifluoromethyl or phenyl that is optionally substituted with one to three substituents each independently selected from C1-C6 alkyl, cyano, halo, and C1-C6 haloalkyl; or a pharmaceutically acceptable salt thereof.
  • E13. The compound of E6, E7, E8, E9, E10, or E11, wherein R8 is trifluoromethyl or 3-trifluoromethylphenyl; or a pharmaceutically acceptable salt thereof.
  • E14. The compound of E6, E7, E8, E9, E10, or E11, wherein R8 is phenyl that is optionally substituted with one to three substituents RX each independently selected from the group consisting of C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkoxy, cyano, halo, hydroxy, C3-C6 cycloalkoxy, C3-C6 halocycloalkyl, —S(C1-C6 alkyl), —S(O)(C1-C6 alkyl), —S(O)2(C1-C6 alkyl), —NH2—NH(C1-C6 alkyl), —N(C1-C6 alkyl)2, 4-6 membered heterocycle, and C1-C6 haloalkyl; or a pharmaceutically acceptable salt thereof.
  • E15. The compound of E6, E7, E8, E9, E10, or E11, wherein R8 is phenyl that is optionally substituted with one to three substituents RX each independently selected from the group consisting of C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkoxy, cyano, halo, and C1-C6 haloalkyl; or a pharmaceutically acceptable salt thereof.
  • E16. The compound of E6, E7, E8, E9, E10, or E11, wherein R8 is phenyl that is optionally substituted with one to three substituents RX each independently selected from the group consisting of F, Cl, CN, C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkyl, and C1-C6 haloalkoxy; or a pharmaceutically acceptable salt thereof.
  • E17. The compound of E6, E7, E8, E9, E10, or E11, wherein R8 is phenyl that is optionally substituted with one to three substituents RX each independently selected from the group consisting of fluoro, chloro, trifluoromethyl, propyl, cyclopropyl, and trifluoromethoxy; or a pharmaceutically acceptable salt thereof.
  • E18. The compound of E1 or E2, which is a compound of formula (Ik):
  • Figure US20200115354A1-20200416-C00011
  • wherein:
      • n is 1, 2, or 3; and
      • each RX is independently selected from the group consisting of C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkoxy, cyano, halo, hydroxy, C3-C6 cycloalkoxy, C3-C6 halocycloalkyl, —S(C1-C6 alkyl), —S(O)(C1-C6 alkyl), —S(O)2(C1-C6 alkyl), —NH2—NH(C1-C6 alkyl), —N(C1-C6 alkyl)2, 4-6 membered heterocycle, and C1-C6 haloalkyl;
      • or a pharmaceutically acceptable salt thereof.
  • E19. The compound of E1 or E2, which is a compound of formula (Im):
  • Figure US20200115354A1-20200416-C00012
  • wherein:
      • n is 1, 2, or 3; and
      • each RX is independently selected from the group consisting of C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkoxy, cyano, halo, hydroxy, C3-C6 cycloalkoxy, C3-C6 halocycloalkyl, —S(C1-C6 alkyl), —S(O)(C1-C6 alkyl), —S(O)2(C1-C6 alkyl), —NH2—NH(C1-C6 alkyl), —N(C1-C6 alkyl)2, 4-6 membered heterocycle, and C1-C6 haloalkyl;
      • or a pharmaceutically acceptable salt thereof.
  • E20. The compound of E1 or E2, which is a compound of formula (In):
  • Figure US20200115354A1-20200416-C00013
  • wherein:
      • n is 1, 2, or 3; and
      • each RX is independently selected from the group consisting of C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkoxy, cyano, halo, hydroxy, C3-C6 cycloalkoxy, C3-C6 halocycloalkyl, —S(C1-C6 alkyl), —S(O)(C1-C6 alkyl), —S(O)2(C1-C6 alkyl), —NH2—NH(C1-C6 alkyl), —N(C1-C6 alkyl)2, 4-6 membered heterocycle, and C1-C6 haloalkyl; or a pharmaceutically acceptable salt thereof.
  • E21. The compound of E1 or E2, which is a compound of formula (Ip):
  • Figure US20200115354A1-20200416-C00014
  • wherein:
      • n is 1, 2, or 3; and
      • each RX is independently selected from the group consisting of C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkoxy, cyano, halo, hydroxy, C3-C6 cycloalkoxy, C3-C6 halocycloalkyl, —S(C1-C6 alkyl), —S(O)(C1-C6 alkyl), —S(O)2(C1-C6 alkyl), —NH2—NH(C1-C6 alkyl), —N(C1-C6 alkyl)2, 4-6 membered heterocycle, and C1-C6 haloalkyl; or a pharmaceutically acceptable salt thereof
  • E22. The compound of E1 or E2, which is a compound of formula (Ir):
  • Figure US20200115354A1-20200416-C00015
  • wherein:
      • RX is selected from the group consisting of C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkoxy, cyano, halo, hydroxy, C3-C6 cycloalkoxy, C3-C6 halocycloalkyl, —S(C1-C6 alkyl), —S(O)(C1-C6 alkyl), —S(O)2(C1-C6 alkyl), —NH2—NH(C1-C6 alkyl), —N(C1-C6 alkyl)2, 4-6 membered heterocycle, and C1-C6 haloalkyl; or a pharmaceutically acceptable salt thereof
  • E23. The compound of E1 or E2, which is a compound of formula (Is):
  • Figure US20200115354A1-20200416-C00016
  • wherein:
      • RX is selected from the group consisting of C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkoxy, cyano, halo, hydroxy, C3-C6 cycloalkoxy, C3-C6 halocycloalkyl, —S(C1-C6 alkyl), —S(O)(C1-C6 alkyl), —S(O)2(C1-C6 alkyl), —NH2—NH(C1-C6 alkyl), —N(C1-C6 alkyl)2, 4-6 membered heterocycle, and C1-C6 haloalkyl; or a pharmaceutically acceptable salt thereof.
  • E24. The compound of E18, or E19, E20, E21, E22, or E23, wherein each RX is independently selected from the group consisting of C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkoxy, cyano, halo, and C1-C6 haloalkyl; or a pharmaceutically acceptable salt thereof.
  • E25. The compound of E18, or E19, E20, E21, E22, or E23, wherein each RX is independently selected from the group consisting of F, Cl, CN, C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkyl, and C1-C6 haloalkoxy; or a pharmaceutically acceptable salt thereof.
  • E26. The compound of E18, or E19, E20, E21, E22, or E23, wherein each RX is independently selected from the group consisting of fluoro, chloro, trifluoromethyl, propyl, cyclopropyl, and trifluoromethoxy; or a pharmaceutically acceptable salt thereof.
  • E27. The compound of E18, or E19, E20, E21, E22, or E23, wherein each RX is independently selected from F and CF3; or a pharmaceutically acceptable salt thereof.
  • E28. The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, or E17, wherein X is —N(R6)2 and each R6 is independently selected from hydrogen, C2-C6 alkoxyalkyl, C1-C6 alkyl, benzyl, C3-C6 cycloalkyl, C1-C6 haloalkyl, and C1-C6 hydroxyalkyl; or a pharmaceutically acceptable salt thereof.
  • E29. The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, or E17, wherein X is —N(R6)2 and two R6 groups together with the nitrogen to which they are both attached form a 4- to 7-membered heterocycle; or a pharmaceutically acceptable salt thereof.
  • E30. The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, or E17, wherein X is selected from the group consisting of: —NH2, —NHMe, —N(Me)2, —NHEt, —NMeEt, —N+(Me)3,
  • Figure US20200115354A1-20200416-C00017
  • or a pharmaceutically acceptable salt thereof.
  • E31. The compound of E1, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, or E17, wherein X is selected from the group consisting of:
  • Figure US20200115354A1-20200416-C00018
    Figure US20200115354A1-20200416-C00019
    Figure US20200115354A1-20200416-C00020
    Figure US20200115354A1-20200416-C00021
    Figure US20200115354A1-20200416-C00022
  • or a pharmaceutically acceptable salt thereof.
  • E32. The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, or E31, wherein R is H; or a pharmaceutically acceptable salt thereof.
  • E33. The compound of The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, or E32, wherein R2 is selected from hydrogen and halo; or a pharmaceutically acceptable salt thereof.
  • E34. The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, or E32, wherein R2 is selected from hydrogen and fluoro; or a pharmaceutically acceptable salt thereof.
  • E35. The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, E32, E33, or E34, wherein R3 is hydrogen; or a pharmaceutically acceptable salt thereof.
  • E36. The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, E32, E33, E34, or E35, wherein R4 is selected from fluoro, chloro, bromo, cyclopropyl, and trifluoromethyl; or a pharmaceutically acceptable salt thereof.
  • E37. The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, E32, E33, E34, or E35, wherein R4 is selected from hydrogen, fluoro, chloro, bromo, cyclopropyl, cyclobutyl, hydroxy, methoxy, and trifluoromethyl; or a pharmaceutically acceptable salt thereof.
  • E38. The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, E32, E33, E34, or E35, wherein R4 is chloro; or a pharmaceutically acceptable salt thereof.
  • E39. The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, E32, E33, E34, E35, E36, E37, or E38, wherein Het is a 5-membered heteroaryl optionally substituted with one to three substituents each independently selected from C1-C6 alkyl, cyano, halo, and C1-C6 haloalkyl; or a pharmaceutically acceptable salt thereof.
  • E40. The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, E32, E33, E34, E35, E36, E37, or E38, wherein Het is a 6-membered heteroaryl optionally substituted with one to three substituents each independently selected from C1-C6 alkyl, cyano, halo, and C1-C6 haloalkyl; or a pharmaceutically acceptable salt thereof.
  • E41. The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, E32, E33, E34, E35, E36, E37, or E38, wherein Het is selected from the group consisting of: thiazole, pyrimidine, oxazole, and thiadiazole, each optionally substituted with 1, 2, or 3 substituents independently selected from the group consisting of C1-C6 alkyl, cyano, halo, and C1-C6 haloalkyl; or a pharmaceutically acceptable salt thereof.
  • E42. The compound of E1, E2, E3, E4, E5, E6, E7, E8, E9, E10, E11, E12, E13, E14, E15, E16, E17, E18, E19, E20, E21, E22, E23, E24, E25, E26, E27, E28, E29, E30, E31, E32, E33, E34, E35, E36, E37, or E38, wherein Het is selected from the group consisting of: 1,3-thiazol-2-yl, pyrimidin-4-yl, e, 1,2-oxazol-3-yl, and 1,2,4-thiadiazol-5-yl; or a pharmaceutically acceptable salt thereof.
  • E43. The compound of E1 or E2, wherein the group
  • Figure US20200115354A1-20200416-C00023
  • is selected from the group consisting of:
  • Figure US20200115354A1-20200416-C00024
    Figure US20200115354A1-20200416-C00025
  • or a pharmaceutically acceptable salt thereof
  • E44. The compound of E1, wherein the group
  • Figure US20200115354A1-20200416-C00026
  • is selected from the group consisting of:
  • Figure US20200115354A1-20200416-C00027
    Figure US20200115354A1-20200416-C00028
    Figure US20200115354A1-20200416-C00029
    Figure US20200115354A1-20200416-C00030
    Figure US20200115354A1-20200416-C00031
    Figure US20200115354A1-20200416-C00032
    Figure US20200115354A1-20200416-C00033
    Figure US20200115354A1-20200416-C00034
    Figure US20200115354A1-20200416-C00035
    Figure US20200115354A1-20200416-C00036
    Figure US20200115354A1-20200416-C00037
    Figure US20200115354A1-20200416-C00038
    Figure US20200115354A1-20200416-C00039
    Figure US20200115354A1-20200416-C00040
    Figure US20200115354A1-20200416-C00041
    Figure US20200115354A1-20200416-C00042
  • or a pharmaceutically acceptable salt thereof.
  • E45. The compound of E1, which is selected from the group consisting of:
  • Figure US20200115354A1-20200416-C00043
    Figure US20200115354A1-20200416-C00044
    Figure US20200115354A1-20200416-C00045
    Figure US20200115354A1-20200416-C00046
    Figure US20200115354A1-20200416-C00047
  • or a pharmaceutically acceptable salt thereof.
  • E46. The compound of E1, which is selected from the group consisting of:
  • Figure US20200115354A1-20200416-C00048
    Figure US20200115354A1-20200416-C00049
    Figure US20200115354A1-20200416-C00050
    Figure US20200115354A1-20200416-C00051
  • or a pharmaceutically acceptable salt thereof.
  • E47. The compound of E1, which is selected from the group consisting of:
  • Figure US20200115354A1-20200416-C00052
    Figure US20200115354A1-20200416-C00053
    Figure US20200115354A1-20200416-C00054
    Figure US20200115354A1-20200416-C00055
    Figure US20200115354A1-20200416-C00056
    Figure US20200115354A1-20200416-C00057
    Figure US20200115354A1-20200416-C00058
    Figure US20200115354A1-20200416-C00059
    Figure US20200115354A1-20200416-C00060
    Figure US20200115354A1-20200416-C00061
    Figure US20200115354A1-20200416-C00062
    Figure US20200115354A1-20200416-C00063
    Figure US20200115354A1-20200416-C00064
    Figure US20200115354A1-20200416-C00065
    Figure US20200115354A1-20200416-C00066
    Figure US20200115354A1-20200416-C00067
    Figure US20200115354A1-20200416-C00068
    Figure US20200115354A1-20200416-C00069
    Figure US20200115354A1-20200416-C00070
    Figure US20200115354A1-20200416-C00071
    Figure US20200115354A1-20200416-C00072
    Figure US20200115354A1-20200416-C00073
    Figure US20200115354A1-20200416-C00074
    Figure US20200115354A1-20200416-C00075
    Figure US20200115354A1-20200416-C00076
    Figure US20200115354A1-20200416-C00077
    Figure US20200115354A1-20200416-C00078
    Figure US20200115354A1-20200416-C00079
    Figure US20200115354A1-20200416-C00080
    Figure US20200115354A1-20200416-C00081
    Figure US20200115354A1-20200416-C00082
    Figure US20200115354A1-20200416-C00083
    Figure US20200115354A1-20200416-C00084
    Figure US20200115354A1-20200416-C00085
    Figure US20200115354A1-20200416-C00086
    Figure US20200115354A1-20200416-C00087
    Figure US20200115354A1-20200416-C00088
    Figure US20200115354A1-20200416-C00089
    Figure US20200115354A1-20200416-C00090
    Figure US20200115354A1-20200416-C00091
    Figure US20200115354A1-20200416-C00092
    Figure US20200115354A1-20200416-C00093
  • or a pharmaceutically acceptable salt thereof.
  • E48. A compound of formula (Id′) of E1 or E2:
  • Figure US20200115354A1-20200416-C00094
  • or a pharmaceutically acceptable salt thereof.
  • E49. A compound of formula (Ie′) of E1 or E2:
  • Figure US20200115354A1-20200416-C00095
  • or a pharmaceutically acceptable salt thereof.
  • E50. A compound of formula (If′) of E1 or E2:
  • Figure US20200115354A1-20200416-C00096
  • or a pharmaceutically acceptable salt thereof.
  • E51. A compound of formula (Ig′) of E1 or E2:
  • Figure US20200115354A1-20200416-C00097
  • wherein --- is a single bond or a double bond; or a pharmaceutically acceptable salt thereof.
  • E52. A compound of formula (Ih′) of E1 or E2:
  • Figure US20200115354A1-20200416-C00098
  • or a pharmaceutically acceptable salt thereof.
  • E53. A compound of formula of E1 or E2 selected from:
  • Figure US20200115354A1-20200416-C00099
  • or a pharmaceutically acceptable salt thereof.
  • E54. A compound of E1 or E2 selected from:
  • Figure US20200115354A1-20200416-C00100
    Figure US20200115354A1-20200416-C00101
  • or a pharmaceutically acceptable salt thereof.
  • E55. A compound of E1 or E2 selected from:
  • Figure US20200115354A1-20200416-C00102
    Figure US20200115354A1-20200416-C00103
  • or a pharmaceutically acceptable salt thereof.
  • E56. The compound of E1, E2, E3, E4, E5, E28, E29, E30, E31, E32, E33, E34, E35, E36, E37, E38, E39, E40, E41, or E42, wherein R8 or R9 taken together with another R8 or R9 and the atoms to which they are attached form a 3-8 membered fused ring, which is a benzo ring.
  • In another aspect the present invention provides for a pharmaceutical composition comprising a compound of formula I or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.
  • In another aspect the present invention provides for a method of treating a disease or condition in a mammal selected from the group consisting of pain, depression, cardiovascular diseases, respiratory diseases, and psychiatric diseases, and combinations thereof, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount of a compound of formula I, or a pharmaceutically acceptable salt thereof. In another aspect of the present invention said disease or condition is selected from the group consisting of neuropathic pain, inflammatory pain, visceral pain, cancer pain, chemotherapy pain, trauma pain, surgical pain, post-surgical pain, childbirth pain, labor pain, neurogenic bladder, ulcerative colitis, chronic pain, persistent pain, peripherally mediated pain, centrally mediated pain, chronic headache, migraine headache, sinus headache, tension headache, phantom limb pain, dental pain, peripheral nerve injury or a combination thereof. In another aspect of the present invention said disease or condition is selected from the group consisting of pain associated with HIV, HIV treatment induced neuropathy, trigeminal neuralgia, post-herpetic neuralgia, eudynia, heat sensitivity, tosarcoidosis, irritable bowel syndrome, Crohns disease, pain associated with multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS), diabetic neuropathy, peripheral neuropathy, arthritis, rheumatoid arthritis, osteoarthritis, atherosclerosis, paroxysmal dystonia, myasthenia syndromes, myotonia, malignant hyperthermia, cystic fibrosis, pseudoaldosteronism, rhabdomyolysis, hypothyroidism, bipolar depression, anxiety, schizophrenia, sodium channel toxin related illnesses, familial erythromelalgia, primary erythromelalgia, familial rectal pain, cancer, epilepsy, partial and general tonic seizures, restless leg syndrome, arrhythmias, fibromyalgia, neuroprotection under ischaemic conditions cause by stroke or neural trauma, tach-arrhythmias, atrial fibrillation and ventricular fibrillation.
  • In another aspect the present invention provides for a method of treating pain in a mammal by the inhibition of ion flux through a voltage-dependent sodium channel in the mammal, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount of a compound of formula I, or a pharmaceutically acceptable salt thereof.
  • In another aspect the present invention provides for a method of decreasing ion flux through a voltage-dependent sodium channel in a cell in a mammal, wherein the method comprises contacting the cell with a compound of formula I, or a pharmaceutically acceptable salt thereof.
  • In another aspect the present invention provides for a method of treating pruritus in a mammal, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount of a compound of formula I, or a pharmaceutically acceptable salt thereof.
  • In another aspect the present invention provides for a method of treating cancer in a mammal, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount a compound of formula I, or a pharmaceutically acceptable salt thereof.
  • In another aspect the present invention provides for a method of treating, but not preventing, pain in a mammal, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount of a compound of formula I, or a pharmaceutically acceptable salt thereof.
  • In another aspect of the present invention the pain is selected from the group consisting of neuropathic pain, inflammatory pain, visceral pain, cancer pain, chemotherapy pain, trauma pain, surgical pain, post-surgical pain, childbirth pain, labor pain, neurogenic bladder, ulcerative colitis, chronic pain, persistent pain, peripherally mediated pain, centrally mediated pain, chronic headache, migraine headache, sinus headache, tension headache, phantom limb pain, dental pain, peripheral nerve injury or a combination thereof. In another aspect the present invention the pain is associated with a disease or condition selected from the group consisting of HIV, HIV treatment induced neuropathy, trigeminal neuralgia, post-herpetic neuralgia, eudynia, heat sensitivity, tosarcoidosis, irritable bowel syndrome, Crohns disease, pain associated with multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS), diabetic neuropathy, peripheral neuropathy, arthritis, rheumatoid arthritis, osteoarthritis, atherosclerosis, paroxysmal dystonia, myasthenia syndromes, myotonia, malignant hyperthermia, cystic fibrosis, pseudoaldosteronism, rhabdomyolysis, hypothyroidism, bipolar depression, anxiety, schizophrenia, sodium channel toxin related illnesses, familial erythromelalgia, primary erythromelalgia, familial rectal pain, cancer, epilepsy, partial and general tonic seizures, restless leg syndrome, arrhythmias, fibromyalgia, neuroprotection under ischaemic conditions cause by stroke or neural trauma, tach-arrhythmias, atrial fibrillation and ventricular fibrillation.
  • In another aspect the present invention provides for a method for the treatment or prophylaxis of pain, depression, cardiovascular disease, respiratory disease, or psychiatric disease, or a combinations thereof, in an animal which method comprises administering an effective amount of a compound of formula I, or a pharmaceutically acceptable salt thereof.
  • In another aspect the present invention provides for a compound of formula I, or a pharmaceutically acceptable salt thereof for the use as a medicament for the treatment of diseases and disorders selected from the group consisting of pain, depression, cardiovascular diseases, respiratory diseases, and psychiatric diseases, or a combination thereof.
  • In another aspect the present invention provides for the use of a compound of formula I, or a pharmaceutically acceptable salt thereof for the manufacture of a medicament for the treatment of diseases and disorders selected from the group consisting of pain, depression, cardiovascular diseases, respiratory diseases, and psychiatric diseases, or a combination thereof.
  • In another aspect the present invention provides for the invention as described herein.
    • DETAILED DESCRIPTION Definitions
  • As used herein, the term “alkyl”, by itself or as part of another substituent, means, unless otherwise stated, a straight or branched chain hydrocarbon radical, having the number of carbon atoms designated (i.e., C1-8 means one to eight carbons). Examples of alkyl groups include methyl, ethyl, n-propyl, iso-propyl, n-butyl, t-butyl, iso-butyl, sec-butyl, n-pentyl, n-hexyl, n-heptyl, n-octyl, and the like.
  • The terms “alkoxy,” “alkylamino” and “alkylthio”, are used in their conventional sense, and refer to those alkyl groups attached to the remainder of the molecule via an oxygen atom (“oxy”), an amino group (“amino”) or thio group. Additionally, for dialkylamino groups, the alkyl portions can be the same or different.
  • The terms “halo” by itself or as part of another substituent, mean, unless otherwise stated, a fluorine, chlorine, bromine, or iodine atom.
  • The term “haloalkyl” refers to an alkyl that is substituted with one or more (e.g. 1, 2, 3, 4, 5, or 6) halo groups. For example the term includes an alkyl group having 1-6 carbon atoms that is substituted with one or more halo groups. Non-limiting examples of the term C1-C6 haloalkyl include fluoromethyl, difluoromethyl, trifluoromethyl, chloromethyl, and 2,2,2-trifluoroethyl.
  • The term “halocycloalkyl” refers to a cycloalkyl that is substituted with one or more (e.g. 1, 2, 3, 4, 5, or 6) halo groups. For example the term includes a cycloalkyl group having 3-6 carbon atoms that is substituted with one or more halo groups. Non-limiting examples of the term C1-C6 halocycloalkyl include 1-fluorocyclopropyl.
  • The term “hydroxyalkyl” refers to an alkyl that is substituted with one or more (e.g. 1, 2, 3, 4, 5, or 6) hydroxy groups. For example the term includes C1-C6 hydroxyalkyl, which is an alkyl group having 1-6 carbon atoms that is substituted with one or more hydroxy groups. Non-limiting examples of the term C1-C6 hydroxyalkyl include hydroxymethyl, 2-hydroxyethyl, and 3-hydroxypropyl.
  • The term “alkoxyalkyl” refers to an alkyl that is substituted with one or more (e.g. 1, 2, 3, 4, 5, or 6) alkoxy (alkyl-O—) groups. For example the term C2-C6 alkoxyalkyl refers to an alkyl that is substituted with one or more alkoxy groups, wherein the total number of carbons is 2, 3, 4, 5, or 6. Non-limiting examples of the term C2-C6 alkoxyalkyl include methoxymethyl, 2-methoxyethyl, 2-ethoxyethyl, 3-methoxypropyl, and 3-ethoxypropyl.
  • The term “aryl” as used herein refers to a single all carbon aromatic ring or a multiple condensed all carbon ring system wherein at least one of the rings is aromatic. For example, in certain embodiments, an aryl group has 6 to 20 carbon atoms, 6 to 14 carbon atoms, or 6 to 12 carbon atoms. Aryl includes a phenyl radical. Aryl also includes multiple condensed ring systems (e.g., ring systems comprising 2, 3 or 4 rings) having about 9 to 20 carbon atoms in which at least one ring is aromatic and wherein the other rings may be aromatic or not aromatic (i.e., carbocycle). Such multiple condensed ring systems are optionally substituted with one or more (e.g., 1, 2 or 3) oxo groups on any carbocycle portion of the multiple condensed ring system. The rings of the multiple condensed ring system can be connected to each other via fused, spiro and bridged bonds when allowed by valency requirements. It is to be understood that the point of attachment of a multiple condensed ring system, as defined above, can be at any position of the ring system including an aromatic or a carbocycle portion of the ring. Non-limiting examples of aryl groups include, but are not limited to, phenyl, indenyl, naphthyl, 1,2,3,4-tetrahydronaphthyl, anthracenyl, and the like.
  • The term “carbocycle” or “carbocyclyl” refers to a single saturated (i.e., cycloalkyl) or a single partially unsaturated (e.g., cycloalkenyl, cycloalkadienyl, etc.) all carbon ring having 3 to 7 carbon atoms (i.e., (C3-C7)carbocycle). The term “carbocycle” or “carbocyclyl” also includes multiple condensed, saturated and partially unsaturated all carbon ring systems (e.g., ring systems comprising 2, 3 or 4 carbocyclic rings). Accordingly, carbocycle includes multicyclic carbocyles such as a bicyclic carbocycles (e.g., bicyclic carbocycles having about 6 to 20 or 6 to 12 carbon atoms such as bicyclo[3.1.0]hexane and bicyclo[2.1.1]hexane), and polycyclic carbocycles (e.g tricyclic and tetracyclic carbocycles with up to about 20 carbon atoms). The rings of the multiple condensed ring system can be connected to each other via fused, spiro and bridged bonds when allowed by valency requirements. For example, multicyclic carbocyles can be connected to each other via a single carbon atom to form a spiro connection (e.g., spiropentane, spiro[4,5]decane, etc), via two adjacent carbon atoms to form a fused connection (e.g., carbocycles such as decahydronaphthalene, norsabinane, norcarane) or via two non-adjacent carbon atoms to form a bridged connection (e.g., norbornane, bicyclo[2.2.2]octane, etc). The “carbocycle” or “carbocyclyl” can also be optionally substituted with one or more (e.g., 1, 2 or 3) oxo groups. In one embodiment the term carbocycle includes a C3-12 carbocycle. In one embodiment the term carbocycle includes a C3-8 carbocycle. In one embodiment the term carbocycle includes a C3-6 carbocycle. In one embodiment the term carbocycle includes a C3-5 carbocycle. Non-limiting examples of carbocycles include cyclopropyl, cyclobutyl, cyclopentyl, 1-cyclopent-1-enyl, 1-cyclopent-2-enyl, 1-cyclopent-3-enyl, cyclohexyl, 1-cyclohex-1-enyl, 1-cyclohex-2-enyl, bicyclo[2.2.1]heptane, pinane, adamantane, norborene, spirocyclic C5-12 alkane, and 1-cyclohex-3-enyl. In one embodiment the term “cycloalkyl” refers to a single saturated all carbon ring having 3 to 8 carbon atoms. Non-limiting examples of carbocycles include cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl.
  • The term “heteroaryl” as used herein refers to a single aromatic ring that has at least one atom other than carbon in the ring, wherein the atom is selected from the group consisting of oxygen, nitrogen and sulfur; “heteroaryl” also includes multiple condensed ring systems that have at least one such aromatic ring, which multiple condensed ring systems are further described below. Thus, “heteroaryl” includes single aromatic rings of from about 1 to 6 carbon atoms and about 1-4 heteroatoms selected from the group consisting of oxygen, nitrogen and sulfur. The sulfur and nitrogen atoms may also be present in an oxidized form provided the ring is aromatic. Exemplary heteroaryl ring systems include but are not limited to pyridyl, pyrimidinyl, oxazolyl or furyl. “Heteroaryl” also includes multiple condensed ring systems (e.g., ring systems comprising 2, 3 or 4 rings) wherein a heteroaryl group, as defined above, is condensed with one or more rings selected from heteroaryls (to form for example a naphthyridinyl such as 1,8-naphthyridinyl), heterocycles, (to form for example a 1,2,3,4-tetrahydronaphthyridinyl such as 1,2,3,4-tetrahydro-1,8-naphthyridinyl), carbocycles (to form for example 5,6,7,8-tetrahydroquinolyl) and aryls (to form for example indazolyl) to form the multiple condensed ring system. Thus, a heteroaryl (a single aromatic ring or multiple condensed ring system) has about 1-20 carbon atoms and about 1-6 heteroatoms within the heteroaryl ring. A heteroaryl (a single aromatic ring or multiple condensed ring system) can also have about 5 to 20 or about 5 to 15 or about 5 to 10 members within the heteroaryl ring. Such multiple condensed ring systems may be optionally substituted with one or more (e.g., 1, 2, 3 or 4) oxo groups on the carbocycle or heterocycle portions of the condensed ring. The rings of the multiple condensed ring system can be connected to each other via fused, spiro and bridged bonds when allowed by valency requirements. It is to be understood that the individual rings of the multiple condensed ring system may be connected in any order relative to one another. It is also to be understood that the point of attachment of a multiple condensed ring system (as defined above for a heteroaryl) can be at any position of the multiple condensed ring system including a heteroaryl, heterocycle, aryl or carbocycle portion of the multiple condensed ring system. It is also to be understood that the point of attachment for a heteroaryl or heteroaryl multiple condensed ring system can be at any suitable atom of the heteroaryl or heteroaryl multiple condensed ring system including a carbon atom and a heteroatom (e.g., a nitrogen). Exemplary heteroaryls include but are not limited to pyridyl, pyrrolyl, pyrazinyl, pyrimidinyl, pyridazinyl, pyrazolyl, thienyl, indolyl, imidazolyl, oxazolyl, isoxazolyl, thiazolyl, furyl, oxadiazolyl, thiadiazolyl, quinolyl, isoquinolyl, benzothiazolyl, benzoxazolyl, indazolyl, quinoxalyl, quinazolyl, 5,6,7,8-tetrahydroisoquinolinyl benzofuranyl, benzimidazolyl, thianaphthenyl, pyrrolo[2,3-b]pyridinyl, quinazolinyl-4(3H)-one, triazolyl, 4,5,6,7-tetrahydro-1H-indazole and 3b,4,4a,5-tetrahydro-1H-cyclopropa[3,4]cyclo-penta[1,2-c]pyrazole. In one embodiment the term “heteroaryl” refers to a single aromatic ring containing at least one heteroatom. For example, the term includes 5-membered and 6-membered monocyclic aromatic rings that include one or more heteroatoms. Non-limiting examples of heteroaryl include but are not limited to pyridyl, furyl, thiazole, pyrimidine, oxazole, and thiadiazole.
  • The term “heterocyclyl” or “heterocycle” as used herein refers to a single saturated or partially unsaturated ring that has at least one atom other than carbon in the ring, wherein the atom is selected from the group consisting of oxygen, nitrogen and sulfur; the term also includes multiple condensed ring systems that have at least one such saturated or partially unsaturated ring, which multiple condensed ring systems are further described below. Thus, the term includes single saturated or partially unsaturated rings (e.g., 3, 4, 5, 6 or 7-membered rings) from about 1 to 6 carbon atoms and from about 1 to 3 heteroatoms selected from the group consisting of oxygen, nitrogen and sulfur in the ring. The ring may be substituted with one or more (e.g., 1, 2 or 3) oxo groups and the sulfur and nitrogen atoms may also be present in their oxidized forms. Exemplary heterocycles include but are not limited to azetidinyl, tetrahydrofuranyl and piperidinyl. The term “heterocycle” also includes multiple condensed ring systems (e.g., ring systems comprising 2, 3 or 4 rings) wherein a single heterocycle ring (as defined above) can be condensed with one or more groups selected from heterocycles (to form for example a 1,8-decahydronapthyridinyl), carbocycles (to form for example a decahydroquinolyl) and aryls to form the multiple condensed ring system. Thus, a heterocycle (a single saturated or single partially unsaturated ring or multiple condensed ring system) has about 2-20 carbon atoms and 1-6 heteroatoms within the heterocycle ring. Such multiple condensed ring systems may be optionally substituted with one or more (e.g., 1, 2, 3 or 4) oxo groups on the carbocycle or heterocycle portions of the multiple condensed ring. The rings of the multiple condensed ring system can be connected to each other via fused, spiro and bridged bonds when allowed by valency requirements. It is to be understood that the individual rings of the multiple condensed ring system may be connected in any order relative to one another. Accordingly, a heterocycle (a single saturated or single partially unsaturated ring or multiple condensed ring system) has about 3-20 atoms including about 1-6 heteroatoms within the heterocycle ring system. It is also to be understood that the point of attachment of a multiple condensed ring system (as defined above for a heterocycle) can be at any position of the multiple condensed ring system including a heterocycle, aryl and carbocycle portion of the ring. It is also to be understood that the point of attachment for a heterocycle or heterocycle multiple condensed ring system can be at any suitable atom of the heterocycle or heterocycle multiple condensed ring system including a carbon atom and a heteroatom (e.g., a nitrogen). In one embodiment the term heterocycle includes a C2-20 heterocycle. In one embodiment the term heterocycle includes a C2-7 heterocycle. In one embodiment the term heterocycle includes a C2-5 heterocycle. In one embodiment the term heterocycle includes a C2-4 heterocycle. Exemplary heterocycles include, but are not limited to aziridinyl, azetidinyl, pyrrolidinyl, piperidinyl, homopiperidinyl, morpholinyl, thiomorpholinyl, piperazinyl, tetrahydrofuranyl, dihydrooxazolyl, tetrahydropyranyl, tetrahydrothiopyranyl, 1,2,3,4-tetrahydroquinolyl, benzoxazinyl, dihydrooxazolyl, chromanyl, 1,2-dihydropyridinyl, 2,3-dihydrobenzofuranyl, 1,3-benzodioxolyl, 1,4-benzodioxanyl, spiro[cyclopropane-1,1′-isoindolinyl]-3′-one, isoindolinyl-1-one, 2-oxa-6-azaspiro[3.3]heptanyl, imidazolidin-2-one N-methylpiperidine, imidazolidine, pyrazolidine, butyrolactam, valerolactam, imidazolidinone, hydantoin, dioxolane, phthalimide, 1,4-dioxane, thiomorpholine, thiomorpholine-S-oxide, thiomorpholine-S,S-oxide, pyran, 3-pyrroline, thiopyran, pyrone, tetrhydrothiophene, quinuclidine, tropane, 2-azaspiro[3.3]heptane, (1R,5S)-3-azabicyclo[3.2.1]octane, (1 s,4s)-2-azabicyclo[2.2.2]octane, (1R,4R)-2-oxa-5-azabicyclo[2.2.2]octane and pyrrolidin-2-one. In one embodiment the term “heterocycle” refers to a monocyclic, saturated or partially unsaturated, 3-8 membered ring having at least one heteroatom. For example, the term includes a monocyclic, saturated or partially unsaturated, 4, 5, 6, or 7 membered ring having at least one heteroatom. Non-limiting examples of heterocycle include aziridine, azetidine, pyrrolidine, piperidine, piperidine, piperazine, oxirane, morpholine, and thiomorpholine. The term “9- or 10-membered heterobicycle” as used herein refers to a partially unsaturated or aromatic fused bicyclic ring system having at least one heteroatom. For example, the term 9- or 10-membered heterobicycle includes a bicyclic ring system having a benzo ring fused to a 5-membered or 6-membered saturated, partially unsaturated, or aromatic ring that contains one or more heteroatoms.
  • As used herein, the term “heteroatom” is meant to include oxygen (O), nitrogen (N), sulfur (S) and silicon (Si). The nitrogen and sulfur can be in an oxidized form when feasible.
  • As used herein, the term “chiral” refers to molecules which have the property of non-superimposability of the mirror image partner, while the term “achiral” refers to molecules which are superimposable on their mirror image partner.
  • As used herein, the term “stereoisomers” refers to compounds which have identical chemical constitution, but differ with regard to the arrangement of the atoms or groups in space.
  • As used herein a wavy line “
    Figure US20200115354A1-20200416-P00001
    ” that intersects a bond in a chemical structure indicates the point of attachment of the bond that the wavy bond intersects in the chemical structure to the remainder of a molecule.
  • “Diastereomer” refers to a stereoisomer with two or more centers of chirality and whose molecules are not mirror images of one another. Diastereomers have different physical properties, e.g. melting points, boiling points, spectral properties, and reactivities. Mixtures of diastereomers can separate under high resolution analytical procedures such as electrophoresis and chromatography.
  • “Enantiomers” refer to two stereoisomers of a compound which are non-superimposable mirror images of one another.
  • Stereochemical definitions and conventions used herein generally follow S. P. Parker, Ed., McGraw-Hill Dictionary of Chemical Terms (1984) McGraw-Hill Book Company, New York; and Eliel, E. and Wilen, S., “Stereochemistry of Organic Compounds”, John Wiley & Sons, Inc., New York, 1994. The compounds of the invention can contain asymmetric or chiral centers, and therefore exist in different stereoisomeric forms. It is intended that all stereoisomeric forms of the compounds of the invention, including but not limited to, diastereomers, enantiomers and atropisomers, as well as mixtures thereof such as racemic mixtures, form part of the present invention. Many organic compounds exist in optically active forms, i.e., they have the ability to rotate the plane of plane-polarized light. In describing an optically active compound, the prefixes D and L, or R and S, are used to denote the absolute configuration of the molecule about its chiral center(s). The prefixes d and l or (+) and (−) are employed to designate the sign of rotation of plane-polarized light by the compound, with (−) or l meaning that the compound is levorotatory. A compound prefixed with (+) or d is dextrorotatory. For a given chemical structure, these stereoisomers are identical except that they are mirror images of one another. A specific stereoisomer can also be referred to as an enantiomer, and a mixture of such isomers is often called an enantiomeric mixture.
  • A 50:50 mixture of enantiomers is referred to as a racemic mixture or a racemate, which can occur where there has been no stereoselection or stereospecificity in a chemical reaction or process. The terms “racemic mixture” and “racemate” refer to an equimolar mixture of two enantiomeric species, devoid of optical activity.
  • When a bond in a compound formula herein is drawn in a non-stereochemical manner (e.g. flat), the atom to which the bond is attached includes all stereochemical possibilities. When a bond in a compound formula herein is drawn in a defined stereochemical manner (e.g. bold, bold-wedge, dashed or dashed-wedge), it is to be understood that the atom to which the stereochemical bond is attached is enriched in the absolute stereoisomer depicted unless otherwise noted. In one embodiment, the compound may be at least 51% the absolute stereoisomer depicted. In another embodiment, the compound may be at least 80% the absolute stereoisomer depicted. In another embodiment, the compound may be at least 90% the absolute stereoisomer depicted. In another embodiment, the compound may be at least 95% the absolute stereoisomer depicted. In another embodiment, the compound may be at least 97% the absolute stereoisomer depicted. In another embodiment, the compound may be at least 98% the absolute stereoisomer depicted. In another embodiment, the compound may be at least 99% the absolute stereoisomer depicted.
  • As used herein, the term “tautomer” or “tautomeric form” refers to structural isomers of different energies which are interconvertible via a low energy barrier. For example, proton tautomers (also known as prototropic tautomers) include interconversions via migration of a proton, such as keto-enol and imine-enamine isomerizations. Valence tautomers include interconversions by reorganization of some of the bonding electrons.
  • As used herein, the term “solvate” refers to an association or complex of one or more solvent molecules and a compound of the invention. Examples of solvents that form solvates include, but are not limited to, water, isopropanol, ethanol, methanol, DMSO, ethyl acetate, acetic acid, and ethanolamine. The term “hydrate” refers to the complex where the solvent molecule is water.
  • As used herein, the term “protecting group” refers to a substituent that is commonly employed to block or protect a particular functional group on a compound. For example, an “amino-protecting group” is a substituent attached to an amino group that blocks or protects the amino functionality in the compound. Suitable amino-protecting groups include acetyl, trifluoroacetyl, t-butoxycarbonyl (BOC), benzyloxycarbonyl (CBZ) and 9-fluorenylmethylenoxycarbonyl (Fmoc). Similarly, a “hydroxy-protecting group” refers to a substituent of a hydroxy group that blocks or protects the hydroxy functionality. Suitable protecting groups include acetyl and silyl. A “carboxy-protecting group” refers to a substituent of the carboxy group that blocks or protects the carboxy functionality. Common carboxy-protecting groups include phenylsulfonylethyl, cyanoethyl, 2-(trimethylsilyl)ethyl, 2-(trimethylsilyl)ethoxymethyl, 2-(p-toluenesulfonyl)ethyl, 2-(p-nitrophenylsulfenyl)ethyl, 2-(diphenylphosphino)-ethyl, nitroethyl and the like. For a general description of protecting groups and their use, see P. G. M. Wuts and T. W. Greene, Greene's Protective Groups in Organic Synthesis 4th edition, Wiley-Interscience, New York, 2006.
  • As used herein, the term “mammal” includes, but is not limited to, humans, mice, rats, guinea pigs, monkeys, dogs, cats, horses, cows, pigs, and sheep.
  • As used herein, the term “pharmaceutically acceptable salts” is meant to include salts of the active compounds which are prepared with relatively nontoxic acids or bases, depending on the particular substituents found on the compounds described herein. When compounds of the present invention contain relatively acidic functionalities, base addition salts can be obtained by contacting the neutral form of such compounds with a sufficient amount of the desired base, either neat or in a suitable inert solvent. Examples of salts derived from pharmaceutically-acceptable inorganic bases include aluminum, ammonium, calcium, copper, ferric, ferrous, lithium, magnesium, manganic, manganous, potassium, sodium, zinc and the like. Salts derived from pharmaceutically-acceptable organic bases include salts of primary, secondary and tertiary amines, including substituted amines, cyclic amines, naturally-occurring amines and the like, such as arginine, betaine, caffeine, choline, N,N′-dibenzylethylenediamine, diethylamine, 2-diethylaminoethanol, 2-dimethylaminoethanol, ethanolamine, ethylenediamine, N-ethylmorpholine, N-ethylpiperidine, glucamine, glucosamine, histidine, hydrabamine, isopropylamine, lysine, methylglucamine, morpholine, piperazine, piperidine, polyamine resins, procaine, purines, theobromine, triethylamine, trimethylamine, tripropylamine, tromethamine and the like. When compounds of the present invention contain relatively basic functionalities, acid addition salts can be obtained by contacting the neutral form of such compounds with a sufficient amount of the desired acid, either neat or in a suitable inert solvent. Examples of pharmaceutically acceptable acid addition salts include those derived from inorganic acids like hydrochloric, hydrobromic, nitric, carbonic, monohydrogencarbonic, phosphoric, monohydrogenphosphoric, dihydrogenphosphoric, sulfuric, monohydrogensulfuric, hydriodic, or phosphorous acids and the like, as well as the salts derived from relatively nontoxic organic acids like acetic, propionic, isobutyric, malonic, benzoic, succinic, suberic, fumaric, mandelic, phthalic, benzenesulfonic, p-tolylsulfonic, citric, tartaric, methanesulfonic, and the like. Also included are salts of amino acids such as arginate and the like, and salts of organic acids like glucuronic or galactunoric acids and the like (see, for example, Berge, S. M., et al., “Pharmaceutical Salts”, Journal of Pharmaceutical Science, 1977, 66, 1-19). Certain specific compounds of the present invention contain both basic and acidic functionalities that allow the compounds to be converted into either base or acid addition salts.
  • The neutral forms of the compounds can be regenerated by contacting the salt with a base or acid and isolating the parent compound in the conventional manner. The parent form of the compound differs from the various salt forms in certain physical properties, such as solubility in polar solvents, but otherwise the salts are equivalent to the parent form of the compound for the purposes of the present invention.
  • In one embodiment, W− is a pharmaceutically acceptable anion. In another embodiment W− is chloride, bromide, iodide, tosylate, methanesulfonate, acetate, citrate, malonate, tartrate, succinate, fumarate, benzoate, ascorbate, α-ketoglutarate, α-glycerophosphate, benzenesulfonate, lactate, bicarbonate, malate, maleate, carbonate, methylbromide, chloride, nitrate, gluconate, salicylate, glutamate, stearate, or sulfate.
  • In addition to salt forms, the present invention provides compounds which are in a prodrug form. As used herein the term “prodrug” refers to those compounds that readily undergo chemical changes under physiological conditions to provide the compounds of the present invention. Additionally, prodrugs can be converted to the compounds of the present invention by chemical or biochemical methods in an ex vivo environment. For example, prodrugs can be slowly converted to the compounds of the present invention when placed in a transdermal patch reservoir with a suitable enzyme or chemical reagent.
  • Prodrugs of the invention include compounds wherein an amino acid residue, or a polypeptide chain of two or more (e.g., two, three or four) amino acid residues, is covalently joined through an amide or ester bond to a free amino, hydroxy or carboxylic acid group of a compound of the present invention. The amino acid residues include but are not limited to the 20 naturally occurring amino acids commonly designated by three letter symbols and also includes phosphoserine, phosphothreonine, phosphotyrosine, 4-hydroxyproline, hydroxylysine, demosine, isodemosine, gamma-carboxyglutamate, hippuric acid, octahydroindole-2-carboxylic acid, statine, 1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid, penicillamine, ornithine, 3-methylhistidine, norvaline, beta-alanine, gamma-aminobutyric acid, citrulline, homocysteine, homoserine, methyl-alanine, para-benzoylphenylalanine, phenylglycine, propargylglycine, sarcosine, methionine sulfone and tert-butylglycine.
  • Additional types of prodrugs are also encompassed. For instance, a free carboxyl group of a compound of the invention can be derivatized as an amide or alkyl ester. As another example, compounds of this invention comprising free hydroxy groups can be derivatized as prodrugs by converting the hydroxy group into a group such as, but not limited to, a phosphate ester, hemisuccinate, dimethylaminoacetate, or phosphoryloxymethyloxycarbonyl group, as outlined in Fleisher, D. et al., (1996) Improved oral drug delivery: solubility limitations overcome by the use of prodrugs Advanced Drug Delivery Reviews, 19:115. Carbamate prodrugs of hydroxy and amino groups are also included, as are carbonate prodrugs, sulfonate esters and sulfate esters of hydroxy groups. Derivatization of hydroxy groups as (acyloxy)methyl and (acyloxy)ethyl ethers, wherein the acyl group can be an alkyl ester optionally substituted with groups including, but not limited to, ether, amine and carboxylic acid functionalities, or where the acyl group is an amino acid ester as described above, are also encompassed. Prodrugs of this type are described in J. Med. Chem., (1996), 39:10. More specific examples include replacement of the hydrogen atom of the alcohol group with a group such as (C1-6)alkanoyloxymethyl, 1-((C1-6)alkanoyloxy)ethyl, 1-methyl-1-((C1-6)alkanoyloxy)ethyl, (C1-6)alkoxycarbonyloxymethyl, N—(C1-6)alkoxycarbonylaminomethyl, succinoyl, (C1-6)alkanoyl, alpha-amino(C1-4)alkanoyl, arylacyl and alpha-aminoacyl, or alpha-aminoacyl-alpha-aminoacyl, where each alpha-aminoacyl group is independently selected from the naturally occurring L-amino acids, P(O)(OH)2, —P(O)(O(C1-6)alkyl)2 or glycosyl (the radical resulting from the removal of a hydroxyl group of the hemiacetal form of a carbohydrate).
  • For additional examples of prodrug derivatives, see, for example, a) Design of Prodrugs, edited by H. Bundgaard, (Elsevier, 1985) and Methods in Enzymology, Vol. 42, p. 309-396, edited by K. Widder, et al. (Academic Press, 1985); b) A Textbook of Drug Design and Development, edited by Krogsgaard-Larsen and H. Bundgaard, Chapter 5 “Design and Application of Prodrugs,” by H. Bundgaard p. 113-191 (1991); c) H. Bundgaard, Advanced Drug Delivery Reviews, 8:1-38 (1992); d) H. Bundgaard, et al., Journal of Pharmaceutical Sciences, 77:285 (1988); and e) N. Kakeya, et al., Chem. Pharm. Bull., 32:692 (1984), each of which is specifically incorporated herein by reference.
  • Additionally, the present invention provides for metabolites of compounds of the invention. As used herein, a “metabolite” refers to a product produced through metabolism in the body of a specified compound or salt thereof. Such products can result for example from the oxidation, reduction, hydrolysis, amidation, deamidation, esterification, deesterification, enzymatic cleavage, and the like, of the administered compound.
  • Metabolite products typically are identified by preparing a radiolabelled (e.g., 14C or 3H) isotope of a compound of the invention, administering it parenterally in a detectable dose (e.g., greater than about 0.5 mg/kg) to an animal such as rat, mouse, guinea pig, monkey, or to man, allowing sufficient time for metabolism to occur (typically about 30 seconds to 30 hours) and isolating its conversion products from the urine, blood or other biological samples. These products are easily isolated since they are labeled (others are isolated by the use of antibodies capable of binding epitopes surviving in the metabolite). The metabolite structures are determined in conventional fashion, e.g., by MS, LC/MS or NMR analysis. In general, analysis of metabolites is done in the same way as conventional drug metabolism studies well known to those skilled in the art. The metabolite products, so long as they are not otherwise found in vivo, are useful in diagnostic assays for therapeutic dosing of the compounds of the invention.
    • Compounds
  • In one aspect the present invention provides for compounds of Formula I and its embodiments as described hereinbove.
  • In another embodiment, the compound is selected from compounds of formula I as described in the Examples herein and salts thereof.
    • Synthesis of Compounds
  • Compounds of formula (I) may be prepared as illustrated in Scheme 1 below and as shown in the Examples herein.
  • Figure US20200115354A1-20200416-C00104
    • Pharmaceutical Compositions and Administration
  • In addition to one or more of the compounds provided above (or stereoisomers, geometric isomers, tautomers, solvates, metabolites, isotopes, pharmaceutically acceptable salts, or prodrugs thereof), the invention also provides for compositions and medicaments comprising a compound of formula I or and embodiment thereof and at least one pharmaceutically acceptable carrier, diluent or excipient. The compositions of the invention can be used to selectively inhibit NaV1.7 in patients (e.g, humans).
  • The term “composition,” as used herein, is intended to encompass a product comprising the specified ingredients in the specified amounts, as well as any product which results, directly or indirectly, from combination of the specified ingredients in the specified amounts. By “pharmaceutically acceptable” it is meant the carrier, diluent or excipient must be compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.
  • In one embodiment, the invention provides for pharmaceutical compositions (or medicaments) comprising a compound of formula I or an embodiment thereof, and its stereoisomers, geometric isomers, tautomers, solvates, metabolites, isotopes, pharmaceutically acceptable salts, or prodrugs thereof) and a pharmaceutically acceptable carrier, diluent or excipient. In another embodiment, the invention provides for preparing compositions (or medicaments) comprising compounds of the invention. In another embodiment, the invention provides for administering compounds of formula I or its embodiments and compositions comprising compounds of formula I or an embodiment thereof to a patient (e.g., a human patient) in need thereof.
  • Compositions are formulated, dosed, and administered in a fashion consistent with good medical practice. Factors for consideration in this context include the particular disorder being treated, the particular mammal being treated, the clinical condition of the individual patient, the cause of the disorder, the site of delivery of the agent, the method of administration, the scheduling of administration, and other factors known to medical practitioners. The effective amount of the compound to be administered will be governed by such considerations, and is the minimum amount necessary to inhibit NaV1.7 activity as required to prevent or treat the undesired disease or disorder, such as for example, pain. For example, such amount may be below the amount that is toxic to normal cells, or the mammal as a whole.
  • In one example, the therapeutically effective amount of the compound of the invention administered parenterally per dose will be in the range of about 0.01-100 mg/kg, alternatively about e.g., 0.1 to 20 mg/kg of patient body weight per day, with the typical initial range of compound used being 0.3 to 15 mg/kg/day. The daily does is, in certain embodiments, given as a single daily dose or in divided doses two to six times a day, or in sustained release form. In the case of a 70 kg adult human, the total daily dose will generally be from about 7 mg to about 1,400 mg. This dosage regimen may be adjusted to provide the optimal therapeutic response. The compounds may be administered on a regimen of 1 to 4 times per day, preferably once or twice per day.
  • The compounds of the present invention may be administered in any convenient administrative form, e.g., tablets, powders, capsules, solutions, dispersions, suspensions, syrups, sprays, suppositories, gels, emulsions, patches, etc. Such compositions may contain components conventional in pharmaceutical preparations, e.g., diluents, carriers, pH modifiers, sweeteners, bulking agents, and further active agents.
  • The compounds of the invention may be administered by any suitable means, including oral, topical (including buccal and sublingual), rectal, vaginal, transdermal, parenteral, subcutaneous, intraperitoneal, intrapulmonary, intradermal, intrathecal and epidural and intranasal, and, if desired for local treatment, intralesional administration. Parenteral infusions include intramuscular, intravenous, intraarterial, intraperitoneal, intracerebral, intraocular, intralesional or subcutaneous administration.
  • The compositions comprising compounds of formula I or an embodiment thereof are normally formulated in accordance with standard pharmaceutical practice as a pharmaceutical composition. A typical formulation is prepared by mixing a compound of the present invention and a diluent, carrier or excipient. Suitable diluents, carriers and excipients are well known to those skilled in the art and are described in detail in, e.g., Ansel, Howard C., et al., Ansel's Pharmaceutical Dosage Forms and Drug Delivery Systems. Philadelphia: Lippincott, Williams & Wilkins, 2004; Gennaro, Alfonso R., et al. Remington: The Science and Practice of Pharmacy. Philadelphia: Lippincott, Williams & Wilkins, 2000; and Rowe, Raymond C. Handbook of Pharmaceutical Excipients. Chicago, Pharmaceutical Press, 2005. The formulations may also include one or more buffers, stabilizing agents, surfactants, wetting agents, lubricating agents, emulsifiers, suspending agents, preservatives, antioxidants, opaquing agents, glidants, processing aids, colorants, sweeteners, perfuming agents, flavoring agents, diluents and other known additives to provide an elegant presentation of the drug (i.e., a compound of the present invention or pharmaceutical composition thereof) or aid in the manufacturing of the pharmaceutical product (i.e., medicament).
  • Suitable carriers, diluents and excipients are well known to those skilled in the art and include materials such as carbohydrates, waxes, water soluble and/or swellable polymers, hydrophilic or hydrophobic materials, gelatin, oils, solvents, water and the like. The particular carrier, diluent or excipient used will depend upon the means and purpose for which a compound of the present invention is being applied. Solvents are generally selected based on solvents recognized by persons skilled in the art as safe (GRAS) to be administered to a mammal. In general, safe solvents are non-toxic aqueous solvents such as water and other non-toxic solvents that are soluble or miscible in water. Suitable aqueous solvents include water, ethanol, propylene glycol, polyethylene glycols (e.g., PEG 400, PEG 300), etc. and mixtures thereof. The formulations can also include one or more buffers, stabilizing agents, surfactants, wetting agents, lubricating agents, emulsifiers, suspending agents, preservatives, antioxidants, opaquing agents, glidants, processing aids, colorants, sweeteners, perfuming agents, flavoring agents and other known additives to provide an elegant presentation of the drug (i.e., a compound of the present invention or pharmaceutical composition thereof) or aid in the manufacturing of the pharmaceutical product (i.e., medicament).
  • Acceptable diluents, carriers, excipients and stabilizers are nontoxic to recipients at the dosages and concentrations employed, and include buffers such as phosphate, citrate and other organic acids; antioxidants including ascorbic acid and methionine; preservatives (such as octadecyldimethylbenzyl ammonium chloride; hexamethonium chloride; benzalkonium chloride, benzethonium chloride; phenol, butyl or benzyl alcohol; alkyl parabens such as methyl or propyl paraben; catechol; resorcinol; cyclohexanol; 3-pentanol; and m-cresol); low molecular weight (less than about 10 residues) polypeptides; proteins, such as serum albumin, gelatin, or immunoglobulins; hydrophilic polymers such as polyvinylpyrrolidone; amino acids such as glycine, glutamine, asparagine, histidine, arginine, or lysine; monosaccharides, disaccharides and other carbohydrates including glucose, mannose, or dextrins; chelating agents such as EDTA; sugars such as sucrose, mannitol, trehalose or sorbitol; salt-forming counter-ions such as sodium; metal complexes (e.g., Zn-protein complexes); and/or non-ionic surfactants such as TWEEN™, PLURONICS™ or polyethylene glycol (PEG). A active pharmaceutical ingredient of the invention (e.g., compound of formula I or an embodiment thereof) can also be entrapped in microcapsules prepared, for example, by coacervation techniques or by interfacial polymerization, for example, hydroxymethylcellulose or gelatin-microcapsules and poly-(methylmethacylate) microcapsules, respectively, in colloidal drug delivery systems (for example, liposomes, albumin microspheres, microemulsions, nano-particles and nanocapsules) or in macroemulsions. Such techniques are disclosed in Remington: The Science and Practice of Pharmacy: Remington the Science and Practice of Pharmacy (2005) 21st Edition, Lippincott Williams & Wilkins, Philidelphia, Pa.
  • Sustained-release preparations of a compound of the invention (e.g., compound of formula I or an embodiment thereof) can be prepared. Suitable examples of sustained-release preparations include semipermeable matrices of solid hydrophobic polymers containing a compound of formula I or an embodiment thereof, which matrices are in the form of shaped articles, e.g., films, or microcapsules. Examples of sustained-release matrices include polyesters, hydrogels (for example, poly(2-hydroxyethyl-methacrylate), or poly(vinyl alcohol)), polylactides (U.S. Pat. No. 3,773,919), copolymers of L-glutamic acid and gamma-ethyl-L-glutamate (Sidman et al., Biopolymers 22:547, 1983), non-degradable ethylene-vinyl acetate (Langer et al., J. Biomed. Mater. Res. 15:167, 1981), degradable lactic acid-glycolic acid copolymers such as the LUPRON DEPOT™ (injectable microspheres composed of lactic acid-glycolic acid copolymer and leuprolide acetate) and poly-D-(−)-3-hydroxybutyric acid (EP 133,988A). Sustained release compositions also include liposomally entrapped compounds, which can be prepared by methods known per se (Epstein et al., Proc. Natl. Acad. Sci. U.S.A. 82:3688, 1985; Hwang et al., Proc. Natl. Acad. Sci. U.S.A. 77:4030, 1980; U.S. Pat. Nos. 4,485,045 and 4,544,545; and EP 102,324A). Ordinarily, the liposomes are of the small (about 200-800 Angstroms) unilamelar type in which the lipid content is greater than about 30 mol % cholesterol, the selected proportion being adjusted for the optimal therapy.
  • The formulations include those suitable for the administration routes detailed herein. The formulations can conveniently be presented in unit dosage form and can be prepared by any of the methods well known in the art of pharmacy. Techniques and formulations generally are found in Remington: The Science and Practice of Pharmacy: Remington the Science and Practice of Pharmacy (2005) 21st Edition, Lippincott Williams & Wilkins, Philidelphia, Pa. Such methods include the step of bringing into association the active ingredient with the carrier which constitutes one or more accessory ingredients.
  • In general the formulations are prepared by uniformly and intimately bringing into association the active ingredient with liquid carriers, diluents or excipients or finely divided solid carriers, diluents or excipients, or both, and then, if necessary, shaping the product. A typical formulation is prepared by mixing a compound of the present invention and a carrier, diluent or excipient. The formulations can be prepared using conventional dissolution and mixing procedures. For example, the bulk drug substance (i.e., compound of the present invention or stabilized form of the compound (e.g., complex with a cyclodextrin derivative or other known complexation agent) is dissolved in a suitable solvent in the presence of one or more of the excipients described above. A compound of the present invention is typically formulated into pharmaceutical dosage forms to provide an easily controllable dosage of the drug and to enable patient compliance with the prescribed regimen.
  • In one example, compounds of formula I or an embodiment thereof may be formulated by mixing at ambient temperature at the appropriate pH, and at the desired degree of purity, with physiologically acceptable carriers, i.e., carriers that are non-toxic to recipients at the dosages and concentrations employed into a galenical administration form. The pH of the formulation depends mainly on the particular use and the concentration of compound, but preferably ranges anywhere from about 3 to about 8. In one example, a compound of formula I (or an embodiment thereof) is formulated in an acetate buffer, at pH 5. In another embodiment, the compounds of formula I or an embodiment thereof are sterile. The compound may be stored, for example, as a solid or amorphous composition, as a lyophilized formulation or as an aqueous solution.
  • Formulations of a compound of the invention (e.g., compound of formula I or an embodiment thereof) suitable for oral administration can be prepared as discrete units such as pills, capsules, cachets or tablets each containing a predetermined amount of a compound of the invention.
  • Compressed tablets can be prepared by compressing in a suitable machine the active ingredient in a free-flowing form such as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, preservative, surface active or dispersing agent. Molded tablets can be made by molding in a suitable machine a mixture of the powdered active ingredient moistened with an inert liquid diluent. The tablets can optionally be coated or scored and optionally are formulated so as to provide slow or controlled release of the active ingredient therefrom.
  • Tablets, troches, lozenges, aqueous or oil suspensions, dispersible powders or granules, emulsions, hard or soft capsules, e.g., gelatin capsules, syrups or elixirs can be prepared for oral use. Formulations of a compound of the invention (e.g., compound of formula I or an embodiment thereof) intended for oral use can be prepared according to any method known to the art for the manufacture of pharmaceutical compositions and such compositions can contain one or more agents including sweetening agents, flavoring agents, coloring agents and preserving agents, in order to provide a palatable preparation. Tablets containing the active ingredient in admixture with non-toxic pharmaceutically acceptable excipient which are suitable for manufacture of tablets are acceptable. These excipients can be, for example, inert diluents, such as calcium or sodium carbonate, lactose, calcium or sodium phosphate; granulating and disintegrating agents, such as maize starch, or alginic acid; binding agents, such as starch, gelatin or acacia; and lubricating agents, such as magnesium stearate, stearic acid or talc. Tablets can be uncoated or can be coated by known techniques including microencapsulation to delay disintegration and adsorption in the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl monostearate or glyceryl distearate alone or with a wax can be employed.
  • An example of a suitable oral administration form is a tablet containing about 1 mg, 5 mg, 10 mg, 25 mg, 30 mg, 50 mg, 80 mg, 100 mg, 150 mg, 250 mg, 300 mg and 500 mg of the compound of the invention compounded with about 90-30 mg anhydrous lactose, about 5-40 mg sodium croscarmellose, about 5-30 mg polyvinylpyrrolidone (PVP) K30, and about 1-10 mg magnesium stearate. The powdered ingredients are first mixed together and then mixed with a solution of the PVP. The resulting composition can be dried, granulated, mixed with the magnesium stearate and compressed to tablet form using conventional equipment. An example of an aerosol formulation can be prepared by dissolving the compound, for example 5-400 mg, of the invention in a suitable buffer solution, e.g. a phosphate buffer, adding a tonicifier, e.g. a salt such sodium chloride, if desired. The solution may be filtered, e.g., using a 0.2 micron filter, to remove impurities and contaminants.
  • For treatment of the eye or other external tissues, e.g., mouth and skin, the formulations are preferably applied as a topical ointment or cream containing the active ingredient(s) in an amount of, for example, 0.075 to 20% w/w. When formulated in an ointment, the active ingredient can be employed with either a paraffinic or a water-miscible ointment base. Alternatively, the active ingredients can be formulated in a cream with an oil-in-water cream base. If desired, the aqueous phase of the cream base can include a polyhydric alcohol, i.e., an alcohol having two or more hydroxyl groups such as propylene glycol, butane 1,3-diol, mannitol, sorbitol, glycerol and polyethylene glycol (including PEG 400) and mixtures thereof. The topical formulations can desirably include a compound which enhances absorption or penetration of the active ingredient through the skin or other affected areas. Examples of such dermal penetration enhancers include dimethyl sulfoxide and related analogs.
  • The oily phase of the emulsions of this invention can be constituted from known ingredients in a known manner. While the phase can comprise merely an emulsifier, it desirably comprises a mixture of at least one emulsifier with a fat or an oil or with both a fat and an oil. Preferably, a hydrophilic emulsifier is included together with a lipophilic emulsifier which acts as a stabilizer. It is also preferred to include both an oil and a fat. Together, the emulsifier(s) with or without stabilizer(s) make up the so-called emulsifying wax, and the wax together with the oil and fat make up the so-called emulsifying ointment base which forms the oily dispersed phase of the cream formulations. Emulsifiers and emulsion stabilizers suitable for use in the formulation of the invention include Tween® 60, Span® 80, cetostearyl alcohol, benzyl alcohol, myristyl alcohol, glyceryl mono-stearate and sodium lauryl sulfate.
  • In one aspect of topical applications, it is desired to administer an effective amount of a pharmaceutical composition according to the invention to target area, e.g., skin surfaces, mucous membranes, and the like, which are adjacent to peripheral neurons which are to be treated. This amount will generally range from about 0.0001 mg to about 1 g of a compound of the invention per application, depending upon the area to be treated, whether the use is diagnostic, prophylactic or therapeutic, the severity of the symptoms, and the nature of the topical vehicle employed. A preferred topical preparation is an ointment, wherein about 0.001 to about 50 mg of active ingredient is used per cc of ointment base. The pharmaceutical composition can be formulated as transdermal compositions or transdermal delivery devices (“patches”). Such compositions include, for example, a backing, active compound reservoir, a control membrane, liner and contact adhesive. Such transdermal patches may be used to provide continuous pulsatile, or on demand delivery of the compounds of the present invention as desired.
  • Aqueous suspensions of a compound of the invention (e.g., compound of formula I or an embodiment thereof) contain the active materials in admixture with excipients suitable for the manufacture of aqueous suspensions. Such excipients include a suspending agent, such as sodium carboxymethylcellulose, croscarmellose, povidone, methylcellulose, hydroxypropyl methylcellulose, sodium alginate, polyvinylpyrrolidone, gum tragacanth and gum acacia, and dispersing or wetting agents such as a naturally occurring phosphatide (e.g., lecithin), a condensation product of an alkylene oxide with a fatty acid (e.g., polyoxyethylene stearate), a condensation product of ethylene oxide with a long chain aliphatic alcohol (e.g., heptadecaethyleneoxycetanol), a condensation product of ethylene oxide with a partial ester derived from a fatty acid and a hexitol anhydride (e.g., polyoxyethylene sorbitan monooleate). The aqueous suspension can also contain one or more preservatives such as ethyl or n-propyl p-hydroxybenzoate, one or more coloring agents, one or more flavoring agents and one or more sweetening agents, such as sucrose or saccharin.
  • Formulations of a compound of the invention (e.g., compound of formula I or an embodiment thereof) can be in the form of a sterile injectable preparation, such as a sterile injectable aqueous or oleaginous suspension. This suspension can be formulated according to the known art using those suitable dispersing or wetting agents and suspending agents which have been mentioned above. The sterile injectable preparation can also be a sterile injectable solution or suspension in a non-toxic parenterally acceptable diluent or solvent, such as a solution in 1,3-butanediol or prepared as a lyophilized powder.
  • Among the acceptable vehicles and solvents that can be employed are water, Ringer's solution and isotonic sodium chloride solution. In addition, sterile fixed oils can conventionally be employed as a solvent or suspending medium. For this purpose any bland fixed oil can be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid can likewise be used in the preparation of injectables.
  • The amount of active ingredient that can be combined with the carrier material to produce a single dosage form will vary depending upon the host treated and the particular mode of administration. For example, a time-release formulation intended for oral administration to humans can contain approximately 1 to 1000 mg of active material compounded with an appropriate and convenient amount of carrier material which can vary from about 5 to about 95% of the total compositions (weight:weight). The pharmaceutical composition can be prepared to provide easily measurable amounts for administration. For example, an aqueous solution intended for intravenous infusion can contain from about 3 to 500 μg of the active ingredient per milliliter of solution in order that infusion of a suitable volume at a rate of about 30 mL/hr can occur.
  • Formulations suitable for parenteral administration include aqueous and non-aqueous sterile injection solutions which can contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which can include suspending agents and thickening agents.
  • Formulations suitable for topical administration to the eye also include eye drops wherein the active ingredient is dissolved or suspended in a suitable carrier, especially an aqueous solvent for the active ingredient. The active ingredient is preferably present in such formulations in a concentration of about 0.5 to 20% w/w, for example about 0.5 to 10% w/w, for example about 1.5% w/w.
  • Formulations suitable for topical administration in the mouth include lozenges comprising the active ingredient in a flavored basis, usually sucrose and acacia or tragacanth; pastilles comprising the active ingredient in an inert basis such as gelatin and glycerin, or sucrose and acacia; and mouthwashes comprising the active ingredient in a suitable liquid carrier.
  • Formulations for rectal administration can be presented as a suppository with a suitable base comprising for example cocoa butter or a salicylate.
  • Formulations suitable for intrapulmonary or nasal administration have a particle size for example in the range of 0.1 to 500 microns (including particle sizes in a range between 0.1 and 500 microns in increments microns such as 0.5, 1, 30 microns, 35 microns, etc.), which is administered by rapid inhalation through the nasal passage or by inhalation through the mouth so as to reach the alveolar sacs. Suitable formulations include aqueous or oily solutions of the active ingredient. Formulations suitable for aerosol or dry powder administration can be prepared according to conventional methods and can be delivered with other therapeutic agents such as compounds heretofore used in the treatment of disorders as described below.
  • The formulations can be packaged in unit-dose or multi-dose containers, for example sealed ampoules and vials, and can be stored in a freeze-dried (lyophilized) condition requiring only the addition of the sterile liquid carrier, for example water, for injection immediately prior to use. Extemporaneous injection solutions and suspensions are prepared from sterile powders, granules and tablets of the kind previously described. Preferred unit dosage formulations are those containing a daily dose or unit daily sub-dose, as herein above recited, or an appropriate fraction thereof, of the active ingredient.
  • When the binding target is located in the brain, certain embodiments of the invention provide for a compound of formula I (or an embodiment thereof) to traverse the blood-brain barrier. Certain neurodegenerative diseases are associated with an increase in permeability of the blood-brain barrier, such that a compound of formula I (or an embodiment thereof) can be readily introduced to the brain. When the blood-brain barrier remains intact, several art-known approaches exist for transporting molecules across it, including, but not limited to, physical methods, lipid-based methods, and receptor and channel-based methods.
  • Physical methods of transporting a compound of formula I (or an embodiment thereof) across the blood-brain barrier include, but are not limited to, circumventing the blood-brain barrier entirely, or by creating openings in the blood-brain barrier.
  • Circumvention methods include, but are not limited to, direct injection into the brain (see, e.g., Papanastassiou et al., Gene Therapy 9:398-406, 2002), interstitial infusion/convection-enhanced delivery (see, e.g., Bobo et al., Proc. Natl. Acad. Sci. U.S.A. 91:2076-2080, 1994), and implanting a delivery device in the brain (see, e.g., Gill et al., Nature Med. 9:589-595, 2003; and Gliadel Wafers™, Guildford Pharmaceutical). Methods of creating openings in the barrier include, but are not limited to, ultrasound (see, e.g., U.S. Patent Publication No. 2002/0038086), osmotic pressure (e.g., by administration of hypertonic mannitol (Neuwelt, E. A., Implication of the Blood-Brain Barrier and its Manipulation, Volumes 1 and 2, Plenum Press, N.Y., 1989)), and permeabilization by, e.g., bradykinin or permeabilizer A-7 (see, e.g., U.S. Pat. Nos. 5,112,596, 5,268,164, 5,506,206, and 5,686,416).
  • Lipid-based methods of transporting a compound of formula I (or an embodiment thereof) across the blood-brain barrier include, but are not limited to, encapsulating the a compound of formula I (or an embodiment thereof) in liposomes that are coupled to antibody binding fragments that bind to receptors on the vascular endothelium of the blood-brain barrier (see, e.g., U.S. Patent Application Publication No. 2002/0025313), and coating a compound of formula I (or an embodiment thereof) in low-density lipoprotein particles (see, e.g., U.S. Patent Application Publication No. 2004/0204354) or apolipoprotein E (see, e.g., U.S. Patent Application Publication No. 2004/0131692).
  • Receptor and channel-based methods of transporting a compound of formula I (or an embodiment thereof) across the blood-brain barrier include, but are not limited to, using glucocorticoid blockers to increase permeability of the blood-brain barrier (see, e.g., U.S. Patent Application Publication Nos. 2002/0065259, 2003/0162695, and 2005/0124533); activating potassium channels (see, e.g., U.S. Patent Application Publication No. 2005/0089473), inhibiting ABC drug transporters (see, e.g., U.S. Patent Application Publication No. 2003/0073713); coating a compound of formula I (or an embodiment thereof) with a transferrin and modulating activity of the one or more transferrin receptors (see, e.g., U.S. Patent Application Publication No. 2003/0129186), and cationizing the antibodies (see, e.g., U.S. Pat. No. 5,004,697).
  • For intracerebral use, in certain embodiments, the compounds can be administered continuously by infusion into the fluid reservoirs of the CNS, although bolus injection may be acceptable. The inhibitors can be administered into the ventricles of the brain or otherwise introduced into the CNS or spinal fluid. Administration can be performed by use of an indwelling catheter and a continuous administration means such as a pump, or it can be administered by implantation, e.g., intracerebral implantation of a sustained-release vehicle. More specifically, the inhibitors can be injected through chronically implanted cannulas or chronically infused with the help of osmotic minipumps. Subcutaneous pumps are available that deliver proteins through a small tubing to the cerebral ventricles. Highly sophisticated pumps can be refilled through the skin and their delivery rate can be set without surgical intervention. Examples of suitable administration protocols and delivery systems involving a subcutaneous pump device or continuous intracerebroventricular infusion through a totally implanted drug delivery system are those used for the administration of dopamine, dopamine agonists, and cholinergic agonists to Alzheimer's disease patients and animal models for Parkinson's disease, as described by Harbaugh, J. Neural Transm. Suppl. 24:271, 1987; and DeYebenes et al., Mov. Disord. 2: 143, 1987.
  • A compound of formula I (or an embodiment thereof) used in the invention are formulated, dosed, and administered in a fashion consistent with good medical practice. Factors for consideration in this context include the particular disorder being treated, the particular mammal being treated, the clinical condition of the individual patient, the cause of the disorder, the site of delivery of the agent, the method of administration, the scheduling of administration, and other factors known to medical practitioners. A compound of formula I (or an embodiment thereof) need not be, but is optionally formulated with one or more agent currently used to prevent or treat the disorder in question. The effective amount of such other agents depends on the amount of a compound of the invention present in the formulation, the type of disorder or treatment, and other factors discussed above.
  • These are generally used in the same dosages and with administration routes as described herein, or about from 1 to 99% of the dosages described herein, or in any dosage and by any route that is empirically/clinically determined to be appropriate.
  • For the prevention or treatment of disease, the appropriate dosage of a compound of formula I (or an embodiment thereof) (when used alone or in combination with other agents) will depend on the type of disease to be treated, the properties of the compound, the severity and course of the disease, whether the compound is administered for preventive or therapeutic purposes, previous therapy, the patient's clinical history and response to the compound, and the discretion of the attending physician. The compound is suitably administered to the patient at one time or over a series of treatments. Depending on the type and severity of the disease, about 1 μg/kg to 15 mg/kg (e.g., 0.1 mg/kg-10 mg/kg) of compound can be an initial candidate dosage for administration to the patient, whether, for example, by one or more separate administrations, or by continuous infusion. One typical daily dosage might range from about 1 μg kg to 100 mg/kg or more, depending on the factors mentioned above. For repeated administrations over several days or longer, depending on the condition, the treatment would generally be sustained until a desired suppression of disease symptoms occurs. One exemplary dosage of a compound of formula I (or an embodiment thereof) would be in the range from about 0.05 mg/kg to about 10 mg/kg. Thus, one or more doses of about 0.5 mg/kg, 2.0 mg/kg, 4.0 mg/kg, or 10 mg/kg (or any combination thereof) may be administered to the patient. Such doses may be administered intermittently, e.g., every week or every three weeks (e.g., such that the patient receives from about two to about twenty, or, e.g., about six doses of the antibody). An initial higher loading dose, followed by one or more lower doses may be administered. An exemplary dosing regimen comprises administering an initial loading dose of about 4 mg/kg, followed by a weekly maintenance dose of about 2 mg kg of the compound. However, other dosage regimens may be useful. The progress of this therapy is easily monitored by conventional techniques and assays.
  • Other typical daily dosages might range from, for example, about 1 g/kg to up to 100 mg/kg or more (e.g., about 1 μg kg to 1 mg/kg, about 1 μg/kg to about 5 mg/kg, about 1 mg kg to 10 mg/kg, about 5 mg/kg to about 200 mg/kg, about 50 mg/kg to about 150 mg/mg, about 100 mg/kg to about 500 mg/kg, about 100 mg/kg to about 400 mg/kg, and about 200 mg/kg to about 400 mg/kg), depending on the factors mentioned above. Typically, the clinician will administer a compound until a dosage is reached that results in improvement in or, optimally, elimination of, one or more symptoms of the treated disease or condition. The progress of this therapy is easily monitored by conventional assays. One or more agent provided herein may be administered together or at different times (e.g., one agent is administered prior to the administration of a second agent). One or more agent may be administered to a subject using different techniques (e.g., one agent may be administered orally, while a second agent is administered via intramuscular injection or intranasally). One or more agent may be administered such that the one or more agent has a pharmacologic effect in a subject at the same time. Alternatively, one or more agent may be administered, such that the pharmacological activity of the first administered agent is expired prior the administration of one or more secondarily administered agents (e.g., 1, 2, 3, or 4 secondarily administered agents).
    • Indications and Methods of Treatment
  • The compounds of the invention modulate, preferably inhibit, ion flux through a voltage-dependent sodium channel in a mammal, (e.g, a human). Any such modulation, whether it be partial or complete inhibition or prevention of ion flux, is sometimes referred to herein as “blocking” and corresponding compounds as “blockers” or “inhibitors”. In general, the compounds of the invention modulate the activity of a sodium channel downwards by inhibiting the voltage-dependent activity of the sodium channel, and/or reduce or prevent sodium ion flux across a cell membrane by preventing sodium channel activity such as ion flux.
  • Accordingly, the compounds of the invention are sodium channel blockers and are therefore useful for treating diseases and conditions in mammals, for example humans, and other organisms, including all those diseases and conditions which are the result of aberrant voltage-dependent sodium channel biological activity or which may be ameliorated by modulation of voltage-dependent sodium channel biological activity. In particular, the compounds of the invention, i.e., the compounds of formula (I) and embodiments and (or stereoisomers, geometric isomers, tautomers, solvates, metabolites, isotopes, pharmaceutically acceptable salts, or prodrugs thereof), are useful for treating diseases and conditions in mammals, for example humans, which are the result of aberrant voltage-dependent NaV1.7 biological activity or which may be ameliorated by the modulation, preferably the inhibition, of NaV1.7 biological activity. In certain aspects, the compounds of the invention selectively inhibit NaV1.7 over NaV1.5.
  • As defined herein, a sodium channel-mediated disease or condition refers to a disease or condition in a mammal, preferably a human, which is ameliorated upon modulation of the sodium channel and includes, but is not limited to, pain, central nervous conditions such as epilepsy, anxiety, depression and bipolar disease; cardiovascular conditions such as arrhythmias, atrial fibrillation and ventricular fibrillation; neuromuscular conditions such as restless leg syndrome and muscle paralysis or tetanus; neuroprotection against stroke, neural trauma and multiple sclerosis; and channelopathies such as erythromyalgia and familial rectal pain syndrome.
  • In one aspect, the present invention relates to compounds, pharmaceutical compositions and methods of using the compounds and pharmaceutical compositions for the treatment of sodium channel-mediated diseases in mammals, preferably humans and preferably diseases and conditions related to pain, central nervous conditions such as epilepsy, anxiety, depression and bipolar disease; cardiovascular conditions such as arrhythmias, atrial fibrillation and ventricular fibrillation; neuromuscular conditions such as restless leg syndrome and muscle paralysis or tetanus; neuroprotection against stroke, neural trauma and multiple sclerosis; and channelopathies such as erythromyalgia and familial rectal pain syndrome, by administering to a mammal, for example a human, in need of such treatment an effective amount of a sodium channel blocker modulating, especially inhibiting, agent.
  • A sodium channel-mediated disease or condition also includes pain associated with HIV, HIV treatment induced neuropathy, trigeminal neuralgia, glossopharyngeal neuralgia, neuropathy secondary to metastatic infiltration, adiposis dolorosa, thalamic lesions, hypertension, autoimmune disease, asthma, drug addiction (e.g., opiate, benzodiazepine, amphetamine, cocaine, alcohol, butane inhalation), Alzheimer, dementia, age-related memory impairment, Korsakoff syndrome, restenosis, urinary dysfunction, incontinence, Parkinson's disease, cerebrovascular ischemia, neurosis, gastrointestinal disease, sickle cell anemia, transplant rejection, heart failure, myocardial infarction, reperfusion injury, intermittant claudication, angina, convulsion, respiratory disorders, cerebral or myocardial ischemias, long-QT syndrome, Catecholeminergic polymorphic ventricular tachycardia, ophthalmic diseases, spasticity, spastic paraplegia, myopathies, myasthenia gravis, paramyotonia congentia, hyperkalemic periodic paralysis, hypokalemic periodic paralysis, alopecia, anxiety disorders, psychotic disorders, mania, paranoia, seasonal affective disorder, panic disorder, obsessive compulsive disorder (OCD), phobias, autism, Aspergers Syndrome, Retts syndrome, disintegrative disorder, attention deficit disorder, aggressivity, impulse control disorders, thrombosis, pre clampsia, congestive cardiac failure, cardiac arrest, Freidrich's ataxia, Spinocerebellear ataxia, myelopathy, radiculopathy, systemic lupus erythamatosis, granulomatous disease, olivo-ponto-cerebellar atrophy, spinocerebellar ataxia, episodic ataxia, myokymia, progressive pallidal atrophy, progressive supranuclear palsy and spasticity, traumatic brain injury, cerebral oedema, hydrocephalus injury, spinal cord injury, anorexia nervosa, bulimia, Prader-Willi syndrome, obesity, optic neuritis, cataract, retinal haemorrhage, ischaemic retinopathy, retinitis pigmentosa, acute and chronic glaucoma, macular degeneration, retinal artery occlusion, Chorea, Huntington's chorea, cerebral edema, proctitis, post-herpetic neuralgia, eudynia, heat sensitivity, sarcoidosis, irritable bowel syndrome, Tourette syndrome, Lesch-Nyhan Syndrome, Brugado syndrome, Liddle syndrome, Crohns disease, multiple sclerosis and the pain associated with multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS), disseminated sclerosis, diabetic neuropathy, peripheral neuropathy, charcot marie tooth syndrome, arthritic, rheumatoid arthritis, osteoarthritis, chondrocalcinosis, atherosclerosis, paroxysmal dystonia, myasthenia syndromes, myotonia, myotonic dystrophy, muscular dystrophy, malignant hyperthermia, cystic fibrosis, pseudoaldosteronism, rhabdomyolysis, mental handicap, hypothyroidism, bipolar depression, anxiety, schizophrenia, sodium channel toxin related illnesses, familial erythromelalgia, primary erythromelalgia, rectal pain, cancer, epilepsy, partial and general tonic seizures, febrile seizures, absence seizures (petit mal), myoclonic seizures, atonic seizures, clonic seizures, Lennox Gastaut, West Syndome (infantile spasms), multiresistant seizures, seizure prophylaxis (anti-epileptogenic), familial Mediterranean fever syndrome, gout, restless leg syndrome, arrhythmias, fibromyalgia, neuroprotection under ischaemic conditions caused by stroke or neural trauma, tachy-arrhythmias, atrial fibrillation and ventricular fibrillation and as a general or local anaesthetic.
  • As used herein, the term “pain” refers to all categories of pain and is recognized to include, but is not limited to, neuropathic pain, inflammatory pain, nociceptive pain, idiopathic pain, neuralgic pain, orofacial pain, burn pain, burning mouth syndrome, somatic pain, visceral pain, myofacial pain, dental pain, cancer pain, chemotherapy pain, trauma pain, surgical pain, post-surgical pain, childbirth pain, labor pain, chronic regional pain syndrome (CRPS), reflex sympathetic dystrophy, brachial plexus avulsion, neurogenic bladder, acute pain (e.g., musculoskeletal and post-operative pain), chronic pain, persistent pain, peripherally mediated pain, centrally mediated pain, chronic headache, migraine headache, familial hemiplegic migraine, conditions associated with cephalic pain, sinus headache, tension headache, phantom limb pain, peripheral nerve injury, pain following stroke, thalamic lesions, radiculopathy, HIV pain, post-herpetic pain, non-cardiac chest pain, irritable bowel syndrome and pain associated with bowel disorders and dyspepsia, and combinations thereof.
  • Furthermore, sodium channel blockers have clinical uses in addition to pain. The present invention therefore also relates to compounds, pharmaceutical compositions and methods of using the compounds and pharmaceutical compositions for the treatment of diseases or conditions such as cancer and pruritus (itch).
  • Pruritus, commonly known as itch, is a common dermatological condition. While the exact causes of pruritus are complex and incompletely understood, there has long been evidence that itch involves sensory neurons, especially C fibers, similar to those that mediate pain (Schmelz, M., et al., J. Neurosci. (1997), 17: 8003-8). In particular, it is believed that sodium influx through voltage-gated sodium channels is essential for the propagation of itch sensation from the skin. Transmission of the itch impulses results in the unpleasant sensation that elicits the desire or reflex to scratch.
  • Multiple causes and electrical pathways for eliciting itch are known. In humans, pruritus can be elicited by histamine or PAR-2 agonists such as mucunain that activate distinct populations of C fibers (Namer, B., et al., J. Neurophysiol. (2008), 100: 2062-9). A variety of neurotrophic peptides are known to mediate itch in animal models (Wang, H., and Yosipovitch, G., International Journal of Dermatology (2010), 49: 1-11). Itch can also be elicited by opioids, evidence of distinct pharmacology from that of pain responses.
  • There exists a complex interaction between itch and pain responses that arises in part from the overlapping sensory input from the skin (Ikoma, A., et al., Arch. Dermatol. (2003), 139: 1475-8) and also from the diverse etiology of both pain and pruritus. Pain responses can exacerbate itching by enhancing central sensitization or lead to inhibition of painful scratching. Particularly severe forms of chronic itch occur when pain responses are absent, as in the case of post-herpetic itch (Oaklander, A. L., et al., Pain (2002), 96: 9-12).
  • The compounds of the invention can also be useful for treating pruritus. The rationale for treating itch with inhibitors of voltage-gated sodium channels, especially NaV1.7, is as follows:
  • The propagation of electrical activity in the C fibers that sense pruritinergic stimulants requires sodium entry through voltage-gated sodium channels.
  • NaV1.7 is expressed in the C fibers and kerotinocytes in human skin (Zhao, P., et al., Pain (2008), 139: 90-105).
  • A gain of function mutation of NaV1.7 (L858F) that causes erythromelalgia also causes chronic itch (Li, Y., et al., Clinical and Experimental Dermatology (2009), 34: e313-e4).
  • Chronic itch can be alleviated with treatment by sodium channel blockers, such as the local anesthetic lidocaine (Oaklander, A. L., et al., Pain (2002), 96: 9-12; Villamil, A. G., et al., The American Journal of Medicine (2005), 118: 1160-3). In these reports, lidocaine was effective when administered either intravenously or topically (a Lidoderm patch). Lidocaine can have multiple activities at the plasma concentrations achieved when administered systemically, but when administered topically, the plasma concentrations are only about 1 μM (Center for Drug Evaluation and Research NDA 20-612). At these concentrations, lidocaine is selective for sodium channel block and inhibits spontaneous electrical activity in C fibers and pain responses in animal models (Xiao, W. H., and Bennett, G. J. Pain (2008), 137: 218-28). The types of itch or skin irritation, include, but are not limited to:
      • psoriatic pruritus, itch due to hemodyalisis, aguagenic pruritus, and itching caused by skin disorders (e.g., contact dermatitis), systemic disorders, neuropathy, psychogenic factors or a mixture thereof, itch caused by allergic reactions, insect bites, hypersensitivity (e.g., dry skin, acne, eczema, psoriasis), inflammatory conditions or injury;
      • itch associated with vulvar vestibulitis; and
      • skin irritation or inflammatory effect from administration of another therapeutic such as, for example, antibiotics, antivirals and antihistamines.
  • The compounds of the invention are also useful in treating certain cancers, such as hormone sensitive cancers, such as prostate cancer (adenocarcinoma), breast cancer, ovarian cancer, testicular cancer and thyroid neoplasia, in a mammal, preferably a human. The voltage gated sodium channels have been demonstrated to be expressed in prostate and breast cancer cells. Up-regulation of neonatal NaV1.5 occurs as an integral part of the metastatic process in human breast cancer and could serve both as a novel marker of the metastatic phenotype and a therapeutic target (Clin. Cancer Res. (2005), Aug. 1; 11(15): 5381-9). Functional expression of voltage-gated sodium channel alpha-subunits, specifically NaV1.7, is associated with strong metastatic potential in prostate cancer (CaP) in vitro. Voltage-gated sodium channel alpha-subunits immunostaining, using antibodies specific to the sodium channel alpha subunit was evident in prostatic tissues and markedly stronger in CaP vs non-CaP patients (Prostate Cancer Prostatic Dis., 2005; 8(3):266-73). See also Diss, J. K. J., et al., Mol. Cell. Neurosci. (2008), 37:537-547 and Kis-Toth, K., et al., The Journal of Immunology (2011), 187:1273-1280.
  • In consideration of the above, in one embodiment, the present invention provides a method for treating a mammal for, or protecting a mammal from developing, a sodium channel-mediated disease, especially pain, comprising administering to the mammal, especially a human, in need thereof, a therapeutically effective amount of a compound of the invention or a pharmaceutical composition comprising a therapeutically effective amount of a compound of the invention wherein the compound modulates the activity of one or more voltage-dependent sodium channels.
  • In another embodiment of the invention is a method of treating a disease or a condition in a mammal, preferably a human, wherein the disease or condition is selected from the group consisting of pain, depression, cardiovascular diseases, respiratory diseases, and psychiatric diseases, and combinations thereof, and wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount of an embodiment of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, or a pharmaceutical composition comprising a therapeutically effective amount of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, and a pharmaceutically acceptable excipient.
  • One embodiment of this embodiment is wherein the disease or condition is selected from the group consisting of acute pain, chronic pain, neuropathic pain, inflammatory pain, visceral pain, cancer pain, chemotherapy pain, trauma pain, surgical pain, post surgical pain, childbirth pain, labor pain, neurogenic bladder, ulcerative colitis, persistent pain, peripherally mediated pain, centrally mediated pain, chronic headache, migraine headache, sinus headache, tension headache, phantom limb pain, peripheral nerve injury, and combinations thereof.
  • Another embodiment of this embodiment is wherein the disease or condition is selected from the group consisting of pain associated with HIV, HIV treatment induced neuropathy, trigeminal neuralgia, post herpetic neuralgia, eudynia, heat sensitivity, tosarcoidosis, irritable bowel syndrome, Crohns disease, pain associated with multiple sclerosis (MS), amyotrophic lateral sclerosis (ALS), diabetic neuropathy, peripheral neuropathy, arthritic, rheumatoid arthritis, osteoarthritis, atherosclerosis, paroxysmal dystonia, myasthenia syndromes, myotonia, malignant hyperthermia, cystic fibrosis, pseudoaldosteronism, rhabdomyolysis, hypothyroidism, bipolar depression, anxiety, schizophrenia, sodium channel toxin related illnesses, familial erythromelalgia, primary erythromelalgia, familial rectal pain, cancer, epilepsy, partial and general tonic seizures, restless leg syndrome, arrhythmias, fibromyalgia, neuroprotection under ischaemic conditions caused by stroke or neural trauma, tachy arrhythmias, atrial fibrillation and ventricular fibrillation.
  • Another embodiment of the invention is a method of treating, but not preventing, pain in a mammal, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, or a pharmaceutical composition comprising a therapeutically effective amount of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, and a pharmaceutically acceptable excipient.
  • One embodiment of this embodiment is a method wherein the pain is selected from the group consisting of neuropathic pain, inflammatory pain, visceral pain, cancer pain, chemotherapy pain, trauma pain, surgical pain, post surgical pain, childbirth pain, labor pain, dental pain, chronic pain, persistent pain, peripherally mediated pain, centrally mediated pain, chronic headache, migraine headache, sinus headache, tension headache, phantom limb pain, peripheral nerve injury, trigeminal neuralgia, post herpetic neuralgia, eudynia, familial erythromelalgia, primary erythromelalgia, familial rectal pain or fibromyalgia, and combinations thereof.
  • Another embodiment of this embodiment is a method wherein the pain is associated with a disease or condition selected from HIV, HIV treatment induced neuropathy, heat sensitivity, tosarcoidosis, irritable bowel syndrome, Crohns disease, multiple sclerosis, amyotrophic lateral sclerosis, diabetic neuropathy, peripheral neuropathy, rheumatoid arthritis, osteoarthritis, atherosclerosis, paroxysmal dystonia, myasthenia syndromes, myotonia, malignant hyperthermia, cystic fibrosis, pseudoaldosteronism, rhabdomyolysis, hypothyroidism, bipolar depression, anxiety, schizophrenia, sodium channel toxin related illnesses, neurogenic bladder, ulcerative colitis, cancer, epilepsy, partial and general tonic seizures, restless leg syndrome, arrhythmias, ischaemic conditions caused by stroke or neural trauma, tachy arrhythmias, atrial fibrillation and ventricular fibrillation.
  • Another embodiment of the invention is the method of treating pain in a mammal, preferably a human, by the inhibition of ion flux through a voltage dependent sodium channel in the mammal, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount of an embodiment of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, or a pharmaceutical composition comprising a therapeutically effective amount of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, and a pharmaceutically acceptable excipient.
  • Another embodiment of the invention is the method of treating pruritus in a mammal, preferably a human, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount of an embodiment of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, or a pharmaceutical composition comprising a therapeutically effective amount of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, and a pharmaceutically acceptable excipient.
  • Another embodiment of the invention is the method of treating cancer in a mammal, preferably a human, wherein the method comprises administering to the mammal in need thereof a therapeutically effective amount of an embodiment of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, or a pharmaceutical composition comprising a therapeutically effective amount of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof, and a pharmaceutically acceptable excipient.
  • Another embodiment of the invention is the method of decreasing ion flux through a voltage dependent sodium channel in a cell in a mammal, wherein the method comprises contacting the cell with an embodiment of a compound of the invention, as set forth above, as a stereoisomer, enantiomer or tautomer thereof or mixtures thereof, or a pharmaceutically acceptable salt, solvate or prodrug thereof.
  • Another embodiment of the invention is the method of selectively inhibiting a first voltage-gated sodium channel over a second voltage-gated sodium channel in a mammal, wherein the method comprises administering to the mammal an inhibitory amount of a compound of formula (I), or an embodiment of a compound of formula (I).
  • Another embodiment of the invention is the method of selectively inhibiting NaV1.7 in a mammal or a mammalian cell as compared to NaV1.5, wherein the method comprises administering to the mammal in need thereof an inhibitory amount of a compound of formula (I) or an embodiment of an embodiment thereof.
  • For each of the above embodiments described related to treating diseases and conditions in a mammal, the present invention also contemplates relatedly a compound of formula I or an embodiment thereof for the use as a medicament in the treatment of such diseases and conditions.
  • For each of the above embodiments described related to treating diseases and conditions in a mammal, the present invention also contemplates relatedly the use of a compound of formula I or an embodiment thereof for the manufacture of a medicament for the treatment of such diseases and conditions.
  • Another embodiment of the invention is a method of using the compounds of formula (I) as standards or controls in in vitro or in vivo assays in determining the efficacy of test compounds in modulating voltage-dependent sodium channels.
  • In another embodiment of the invention, the compounds of formula (I) are isotopically-labeled by having one or more atoms therein replaced by an atom having a different atomic mass or mass number. Such isotopically-labeled (i.e., radiolabelled) compounds of formula (I) are considered to be within the scope of this invention. Examples of isotopes that can be incorporated into the compounds of formula (I) include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, sulfur, fluorine, chlorine, and iodine, such as, but not limited to, 2H, 3H, 11C, 13C, 14C, 13N, 15N, 15O, 17O, 18O, 31P, 32P, 35S, 8F, 36Cl, 123I, and 125I, respectively. These isotopically-labeled compounds would be useful to help determine or measure the effectiveness of the compounds, by characterizing, for example, the site or mode of action on the sodium channels, or binding affinity to pharmacologically important site of action on the sodium channels, particularly NaV1.7. Certain isotopically-labeled compounds of formula (I), for example, those incorporating a radioactive isotope, are useful in drug and/or substrate tissue distribution studies. The radioactive isotopes tritium, i.e. 3H, and carbon-14, i.e., 14C, are particularly useful for this purpose in view of their ease of incorporation and ready means of detection.
  • Substitution with heavier isotopes such as deuterium, i.e. 2H, may afford certain therapeutic advantages resulting from greater metabolic stability, for example, increased in vivo half-life or reduced dosage requirements, and hence may be preferred in some circumstances.
  • Substitution with positron emitting isotopes, such as 11C, 18F, 15O and 13N, can be useful in Positron Emission Topography (PET) studies for examining substrate receptor occupancy. Isotopically-labeled compounds of formula (I) can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described in the Examples as set out below using an appropriate isotopically-labeled reagent in place of the non-labeled reagent previously employed.
    • Testing Compounds
  • The assessment of the compounds of the invention in mediating, especially inhibiting, the sodium channel ion flux can be determined using the assays described hereinbelow. Alternatively, the assessment of the compounds in treating conditions and diseases in humans may be established in industry standard animal models for demonstrating the efficacy of compounds in treating pain. Animal models of human neuropathic pain conditions have been developed that result in reproducible sensory deficits (allodynia, hyperalgesia, and spontaneous pain) over a sustained period of time that can be evaluated by sensory testing. By establishing the degree of mechanical, chemical, and temperature induced allodynia and hyperalgesia present, several physiopathological conditions observed in humans can be modeled allowing the evaluation of pharmacotherapies.
  • In rat models of peripheral nerve injury, ectopic activity in the injured nerve corresponds to the behavioural signs of pain. In these models, intravenous application of the sodium channel blocker and local anesthetic lidocaine can suppress the ectopic activity and reverse the tactile allodynia at concentrations that do not affect general behaviour and motor function (Mao, J. and Chen, L. L, Pain (2000), 87:7-17). Allometric scaling of the doses effective in these rat models, translates into doses similar to those shown to be efficacious in humans (Tanelian, D. L. and Brose, W. G., Anesthesiology (1991), 74(5):949-951). Furthermore, Lidoderm®, lidocaine applied in the form of a dermal patch, is currently an FDA approved treatment for post-herpetic neuralgia (Devers, A. and Glaler, B. S., Clin. J. Pain (2000), 16(3):205-8).
  • The present invention readily affords many different means for identification of sodium channel modulating agents that are useful as therapeutic agents. Identification of modulators of sodium channel can be assessed using a variety of in vitro and in vivo assays, e.g., measuring current, measuring membrane potential, measuring ion flux, (e.g., sodium or guanidinium), measuring sodium concentration, measuring second messengers and transcription levels, and using e.g., voltage-sensitive dyes, radioactive tracers, and patch-clamp electrophysiology.
  • One such protocol involves the screening of chemical agents for ability to modulate the activity of a sodium channel thereby identifying it as a modulating agent.
  • A typical assay described in Bean et al., J. General Physiology (1983), 83:613-642, and Leuwer, M., et al., Br. J. Pharmacol (2004), 141(1):47-54, uses patch-clamp techniques to study the behaviour of channels. Such techniques are known to those skilled in the art, and may be developed, using current technologies, into low or medium throughput assays for evaluating compounds for their ability to modulate sodium channel behaviour.
  • Throughput of test compounds is an important consideration in the choice of screening assay to be used. In some strategies, where hundreds of thousands of compounds are to be tested, it is not desirable to use low throughput means. In other cases, however, low throughput is satisfactory to identify important differences between a limited number of compounds. Often it will be necessary to combine assay types to identify specific sodium channel modulating compounds.
  • Electrophysiological assays using patch clamp techniques is accepted as a gold standard for detailed characterization of sodium channel compound interactions, and as described in Bean et al., op. cit. and Leuwer, M., et al., op. cit. There is a manual low-throughput screening (LTS) method which can compare 2-10 compounds per day; a recently developed system for automated medium-throughput screening (MTS) at 20-50 patches (i.e. compounds) per day; and a technology from Molecular Devices Corporation (Sunnyvale, Calif.) which permits automated high-throughput screening (HTS) at 1000-3000 patches (i.e. compounds) per day.
  • One automated patch-clamp system utilizes planar electrode technology to accelerate the rate of drug discovery. Planar electrodes are capable of achieving high-resistance, cells-attached seals followed by stable, low-noise whole-cell recordings that are comparable to conventional recordings. A suitable instrument is the PatchXpress 7000A (Axon Instruments Inc, Union City, Calif.). A variety of cell lines and culture techniques, which include adherent cells as well as cells growing spontaneously in suspension are ranked for seal success rate and stability. Immortalized cells (e.g. HEK and CHO) stably expressing high levels of the relevant sodium ion channel can be adapted into high-density suspension cultures.
  • Other assays can be selected which allow the investigator to identify compounds which block specific states of the channel, such as the open state, closed state or the resting state, or which block transition from open to closed, closed to resting or resting to open. Those skilled in the art are generally familiar with such assays.
  • Binding assays are also available. Designs include traditional radioactive filter based binding assays or the confocal based fluorescent system available from Evotec OAI group of companies (Hamburg, Germany), both of which are HTS.
  • Radioactive flux assays can also be used. In this assay, channels are stimulated to open with veratridine or aconitine and held in a stabilized open state with a toxin, and channel blockers are identified by their ability to prevent ion influx. The assay can use radioactive 22[Na] and 14[C] guanidinium ions as tracers. FlashPlate & Cytostar-T plates in living cells avoids separation steps and are suitable for HTS. Scintillation plate technology has also advanced this method to HTS suitability. Because of the functional aspects of the assay, the information content is reasonably good.
  • Yet another format measures the redistribution of membrane potential using the FLIPR system membrane potential kit (HTS) available from Molecular Dynamics (a division of Amersham Biosciences, Piscataway, N.J.). This method is limited to slow membrane potential changes. Some problems may result from the fluorescent background of compounds. Test compounds may also directly influence the fluidity of the cell membrane and lead to an increase in intracellular dye concentrations. Still, because of the functional aspects of the assay, the information content is reasonably good.
  • Sodium dyes can be used to measure the rate or amount of sodium ion influx through a channel. This type of assay provides a very high information content regarding potential channel blockers. The assay is functional and would measure Na+ influx directly. CoroNa Red, SBFI and/or sodium green (Molecular Probes, Inc. Eugene Oreg.) can be used to measure Na influx; all are Na responsive dyes. They can be used in combination with the FLIPR instrument. The use of these dyes in a screen has not been previously described in the literature. Calcium dyes may also have potential in this format.
  • In another assay, FRET based voltage sensors are used to measure the ability of a test compound to directly block Na influx. Commercially available HTS systems include the VIPR™ II FRET system (Life Technologies, or Aurora Biosciences Corporation, San Diego, Calif., a division of Vertex Pharmaceuticals, Inc.) which may be used in conjunction with FRET dyes, also available from Aurora Biosciences. This assay measures sub-second responses to voltage changes. There is no requirement for a modifier of channel function. The assay measures depolarization and hyperpolarizations, and provides ratiometric outputs for quantification. A somewhat less expensive MTS version of this assay employs the FLEXstation™ (Molecular Devices Corporation) in conjunction with FRET dyes from Aurora Biosciences. Other methods of testing the compounds disclosed herein are also readily known and available to those skilled in the art.
  • Modulating agents so identified are then tested in a variety of in vivo models so as to determine if they alleviate pain, especially chronic pain or other conditions such as cancer and pruritus (itch) with minimal adverse events. The assays described below in the Biological Assays Section are useful in assessing the biological activity of the instant compounds.
  • Typically, the efficacy of a compound of the invention is expressed by its IC50 value (“Inhibitory Concentration—50%”), which is the measure of the amount of compound required to achieve 50% inhibition of the activity of the target sodium channel over a specific time period. For example, representative compounds of the present invention have demonstrated IC50's ranging from less than 100 nanomolar to less than 10 micromolar in the patch voltage clamp NaV1.7 electrophysiology assay described herein.
  • In another aspect of the invention, the compounds of the invention can be used in in vitro or in vivo studies as exemplary agents for comparative purposes to find other compounds also useful in treatment of, or protection from, the various diseases disclosed herein.
  • Another aspect of the invention relates to inhibiting NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.5, NaV1.6, NaV1.7, NaV1.8, or NaV1.9 activity, preferably NaV1.7 activity, in a biological sample or a mammal, preferably a human, which method comprises administering to the mammal, preferably a human, or contacting said biological sample with a compound of formula (I) or a pharmaceutical composition comprising a compound of formula (I). The term “biological sample”, as used herein, includes, without limitation, cell cultures or extracts thereof; biopsied material obtained from a mammal or extracts thereof; and blood, saliva, urine, feces, semen, tears, or other body fluids or extracts thereof.
  • Inhibition of NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.5, NaV1.6, NaV1.7, NaV1.8, or NaV1.9 activity in a biological sample is useful for a variety of purposes that are known to one of skill in the art. Examples of such purposes include, but are not limited to, the study of sodium ion channels in biological and pathological phenomena; and the comparative evaluation of new sodium ion channel inhibitors.
  • The compounds of the invention (or stereoisomers, geometric isomers, tautomers, solvates, metabolites, isotopes, pharmaceutically acceptable salts, or prodrugs thereof) and/or the pharmaceutical compositions described herein which comprise a pharmaceutically acceptable excipient and one or more compounds of the invention, can be used in the preparation of a medicament for the treatment of sodium channel-mediated disease or condition in a mammal.
    • Combination Therapy
  • The compounds of the invention may be usefully combined with one or more other compounds of the invention or one or more other therapeutic agent or as any combination thereof, in the treatment of sodium channel-mediated diseases and conditions. For example, a compound of the invention may be administered simultaneously, sequentially or separately in combination with other therapeutic agents, including, but not limited to:
      • opiates analgesics, e.g., morphine, heroin, cocaine, oxymorphine, levorphanol, levallorphan, oxycodone, codeine, dihydrocodeine, propoxyphene, nalmefene, fentanyl, hydrocodone, hydromorphone, meripidine, methadone, nalorphine, naloxone, naltrexone, buprenorphine, butorphanol, nalbuphine and pentazocine;
      • non-opiate analgesics, e.g., acetomeniphen, salicylates (e.g., aspirin);
      • nonsteroidal antiinflammatory drugs (NSAIDs), e.g., ibuprofen, naproxen, fenoprofen, ketoprofen, celecoxib, diclofenac, diflusinal, etodolac, fenbufen, fenoprofen, flufenisal, flurbiprofen, ibuprofen, indomethacin, ketoprofen, ketorolac, meclofenamic acid, mefenamic acid, meloxicam, nabumetone, naproxen, nimesulide, nitroflurbiprofen, olsalazine, oxaprozin, phenylbutazone, piroxicam, sulfasalazine, sulindac, tolmetin and zomepirac;
      • anticonvulsants, e.g., carbamazepine, oxcarbazepine, lamotrigine, valproate, topiramate, gabapentin and pregabalin;
      • antidepressants such as tricyclic antidepressants, e.g., amitriptyline, clomipramine, despramine, imipramine and nortriptyline;
      • COX-2 selective inhibitors, e.g., celecoxib, rofecoxib, parecoxib, valdecoxib, deracoxib, etoricoxib, and lumiracoxib;
      • alpha-adrenergics, e.g., doxazosin, tamsulosin, clonidine, guanfacine, dexmetatomidine, modafinil, and 4-amino-6,7-dimethoxy-2-(5-methane sulfonamido-1,2,3,4-tetrahydroisoquinol-2-yl)-5-(2-pyridyl) quinazoline;
      • barbiturate sedatives, e.g., amobarbital, aprobarbital, butabarbital, butabital, mephobarbital, metharbital, methohexital, pentobarbital, phenobartital, secobarbital, talbutal, theamylal and thiopental;
      • tachykinin (NK) antagonist, particularly an NK-3, NK-2 or NK-1 antagonist, e.g., (αR,9R)-7-[3,5-bis(trifluoromethyl)benzyl)]-8,9,10,11-tetrahydro-9-methyl-5-(4-methylphenyl)-7H-[1,4]diazocino[2,1-g][1,7]-naphthyridine-6-13-dione (TAK-637), 5-[[2R,3S)-2-[(1R)-1-[3,5-bis(trifluoromethylphenyl]ethoxy-3-(4-fluorophenyl)-4-morpholinyl]-methyl]-1,2-dihydro-3H-1,2,4-triazol-3-one (MK-869), aprepitant, lanepitant, dapitant or 3-[[2-methoxy5-(trifluoromethoxy)phenyl]-methylamino]-2-phenylpiperidine (2S,3S);
      • coal-tar analgesics, in particular paracetamol;
      • serotonin reuptake inhibitors, e.g., paroxetine, sertraline, norfluoxetine (fluoxetine desmethyl metabolite), metabolite demethylsertraline, ′3 fluvoxamine, paroxetine, citalopram, citalopram metabolite desmethylcitalopram, escitalopram, d,l-fenfluramine, femoxetine, ifoxetine, cyanodothiepin, litoxetine, dapoxetine, nefazodone, cericlamine, trazodone and fluoxetine;
      • noradrenaline (norepinephrine) reuptake inhibitors, e.g., maprotiline, lofepramine, mirtazepine, oxaprotiline, fezolamine, tomoxetine, mianserin, buproprion, buproprion metabolite hydroxybuproprion, nomifensine and viloxazine (Vivalan®)), especially a selective noradrenaline reuptake inhibitor such as reboxetine, in particular (S,S)-reboxetine, and venlafaxine duloxetine neuroleptics sedative/anxiolytics;
      • dual serotonin-noradrenaline reuptake inhibitors, such as venlafaxine, venlafaxine metabolite O-desmethylvenlafaxine, clomipramine, clomipramine metabolite desmethylclomipramine, duloxetine, milnacipran and imipramine;
      • acetylcholinesterase inhibitors such as donepezil;
      • 5-HT3 antagonists such as ondansetron;
      • metabotropic glutamate receptor (mGluR) antagonists;
      • local anaesthetic such as mexiletine and lidocaine;
      • corticosteroid such as dexamethasone;
      • antiarrhythimics, e.g., mexiletine and phenytoin;
      • muscarinic antagonists, e.g., tolterodine, propiverine, tropsium t chloride, darifenacin, solifenacin, temiverine and ipratropium;
      • cannabinoids;
      • vanilloid receptor agonists (e.g., resinferatoxin) or antagonists (e.g., capsazepine);
      • sedatives, e.g., glutethimide, meprobamate, methaqualone, and dichloralphenazone;
      • anxiolytics such as benzodiazepines,
      • antidepressants such as mirtazapine,
      • topical agents (e.g., lidocaine, capsacin and resiniferotoxin);
      • muscle relaxants such as benzodiazepines, baclofen, carisoprodol, chlorzoxazone, cyclobenzaprine, methocarbamol and orphrenadine;
      • anti-histamines or H1 antagonists;
      • NMDA receptor antagonists;
      • 5-HT receptor agonists/antagonists;
      • PDEV inhibitors;
      • Tramadol®;
      • cholinergic (nicotinc) analgesics;
      • alpha-2-delta ligands;
      • prostaglandin E2 subtype antagonists;
      • leukotriene B4 antagonists;
      • 5-lipoxygenase inhibitors; and
      • 5-HT3 antagonists.
  • Sodium channel-mediated diseases and conditions that may be treated and/or prevented using such combinations include but not limited to, pain, central and peripherally mediated, acute, chronic, neuropathic as well as other diseases with associated pain and other central nervous disorders such as epilepsy, anxiety, depression and bipolar disease; or cardiovascular disorders such as arrhythmias, atrial fibrillation and ventricular fibrillation; neuromuscular disorders such as restless leg syndrome and muscle paralysis or tetanus; neuroprotection against stroke, neural trauma and multiple sclerosis; and channelopathies such as erythromyalgia and familial rectal pain syndrome.
  • As used herein “combination” refers to any mixture or permutation of one or more compounds of the invention and one or more other compounds of the invention or one or more additional therapeutic agent. Unless the context makes clear otherwise, “combination” may include simultaneous or sequentially delivery of a compound of the invention with one or more therapeutic agents. Unless the context makes clear otherwise, “combination” may include dosage forms of a compound of the invention with another therapeutic agent. Unless the context makes clear otherwise, “combination” may include routes of administration of a compound of the invention with another therapeutic agent. Unless the context makes clear otherwise, “combination” may include formulations of a compound of the invention with another therapeutic agent. Dosage forms, routes of administration and pharmaceutical compositions include, but are not limited to, those described herein.
  • The invention will be more fully understood by reference to the following examples. They should not, however, be construed as limiting the scope of the invention.
    • EXAMPLES
  • These examples serve to provide guidance to a skilled artisan to prepare and use the compounds, compositions and methods of the invention. While a particular embodiment of the present invention are described, the skilled artisan will appreciate that various changes and modifications can be made without departing from the spirit and scope of the inventions.
  • The chemical reactions in the examples described can be readily adapted to prepare a number of other compounds of the invention, and alternative methods for preparing the compounds of this invention are deemed to be within the scope of this invention. For example, the synthesis of non-exemplified compounds according to the invention can be successfully performed by modifications apparent to those skilled in the art, for example, by appropriately protecting interfering groups by utilizing other suitable reagents known in the art other than those described, and/or by making routine modifications of reaction conditions.
  • In the examples below, unless otherwise indicated all temperatures are set forth in degrees Celsius. Commercially available reagents were purchased from suppliers such as Aldrich Chemical Company, Lancaster, TCI or Maybridge and were used without further purification unless otherwise indicated. The reactions set forth below were done under a positive pressure of nitrogen or argon or with a drying tube (unless otherwise stated) in anhydrous solvents, and the reaction flasks were typically fitted with rubber septa for the introduction of substrates and reagents via syringe. Glassware was oven dried and/or heat dried. 1H NMR spectra were obtained in deuterated CDCl3, d6-DMSO, CH3OD or d6-acetone solvent solutions (reported in ppm) using or trimethylsilane (TMS) or residual non-deuterated solvent peaks as the reference standard. When peak multiplicities are reported, the following abbreviates are used: s (singlet), d (doublet), t (triplet), q (quartet), m (multiplet), br (broad), dd (doublet of doublets), dt (doublet of triplets). Coupling constants, when given, are reported in Hz (Hertz).
  • All abbreviations used to describe reagents, reaction conditions or equipment are intended to be consistent with the definitions set forth in the “List of standard abbreviates and acronyms”. The chemical names of discrete compounds of the invention were obtained using the structure naming feature of ChemDraw naming program.
    • Abbreviations
    • MeCN Acetonitrile
    • EtOAc Ethyl acetate
    • DCE Dichloroethane
    • DCM Dichloromethane
    • DIPEA Diisopropylethylamine
    • DEA Diethylamine
    • DMAP 4-dimethylaminopyridine
    • DMF N,N-Dimethylformamide
    • DMSO Dimethyl sulfoxide
    • FA Formic acid
    • IPA Isopropyl alcohol
    • TFA Trifluoroacetic acid
    • EDCI 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride
    • HCl Hydrochloric acid
    • HPLC High Pressure Liquid Chromatography
      • LCMS Liquid Chromatography Mass Spectrometry
      • MeOH Methanol
      • NMP N-methyl-2-pyrrolidone
      • RPHPLC Reverse phase high pressure liquid chromatography
      • RT Retention time
      • SFC Supercritical Fluid Chromatography
      • THF Tetrahydrofuran
      • TEA Triethylamine
    Example 1
  • Figure US20200115354A1-20200416-C00105
    • 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00106
    • Step 1
  • Figure US20200115354A1-20200416-C00107
    • 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Lithium Bis(trimethylsilyl)amide (1.0 M, 4.8 mL, 4.8 mmol) was added dropwise to a solution of N-[(2,4-dimethoxyphenyl)methyl]thiazole-2-amine (1.0 g, 4.0 mmol) in THF (30 mL) at room temperature. After stirring for 30 minutes, a solution of 5-chloro-2,4-difluoro-benzenesulfonyl chloride (987 mg, 4.0 mmol) in THF (10 mL) was added. The reaction was stirred at room temperature for 3 hours, then concentrated. The crude product was purified by column chromatography (iPrOAc/Heptane) to provide 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide (850 mg, 46% yield) as a white solid. LCMS (ESI) m/z: 461.0 [M+H]+. 1H NMR (400 MHz, Chloroform-d) δ 7.92 (dd, J=7.9, 7.0 Hz, 1H), 7.42 (d, J=3.6 Hz, 1H), 7.19 (d, J=8.3 Hz, 1H), 7.04-6.96 (m, 2H), 6.39-6.33 (m, 2H), 5.18 (s, 2H), 3.76 (s, 3H), 3.72 (s, 3H).
    • Step 2
  • Figure US20200115354A1-20200416-C00108
    • tert-butyl((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)carbamate
  • 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide (850 mg, 1.8 mmol) was dissolved in DMF (18 mL). TEA (1.3 mL, 9.2 mmol) and tert-butyl N-[(1S,2S)-2-aminocyclohexyl]carbamate (395 mg, 1.8 mmol) were added sequentially. The reaction was stirred at room temperature for 20 hours, then quenched by the addition of saturated aqueous NaHCO3 (50 mL) and DCM (50 mL). The mixture was separated and the aqueous layer was extracted with DCM (3×50 mL). The combined organic fractions were dried with NaSO4, filtered, and concentrated. The crude product was purified by column chromatography (iPrOAc/Heptane) to provide tert-butyl((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)carbamate (900 mg, 75%) as a white solid. LCMS (ESI) m/z: 677.1 [M+H]+. 1H NMR (400 MHz, Chloroform-d) δ 7.70 (d, J=7.2 Hz, 1H), 7.38 (d, J=3.6 Hz, 1H), 7.21 (dt, J=8.5, 0.9 Hz, 1H), 6.95 (d, J=3.6 Hz, 1H), 6.37 (d, J=7.4 Hz, 2H), 6.20 (d, J=12.7 Hz, 1H), 5.92 (d, J=6.4 Hz, 1H), 4.52 (d, J=8.6 Hz, 1H), 5.19 (s, 2H), 3.75 (s, 3H), 3.75 (s, 3H), 3.63 (d, J=10.4 Hz, 1H), 3.04-2.89 (m, 1H), 2.19-2.02 (m, 2H), 1.80 (t, J=14.4 Hz, 2H), 1.41 (s, 9H), 1.38-1.24 (m, 4H).
  • Step 3
  • Figure US20200115354A1-20200416-C00109
    • 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Trifluoroacetic acid (0.34 mL, 4.59 mmol) was added dropwise to a solution of tert-butyl((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)carbamate (300 mg, 0.46 mmol) in DCM (1.5 mL) at room temperature. After 16 hours, the reaction was concentrated. The crude product (185 mg) was used without further purification in the subsequent step. A small portion was purified by HPLC to give 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide as a white solid. LCMS (ESI) m/z: 405.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.80 (s, 1H), 7.57 (d, J=7.3 Hz, 1H), 6.98 (d, J=3.9 Hz, 1H), 6.71 (d, J=12.8 Hz, 1H), 6.50 (d, J=3.8 Hz, 1H), 5.70 (dd, J=9.7, 2.0 Hz, 1H), 3.43-3.32 (m, 1H), 3.31-3.11 (m, 1H), 2.07 (s, 1H), 1.98 (d, J=12.4 Hz, 1H), 1.91-1.83 (m, 1H), 1.70 (d, J=13.0 Hz, 1H), 1.63 (d, J=12.2 Hz, 1H), 1.44-1.23 (m, 3H), 1.22 (dd, J=14.5, 10.7 Hz, 1H).
    • Example 2
  • Figure US20200115354A1-20200416-C00110
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (185 mg, 0.46 mmol) was suspended in MeCN (4.5 mL). Formaldehyde (37% w/w in H2O, 0.2 mL, 2.7 mmol) and NaBH(OAc)3 (970 mg, 4.5 mmol) were added sequentially at room temperature. After 4 hours, the reaction was concentrated and purified by HPLC to give 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (42.1 mg, 21%) as a white solid. LCMS (ESI) m/z: 433.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.56 (d, J=7.3 Hz, 1H), 7.11-6.94 (m, 1H), 6.62 (d, J=12.6 Hz, 1H), 6.56 (d, J=4.0 Hz, 1H), 6.52 (s, 1H), 5.65 (s, 1H), 3.24-3.18 (m, OH), 2.62-2.53 (m, 1H), 2.17 (s, 7H), 1.88-1.71 (m, 2H), 1.60 (d, J=13.3 Hz, 1H), 1.36 (dd, J=14.6, 11.0 Hz, 1H), 1.30-1.02 (m, 4H).
    • Example 3
  • Figure US20200115354A1-20200416-C00111
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide 2,2,2-trifluoroacetate
  • Sodium hydride (60 mass %) in mineral oil (23.6 mg, 0.59 mmol) was added to a solution of tert-butyl N-[(1S,2S)-2-[2-chloro-4-[(2,4-dimethoxyphenyl)methyl-thiazol-2-yl-sulfamoyl]-5-fluoro-anilino]cyclohexyl]carbamate (387 mg, 0.59 mmol) in N,N-dimethylformamide (5 mL). The mixture was stirred at room temperature for 10 min then iodomethane (83.8 mg, 0.59 mmol) was added. The reaction was stirred at room temperature for 2 h. The reaction was then quenched by addition of 15 mL of water. The resulting solid was collected by filtration, washed with water and dried in vacuo for 12 h. The solid was dissolved in dichloromethane (3 mL) and trifluoroacetic acid (0.45 mL, 5.9 mmol) was added. The reaction was stirred at room temperature for 1 h, concentrated and purified by reverse phase HPLC to afford 22.9 mg (9%) of 5-chloro-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide 2,2,2-trifluoroacetate as a white solid. LCMS (ESI) m/z: 419.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.67 (d, J=7.4 Hz, 1H), 7.11 (dd, J=11.8, 4.4 Hz, 1H), 7.05-6.96 (m, 1H), 6.58-6.51 (m, 1H), 3.29 (s, 3H), 3.13-3.05 (m, 1H), 2.68 (d, J=7.6 Hz, 2H), 2.50 (d, J=13.1 Hz, 3H), 2.18 (d, J=9.1 Hz, 1H), 1.69 (ddd, J=31.0, 24.2, 10.2 Hz, 2H), 1.58 (s, 1H), 1.51 (d, J=11.0 Hz, 1H), 1.22 (dd, J=13.7, 5.0 Hz, 2H).
    • Example 4
  • Figure US20200115354A1-20200416-C00112
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide 2,2,2-trifluoroacetate
  • Following the procedure described in Example 3 and making non-critical variations as required to replace iodomethane with iodoethane, 5-chloro-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide 2,2,2-trifluoroacetate was obtained as a white solid. LCMS (ESI) m/z: 433.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.58 (d, J=7.3 Hz, 1H), 7.02 (d, J=4.0 Hz, 1H), 6.74 (d, J=12.8 Hz, 1H), 6.55 (d, J=4.0 Hz, 1H), 5.75 (d, J=9.1 Hz, 1H), 3.28 (d, J=6.6 Hz, 1H), 3.08 (s, 1H), 2.86 (dp, J=28.0, 6.5 Hz, 1H), 2.09 (d, J=13.0 Hz, 1H), 1.96-1.87 (m, 1H), 1.73 (d, J=12.4 Hz, 1H), 1.62 (d, J=12.3 Hz, 1H), 1.45-1.16 (m, 3H), 1.10 (t, J=7.1 Hz, 3H).
    • Example 5
  • Figure US20200115354A1-20200416-C00113
    • 4-(((1S,2S)-2-(benzylamino)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide 2,2,2-trifluoroacetate
  • Following the procedure described in Example 3 and making non-critical variations as required to replace iodomethane with benzyl bromide, 4-(((1S,2S)-2-(benzylamino)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide 2,2,2-trifluoroacetate was obtained as a white solid. LCMS m/z: 495.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.58 (d, J=7.3 Hz, 1H), 7.31-7.14 (m, 6H), 6.78-6.69 (m, 2H), 5.74 (d, J=7.2 Hz, 1H), 3.84 (d, J=13.5 Hz, 1H), 3.70 (d, J=13.5 Hz, 1H), 3.41-3.31 (m, 1H), 3.29 (s, 1H), 2.62 (s, 1H), 2.09 (s, 1H), 1.95 (d, J=12.5 Hz, 1H), 1.70 (s, 1H), 1.61 (d, J=13.4 Hz, 1H), 1.35 (t, J=11.9 Hz, 1H), 1.20 (t, J=10.9 Hz, 3H).
    • Example 6
  • Figure US20200115354A1-20200416-C00114
    • 4-(((1S,2S)-2-(benzyl(ethyl)amino)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Sodium triacetoxyborohydride (78.5 mg 0.37 mmol) was added to a mixture of 4-[[(1S,2S)-2-aminocyclohexyl]amino]-5-chloro-2-fluoro-N-thiazol-2-yl-benzenesulfonamide (30 mg, 0.07 mmol) and benzaldehyde (9.4 mg, 0.09 mmol) in 1,2-dichloroethane (2 mL) at 70° C. The mixture was stirred at 70° C. for 16 h. The reaction was then cooled to room temperature, quenched by addition of 1 mL of methanol, concentrated and purified by reverse phase HPLC to give 1.4 mg of 4-(((1S,2S)-2-(benzyl(ethyl)amino)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide as a white solid. LCMS (ESI) m/z: 523.1 [M+H]+.
    • Example 7 & Example 8
  • Figure US20200115354A1-20200416-C00115
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1R,2R)-2-(dimethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00116
    • Step 1:
  • Figure US20200115354A1-20200416-C00117
    • Racemic (trans)-5-chloro-N-(2,4-dimethoxybenzyl)-4-((2-(dimethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & racemic (trans)-5-chloro-N-(2,4-dimethoxybenzyl)-2-((2-(dimethylamino)cyclohexyl)oxy)-4-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of racemic (trans)-2-(dimethylamino)cyclohexanol (1.0 g, 5.93 mmol) in anhydrous THF (8.5 mL) at 0 HC was added NaH (60%, 0.36 g, 8.9 mmol). The reaction mixture was stirred at 0° C. for 0.5 h. 5-Chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide (2.87 g, 6.23 mmol) at 0° C. was added portionwise. The reaction mixture was stirred at room temperature for an additional 2 h. Sat. aq. NH4Cl (50 mL) was added and extracted with EtOAc (200 mL). The organic layer was washed with water (50 mL), brine (80 mL×2), dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (DCM/MeOH=20:1) to give a mixture of title compounds (3 g, rac-3A:rac-3B˜5:4) as yellow oil. LCMS (ESI) m/z: 584 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00118
    • Racemic (trans)-5-chloro-4-((2-(dimethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & racemic (trans)-5-chloro-2-((2-(dimethylamino)cyclohexyl)oxy)-4-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of racemic (trans)-5-chloro-N-(2,4-dimethoxybenzyl)-4-((2-(dimethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide and racemic (trans)-5-chloro-N-(2,4-dimethoxybenzyl)-2-((2-(dimethylamino)cyclohexyl)oxy)-4-fluoro-N-(thiazol-2-yl)benzenesulfonamide (1.0 g, 1.71 mmol) in DCM (10 mL) was added trifluoroacetic acid (1.31 mL, 17.12 mmol). The reaction mixture was stirred at room temperature for 2 h. DCM (20 mL) was added and the mixture was basified with NH4OH to pH 12. The organic layer was dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 25-55%/0.05% NH4OH in water) to give racemic (trans)-5-chloro-4-((2-(dimethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (100 mg, first peak) as a white solid and racemic (trans)-5-chloro-2-((2-(dimethylamino)cyclohexyl)oxy)-4-fluoro-N-(thiazol-2-yl)benzenesulfonamide (62 mg, second peak) as a white solid. First peak: LCMS (ESI) m/z: 434 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.75 (d, J=7.2 Hz, 1H), 7.42 (d, J=12.0 Hz, 1H), 7.22 (d, J=4.4 Hz, 1H), 6.78 (d, J=4.0 Hz, 1H), 4.75-4.61 (m, 1H), 2.88-2.76 (m, 1H), 2.36 (s, 6H), 2.09-1.99 (m, 1H), 1.89-1.77 (m, 1H), 1.75-1.56 (m, 2H), 1.40-1.24 (m, 4H). F NMR (400 MHz, DMSO-d6) δ −107.48 (s, 1F). Second peak: LCMS (ESI) m/z: 434 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.78 (d, J=8.8 Hz, 1H), 7.34 (d, J=10.8 Hz, 1H), 7.07 (d, J=3.6 Hz, 1H), 6.64 (d, J=4.0 Hz, 1H), 4.60-4.51 (m, 1H), 3.61-3.51 (m, 1H), 2.85 (s, 6H), 2.15-2.05 (m, 1H), 1.85-1.71 (m, 2H), 1.49-1.35 (m, 5H). F NMR (400 MHz, DMSO-d6) δ −109.10 (s, 1F).
    • Step 3:
  • Figure US20200115354A1-20200416-C00119
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1R,2R)-2-(dimethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Racemic (trans)-5-chloro-4-((2-(dimethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (0.6 g) was separated by using chiral SFC (Chiralpak C2 (250 mm*30 mm, 10 um), Supercritical CO2/EtOH+0.1% NH4OH=40/40; 60 mL/min) to give 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (167 mg, first peak) as a white solid and 5-chloro-4-(((1R,2R)-2-(dimethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (164 mg, second peak) as a white solid. Absolute configuration was arbitrarily assigned to each enantiomer. Example 1: LCMS (ESI) m/z: 434 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.74 (d, J=7.6 Hz, 1H), 7.41 (d, J=12.0 Hz, 1H), 7.21 (d, J=4.4 Hz, 1H), 6.77 (d, J=4.4 Hz, 1H), 4.72-4.59 (m, 1H), 2.85-2.77 (m, 1H), 2.34 (s, 6H), 2.09-1.93 (m, 1H), 1.89-1.77 (m, 1H), 1.75-1.56 (m, 2H), 1.40-1.15 (m, 4H). Example 2: LCMS (ESI) m/z: 434 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.74 (d, J=7.6 Hz, 1H), 7.40 (d, J=12.0 Hz, 1H), 7.20 (d, J=4.4 Hz, 1H), 6.76 (d, J=4.4 Hz, 1H), 4.72-4.59 (m, 1H), 2.83-2.73 (m, 1H), 2.34 (s, 6H), 2.07-1.99 (m, 1H), 1.88-1.80 (m, 1H), 1.71-1.57 (m, 2H), 1.42-1.17 (m, 4H).
    • Example 9 & Example 10
  • Figure US20200115354A1-20200416-C00120
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclopentyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1R,2R)-2-(dimethylamino)cyclopentyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00121
    • Step 1:
  • Figure US20200115354A1-20200416-C00122
    • Racemic (trans)-2-(dimethylamino)cyclopentanol
  • A mixture of 6-oxabicyclo[3.1.0]hexane (1.0 g, 11.89 mmol) and 33% aqueous solution of dimethylamine (7.3 mL, 47.55 mmol) was stirred at room temperature for 16 h. Water (15 mL) was added and extracted with EtOAc (20 mL×3). The combined organic layers were dried over anhydrous MgSO4, filtered and concentrated in vacuo to give the title compound (1.13 g, crude) as colorless oil that required no further purification. 1H NMR (400 MHz, CDCl3) δ 4.11-4.01 (m, 1H), 2.52-2.42 (m, 1H), 2.28 (s, 6H), 2.00-1.88 (m, 1H), 1.87-1.76 (m, 1H), 1.75-1.40 (m, 4H).
    • Step 2:
  • Figure US20200115354A1-20200416-C00123
    • Racemic (trans)-5-chloro-N-(2,4-dimethoxybenzyl)-4-((2-(dimethylamino)cyclopentyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of racemic (trans)-2-(dimethylamino)cyclopentanol (0.4 g, 3.1 mmol) in anhydrous DMF (13 mL) at 0° C. was added NaH (60%, 0.18 g, 4.5 mmol). The reaction mixture was stirred at 0° C. for 0.5 h. 5-Chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide (1.3 g, 2.82 mmol) at 0° C. was added portionwise. The reaction mixture was stirred at room temperature for an additional 16 h. EtOAc (50 mL) was added and washed with water (15 mL×3). The organic layer was dried over anhydrous Na2SO4, filtered and concentrated in vacuo to give the title compound (1.08 g, crude) as yellow oil that required no further purification. LCMS (ESI) m/z: 570 [M+H]+.
    • Step 3:
  • Figure US20200115354A1-20200416-C00124
    • Racemic (trans)-5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclopentyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of racemic (trans)-5-chloro-N-(2,4-dimethoxybenzyl)-4-((2-(dimethylamino)cyclopentyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (1.08 g, 1.89 mmol) in DCM (5 mL) was added trifluoroacetic acid (1.41 mL, 18.94 mmol). The reaction mixture was stirred at room temperature for 12 h and concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 10-40%/0.05% HCl in water) to give the title compound (240 mg, 30%) as colorless oil. LCMS (ESI) m/z: 420 [M+H]+.
    • Step 4:
  • Figure US20200115354A1-20200416-C00125
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclopentyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1R,2R)-2-(dimethylamino)cyclopentyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Racemic (trans)-5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclopentyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (240 mg) was separated by using chiral SFC (Chiralpak C2 (250 mm*30 mm, 10 um), Supercritical CO2/MeOH+0.1% NH4OH=60/40; 60 mL/min) to give 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclopentyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (49 mg, first peak) as a yellow solid and 5-chloro-4-(((1R,2R)-2-(dimethylamino)cyclopentyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (102 mg, second peak) as a yellow solid. Absolute configuration was arbitrarily assigned to each enantiomer. Example 3: LCMS (ESI) m/z: 420 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.75 (d, J=7.6 Hz, 1H), 7.32 (d, J=12.0 Hz, 1H), 7.22 (d, J=4.4 Hz, 1H), 6.79 (d, J=4.4 Hz, 1H), 4.90-4.83 (m, 1H), 3.10-3.01 (m, 1H), 2.31 (s, 6H), 2.17-2.04 (m, 1H), 2.00-1.89 (m, 1H), 1.75-1.50 (m, 4H). Example 4: LCMS (ESI) m/z: 420 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.75 (d, J=7.6 Hz, 1H), 7.31 (d, J=12.4 Hz, 1H), 7.22 (d, J=4.0 Hz, 1H), 6.79 (d, J=4.0 Hz, 1H), 4.84-4.77 (m, 1H), 3.02-2.94 (m, 1H), 2.27 (s, 6H), 2.15-2.02 (m, 1H), 1.97-1.87 (m, 1H), 1.72-1.47 (m, 4H).
    • Example 11
  • Figure US20200115354A1-20200416-C00126
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00127
    • Step 1:
  • Figure US20200115354A1-20200416-C00128
    • 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • To a solution of 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide (130 mg, 0.31 mmol, US201210182, preparation 110) and K2CO3 (127 mg, 0.92 mmol) in DMF (2 mL) was added (1S,2S)—N1,N1-dimethylcyclohexane-1,2-diamine (43 mg, 0.31 mmol). The reaction mixture was stirred at room temperature for 16 h. The mixture was diluted with EtOAc (30 mL), washed with water (20 mL), brine (20 mL), dried over anhydrous Na2SO4, filtered and concentrated in vacuo to give the title compound (110 mg, crude) as a yellow solid that required no further purification. LCMS (ESI) m/z: 578 [M+H]+.
    • Step 2:
  • Figure US20200115354A1-20200416-C00129
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • A mixture of 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (110 mg, 0.19 mmol) and formic acid (8.0 mL) was stirred at room temperature for 6 h. The reaction mixture was concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 0-30%/0.225% formic acid in water) to give the title compound (30 mg, 37%) as as a white solid. LCMS (ESI) m/z: 428 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.36 (s, 1H), 8.03 (d, J=6.0 Hz, 1H), 7.63 (d, J=7.2 Hz, 1H), 6.78 (d, J=13.2 Hz, 1H), 6.66 (d, J=6.0 Hz, 1H), 5.83 (d, J=7.6 Hz, 1H), 3.60-3.40 (m, 1H), 3.17-3.03 (m, 1H), 2.44 (s, 6H), 2.06-1.89 (m, 2H), 1.82-1.73 (m, 1H), 1.66-1.56 (m, 1H), 1.43-1.10 (m, 4H).
    • Example 12
  • Figure US20200115354A1-20200416-C00130
    • Step 1:
  • Figure US20200115354A1-20200416-C00131
    • 4-(((1S,2S)-2-aminocyclopentyl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • A mixture of 5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2,4-difluoro-N-thiazol-2-yl-benzenesulfonamide (165 mg, 0.36 mmol), (1S,2S)-cyclopentane-1,2-diamine; dihydrochloride (158 mg, 0.91 mmol) and potassium carbonate (297 mg, 2.15 mmol) in 1-methyl-2-pyrrolidinone (4 mL) was heated in a sealed vial at 60° C. for 18 h. The mixture was The mixture was mixed with 5% aq. citric acid and extracted with ethyl acetate. The organic extracts were washed with water and then discarded. All aqueous washings were combined, pH was adjusted to 10 by addition of 1 N aq NaOH and then extracted with ethyl acetate. The organic extracts were washed with water, brine, dried over Na2SO4 and concentrated to afford 154 mg (79.5%) of the title product, which was used in the next step without further purification. LCMS (ESI) m/z: 541 [M+H]+.
    • Step 2:
  • Figure US20200115354A1-20200416-C00132
    • 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclopentyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • 4-[[(1S,2S)-2-aminocyclopentyl]amino]-5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide (154 mg, 0.29 mmol) was dissolved in 4 ml of dichloroethane, 37% aqueous formaldehyde (0.064 mL, 0.86 mmol) and triethylamine (0.16 mL, 1.1 mmol) were added and the mixture was stirred for 10 min. sodium triacetoxyborohydride (217 mg, 1.02 mmol) was added in one portion and the mixture was stirred for 2 hours. The mixture was concentrated in vacuum, the residue partitioned between water and ethyl acetate. The organic extracts were washed with brine, dried over Na2SO4 and concentrated to afford 144 mg (89%) of the title compound, was used in the next step without further purification. LCMS (ESI) m/z: 569 [M+H]+.
    • Step 3:
  • Figure US20200115354A1-20200416-C00133
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclopentyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • 0.5 ml of trifluoroacetic acid was added to a solution of 5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-4-[[(1S,2S)-2-(dimethylamino)cyclopentyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide (144 mg, 0.25 mmol) in 2 ml of DCM. The mixture was stirred for 1 hour and concentrated in vacuum. The residue was triturated with ethyl ether forming a precipitate. The precipitate was filtered, washed with ethyl ether and dried on air to afford 99 mg (73%) of a crude title product as a trifluoroacetate salt. 15 mg of the product was purified by reverse phase liquid chromatography to afford 6.2 mg of the title product as a base. LCMS (ESI) m/z: 419 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.59 (d, J=7.3 Hz, 1H), 7.24 (d, J=4.6 Hz, 1H), 6.83-6.78 (m, 2H), 6.51 (s, 1H), 6.09 (br. s, 1H), 3.93-3.79 (m, 1H), 2.42 (s, 6H), 2.14-2.02 (m, 1H), 1.93-1.81 (m, 1H), 1.72-1.56 (m, 4H), 1.45-1.35 (m, 1H).
    • Example 13
  • Figure US20200115354A1-20200416-C00134
    • 4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2,5-difluoro-N-(1,2,4-thiadiazol-5-yl)benzenesulfonamide formate Step 1:
  • Figure US20200115354A1-20200416-C00135
    • N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2,5-difluoro-N-(1,2,4-thiadiazol-5-yl)benzenesulfonamide
  • Following the procedure described in Example 11, step 1 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide with N-(2,4-dimethoxybenzyl)-2,4,5-trifluoro-N-(1,2,4-thiadiazol-5-yl)benzenesulfonamide, N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2,5-difluoro-N-(1,2,4-thiadiazol-5-yl)benzenesulfonamide was obtained as an off-white solid. LCMS (ESI) m/z: 568.1 [M+H]+. 1H-NMR (300 MHz; CDCl3): δ 8.15 (s, 1H), 7.39-7.32 (m, 1H), 7.16-7.13 (m, 1H), 6.36-6.33 (m, 1H), 6.29 (d, J=2.2 Hz, 1H), 6.24-6.17 (m, 1H), 5.73-5.68 (m, 1H), 5.24 (s, 2H), 3.74 (d, J=2.0 Hz, 6H), 2.98-2.90 (m, 1H), 2.50-2.42 (m, 1H), 2.20-2.19 (m, 6H), 1.95-1.87 (m, 2H), 1.78-1.73 (m, 1H), 1.32-1.13 (m, 5H).
    • Step 2:
  • Figure US20200115354A1-20200416-C00136
    • 4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2,5-difluoro-N-(1,2,4-thiadiazol-5-yl)benzenesulfonamide formate
  • Following the procedure described in Example 11, step 2, 4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2,5-difluoro-N-(1,2,4-thiadiazol-5-yl)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 418.1 [M+H]+. 1H-NMR (300 MHz, d6-dmso) δ 10.03-9.13 (br s, 1H), 7.88 (s, 1H), 7.31 (dd, J=6.5, 11.3 Hz, 1H), 6.91 (dd, J=6.9, 12.4 Hz, 1H), 6.13 (d, J=10.1 Hz, 1H), 3.83-3.72 (m, 1H), 3.37-3.23 (m, 1H), 2.72-2.56 (m, 6H), 2.08-1.93 (m, 2H), 1.84-1.79 (m, 1H), 1.65-1.60 (m, 1H), 1.47-1.12 (m, 4H).
    • Example 14
  • Figure US20200115354A1-20200416-C00137
    • 5-bromo-4-[[(1S,2S)-2-(dimethylamino)cyclohexyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide
  • Figure US20200115354A1-20200416-C00138
    • Step 1:
  • Figure US20200115354A1-20200416-C00139
    • 5-bromo-N-[(2,4-dimethoxyphenyl)methyl]-2,4-difluoro-N-thiazol-2-yl-benzenesulfonamide
  • To a solution of N-[(2,4-dimethoxyphenyl)methyl]thiazol-2-amine (1.0 g, 4.0 mmol) in THF (10 mL) at −78° C. was added lithium bis(trimethylsilyl)amide (6.0 mL, 6.0 mmol, 1.0 M) dropwise under a nitrogen atmosphere. The reaction mixture was stirred at −78° C. for 0.5 h, then the cooling bath was removed and the reaction was warmed to room temperature for 0.5 h, then cooled back to −78° C. A solution of 5-bromo-2,4-difluorobenzenesulfonylchloride (1.8 g, 6.0 mmol) in THF (2 mL) at −78° C. was added dropwise to the mixture. The reaction mixture was stirred at room temperature for an additional 1 h. Sat. aq. NH4Cl (50 mL) was added and extracted with EtOAc (100 mL). The organic layer was dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (petroleum ether/EtOAc=6:1) to give the title compound (0.6 g, 30%) as a yellow solid. 1H NMR (400 MHz, CDCl3) δ 8.12-8.02 (m, 1H), 7.43 (d, J=3.2 Hz, 1H), 7.20 (d, J=8.4 Hz, 1H), 7.04 (d, J=3.6 Hz, 1H), 7.02-6.94 (m, 1H), 6.41-6.32 (m, 2H), 5.19 (s, 2H), 3.77 (s, 3H), 3.74 (s, 3H).
    • Step 2:
  • Figure US20200115354A1-20200416-C00140
    • 5-bromo-N-[(2,4-dimethoxyphenyl)methyl]-4-[[(1S,2S)-2-(dimethylamino)cyclohexyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide
  • To a solution of 5-bromo-N-[(2,4-dimethoxyphenyl)methyl]-2,4-difluoro-N-thiazol-2-yl-benzenesulfonamide (300 mg, 0.59 mmol) in DMF (3 mL) was added potassium carbonate (246 mg, 1.78 mmol) and (1S,2S)—N1,N1-dimethylcyclohexane-1,2-diamine (93 mg, 0.65 mmol). The reaction mixture was stirred at room temperature for 16 h. Water (10 mL) was added and extracted with EtOAc (10 mL×2). The combined organic layers were washed with water (10 mL×3), brine (15 mL), dried over anhydrous Na2SO4, filtered and concentrated in vacuo to give the title compound (187 mg, crude) as a yellow solid that required no further purification. LCMS (ESI) m/z: 627 [M+H]+.
    • Step 3:
  • Figure US20200115354A1-20200416-C00141
    • 5-bromo-4-[[(1S,2S)-2-(dimethylamino)cyclohexyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide
  • A mixture of 5-bromo-N-[(2,4-dimethoxyphenyl)methyl]-4-[[(1S,2S)-2-(dimethylamino)cyclohexyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide (187 mg, 0.30 mmol) and formic acid (2 mL) was stirred at room temperature for 2 h. The mixture was concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 26-56%/0.225% formic acid in water) to give the title compound (91 mg, 64%) as a white solid. LCMS (ESI) m/z: 477 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.16 (s, 1H), 7.74 (d, J=7.6 Hz, 1H), 7.19 (d, J=4.4 Hz, 1H), 6.75-6.70 (m, 2H), 5.78-5.73 (m, 1H), 3.42-3.34 (m, 1H), 2.82-2.74 (m, 1H), 2.27 (s, 6H), 2.13-2.05 (m, 1H), 1.93-1.85 (m, 1H), 1.79-1.74 (m, 1H), 1.66-1.57 (m, 1H), 1.44-1.05 (m, 4H).
    • Example 15
  • Figure US20200115354A1-20200416-C00142
    • 5-cyclopropyl-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00143
    • Step 1:
  • Figure US20200115354A1-20200416-C00144
    • 5-cyclopropyl-N-[(2,4-dimethoxyphenyl)methyl]-4-[[(1S,2S)-2-(dimethylamino)cyclohexyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide
  • To a solution of 5-bromo-N-[(2,4-dimethoxyphenyl)methyl]-4-[[(1S,2S)-2-(dimethylamino)cyclohexyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide (100 mg, 0.16 mmol) in toluene (2.5 mL) and water (0.5 mL) was added palladium(II) acetate (7.2 mg, 0.03 mmol), tricyclohexylphosphine (18 mg, 0.06 mmol), cyclopropylboronic acid (27 mg, 0.32 mmol) and K3PO4 (101 mg, 0.48 mmol). The reaction mixture was heated to 100° C. for 16 h. After cooling to room temperature, the mixture was concentrated in vacuo. EtOAc (30 mL) was added, washed with water (10 mL×3), brine (10 mL), dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (solvent gradient: 0-100% EtOAc in petroleum ether) to give the title compound (50 mg, 53%) as light yellow oil. LCMS (ESI) m/z: 589 [M+H]+.
    • Step 2:
  • Figure US20200115354A1-20200416-C00145
    • 5-cyclopropyl-4-[[(1S,2S)-2-(dimethylamino)cyclohexyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide
  • A mixture of 5-cyclopropyl-N-[(2,4-dimethoxyphenyl)methyl]-4-[[(1S,2S)-2-(dimethylamino)cyclohexyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide (50 mg, 0.08 mmol) and formic acid (1.5 mL) was stirred at room temperature for 2 h. The mixture was concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 18-48%/0.225% formic acid in water) to give the title compound (10 mg, 27%) as a light yellow solid. LCMS (ESI) m/z: 439 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.17 (s, 1H), 7.31 (d, J=8.0 Hz, 1H), 7.18 (d, J=4.4 Hz, 1H), 6.72 (d, J=4.4 Hz, 1H), 6.46 (d, J=13.2 Hz, 1H), 5.96 (s, 1H), 3.24-3.13 (m, 1H), 2.70-2.60 (m, 1H), 2.23 (s, 6H), 2.22-2.12 (m, 1H), 1.91-1.74 (m, 2H), 1.68-1.48 (m, 2H), 1.45-1.08 (m, 4H), 0.95-0.83 (m, 2H), 0.60-0.50 (m, 1H), 0.32-0.22 (m, 1H).
    • Example 16
  • Figure US20200115354A1-20200416-C00146
    • 5-chloro-4-[[(1S,2S)-2-(cyclobutylamino)cyclohexyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide
  • Following the procedure described in Example 2 and making non-critical variations as required to replace formaldehyde with cyclobutanone, 5-chloro-4-[[(1S,2S)-2-(cyclobutylamino)cyclohexyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 459.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.50 (s, 1H), 7.54 (d, J=7.3 Hz, 1H), 6.92 (d, J=3.8 Hz, 1H), 6.54 (d, J=12.6 Hz, 1H), 6.43 (d, J=3.8 Hz, 1H), 5.50-5.30 (m, 1H), 2.11-2.01 (m, 2H), 1.92 (dd, J=32.5, 12.0 Hz, 3H), 1.77 (s, 3H), 1.58-1.45 (m, 7H), 1.23 (s, 3H), 1.04 (d, J=6.1 Hz, 2H), 0.94 (d, J=6.5 Hz, 2H).
    • Example 17
  • Figure US20200115354A1-20200416-C00147
    • 5-chloro-4-[[(1S,2S)-2-(diethylamino)cyclohexyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide
  • Following the procedure described in Example 2 and making non-critical variations as required to replace formaldehyde with acetaldehyde, 5-chloro-4-[[(1S,2S)-2-(diethylamino)cyclohexyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 461.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.55 (d, J=7.3 Hz, 1H), 6.92 (d, J=3.8 Hz, 1H), 6.51 (d, J=12.3 Hz, 1H), 6.43 (d, J=3.8 Hz, 1H), 6.27 (s, 3H), 5.78 (d, J=2.2 Hz, 1H), 3.06 (t, J=10.3 Hz, 1H), 2.50-2.37 (m, 2H), 2.34-2.19 (m, 2H), 1.85-1.74 (m, 1H), 1.59 (s, 1H), 1.40 (d, J=12.9 Hz, 1H), 1.24 (s, 1H), 1.17-1.05 (m, 1H), 0.98-0.86 (m, 6H).
    • Example 18
  • Figure US20200115354A1-20200416-C00148
    • 5-chloro-4-(((1S,2S)-2-(ethyl(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 2 and making non-critical variations as required to replace 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide and formaldehyde, 5-chloro-2-fluoro-4-[[(1S,2S)-2-(methylamino)cyclohexyl]amino]-N-thiazol-2-yl-benzenesulfonamide and acetaldehyde, 5-chloro-4-[[(1S,2S)-2-[ethyl(methyl)amino]cyclohexyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 447.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.58 (d, J=6.8 Hz, 1H), 7.18 (d, J=4.8 Hz, 1H), 6.75-6.65 (m, 2H), 5.88 (d, J=4.4 Hz, 1H), 2.78-2.68 (m, 1H), 2.43-2.38 (m, 2H), 2.16 (s, 3H), 2.13-2.05 (m, 1H), 1.87-1.70 (m, 2H), 1.66-1.55 (m, 1H), 1.43-1.05 (m, 5H), 0.96 (t, J=7.2 Hz, 3H).
    • Example 19
  • Figure US20200115354A1-20200416-C00149
    • 5-chloro-4-(((1S,2S)-2-(cyclopropyl(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 18 and making non-critical variations as required to replace acetaldehyde with (1-ethoxycyclopropoxy)trimethylsilane, 5-chloro-4-[[(1S,2S)-2-[cyclopropyl(methyl)amino]cyclohexyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 459.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.57 (d, J=7.6 Hz, 1H), 7.26 (d, J=4.8 Hz, 1H), 6.81 (d, J=4.8 Hz, 1H), 6.69 (d, J=12.8 Hz, 1H), 5.59 (d, J=3.6 Hz, 1H), 3.27-3.16 (m, 1H), 2.71-2.62 (m, 1H), 2.19-2.09 (m, 1H), 2.13 (s, 3H), 2.03-1.95 (m, 1H), 1.94-1.85 (m, 1H), 1.83-1.73 (m, 1H), 1.66-1.57 (m, 1H), 1.45-1.06 (m, 4H), 0.48-0.36 (m, 2H), 0.29-0.20 (m, 1H), 0.16-0.08 (m, 1H).
    • Example 20
  • Figure US20200115354A1-20200416-C00150
    • 5-chloro-2-fluoro-4-(methyl((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following a procedure similar to that of Example 3, 5-chloro-2-fluoro-4-(methyl((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide was obtained. LCMS (ESI) m/z: 433.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.67 (d, J=7.4 Hz, 1H), 7.11 (dd, J=11.8, 4.4 Hz, 1H), 7.05-6.96 (m, 1H), 6.58-6.51 (m, 1H), 3.29 (s, 3H), 3.13-3.05 (m, 1H), 2.68 (d, J=7.6 Hz, 2H), 2.50 (d, J=13.1 Hz, 3H), 2.18 (d, J=9.1 Hz, 1H), 1.69 (ddd, J=31.0, 24.2, 10.2 Hz, 2H), 1.58 (s, 1H), 1.51 (d, J=11.0 Hz, 1H), 1.22 (dd, J=13.7, 5.0 Hz, 2H).
    • Example 21
  • Figure US20200115354A1-20200416-C00151
    • 4-(((1S,2S)-2-(azetidin-1-yl)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following a procedure similar to that of Example 3, 4-(((1S,2S)-2-(azetidin-1-yl)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide was obtained. LCMS (ESI) m/z: 445.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.57 (d, J=7.3 Hz, 1H), 7.13 (d, J=4.3 Hz, 1H), 6.72 (d, J=13.2 Hz, 1H), 6.68 (d, J=4.3 Hz, 1H), 5.77 (d, J=9.2 Hz, 1H), 3.61-3.38 (m, 5H), 2.75 (d, J=11.9 Hz, 1H), 1.98 (p, J=7.2 Hz, 2H), 1.87 (d, J=12.1 Hz, 1H), 1.79-1.63 (m, 2H), 1.59 (d, J=12.0 Hz, 1H), 1.34 (q, J=11.2 Hz, 2H), 1.24-1.02 (m, 2H).
    • Example 22
  • Figure US20200115354A1-20200416-C00152
    • 5-chloro-4-(((1S,2S)-2-(ethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following a procedure similar to Example 3, 5-chloro-4-(((1S,2S)-2-(ethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide was obtained. LCMS (ESI) m/z: 428.1 [M+H]+. H NMR (400 MHz, DMSO-d6) δ 8.28 (s, 1H), 7.93 (d, J=6.0 Hz, 1H), 7.61 (d, J=7.2 Hz, 1H), 6.72 (d, J=12.7 Hz, 1H), 6.56 (d, J=5.9 Hz, 1H), 5.74 (d, J=9.5 Hz, 1H), 3.46 (s, 2H), 3.10-2.83 (m, 2H), 2.18-2.07 (m, 1H), 1.95-1.81 (m, 1H), 1.80-1.70 (m, 1H), 1.68-1.57 (m, 1H), 1.44-1.19 (m, 4H), 1.16 (t, J=7.1 Hz, 3H).
    • Example 23
  • Figure US20200115354A1-20200416-C00153
    • 5-chloro-4-(((1S,6S)-6-(dimethylamino)-4-(trifluoromethyl)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00154
    Figure US20200115354A1-20200416-C00155
    • Step 1:
  • Figure US20200115354A1-20200416-C00156
    • (S)-2-((tert-butoxycarbonyl)amino)pent-4-enoic Acid
  • To a solution of (2S)-2-aminopent-4-enoic acid (40.0 g, 347.43 mmol) in 1,4-dioxane (400 mL) at 0° C. was added NaOH (31.96 g, 799.1 mmol) in water (800 mL) and di-tert-butyl dicarbonate (91 g, 416.92 mmol) slowly. The reaction mixture was stirred at room temperature for 16 h. The reaction was concentrated in vacuo to get rid of dioxane. The aqueous phase was washed with EtOAc (600 mL×3). The aqueous phase was acidified to pH 2 with 2 M aq. H2SO4 and extracted with EtOAc (600 mL×3). The combined organic layers were washed with brine (600 mL), dried over anhydrous MgSO4, filtered and concentrated in vacuo to give the title compound (63.6 g, crude) as colorless oil that required no further purification. 1H NMR (400 MHz, DMSO-d6) δ 12.40 (s, 1H), 7.04 (d, J=8.0 Hz, 1H), 5.83-5.68 (m, 1H), 5.14-4.96 (m, 2H), 3.97-3.87 (m, 1H), 2.48-2.23 (m, 2H), 1.37 (s, 9H).
    • Step 2:
  • Figure US20200115354A1-20200416-C00157
    • (S)-tert-butyl(1-diazo-2-oxohex-5-en-3-yl)carbamate
  • To a solution of (S)-2-((tert-butoxycarbonyl)amino)pent-4-enoic acid (25.0 g, 116.14 mmol) in THF (500 mL) at 0° C. was added triethylamine (32.4 mL, 232.29 mmol) and methanesulfonyl chloride (26.61 g, 232.29 mmol). The mixture was stirred at 0° C. for 2 h and filtered. The filtrate was added (trimethylsilyl)diazomethane (174.22 mL, 348.43 mmol, 2 M in hexane) at 0° C. slowly. The mixture was stirred at 0° C. for an additional 2 h. The mixture was quenched with water (100 mL) slowly at 0° C. and extracted with EtOAc (250 mL×2). The combined organic layers were washed with brine (150 mL×2), dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (petroleum ether/EtOAc=10:1) to give the title compound (3.9 g, 14%) as light yellow oil. H NMR (400 MHz, CDCl3) δ 5.79-5.66 (m, 1H), 5.47 (s, 1H), 5.21-5.12 (m, 2H), 5.08 (s, 1H), 4.31-4.18 (m, 1H), 2.59-2.48 (m, 1H), 2.47-2.36 (m, 1H), 1.45 (s, 9H).
    • Step 3:
  • Figure US20200115354A1-20200416-C00158
    • (S)-3-((tert-butoxycarbonyl)amino)hex-5-enoic Acid
  • To a solution of (S)-tert-butyl(1-diazo-2-oxohex-5-en-3-yl)carbamate (15.3 g, 63.94 mmol) in 1,4-dioxane (180 mL) and water (20 mL) was added silver(I) oxide (1.48 g, 6.39 mmol). The mixture was sonicated in an ultrasound bath at room temperature for 1 h. The mixture was concentrated in vacuo. Water (200 mL) was added and the mixture was basified with solid NaHCO3 to pH 8 and then extracted with EtOAc (100 mL×2). The aqueous layer was acidified with 4 M aq. HCl to pH 2 and extracted with EtOAc (100 mL×3). The combined organic layers were washed with brine (50 mL), dried over anhydrous Na2SO4, filtered and concentrated in vacuo to give the title compound (9.1 g, crude) as light yellow oil that required no further purification. 1H NMR (400 MHz, CDCl3) δ 5.85-5.65 (m, 1H), 5.18-5.06 (m, 2H), 5.05-4.86 (m, 1H), 4.09-3.85 (m, 1H), 2.65-2.50 (m, 2H), 2.39-2.28 (m, 2H), 1.45 (s, 9H).
    • Step 4:
  • Figure US20200115354A1-20200416-C00159
    • (S)-tert-butyl 4-allyl-6-oxo-1,3-oxazinane-3-carboxylate
  • To a solution of (S)-3-((tert-butoxycarbonyl)amino)hex-5-enoic acid (9.1 g, 39.69 mmol) in toluene (80 mL) was added formaldehyde (5.96 g, 198.46 mmol) and (1S)-(+)-10-camphorsulfonic acid (1.84 g, 7.94 mmol) and 4A molecular sieve (13 g). The mixture was heated to 90° C. for 6 h under nitrogen atmosphere. After cooling to room temperature, the mixture was filtered and concentrated in vacuo. EtOAc (100 mL) was added and washed with sat. aq. NaHCO3 (50 mL) and brine (50 mL), dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (petroleum ether/EtOAc=5:1) to give the title compound (6.9 g, 72%) as light yellow oil. 1H NMR (400 MHz, CDCl3) δ 5.95-5.65 (m, 2H), 5.24-5.09 (m, 2H), 4.93 (d, J=10.8 Hz, 1H), 4.30-4.08 (m, 1H), 2.83-2.70 (m, 1H), 2.62-2.53 (m, 1H), 2.49-2.34 (m, 2H), 1.50 (s, 9H).
    • Step 5:
  • Figure US20200115354A1-20200416-C00160
    • (4S,5S)-tert-butyl 4-allyl-6-oxo-5-(2-(trifluoromethyl)allyl)-1,3-oxazinane-3-carboxylate
  • To a solution of (S)-tert-butyl 4-allyl-6-oxo-1,3-oxazinane-3-carboxylate (7.3 g, 30.26 mmol) and 2-(trifluoromethyl)allyl 4-methylbenzenesulfonate (9.33 g, 33.28 mmol) in THF (70 mL) −78° C. was added potassium bis(trimethylsilyl)amide (80 mL, 40 mmol, 0.5 M in toluene) slowly. The mixture was stirred at −78° C. for 3 h. The reaction was quenched with sat. aq. NH4Cl (20 mL) slowly. The organic layer was dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (petroleum ether/EtOAc=10:1) to give the title compound (4.3 g, 41%) as light yellow oil. 1H NMR (400 MHz, CDCl3) δ 5.86 (s, 1H), 5.85-5.71 (m, 2H), 5.67 (s, 1H), 5.25-5.14 (m, 2H), 5.03-4.87 (m, 1H), 4.14-3.81 (m, 1H), 2.93-2.76 (m, 2H), 2.74-2.60 (m, 1H), 2.45-2.28 (m, 2H), 1.49 (s, 9H).
    • Step 6:
  • Figure US20200115354A1-20200416-C00161
    • (4aS,8aS)-tert-butyl 4-oxo-6-(trifluoromethyl)-2,4,4a,5,8,8a-hexahydro-1H-benzo[d][1,3]oxazine-1-carboxylate
  • To a solution of (4S,5S)-tert-butyl 4-allyl-6-oxo-5-(2-(trifluoromethyl)allyl)-1,3-oxazinane-3-carboxylate (4.3 g, 12.31 mmol) in toluene (180 mL) was added Hoveyda-Grubbs catalyst 2nd generation (771 mg, 1.23 mmol). The mixture was heated to 100° C. for 2 h under nitrogen atmosphere. After cooling to room temperature, the mixture was purified by silica gel chromatography (petroleum ether/EtOAc=6:1) to give the title compound (2.8 g, 71%) as a light yellow solid. 1H NMR (400 MHz, CDCl3) δ 6.33 (s, 1H), 6.10-5.70 (m, 1H), 5.07 (s, 1H), 4.00-3.70 (m, 1H), 3.05-2.88 (m, 1H), 2.87-2.78 (m, 1H), 2.77-2.66 (m, 1H), 2.61-2.46 (m, 1H), 2.31-2.12 (m, 1H), 1.50 (s, 9H).
    • Step 7:
  • Figure US20200115354A1-20200416-C00162
    • (1S,6S)-6-((tert-butoxycarbonyl)amino)-3-(trifluoromethyl)cyclohex-3-enecarboxylic acid
  • To a solution of (4aS,8aS)-tert-butyl 4-oxo-6-(trifluoromethyl)-2,4,4a,5,8,8a-hexahydro-1H-benzo[d][1,3]oxazine-1-carboxylate (2.8 g, 8.71 mmol) in THF (250 mL) at 0° C. was added 4 M aq. LiOH (54.47 mL, 217.87 mmol). The mixture was stirred at room temperature for 16 h and concentrated in vacuo. Water (20 mL) was added and washed with petroleum ether (15 mL). The aqueous layer was acidified with 4 M HCl to pH 3 and extracted with EtOAc (150 mL). The organic layer was washed with brine (10 mL), dried over anhydrous Na2SO4, filtered and concentrated in vacuo to give the title compound (2.6 g, crude) as light yellow oil that required no further purification. 1H NMR (400 MHz, CDCl3) δ 6.28 (s, 1H), 4.27-3.99 (m, 1H), 3.01-2.80 (m, 1H), 2.77-2.58 (m, 2H), 2.56-2.42 (m, 1H), 2.25-2.10 (m, 1H), 1.45 (s, 9H).
    • Step 8:
  • Figure US20200115354A1-20200416-C00163
    • tert-butyl((1S,6S)-6-isocyanato-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate
  • To a solution of (1S,6S)-6-((tert-butoxycarbonyl)amino)-3-(trifluoromethyl)cyclohex-3-enecarboxylic acid (2.6 g, 8.41 mmol) in toluene (25 mL) was added triethylamine (2.34 mL, 16.81 mmol) and diphenylphosphoryl azide (2.71 mL, 12.61 mmol). The mixture was heated to 80° C. for 3 h under nitrogen atmosphere. After cooling to room temperature, the mixture was purified by silica gel chromatography (petroleum ether/EtOAc=1:1) to give the title compound (2.0 g, 78%) as a white solid. 1H NMR (400 MHz, CDCl3) δ 6.39 (s, 1H), 5.51 (s, 1H), 3.68-3.54 (m, 1H), 3.44-3.33 (m, 1H), 3.16-3.01 (m, 1H), 2.71-2.58 (m, 1H), 2.44-2.23 (m, 2H), 1.55 (s, 9H).
    • Step 9:
  • Figure US20200115354A1-20200416-C00164
    • tert-butyl((1S,6S)-6-amino-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate
  • To a solution of tert-butyl((1S,6S)-6-isocyanato-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate (2.0 g, 6.53 mmol) in THF (30 mL) was added 4 μM aq. NaOH (9.79 mL, 39.18 mmol). The mixture was stirred at room temperature for 16 h. The mixture was concentrated in vacuo. EtOAc (50 mL) was added and washed with brine (10 mL), dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (petroleum ether/EtOAc=10:1, 0.5% triethylamine) to give the title compound (0.6 g, 33%) as an off-white solid. LCMS (ESI) m/z: 281.1 [M+H]+. H NMR (400 MHz, CDCl3) δ 6.25 (s, 1H), 4.68-4.52 (m, 1H), 3.60-3.45 (m, 1H), 2.96-2.86 (m, 1H), 2.80-2.65 (m, 1H), 2.63-2.52 (m, 1H), 2.13-1.98 (m, 2H), 1.47 (s, 9H).
    • Step 10:
  • Figure US20200115354A1-20200416-C00165
    • tert-butyl((1S,6S)-6-(dimethylamino)-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate
  • To a solution of tert-butyl((1S,6S)-6-amino-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate (300 mg, 1.07 mmol) in 1,2-dichloroethane (9 mL) was added paraformaldehyde (161 mg, 5.35 mmol) and sodium triacetoxyborohydride (1.13 g, 5.35 mmol). The mixture was stirred at room temperature for 16 h. The reaction was quenched with sat. aq. NaHCO3 and extracted with EtOAc (15 mL). The organic layer was washed with brine (3 mL×2), dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (petroleum ether/EtOAc=2:1) to give the title compound (0.15 g, 46%) as light yellow oil. 1H NMR (400 MHz, CDCl3) δ 6.23 (s, 1H), 5.57 (s, 1H), 3.52-3.38 (m, 1H), 3.20-3.06 (m, 1H), 2.78-2.60 (m, 1H), 2.36-2.13 (m, 2H), 2.28 (s, 6H), 2.05-1.93 (m, 1H), 1.47 (s, 9H).
    • Step 11:
  • Figure US20200115354A1-20200416-C00166
    • (1S,2S)—N1,N1-dimethyl-5-(trifluoromethyl)cyclohex-4-ene-1,2-diamine
  • To a solution of tert-butyl((1S,6S)-6-(dimethylamino)-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate (40 mg, 0.13 mmol) in DCM (1 mL) was added trifluoroacetic acid (0.29 mL, 3.89 mmol). The mixture was stirred at room temperature for 1 h. The mixture was concentrated in vacuo to give the title compound (27 mg, crude) as light yellow oil that required no further purification.
    • Step 12:
  • Figure US20200115354A1-20200416-C00167
    • 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,6S)-6-(dimethylamino)-4-(trifluoromethyl)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of (1S,2S)—N1,N1-dimethyl-5-(trifluoromethyl)cyclohex-4-ene-1,2-diamine (27 mg, 0.13 mmol) in DMF (1 mL) was added potassium carbonate (90 mg, 0.65 mmol) and 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide (120 mg, 0.26 mmol). The mixture was stirred at room temperature for 16 h. EtOAc (20 mL) was added and washed with water (5 mL×3), brined (5 mL), dried over anhydrous Na2SO4, filtered and concentrated in vacuo to give the title compound (150 mg, crude) as light yellow oil that required no further purification. LCMS (ESI) m/z: 649.2 [M+H]+.
    • Step 13:
  • Figure US20200115354A1-20200416-C00168
    • 5-chloro-4-[[(1S,6S)-6-(dimethylamino)-4-(trifluoromethyl)cyclohex-3-en-1-yl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide
  • A mixture of 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,6S)-6-(dimethylamino)-4-(trifluoromethyl)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (150 mg, 0.07 mmol) and formic acid (3 mL) was stirred at room temperature for 1 h. The mixture was concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 12-42%/0.225% formic acid in water) to give the title compound (17 mg, 15%) as a white solid. LCMS (ESI) m/z: 499.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.60 (d, J=7.2 Hz, 1H), 7.25 (d, J=4.4 Hz, 1H), 6.85 (d, J=12.8 Hz, 1H), 6.80 (d, J=4.4 Hz, 1H), 6.35 (s, 1H), 6.07 (d, J=5.2 Hz, 1H), 3.70-3.60 (m, 1H), 3.15-3.05 (m, 1H), 2.93-2.80 (m, 1H), 2.54-2.50 (m, 1H), 2.32-2.26 (m, 1H), 2.22 (s, 6H), 2.14-2.00 (m, 1H).
    • Example 24 & Example 25
  • Figure US20200115354A1-20200416-C00169
    • 5-cyclopropyl-4-[(1S,2S)-2-(dimethylamino)cyclohexoxy]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide & 5-cyclopropyl-4-[(1R,2R)-2-(dimethylamino)cyclohexoxy]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide
  • Following the procedure described in Example 15 and making non-critical variations as required to replace 5-bromo-N-[(2,4-dimethoxyphenyl)methyl]-4-[[(1S,2S)-2-(dimethylamino)cyclohexyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide with racemic (trans)-5-chloro-N-(2,4-dimethoxybenzyl)-4-((2-(dimethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide and racemic (trans)-5-chloro-N-(2,4-dimethoxybenzyl)-2-((2-(dimethylamino)cyclohexyl)oxy)-4-fluoro-N-(thiazol-2-yl)benzenesulfonamide, racemic (trans)-5-cyclopropyl-4-((2-(dimethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide was obtained as a white solid. Racemic (trans)-5-cyclopropyl-4-((2-(dimethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide was separated by using chiral SFC (Chiralpak C2 (250 mm*30 mm, 10 um), Supercritical CO2/EtOH+0.1% NH4OH=55/55; 80 mL/min) to give 5-cyclopropyl-4-[(1S,2S)-2-(dimethylamino)cyclohexoxy]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide (first peak) as a white solid and 5-cyclopropyl-4-[(1R,2R)-2-(dimethylamino)cyclohexoxy]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide (second peak) as a white solid. Absolute configuration was arbitrarily assigned to each enantiomer. Example 24: LCMS (ESI) m/z: 440 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.20 (d, J=4.0 Hz, 1H), 7.19 (s, 1H), 7.10 (d, J=12.8 Hz, 1H), 6.74 (d, J=4.8 Hz, 1H), 4.64-4.52 (m, 1H), 2.77-2.68 (m, 1H), 2.34 (s, 6H), 2.10-1.99 (m, 2H), 1.86-1.78 (m, 1H), 1.72-1.56 (m, 2H), 1.40-1.23 (m, 4H), 0.95-0.87 (m, 2H), 0.62-0.53 (m, 2H). Example 25: LCMS (ESI) m/z: 440 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.20 (d, J=2.8 Hz, 1H), 7.19 (s, 1H), 7.11 (d, J=12.4 Hz, 1H), 6.75 (d, J=4.4 Hz, 1H), 4.64-4.52 (m, 1H), 2.78-2.69 (m, 1H), 2.34 (s, 6H), 2.09-1.99 (m, 2H), 1.88-1.77 (m, 1H), 1.73-1.56 (m, 2H), 1.40-1.23 (m, 4H), 0.94-0.83 (m, 2H), 0.63-0.51 (m, 2H).
    • Example 26
  • Figure US20200115354A1-20200416-C00170
    • 5-chloro-N-(5-chlorothiazol-2-yl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluorobenzenesulfonamide
  • Following the procedure described in Example 11 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide with 5-chloro-N-(5-chlorothiazol-2-yl)-N-[(2,4-dimethoxyphenyl)methyl]-2,4-difluoro-benzenesulfonamide, 5-chloro-N-(5-chlorothiazol-2-yl)-4-[[(1S,2S)-2-(dimethylamino)cyclohexyl]amino]-2-fluorobenzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 466.9 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.55 (d, J=7.2 Hz, 1H), 6.97-6.88 (m, 2H), 5.89 (d, J=10.0 Hz, 1H), 3.90-3.37 (m, 1H), 3.20-3.10 (m, 1H), 2.62 (s, 6H), 2.08-2.01 (m, 1H), 1.98-1.87 (m, 1H), 1.86-1.76 (m, 1H), 1.66-1.57 (m, 1H), 1.49-1.31 (m, 2H), 1.29-1.16 (m, 2H).
    • Example 27
  • Figure US20200115354A1-20200416-C00171
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(4-methylthiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 11 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide with 5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2,4-difluoro-N-(4-methylthiazol-2-yl)benzenesulfonamide, 5-chloro-4-[[(1S,2S)-2-(dimethylamino)cyclohexyl]amino]-2-fluoro-N-(4-methylthiazol-2-yl)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 447.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.15 (s, 1H), 7.57 (d, J=7.2 Hz, 1H), 6.75 (d, J=13.2 Hz, 1H), 6.34 (s, 1H), 5.86 (d, J=6.0 Hz, 1H), 3.36-3.32 (m, 1H), 2.75-2.65 (m, 1H), 2.22 (s, 6H), 2.07 (s, 3H), 1.89-1.83 (m, 1H), 1.81-1.73 (m, 1H), 1.64-1.56 (m, 1H), 1.42-1.31 (m, 1H), 1.27-1.08 (m, 4H).
    • Example 28
  • Figure US20200115354A1-20200416-C00172
    • 4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-3-nitro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 11 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide with N-(2,4-dimethoxybenzyl)-4-fluoro-3-nitro-N-(thiazol-2-yl)benzenesulfonamide, the title compound was obtained as a white solid. LCMS (ESI) m/z: 426.1 [M+H]+.
    • Example 29
  • Figure US20200115354A1-20200416-C00173
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(methyl(propyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00174
    • Step 1:
  • Figure US20200115354A1-20200416-C00175
    • N-((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)-2,2,2-trifluoroacetamide
  • A mixture of 4-[[(1S,2S)-2-aminocyclohexyl]amino]-5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide (5.0 g, 9.01 mmol) and ethyl trifluoroacetate (1.18 mL, 9.91 mmol) in tetrahydrofuran (20 mL) was stirred at room temperature for 6h. The mixture was then concentrated and purified by silica chromatography (0 to 70% EtOAc/Heptane) to give the title compound (3.8 g, 65%) as yellow oil.
    • Step 2:
  • Figure US20200115354A1-20200416-C00176
    • 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Potassium tert-butoxide (390 mg, 3.5 mmol) was added to a solution of N-[(1S,2S)-2-[2-chloro-4-[(2,4-dimethoxyphenyl)methyl-thiazol-2-yl-sulfamoyl]-5-fluoro-anilino]cyclohexyl]-2,2,2-trifluoroacetamide (1.50 g, 2.30 mmol) in N,N-dimethylformamide (5 mL). After the mixture was stirred for 10 min, iodomethane (0.22 mL, 3.5 mmol) was added. The mixture was stirred for 1 h then cooled to 5° C. 20% aqueous NH4Cl was added. The resulting precipitate was collected by filtration and washed with water. The solid was dissolved in methanol (5 mL) and a solution of 10N KOH (3.8 mL) was added. The mixture was stirred at room temperature for 36h. The mixture was concentrated to remove methanol and the aqueous phase was extracted with DCM. The organic phase was dried with MgSO4, filtered and concentrated. The crude product was purified by silica gel chromatography (0 to 10% MeOH/DCM) to give title compound (1.30 g, 71%) as a yellow solid. LCMS (ESI) m/z: 569.1 [M+H]+.
    • Step 3:
  • Figure US20200115354A1-20200416-C00177
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(methyl(propyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • 1-Iodopropane (0.02 mL, 0.20 mmol) was added to a solution of 5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2-fluoro-4-[[(1S,2S)-2-(methylamino)cyclohexyl]amino]-N-thiazol-2-yl-benzenesulfonamide (75 mg, 0.13 mmol) and N,N-diisopropylethylamine (0.046 mL, 0.26 mmol) in N,N-dimethylformamide (2 mL) and the mixture was stirred at 60° C. for 16h. the reaction was cooled down and water was added. The resulting precipitate was collected by filtration and washed with water. The residue was stirred in formic acid (1 mL) for 30 min and concentrated. The crude residue was purified by reverse phase chromatography (acetonitrile 26-56%/0.225% formic acid in water) to give the title compound (23 mg, 29%) as a white solid.
  • LCMS (ESI) m/z: 461.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.41 (s, 1H), 7.58 (d, J=7.3 Hz, 1H), 7.22 (d, J=4.5 Hz, 1H), 6.80-6.67 (m, 2H), 5.86 (dd, J=4.7, 1.8 Hz, 1H), 3.29 (s, 1H), 2.63 (s, 1H), 2.50-2.28 (m, 2H), 2.12 (s, 3H), 2.07 (s, 1H), 1.79 (d, J=18.2 Hz, 2H), 1.61 (d, J=12.5 Hz, 1H), 1.37 (dtd, J=18.1, 13.7, 6.7 Hz, 3H), 1.32-1.06 (m, 3H), 0.77 (t, J=7.3 Hz, 3H).
    • Example 30
  • Figure US20200115354A1-20200416-C00178
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(pyrrolidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00179
  • Figure US20200115354A1-20200416-C00180
    • 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • A mixture of 5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2,4-difluoro-N-thiazol-2-yl-benzenesulfonamide (8.60 g, 18.7 mmol), (1S,2S)-cyclohexane-1,2-diamine (4.26 g, 37.3 mmol), and triethylamine (13.0 mL, 93.3 mmol) was stirred in N,N-dimethylformamide (93 mL) at room temperature for 2h. 100 mL of aqueous NaHCO3 were added and the aqueous phase was extracted with dichloromethane. The organic phase was dried with MgSO4, filtered and concentrated on silica gel. Purification by silica gel chromatography (0-10% MeOH/DCM gradient) to afford the title compound (8.70 g, 84%) as a white solid. LCMS (ESI) m/z: 556.1 [M+H]+. 1H NMR (400 MHz, Chloroform-d) δ 7.72 (d, J=7.1 Hz, 1H), 7.38 (d, J=3.6 Hz, 1H), 7.21 (dt, J=8.4, 1.0 Hz, 1H), 6.95 (d, J=3.6 Hz, 1H), 6.45 (d, J=12.7 Hz, 1H), 6.36 (d, J=7.4 Hz, 2H), 5.19 (s, 2H), 4.90-4.79 (m, 1H), 3.75 (s, 3H), 3.75 (s, 3H), 3.01-2.92 (m, 1H), 2.75-2.61 (m, 1H), 2.11-1.93 (m, 2H), 1.86-1.67 (m, 2H), 1.39-1.05 (m, 4H).
  • Figure US20200115354A1-20200416-C00181
    • 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(pyrrolidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • A mixture of 4-[[(1S,2S)-2-aminocyclohexyl]amino]-5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide (250 mg, 0.45 mmol), 1,4-dibromobutane (0.054 mL, 0.45 mmol), and N,N-diisopropylethylamine (0.16 mL, 0.9 mmol) was stirred in acetonitrile (1.8 mL) at reflux for 17h. The mixture was cooled down and diluted with DCM and aqueous NaHCO3. The phases were separated and the aqueous phase was extracted with DCM. The combined organic phases were dried with MgSO4, filtered and concentrated. The crude product was purified by silica gel chromatography to give the title compound (240 mg, 87.4%) as a white solid. LCMS (ESI) m/z: 609.1 [M+H]+. 1H NMR (400 MHz, Chloroform-d) δ 7.70 (d, J=7.1 Hz, 1H), 7.38 (d, J=3.6 Hz, 1H), 7.21 (dd, J=7.7, 0.9 Hz, 1H), 6.95 (d, J=3.6 Hz, 1H), 6.39-6.33 (m, 2H), 6.29 (d, J=12.7 Hz, 1H), 6.03 (s, 1H), 5.19 (s, 2H), 3.75 (d, J=1.1 Hz, 6H), 3.03 (ddd, J=13.2, 9.4, 3.6 Hz, 1H), 2.75-2.65 (m, 1H), 2.54 (tp, J=5.4, 2.5 Hz, 4H), 2.27 (d, J=12.6 Hz, 1H), 1.96-1.80 (m, 2H), 1.80-1.64 (m, 5H), 1.47-1.13 (m, 4H).
  • Figure US20200115354A1-20200416-C00182
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(pyrrolidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • A mixture of 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(pyrrolidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (240 mg, 0.39 mmol) and formic acid (2 mL) was stirred at room temperature for 2 h. The mixture was concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 26-56%/0.225% formic acid in water) to give the title compound (22 mg, 12%) as a white solid. LCMS (ESI) m/z: 459.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.59 (d, J=7.3 Hz, 1H), 7.16 (d, J=4.4 Hz, 1H), 6.80-6.66 (m, 2H), 5.86 (d, J=6.4 Hz, 1H), 3.41 (s, 1H), 2.99 (d, J=11.0 Hz, 1H), 2.78 (d, J=8.3 Hz, 2H), 2.69 (d, J=7.7 Hz, 2H), 2.07 (s, 2H), 1.90 (dd, J=12.6, 3.8 Hz, 1H), 1.81-1.73 (m, 1H), 1.76-1.56 (m, 5H), 1.47-1.14 (m, 4H).
    • Example 31
  • Figure US20200115354A1-20200416-C00183
    • 5-chloro-4-(((1S,2S)-2-((cyclopropylmethyl)(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 29 and making non-critical variations as required to replace the iodopropane with iodomethylcyclopropane, the title compound was obtained. LCMS (ESI) m/z: 473.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.38 (s, 1H), 7.60 (d, J=7.2 Hz, 1H), 7.21 (d, J=4.5 Hz, 1H), 6.79-6.66 (m, 2H), 5.93 (dd, J=4.6, 1.7 Hz, 1H), 2.74 (s, 1H), 2.31 (s, 2H), 2.21 (s, 4H), 2.07 (s, 1H), 1.79 (dd, J=20.7, 7.6 Hz, 2H), 1.61 (d, J=13.2 Hz, 1H), 1.36 (t, J=13.1 Hz, 1H), 1.30-1.06 (m, 3H), 0.82-0.72 (m, 1H), 0.50-0.33 (m, 2H), 0.13-0.03 (m, 1H).
    • Example 32
  • Figure US20200115354A1-20200416-C00184
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-((2-methoxyethyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide formate
  • Following the procedure described in Example 29 and making non-critical variations as required to replace the iodopropane with 2-bromoethyl methyl ether, the title compound was obtained as a white solid. LCMS (ESI) m/z: 477.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.15 (s, 1H), 7.59 (d, J=7.6 Hz, 1H), 7.24 (d, J=4.4 Hz, 1H), 6.79 (d, J=4.4 Hz, 1H), 6.69 (d, J=12.8 Hz, 1H), 5.92 (d, J=3.6 Hz, 1H), 3.34-3.21 (m, 3H), 3.13 (s, 3H), 2.66-2.57 (m, 2H), 2.52 (d, J=2.0 Hz, 1H), 2.17 (s, 3H), 2.16-2.08 (m, 1H), 1.87-1.71 (m, 2H), 1.64-1.54 (m, 1H), 1.42-1.31 (m, 1H), 1.27-1.21 (m, 2H), 1.17-1.05 (m, 1H).
    • Example 33
  • Figure US20200115354A1-20200416-C00185
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(isopropyl(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 18 and making non-critical variations as required to replace the acetaldehyde with acetone, the title compound was obtained. LCMS (ESI) m/z: 461.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.58 (d, J=7.3 Hz, 1H), 7.20 (d, J=4.5 Hz, 1H), 6.75 (d, J=4.4 Hz, 1H), 6.68 (d, J=12.7 Hz, 1H), 5.80 (d, J=4.2 Hz, 1H), 3.82-3.71 (m, 1H), 3.44-3.20 (m, 1H), 3.28 (s, 3H), 2.56-2.44 (m, 1H), 2.17 (d, J=6.1 Hz, 1H), 2.12 (s, 4H), 1.78 (dd, J=26.8, 11.8 Hz, 3H), 1.61 (d, J=12.8 Hz, 1H), 1.37 (d, J=12.5 Hz, 1H), 1.28 (d, J=33.2 Hz, 1H), 1.17 (d, J=12.4 Hz, 1H), 0.99 (d, J=6.3 Hz, 6H).
    • Example 34
  • Figure US20200115354A1-20200416-C00186
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(propylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 29, step 2 and making non-critical variations as required to replace the iodomethane with 1-iodopropane, the title compound was obtained. LCMS (ESI) m/z: 447.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.57 (d, J=7.4 Hz, 1H), 7.03 (d, J=4.1 Hz, 1H), 6.72 (d, J=12.8 Hz, 1H), 6.60-6.49 (m, 1H), 5.75 (d, J=9.3 Hz, 1H), 2.99 (d, J=10.6 Hz, 1H), 2.81-2.62 (m, 2H), 2.12-2.03 (m, 1H), 1.93 (dd, J=12.7, 3.7 Hz, 1H), 1.72 (d, J=11.7 Hz, 1H), 1.62 (d, J=12.9 Hz, 1H), 1.58-1.29 (m, 3H), 1.22 (dtd, J=16.5, 12.7, 6.4 Hz, 3H), 0.85 (t, J=7.4 Hz, 3H).
    • Example 35
  • Figure US20200115354A1-20200416-C00187
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide
  • Following the procedure described in Example 11 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(6-fluoropyridine-2-yl)benzenesulfonamide, 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 445.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.71 (dd, J=7.9, 5.7 Hz, 2H), 6.81-6.73 (m, 2H), 6.57-6.50 (m, 1H), 5.95 (d, J=6.4 Hz, 1H), 2.80 (s, 1H), 2.27 (s, 6H), 2.10-1.99 (m, 2H), 1.88 (d, J=9.1 Hz, 1H), 1.77 (d, J=11.0 Hz, 1H), 1.65-1.56 (m, 1H), 1.44-1.23 (m, 1H), 1.24-1.11 (m, 2H).
    • Example 36 & Example 82
  • Figure US20200115354A1-20200416-C00188
    • 5-chloro-4-(((1R,2R,4R)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00189
    • Step 1
  • Figure US20200115354A1-20200416-C00190
    • (trans)-tert-butyl(2-amino-4-(trifluoromethyl)cyclohexyl)carbamate
  • To a solution of (trans)-tert-butyl(6-amino-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate (300 mg, 1.07 mmol) in EtOAc (8 mL) was added Pd(OH)2 (20%, 451 mg, 0.64 mmol). The mixture was stirred at room temperature for 2 h under hydrogen atmosphere (15 psi). The mixture was filtered and concentrated in vacuo to give the title compound (0.28 g, crude) as a light yellow solid that required no further purification.
    • Step 2
  • Figure US20200115354A1-20200416-C00191
    • (trans)-tert-butyl(2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)carbamate
  • To a solution of (trans)-tert-butyl(2-amino-4-(trifluoromethyl)cyclohexyl)carbamate (260 mg, 0.92 mmol) in MeOH (1 mL) was added AcOH (0.11 mL, 1.84 mmol) and paraformaldehyde (138 mg, 4.61 mmol). The mixture was stirred at room temperature for 10 min and sodium cyanoborohydride (289 mg, 4.61 mmol) was added. The mixture was stirred at room temperature for an additional 16 h. The reaction was quenched with sat. aq. NaHCO3 (10 mL) and extracted with DCM (20 mL×3). The combined organic layer were dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (solvent gradient: 0-100% EtOAc (0.5% TEA) in petroleum ether) to give the title compound (280 mg, 98%) as a light yellow solid.
    • Step 3
  • Figure US20200115354A1-20200416-C00192
    • (trans)-N1,N1-dimethyl-5-(trifluoromethyl)cyclohexane-1,2-diamine
  • To a solution of (trans)-tert-butyl(2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)carbamate (260 mg, 0.84 mmol) in DCM (3 mL) was added trifluoroacetic acid (1.24 mL, 16.75 mmol). The mixture was stirred at room temperature for 2 h and concentrated in vacuo to give the title compound (176 mg, crude) as light yellow oil that required no further purification.
    • Step 4
  • Figure US20200115354A1-20200416-C00193
    • (trans, cis)-5-chloro-N-(2,4-dimethoxybenzyl)-4-((2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & (trans, trans)-5-chloro-N-(2,4-dimethoxybenzyl)-4-((2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide (1.16 g, 2.51 mmol) and N,N-diisopropylethylamine (0.69 mL, 4.19 mmol) in DMF (5 mL) was added (trans)-N1,N1-dimethyl-5-(trifluoromethyl)cyclohexane-1,2-diamine (176 mg, 0.84 mmol). The reaction mixture was stirred at room temperature for 16 h and concentrated in vacuo. The crude residue was purified by silica gel chromatography (solvent gradient: 0-10%-25% EtOAc (1% TEA) in petroleum ether) to give (trans, cis)-5-chloro-N-(2,4-dimethoxybenzyl)-4-((2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (160 mg, less polar on TLC) as a light yellow solid and (trans, trans)-5-chloro-N-(2,4-dimethoxybenzyl)-4-((2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (70 mg, more polar on TLC) as a light yellow solid. Less polar on TLC: LCMS (ESI) m/z: 651.3 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 7.74 (d, J=7.2 Hz, 1H), 7.40 (d, J=3.6 Hz, 1H), 7.22 (d, J=8.4 Hz, 1H), 6.97 (d, J=3.2 Hz, 1H), 6.41-6.33 (m, 2H), 6.28 (d, J=12.4 Hz, 1H), 5.93 (s, 1H), 5.20 (s, 2H), 3.76 (s, 3H), 3.76 (s, 3H), 3.07-2.96 (m, 1H), 2.65-2.55 (m, 1H), 2.49-2.39 (m, 1H), 2.25 (s, 3H), 2.25 (s, 3H), 2.21-2.11 (m, 2H), 2.05-1.96 (m, 1H), 1.53-1.42 (m, 1H), 1.41-1.31 (m, 1H), 1.28-1.23 (m, 1H). More polar on TLC: LCMS (ESI) m/z: 651.3 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 7.74 (d, J=7.2 Hz, 1H), 7.40 (d, J=3.6 Hz, 1H), 7.22 (d, J=8.0 Hz, 1H), 6.97 (d, J=3.6 Hz, 1H), 6.42-6.33 (m, 2H), 6.28 (d, J=12.4 Hz, 1H), 5.48 (s, 1H), 5.20 (s, 2H), 3.76 (s, 3H), 3.76 (s, 3H), 3.48-3.38 (m, 1H), 2.62-2.51 (m, 2H), 2.29 (s, 3H), 2.29 (s, 3H), 2.20-2.10 (m, 1H), 1.92-1.76 (m, 4H), 1.68-1.55 (m, 1H).
    • Step 5
  • Figure US20200115354A1-20200416-C00194
    • (trans, cis)-5-chloro-4-((2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate
  • A mixture of (trans, cis)-5-chloro-N-(2,4-dimethoxybenzyl)-4-((2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (160 mg, 0.25 mmol) and formic acid (5.0 mL) was stirred at room temperature for 1 h. The reaction mixture was concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 10-40%/0.225% formic acid in water) to give the title compound (82 mg, 67%) as a white solid. LCMS (ESI) m/z: 501.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.14 (s, 1H), 7.58 (d, J=6.8 Hz, 1H), 7.23 (d, J=3.6 Hz, 1H), 6.87-6.74 (m, 2H), 5.81 (d, J=4.8 Hz, 1H), 3.45-3.38 (m, 1H), 2.88-2.77 (m, 1H), 2.45-2.35 (m, 1H), 2.20 (s, 3H), 2.20 (s, 3H), 2.20-2.09 (m, 1H), 2.03-1.94 (m, 1H), 1.84-1.73 (m, 1H), 1.59-1.44 (m, 1H), 1.37-1.18 (m, 2H).
    • Step 6
  • Figure US20200115354A1-20200416-C00195
    • 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1R,2R,4R)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • (trans, cis)-5-chloro-4-((2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate (82 mg) was separated by using chiral SFC (Chiralpak OJ (250 mm*30 mm, 5 um), Supercritical CO2/EtOH+0.1% NH4OH=75/25; 60 mL/min) to give 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (20 mg, first peak) as a white solid and 5-chloro-4-(((1R,2R,4R)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (32 mg, second peak) as a white solid. Absolute configuration was arbitrarily assigned to each enantiomer. Example 82: LCMS (ESI) m/z: 501.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.58 (d, J=6.8 Hz, 1H), 7.23 (d, J=3.6 Hz, 1H), 6.87-6.74 (m, 2H), 5.81 (d, J=4.8 Hz, 1H), 3.45-3.38 (m, 1H), 2.88-2.77 (m, 1H), 2.45-2.35 (m, 1H), 2.20 (s, 3H), 2.20 (s, 3H), 2.20-2.09 (m, 1H), 2.03-1.94 (m, 1H), 1.84-1.73 (m, 1H), 1.59-1.44 (m, 1H), 1.37-1.18 (m, 2H). Example 36: LCMS (ESI) m/z: 501.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.58 (d, J=6.8 Hz, 1H), 7.23 (d, J=3.6 Hz, 1H), 6.87-6.74 (m, 2H), 5.81 (d, J=4.8 Hz, 1H), 3.45-3.38 (m, 1H), 2.88-2.77 (m, 1H), 2.45-2.35 (m, 1H), 2.20 (s, 3H), 2.20 (s, 3H), 2.20-2.09 (m, 1H), 2.03-1.94 (m, 1H), 1.84-1.73 (m, 1H), 1.59-1.44 (m, 1H), 1.37-1.18 (m, 2H).
    • Example 77 & Example 81
  • Figure US20200115354A1-20200416-C00196
    • 5-chloro-4-(((1S,2S,4R)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1R,2R,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Step 1
  • Figure US20200115354A1-20200416-C00197
    • (trans, trans)-5-chloro-4-((2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 36, step 5, and making non-critical variations as required to replace (trans, cis)-5-chloro-N-(2,4-dimethoxybenzyl)-4-((2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with (trans, trans)-5-chloro-N-(2,4-dimethoxybenzyl)-4-((2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide, (trans, trans)-5-chloro-4-((2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 501.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.60 (d, J=7.2 Hz, 1H), 7.24 (d, J=4.4 Hz, 1H), 6.83-6.74 (m, 2H), 5.74 (d, J=6.0 Hz, 1H), 3.66-3.50 (m, 1H), 2.78-2.67 (m, 2H), 2.23 (s, 3H), 2.23 (s, 3H), 2.01-1.90 (m, 1H), 1.90-1.66 (m, 4H), 1.54-1.40 (m, 1H).
    • Step 2
  • Figure US20200115354A1-20200416-C00198
    • 5-chloro-4-(((1R,2R,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1S,2S,4R)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • (trans, trans)-5-chloro-4-((2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (45 mg) was separated by using chiral SFC (Chiralpak AS (250 mm*30 mm, 5 um), Supercritical CO2/EtOH+0.1% NH4OH=75/25; 60 mL/min) to give 5-chloro-4-(((1R,2R,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (12 mg, first peak) as a white solid and 5-chloro-4-(((1S,2S,4R)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (8 mg, second peak) as a white solid. Absolute configuration was arbitrarily assigned to each enantiomer. Example 81: LCMS (ESI) m/z: 501.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.60 (d, J=7.2 Hz, 1H), 7.24 (d, J=4.4 Hz, 1H), 6.83-6.74 (m, 2H), 5.74 (d, J=6.0 Hz, 1H), 3.66-3.50 (m, 1H), 2.78-2.67 (m, 2H), 2.23 (s, 3H), 2.23 (s, 3H), 2.01-1.90 (m, 1H), 1.90-1.66 (m, 4H), 1.54-1.40 (m, 1H). Example 77: LCMS (ESI) m/z: 501.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.60 (d, J=7.2 Hz, 1H), 7.24 (d, J=4.4 Hz, 1H), 6.83-6.74 (m, 2H), 5.74 (d, J=6.0 Hz, 1H), 3.66-3.50 (m, 1H), 2.78-2.67 (m, 2H), 2.23 (s, 3H), 2.23 (s, 3H), 2.01-1.90 (m, 1H), 1.90-1.66 (m, 4H), 1.54-1.40 (m, 1H).
    • Example 37
  • Figure US20200115354A1-20200416-C00199
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-((2-fluoroethyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide formate
  • Following the procedure described in Example 29 and making non-critical variations as required to replace iodopropane with 1-bromo-2-fluoroethane, 5-chloro-2-fluoro-4-(((1S,2S)-2-((2-fluoroethyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 465.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.14 (s, 1H), 7.58 (d, J=7.2 Hz, 1H), 7.25 (d, J=4.4 Hz, 1H), 6.81 (d, J=4.8 Hz, 1H), 6.70 (d, J=13.2 Hz, 1H), 5.89 (d, J=3.6 Hz, 1H), 4.47-4.40 (m, 1H), 4.34-4.28 (m, 1H), 3.31-3.23 (m, 2H), 2.72-2.61 (m, 2H), 2.18 (s, 3H), 2.16-2.08 (m, 1H), 1.85-1.69 (m, 2H), 1.65-1.55 (m, 1H), 1.44-1.30 (m, 1H), 1.30-1.19 (m, 2H), 1.19-1.07 (m, 1H).
    • Example 38
  • Figure US20200115354A1-20200416-C00200
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-((2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide formate
  • Following the procedure described in Example 29 and making non-critical variations as required to replace iodopropane with 2-bromoethanol, 5-chloro-2-fluoro-4-(((1S,2S)-2-((2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 463.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.15 (s, 1H), 7.58 (d, J 7.2 Hz, 1H), 7.23 (d, J=4.4 Hz, 1H), 6.79 (d, J=4.8 Hz, 1H), 6.73 (d, J=13.2 Hz, 1H), 5.97 (d, J=4.8 Hz, 1H), 3.49-3.38 (m, 2H), 3.37-3.29 (m, 1H), 2.80-2.71 (m, 1H), 2.65-2.52 (m, 2H), 2.23 (s, 3H), 2.14-2.06 (m, 1H), 1.90-1.71 (m, 2H), 1.66-1.55 (m, 1H), 1.44-1.31 (m, 1H), 1.30-1.19 (m, 2H), 1.19-1.06 (m, 1H).
    • Example 39
  • Figure US20200115354A1-20200416-C00201
    • 4-(((1S,2S)-2-(azetidin-3-yl(methyl)amino)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide dihydrochloride
  • Figure US20200115354A1-20200416-C00202
    • Step 1
  • Figure US20200115354A1-20200416-C00203
    • tert-butyl 3-(((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)amino)azetidine-1-carboxylate
  • To a solution of 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (5.5 g, 9.91 mmol) in DCM (100 mL) was added AcOH (0.057 mL, 0.99 mmol) and tert-butyl 3-oxoazetidine-1-carboxylate (1.7 g, 9.91 mmol). The mixture was stirred at room temperature for 1 h and sodium triacetoxyborohydride (6.3 g, 29.73 mmol) was added. The mixture was stirred at room temperature for an additional 12 h. The reaction was quenched with 1N NaOH (35 mL). The organic layer was dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (solvent gradient: 0-40% EtOAc in petroleum ether) to give the title compound (5.39 g, 77%) as a yellow solid. 1H NMR (400 MHz, CDCl3) δ 7.74 (d, J=7.2 Hz, 1H), 7.40 (d, J=3.6 Hz, 1H), 7.22 (d, J=8.0 Hz, 1H), 6.97 (d, J=4.0 Hz, 1H), 6.42 (d, J=12.4 Hz, 1H), 6.40-6.35 (m, 2H), 5.20 (s, 2H), 4.97-4.88 (m, 1H), 4.14-4.06 (m, 2H), 3.76 (s, 3H), 3.76 (s, 3H), 3.69-3.61 (m, 1H), 3.60-3.53 (m, 2H), 3.09-2.98 (m, 1H), 2.51-2.41 (m, 1H), 2.14-2.07 (m, 1H), 2.04-1.95 (m, 1H), 1.83-1.74 (m, 2H), 1.43 (s, 9H), 1.35-1.27 (m, 2H), 1.23-1.13 (m, 2H).
    • Step 2
  • Figure US20200115354A1-20200416-C00204
    • tert-butyl 3-(((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)(methyl)amino)azetidine-1-carboxylate
  • To a solution of tert-butyl 3-(((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)amino)azetidine-1-carboxylate (5.39 g, 7.59 mmol) in MeOH (90 mL) was added paraformaldehyde (1.14 g, 37.94 mmol). The mixture was stirred at room temperature for 1 h and sodium cyanoborohydride (0.95 g, 15.18 mmol) was added. The mixture was stirred at room temperature for an additional 12 h. The reaction was quenched with water (10 mL) and concentrated in vacuo. The crude residue was purified by silica gel chromatography (petroleum ether/EtOAc=3:1) to give the title compound (5.1 g, 93%) as a white solid. 1H NMR (400 MHz, CDCl3) δ 7.72 (d, J=6.4 Hz, 1H), 7.40 (dd, J=3.6, 1.2 Hz, 1H), 7.21 (d, J=8.0 Hz, 1H), 6.98 (dd, J=3.2, 1.2 Hz, 1H), 6.42-6.34 (m, 2H), 6.29 (d, J=12.4 Hz, 1H), 5.86 (s, 1H), 5.19 (s, 2H), 4.36 (s, 1H), 3.99-3.89 (m, 2H), 3.86-3.78 (m, 2H), 3.76 (s, 3H), 3.76 (s, 3H), 3.62-3.52 (m, 1H), 3.06-2.94 (m, 1H), 2.54-2.42 (m, 1H), 2.39-2.26 (m, 1H), 2.08 (s, 3H), 1.94-1.71 (m, 3H), 1.45 (s, 9H), 1.37-1.26 (m, 3H), 1.22-1.06 (m, 1H)
    • Step 3
  • Figure US20200115354A1-20200416-C00205
    • 4-(((1S,2S)-2-(azetidin-3-yl(methyl)amino)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide dihydrochloride
  • A mixture of tert-butyl 3-(((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)(methyl)amino)azetidine-1-carboxylate (1.1 g, 1.52 mmol) and formic acid (33 mL) was stirred at room temperature for 4 h. The mixture was concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 3-33%/0.05% HCl in water) to give the title compound (603 mg, 83%) as a white solid. LCMS (ESI) m/z: 474.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.91 (s, 1H), 11.85-9.95 (m, 1H), 9.81 (s, 1H), 9.60-8.96 (m, 1H), 7.63 (d, J=7.2 Hz, 1H), 7.27 (d, J=4.4 Hz, 1H), 7.08 (s, 1H), 6.85 (d, J=4.4 Hz, 1H), 6.32 (s, 1H), 4.66-3.86 (m, 5H), 3.68-3.55 (m, 1H), 2.63 (s, 3H), 2.53-2.51 (m, 1H), 2.14-1.69 (m, 3H), 1.67-1.53 (m, 1H), 1.52-1.31 (m, 3H), 1.30-1.15 (m, 1H).
    • Example 40
  • Figure US20200115354A1-20200416-C00206
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(5-methylthiazol-2-yl)benzenesulfonamide formate
  • Following the procedure described in Example 11 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(5-methylthiazol-2-yl)benzenesulfonamide, 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(5-methylthiazol-2-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 447.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.16 (s, 1H), 7.56 (d, J=7.2 Hz, 1H), 6.93 (s, 1H), 6.76 (d, J=12.8 Hz, 1H), 5.86 (d, J=5.2 Hz, 1H), 3.39-3.35 (m, 1H), 2.76-2.69 (m, 1H), 2.24 (s, 3H), 2.24 (s, 3H), 2.17 (s, 3H), 2.11-2.03 (m, 1H), 1.91-1.83 (m, 1H), 1.81-1.71 (m, 1H), 1.66-1.56 (m, 1H), 1.44-1.30 (m, 1H), 1.28-1.07 (m, 3H).
    • Example 41
  • Figure US20200115354A1-20200416-C00207
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(methyl(1-methylazetidin-3-yl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide formate
  • To a solution of 4-(((1S,2S)-2-(azetidin-3-yl(methyl)amino)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (185 mg, 0.39 mmol) in MeOH (2 mL) was added paraformaldehyde (14 mg, 0.47 mmol). The mixture was stirred at room temperature for 1 h and sodium cyanoborohydride (49 mg, 0.78 mmol) was added. The mixture was stirred at room temperature for an additional 12 h. The reaction was quenched with water (1 mL) and concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 26-56%/0.225% formic acid in water) to give the title compound (77 mg, 39%) as a white solid. LCMS (ESI) m/z: 488.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.22 (s, 1H), 7.58 (d, J=7.2 Hz, 1H), 7.14 (d, J=4.4 Hz, 1H), 6.72-6.66 (m, 2H), 5.72-5.66 (m, 1H), 3.85-3.73 (m, 2H), 3.67-3.57 (m, 1H), 3.47-3.37 (m, 1H), 3.35-3.25 (m, 2H), 2.53 (s, 3H), 2.53-2.52 (m, 1H), 2.03 (s, 3H), 2.08-1.97 (m, 1H), 1.75-1.68 (m, 1H), 1.67-1.54 (m, 2H), 1.40-1.11 (m, 4H).
    • Example 42 & Example 43
  • Figure US20200115354A1-20200416-C00208
    • 5-chloro-4-(((1S,2S)-2-(ethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1R,2R)-2-(ethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Examples 7 & 8, and making non-critical variations as required to replace racemic (trans)-2-(dimethylamino)cyclohexanol with racemic (trans)-2-(ethylamino)cyclohexan-1-ol, the title compounds was obtained as a mixture of enantiomers. The individual isomers were separated by chiral SFC and the stereochemistry was assigned arbitrarily.
  • Example 42: LCMS (ESI) m/z: 434 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.80 (d, J=7.3 Hz, 1H), 7.50 (d, J=11.8 Hz, 1H), 7.29 (d, J=4.6 Hz, 1H), 6.87 (d, J=4.5 Hz, 1H), 4.57 (td, J=10.0, 4.3 Hz, 1H), 3.50-3.36 (m, 1H), 3.13 (q, J=7.2 Hz, 2H), 2.16 (br.t, J=8.9, 5.9, 4.2 Hz, 2H), 1.78-1.60 (m, 2H), 1.51-1.25 (m, 4H), 1.20 (t, J=7.2 Hz, 3H)
  • Example 43: LCMS (ESI) m/z: 434 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.80 (d, J=7.4 Hz, 1H), 7.51 (d, J=11.8 Hz, 1H), 7.30 (d, J=4.6 Hz, 1H), 6.88 (d, J=4.5 Hz, 1H), 4.57 (td, J=10.0, 4.4 Hz, 1H), 3.47-3.33 (m, 1H), 3.13 (q, J=7.2 Hz, 2H), 2.16 (br. t, 2H), 1.80-1.60 (m, 2H), 1.52-1.25 (m, 4H), 1.21 (t, J=7.2 Hz, 3H).
  • Figure US20200115354A1-20200416-C00209
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(2-methylpyrrolidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 30 and making non-critical variations as required, the title compound was obtained as a mixture of diastereomers. LCMS (ESI) m/z: 473.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 11.83 (s, 1H), 7.58 (d, J=7.3 Hz, 1H), 7.19 (d, J=4.4 Hz, 1H), 6.77-6.69 (m, 2H), 5.96 (dd, J=6.4, 1.8 Hz, 1H), 3.17 (s, 1H), 3.00 (s, 1H), 2.95 (s, 1H), 2.75 (t, J=8.4 Hz, 1H), 2.10-1.99 (m, 1H), 1.88-1.64 (m, 3H), 1.59 (tt, J=8.5, 4.1 Hz, 1H), 1.48-1.33 (m, 1H), 1.32-1.11 (m, 2H), 0.89 (d, J=6.1 Hz, 3H).
    • Example 45
  • Figure US20200115354A1-20200416-C00210
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-((2-hydroxypropyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 29 and making non-critical variations as required to replace the iodopropane with 1-bromo-2-propanol, the title compound was obtained as a mixture of diastereomers. LCMS (ESI) m/z: 477.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.57 (d, J=7.3 Hz, 1H), 7.23 (d, J=4.5 Hz, 1H), 6.79 (d, J=4.6 Hz, 1H), 6.71 (d, J=12.9 Hz, 1H), 5.90 (d, J=10.1 Hz, 1H), 4.25 (s, 1H), 3.64 (s, 1H), 3.43-3.30 (m, 1H), 3.29 (s, 4H), 3.30-3.20 (m, 1H), 2.46 (s, 5H), 2.16 (d, J=13.7 Hz, 3H), 1.82 (s, 1H), 1.76 (s, 1H), 1.60 (d, J=13.5 Hz, 1H), 1.34 (dd, J=23.7, 11.5 Hz, 1H), 1.25 (d, J=11.1 Hz, 2H), 1.13 (d, J=17.1 Hz, 1H), 1.02 (d, J=6.2 Hz, 1H), 0.90 (d, J=6.1 Hz, 1H).
    • Example 46
  • Figure US20200115354A1-20200416-C00211
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-4-yl)benzenesulfonamide
  • Following the procedure described in Example 11 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide with tert-butyl((5-chloro-2,4-difluorophenyl)sulfonyl)(thiazol-4-yl)carbamate, 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-4-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 433.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 10.97 (s, 1H), 8.86 (d, J=2.1 Hz, 1H), 7.58 (d, J=7.4 Hz, 1H), 6.97-6.86 (m, 1H), 6.75 (d, J=13.2 Hz, 1H), 6.02-5.84 (m, 1H), 3.17 (d, J=5.0 Hz, 1H), 2.64-2.55 (m, 1H), 2.16 (s, 6H), 2.13-2.03 (m, 1H), 1.90-1.70 (m, 2H), 1.60 (d, J=13.1 Hz, 1H), 1.35 (dd, J=14.6, 11.0 Hz, 1H), 1.28-1.09 (m, 3H).
    • Example 47
  • Figure US20200115354A1-20200416-C00212
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(3-hydroxyazetidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 30 and making non-critical variations as required, the title compound was obtained. LCMS (ESI) m/z: 461.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.58 (dd, J=9.6, 7.4 Hz, 1H), 7.16 (d, J=4.4 Hz, 1H), 6.74-6.66 (m, 2H), 5.74 (d, J=9.1 Hz, 1H), 5.30 (s, 1H), 4.08 (q, J=6.0 Hz, 1H), 3.55 (s, 1H), 3.44 (s, 1H), 3.12 (s, 1H), 2.93 (s, 1H), 2.54 (s, 1H), 2.47 (s, 2H), 1.82 (d, J=12.5 Hz, 1H), 1.73 (s, 1H), 1.65 (d, J=13.9 Hz, 2H), 1.58 (s, 1H), 1.33 (t, J=9.2 Hz, 2H), 1.24-1.13 (m, 1H), 1.05 (t, J=11.9 Hz, 1H).
    • Example 48
  • Figure US20200115354A1-20200416-C00213
    • 5-chloro-4-(((1R,6R)-6-(dimethylamino)-3-(trifluoromethyl)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate Step 1
  • Figure US20200115354A1-20200416-C00214
    • 4-(((1R,6R)-6-amino-3-(trifluoromethyl)cyclohex-3-en-1-yl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 1 and making non-critical variations as required to replace tert-butyl N-[(1S,2S)-2-aminocyclohexyl]carbamate with tert-butyl((1R,6R)-6-amino-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate, 4-(((1R,6R)-6-amino-3-(trifluoromethyl)cyclohex-3-en-1-yl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide was obtained as a light yellow solid. LCMS (ESI) m/z: 470.8 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00215
    • 5-chloro-4-(((1R,6R)-6-(dimethylamino)-3-(trifluoromethyl)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 2 and making non-critical variations as required to replace 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 4-(((1R,6R)-6-amino-3-(trifluoromethyl)cyclohex-3-en-1-yl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide, 5-chloro-4-(((1R,6R)-6-(dimethylamino)-3-(trifluoromethyl)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 499.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.13 (s, 1H), 7.62 (d, J=7.2 Hz, 1H), 7.27 (d, J=4.4 Hz, 1H), 6.94 (d, J=13.2 Hz, 1H), 6.83 (d, J=4.4 Hz, 1H), 6.47 (s, 1H), 6.14 (d, J=6.4 Hz, 1H), 3.97-3.83 (m, 1H), 3.25-3.16 (m, 1H), 2.73-2.67 (m, 1H), 2.46-2.40 (m, 1H), 2.35-2.31 (m, 1H), 2.30 (s, 3H), 2.30 (s, 3H), 2.23-2.12 (m, 1H).
    • Example 49
  • Figure US20200115354A1-20200416-C00216
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(5-fluorothiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 11 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(5-fluorothiazol-2-yl)benzenesulfonamide, 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(5-fluorothiazol-2-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 451.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.55 (d, J=7.6 Hz, 1H), 6.92 (d, J=12.8 Hz, 1H), 6.71 (s, 1H), 5.86 (d, J=8.4 Hz, 1H), 3.88-3.70 (m, 1H), 3.43-3.35 (m, 1H), 2.60 (s, 3H), 2.60 (s, 3H), 2.10-1.89 (m, 2H), 1.87-1.77 (m, 1H), 1.69-1.58 (m, 1H), 1.47-1.30 (m, 2H), 1.29-1.13 (m, 2H).
    • Example 50
  • Figure US20200115354A1-20200416-C00217
    • 5-bromo-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide Formate Step 1:
  • Figure US20200115354A1-20200416-C00218
    • 5-bromo-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 29 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-bromo-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide, 5-bromo-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 613.2 [M+H]+.
    • Step 2:
  • Figure US20200115354A1-20200416-C00219
    • 5-bromo-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 11, step 2, 5-bromo-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 463.1 [M+H]+. H NMR (400 MHz, DMSO-d6) δ 8.19 (s, 1H), 7.73 (d, J=7.6 Hz, 1H), 7.06 (d, J=4.0 Hz, 1H), 6.75 (d, J=13.2 Hz, 1H), 6.60 (d, J=4.0 Hz, 1H), 5.63 (d, J=9.6 Hz, 1H), 3.64-3.60 (m, 1H), 3.23-3.15 (m, 1H), 2.52 (s, 3H), 2.15-2.06 (m, 1H), 1.92-1.84 (m, 1H), 1.79-1.70 (m, 1H), 1.66-1.58 (m, 1H), 1.44-1.17 (m, 4H).
    • Example 51
  • Figure US20200115354A1-20200416-C00220
    • 5-cyclopropyl-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 15 and making non-critical variations as required to replace 5-bromo-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-bromo-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide, 5-cyclopropyl-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 425.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.26 (s, 1H), 7.24 (d, J=8.0 Hz, 1H), 7.06 (d, J=4.4 Hz, 1H), 6.58 (d, J=4.0 Hz, 1H), 6.48 (d, J=13.6 Hz, 1H), 5.50 (d, J=9.6 Hz, 1H), 3.67-3.58 (m, 1H), 3.20-3.13 (m, 1H), 2.52 (s, 3H), 2.15-2.06 (m, 1H), 1.97-1.89 (m, 1H), 1.81-1.73 (m, 1H), 1.67-1.59 (m, 1H), 1.58-1.34 (m, 3H), 1.30-1.17 (m, 2H), 0.90-0.83 (m, 1H), 0.82-0.74 (m, 1H), 0.57-0.49 (m, 1H), 0.24-0.14 (m, 1H).
    • Example 52
  • Figure US20200115354A1-20200416-C00221
    • 5-bromo-4-(((1S,2S)-2-(ethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 50 and making non-critical variations as required to replace iodomethane with iodoethane, 5-bromo-4-(((1S,2S)-2-(ethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 477.0 [M+H]+. H NMR (400 MHz, DMSO-d6) δ 8.18 (s, 1H), 7.74 (d, J=7.6 Hz, 1H), 7.01 (d, J=4.0 Hz, 1H), 6.70 (d, J=12.8 Hz, 1H), 6.54 (d, J=4.0 Hz, 1H), 5.53 (d, J=8.0 Hz, 1H), 3.10-2.98 (m, 1H), 2.90-2.85 (m, 1H), 2.82-2.76 (m, 1H), 2.13-2.03 (m, 1H), 1.96-1.87 (m, 1H), 1.77-1.68 (m, 1H), 1.66-1.55 (m, 1H), 1.40-1.17 (m, 5H), 1.09 (t, J=7.2 Hz, 3H).
    • Example 53
  • Figure US20200115354A1-20200416-C00222
    • 4-(((1S,2S)-2-(azetidin-3-yl(methyl)amino)cyclohexyl)amino)-5-chloro-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide Step 1:
  • Figure US20200115354A1-20200416-C00223
    • 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 29, step 1 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide, 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide was obtained as a yellow solid. LCMS (ESI) m/z: 571.9 [M+Na]+.
    • Step 2:
  • Figure US20200115354A1-20200416-C00224
    • 4-(((1S,2S)-2-(azetidin-3-yl(methyl)amino)cyclohexyl)amino)-5-chloro-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 39 and making non-critical variations as required to replace 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide, 4-(((1S,2S)-2-(azetidin-3-yl(methyl)amino)cyclohexyl)amino)-5-chloro-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 469.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.31 (s, 1H), 7.95 (d, J=4.0 Hz, 1H), 7.58 (d, J=8.0 Hz, 1H), 7.63-6.58 (m, 2H), 5.51 (d, J=4.0 Hz, 1H), 3.95-3.80 (m, 5H), 3.34-3.31 (m, 1H), 2.67-2.58 (m, 1H), 2.14 (s, 3H), 2.00-1.97 (m, 1H), 1.71-1.56 (m, 3H), 1.33-1.12 (m, 4H).
    • Example 54
  • Figure US20200115354A1-20200416-C00225
    • 4-((1S,2S)-2-(dimethylamino)cyclohexyl)-N-(thiazol-2-yl)-3,4-dihydro-2H-benzo[b][1,4]oxazine-7-sulfonamide
  • Figure US20200115354A1-20200416-C00226
    Figure US20200115354A1-20200416-C00227
    • Step 1
  • Figure US20200115354A1-20200416-C00228
    • tert-butyl 2-(4-bromo-2-chlorophenoxy)acetate
  • tert-butyl 2-bromoacetate (3.10 mL, 21.0 mmol) was added to a mixture of 4-bromo-2-chlorophenol (4.15 g, 20.0 mmol) and cesium carbonate (7.17 g, 22.0 mmol) in N,N-dimethylformamide (10 mL). The mixture was heated at 80° C. for 18 hours, cooled down and mixed with water. The product was extracted with ethyl acetate. The organic extracts were washed with water (×2), brine, dried over Na2SO4 and concentrated to give tert-butyl 2-(4-bromo-2-chlorophenoxy)acetate (6.42 g, 99.8%) as a colorless oil, which was used without further purification in the next step.
    • Step 2
  • Figure US20200115354A1-20200416-C00229
    • 2-(4-bromo-2-chlorophenoxy)acetic Acid
  • 10 ml of TFA were added dropwise to a solution of tert-butyl 2-(4-bromo-2-chloro-phenoxy)acetate (6.42 g, 19.96 mmol) in 10 ml of DCM. The mixture was stirred for 18 hours at room temperature, then concentrated in vacuum and the residue partitioned between ethyl acetate and water. The organic extracts were washed with water (×2), brine, dried over MgSO4 and concentrated to give 2-(4-bromo-2-chlorophenoxy)acetic acid (5.13 g, 97%) as a colorless solid.
    • Step 3
  • Figure US20200115354A1-20200416-C00230
    • 2-(4-bromo-2-chlorophenoxy)-N-((1S,2S)-2-(dimethylamino)cyclohexyl)acetamide
  • HATU (4268 mg, 11.22 mmol) was added to a mixture of 2-(4-bromo-2-chlorophenoxy)acetic acid (2655 mg, 10.00 mmol), (1S,2S)—N1,N1-dimethylcyclohexane-1,2-diamine (1494 mg, 10.50 mmol) and triethylamine (3.50 mL, 25.1 mmol) in 30 ml of DMF. The mixture was stirred for 4 hours, concentrated in vacuum and the residue partitioned between water and ethyl acetate. The organic extracts were washed with sat. NaHCO3, water, brine, dried over Na2SO4 and concentrated to give 2-(4-bromo-2-chlorophenoxy)-N-((1S,2S)-2-(dimethylamino)cyclohexyl)acetamide (4010 mg, 100%) as a colorless oil, which was used without further purification in the next step. LCMS (ESI) m/z: 389.1 [M+H]+.
    • Step 4
  • Figure US20200115354A1-20200416-C00231
    • 7-bromo-4-((1S,2S)-2-(dimethylamino)cyclohexyl)-2H-benzo[b][1,4]oxazin-3(4H)-one
  • A mixture of 2-(4-bromo-2-chloro-phenoxy)-N-[(1S,2S)-2-(dimethylamino)cyclohexyl]acetamide (4010 mg, 10.29 mmol) and cesium carbonate (4023 mg, 12.35 mmol) in N,N-dimethylformamide (40 mL) was heated at 130° C. for 24 hours. The mixture was cooled, diluted with 30 ml of DCM, filtered and the filtrate concentrated in vacuum. The residue was dry loaded on a 80 g silica gel column and purified using 0-10% MeOH gradient in DCM to give 7-bromo-4-((1S,2S)-2-(dimethylamino)cyclohexyl)-2H-benzo[b][1,4]oxazin-3(4H)-one (1712 mg, 47%) as a yellowish oil. LCMS (ESI) m/z: 353.1 [M+H]+.
    • Step 5
  • Figure US20200115354A1-20200416-C00232
    • 7-(benzylthio)-4-((1S,2S)-2-(dimethylamino)cyclohexyl)-2H-benzo[b][1,4]oxazin-3(4H)-one
  • A degassed mixture of 7-bromo-4-[(1S,2S)-2-(dimethylamino)cyclohexyl]-1,4-benzoxazin-3-one (810 mg, 2.30 mmol), phenylmethanethiol (0.41 mL, 3.5 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (266 mg, 0.46 mmol), bis(dibenzylideneacetone)palladium (132 mg, 0.23 mmol) and triethylamine (1.0 mL, 7.1 mmol) in 1,4-dioxane (20 mL) was heated at 120° C. in a sealed vial for 3 hours. The mixture was diluted with water and extracted with ethyl acetate. The organic extracts were washed with water, brine, dried over Na2SO4 and concentrated. The residue was purified on a 24 g silica gel column eluting with 0-6% gradient of MeOH in DCM to give 7-(benzylthio)-4-((1S,2S)-2-(dimethylamino)cyclohexyl)-2H-benzo[b][1,4]oxazin-3(4H)-one (807 mg, 89%) as a yellowish oil. LCMS (ESI) m/z: 397.2 [M+H]+.
    • Step 6
  • Figure US20200115354A1-20200416-C00233
    • 4-((1S,2S)-2-(dimethylamino)cyclohexyl)-3-oxo-3,4-dihydro-2H-benzo[b][1,4]oxazine-7-sulfonyl Chloride
  • Sulfuryl chloride (0.33 mL, 4.1 mmol) was added dropwise to a mixture of 7-benzylsulfanyl-4-[(1S,2S)-2-(dimethylamino)cyclohexyl]-1,4-benzoxazin-3-one (400 mg, 1.00 mmol), acetic acid (0.23 mL, 4.0 mmol) and water (0.073 mL, 4.1 mmol) in dichloromethane (10 mL) at 0° C. The mixture was stirred at room temperature for 2 hours, concentrated in vacuum and the volatile materials were coevaporated with toluene twice to give 4-((1S,2S)-2-(dimethylamino)cyclohexyl)-3-oxo-3,4-dihydro-2H-benzo[b][1,4]oxazine-7-sulfonyl chloride (380 mg, 100%) as a yellowish oil. LCMS (ESI) m/z: 373.1 [M+H]+.
    • Step 7
  • Figure US20200115354A1-20200416-C00234
    • N-(2,4-dimethoxybenzyl)-4-((1S,2S)-2-(dimethylamino)cyclohexyl)-3-oxo-N-(thiazol-2-yl)-3,4-dihydro-2H-benzo[b][1,4]oxazine-7-sulfonamide
  • Lithium bis(trimethylsilyl)amide in THF (3.0 mL, 3.0 mmol, 1 mol/L) was added dropwise to a solution of N-[(2,4-dimethoxyphenyl)methyl]thiazol-2-amine (505 mg, 2.0 mmol) in THF (20 mL) at −78° C. The mixture was allowed to warm to 00C. After stirring for 30 minutes, the mixture was cooled down to −78° C. and a solution of 4-[(1S,2S)-2-(dimethylamino)cyclohexyl]-3-oxo-1,4-benzoxazine-7-sulfonyl chloride (G, 376 mg, 1.008 mmol) in THF (5 ml) The reaction was stirred at −78° C. for 30 min and at 0° C. for 2 hours, quenched with saturated aqueous ammonium chloride and extracted with ethyl acetate. The organic extracts were washed with water, brine, dried over sodium sulfate and concentrated. The crude product was purified by column chromatography (0-10% gradient of MeOH in DCM) to provide N-(2,4-dimethoxybenzyl)-4-((1S,2S)-2-(dimethylamino)cyclohexyl)-3-oxo-N-(thiazol-2-yl)-3,4-dihydro-2H-benzo[b][1,4]oxazine-7-sulfonamide (208 mg, 35% yield) as a yellowish solid.
  • LCMS (ESI) m/z: 587.1 [M+H]+.
    • Step 8
  • Figure US20200115354A1-20200416-C00235
    • N-(2,4-dimethoxybenzyl)-4-((1S,2S)-2-(dimethylamino)cyclohexyl)-N-(thiazol-2-yl)-3,4-dihydro-2H-benzo[b][1,4]oxazine-7-sulfonamide
  • 9-borabicyclo[3.3.1]nonane in THF (0.5 mol/L, 5.0 mL, 2.5 mmol) was added to a solution of N-(2,4-dimethoxybenzyl)-4-((1S,2S)-2-(dimethylamino)cyclohexyl)-3-oxo-N-(thiazol-2-yl)-3,4-dihydro-2H-benzo[b][1,4]oxazine-7-sulfonamide (120 mg, 0.21 mmol) in 2 ml of THF. The mixture was heated in a sealed vial at 65° C. for 12 hours. The mixture was quenched by stirring with 1 ml of water for 1 hour, concentrated and the residue purified on a 12 g silica gel column eluting with 0-8% MeOH gradient in DCM to give N-(2,4-dimethoxybenzyl)-4-((1S,2S)-2-(dimethylamino)cyclohexyl)-N-(thiazol-2-yl)-3,4-dihydro-2H-benzo[b][1,4]oxazine-7-sulfonamide (64 mg, 55%) as an off-white solid.
  • LCMS (ESI) m/z: 573.1 [M+H]+.
    • Step 9
  • Figure US20200115354A1-20200416-C00236
    • 4-((1S,2S)-2-(dimethylamino)cyclohexyl)-N-(thiazol-2-yl)-3,4-dihydro-2H-benzo[b][1,4]oxazine-7-sulfonamide
  • Trifluoroacetic acid (1 ml) was added dropwise to a solution of N-[(2,4-dimethoxyphenyl)methyl]-4-[[(1S,2S)-2-(dimethylamino)cyclohexyl]amino]-3-nitro-N-thiazol-2-yl-benzenesulfonamide (64 mg, 0.11 mmol) in 1 ml of DCM. The mixture was stirred for 2 hours, concentrated and the residue triturated with ethyl ether to form a precipitate. The precipitate was filtered, washed with ethyl ether and dried on air. The solid was dissolved in 10% aqueous acetonitrile, filtered and lyophilized to give 4-((1S,2S)-2-(dimethylamino)cyclohexyl)-N-(thiazol-2-yl)-3,4-dihydro-2H-benzo[b][1,4]oxazine-7-sulfonamide (29 mg, 61%) as a yellow light powder. LCMS (ESI) m/z: 423.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.20-7.14 (m, 2H), 6.98 (d, J=2.2 Hz, 1H), 6.89 (d, J=8.8 Hz, 1H), 6.71 (d, J=4.5 Hz, 1H), 4.21-4.08 (m, 3H), 3.80-3.68 (m, 1H), 3.41-3.35 (m, 1H), 3.40-3.21 (m, 2H), 2.69 (d, J=6.3 Hz, 1H), 2.15 (s, 6H), 1.86 (d, J=11.1 Hz, 1H), 1.75 (d, J=11.2 Hz, 1H), 1.66 (d, J=10.4 Hz, 2H), 1.39 (q, J=13.9, 13.2 Hz, 2H), 1.29-1.11 (m, 2H).
    • Example 55
  • Figure US20200115354A1-20200416-C00237
    • 4-(((1S,2S)-2-(azetidin-3-yl(methyl)amino)cyclohexyl)amino)-5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide
  • Following the procedure described in Example 39 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide with 5-chloro-I-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(6-fluoropyridine-2-yl)benzenesulfonamide, 4-(((1S,2S)-2-(azetidin-3-yl(methyl)amino)cyclohexyl)amino)-5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 486.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.18 (dd, J=2.6, 1.4 Hz, 1H), 7.62 (d, J=7.3 Hz, 1H), 7.54-7.36 (m, 1H), 6.72-6.51 (m, 2H), 6.29-6.09 (m, 1H), 5.67-5.47 (m, 1H), 3.98-3.71 (m, 5H), 2.67-2.54 (m, 1H), 2.13 (s, 3H), 2.03-1.95 (m, 1H), 1.77-1.53 (m, 3H), 1.40-1.07 (m, 5H).
    • Example 56
  • Figure US20200115354A1-20200416-C00238
    • 5-cyclopropyl-2-fluoro-4-(((1S,2S)-2-((2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide hydrochloride
  • Following the procedure described in Example 15 and making non-critical variations as required to replace 5-bromo-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-((2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide, 5-cyclopropyl-2-fluoro-4-(((1S,2S)-2-((2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide hydrochloride was obtained as a white solid. LCMS (ESI) m/z: 469.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.66 (s, 1H), 9.00-8.21 (m, 1H), 7.29 (d, J=8.0 Hz, 1H), 7.23 (d, J=4.8 Hz, 1H), 6.86-6.71 (m, 2H), 5.94-5.75 (m, 1H), 5.55-5.31 (m, 1H), 4.04-3.88 (m, 1H), 3.86-3.56 (m, 3H), 3.18-2.97 (m, 1H), 2.86-2.70 (m, 3H), 2.53-2.52 (m, 1H), 2.25-2.06 (m, 1H), 2.02-1.88 (m, 1H), 1.88-1.71 (m, 2H), 1.69-1.58 (m, 1H), 1.56-1.42 (m, 1H), 1.42-1.18 (m, 3H), 1.06-0.86 (m, 2H), 0.68-0.56 (m, 1H), 0.38-0.24 (m, 1H).
    • Example 57
  • Figure US20200115354A1-20200416-C00239
    • 4-(((1S,2S)-2-(azetidin-3-yl(methyl)amino)cyclohexyl)amino)-5-cyclopropyl-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide dihydrochloride
  • Following the procedure described in Example 15 and making non-critical variations as required to replace 5-bromo-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with tert-butyl 3-(((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)(methyl)amino)azetidine-1-carboxylate, 4-(((1S,2S)-2-(azetidin-3-yl(methyl)amino)cyclohexyl)amino)-5-cyclopropyl-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide dihydrochloride was obtained as a white solid. LCMS (ESI) m/z: 480.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.69 (s, 1H), 11.87-10.00 (m, 1H), 9.76 (s, 1H), 9.41-8.87 (m, 1H), 7.30 (d, J=8.0 Hz, 1H), 7.23 (d, J=4.4 Hz, 1H), 6.83-6.72 (m, 2H), 6.08-5.79 (m, 1H), 4.73-4.37 (m, 3H), 4.36-4.12 (m, 1H), 4.10-3.95 (m, 1H), 3.94-3.80 (m, 1H), 2.71-2.56 (m, 3H), 2.54-2.52 (m, 1H), 2.12-1.84 (m, 3H), 1.83-1.70 (m, 1H), 1.69-1.55 (m, 1H), 1.54-1.40 (m, 1H), 1.39-1.20 (m, 3H), 1.09-0.88 (m, 2H), 0.83-0.17 (m, 2H).
    • Example 58
  • Figure US20200115354A1-20200416-C00240
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(methyl(2-(methylamino)ethyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide formate
  • Figure US20200115354A1-20200416-C00241
    • Step 1
  • Figure US20200115354A1-20200416-C00242
    • tert-butyl(2-(((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)(methyl)amino)ethyl)(methyl)carbamate
  • To a solution of 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (298 mg, 0.52 mmol) in DCM (10 mL) was added AcOH (0.16 mL, 4.19 mmol), tert-butyl N-methyl-N-(2-oxoethyl)carbamate (91 mg, 0.52 mmol) and sodium triacetoxyborohydride (578 mg, 2.73 mmol). The mixture was stirred at room temperature for 1 h and washed with water (10 mL×3). The organic layer was dried over anhydrous Na2SO4, filtered and concentrated in vacuo to give the title compound (400 mg, crude) as light yellow oil that required no further purification. LCMS (ESI) m/z: 726.2 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 7.73 (d, J=7.2 Hz, 1H), 7.38 (d, J=3.6 Hz, 1H), 7.20 (d, J=9.2 Hz, 1H), 6.96 (d, J=3.6 Hz, 1H), 6.38-6.35 (m, 2H), 6.23-6.04 (m, 1H), 5.18 (s, 2H), 3.74 (s, 3H), 3.74 (s, 3H), 3.58-3.51 (m, 1H), 3.44-3.35 (m, 2H), 3.42-3.22 (m, 1H), 2.82 (s, 3H), 2.54-2.45 (m, 2H), 2.41-2.15 (m, 2H), 2.07 (s, 3H), 1.96-1.76 (m, 2H), 1.42 (s, 9H), 1.40-1.30 (m, 4H).
    • Step 2
  • Figure US20200115354A1-20200416-C00243
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(methyl(2-(methylamino)ethyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide Formate
  • A mixture of tert-butyl(2-(((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)(methyl)amino)ethyl)(methyl)carbamate (200 mg, 0.28 mmol) and formic acid (3 mL) was stirred at room temperature for 2 h. The mixture was concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 13-43%/0.225% formic acid in water) to give the title compound (54 mg, 41%) as a white solid. LCMS (ESI) m/z: 476.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.22 (s, 1H), 7.56 (d, J=7.2 Hz, 1H), 7.07 (d, J=4.4 Hz, 1H), 6.66 (d, J=13.2 Hz, 1H), 6.60 (d, J=4.4 Hz, 1H), 5.68 (d, J=6.0 Hz, 1H), 3.40-3.28 (m, 1H), 2.97-2.82 (m, 2H), 2.69-2.65 (m, 2H), 2.49 (s, 3H), 2.10 (s, 3H), 2.07-2.01 (m, 1H), 1.81-1.69 (m, 2H), 1.65-1.54 (m, 1H), 1.35-1.10 (m, 4H).
    • Example 59
  • Figure US20200115354A1-20200416-C00244
    • 5-chloro-4-(((3S,4S)-4-(dimethylamino)-3′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Step 1
  • Figure US20200115354A1-20200416-C00245
    • tert-butyl((3S,4S)-3-amino-3′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate
  • Following the procedure described in Example 23 and making non-critical variations as required to replace 2-(trifluoromethyl)allyl 4-methylbenzenesulfonate with 1-(3-iodoprop-1-en-2-yl)-3-(trifluoromethyl)benzene, tert-butyl((3S,4S)-3-amino-3′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate was obtained as a light yellow solid. 1H NMR (400 MHz, CDCl3) δ 7.62 (s, 1H), 7.57-7.49 (m, 2H), 7.48-7.41 (m, 1H), 6.10-6.07 (m, 1H), 4.71-4.60 (m, 1H), 3.65-3.59 (m, 1H), 3.05-2.95 (m, 1H), 2.87-2.71 (m, 2H), 2.45-2.36 (m, 1H), 2.26-2.07 (m, 1H), 1.48 (s, 9H).
    • Step 2
  • Figure US20200115354A1-20200416-C00246
    • 5-chloro-4-(((3S,4S)-4-(dimethylamino)-3′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 48 and making non-critical variations as required to replace tert-butyl((1R,6R)-6-amino-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate with tert-butyl ((3S,4S)-3-amino-3′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate, 5-chloro-4-(((3S,4S)-4-(dimethylamino)-3′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide was obtained as a light yellow solid. LCMS (ESI) m/z: 475.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.76-7.71 (m, 2H), 7.63-7.51 (m, 3H), 7.22 (d, J=4.4 Hz, 1H), 6.94 (d, J=12.4 Hz, 1H), 6.78 (d, J=4.4 Hz, 1H), 6.35-6.32 (m, 1H), 6.16-6.12 (m, 1H), 3.75-3.72 (m, 1H), 3.15-3.05 (m, 2H), 2.48-2.35 (m, 3H), 2.25 (s, 3H), 2.25 (s, 3H).
    • Example 60
  • Figure US20200115354A1-20200416-C00247
    • 5-chloro-2-fluoro-N-(pyrimidin-4-yl)-4-(((1S,2S)-2-(pyrrolidin-1-yl)cyclohexyl)amino)benzenesulfonamide
  • Following the procedure described in Example 30 and making non-critical variations as required to replace 5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2,4-difluoro-N-thiazol-2-yl-benzenesulfonamide with 4-[[(1S,2S)-2-aminocyclohexyl]amino]-5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2-fluoro-N-pyrimidin-4-yl-benzenesulfonamide, the title compound was obtained as a white solid. LCMS (ESI) m/z: 454.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.37 (s, 1H), 8.04 (d, J=6.1 Hz, 1H), 7.65 (d, J=7.3 Hz, 1H), 6.78 (d, J=12.8 Hz, 1H), 6.67 (d, J=6.0 Hz, 1H), 5.99-5.74 (m, 1H), 3.77-3.50 (m, 1H), 3.18-2.80 (m, 4H), 2.04-1.88 (m, 2H), 1.85-1.67 (m, 5H), 1.67-1.55 (m, 1H), 1.49-1.11 (m, 5H).
    • Example 61
  • Figure US20200115354A1-20200416-C00248
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(3-hydroxypyrrolidin-1-yl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 30 and making non-critical variations as required to replace 5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2,4-difluoro-N-thiazol-2-yl-benzenesulfonamide with 4-[[(1S,2S)-2-aminocyclohexyl]amino]-5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2-fluoro-N-pyrimidin-4-yl-benzenesulfonamide and 1,4-dibromobutane with 1,4-dibromobutan-2-ol, the title compound was obtained as a mixture of diastereomers. LCMS (ESI) m/z: 470.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.38 (s, 1H), 8.06 (d, J=6.1 Hz, 1H), 7.65 (d, J=7.4 Hz, 1H), 6.79-6.66 (m, 2H), 6.51 (s, 1H), 5.83 (d, J=7.6 Hz, 1H), 4.98 (s, 1H), 4.23 (s, 1H), 2.54 (s, 1H), 1.96 (s, 3H), 1.90 (dq, J=20.9, 7.3, 6.5 Hz, 1H), 1.78 (d, J=12.1 Hz, 1H), 1.63 (s, 2H), 1.60 (d, J=13.6 Hz, 1H), 1.42-1.36 (m, 1H), 1.40-1.16 (m, 4H).
    • Example 62
  • Figure US20200115354A1-20200416-C00249
    • 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-(3-hydroxypyrrolidin-1-yl)cyclohexyl)amino)benzenesulfonamide
  • Following the procedure described in Example 30 and making non-critical variations as required to replace 5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2,4-difluoro-N-thiazol-2-yl-benzenesulfonamide with 5-chloro-I-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(6-fluoropyridine-2-yl)benzenesulfonamide and 1,4-dibromobutane with 1,4-dibromobutan-2-ol, the title compound was obtained as a mixture of diastereomers. LCMS (ESI) m/z: 487.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 11.14 (s, 1H), 7.70 (d, J=7.4 Hz, 2H), 6.74 (dd, J=14.8, 10.3 Hz, 2H), 6.51 (s, 1H), 5.94 (d, J=5.9 Hz, 1H), 4.70 (s, 1H), 4.11 (d, J=20.6 Hz, 1H), 2.79 (s, 4H), 2.50-2.40 (m, 1H), 2.07 (s, 2H), 1.81 (ddt, J=35.8, 14.0, 6.1 Hz, 4H), 1.65-1.55 (m, 1H), 1.52 (d, J=9.0 Hz, 1H), 1.36 (ddd, J=15.5, 7.7, 3.1 Hz, 1H), 1.30 (s, 2H), 1.31-1.12 (m, 2H).
    • Example 63
  • Figure US20200115354A1-20200416-C00250
    • 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-(methyl(1-methylazetidin-3-yl)amino)cyclohexyl)amino)benzenesulfonamide
  • The mixture of 4-[[(1S,2S)-2-[azetidin-3-yl(methyl)amino]cyclohexyl]amino]-5-chloro-2-fluoro-N-(6-fluoro-2-pyridyl)benzenesulfonamide (Example 55, 100 mg, 0.206 mmol) and formaldehyde (9.27 mg, 0.309 mmol) in MeOH (1 mL) was stirred at room temperature for 30 min. Then NaCNBH3 (26 mg, 0.412 mmol) was added and the mixture was stirred for 18 h. The reaction was quenched with water (1 mL), and concentrated. The residue was purified by HPLC to give 5-chloro-2-fluoro-N-(6-fluoro-2-pyridyl)-4-[[(1S,2S)-2-[methyl-(1-methylazetidin-3-yl)amino]cyclohexyl]amino]benzenesulfonamide (31 mg, 29%) as a white solid. LCMS (ESI) m/z: 500.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.67 (d, J=7.4 Hz, 1H), 7.61 (q, J=8.3 Hz, 1H), 6.76-6.61 (m, 2H), 6.39 (dd, J=7.8, 2.7 Hz, 1H), 5.81-5.67 (m, 1H), 3.75-3.58 (m, 2H), 3.55-3.44 (m, 1H), 3.17-2.99 (m, 2H), 2.41 (s, 3H), 2.11-2.02 (m, 1H), 2.00 (s, 3H), 1.78-1.53 (m, 4H), 1.42-1.07 (m, 5H).
    • Example 64
  • Figure US20200115354A1-20200416-C00251
    • 5-cyclopropyl-4-(((1S,2S)-2-(ethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 15 and making non-critical variations as required to replace 5-bromo-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-bromo-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(ethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide, 5-cyclopropyl-4-(((1S,2S)-2-(ethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 439.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.22 (s, 1H), 7.29 (d, J=8.4 Hz, 1H), 7.09 (d, J=4.0 Hz, 1H), 6.63 (d, J=4.4 Hz, 1H), 6.54 (d, J=13.2 Hz, 1H), 5.57 (d, J=8.8 Hz, 1H), 3.30-3.25 (m, 1H), 3.20-3.10 (m, 1H), 3.01-2.77 (m, 2H), 2.20-2.15 (m, 1H), 2.0-1.9 (m, 1H), 1.8-1.7 (m, 1H), 1.67-1.53 (m, 2H), 1.47-1.32 (m, 2H), 1.30-1.19 (m, 2H), 1.14 (t, J=7.2 Hz, 3H), 0.99-0.78 (m, 2H), 0.60-0.40 (m, 1H), 0.35-0.25 (m, 1H).
    • Example 65
  • Figure US20200115354A1-20200416-C00252
    • 4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)-5-(trifluoromethyl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00253
    Figure US20200115354A1-20200416-C00254
    • Step 1
  • Figure US20200115354A1-20200416-C00255
    • benzyl(2,4-difluoro-5-(trifluoromethyl)phenyl)sulfane
  • To a solution of 1-bromo-2,4-difluoro-5-(trifluoromethyl)benzene (0.55 g, 1.81 mmol) in anhydrous 1,4-dioxane (5 mL) was added N,N-diisopropylethylamine (0.63 mL, 3.83 mmol), benzyl mercaptan (0.25 mL, 2.11 mmol), 4,5-bis(diphenylphosphino)-9,9-dimethylxanthene (0.11 g, 0.19 mmol) and tris(dibenzylideneacetone)dipalladium(0) (0.09 g, 0.10 mmol). The reaction mixture was heated to 120° C. for 2 h under a nitrogen atmosphere. After cooling to room temperature, the mixture was concentrated in vacuo. The crude residue was purified by silica gel chromatography (petroleum ether) to give the title compound (0.55 g, 94%) as colorless oil. 1H NMR (400 MHz, CDCl3) δ 7.51-7.43 (m, 1H), 7.32-7.18 (m, 5H), 6.99-6.92 (m, 1H), 4.07 (s, 2H).
    • Step 2
  • Figure US20200115354A1-20200416-C00256
    • 2,4-difluoro-5-(trifluoromethyl)benzene-1-sulfonyl chloride
  • To a solution of benzyl(2,4-difluoro-5-(trifluoromethyl)phenyl)sulfane (250 mg, 0.82 mmol) in THF (4 mL), AcOH (0.1 mL) and water (0.2 mL) was added 1,3-dichloro-5,5-dimethylhydantoin (486 mg, 2.46 mmol) slowly. The mixture was stirred at 0° C. for 3 h. EtOAc (50 mL) was added and washed with sat. aq. NaHCO3 (20 mL), brine (15 mL), dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (petroleum ether) to give the title compound (250 mg, 76%) as light yellow oil. 1H NMR (400 MHz, CDCl3) δ 8.34-8.29 (m, 1H), 7.33-7.24 (m, 1H).
    • Step 3
  • Figure US20200115354A1-20200416-C00257
    • N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)-5-(trifluoromethyl)benzenesulfonamide
  • To a solution of N-[(2,4-dimethoxyphenyl)methyl]pyrimidin-4-amine (0.16 g, 0.62 mmol) and 2,4-difluoro-5-(trifluoromethyl)benzene-1-sulfonyl chloride (0.25 g, 0.62 mmol) in THF (3 mL) at −78° C. was added lithium bis(trimethylsilyl)amide (0.69 mL, 0.69 mmol, 1.0 M in THF) dropwise under a nitrogen atmosphere. The reaction mixture was stirred at −78° C. for 2 h. Sat. aq. NH4Cl (2 mL) was added and extracted with EtOAc (10 mL). The organic layer was dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (solvent gradient: 0-20% EtOAc in petroleum ether) to give the title compound (100 mg, 32.4%) as a light yellow solid. 1H NMR (400 MHz, CDCl3) δ 8.16-8.10 (m, 1H), 7.44 (d, J=3.6 Hz, 1H), 7.20 (d, J=8.4 Hz, 1H), 7.10-7.01 (m, 2H), 6.41-6.31 (m, 2H), 5.19 (s, 2H), 3.77 (s, 3H), 3.73 (s, 3H).
    • Step 4
  • Figure US20200115354A1-20200416-C00258
    • N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)-5-(trifluoromethyl)benzenesulfonamide
  • To a solution of N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)-5-(trifluoromethyl)benzenesulfonamide (100 mg, 0.20 mmol) and N,N-diisopropylethylamine (0.17 mL, 1.01 mmol) in DMF (5 mL) was added (1S,2S)—N1,N1-dimethylcyclohexane-1,2-diamine (58 mg, 0.40 mmol). The reaction mixture was stirred at room temperature for 16 h. The mixture was diluted with EtOAc (30 mL), washed with water (20 mL), brine (20 mL), dried over anhydrous Na2SO4, filtered and concentrated in vacuo to give the title compound (100 mg, crude) as a light yellow solid that required no further purification. LCMS (ESI) m/z: 617.3 [M+H]+.
    • Step 5
  • Figure US20200115354A1-20200416-C00259
    • 4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)-5-(trifluoromethyl)benzenesulfonamide
  • A mixture of N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)-5-(trifluoromethyl)benzenesulfonamide (100 mg, 0.16 mmol) and formic acid (2.0 mL) was stirred at room temperature for 1 h. The reaction mixture was concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 10-40%/0.225% formic acid in water) to give the title compound (17 mg, 22%) as a white solid. LCMS (ESI) m/z: 467.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.80 (d, J=7.6 Hz, 1H), 7.22 (d, J=4.4 Hz, 1H), 6.84 (d, J=13.2 Hz, 1H), 6.77 (d, J=4.4 Hz, 1H), 6.01 (s, 1H), 3.33-3.28 (m, 1H), 2.65-2.58 (m, 1H), 2.21 (s, 3H), 2.21 (s, 3H), 2.18-2.11 (m, 1H), 1.88-1.74 (m, 2H), 1.66-1.56 (m, 1H), 1.45-1.30 (m, 1H), 1.28-1.18 (m, 2H), 1.17-1.05 (m, 1H).
    • Example 66
  • Figure US20200115354A1-20200416-C00260
    • 4-(((1S,2S)-2-(azetidin-3-yl(methyl)amino)cyclohexyl)oxy)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Figure US20200115354A1-20200416-C00261
    • Step 1
  • Figure US20200115354A1-20200416-C00262
    • tert-butyl 3-(((1S,2S)-2-hydroxycyclohexyl)amino)azetidine-1-carboxylate
  • To a solution of (1S,2S)-2-aminocyclohexanol (5.0 g, 43.41 mmol) in DCM (120 mL) was added AcOH (0.25 mL, 4.34 mmol) and tert-butyl 3-oxoazetidine-1-carboxylate (7.43 g, 43.41 mmol) and sodium triacetoxyborohydride (2.76 g, 130.24 mmol). The mixture was stirred at room temperature for 16 h and concentrated in vacuo. EtOAc (200 mL) was added and washed with water (100 mL×2), brine (50 mL). The organic layer was dried over anhydrous MgSO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (DCM/MeOH=20:1) to give the title compound (7.0 g, 60%) as colorless oil. 1H NMR (400 MHz, CDCl3) δ 3.96-3.86 (m, 2H), 3.85-3.76 (m, 2H), 3.65 (s, 1H), 3.61-3.52 (m, 1H), 3.40-3.30 (m, 1H), 2.15-2.10 (m, 1H), 1.82-1.58 (m, 4H), 1.43 (s, 9H), 1.31-1.08 (m, 4H).
    • Step 2
  • Figure US20200115354A1-20200416-C00263
    • tert-butyl 3-(((1S,2S)-2-hydroxycyclohexyl)(methyl)amino)azetidine-1-carboxylate
  • To a solution of tert-butyl 3-(((1S,2S)-2-hydroxycyclohexyl)amino)azetidine-1-carboxylate (6.0 g, 22.19 mmol) in MeOH (100 mL) was added paraformaldehyde (9.99 g, 110.96 mmol), AcOH (1.26 mL, 22.19 mmol) and sodium cyanoborohydride (2.8 g, 44.38 mmol). The mixture was stirred at room temperature for 16 h and concentrated in vacuo. EtOAc (100 mL) was added and washed with water (50 mL×2), brine (30 mL). The organic layer was dried over anhydrous MgSO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (DCM/MeOH=20:1) to give the title compound (4.5 g, 71%) as a white solid. 1H NMR (400 MHz, CDCl3) δ 3.96-3.86 (m, 2H), 3.85-3.77 (m, 2H), 3.64 (s, 1H), 3.60-3.52 (m, 1H), 3.43-3.32 (m, 1H), 2.22-2.08 (m, 5H), 1.80-1.59 (m, 4H), 1.44 (s, 9H), 1.26-1.06 (m, 4H).
    • Step 3
  • Figure US20200115354A1-20200416-C00264
    • tert-butyl 3-(((1S,2S)-2-(2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenoxy)cyclohexyl)(methyl)amino)azetidine-1-carboxylate
  • To a solution of tert-butyl 3-(((1S,2S)-2-hydroxycyclohexyl)(methyl)amino)azetidine-1-carboxylate (1.11 g, 3.91 mmol) in THF (30 mL) at 0° C. was added sodium hydride (60%, 195 mg, 4.88 mmol). The mixture was stirred at 0° C. for 0.5 h and 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide (1.5 g, 3.25 mmol) was added. The mixture was stirred at room temperature for an additional 16 h. EtOAc (100 mL) was added and washed with water (50 mL×2), brine (50 mL). The organic layer was dried over anhydrous MgSO4, filtered and concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 20-65%/0.225% formic acid in water) to give the title compound (200 mg, 9%) as a white solid. 1H NMR (400 MHz, CDCl3) δ 7.86 (d, J=7.2 Hz, 1H), 7.42 (d, J=3.6 Hz, 1H), 7.22 (d, J=8.0 Hz, 1H), 7.01 (d, J=3.6 Hz, 1H), 6.66 (d, J=11.6 Hz, 1H), 6.42-6.32 (m, 2H), 5.21 (s, 2H), 4.27-4.14 (m, 1H), 3.96-3.89 (m, 1H), 3.87-3.81 (m, 1H), 3.78-3.71 (m, 9H), 2.74-2.63 (m, 1H), 2.19-2.08 (m, 1H), 2.12 (s, 3H), 1.85-1.75 (m, 3H), 1.44 (s, 9H), 1.40-1.19 (m, 4H).
    • Step 4
  • Figure US20200115354A1-20200416-C00265
    • 4-(((1S,2S)-2-(azetidin-3-yl(methyl)amino)cyclohexyl)oxy)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • A mixture of tert-butyl 3-(((1S,2S)-2-(2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenoxy)cyclohexyl)(methyl)amino)azetidine-1-carboxylate (100 mg, 0.14 mmol) and formic acid (2 mL) was stirred at room temperature for 1 h. The mixture was concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 33-63%/0.225% formic acid in water) to give the title compound (32 mg, 46%) as a white solid. LCMS (ESI) m/z: 475.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.20 (s, 1H), 7.71 (d, J=7.6 Hz, 1H), 7.30 (d, J=11.6 Hz, 1H), 7.02 (d, J=4.0 Hz, 1H), 6.58 (d, J=4.0 Hz, 1H), 4.60-4.50 (m, 1H), 4.03-3.91 (m, 1H), 3.85-3.72 (m, 4H), 2.70-2.58 (m, 1H), 2.20 (s, 3H), 2.10-2.00 (m, 1H), 1.71-1.56 (m, 3H), 1.42-1.14 (m, 4H).
    • Example 67
  • Figure US20200115354A1-20200416-C00266
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-((S)-3-hydroxypyrrolidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 30 and making non-critical variations as required to replace 1,4-dibromobutane with 1,4-dibromobutan-2-ol, the title compound was obtained as a mixture of diastereomers. Separation of the mixture by chiral SFC afforded the title compound as a single diastereomer. The stereochemistry of the hydroxyl stereocenter was assigned arbitratrily. LCMS (ESI) m/z: 475.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 11.96 (s, 1H), 7.58 (d, J=7.3 Hz, 1H), 7.18 (d, J=4.4 Hz, 1H), 6.77-6.69 (m, 2H), 5.85 (dd, J=6.2, 1.8 Hz, 1H), 4.73 (s, 1H), 4.16 (dq, J=6.7, 3.3 Hz, 1H), 3.00 (dd, J=9.9, 5.8 Hz, 1H), 2.87 (s, 1H), 2.81 (s, 1H), 2.62 (d, J=10.0 Hz, 1H), 2.05 (d, J=16.7 Hz, 1H), 1.85 (ddd, J=20.2, 10.0, 4.8 Hz, 2H), 1.78 (q, J=11.0, 8.1 Hz, 2H), 1.64-1.50 (m, 2H), 1.46-1.24 (m, 2H), 1.28-1.12 (m, 2H).
    • Example 68
  • Figure US20200115354A1-20200416-C00267
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-((R)-3-hydroxypyrrolidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 30 and making non-critical variations as required to replace 1,4-dibromobutane with 1,4-dibromobutan-2-ol, the title compound was obtained as a mixture of diastereomers. Separation of the mixture by chiral SFC afforded the title compound as a single diastereomer. The stereochemistry of the hydroxyl stereocenter was assigned arbitrarily. LCMS (ESI) m/z: 475.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.59 (d, J=7.3 Hz, 1H), 7.19 (d, J=4.4 Hz, 1H), 6.77-6.69 (m, 2H), 5.89-5.82 (m, 1H), 4.74 (s, 1H), 4.11 (d, J=6.6 Hz, 1H), 2.79 (ddd, J=19.4, 15.7, 7.6 Hz, 4H), 2.55 (s, 1H), 2.07 (s, 2H), 1.87 (dd, J=12.9, 6.8 Hz, 2H), 1.77 (d, J=12.2 Hz, 1H), 1.60 (d, J=13.1 Hz, 1H), 1.61-1.48 (m, 1H), 1.34 (s, 1H), 1.43-1.21 (m, 3H), 1.25-1.12 (m, 1H).
    • Example 69
  • Figure US20200115354A1-20200416-C00268
    • 5-cyclopropyl-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 15 and making non-critical variations as required to replace 5-bromo-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide, 5-cyclopropyl-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 434.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.49 (s, 1H), 8.20 (d, J=6.4 Hz, 1H), 8.15 (s, 1H), 7.36 (d, J=8.0 Hz, 1H), 6.84 (d, J=5.6 Hz, 1H), 6.52 (d, J=13.6 Hz, 1H), 5.90 (s, 1H), 3.44-3.30 (m, 1H), 2.95-2.82 (m, 1H), 2.35 (s, 3H), 2.35 (s, 3H), 2.15-2.05 (m, 1H), 1.96-1.86 (m, 1H), 1.84-1.74 (m, 1H), 1.68-1.51 (m, 2H), 1.45-1.09 (m, 4H), 0.95-0.80 (m, 2H), 0.54-0.33 (m, 2H).
    • Example 70
  • Figure US20200115354A1-20200416-C00269
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(piperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide formate
  • Figure US20200115354A1-20200416-C00270
    Figure US20200115354A1-20200416-C00271
    • Step 1
  • Figure US20200115354A1-20200416-C00272
    • tert-butyl((1S,2R)-2-hydroxycyclohexyl)carbamate
  • To a solution of (1R,2S)-2-aminocyclohexanol hydrochloride (4.0 g, 26.38 mmol) and sodium hydroxide (2.11 g, 52.76 mmol) in dioxane (100 mL) and water (10 mL) at 0° C. was added di-tert-butyl dicarbonate (6.91 g, 31.66 mmol). The mixture was stirred at room temperature for 16 h and concentrated in vacuo. EtOAc (100 mL) was added and washed with water (50 mL×2), brine (30 mL). The organic layer was dried over anhydrous MgSO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (petroleum ether/EtOAc=4:1) to give the title compound (3.3 g, 58%) as colorless oil. 1H NMR (400 MHz, CDCl3) δ 4.60-4.51 (m, 1H), 3.36-3.24 (m, 3H), 2.10-1.93 (m, 2H), 1.73-1.66 (m, 2H), 1.46 (s, 9H), 1.37-1.08 (m, 4H).
    • Step 2
  • Figure US20200115354A1-20200416-C00273
    • (1R,2S)-2-((tert-butoxycarbonyl)amino)cyclohexyl methanesulfonate
  • To a solution of tert-butyl((1S,2R)-2-hydroxycyclohexyl)carbamate (3.3 g, 15.33 mmol) in DCM (100 mL) at 0° C. was added triethylamine (3.19 mL, 22.99 mmol) and methanesulfonyl chloride (3.8 g, 33.17 mmol). The mixture was stirred at room temperature for 16 h. The reaction mixture was washed with water (50 mL), 1N KHSO4 (50 mL), sat. aq. NaHCO3 (40 mL) and brine (40 mL). The organic layer was dried over anhydrous Na2SO4, filtered and concentrated in vacuo to give the title compound (3.5 g, 78%) as a white solid that required no further purification. 1H NMR (400 MHz, CDCl3) δ 4.98 (s, 1H), 4.81-4.72 (m, 1H), 3.75-3.60 (min, 1H), 3.03 (s, 3H), 2.21-2.12 (m, 1H), 1.83-1.69 (m, 2H), 1.63-1.49 (m, 4H), 1.48-1.30 (m, 10H).
    • Step 3
  • Figure US20200115354A1-20200416-C00274
    • tert-butyl((1S,2S)-2-(piperidin-1-yl)cyclohexyl)carbamate
  • A mixture of (1R,2S)-2-((tert-butoxycarbonyl)amino)cyclohexyl methanesulfonate (1.0 g, 3.41 mmol) and piperidine (7 mL) was heated to 60° C. for 24 h. After cooling to room temperature, the mixture was concentrated in vacuo. EtOAc (100 mL) was added and washed with water (50 mL×2), brine (50 mL). The organic layer was dried over anhydrous MgSO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (DCM/MeOH=20:1) to give the title compound (0.5 g, 52%) as a white solid. 1H NMR (400 MHz, CDCl3) δ 5.67-5.22 (m, 1H), 3.35-3.15 (m, 1H), 2.77-2.51 (m, 2H), 2.49-2.09 (m, 4H), 1.95-1.71 (m, 3H), 1.70-1.62 (m, 2H), 1.58-1.50 (m, 3H), 1.46 (s, 9H), 1.43-1.38 (m, 1H), 1.34-1.01 (m, 4H).
    • Step 4
  • Figure US20200115354A1-20200416-C00275
    • (1S,2S)-2-(piperidin-1-yl)cyclohexanamine 2,2,2-trifluoroacetate
  • To a solution of tert-butyl((1S,2S)-2-(piperidin-1-yl)cyclohexyl)carbamate (0.7 g, 2.48 mmol) in DCM (20 mL) was added trifluoroacetic acid (0.3 mL, 24.79 mmol). The mixture was stirred at room temperature for 16 h and concentrated in vacuo to give the title compound (0.8 g, crude) as light yellow oil that required no further purification.
    • Step 5
  • Figure US20200115354A1-20200416-C00276
    • 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(piperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of (1S,2S)-2-(piperidin-1-yl)cyclohexanamine 2,2,2-trifluoroacetate (489 mg, 1.65 mmol) and N,N-diisopropylethylamine (1.36 mL, 8.23 mmol) in DMF (15 mL) was added 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide (1.52 g, 3.29 mmol). The reaction mixture was stirred at room temperature for 16 h. The mixture was diluted with EtOAc (100 mL), washed with water (50 mL×2), brine (50 mL). The organic layer was dried over anhydrous MgSO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (petroleum ether/EtOAc=3:2) to give the title compound (0.25 g, 24%) as a white solid. LCMS (ESI) m/z: 623.1 [M+H]+.
    • Step 6:
  • Figure US20200115354A1-20200416-C00277
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(piperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide Formate
  • A mixture of 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(piperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (150 mg, 0.24 mmol) and formic acid (3.0 mL) was stirred at room temperature for 1 h. The reaction mixture was concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 8-38%/0.225% formic acid in water) to give the title compound (101 mg, 87%) as a white solid. LCMS (ESI) m/z: 473.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.14 (s, 1H), 7.61 (d, J=7.2 Hz, 1H), 7.26 (d, J=4.4 Hz, 1H), 6.82 (d, J=4.4 Hz, 1H), 6.76 (d, J=12.8 Hz, 1H), 6.04 (s, 1H), 3.37-3.33 (m, 1H), 2.75-2.65 (m, 1H), 2.64-2.55 (m, 2H), 2.48-2.42 (m, 2H), 2.20-2.05 (m, 1H), 1.95-1.85 (m, 1H), 1.80-1.73 (m, 1H), 1.67-1.57 (m, 1H), 1.53-1.45 (m, 2H), 1.40-1.34 (m, 4H), 1.31-1.11 (m, 4H).
    • Example 71
  • Figure US20200115354A1-20200416-C00278
    • 5-chloro-4-(((1S,2S)-2-(ethyl(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide
  • Following the procedure described in Example 18 and making non-critical variations as required to replace 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide, 5-chloro-4-(((1S,2S)-2-(ethyl(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 459.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.80 (q, J=8.2 Hz, 1H), 7.74 (d, J=7.4 Hz, 1H), 6.93-6.74 (m, 1H), 6.72-6.57 (m, 1H), 6.10 (s, 1H), 2.30-2.11 (m, 2H), 2.11-1.98 (m, 1H), 1.95-1.81 (m, 1H), 1.81-1.70 (m, 1H), 1.68-1.52 (m, 1H), 1.46-1.11 (m, 4H), 1.09-0.86 (m, 3H).
    • Example 72
  • Figure US20200115354A1-20200416-C00279
    • 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-((2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)benzenesulfonamide
  • Following the procedure described in Example 38 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(6-fluoropyridine-2-yl)benzenesulfonamide, the title compound was obtained as a white solid. LCMS (ESI) m/z: 475.1 [M+H]+. 1H NMR (400 MHz, DMSO-d) δ 11.30 (s, 1H), 7.79 (q, J=8.2 Hz, 1H), 7.71 (d, J=7.4 Hz, 1H), 6.91-6.79 (m, 1H), 6.73 (d, J=13.3 Hz, 1H), 6.64 (d, J=7.9 Hz, 1H), 6.10 (d, J=5.3 Hz, 1H), 4.32 (s, 1H), 3.51-3.35 (m, 3H), 2.60-2.52 (m, 2H), 2.17 (s, 3H), 2.13-2.03 (m, 1H), 1.90-1.68 (m, 2H), 1.65-1.54 (m, 1H), 1.45-1.04 (m, 4H).
    • Example 73
  • Figure US20200115354A1-20200416-C00280
    • 5-chloro-4-(((1S,2S)-2-(ethylamino)cyclohexyl)amino)-2-fluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide
  • Following the procedure described in Example 29, step 2 and making non-critical variations as required to replace the iodomethane with 1-iodoethane and 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(6-fluoropyridine-2-yl)benzenesulfonamide, the title compound was obtained. LCMS (ESI) m/z: 445.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.63 (d, J=7.4 Hz, 1H), 7.44 (d, J=8.3 Hz, 1H), 6.70 (d, J=12.9 Hz, 1H), 6.55 (d, J=7.9 Hz, 1H), 6.16 (s, 1H), 5.78 (s, 1H), 3.31 (d, J=8.5 Hz, 1H), 3.14 (s, 1H), 2.94 (s, 1H), 2.85 (s, 1H), 2.15-2.05 (m, 1H), 1.95-1.86 (m, 1H), 1.74 (d, J=12.5 Hz, 1H), 1.62 (d, J=12.8 Hz, 1H), 1.41-1.16 (m, 2H), 1.12 (t, J=7.2 Hz, 3H).
    • Example 74
  • Figure US20200115354A1-20200416-C00281
    • 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)benzenesulfonamide
  • Following the procedure described in Example 29, step 2 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(6-fluoropyridine-2-yl)benzenesulfonamide, the title compound was obtained. LCMS (ESI) m/z: 431.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.52-7.38 (m, 2H), 6.59 (dt, J=8.3, 4.2 Hz, 2H), 6.20-6.12 (m, 1H), 5.75 (d, J=9.6 Hz, 1H), 3.68 (s, 1H), 3.41 (d, J=11.0 Hz, 4H), 2.55 (s, 3H), 2.17-2.05 (m, 1H), 1.90 (d, J=12.1 Hz, 1H), 1.77 (d, J=13.0 Hz, 1H), 1.62 (d, J=13.1 Hz, 1H), 1.46 (s, 1H), 1.29-1.12 (m, 2H).
    • Example 75 & Example 83
  • Figure US20200115354A1-20200416-C00282
    • 5-chloro-4-(((1S,2S,5S)-2-(dimethylamino)-5-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1R,2R,5R)-2-(dimethylamino)-5-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Step 1
  • Figure US20200115354A1-20200416-C00283
    • (trans, trans)-5-chloro-4-((2-(dimethylamino)-5-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & (trans, cis)-5-chloro-4-((2-(dimethylamino)-5-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 48 and making non-critical variations as required to replace tert-butyl((1R,6R)-6-amino-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate with (trans)-tert-butyl(2-amino-4-(trifluoromethyl)cyclohexyl)carbamate, (trans, trans)-5-chloro-4-((2-(dimethylamino)-5-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (first peak on HPLC) and (trans, cis)-5-chloro-4-((2-(dimethylamino)-5-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (second peak on HPLC) were obtained both as white solid. First peak: LCMS (ESI) m/z: 501.1 [M+H]+. H NMR (400 MHz, DMSO-d6) δ 7.62 (d, J=7.2 Hz, 1H), 7.25 (d, J=4.4 Hz, 1H), 6.81 (d, J=4.4 Hz, 1H), 6.57 (d, J=12.8 Hz, 1H), 5.72 (d, J=6.4 Hz, 1H), 3.79-3.69 (m, 1H), 2.75-2.65 (m, 1H), 2.58-2.52 (m, 1H), 2.26 (s, 3H), 2.26 (s, 3H), 2.10-1.99 (m, 1H), 1.83-1.56 (m, 5H). Second peak: LCMS (ESI) m/z: 501.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.14 (s, 1H), 7.59 (d, J=7.2 Hz, 1H), 7.23 (d, J=4.4 Hz, 1H), 6.89 (d, J=12.8 Hz, 1H), 6.79 (d, J=4.4 Hz, 1H), 5.88 (d, J=6.0 Hz, 1H), 3.65-3.53 (m, 1H), 2.86-2.77 (m, 1H), 2.63-2.55 (m, 1H), 2.22 (s, 3H), 2.22 (s, 3H), 2.16-2.08 (m, 1H), 2.00-1.88 (m, 2H), 1.41-1.22 (m, 3H).
    • Step 2:
  • Figure US20200115354A1-20200416-C00284
    • 5-chloro-4-(((1S,2S,5S)-2-(dimethylamino)-5-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1R,2R,5R)-2-(dimethylamino)-5-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • (trans, cis)-5-chloro-4-((2-(dimethylamino)-5-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (93 mg) was separated by using chiral SFC (Chiralpak C2 (250 mm*30 mm, 10 um), Supercritical CO2/EtOH+0.1% NH4OH=55/45; 40 mL/min) to give 5-chloro-4-(((1S,2S,5S)-2-(dimethylamino)-5-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (53 mg, first peak) as a white solid and 5-chloro-4-(((1R,2R,5R)-2-(dimethylamino)-5-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (4 mg, second peak) as a white solid. Absolute configuration was arbitrarily assigned to each enantiomer. Example 75: LCMS (ESI) m/z: 501.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.59 (d, J=7.2 Hz, 1H), 7.22 (d, J=4.4 Hz, 1H), 6.88 (d, J=13.2 Hz, 1H), 6.77 (d, J=4.4 Hz, 1H), 5.85 (d, J=6.0 Hz, 1H), 3.62-3.51 (m, 1H), 2.82-2.73 (m, 1H), 2.65-2.53 (m, 1H), 2.20 (s, 3H), 2.20 (s, 3H), 2.17-2.09 (m, 1H), 1.99-1.88 (m, 2H), 1.40-1.22 (m, 3H). Example 83: LCMS (ESI) m/z: 501.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.15 (s, 1H), 7.59 (d, J=7.2 Hz, 1H), 7.22 (d, J=4.4 Hz, 1H), 6.88 (d, J=13.2 Hz, 1H), 6.77 (d, J=4.4 Hz, 1H), 5.85 (d, J=6.0 Hz, 1H), 3.62-3.51 (m, 1H), 2.82-2.73 (m, 1H), 2.65-2.53 (m, 1H), 2.20 (s, 3H), 2.20 (s, 3H), 2.17-2.09 (m, 1H), 1.99-1.88 (m, 2H), 1.40-1.22 (m, 3H).
    • Example 76 & Example 84
  • Figure US20200115354A1-20200416-C00285
    • 5-chloro-4-(((1S,2S,5R)-2-(dimethylamino)-5-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1R,2R,5S)-2-(dimethylamino)-5-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • (trans, trans)-5-chloro-4-((2-(dimethylamino)-5-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (51 mg) was separated by using chiral SFC (Chiralpak AS (250 mm*30 mm, 5 um), Supercritical CO2/EtOH+0.1% NH4OH=75/25; 50 mL/min) to give 5-chloro-4-(((1R,2R,5S)-2-(dimethylamino)-5-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (2 mg, first peak) as a white solid and 5-chloro-4-(((1S,2S,5R)-2-(dimethylamino)-5-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (28 mg, second peak) as a white solid. Absolute configuration was arbitrarily assigned to each enantiomer. Example 84: LCMS (ESI) m/z: 501.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.14 (s, 1H), 7.62 (d, J=7.2 Hz, 1H), 7.23 (d, J=4.4 Hz, 1H), 6.79 (d, J=4.4 Hz, 1H), 6.55 (d, J=12.8 Hz, 1H), 5.68 (d, J=6.4 Hz, 1H), 3.78-3.68 (m, 1H), 2.76-2.66 (m, 1H), 2.47-2.41 (m, 1H), 2.23 (s, 3H), 2.23 (s, 3H), 2.10-2.00 (m, 1H), 1.82-1.57 (m, 5H). Example 76: LCMS (ESI) m/z: 501.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.14 (s, 1H), 7.62 (d, J=7.2 Hz, 1H), 7.23 (d, J=4.4 Hz, 1H), 6.79 (d, J=4.4 Hz, 1H), 6.55 (d, J=12.8 Hz, 1H), 5.68 (d, J=6.4 Hz, 1H), 3.78-3.68 (m, 1H), 2.76-2.66 (m, 1H), 2.47-2.41 (m, 1H), 2.23 (s, 3H), 2.23 (s, 3H), 2.10-2.00 (m, 1H), 1.82-1.57 (m, 5H).
    • Example 78
  • Figure US20200115354A1-20200416-C00286
    • 4-(((1S,2S)-2-(dimethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)-5-(trifluoromethyl)benzenesulfonamide Formate
  • Following the procedure described in Example 66 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide and tert-butyl 3-(((1S,2S)-2-hydroxycyclohexyl)(methyl)amino)azetidine-1-carboxylate with N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)-5-(trifluoromethyl)benzenesulfonamide and (1S,2S)-2-(dimethylamino)cyclohexanol, 4-(((1S,2S)-2-(dimethylamino)cyclohexyl)oxy)-2-fluoro-N-(thiazol-2-yl)-5-(trifluoromethyl)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 468.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.14 (s, 1H), 7.94 (d, J=7.6 Hz, 1H), 7.54 (d, J=12.0 Hz, 1H), 7.21 (d, J=4.4 Hz, 1H), 6.77 (d, J=4.4 Hz, 1H), 4.84-4.74 (m, 1H), 2.67-2.57 (m, 1H), 2.26 (s, 3H), 2.26 (s, 3H), 2.05-1.95 (m, 1H), 1.85-1.75 (m, 1H), 1.71-1.55 (m, 2H), 1.41-1.17 (m, 4H).
    • Example 79
  • Figure US20200115354A1-20200416-C00287
    • 2-(((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)(methyl)amino)acetamide Formate
  • Following the procedure described in Example 29 and making non-critical variations as required to replace iodopropane with 2-chloroacetamide, 2-(((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)(methyl)amino)acetamide formate was obtained as a white solid. LCMS (ESI) m/z: 476.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.15 (s, 1H), 7.56 (d, J=7.6 Hz, 1H), 7.24 (d, J=4.4 Hz, 1H), 7.11 (d, J=3.2 Hz, 1H), 6.95 (s, 1H), 6.79 (d, J=4.8 Hz, 1H), 6.71 (d, J=13.2 Hz, 1H), 6.17 (d, J=6.4 Hz, 1H), 3.45-3.31 (m, 1H), 3.06-2.97 (m, 1H), 2.95-2.87 (m, 1H), 2.72-2.63 (m, 1H), 2.14 (s, 3H), 2.05-1.97 (m, 1H), 1.85-1.75 (m, 1H), 1.73-1.67 (m, 1H), 1.63-1.53 (m, 1H), 1.38-1.09 (m, 4H).
    • Example 80
  • Figure US20200115354A1-20200416-C00288
    • 2-(((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)(methyl)amino)-N-methylacetamide Formate
  • Following the procedure described in Example 29 and making non-critical variations as required to replace iodopropane with 2-chloro-N-methylacetamide, 2-(((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)(methyl)amino)-N-methylacetamide formate was obtained as a white solid. LCMS (ESI) m/z: 490.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.15 (s, 1H), 7.58 (d, J=7.6 Hz, 1H), 7.28 (d, J=4.4 Hz, 1H), 7.23 (d, J=4.8 Hz, 1H), 6.79 (d, J=4.8 Hz, 1H), 6.72 (d, J=13.2 Hz, 1H), 6.07 (d, J=6.8 Hz, 1H), 3.44-3.37 (m, 1H), 3.06 (d, J=16.0 Hz, 1H), 2.86 (d, J=16.0 Hz, 1H), 2.73-2.62 (m, 1H), 2.44 (d, J=5.2 Hz, 3H), 2.14 (s, 3H), 2.01-1.94 (m, 1H), 1.87-1.79 (m, 1H), 1.78-1.68 (m, 1H), 1.63-1.55 (m, 1H), 1.40-1.13 (m, 4H).
    • Example 85
  • Figure US20200115354A1-20200416-C00289
    • 5-chloro-N-(6-chloropyridin-2-yl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluorobenzenesulfonamide
  • Following the procedure described in Example 11 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide with 5-chloro-N-(6-chloropyridin-2-yl)-N-(2,4-dimethoxybenzyl)-2,4-difluorobenzenesulfonamide, 5-chloro-N-(6-chloropyridin-2-yl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluorobenzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 461.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.85-7.73 (m, 1H), 7.68-7.53 (m, 1H), 7.11-6.73 (m, 3H), 6.26-5.92 (m, 1H), 3.43-3.39 (m, 1H), 3.32-3.26 (m, 1H), 2.47-2.29 (m, 6H), 2.00-1.85 (m, 2H), 1.87-1.69 (m, 1H), 1.67-1.51 (m, 1H), 1.28 (ddd, J=51.9, 15.9, 9.4 Hz, 4H).
    • Example 86
  • Figure US20200115354A1-20200416-C00290
    • 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-((2-hydroxypropyl)(methyl)amino)cyclohexyl)amino)benzenesulfonamide
  • Following the procedure described in Example 29 and making non-critical variations as required to replace the iodopropane with 1-bromo-2-propanol and 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide, the title compound was obtained. LCMS (ESI) m/z: 489.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 11.47 (s, 1H), 7.81 (s, 1H), 7.71 (d, J=7.4 Hz, 1H), 6.84 (s, 1H), 6.75 (d, J=13.2 Hz, 1H), 6.67 (s, 1H), 6.06 (s, 1H), 4.24 (s, 1H), 3.62 (s, 1H), 3.49-3.24 (m, 2H), 3.35 (s, 1H), 2.54 (s, 2H), 2.40 (s, 1H), 2.27 (s, 1H), 2.15 (s, 2H), 2.08 (s, 3H), 1.81 (s, 1H), 1.74 (d, J=10.1 Hz, 1H), 1.58 (d, J=12.9 Hz, 1H), 1.33 (t, J=12.7 Hz, 1H), 1.22 (t, J=10.7 Hz, 1H), 1.14 (s, 2H), 1.00 (d, J=6.1 Hz, 1H), 0.84 (d, J=5.9 Hz, 2H).
    • Example 88 & Example 103
  • Figure US20200115354A1-20200416-C00291
    • 5-chloro-4-(((1S,2S,5R)-2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1R,2R,5S)-2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Step 1
  • Figure US20200115354A1-20200416-C00292
    • (trans)-tert-butyl(2-amino-4-(3-(trifluoromethyl)phenyl)cyclohexyl)carbamate
  • Following the procedure described in Example 36, step 1 and making non-critical variations as required to replace (trans)-tert-butyl(6-amino-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate with (trans)-tert-butyl(3-amino-3′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate, (trans)-tert-butyl(2-amino-4-(3-(trifluoromethyl)phenyl)cyclohexyl)carbamate was obtained as a light yellow solid. LCMS (ESI) m/z: 359.1 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00293
    • (trans, trans)-5-chloro-4-((2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & (trans, cis)-5-chloro-4-((2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 48 and making non-critical variations as required to replace tert-butyl((1R,6R)-6-amino-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate with (trans)-tert-butyl (2-amino-4-(3-(trifluoromethyl)phenyl)cyclohexyl)carbamate, (trans, trans)-5-chloro-4-((2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (first peak on HPLC) and (trans, cis)-5-chloro-4-((2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (second peak on HPLC) were obtained both as white solid.
    • Step 3
  • Figure US20200115354A1-20200416-C00294
    • 5-chloro-4-(((1S,2S,5R)-2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1R,2R,5S)-2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • (trans, trans)-5-chloro-4-((2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (65 mg) was separated by using chiral SFC (Chiralpak AS (250 mm*30 mm, 5 um), Supercritical CO2/EtOH+0.1% NH4OH=60/40; 50 mL/min) to give 5-chloro-4-(((1S,2S,5R)-2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (7 mg, first peak) as a white solid and 5-chloro-4-(((1R,2R,5S)-2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (35 mg, second peak) as a white solid. Absolute configuration was arbitrarily assigned to each enantiomer.
  • Example 88: LCMS (ESI) m/z: 577.3 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.70-7.66 (m, 2H), 7.62 (d, J=7.2 Hz, 1H), 7.60-7.57 (m, 2H), 7.21 (d, J=4.4 Hz, 1H), 6.77 (d, J=4.8 Hz, 1H), 6.41 (d, J=12.4 Hz, 1H), 5.68 (d, J=6.0 Hz, 1H), 3.63-3.57 (m, 1H), 3.17-3.12 (m, 1H), 2.47-2.41 (m, 1H), 2.35-2.30 (m, 1H), 2.25 (s, 3H), 2.25 (s, 3H), 2.10-1.97 (m, 1H), 1.82-1.65 (m, 4H). Example 103: LCMS (ESI) m/z: 577.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.70-7.66 (m, 2H), 7.62 (d, J=7.2 Hz, 1H), 7.60-7.57 (m, 2H), 7.21 (d, J=4.4 Hz, 1H), 6.77 (d, J=4.8 Hz, 1H), 6.41 (d, J=12.4 Hz, 1H), 5.68 (d, J=6.0 Hz, 1H), 3.63-3.57 (m, 1H), 3.17-3.12 (m, 1H), 2.47-2.41 (m, 1H), 2.35-2.30 (m, 1H), 2.25 (s, 3H), 2.25 (s, 3H), 2.10-1.97 (m, 1H), 1.82-1.65 (m, 4H).
    • Example 87 & Example 93
  • Figure US20200115354A1-20200416-C00295
    • 5-chloro-4-(((1S,2S,5S)-2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1R,2R,5R)-2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • (trans, cis)-5-chloro-4-((2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (30 mg) was separated by using chiral SFC (Chiralpak OJ (250 mm*30 mm, 5 um), Supercritical CO2/EtOH+0.1% NH4OH=80/20; 60 mL/min) to give 5-chloro-4-(((1S,2S,5S)-2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (9 mg, first peak) as a white solid and 5-chloro-4-(((1R,2R,5R)-2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (2 mg, second peak) as a white solid. Absolute configuration was arbitrarily assigned to each enantiomer.
  • Example 87: LCMS (ESI) m/z: 577.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.60-7.51 (m, 5H), 7.19 (d, J=4.8 Hz, 1H), 6.88 (d, J=13.2 Hz, 1H), 6.74 (d, J=4.4 Hz, 1H), 5.87 (d, J=5.2 Hz, 1H), 3.65-3.58 (m, 1H), 3.00-2.85 (m, 2H), 2.29 (s, 3H), 2.29 (s, 3H), 2.23-2.15 (m, 1H), 2.01-1.90 (m, 2H), 1.58-1.43 (m, 3H). Example 93: LCMS (ESI) m/z: 577.3 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.60-7.51 (m, 5H), 7.17 (d, J=4.8 Hz, 1H), 6.86 (d, J=13.2 Hz, 1H), 6.71 (d, J=4.4 Hz, 1H), 5.84 (d, J=4.4 Hz, 1H), 3.65-3.58 (m, 1H), 3.00-2.85 (m, 2H), 2.27 (s, 3H), 2.27 (s, 3H), 2.23-2.15 (m, 1H), 2.01-1.90 (m, 2H), 1.58-1.43 (m, 3H).
    • Example 89
  • Figure US20200115354A1-20200416-C00296
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-((2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 38 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide, 5-chloro-2-fluoro-4-(((1S,2S)-2-((2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 458.3 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.46 (s, 1H), 8.18-8.12 (m, 2H), 7.66 (d, J=7.2 Hz, 1H), 6.79 (d, J=6.0 Hz, 1H), 6.71 (d, J=12.8 Hz, 1H), 5.93 (d, J=5.2 Hz, 1H), 3.50-3.40 (m, 4H), 2.86-2.83 (m, 1H), 2.69-2.56 (m, 2H), 2.28 (s, 3H), 2.07-2.08 (m, 1H), 1.86-1.89 (m, 1H), 1.76-1.78 (m, 1H), 1.58-1.61 (m, 1H), 1.38-1.10 (m, 4H).
    • Example 90
  • Figure US20200115354A1-20200416-C00297
    • 4-(((1S,2S)-2-(azetidin-3-yl(methyl)amino)cyclohexyl)oxy)-5-cyclopropyl-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 15 and making non-critical variations as required to replace 5-bromo-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with tert-butyl 3-(((1S,2S)-2-(2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenoxy)cyclohexyl)(methyl)amino)azetidine-1-carboxylate, 4-(((1S,2S)-2-(azetidin-3-yl(methyl)amino)cyclohexyl)oxy)-5-cyclopropyl-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 481.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.26 (s, 1H), 7.19 (d, J=8.0 Hz, 1H), 7.13-6.95 (m, 2H), 6.61 (s, 1H), 4.50-4.40 (m, 1H), 4.06-4.03 (m, 1H), 3.71-3.62 (m, 4H), 2.70-2.60 (m, 1H), 2.27 (s, 3H), 2.09-1.97 (m, 2H), 1.74-1.55 (m, 3H), 1.46-1.09 (m, 4H), 0.97-0.83 (m, 2H), 0.60-0.42 (m, 2H).
    • Example 91
  • Figure US20200115354A1-20200416-C00298
    • 5-cyclobutyl-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00299
    • Step 1
  • Figure US20200115354A1-20200416-C00300
    • 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide (1.04 g, 2.25 mmol) and K2CO3 (0.93 g, 6.75 mmol) in DMF (25 mL) was added (1S,2S)—N1,N1-dimethylcyclohexane-1,2-diamine (0.32 g, 2.25 mmol). The reaction mixture was stirred at room temperature for 16 h. The mixture was diluted with EtOAc (100 mL), washed with water (100 mL×3), brine (100 mL), dried over anhydrous Na2SO4, filtered and concentrated in vacuo to give the title compound (1.2 g, crude) as a brown solid that required no further purification. LCMS (ESI) m/z: 583.1 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00301
    • 5-cyclobutyl-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (500 mg, 0.86 mmol) in toluene (5 mL) and water (1 mL) was added chloro[(di(1-adamantyl)-n-butylphosphine)-2-(2-aminobiphenyl)]palladium(II) (115 mg, 0.17 mmol), potassium cyclobutyltrifluoroboranuide (280 mg, 1.71 mmol) and Cs2CO3 (839 mg, 2.57 mmol). The reaction mixture was heated to 100° C. for 16 h. After cooling to room temperature, the mixture was concentrated in vacuo. EtOAc (50 mL) was added, washed with water (50 mL×2), brine (50 mL), dried over anhydrous MgSO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (petroleum ether/EtOAc=2:1)) to give the title compound (30 mg, 6%) as a white solid. LCMS (ESI) m/z: 603.3 [M+H]+.
    • Step 3
  • Figure US20200115354A1-20200416-C00302
    • 5-cyclobutyl-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • A mixture of 5-cyclobutyl-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (25 mg, 0.04 mmol) and formic acid (1.0 mL) was stirred at room temperature for 1 h. The reaction mixture was concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 12-42%/0.225% formic acid in water) to give the title compound (7 mg, 36%) as a white solid. LCMS (ESI) m/z: 453.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.36 (d, J=8.0 Hz, 1H), 7.19 (d, J=4.4 Hz, 1H), 6.74 (d, J=4.4 Hz, 1H), 6.45 (d, J=13.2 Hz, 1H), 5.25 (s, 1H), 3.24-3.21 (m, 2H), 2.70-2.60 (m, 1H), 2.40-2.28 (m, 2H), 2.22 (s, 3H), 2.22 (s, 3H), 2.17-1.94 (m, 3H), 1.91-1.74 (m, 4H), 1.65-1.70 (m 1H), 1.40-1.30 (m, 1H), 1.25-1.19 (m, 2H), 1.14-0.97 (m, 1H).
    • Example 92
  • Figure US20200115354A1-20200416-C00303
    • 5-chloro-2-fluoro-N-(4-methylthiazol-2-yl)-4-(((1S,2S)-2-(pyrrolidin-1-yl)cyclohexyl)amino)benzenesulfonamide Formate
  • Following the procedure described in Example 30 and non-critical variations required to substitute 4-[[(1S,2S)-2-aminocyclohexyl]amino]-5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide with 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(4-methylthiazol-2-yl)benzenesulfonamide, the title compound was obtained as a white solid. LCMS (ESI) m/z: 473.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.16 (s, 1H), 7.57 (d, J=7.2 Hz, 1H), 6.76 (d, J=13.2 Hz, 1H), 6.33 (d, J=1.2 Hz, 1H), 5.92 (d, J=6.0 Hz, 1H), 3.45-3.34 (m, 2H), 3.01-2.90 (m, 1H), 2.76-2.64 (m, 4H), 2.07 (s, 3H), 2.06-2.02 (m, 1H), 1.92-1.86 (m, 1H), 1.79-1.73 (m, 1H), 1.66-1.62 (m, 4H), 1.39-1.32 (m, 2H), 1.29-1.18 (m, 2H).
    • Example 94
  • Figure US20200115354A1-20200416-C00304
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(methyl((S)-3,3,3-trifluoro-2-hydroxypropyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 29 and making non-critical variations as required to replace the iodopropane with 3-bromo-1,1,1-trifluoro-2-propanol the title compound was obtained as a mixture of diastereomers. Separation of isomers by HPLC (acetonitrile 26-56%/0.225% formic acid in water) provided the title compound. The stereochemistry of alcohol stereocenter was assigned arbitrarily. LCMS (ESI) m/z: 531.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.73 (s, 1H), 7.57 (d, J=7.3 Hz, 1H), 7.24 (d, J=4.6 Hz, 1H), 6.80 (d, J=4.6 Hz, 1H), 6.69 (d, J=13.1 Hz, 1H), 5.98-5.88 (m, 2H), 3.94 (s, 1H), 2.69 (td, J=14.9, 14.2, 5.3 Hz, 2H), 2.60-2.51 (m, 1H), 2.15 (s, 3H), 2.13-2.06 (m, 1H), 1.77 (t, J=14.4 Hz, 2H), 1.59 (d, J=13.2 Hz, 1H), 1.26 (s, 2H), 1.42-1.04 (m, 2H).
    • Example 95
  • Figure US20200115354A1-20200416-C00305
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(methyl((R)-3,3,3-trifluoro-2-hydroxypropyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 29 and making non-critical variations as required to replace the iodopropane with 3-bromo-1,1,1-trifluoro-2-propanol the title compound was obtained as a mixture of diastereomers. Separation of isomers by HPLC (acetonitrile 26-56%/0.225% formic acid in water) provided the title compound. The stereochemistry of alcohol stereocenter was assigned arbitrarily. LCMS (ESI) m/z: 531.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.73 (s, 1H), 7.57 (d, J=7.3 Hz, 1H), 7.24 (d, J=4.6 Hz, 1H), 6.80 (d, J=4.5 Hz, 1H), 6.70 (d, J=13.0 Hz, 1H), 5.94 (s, 1H), 5.89-5.82 (m, 1H), 4.00 (s, 1H), 2.76-2.57 (m, 3H), 2.16 (s, 3H), 2.09 (d, J=12.7 Hz, 1H), 1.77 (dd, J=22.0, 10.6 Hz, 2H), 1.59 (d, J=13.1 Hz, 1H), 1.41-1.22 (m, 2H), 1.18-1.04 (m, 1H).
    • Example 96
  • Figure US20200115354A1-20200416-C00306
    • 5-cyclopropyl-4-(((1S,2S)-2-(ethylamino)cyclohexyl)oxy)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00307
    Figure US20200115354A1-20200416-C00308
    • Step 1
  • Figure US20200115354A1-20200416-C00309
    • 5-cyclopropyl-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 15, step 1 and making non-critical variations as required to replace 5-bromo-N-[(2,4-dimethoxyphenyl)methyl]-4-[[(1S,2S)-2-(dimethylamino)cyclohexyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide, 5-cyclopropyl-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide was obtained as a pale yellow solid. 1H NMR (400 MHz, CDCl3) δ 8.81-8.73 (m, 1H), 8.45 (d, J=6.0 Hz, 1H), 7.65-7.58 (m, 1H), 7.22-7.14 (m, 2H), 6.86-6.79 (m, 1H), 6.46-6.39 (m, 2H), 5.24 (s, 2H), 3.80 (s, 3H), 3.78 (s, 3H), 2.11-2.00 (m, 1H), 1.11-1.04 (m, 2H), 0.81-0.74 (m, 2H).
    • Step 2
  • Figure US20200115354A1-20200416-C00310
    • (1S,2S)-2-(benzylamino)cyclohexanol
  • To a solution of (1S,2S)-2-aminocyclohexanol (5.0 g, 43.41 mmol) in DCM (20 mL) was added benzaldehyde (4.61 g, 43.41 mmol). The mixture was stirred at room temperature for 0.5 h and magnesium sulfate (7.84 g, 65.12 mmol) was added portionwise. The mixture was stirred at room temperature for an additional 2.5 h. The reaction was filtered and sodium borohydride (3.72 g, 98.39 mmol) at 0° C. was added portionwise. The mixture was stirred at 0° C. for 3 h. The reaction was quenched with sat. aq. NH4Cl (20 mL) slowly. The organic layer was dried over anhydrous Na2SO4, filtered and concentrated in vacuo to give the title compound (5 g, crude) as colorless oil that required no further purification. 1H NMR (400 MHz, CDCl3) δ 7.38-7.32 (m, 4H), 7.30-7.25 (m, 1H), 4.00-3.97 (m, 1H), 3.74-3.70 (m, 1H), 3.28-3.22 (m, 1H), 2.37-2.31 (m, 1H), 2.20-2.16 (m, 1H), 2.05-2.03 (m, 1H), 1.76-1.72 (m, 2H), 1.35-1.18 (m, 3H), 1.11-1.02 (m, 1H).
    • Step 3
  • Figure US20200115354A1-20200416-C00311
    • (1S,2S)-2-(benzyl(ethyl)amino)cyclohexanol
  • To a solution of (1S,2S)-2-(benzylamino)cyclohexanol (5.0 g, 24.35 mmol) in EtOH (50 mL) was added acetaldehyde (5.42 g, 121.77 mmol). The mixture was stirred at room temperature for 0.5 h and sodium cyanoborohydride (3.06 g, 48.71 mmol) was added. The mixture was stirred at room temperature for an additional 12 h. The reaction was quenched with sat. aq. NH4Cl (20 mL) and extracted with EtOAc (50 mL). The organic layer was dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (solvent gradient: 0-10% EtOAc in petroleum ether) to give the title compound (1.0 g, 17%) as yellow oil. 1H NMR (400 MHz, CDCl3) δ 7.35-7.25 (m, 5H), 4.05-3.91 (m, 1H), 3.86 (d, J=13.6 Hz, 1H), 3.47-3.37 (m, 1H), 3.36 (d, J=13.6 Hz, 1H), 2.70-2.59 (m, 1H), 2.49-2.35 (m, 2H), 2.13-2.12 (m, 1H), 1.90-1.85 (m, 1H), 1.81-1.79 (m, 1H), 1.72-1.70 (m, 1H), 1.29-1.16 (m, 4H), 1.06 (t, J=7.2 Hz, 3H).
    • Step 4
  • Figure US20200115354A1-20200416-C00312
    • (1S,2S)-2-(ethylamino)cyclohexanol
  • To a solution of (1S,2S)-2-(benzyl(ethyl)amino)cyclohexanol (1.0 g, 4.29 mmol) in EtOH (10 mL) and AcOH (0.1 mL) was added Pd/C (10%) (0.2 g, 0.19 mmol). The mixture was heated to 40° C. for 16 h under hydrogen atmosphere (50 psi). The mixture was filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (solvent gradient: 0-10% EtOAc in petroleum ether) to give the title compound (0.5 g, 82%) as yellow oil.
    • Step 5
  • Figure US20200115354A1-20200416-C00313
    • tert-butyl ethyl((1S,2S)-2-hydroxycyclohexyl)carbamate
  • To a solution of (1S,2S)-2-(ethylamino)cyclohexanol (0.5 g, 3.49 mmol) in DCM (15 mL) was added sodium bicarbonate (1.47 g, 17.45 mmol) in water (5 mL) and di-tert-butyl dicarbonate (914 mg, 4.19 mmol) slowly. The reaction mixture was stirred at room temperature for 12 h. The organic layer was concentrated in vacuo. The crude residue was purified by silica gel chromatography (solvent gradient: 0-10% EtOAc in petroleum ether) to give the title compound (0.3 g, 35%) as yellow oil.
    • Step 6
  • Figure US20200115354A1-20200416-C00314
    • tert-butyl((1S,2S)-2-(2-cyclopropyl-4-(N-(2,4-dimethoxybenzyl)-N-(pyrimidin-4-yl)sulfamoyl)-5-fluorophenoxy)cyclohexyl)(ethyl)carbamate
  • To a solution of tert-butyl ethyl((1S,2S)-2-hydroxycyclohexyl)carbamate (255 mg, 1.05 mmol) in THF (5 mL) at 0° C. was added sodium hydride (60%, 57 mg, 1.43 mmol). The mixture was stirred at 0° C. for 0.5 h and 5-cyclopropyl-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide (0.44 g, 0.95 mmol) was added. The mixture was stirred at room temperature for an additional 1 h. The mixture was quenched with sat. aq. NH4Cl (3 mL) slowly at 0° C. and extracted with EtOAc (10 mL×2). The combined organic layers were dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (petroleum ether/EtOAc=20:1) to give the title compound (0.2 g, 31%) as colorless oil. LCMS (ESI) m/z: 685.1 [M+H]+.
    • Step 7
  • Figure US20200115354A1-20200416-C00315
    • 5-cyclopropyl-4-(((1S,2S)-2-(ethylamino)cyclohexyl)oxy)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • A mixture of tert-butyl((1S,2S)-2-(2-cyclopropyl-4-(N-(2,4-dimethoxybenzyl)-N-(pyrimidin-4-yl)sulfamoyl)-5-fluorophenoxy)cyclohexyl)(ethyl)carbamate (200 mg, 0.29 mmol) and formic acid (3 mL) was stirred at room temperature for 1 h. The mixture was concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 10-20%/0.225% formic acid in water) to give the title compound (15 mg, 11%) as a white solid. LCMS (ESI) m/z: 435.3 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.25 (s, 1H), 7.91 (d, J=6.0 Hz, 1H), 7.22 (d, J=8.4 Hz, 1H), 6.98 (d, J=12.0 Hz, 1H), 6.54 (d, J=6.4 Hz, 1H), 4.51-4.37 (m, 1H), 3.24-3.23 (m, 3H), 3.03-3.01 (m, 1H), 2.13-2.01 (m, 3H), 1.73-1.61 (m, 2H), 1.40-1.21 (m, 4H), 1.16 (t, J=6.8 Hz, 3H), 0.90-0.80 (m, 2H), 0.55-0.45 (m, 2H).
    • Example 97
  • Figure US20200115354A1-20200416-C00316
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(methyl(oxetan-3-yl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 39 and making non-critical variations as required to replace tert-butyl 3-oxoazetidine-1-carboxylate with oxetan-3-one, 5-chloro-2-fluoro-4-(((1S,2S)-2-(methyl(oxetan-3-yl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 475.1 [M+H]+. H NMR (400 MHz, DMSO-d6) δ 12.77 (s, 1H), 7.58 (d, J=7.2 Hz, 1H), 7.27 (d, J=4.8 Hz, 1H), 6.83 (d, J=4.8 Hz, 1H), 6.76 (d, J=12.8 Hz, 1H), 5.83 (d, J=5.2 Hz, 1H), 4.50-4.38 (m, 3H), 4.35-4.32 (m, 1H), 4.05-3.94 (m, 1H), 3.36-3.35 (m, 1H), 2.54-2.53 (m, 1H), 2.06 (s, 3H), 2.04-1.97 (m, 1H), 1.75-1.54 (m, 3H), 1.36-1.26 (m, 1H), 1.26-1.12 (m, 3H).
    • Example 98 & Example 99
  • Figure US20200115354A1-20200416-C00317
    • 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1R,2R)-2-(pyrrolidin-1-yl)cyclohexyl)amino)benzenesulfonamide & 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-(pyrrolidin-1-yl)cyclohexyl)amino)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00318
    • Step 1
  • Figure US20200115354A1-20200416-C00319
    • Racemic trans-2-(pyrrolidin-1-yl)cyclohexan-1-amine
  • The mixture of 7-azabicyclo[4.1.0]heptane (80 mg, 0.823 mmol), pyrrolidine (117 mg, 1.65 mmol) and NH4Cl (4.4 mg, 0.0823 mmol) in water (0.5 mL) in a sealed microwave tube was heated at 105° C. for 17 h. The mixture was cooled, extracted with DCM (2×). The combined organics were dried (Na2SO4), filtered and concentrated to give crude racemic trans-2-pyrrolidin-1-ylcyclohexanamine (100 mg, 72.2%) as a brown oil.
  • This residue was dissolved in MeOH (2 mL), Boc2O (156 mg, 0.713 mmol) was then added. The mixture was stirred at room temperature for 4 h, and then concentrated. The residue was partitioned between EtOAc and 10% citric acid. The aq layer was extracted with 10% citric acid one more time. The combined aq layers were basified with sat. Na2CO3, extracted with EtOAc (3×). The combined organics were dried (Na2SO4), filtered and concentrated to a brown oil. LCMS (ESI) m/z: 269 [M+H+]. 1H NMR (400 MHz, Chloroform-d) δ 5.21 (d, J=6.2 Hz, 1H), 3.38-3.22 (m, 1H), 2.71-2.46 (m, 4H), 2.45-2.26 (m, 2H), 1.82-1.53 (m, 7H), 1.49-1.41 (m, 9H), 1.37-0.99 (m, 4H).
  • This oil was dissolved in THF (3 mL). HCl (4 mol/L) in 1,4-dioxane (2.6 mL) was added. The mixture was stirred at room temperature for 20 h, then concentrated and dried in high vacuum to give a brown oil which was used in the next step without purification.
    • Step 2
  • Figure US20200115354A1-20200416-C00320
    • 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(3-fluorophenyl)-4-(((1R,2R)-2-(pyrrolidin-1-yl)cyclohexyl)amino)benzenesulfonamide & 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(3-fluorophenyl)-4-(((1S,2S)-2-(pyrrolidin-1-yl)cyclohexyl)amino)benzenesulfonamide
  • The mixture of 5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2,4-difluoro-N-(6-fluoro-2-pyridyl)benzenesulfonamide (180 mg, 0.381 mmol), racemic trans-2-(pyrrolidin-1-yl)cyclohexan-1-amine, and K2CO3 (292 mg, 2.115 mmol) in DMF (2 mL) was stirred at room temperature for 17 h. The mixture was partitioned between iPrOAc and water. The aq layer was extracted with iPrOAc (2×). The combined organics were dried (Na2SO4), filtered and concentrated. The crude product was purified by chiral SFC to give 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(3-fluorophenyl)-4-(((1R,2R)-2-(pyrrolidin-1-yl)cyclohexyl)amino)benzenesulfonamide (first peak, 57 mg, 23.9%) & 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(3-fluorophenyl)-4-(((1S,2S)-2-(pyrrolidin-1-yl)cyclohexyl)amino)benzenesulfonamide (second peak, 81 mg, 34.3%) as white solids. Absolute configuration was arbitrarily assigned to each enantiomer. LCMS (ESI) m/z: 621.2 [M+H]+.
    • Step 3
  • Figure US20200115354A1-20200416-C00321
    • 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1R,2R)-2-(pyrrolidin-1-yl)cyclohexyl)amino)benzenesulfonamide
  • Following the procedure described in Example 1, Step 3 and making non-critical variations as required to replace tert-butyl((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)carbamate with 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(3-fluorophenyl)-4-(((1R,2R)-2-(pyrrolidin-1-yl)cyclohexyl)amino)benzenesulfonamide, 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1R,2R)-2-(pyrrolidin-1-yl)cyclohexyl)amino)benzenesulfonamide was obtained as a white solid. Example 98: LCMS (ESI) m/z: 471.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 11.52 (s, 1H), 9.03 (s, 1H), 7.86 (q, J=8.2 Hz, 1H), 7.79 (d, J=7.4 Hz, 1H), 7.03 (d, J=13.6 Hz, 1H), 6.89 (dd, J=7.9, 2.0 Hz, 1H), 6.73 (dd, J=8.0, 2.4 Hz, 1H), 6.50-6.33 (m, 1H), 3.97-3.77 (m, 1H), 3.62 (t, J=11.1 Hz, 1H), 3.12-2.94 (m, 1H), 2.10 (s, 6H), 1.91-1.73 (m, 6H), 1.68-1.55 (m, 1H), 1.49-1.12 (m, 5H).
  • Figure US20200115354A1-20200416-C00322
    • 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-(pyrrolidin-1-yl)cyclohexyl)amino)benzenesulfonamide
  • Following the procedure described in Example 1, Step 3 and making non-critical variations as required to replace tert-butyl((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)carbamate with 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(3-fluorophenyl)-4-(((1S,2S)-2-(pyrrolidin-1-yl)cyclohexyl)amino)benzenesulfonamide, 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-(pyrrolidin-1-yl)cyclohexyl)amino)benzenesulfonamide was obtained as a white solid. Example 99: LCMS (ESI) m/z: 471.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 11.52 (s, 1H), 7.86 (q, J=8.2 Hz, 1H), 7.78 (d, J=7.5 Hz, 1H), 7.03 (d, J=13.6 Hz, 1H), 6.89 (dd, J=8.0, 2.1 Hz, 1H), 6.73 (dd, J=8.0, 2.4 Hz, 1H), 6.41 (d, J=10.0 Hz, 1H), 3.95-3.79 (m, 1H), 3.70-3.56 (m, 1H), 3.16-2.94 (m, 1H), 1.94-1.77 (m, 7H), 1.68-1.56 (m, 1H), 1.51-1.15 (m, 4H).
    • Example 100
  • Figure US20200115354A1-20200416-C00323
    • 5-cyclopropyl-4-(((1S,2S)-2-(ethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 29 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide and iodomethane with 5-cyclopropyl-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide and iodoethane, the title compound was obtained as a white solid. LCMS (ESI) m/z: 434.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.31 (s, 1H), 7.94 (d, J=6.0 Hz, 1H), 7.29 (d, J=8.4 Hz, 1H), 6.62 (d, J=5.6 Hz, 1H), 6.48 (d, J=13.6 Hz, 1H), 5.43 (d, J=9.2 Hz, 1H), 3.23-3.20 (m, 1H), 3.06-2.85 (m, 2H), 2.64-2.57 (m, 1H), 2.19-2.09 (m, 1H), 2.00-1.90 (m, 1H), 1.80-1.70 (m, 1H), 1.67-1.55 (m, 2H), 1.45-1.32 (m, 2H), 1.30-1.21 (m, 2H), 1.15 (t, J=7.2 Hz, 3H), 0.94-0.78 (m, 2H), 0.59-0.45 (m, 1H), 0.37-0.23 (m, 1H).
    • Example 101
  • Figure US20200115354A1-20200416-C00324
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-morpholinocyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 70 and making non-critical variations as required to replace piperidine with morpholine, 5-chloro-2-fluoro-4-(((1S,2S)-2-morpholinocyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 475.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.60 (d, J=7.2 Hz, 1H), 7.23 (d, J=4.4 Hz, 1H), 6.79 (d, J=4.4 Hz, 1H), 6.70 (d, J=12.8 Hz, 1H), 5.95 (s, 1H), 3.45-3.40 (m, 4H), 3.28-3.27 (m, 1H), 2.62-2.57 (m, 1H), 2.45-2.27 (m, 4H), 2.20-2.10 (m, 1H), 1.90-1.85 (m, 1H), 1.80-1.70 (m, 1H), 1.65-1.55 (m, 1H), 1.40-1.30 (m, 1H), 1.28-1.09 (m, 3H).
    • Example 102
  • Figure US20200115354A1-20200416-C00325
    • 5-chloro-4-(((1S,2S)-2-(ethyl(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(4-methylthiazol-2-yl)benzenesulfonamide formate Step 1
  • Figure US20200115354A1-20200416-C00326
    • 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(4-methylthiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 29 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(4-methylthiazol-2-yl)benzenesulfonamide, 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(4-methylthiazol-2-yl)benzenesulfonamide was obtained as a white solid.
    • Step 2
  • Figure US20200115354A1-20200416-C00327
    • 5-chloro-4-(((1S,2S)-2-(ethyl(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(4-methylthiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 58 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide and tert-butyl N-methyl-N-(2-oxoethyl)carbamate with 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(4-methylthiazol-2-yl)benzenesulfonamide and acetaldehyde, 5-chloro-4-(((1S,2S)-2-(ethyl(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(4-methylthiazol-2-yl)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 461.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.15 (s, 1H), 7.58 (d, J=7.2 Hz, 1H), 6.72 (d, J=13.2 Hz, 1H), 6.35 (d, J=0.8 Hz, 1H), 5.92 (d, J=3.6 Hz, 1H), 3.28-3.27 (m, 1H), 2.69-2.64 (m, 1H), 2.45-2.41 (m, 2H), 2.14 (s, 3H), 2.08 (s, 3H), 1.87-1.72 (m, 2H), 1.65-1.56 (m, 1H), 1.43-1.33 (m, 1H), 1.29-1.08 (m, 4H), 0.96 (t, J=7.2 Hz, 3H).
    • Example 104
  • Figure US20200115354A1-20200416-C00328
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(3-fluoropyrrolidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 70 and making non-critical variations as required to replace piperidine with 3-fluoropyrrolidine, 5-chloro-2-fluoro-4-(((1S,2S)-2-(3-fluoropyrrolidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 477.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.57 (d, J=6.8 Hz, 1H), 7.24 (d, J=4.0 Hz, 1H), 6.79 (d, J=4.0 Hz, 1H), 6.72 (d, J=12.8 Hz, 1H), 5.84 (s, 1H), 5.24-4.92 (m, 1H), 3.28-3.27 (m, 1H), 2.95-2.85 (m, 1H), 2.82-2.68 (m, 4H), 2.13-2.05 (m, 1H), 1.80-1.40 (m, 4H), 1.65-1.55 (m, 1H), 1.45-1.35 (m, 1H), 1.31-1.14 (m, 3H).
    • Example 105 & Example 106
  • Figure US20200115354A1-20200416-C00329
    • 4-(((1S,2S)-2-(3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide & 4-(((1R,2R)-2-(3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide
  • Following the procedure described in Example 98 & Example 99 and making non-critical variations as required to replace pyrrolidine with 3-azabicyclo[3.1.0]hexane, the title compounds were obtained as a white solid. The stereochemistry was assigned arbitrarily to each enantiomer. Example 105: LCMS (ESI) m/z: 483.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 11.40 (s, 1H), 7.83 (q, J=8.2 Hz, 1H), 7.73 (d, J=7.4 Hz, 1H), 6.88 (dd, J=7.9, 2.1 Hz, 1H), 6.78-6.63 (m, 2H), 5.86 (d, J=5.3 Hz, 1H), 3.28-3.05 (m, 1H), 2.89-2.62 (m, 3H), 2.42-2.29 (m, 1H), 1.88-1.66 (m, 1H), 1.67-1.53 (m, 1H), 1.51-0.99 (m, 6H), 0.37 (q, J=3.8 Hz, 1H), 0.24-0.10 (m, 1H). Example 106: LCMS (ESI) m/z: 483.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 11.40 (s, 1H), 7.83 (q, J=8.2 Hz, 1H), 7.73 (d, J=7.4 Hz, 1H), 6.88 (dd, J=7.9, 2.1 Hz, 1H), 6.78-6.63 (m, 2H), 5.86 (d, J=5.3 Hz, 1H), 3.28-3.05 (m, 1H), 2.89-2.62 (m, 3H), 2.42-2.29 (m, 1H), 1.88-1.66 (m, 1H), 1.67-1.53 (m, 1H), 1.51-0.99 (m, 6H), 0.37 (q, J=3.8 Hz, 1H), 0.24-0.10 (m, 1H).
    • Example 107
  • Figure US20200115354A1-20200416-C00330
    • 5-cyclopropyl-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 15 and making non-critical variations as required to replace 5-bromo-N-[(2,4-dimethoxyphenyl)methyl]-4-[[(1S,2S)-2-(dimethylamino)cyclohexyl]amino]-2-fluoro-N-thiazol-2-yl-benzenesulfonamide with 5-chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(6-fluoropyridin-2-yl)-N-(methoxymethyl)benzenesulfonamide, 5-cyclopropyl-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 451.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.17 (s, 1H), 7.80-7.72 (m, 1H), 7.42 (d, J=8.4 Hz, 1H), 6.84-6.77 (m, 1H), 6.63-6.59 (m, 1H), 6.47 (d, J=14.0 Hz, 1H), 6.09 (s, 1H), 3.25-3.14 (m, 1H), 2.69-2.57 (m, 1H), 2.20 (s, 3H), 2.20 (s, 3H), 2.18-2.12 (m, 1H), 1.88-1.73 (m, 2H), 1.67-1.50 (m, 2H), 1.45-1.31 (m, 1H), 1.27-1.06 (m, 3H), 0.95-0.86 (m, 2H), 0.65-0.56 (m, 1H), 0.32-0.23 (m, 1H).
    • Example 108
  • Figure US20200115354A1-20200416-C00331
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(piperidin-1-yl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 70 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide, 5-chloro-2-fluoro-4-(((1S,2S)-2-(piperidin-1-yl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 468.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.53 (s, 1H), 8.24 (s, 1H), 7.71 (d, J=6.8 Hz, 1H), 6.90 (d, J=4.6 Hz, 1H), 6.75 (d, J=12.8 Hz, 1H), 6.04 (s, 1H), 3.38-3.35 (m, 1H), 2.74-2.66 (m, 1H), 2.64-2.55 (m, 2H), 2.47-2.44 (m, 2H), 2.15-2.05 (m, 1H), 1.95-1.85 (m, 1H), 1.80-1.70 (m, 1H), 1.65-1.55 (m, 1H), 1.52-1.14 (m, 10H).
    • Example 109
  • Figure US20200115354A1-20200416-C00332
    • 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-(methyl(1-methylpiperidin-4-yl)amino)cyclohexyl)amino)benzenesulfonamide
  • Following the procedure described in Example 55 and making non-critical variations as required to replace tert-butyl 3-oxoazetidine-1-carboxylate with 1-methylpiperidin-4-one, 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-(methyl(1-methylpiperidin-4-yl)amino)cyclohexyl)amino)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 528.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.76-7.54 (m, 2H), 6.71 (dd, J=8.0, 2.3 Hz, 1H), 6.64 (d, J=13.1 Hz, 1H), 6.43 (d, J=7.9 Hz, 1H), 5.75 (d, J=4.8 Hz, 1H), 2.99-2.80 (m, 2H), 2.73-2.63 (m, 1H), 2.47-2.36 (m, 1H), 2.35-2.31 (m, 1H), 2.30 (s, 3H), 2.25-2.09 (m, 3H), 2.07 (s, 3H), 1.81-1.67 (m, 3H), 1.66-1.12 (m, 7H).
    • Example 110
  • Figure US20200115354A1-20200416-C00333
    • 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-(methyl(piperidin-4-yl)amino)cyclohexyl)amino)benzenesulfonamide
  • Following the procedure described in Example 55 and making non-critical variations as required to replace tert-butyl 3-oxoazetidine-1-carboxylate with tert-butyl 4-oxopiperidine-1-carboxylate, 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-(methyl(piperidin-4-yl)amino)cyclohexyl)amino)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 528.1 [M+H]+. H NMR (400 MHz, DMSO-d6) δ 7.61 (d, J=7.2 Hz, 1H), 7.39 (q, J=8.4 Hz, 1H), 6.61-6.46 (m, 2H), 6.09 (dd, J=7.8, 3.0 Hz, 1H), 5.51 (d, J=4.1 Hz, 1H), 3.25-3.13 (m, 3H), 2.91-2.74 (m, 2H), 2.74-2.58 (m, 2H), 2.24-2.13 (m, 1H), 2.08 (s, 3H), 1.91-1.81 (m, 1H), 1.79-1.53 (m, 6H), 1.46-1.05 (m, 4H).
    • Example 111
  • Figure US20200115354A1-20200416-C00334
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-((2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-N-(4-methylthiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 29 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide and iodopropane with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(4-methylthiazol-2-yl)benzenesulfonamide and 2-bromoethanol, 5-chloro-2-fluoro-4-(((1S,2S)-2-((2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-N-(4-methylthiazol-2-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 477.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.57 (d, J=7.2 Hz, 1H), 6.70 (d, J=12.8 Hz, 1H), 6.36 (s, 1H), 5.96 (d, J=4.0 Hz, 1H), 4.35-4.25 (m, 1H), 3.28-3.19 (m, 3H), 2.59-2.54 (m, 2H), 2.45-2.37 (m, 2H), 2.17 (s, 3H), 2.08 (s, 3H), 1.83-1.74 (m, 2H), 1.64-1.56 (m, 1H), 1.41-1.34 (m, 1H), 1.28-1.21 (m, 2H), 1.17-1.05 (m, 1H).
    • Example 112
  • Figure US20200115354A1-20200416-C00335
    • 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00336
    • Step 1
  • Figure US20200115354A1-20200416-C00337
    • tert-butyl((1S,6S)-6-(dimethylamino)-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate
  • To a solution of tert-butyl((1S,6S)-6-amino-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate (500 mg, 1.78 mmol) in MeOH (10 mL) was added AcOH (0.2 mL, 3.57 mmol) and paraformaldehyde (268 mg, 8.92 mmol). The mixture was stirred at room temperature for 10 min and sodium cyanoborohydride (560 mg, 8.92 mmol) was added. The mixture was stirred at room temperature for an additional 16 h. The reaction was quenched with sat. aq. NaHCO3 (10 mL) and extracted with DCM (20 mL×3). The combined organic layer were dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (solvent gradient: 0-100% EtOAc (0.5% TEA) in petroleum ether) to give the title compound (230 mg, 42%) as a light yellow solid.
    • Step 2
  • Figure US20200115354A1-20200416-C00338
    • tert-butyl((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)carbamate
  • To a solution of tert-butyl((1S,6S)-6-(dimethylamino)-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate (230 mg, 0.75 mmol) in EtOAc (20 mL) was added Pd(OH)2 (20%, 524 mg, 0.75 mmol). The mixture was stirred at room temperature for 2 h under hydrogen atmosphere (15 psi). The mixture was filtered and concentrated in vacuo. The crude residue was purified by Prep-TLC (petroleum ether/EtOAc=3:1) to give the title compound (200 mg, 86%) as colorless oil. 1H NMR (400 MHz, CDCl3) δ 5.31 (s, 1H), 4.28-3.14 (m, 1H), 2.69-2.56 (m, 1H), 2.37-2.30 (m, 1H), 2.25 (s, 3H), 2.25 (s, 3H), 2.05-2.00 (m, 1H), 1.94-1.87 (m, 1H), 1.49-1.40 (m, 1H), 1.46 (s, 9H), 1.40-1.34 (m, 1H), 1.29-1.22 (m, 1H), 1.18-1.06 (m, 1H).
    • Step 3
  • Figure US20200115354A1-20200416-C00339
    • (1S,2S,5S)—N1,N1-dimethyl-5-(trifluoromethyl)cyclohexane-1,2-diamine
  • To a solution of tert-butyl((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)carbamate (100 mg, 0.32 mmol) in DCM (1 mL) was added trifluoroacetic acid (0.48 mL, 6.44 mmol). The mixture was stirred at room temperature for 2 h and concentrated in vacuo to give the title compound (67 mg, crude) as light yellow oil that required no further purification.
    • Step 4
  • Figure US20200115354A1-20200416-C00340
    • 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • To a solution of 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide (436 mg, 0.96 mmol) and N,N-diisopropylethylamine (0.53 mL, 3.19 mmol) in DMF (5 mL) was added (1S,2S,5S)—N1,N1-dimethyl-5-(trifluoromethyl)cyclohexane-1,2-diamine (67 mg, 0.32 mmol). The reaction mixture was stirred at room temperature for 16 h and concentrated in vacuo. The crude residue was purified by silica gel chromatography (solvent gradient: 0-10%-25% EtOAc (1% TEA) in petroleum ether) to give the title compound (120 mg, 58%) as a light yellow solid. LCMS (ESI) m/z: 646.3 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 8.80 (s, 1H), 8.45 (d, J=6.0 Hz, 1H), 7.88 (d, J=7.2 Hz, 1H), 7.31 (d, J=6.0 Hz, 1H), 7.20 (d, J=8.0 Hz, 1H), 6.44-6.36 (m, 2H), 6.25 (d, J=12.4 Hz, 1H), 5.97 (s, 1H), 5.27 (s, 2H), 3.81 (s, 3H), 3.77 (s, 3H), 3.05-2.98 (m, 1H), 2.65-2.55 (m, 1H), 2.46-2.35 (m, 1H), 2.30-2.10 (m, 4H), 2.25 (s, 3H), 2.05-1.95 (m, 1H), 1.52-1.41 (m, 1H), 1.38-1.28 (m, 1H), 1.25-1.16 (m, 1H).
    • Step 5
  • Figure US20200115354A1-20200416-C00341
    • 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(trifluromethyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • A mixture of 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (120 mg, 0.19 mmol) and formic acid (5.0 mL) was stirred at room temperature for 1 h. The reaction mixture was concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 5-35%/0.225% formic acid in water) to give the title compound (50 mg, 54%) as a white solid. LCMS (ESI) m/z: 496.3 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.51 (s, 1H), 8.20 (d, J=6.0 Hz, 1H), 7.68 (d, J=7.2 Hz, 1H), 6.92-6.76 (m, 2H), 5.95 (d, J=6.4 Hz, 1H), 3.67-3.53 (m, 1H), 3.15-3.00 (m, 1H), 2.45-2.39 (m, 1H), 2.34 (s, 3H), 2.34 (s, 3H), 2.15-2.02 (m, 2H), 1.85-1.73 (m, 1H), 1.59-1.45 (m, 1H), 1.43-1.20 (m, 2H).
    • Example 113
  • Figure US20200115354A1-20200416-C00342
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(4-fluoropiperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 70 and making non-critical variations as required to replace piperidine with 4-fluoropiperidine, 5-chloro-2-fluoro-4-(((1S,2S)-2-(4-fluoropiperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 490.9 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.56 (d, J=7.2 Hz, 1H), 7.15 (s, 1H), 6.77-6.57 (m, 2H), 5.87 (d, J=3.2 Hz, 1H), 4.61-4.47 (m, 1H), 3.11-3.03 (m, 1H), 2.67-2.50 (m, 3H), 2.44-2.34 (m, 2H), 2.27-2.18 (m, 1H), 2.15-2.08 (m, 1H), 1.80-1.68 (m, 2H), 1.63-1.52 (m, 3H), 1.50-1.41 (m, 1H), 1.39-1.27 (m, 1H), 1.20-1.10 (m, 3H).
    • Example 114
  • Figure US20200115354A1-20200416-C00343
    • 5-Cyclopropyl-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)oxy)-2-fluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 66 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide and tert-butyl 3-(((1S,2S)-2-hydroxycyclohexyl)(methyl)amino)azetidine-1-carboxylate with N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)-5-(cyclopropyl)benzenesulfonamide and (1S,2S)-2-(dimethylamino)cyclohexanol, the title compound was obtained as a white solid. LCMS (ESI) m/z: 452.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 11.94-10.89 (m, 1H), 8.14 (s, 1H), 7.72 (s, 1H), 7.30 (d, J=8.4 Hz, 1H), 7.13 (d, J=12.6 Hz, 1H), 6.80-6.70 (m, 1H), 6.62-6.46 (m, 1H), 4.70-4.48 (m, 1H), 2.38-2.19 (m, 7H), 2.18-1.94 (m, 2H), 1.89-1.74 (m, 1H), 1.73-1.52 (m, 2H), 1.45-1.14 (m, 4H), 1.00-0.80 (m, 2H), 0.71-0.51 (m, 2H).
    • Example 115
  • Figure US20200115354A1-20200416-C00344
    • 5-Chloro-4-(((1S,2S)-2-(cyclobutyl(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 18 and making non-critical variations as required to replace acetaldehyde with cyclobutanone, the title compound was obtained as a white solid. LCMS (ESI) m/z: 473.0, 474.9 [M+H]+. 1H NMR (400 MHz, dmso) δ 12.89-12.18 (m, 2H), 8.14 (s, 1H), 7.59 (d, J=7.3 Hz, 1H), 7.20 (d, J=4.0 Hz, 1H), 6.74 (d, J=3.8 Hz, 1H), 6.69 (d, J=12.8 Hz, 1H), 5.82 (d, J=3.5 Hz, 1H), 3.25-3.09 (m, 2H), 2.17 (d, J=10.4 Hz, 1H), 2.00-1.87 (m, 5H), 1.80-1.65 (m, 4H), 1.65-1.48 (m, 4H), 1.44-1.30 (m, 1H), 1.29-1.17 (m, 2H), 1.17-1.05 (m, 1H).
    • Example 116
  • Figure US20200115354A1-20200416-C00345
    • 5-Chloro-2-fluoro-4-(((1S,2S)-2-((2-hydroxy-2-methylpropyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide formate
  • Figure US20200115354A1-20200416-C00346
    • Step 1
  • Figure US20200115354A1-20200416-C00347
    • 5-Chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-((2-hydroxy-2-methylpropyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • To a mixture of 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (150 mg, 0.26 mmol) and lithium perchlorate (280 mg, 2.64 mmol), in THF (1 mL), was added a solution of 1,2-Epoxy-2-methylpropane (28 mg, 0.40 mmol), in Et2O (0.5 mL). After 16 h at rt, a further portion of 1,2-Epoxy-2-methylpropane (28 mg, 0.40 mmol) was added and after 40 h at rt the mixture was diluted with EtOAc (50 mL) and washed with H2O (10 mL), dried (Na2SO4), filtered and concentrated in vacuo. The crude material was purified by flash chromatrography through Si gel (0-100% EtOAc/CH2Cl2) to provide 5-Chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-((2-hydroxy-2-methylpropyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (68 mg, 41%). LCMS (ESI) m/z: 641.1 (M+H)+.
    • Step 2
  • Figure US20200115354A1-20200416-C00348
    • 5-Chloro-2-fluoro-4-(((1S,2S)-2-((2-hydroxy-2-methylpropyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide Formate
  • 5-Chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-((2-hydroxy-2-methylpropyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (68 mg, 0.11 mmol) was treated with 95% formic acid (2 mL), sonicated for 1 min and stirred 1 h at rt. The volatiles were evaporated in vacuo and the residue was purified by C18 reverse phase flash chromatography (0-40% MeCN/10 mM aqueous NH4CO2H, pH=3.8). Appropriate fractions were combined and lyophilized to provide 5-Chloro-2-fluoro-4-(((1S,2S)-2-((2-hydroxy-2-methylpropyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide formate (23 mg, 39%). LCMS (ESI) m/z: 490.1 (M+H)+. 1H NMR (400 MHz, d6-dmso) δ 12.74 (s, 1H), 8.14 (s, 1H), 7.56 (d, J=7.3 Hz, 1H), 7.21 (d, J=4.4 Hz, 1H), 6.76 (d, J=4.3 Hz, 1H), 6.65 (d, J=13.0 Hz, 1H), 6.12 (d, J=2.3 Hz, 1H), 4.15 (s, 1H), 3.27-3.15 (m, 1H), 2.62 (t, J=11.5 Hz, 1H), 2.40 (d, J=13.7 Hz, 1H), 2.22 (d, J=13.7 Hz, 1H), 2.16 (s, 3H), 2.15-2.11 (m, 1H), 1.76 (t, J=10.5 Hz, 2H), 1.58 (d, J=13.0 Hz, 1H), 1.46-1.11 (m, 4H), 1.05 (s, 3H), 1.03 (s, 3H).
    • Example 117 & Example 123
  • Figure US20200115354A1-20200416-C00349
    • 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1R,2R)-2-(piperidin-1-yl)cyclohexyl)amino)benzenesulfonamide & 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-(piperidin-1-yl)cyclohexyl)amino)benzenesulfonamide
  • Following the procedure described in Example 98 & Example 99 and making non-critical variations as required to replace pyrrolidine with piperidine, the title compounds were obtained as a white solid. Example 117: LCMS (ESI) m/z: 485.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 11.37 (s, 1H), 7.80 (q, J=8.2 Hz, 1H), 7.74 (d, J=7.4 Hz, 1H), 6.85 (dd, J=7.9, 2.2 Hz, 1H), 6.73 (d, J=13.3 Hz, 1H), 6.66 (d, J=8.1 Hz, 1H), 6.10 (d, J=4.5 Hz, 1H), 3.39-3.32 (m, 1H), 3.29-3.21 (m, 1H), 2.47-2.39 (m, 1H), 2.37-2.24 (m, 2H), 2.19-2.08 (m, 1H), 1.88-1.68 (m, 2H), 1.66-1.55 (m, 1H), 1.45-1.09 (m, 10H). Example 123: LCMS (ESI) m/z: 485.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 11.37 (s, 1H), 7.81 (q, J=8.2 Hz, 1H), 7.74 (d, J=7.4 Hz, 1H), 6.86 (dd, J=8.0, 2.1 Hz, 1H), 6.70 (dd, J=25.2, 10.6 Hz, 2H), 6.10 (d, J=4.9 Hz, 1H), 3.29-3.18 (m, 1H), 2.40-2.18 (m, 1H), 2.19-2.05 (m, 1H), 1.91-1.67 (m, 2H), 1.68-1.55 (m, 1H), 1.47-1.08 (m, 11H).
    • Example 118
  • Figure US20200115354A1-20200416-C00350
    • 5-Chloro-2-fluoro-4-(((1S,2S)-2-(((S)-3-fluoro-2-hydroxypropyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 116 and making non-critical variations required to replace 1,2-Epoxy-2-methylpropane with epifluorohydrin, the title compound was obtained as a mixture of disatereomers. Separation of isomers by HPLC provided the title compound. The stereochemistry of the alcohol stereocenter was assigned arbitrarily. LCMS (ESI) m/z: 495.0 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 12.69 (s, 1H), 7.58 (d, J=7.3 Hz, 1H), 7.27 (d, J=4.6 Hz, 1H), 6.82 (d, J=4.6 Hz, 1H), 6.75 (d, J=13.1 Hz, 1H), 5.88 (d, J=5.3 Hz, 1H), 4.99 (s, 1H), 4.41 (ddd, J=26.3, 9.5, 4.2 Hz, 1H), 4.29 (ddd, J=26.4, 9.5, 4.3 Hz, 1H), 3.87-3.67 (m, 1H), 2.86-2.53 (m, 3H), 2.17 (s, 3H), 2.08 (d, J=12.5 Hz, 1H), 1.77 (dd, J=17.5, 10.9 Hz, 2H), 1.59 (d, J=13.0 Hz, 1H), 1.46-1.04 (m, 5H).
    • Example 119
  • Figure US20200115354A1-20200416-C00351
    • 5-Chloro-2-fluoro-4-(((1S,2S)-2-(((R)-3-fluoro-2-hydroxypropyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 116 and making non-critical variations required to replace 1,2-Epoxy-2-methylpropane with epifluorohydrin, the title compound was obtained as a mixture of disatereomers. Separation of isomers by HPLC provided the title compound. The stereochemistry of the alcohol stereocenter was assigned arbitrarily. LCMS (ESI) m/z: 495.0 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 12.69 (s, 1H), 7.57 (d, J=7.3 Hz, 1H), 7.22 (d, J=4.5 Hz, 1H), 6.77 (d, J=4.5 Hz, 1H), 6.69 (d, J=13.0 Hz, 1H), 5.78 (d, J=4.9 Hz, 1H), 4.85 (s, 1H), 4.30 (ddd, J=48.0, 9.4, 2.7 Hz, 1H), 4.09 (ddd, J=48.2, 9.5, 5.5 Hz, 1H), 3.65-3.55 (m, 1H), 2.59 (t, J=9.2 Hz, 1H), 2.41 (dd, J=12.9, 7.9 Hz, 1H), 2.34 (dd, J=10.1, 2.5 Hz, 1H), 2.17 (s, 3H), 2.08 (dd, J=11.5, 5.7 Hz, 1H), 1.81 (d, J=9.7 Hz, 1H), 1.74 (d, J=9.8 Hz, 1H), 1.59 (d, J=12.1 Hz, 1H), 1.43-1.05 (m, 5H).
    • Example 120 & Example 121
  • Figure US20200115354A1-20200416-C00352
    • 5-chloro-4-(((1S,2S,5R)-2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide & 5-chloro-4-(((1S,2S,5S)-2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 88 and making non-critical variations as required to replace (trans)-tert-butyl(3-amino-3′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate and 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide with tert-butyl((3S,4S)-3-amino-3′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate and 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide, 5-chloro-4-(((1S,2S,5R)-2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (first peak on HPLC) and 5-chloro-4-(((1S,2S,5S)-2-(dimethylamino)-5-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (second peak on HPLC) were obtained both as white solid. Example 120: LCMS (ESI) m/z: 572.3 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.40 (s, 1H), 8.13 (s, 1H), 8.09 (d, J=5.6 Hz, 1H), 7.70-7.66 (m, 3H), 7.60-7.58 (m, 2H), 6.72 (d, J=5.6 Hz, 1H), 6.38 (d, J=12.0 Hz, 1H), 5.69 (d, J=6.8 Hz, 1H), 3.60-3.58 (m, 1H), 3.17-3.16 (m, 1H), 2.73-2.71 (m, 1H), 2.35-2.34 (m, 1H), 2.34 (s, 3H), 2.34 (s, 3H), 2.09-2.06 (m, 1H), 1.81-1.68 (m, 4H). Example 119: LCMS (ESI) m/z: 572.3 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.40 (s, 1H), 8.14 (s, 1H), 8.07 (d, J=6.0 Hz, 1H), 7.64 (d, J=7.2 Hz, 1H), 7.54-7.53 (m, 4H), 6.88 (d, J=12.8 Hz, 1H), 6.72 (d, J=6.0 Hz, 1H), 5.90 (d, J=7.2 Hz, 1H), 3.76-3.73 (m, 1H), 2.95-2.94 (m, 1H), 2.44 (s, 3H), 2.44 (s, 3H), 2.39-2.28 (m, 1H), 2.17-2.13 (m, 1H), 2.05-2.04 (m, 1H), 2.00-1.98 (m, 1H), 1.58-1.50 (m, 3H).
    • Example 122
  • Figure US20200115354A1-20200416-C00353
    • 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-((3-hydroxypropyl)(methyl)amino)cyclohexyl)amino)benzenesulfonamide
  • Following the procedure described in Example 72 and making non-critical variations as required to replace 2-bromoethanol with 3-iodopropanol, 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-((3-hydroxypropyl)(methyl)amino)cyclohexyl)amino)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 489.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.84-7.74 (m, 1H), 7.72 (d, J=7.4 Hz, 1H), 6.83 (d, J=7.9 Hz, 1H), 6.74 (d, J=13.2 Hz, 1H), 6.69-6.54 (m, 1H), 6.00 (s, 1H), 3.48-3.33 (m, 4H), 3.29-3.22 (m, 1H), 2.20-2.08 (m, 4H), 1.89-1.69 (m, 2H), 1.67-1.44 (m, 3H), 1.44-1.06 (m, 4H).
    • Example 124
  • Figure US20200115354A1-20200416-C00354
    • 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-((3-hydroxy-3-methylbutyl)(methyl)amino)cyclohexyl)amino)benzenesulfonamide
  • Following the procedure described in Example 72 and making non-critical variations as required to replace 2-bromoethanol with 4-iodo-2-methylbutan-2-ol, 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-((3-hydroxy-3-methylbutyl)(methyl)amino)cyclohexyl)-amino)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 517.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.67 (t, J=6.9 Hz, 2H), 6.78-6.63 (m, 2H), 6.61-6.39 (m, 3H), 5.90 (s, 1H), 2.74-2.69 (m, 1H), 2.48-2.41 (m, 2H), 2.15 (s, 3H), 2.11-2.02 (m, 1H), 1.90-1.69 (m, 2H), 1.67-1.47 (m, 2H), 1.45-1.09 (m, 6H), 0.97 (s, 6H).
    • Example 125
  • Figure US20200115354A1-20200416-C00355
    • 5-Chloro-4-(((1S,2S)-2-((2-cyanoethyl)(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 29 and making non-critical variations as required to replace the iodopropane with acrylonitrile, the title compound was obtained as a white solid. LCMS (ESI) m/z: 471.9 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 12.61 (s, 1H), 7.55 (d, J=7.4 Hz, 1H), 7.24 (d, J=4.6 Hz, 1H), 6.79 (d, J=4.6 Hz, 1H), 6.70 (d, J=13.1 Hz, 1H), 5.82 (d, J=4.2 Hz, 1H), 3.32-3.28 (m, 1H), 2.73-2.59 (m, 3H), 2.59-2.51 (m, 2H), 2.09 (s, 3H), 2.09-2.04 (m, J=2.9 Hz, 1H), 1.79-1.67 (m, 2H), 1.58 (d, J=12.8 Hz, 1H), 1.40-1.04 (m, 4H).
    • Example 126
  • Figure US20200115354A1-20200416-C00356
    • 5-Chloro-4-(((1S,2S)-2-(((S)-3,3-difluoro-2-hydroxypropyl)(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 116 and making non-critical variations required to replace 1,2-Epoxy-2-methylpropane with 2-(difluoromethyl)oxirane, the title compound was obtained as a mixture of disatereomers. Separation of isomers by HPLC provided the title compound. The stereochemistry of the alcohol stereocenter was assigned arbitrarily. LCMS (ESI) m/z: 513.1 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 12.52 (br s, 1H), 7.57 (d, J=7.3 Hz, 1H), 7.23 (d, J=4.4 Hz, 1H), 6.78 (d, J=4.4 Hz, 1H), 6.70 (d, J=13.0 Hz, 1H), 6.01-5.64 (m, 2H), 3.76-3.61 (m, 1H), 2.68-2.58 (m, 1H), 2.58-2.53 (m, 2H), 2.14 (s, 3H), 2.09 (d, J=11.4 Hz, 1H), 1.76 (t, J=11.6 Hz, 2H), 1.59 (d, J=12.3 Hz, 1H), 1.43-1.00 (m, 4H). 2 H not visible (under the HOD signal).
    • Example 127
  • Figure US20200115354A1-20200416-C00357
    • 5-Chloro-4-(((1S,2S)-2-(((R)-3,3-difluoro-2-hydroxypropyl)(methyl)amino)-cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 116 and making non-critical variations required to replace 1,2-Epoxy-2-methylpropane with 2-(difluoromethyl)oxirane, the title compound was obtained as a mixture of disatereomers. Separation of isomers by HPLC provided the title compound. The stereochemistry of the alcohol stereocenter was assigned arbitrarily. LCMS (ESI) m/z: 513.2 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 12.69 (br s, 1H), 7.57 (d, J=7.3 Hz, 1H), 7.24 (d, J=4.5 Hz, 1H), 6.79 (d, J=4.5 Hz, 1H), 6.70 (d, J=13.1 Hz, 1H), 5.86 (d, J=4.6 Hz, 1H), 5.75 (td, J=55.6, 2.5 Hz, 1H), 5.31 (br s, 1H), 3.77-3.64 (m, 1H), 2.63 (td, J=10.8, 2.9 Hz, 1H), 2.55 (dd, J=13.1, 6.5 Hz, 1H), 2.45 (dd, J=13.0, 6.8 Hz, 1H), 2.16 (s, 3H), 2.07 (d, J=10.7 Hz, 1H), 1.80 (d, J=10.6 Hz, 1H), 1.74 (d, J=9.2 Hz, 1H), 1.59 (d, J=12.8 Hz, 1H), 1.40-1.29 (m, 1H), 1.28-1.19 (m, 2H), 1.18-1.06 (m, 1H). 1 H under HOD signal.
    • Example 128
  • Figure US20200115354A1-20200416-C00358
    • 5-Cyclopropyl-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S)-2-((2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)benzenesulfonamide Formate
  • Following the procedure described in Example 72 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(6-fluoropyridine-2-yl)benzenesulfonamide with 5-(cyclopropyl)-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(6-fluoropyridine-2-yl)benzenesulfonamide, the title compound was obtained as a white solid. LCMS (ESI) m/z: 481.2 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 11.27 (br. s, 1H), 8.14 (s, 1H), 7.97-7.61 (m, 1H), 7.40 (d, J=8.3 Hz, 1H), 6.82 (d, J=7.6 Hz, 1H), 6.70-6.57 (m, 1H), 6.43 (d, J=13.7 Hz, 1H), 6.13 (s, 1H), 4.46-4.27 (m, 1H), 3.48-3.39 (m, 3H), 3.22-3.10 (m, 1H), 2.19 (dd, J=21.2, 11.1 Hz, 1H), 2.10 (s, 3H), 1.87-1.71 (m, 2H), 1.67-1.48 (m, 3H), 1.47-1.15 (m, 4H), 1.08 (dd, J=23.3, 10.3 Hz, 1H), 0.99-0.82 (m, 2H), 0.67-0.52 (m, 1H), 0.38-0.23 (m, 1H).
    • Example 129
  • Figure US20200115354A1-20200416-C00359
    • 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate Step 1
  • Figure US20200115354A1-20200416-C00360
    • tert-butyl((1S,2S,4R)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)carbamate & tert-butyl((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)carbamate
  • Following the procedure described in Example 112 and making non-critical variations as required to replace tert-butyl((1S,6S)-6-amino-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate with tert-butyl((3S,4S)-3-amino-3′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate, tert-butyl((1S,2S,4R)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)carbamate (20 mg, less polar on TLC) as a light yellow solid and tert-butyl((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)carbamate (80 mg, more polar on TLC) as a light yellow solid. Less polar on TLC: 1H NMR (400 MHz, CDCl3) δ 7.50-7.27 (m, 4H), 5.56-5.45 (m, 1H), 3.43-3.41 (m, 1H), 2.72-2.64 (m, 2H), 2.58-2.57 (m, 1H), 2.36 (s, 3H), 2.36 (s, 3H), 2.06-2.01 (m, 1H), 1.93-1.89 (m, 1H), 1.47 (s, 9H). More polar on TLC: 1H NMR (400 MHz, DMSO-d6) δ 7.62-7.58 (m, 2H), 7.54-7.48 (m, 2H), 6.39-6.35 (m, 1H), 3.40-3.37 (m, 1H), 2.67-2.63 (m, 1H), 2.19 (s, 3H), 2.19 (s, 3H), 2.03-2.01 (m, 1H), 1.85-1.81 (m, 1H), 1.68-1.63 (m, 1H), 1.53-1.42 (m, 2H), 1.38 (s, 9H).
    • Step 2
  • Figure US20200115354A1-20200416-C00361
    • 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 112 and making non-critical variations as required to replace tert-butyl((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)-carbamate and 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide with tert-butyl((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)carbamate and 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide, 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate was obtained as a light yellow solid. LCMS (ESI) m/z: 577.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.14 (s, 1H), 7.65-7.54 (m, 5H), 7.22 (d, J=4.4 Hz, 1H), 6.82 (d, J=13.2 Hz, 1H), 6.77 (d, J=4.4 Hz, 1H), 5.99 (s, 1H), 3.61-3.57 (m, 1H), 3.08-3.07 (m, 1H), 2.85-2.81 (m, 1H), 2.32 (s, 3H), 2.32 (s, 3H), 2.17-2.13 (m, 1H), 2.05-2.01 (m, 1H), 1.78-1.73 (m, 2H), 1.64-1.56 (m, 1H), 1.39-1.37 (m, 1H).
    • Example 130
  • Figure US20200115354A1-20200416-C00362
    • 5-chloro-4-(((1S,6S)-6-(dimethylamino)-3-(trifluoromethyl)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 48 and making non-critical variations as required to replace tert-butyl((1R,6R)-6-amino-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate with tert-butyl((1S,6S)-6-amino-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate, 4-(((1S,6S)-6-amino-3-(trifluoromethyl)cyclohex-3-en-1-yl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 499.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.58 (d, J=7.2 Hz, 1H), 7.24 (d, J=4.4 Hz, 1H), 6.89 (d, J=12.8 Hz, 1H), 6.79 (d, J=4.4 Hz, 1H), 6.44 (s, 1H), 6.09 (d, J=6.0 Hz, 1H), 3.88-3.77 (m, 1H), 3.17-3.09 (m, 1H), 2.71-2.61 (m, 2H), 2.31-2.27 (m, 1H), 2.23 (s, 3H), 2.23 (s, 3H), 2.17-2.06 (m, 1H).
    • Example 131
  • Figure US20200115354A1-20200416-C00363
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(4-hydroxypiperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 70 and making non-critical variations as required to replace piperidine with 4-hydroxypiperidine, the title compound was obtained as a white solid. LCMS (ESI) m/z: 489.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.66 (s, 1H), 7.60 (d, J=7.2 Hz, 1H), 7.24 (d, J=4.5 Hz, 1H), 6.79 (d, J=4.5 Hz, 1H), 6.68 (d, J=12.7 Hz, 1H), 6.03 (dd, J=3.7, 1.7 Hz, 1H), 4.49 (s, 1H), 3.42-3.21 (m, 1H), 2.64-2.50 (m, 3H), 2.47-2.37 (m, 1H), 2.23-2.05 (m, 2H), 1.83 (d, J=8.0 Hz, 1H), 1.75 (s, 1H), 1.62 (d, J=13.2 Hz, 3H), 1.37 (t, J=10.5 Hz, 2H), 1.27-1.07 (m, 4H).
    • Example 132
  • Figure US20200115354A1-20200416-C00364
    • 4-((1S,2S)-2-(dimethylamino)cyclohexyl)-6-fluoro-N-(thiazol-2-yl)-3,4-dihydro-2H-benzo[b][1,4]oxazine-7-sulfonamide
  • Following the procedure described in Example 54 and making non-critical variations as required to replace tert-butyl 2-(4-bromo-2-chlorophenoxy)acetate with 2-(4-bromo-2,5-difluorophenoxy)acetic acid the title compound was obtained as a white solid. LCMS (ESI) m/z: 441.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.30 (s, 1H), 7.26 (d, J=4.6 Hz, 1H), 7.18 (d, J=13.3 Hz, 1H), 7.07 (d, J=7.1 Hz, 1H), 6.82 (d, J=4.6 Hz, 1H), 4.42-4.33 (m, 1H), 4.22-4.13 (m, 1H), 4.11-4.04 (m, 1H), 3.56 (d, J=11.8 Hz, 1H), 3.27-3.18 (m, 2H), 2.79 (s, 3H), 2.66 (s, 3H), 2.10 (d, J=11.8 Hz, 1H), 1.82 (d, J=13.0 Hz, 1H), 1.68 (t, J=12.0 Hz, 2H), 1.61-1.35 (m, 3H), 1.32-1.18 (m, 1H).
    • Example 133
  • Figure US20200115354A1-20200416-C00365
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-((R)-2-(hydroxymethyl)pyrrolidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 70 and making non-critical variations as required to replace the piperidine with (R)-pyrrolidin-2-ylmethanol, the title compound was obtained. LCMS (ESI) m/z: 489.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.63 (s, 1H), 7.57 (d, J=7.3 Hz, 1H), 7.23 (d, J=4.5 Hz, 1H), 6.79 (d, J=4.5 Hz, 1H), 6.67 (d, J=12.9 Hz, 1H), 6.07-6.00 (m, 1H), 4.50 (s, 1H), 3.40 (dd, J=10.3, 5.3 Hz, 1H), 3.32-3.18 (m, 1H), 3.17 (s, 1H), 2.93-2.85 (m, 2H), 2.58 (d, J=8.6 Hz, 1H), 2.44 (s, 1H), 2.16 (d, J=12.4 Hz, 1H), 1.84-1.30 (m, 6H), 1.31-1.05 (m, 2H).
    • Example 134
  • Figure US20200115354A1-20200416-C00366
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-((S)-2-(hydroxymethyl)pyrrolidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 70 and making non-critical variations as required to replace the piperidine with (S)-pyrrolidin-2-ylmethanol, the title compound was obtained. LCMS (ESI) m/z: 489.1 [M+H]+. H NMR (400 MHz, DMSO-d6) δ 7.59 (d, J=7.3 Hz, 1H), 7.21 (d, J=4.5 Hz, 1H), 6.80-6.67 (m, 2H), 5.92 (d, J=6.0 Hz, 1H), 4.41 (s, 1H), 3.29 (s, 2H), 3.13 (s, 1H), 3.07-2.91 (m, 2H), 2.92 (s, 2H), 2.73-2.66 (m, 1H), 2.06 (d, J=9.4 Hz, 1H), 1.83 (d, J=12.4 Hz, 1H), 1.75 (d, J=12.5 Hz, 1H), 1.65-1.50 (m, 5H), 1.47-1.29 (m, 2H), 1.25 (ddd, J=12.6, 9.5, 2.7 Hz, 1H), 1.13 (dt, J=12.6, 9.2 Hz, 1H).
    • Example 135
  • Figure US20200115354A1-20200416-C00367
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-((3-fluoropropyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 72 and making non-critical variations as required to replace 5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2-fluoro-N-(6-fluoro-2-pyridyl)-4-[[(1S,2S)-2-[2-hydroxyethyl(methyl)amino]cyclohexyl]amino]benzenesulfonamide and 2-bromoethanol, with 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide and 1-fluoro-3-iodopropane, 5-chloro-2-fluoro-4-(((1S,2S)-2-((3-fluoropropyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 479.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.59 (s, 1H), 7.59 (d, J=7.3 Hz, 1H), 7.24 (d, J=4.6 Hz, 1H), 6.80 (d, J=4.6 Hz, 1H), 6.71 (d, J=12.9 Hz, 1H), 5.81 (dd, J=5.1, 1.7 Hz, 1H), 4.67-4.25 (m, 2H), 2.72-2.58 (m, 1H), 2.48-2.36 (m, 1H), 2.14-2.09 (m, 5H), 1.88-1.65 (m, 4H), 1.65-1.55 (m, 1H), 1.46-1.07 (m, 4H).
    • Example 136
  • Figure US20200115354A1-20200416-C00368
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(((1-fluorocyclopropyl)methyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 18 and making non-critical variations as required to replace acetaldehyde with 1-fluorocyclopropane carbaldehyde, 5-chloro-2-fluoro-4-(((1S,2S)-2-(((1-fluorocyclopropyl)methyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 491.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.76 (s, 1H), 7.59 (d, J=7.3 Hz, 1H), 7.26 (d, J=4.6 Hz, 1H), 6.82 (d, J=4.6 Hz, 1H), 6.71 (d, J=12.8 Hz, 1H), 5.93 (s, 1H), 2.96-2.64 (m, 2H), 2.24 (s, 3H), 2.20-2.08 (m, 2H), 1.94-1.71 (m, 2H), 1.69-1.57 (m, 1H), 1.48-1.04 (m, 4H), 1.01-0.81 (m, 2H), 0.75-0.43 (m, 2H).
    • Example 137
  • Figure US20200115354A1-20200416-C00369
    • 5-chloro-4-(((1S,2S,4R)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 129 and making non-critical variations as required to replace tert-butyl((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)carbamate with tert-butyl((1S,2S,4R)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)carbamate, 5-chloro-4-(((1S,2S,4R)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide was obtained as a light yellow solid. LCMS (ESI) m/z: 577.3 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.73 (d, J=7.2 Hz, 1H), 7.70-7.66 (m, 2H), 7.60-7.53 (m, 2H), 7.11 (d, J=4.4 Hz, 1H), 6.88 (d, J=12.8 Hz, 1H), 6.73 (d, J=4.4 Hz, 1H), 4.63 (s, 1H), 3.92-3.88 (m, 1H), 3.42-3.98 (m, 1H), 3.11-2.99 (m, 1H), 2.60 (s, 3H), 2.60 (s, 3H), 2.57-2.50 (m, 1H), 2.32-2.24 (m, 1H), 2.11-1.98 (m, 3H), 1.58-1.47 (m, 1H).
    • Example 138
  • Figure US20200115354A1-20200416-C00370
    • 5-chloro-2-fluoro-4-(((1S,2S,4S)-2-((2-hydroxyethyl)(methyl)amino)-4-(trifluoromethyl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00371
    • Step 1
  • Figure US20200115354A1-20200416-C00372
    • tert-butyl((1S,6S)-6-((2-((tert-butyldimethylsilyl)oxy)ethyl)amino)-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate
  • To a solution of tert-butyl((1S,6S)-6-amino-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate (200 mg, 0.71 mmol) in MeOH (5 mL) was added AcOH (0.08 mL, 1.43 mmol) and (tert-butyldimethylsilyloxy)acetaldehyde (187 mg, 1.07 mmol). The mixture was stirred at room temperature for 10 min and sodium cyanoborohydride (135 mg, 2.14 mmol) was added. The mixture was heated to 40° C. for an additional 2 h. After cooling to room temperature, the reaction was quenched with sat. aq. NaHCO3 (50 mL). The mixture was extracted with DCM (50 mL×3). The combined organic layers were dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (solvent gradient: 0-25% EtOAc (0.5% TEA) in petroleum ether) to give the title compound (150 mg, 48%) as a light yellow solid. 1H NMR (400 MHz, CDCl3) δ 6.24 (s, 1H), 5.08-4.72 (m, 1H), 3.78-3.60 (m, 3H), 2.99-2.74 (m, 3H), 2.71-2.63 (m, 1H), 2.62-2.50 (m, 1H), 2.12-1.97 (m, 2H), 1.46 (s, 9H), 0.90 (s, 9H), 0.07 (s, 6H).
    • Step 2
  • Figure US20200115354A1-20200416-C00373
    • tert-butyl((1S,6S)-6-((2-((tert-butyldimethylsilyl)oxy)ethyl)(methyl)amino)-4-(trifluoromethyl) cyclohex-3-en-1-yl)carbamate
  • To a solution of tert-butyl((1S,6S)-6-((2-((tert-butyldimethylsilyl)oxy)ethyl)amino)-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate (150 mg, 0.34 mmol) in MeOH (5 mL) was added AcOH (0.04 mL, 0.68 mmol) and paraformaldehyde (51 mg, 1.71 mmol). The mixture was stirred at room temperature for 10 min and sodium cyanoborohydride (107 mg, 1.71 mmol) was added. The mixture was heated to 40° C. for an additional 4 h. After cooling to room temperature, TEA (0.2 mL) was added and concentrated in vacuo. Water (20 mL) was added and extracted with DCM (50 mL×3). The combined organic layers were dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (solvent gradient: 0-5% EtOAc in petroleum ether) to give the title compound (150 mg, 97%) as a light yellow solid. 1H NMR (400 MHz, CDCl3) δ 6.21 (s, 1H), 5.68 (s, 1H), 3.76-3.63 (m, 2H), 3.54-3.37 (m, 1H), 3.23-3.09 (m, 1H), 2.84-2.73 (m, 1H), 2.66-2.52 (m, 2H), 2.35-2.15 (m, 1H), 2.27 (s, 3H), 2.05-1.90 (m, 1H), 1.65-1.54 (m, 1H), 1.46 (s, 9H), 0.90 (s, 9H), 0.08 (s, 6H).
    • Step 3
  • Figure US20200115354A1-20200416-C00374
    • tert-butyl((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)carbamate
  • To a solution of tert-butyl((1S,6S)-6-((2-((tert-butyldimethylsilyl)oxy)ethyl)(methyl)amino)-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate (150 mg, 0.33 mmol) in EtOAc (10 mL) was added Pd(OH)2 (20%, 233 mg, 0.33 mmol). The mixture was stirred at room temperature for 16 h under hydrogen atmosphere (15 psi). The mixture was filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (solvent gradient: 0-10% EtOAc in petroleum ether) to give the title compound (60 mg, 40%) as colorless oil. 1H NMR (400 MHz, CDCl3) δ 5.39 (s, 1H), 3.74-3.60 (m, 2H), 3.24-3.12 (m, 1H), 2.70-2.57 (m, 2H), 2.56-2.47 (m, 1H), 2.44-2.35 (m, 1H), 2.24 (s, 3H), 2.11-1.97 (m, 2H), 1.94-1.84 (m, 1H), 1.45 (s, 9H), 1.45-1.40 (m, 1H), 1.38-1.25 (m, 2H), 1.16-1.03 (m, 1H), 0.91 (s, 9H), 0.08 (s, 6H).
    • Step 4
  • Figure US20200115354A1-20200416-C00375
    • 2-(((1S,2S,5S)-2-amino-5-(trifluoromethyl)cyclohexyl)(methyl)amino)ethanol
  • To a solution of tert-butyl((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)carbamate (60 mg, 0.13 mmol) in DCM (0.5 mL) was added trifluoroacetic acid (0.2 mL, 2.64 mmol). The mixture was stirred at room temperature for 2 h and concentrated in vacuo to give the title compound (31 mg, crude) as light yellow oil that required no further purification.
    • Step 5
  • Figure US20200115354A1-20200416-C00376
    • 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S,4S)-2-((2-hydroxyethyl)(methyl)amino)-4-(trifluoromethyl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide (0.18 g, 0.39 mmol) and N,N-diisopropylethylamine (0.21 mL, 1.29 mmol) in DMF (5 mL) was added 2-(((1S,2S,5S)-2-amino-5-(trifluoromethyl)cyclohexyl)(methyl)amino)ethanol (31 mg, 0.13 mmol). The mixture was heated to 70° C. for 16 h. After cooling to room temperature, the reaction was concentrated in vacuo. The crude residue was purified by silica gel chromatography (solvent gradient: 0-10%-25% EtOAc (1% TEA) in petroleum ether) to give the title compound (100 mg, 68%) as a light yellow solid. LCMS (ESI) m/z: 681.3 [M+H]+. H NMR (400 MHz, CDCl3) δ 7.74 (d, J=7.2 Hz, 1H), 7.40 (d, J=3.6 Hz, 1H), 7.22 (d, J=8.0 Hz, 1H), 6.98 (d, J=3.6 Hz, 1H), 6.42-6.33 (m, 2H), 6.29 (d, J=12.4 Hz, 1H), 5.58 (s, 1H), 5.20 (s, 2H), 3.76 (s, 3H), 3.75 (s, 3H), 3.63 (t, J=5.2 Hz, 2H), 3.20-3.10 (m, 1H), 2.72-2.54 (m, 3H), 2.45-2.35 (m, 1H), 2.23 (s, 3H), 2.18-2.10 (m, 1H), 2.06-1.98 (m, 1H), 1.53-1.39 (m, 2H), 1.31-1.18 (m, 2H).
    • Step 6
  • Figure US20200115354A1-20200416-C00377
    • 5-chloro-2-fluoro-4-(((1S,2S,4S)-2-((2-hydroxyethyl)(methyl)amino)-4-(trifluoromethyl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • A mixture of 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S,4S)-2-((2-hydroxyethyl)(methyl)amino)-4-(trifluoromethyl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (100 mg, 0.09 mmol) and formic acid (3.0 mL) was stirred at room temperature for 1 h. The reaction mixture was concentrated in vacuo. The crude residue was purified by reverse phase chromatography (acetonitrile 22-52%/0.225% formic acid in water) to give the title compound (19 mg, 41%) as a white solid. LCMS (ESI) m/z: 531.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.58 (d, J=7.2 Hz, 1H), 7.26 (d, J=4.4 Hz, 1H), 6.86-6.72 (m, 2H), 5.93 (d, J=4.4 Hz, 1H), 3.46-3.38 (m, 3H), 2.83 (t, J=9.6 Hz, 1H), 2.61-2.53 (m, 2H), 2.45-2.36 (m, 1H), 2.17 (s, 3H), 2.20-2.10 (m, 1H), 2.02-1.94 (m, 1H), 1.48-1.78 (m, 1H), 1.59-1.44 (m, 1H), 1.40-1.15 (m, 2H).
    • Example 139
  • Figure US20200115354A1-20200416-C00378
    • 5-Chloro-4-(((1S,2S)-2-(((S)-2-cyclopropyl-2-hydroxyethyl)(methyl)amino) cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00379
    Figure US20200115354A1-20200416-C00380
    • Step 1
  • Figure US20200115354A1-20200416-C00381
    • 5-Chloro-4-(((1S,2S)-2-((2-cyclopropyl-2-oxoethyl)(methyl)amino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • To 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (250 mg, 0.44 mmol) in DMF (2 mL) was added triethylamine (0.09 mL, 0.66 mmol) followed by a solution of 2-bromo-1-cyclopropyl-ethanone (72 mg, 0.44 mmol) in DMF (1 mL). The mixture was then stirred at room temperature for 3 days. The reaction was diluted with EtOAc (50 mL), washed with saturated aqueous NaHCO3 solution (10 mL), then with 50% aqueous NaCl solution (4×10 mL), then dried over Na2SO4, filtered and concentrated in vacuo. The crude product was purified by flash chromatography through Si gel (0-100% EtOAc/Hexanes) to provide 5-chloro-4-(((1S,2S)-2-((2-cyclopropyl-2-oxoethyl)(methyl)amino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (144 mg, 50% yield) as a yellow wax. LCMS (ESI) m/z: 651.0 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00382
    • 5-Chloro-4-(((1S,2S)-2-(((S)-2-cyclopropyl-2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1S,2S)-2-(((R)-2-cyclopropyl-2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • 5-Chloro-4-(((1S,2S)-2-((2-cyclopropyl-2-oxoethyl)(methyl)amino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (144 mg, 0.22 mmol) was dissolved in a mixture of iPrOH (3 mL) and THF (0.5 mL). This solution was cooled to 0° C. and to this was added sodium borohydride (25 mg, 0.66 mmol). After 90 min, the mixture was removed from the cooling bath and a further portion of sodium borohydride (25 mg, 0.66 mmol) was added and stirred at room temperature. After 1 h, a further portion of sodium borohydride (75 mg, 1.98 mmol) was added and stirred for 16 h. MeOH (5 mL) was added and concentrated in vacuo (repeated 3 times). The crude residue was diluted with EtOAc (50 mL) and saturated aqueous solution of NaHCO3 (15 mL) and the phases were separated. The organic extract was dried (Na2SO4), filtered and concentrated in vacuo to provide 5-chloro-4-(((1S,2S)-2-((2-cyclopropyl-2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (111 mg, 77%) as a white waxy semi-solid as a mixture of alcohol diastereomers. The mixture of 5-chloro-4-(((1S,2S)-2-((2-cyclopropyl-2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide alcohol diastereomers prepared above (111 mg, 0.17 mmol) was separated using chiral HPLC (ChiralPak IA, 250 mm×20 mm ID, 5 μm), 5:5:90 MeOH:DCM:Hexanes, 22 min) to afford 5-chloro-4-(((1S,2S)-2-(((S)-2-cyclopropyl-2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (first peak, 65 mg, 59%) and 5-chloro-4-(((1S,2S)-2-(((R)-2-cyclopropyl-2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (second peak, 39 mg, 35%). Absolute alcohol configuration was arbitrarily assigned to each isomer. LCMS (ESI) m/z: 653.1 [M+H]+.
    • Step 3
  • Figure US20200115354A1-20200416-C00383
    • 5-Chloro-4-(((1S,2S)-2-(((S)-2-cyclopropyl-2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 115 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-((2-hydroxy-2-methylpropyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-4-(((1S,2S)-2-(((S)-2-cyclopropyl-2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (65 mg, 0.10 mmol), 5-chloro-4-(((1S,2S)-2-(((S)-2-cyclopropyl-2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (21 mg, 42%) was obtained. LCMS (ESI) m/z: 503.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.73-11.82 (m, 1H), 7.56 (d, J=7.3 Hz, 1H), 7.23 (d, J=4.5 Hz, 1H), 6.78 (d, J=4.5 Hz, 1H), 6.72 (d, J=13.0 Hz, 1H), 5.96 (d, J=4.0 Hz, 1H), 4.69-3.67 (m, 1H), 3.04 (dd, J=11.4, 5.9 Hz, 1H), 2.75-2.65 (m, 1H), 2.44-2.36 (m, 1H), 2.19 (s, 3H), 2.14 (d, J=23.8 Hz, 1H), 2.08 (s, 2H), 1.79 (dd, J=28.7, 10.6 Hz, 2H), 1.59 (d, J=12.3 Hz, 1H), 1.46-1.04 (m, 4H), 0.77-0.66 (m, 1H), 0.28-0.17 (m, 1H), 0.17-0.02 (m, 3H).
    • Example 140
  • Figure US20200115354A1-20200416-C00384
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(methyl(1-methyl-1H-pyrazol-4-yl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00385
    • Step 1
  • Figure US20200115354A1-20200416-C00386
    • 5-Chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-((1,3-dioxopropan-2-yl)(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(methylamino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (180 mg, 0.32 mmol) and 2-chloromalonaldehyde (34 mg, 0.32 mmol) in MeCN (3 mL) and DMF (1 mL) was added triethylamine (48 mg, 0.066 mL, 0.47 mmol) and the mixture stirred at rt overnight. After 16 h, a further portion of triethylamine (48 mg, 0.066 mL, 0.47 mmol) was added and the mixture placed in a 65° C. oil bath sealed. After 3 days, the crude mixture of 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-((1,3-dioxopropan-2-yl)(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide was used directly in the next step as is. LCMS (ESI) m/z: 656.9 [M+NH4]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00387
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(methyl(1-methyl-1H-pyrazol-4-yl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-((1,3-dioxopropan-2-yl)(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (1.33 mL of the crude solution prepared above in step 1, 65 mg, 0.10 mmol) was added to a solution of methylhydrazine sulfate (44 mg, 0.31 mmol) in MeOH (0.5 mL). To this mixture was then added 4N HCl in dioxanes (0.05 mL, 0.20 mmol) and the flask placed in a 65° C. oil bath sealed. After 2 h, volatiles were removed under an air stream and the crude residue purified by C18 reverse phase flash chromatography (0-100% MeCN/10 mM aqueous NH4CO2H, pH=3.8). Appropriate fractions were combined and lyophilized to provide 5-chloro-2-fluoro-4-(((1S,2S)-2-(methyl(1-methyl-1H-pyrazol-4-yl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (8 mg, 16%) as a tan powder. LCMS (ESI) m/z: 499.0 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 12.77 (s, 1H), 7.55 (d, J=7.3 Hz, 1H), 7.20 (d, J=1.7 Hz, 1H), 7.14 (d, J=3.4 Hz, 1H), 6.77 (d, J=13.1 Hz, 1H), 6.68 (d, J=2.9 Hz, 1H), 5.78 (d, J=1.7 Hz, 1H), 5.66 (d, J=7.4 Hz, 1H), 3.70-3.56 (m, 1H), 3.54 (s, 3H), 3.01 (td, J=11.8, 3.3 Hz, 1H), 2.48 (s, 3H), 1.97-1.82 (m, 2H), 1.76-1.68 (m, 1H), 1.63-1.53 (m, 2H), 1.40-1.31 (m, 2H), 1.26-1.18 (m, 1H).
    • Example 141
  • Figure US20200115354A1-20200416-C00388
    • 5-Chloro-4-(((1S,6S)-6-(dimethylamino)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Figure US20200115354A1-20200416-C00389
    Figure US20200115354A1-20200416-C00390
    • Step 1
  • Figure US20200115354A1-20200416-C00391
    • Rac-4-(((1,6-trans)-6-aminocyclohex-3-en-1-yl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide (2.29 g, 4.96 mmol) in DMF (10 mL) was added triethylamine (2.51 g, 3.46 mL, 24.8 mmol) followed by addition of rac-trans-cyclohex-4-ene-1,2-diamine dihydrochloride (1.01 g, 5.46 mmol) and the mixture stirred at rt overnight. After 16 h, the mixture was diluted with EtOAc (100 mL), washed with saturated NaHCO3(aq) (25 mL), then with 50% saturated NaCl(aq) (4×10 mL), dried (Na2SO4), filtered and concentrated in vacuo. Purification by flash column chromatography through Si gel (0-100% EtOAc/hexanes) provided rac-4-(((1,6-trans)-6-aminocyclohex-3-en-1-yl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (1.33 g, 48%) as a waxy semi-solid. LCMS (ESI) m/z: 553.1 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00392
    • Rac-5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1,6-trans)-6-(dimethylamino)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of rac-4-(((1,6-trans)-6-aminocyclohex-3-en-1-yl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (1.33 g, 2.40 mmol) in MeOH (20 mL) was added 37% w/w aqueous formaldehyde (1.95 g, 1.79 mL, 24.0 mmol) followed by addition of sodium cyanoborohydride (446 mg, 7.21 mmol) and the mixture was stirred at rt. After 3 days, a further portion of 37% w/w aqueous formaldehyde (975 mg, 0.90 mL, 12.0 mmol) was added followed by addition of a further portion of sodium cyanoborohydride (446 mg, 7.21 mmol) and continued stirring at rt. After 3 h, volatiles were removed in vacuo and the crude residue partitioned between EtOAc (50 mL) and saturated NaHCO3(aq) (10 mL). The phases were separated and the organic extract dried (Na2SO4), filtered and concentrated in vacuo. Purification by flash column chromatography through Si gel (0-100% EtOAc/CH2Cl2) provided rac-5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1,6-trans)-6-(dimethylamino)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (971 mg, 69%) as a clear waxy gum. LCMS (ESI) m/z: 581.1 [M+H]+.
    • Step 3
  • Figure US20200115354A1-20200416-C00393
    • 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,6S)-6-(dimethylamino)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1R,6R)-6-(dimethylamino)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Rac-5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1,6-trans)-6-(dimethylamino)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (837 mg, 1.44 mmol) was separated by using chiral HPLC (Chiralpak IB (250 mm*20 mm, 5 um), MeOH:DCM:hexane 1:4:95 containing 0.1% of diethylamine, 15 ml/min) to afford 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,6S)-6-(dimethylamino)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (first peak, 367 mg, 43%) as a white solid. LCMS (ESI) m/z: 581.1 [M+H]+. Also afforded afford 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1R,6R)-6-(dimethylamino)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (second peak, 366 mg, 43%) as a white solid. LCMS (ESI) m/z: 581.1 [M+H]+.
    • Step 4
  • Figure US20200115354A1-20200416-C00394
    • 5-Chloro-4-(((1S,6S)-6-(dimethylamino)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 115 and making non-critical variations to substitute 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-((2-hydroxy-2-methylpropyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,6S)-6-(dimethylamino)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide, 5-chloro-4-(((1S,6S)-6-(dimethylamino)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate (41 mg, 99%) was obtained as a white solid. LCMS (ESI) m/z: 430.9 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 8.16 (s, 1H), 7.59 (d, J=7.3 Hz, 1H), 7.18 (d, J=4.3 Hz, 1H), 6.78-6.70 (m, 2H), 6.09 (d, J=3.9 Hz, 1H), 5.72-5.64 (m, 1H), 5.62-5.52 (m, 1H), 3.52 (ddd, J=15.1, 10.0, 5.0 Hz, 1H), 3.00 (td, J=10.5, 5.4 Hz, 1H), 2.70 (dt, J=16.4, 4.5 Hz, 1H), 2.21 (s, 6H), 2.19-2.05 (m, 2H), 1.97-1.85 (m, 1H). Sulfonamide N—H and formic acid O—H not visible in the NMR.
    • Example 142
  • Figure US20200115354A1-20200416-C00395
    • 5-Chloro-4-(((1R,6R)-6-(dimethylamino)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 115 and making non-critical variations to substitute 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-((2-hydroxy-2-methylpropyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((R,6R)-6-(dimethylamino)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide, 5-chloro-4-(((1R,6R)-6-(dimethylamino)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate (41 mg, 99%) was obtained as a white solid. LCMS (ESI) m/z: 431.90 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 8.15 (s, 1H), 7.59 (d, J=7.3 Hz, 1H), 7.20 (d, J=4.4 Hz, 1H), 6.80-6.69 (m, 2H), 6.10 (d, J=3.9 Hz, 1H), 5.75-5.64 (m, 1H), 5.63-5.51 (m, 1H), 3.56-3.49 (m, 1H), 3.01 (td, J=10.5, 5.4 Hz, 1H), 2.70 (dt, J=9.7, 4.2 Hz, 1H), 2.22 (s, 6H), 2.20-2.05 (m, 2H), 1.97-1.87 (m, 1H). Sulfonamide N—H and formic acid O—H not visible.
    • Example 143
  • Figure US20200115354A1-20200416-C00396
    • 5-Chloro-4-(((1S,2S)-2-(((R)-2-cyclopropyl-2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 115 and making non-critical variations to substitute 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-((2-hydroxy-2-methylpropyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-4-(((1S,2S)-2-(((R)-2-cyclopropyl-2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (39 mg, 0.06 mmol), 5-chloro-4-(((1S,2S)-2-(((R)-2-cyclopropyl-2-hydroxyethyl)(methyl)amino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (16 mg, 52%) was obtained as a white solid. LCMS (ESI) m/z: 502.9 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.38 (bs, 1H), 7.56 (d, J=7.3 Hz, 1H), 7.22 (d, J=4.5 Hz, 1H), 6.77 (d, J=4.5 Hz, 1H), 6.71 (d, J=13.1 Hz, 1H), 5.95 (d, J=4.7 Hz, 1H), 3.06-2.95 (m, 1H), 2.77-2.62 (m, 1H), 2.22 (s, 3H), 2.14-2.04 (m, 1H), 1.93-1.67 (m, 2H), 1.59 (d, J=12.4 Hz, 1H), 1.49-0.99 (m, 4H), 0.89-0.68 (m, 1H), 0.27 (dd, J=8.3, 2.6 Hz, 2H), 0.26-0.09 (m, 2H).
    • Example 144
  • Figure US20200115354A1-20200416-C00397
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)cycloheptyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 141 and making non-critical variations to substitute rac-trans-cyclohex-4-ene-1,2-diamine with rac-trans-cycloheptane-1,2-diamine the title compound was obtained. The absolute stereochemistry was assigned arbitrarily. LCMS (ESI) m/z: 447.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.22 (s, 1H), 7.57 (d, J=7.2 Hz, 1H), 7.05 (s, 1H), 6.58 (s, 1H), 6.45 (d, J=12.6 Hz, 1H), 5.93 (s, 1H), 3.36-3.29 (m, 1H), 2.74 (t, J=8.1 Hz, 1H), 2.16 (s, 6H), 1.81-1.69 (m, 3H), 1.59-1.31 (m, 7H) [acidic N—H and O—H not observed].
    • Example 145
  • Figure US20200115354A1-20200416-C00398
    • 5-chloro-4-(((1R,2R)-2-(dimethylamino)cycloheptyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 141 and making non-critical variations to substitute rac-trans-cyclohex-4-ene-1,2-diamine with rac-trans-cycloheptane-1,2-diamine the title compound was obtained. The absolute stereochemistry was assigned arbitrarily. LCMS (ESI) m/z: 447.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.18 (s, 1H), 7.57 (d, J=7.3 Hz, 1H), 7.08 (d, J=4.1 Hz, 1H), 6.61 (d, J=4.1 Hz, 1H), 6.48 (d, J=12.7 Hz, 1H), 5.96 (d, J=3.8 Hz, 1H), 3.35 (s, 1H), 2.78 (t, J=7.7 Hz, 1H), 2.17 (s, 6H), 1.84-1.65 (m, 3H), 1.64-1.28 (m, 7H) [acidic N—H and O—H not observed].
    • Example 146
  • Figure US20200115354A1-20200416-C00399
    • 5-Chloro-2-fluoro-4-(((1S,2S)-2-((1-isobutyrylazetidin-3-yl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of 4-(((1S,2S)-2-(azetidin-3-yl(methyl)amino)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide hydrochloride (80 mg, 0.16 mmol) in a mixture of CH2Cl2 (1 mL) and pyridine (0.38 mL, 4.7 mmol) was added isobutyryl chloride (20 uL, 0.19 mmol). The reaction mixture was stirred at room temperature for 16 hours then concentrated to dryness. The crude was directly purified by C18 reverse phase flash chromatography (5-70% MeCN/10 mM aqueous ammonium bicarbonate, pH=10). Appropriate fractions combined and lyophilized to provide 5-chloro-2-fluoro-4-(((1S,2S)-2-((1-isobutyrylazetidin-3-yl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (26 mg, 30%). LCMS (ESI) m/z: 544.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.68 (br.s, 1H), 7.56 (dd, J=7.3, 2.2 Hz, 1H), 7.26 (d, J=4.7 Hz, 1H), 6.79 (dd, J=23.4, 8.8 Hz, 2H), 5.80 (s, 1H), 4.16-4.02 (m, 1H), 4.00-3.89 (m, 1H), 3.85-3.58 (m, 3H), 2.64-2.53 (m, 1H), 2.45-2.35 (m, 1H), 2.04 (s, 4H), 1.82-1.52 (m, 3H), 1.42-1.09 (m, 5H), 1.00-0.85 (m, 7H).
    • Example 147
  • Figure US20200115354A1-20200416-C00400
    • 5-Chloro-2-fluoro-4-(((1S,2S)-2-(methyl(1-(3-methylbutanoyl)azetidin-3-yl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 146 and making non-critical variations as required to replace isobutyryl chloride with isovaleryl chloride, 5-chloro-2-fluoro-4-(((1S,2S)-2-(methyl(1-(3-methylbutanoyl)azetidin-3-yl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 558.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.75 (bs, 1H), 7.56 (dd, J=7.3, 2.1 Hz, 1H), 7.23 (d, J=4.5 Hz, 1H), 6.76 (dd, J=16.5, 8.8 Hz, 2H), 5.76 (d, J=5.9 Hz, 1H), 4.04 (dd, J=18.3, 8.1 Hz, 1H), 3.98-3.88 (m, 1H), 3.86-3.73 (m, 2H), 3.72-3.56 (m, 2H), 2.12-1.97 (m, 5H), 1.99-1.79 (m, 4H), 1.79-1.51 (m, 3H), 1.25 (dt, J=25.8, 11.9 Hz, 4H), 0.94-0.77 (m, 7H).
    • Example 148
  • Figure US20200115354A1-20200416-C00401
    • 4-(((1S,2S)-2-((1-Acetylazetidin-3-yl)(methyl)amino)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 146 and making non-critical variations as required to replace isobutyryl chloride with acetyl bromide, 4-(((1S,2S)-2-((1-acetylazetidin-3-yl)(methyl)amino)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 515.8 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.74 (s, 1H), 7.57 (d, J=7.2 Hz, 1H), 7.20 (s, 1H), 6.82-6.59 (m, 2H), 5.75 (s, 1H), 4.09-3.97 (m, 1H), 3.97-3.89 (m, 1H), 3.85-3.71 (m, 2H), 3.71-3.59 (m, 2H), 3.57-3.48 (m, 1H), 3.44-3.37 (m, 1H), 2.64-2.56 (m, 1H), 2.04 (d, J=2.6 Hz, 3H), 2.02-1.99 (m, 1H), 1.71 (d, J=8.6 Hz, 3H), 1.66-1.56 (m, 1H), 1.42-1.15 (m, 4H).
    • Example 149
  • Figure US20200115354A1-20200416-C00402
    • 5-Chloro-4-(((1S,2S)-2-(4-cyanopiperidin-1-yl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 70 and making non-critical variations as required to replace piperidine with 4-cyanopiperidine, 5-Chloro-4-(((1S,2S)-2-(4-cyanopiperidin-1-yl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate (100 mg, 84%) was obtained. LCMS (ESI) m/z: 498.1 (M+H)+. 1H NMR (400 MHz, d6-DMSO) δ 8.19 (s, 1H), 7.58 (d, J=7.2 Hz, 1H), 7.13 (d, J=4.2 Hz, 1H), 6.74-6.55 (m, 2H), 5.85-5.75 (m, 1H), 3.27-3.12 (m, 1H), 2.87-2.72 (m, 1H), 2.65-2.52 (m, 2H), 2.48-2.39 (m, 2H), 2.36-2.24 (m, 1H), 2.16 (d, J=10.9 Hz, 1H), 1.84-1.69 (m, 3H), 1.68-1.53 (m, 3H), 1.53-1.31 (m, 2H), 1.29-1.07 (m, 3H).
    • Example 150
  • Figure US20200115354A1-20200416-C00403
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(4-hydroxy-4-methylpiperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 70 and making non-critical variations as required to replace piperidine with 4-methylpiperidin-4-ol, 5-chloro-2-fluoro-4-(((1S,2S)-2-(4-hydroxy-4-methylpiperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide formate was obtained. LCMS (ESI) m/z: 503.1 (M+H)+. 1H NMR (400 MHz, d6-DMSO) δ 8.12 (s, 1H), 7.55 (d, J=7.3 Hz, 1H), 7.16 (d, J=4.4 Hz, 1H), 6.71 (d, J=4.4 Hz, 1H), 6.62 (d, J=12.7 Hz, 1H), 6.06-5.95 (m, 1H), 3.23-3.08 (m, 1H), 2.75-2.60 (m, 1H), 2.48-2.44 (m, 4H), 2.39 (t, J=9.5 Hz, 1H), 2.32-2.19 (m, 2H), 2.15 (d, J=11.1 Hz, 1H), 1.88-1.78 (m, 1H), 1.76-1.66 (m, 1H), 1.58 (d, J=11.7 Hz, 1H), 1.43-1.27 (m, 3H), 1.27-1.03 (m, 3H), 0.98 (s, 3H).
    • Example 151 & Example 152
  • Figure US20200115354A1-20200416-C00404
    • 4-(((1S,2S)-2-(3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-5-chloro-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide & 4-(((1R,2R)-2-(3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-5-chloro-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 105 & Example 106 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide, 4-(((1S,2S)-2-(3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-5-chloro-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide was obtained as a white solid. Example 150: LCMS (ESI) m/z: 466.1 [M+H]+. H NMR (400 MHz, DMSO-d6) δ 8.89 (s, 1H), 8.61 (s, 1H), 8.32 (s, 1H), 7.82-7.67 (m, 1H), 7.13-6.86 (m, 2H), 6.38-6.17 (m, 1H), 3.91-3.69 (m, 3H), 3.39-3.18 (m, 3H), 3.06-2.94 (m, 1H), 2.26-2.12 (m, 1H), 1.86-1.71 (m, 5H), 1.66-1.54 (m, 1H), 1.51-1.11 (m, 5H), 0.72 (q, J=7.6 Hz, 1H), 0.53 (q, J=4.7 Hz, 1H). 4-(((1R,2R)-2-(3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-5-chloro-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide was obtained as a white solid. Example 151: LCMS (ESI) m/z: 466.1 [M+H]+. H NMR (400 MHz, DMSO-d6) δ 8.89 (s, 1H), 8.61 (s, 1H), 8.32 (s, 1H), 7.83-7.65 (m, 1H), 7.13-6.79 (m, 2H), 6.36-6.16 (m, 1H), 3.87-3.70 (m, 5H), 3.40-3.13 (m, 2H), 3.10-2.91 (m, 1H), 2.25-2.13 (m, 1H), 1.89-1.68 (m, 4H), 1.66-1.52 (m, 1H), 1.49-1.25 (m, 2H), 1.24 (s, 3H), 0.72 (td, J=8.0, 5.6 Hz, 1H), 0.53 (q, J=4.7 Hz, 1H).
    • Example 153
  • Figure US20200115354A1-20200416-C00405
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-((S)-3-hydroxypiperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 70 and making non-critical variations as required to replace the piperidine with (S)-piperidin-3-ol, the title compound was obtained. LCMS (ESI) m/z: 489.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.69 (s, 1H), 7.60 (d, J=7.3 Hz, 1H), 7.25 (d, J=4.5 Hz, 1H), 6.81 (d, J=4.6 Hz, 1H), 6.71 (d, J=12.8 Hz, 1H), 6.03 (dd, J=3.7, 1.6 Hz, 1H), 4.51 (d, J=4.6 Hz, 1H), 3.43-3.15 (m, 5H), 2.64-2.50 (m, 2H), 2.50-2.33 (m, 1H), 2.20 (d, J=12.1 Hz, 1H), 1.83 (t, J=8.2 Hz, 1H), 1.75 (s, 1H), 1.60 (t, J=8.2 Hz, 2H), 1.40 (d, J=11.2 Hz, 2H), 1.25-0.93 (m, 4H).
    • Example 154
  • Figure US20200115354A1-20200416-C00406
    • 4-(((1S,2S)-2-(6-Azaspiro[2.5]octan-6-yl)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the synthetic sequence described in Example 70 and making non-critical variations to substitute piperidine with 6-azaspiro[2.5]octane, 4-(((1S,2S)-2-(6-Azaspiro[2.5]octan-6-yl)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate was obtained. LCMS (ESI) m/z: 499.0 (M+H)+. 1H NMR (400 MHz, cdcl3) δ 7.85 (d, J=7.3 Hz, 1H), 7.15 (d, J=4.6 Hz, 1H), 6.50 (d, J=4.6 Hz, 1H), 6.35 (s, 1H), 6.31 (d, J=12.6 Hz, 1H), 3.07-2.98 (m, 1H), 2.82-2.70 (m, 1H), 2.64-2.56 (m, 2H), 2.54-2.42 (m, 2H), 2.30 (d, J=13.1 Hz, 1H), 2.06-1.96 (m, 1H), 1.93-1.83 (m, 1H), 1.79-1.70 (m, 1H), 1.50-1.10 (m, 8H), 0.25 (s, 4H).
    • Example 155
  • Figure US20200115354A1-20200416-C00407
    • 5-Chloro-4-(((1S,2S)-2-((1-(cyclopropanecarbonyl)azetidin-3-yl)(methyl)amino)-cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 146 and making non-critical variations as required to replace isobutyryl chloride with cyclopropanecarbonyl chloride, 5-chloro-4-(((1S,2S)-2-((1-(cyclopropanecarbonyl)azetidin-3-yl)(methyl)amino)-cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide was obtained as an off-white solid. LCMS (ESI) m/z: 542.1 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 8.15 (s, 1H), 7.56 (d, J=7.0 Hz, 1H), 7.15 (d, J=3.9 Hz, 1H), 6.75-6.64 (m, J=13.1 Hz, 2H), 5.76-5.61 (m, 1H), 4.18 (dt, J=15.1, 7.6 Hz, 1H), 4.09-3.86 (m, 1H), 3.83-3.74 (m, 1H), 3.74-3.64 (m, J=5.4 Hz, 2H), 3.60-3.52 (m, 1H), 2.59 (t, J=9.5 Hz, 1H), 2.05 (s, 4H), 1.76-1.64 (m, 2H), 1.60 (d, J=11.5 Hz, 1H), 1.55-1.40 (m, 1H), 1.40-1.04 (m, 4H), 0.76-0.55 (m, 4H).
    • Example 156
  • Figure US20200115354A1-20200416-C00408
    • 5-Chloro-2-fluoro-4-(((1S,2S)-2-((cis-3-hydroxycyclobutyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 18 and making non-critical variations as required to replace acetaldehyde with 3-hydroxycyclobutane, the title compound was obtains as a mixture of diastereomers. Separation of isomers by HPLC gave 5-Chloro-2-fluoro-4-(((1S,2S)-2-((cis-3-hydroxycyclobutyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide formate as a white solid. LCMS (ESI) m/z: 489.1 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 12.61 (s, 1H), 8.14 (s, 1H), 7.59 (d, J=7.3 Hz, 1H), 7.23 (d, J=4.5 Hz, 1H), 6.78 (d, J=4.5 Hz, 1H), 6.72 (d, J=12.9 Hz, 1H), 5.82 (d, J=3.5 Hz, 1H), 4.92 (s, 1H), 4.13 (tt, J=7.2, 3.8 Hz, 1H), 3.29-3.16 (m, 2H), 2.65-2.55 (m, 1H), 2.20-2.12 (m, 1H), 2.10-2.05 (m, 1H), 2.02-1.92 (m, 4H), 1.91-1.80 (m, 2H), 1.78-1.67 (m, 2H), 1.64-1.56 (m, 1H), 1.42-1.29 (m, 1H), 1.29-1.09 (m, 3H).
    • Example 157
  • Figure US20200115354A1-20200416-C00409
    • 5-Chloro-2-fluoro-4-(((1S,2S)-2-((trans-3-hydroxycyclobutyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 18 and making non-critical variations as required to replace acetaldehyde with 3-hydroxycyclobutane, the title compound was obtains as a mixture of diastereomers. Separation of isomers by HPLC gave 5-Chloro-2-fluoro-4-(((1S,2S)-2-((trans-3-hydroxycyclobutyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide formate as a white solid. LCMS (ESI) m/z: 489.1 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 12.78 (s, 1H), 8.14 (s, 1H), 7.60 (d, J=7.3 Hz, 1H), 7.21 (d, J=4.2 Hz, 1H), 6.76 (d, J=4.0 Hz, 1H), 6.69 (d, J=12.8 Hz, 1H), 5.84 (s, 1H), 4.99 (d, J=5.5 Hz, 1H), 3.72 (dd, J=14.1, 7.4 Hz, 1H), 3.24-3.12 (m, 1H), 2.64-2.56 (m, 1H), 2.38-2.26 (m, 3H), 2.17 (d, J=11.3 Hz, 1H), 1.94 (s, 3H), 1.78-1.65 (m, 2H), 1.65-1.45 (m, 3H), 1.42-1.29 (m, 1H), 1.27-1.04 (m, 3H).
    • Example 158
  • Figure US20200115354A1-20200416-C00410
    • 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S,4S)-2-((2-hydroxyethyl)(methyl)amino)-4-(trifluoromethyl)cyclohexyl)amino)benzenesulfonamide Formate
  • Following the procedure described in Example 138 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-2,4-difluoro-N-(6-fluoropyridin-2-yl)-N-(methoxymethyl)benzenesulfonamide, 5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-4-(((1S,2S,4S)-2-((2-hydroxyethyl)(methyl)amino)-4-(trifluoromethyl)cyclohexyl)amino)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 543.3 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.13 (s, 1H), 7.90-7.80 (m, 1H), 7.73 (d, J=7.6 Hz, 1H), 6.92-6.81 (m, 2H), 6.71 (dd, J=8.0, 2.4 Hz, 1H), 6.16 (d, J=5.2 Hz, 1H), 3.57-3.40 (m, 3H), 3.05-2.86 (m, 1H), 2.65-2.55 (m, 2H), 2.48-2.35 (m, 1H), 2.22 (s, 3H), 2.16-2.07 (m, 1H), 2.06-1.95 (m, 1H), 1.84-1.72 (m, 1H), 1.58-1.43 (m, 1H), 1.42-1.19 (m, 2H).
    • Example 159 & Example 160
  • Figure US20200115354A1-20200416-C00411
    • 4-(((1R,2R)-2-(3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 4-(((1S,2S)-2-(3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 105 & Example 106 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide, 4-(((1S,2S)-2-(3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide was obtained as a white solid. Example 158: LCMS (ESI) m/z: 471.1 [M+H]+. H NMR (400 MHz, DMSO-d6) δ 12.73 (s, 1H), 7.59 (d, J=7.3 Hz, 1H), 7.24 (d, J=4.5 Hz, 1H), 6.80 (d, J=4.5 Hz, 1H), 6.67 (d, J=12.9 Hz, 1H), 5.68 (dd, J=4.7, 1.8 Hz, 1H), 3.20-3.04 (m, 1H), 2.86-2.59 (m, 3H), 2.40-2.27 (m, 1H), 2.16-2.06 (m, 2H), 1.84-1.68 (m, 2H), 1.67-1.54 (m, 1H), 1.48-1.02 (m, 7H), 0.49-0.38 (m, 1H), 0.28-0.13 (m, 1H). 4-(((1R,2R)-2-(3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-5-chloro-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide was obtained as a white solid. Example 159: LCMS (ESI) m/z: 471.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.72 (s, 1H), 7.59 (d, J=7.3 Hz, 1H), 7.24 (d, J=4.6 Hz, 1H), 6.80 (d, J=4.5 Hz, 1H), 6.67 (d, J=12.9 Hz, 1H), 5.69 (dd, J=4.8, 1.9 Hz, 1H), 3.20-3.09 (m, 1H), 2.83-2.63 (m, 3H), 2.36 (d, J=8.2 Hz, 1H), 2.16-2.08 (m, 1H), 1.84-1.67 (m, 2H), 1.66-1.55 (m, 1H), 1.45-1.03 (m, 7H), 0.51-0.37 (m, 1H), 0.27-0.14 (m, 1H).
    • Example 161
  • Figure US20200115354A1-20200416-C00412
    • 5-Chloro-4-(((1S,2S)-2-((R)-3-cyanopyrrolidin-1-yl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the synthetic sequence described in Example 70 and making non-critical variations to substitute piperidine with (R)-pyrrolidine-3-carbonitrile, 5-chloro-4-(((1S,2S)-2-((R)-3-cyanopyrrolidin-1-yl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate was obtained. LCMS (ESI) m/z: 483.9 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.78 (bs, 1H), 8.15 (s, 1H), 7.56 (dd, J=7.1, 5.0 Hz, 1H), 7.14 (s, 1H), 6.67 (d, J=12.4 Hz, 2H), 5.71 (d, J=15.3 Hz, 1H), 3.17-3.02 (m, 1H), 2.93-2.76 (m, 2H), 2.78-2.58 (m, 2H), 2.60-2.51 (m, 1H), 2.20-1.93 (m, 2H), 1.92-1.66 (m, 3H), 1.60 (d, J=13.9 Hz, 1H), 1.48-1.06 (m, 4H).
    • Example 162
  • Figure US20200115354A1-20200416-C00413
    • 5-Chloro-4-(((1S,2S)-2-((S)-3-cyanopyrrolidin-1-yl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the synthetic sequence described in Example 70 and making non-critical variations to substitute piperidine with (S)-pyrrolidine-3-carbonitrile, 5-chloro-4-(((1S,2S)-2-((S)-3-cyanopyrrolidin-1-yl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate was obtained. LCMS (ESI) m/z: 484.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.74 (br.s, 1H), 7.57 (dd, J=7.3, 5.4 Hz, 1H), 7.23 (d, J=4.5 Hz, 1H), 6.78 (d, J=4.5 Hz, 1H), 6.72 (d, J=12.9 Hz, 1H), 5.80 (dd, J=16.3, 4.8 Hz, 1H), 3.18-3.03 (m, 1H), 2.92-2.77 (m, 3H), 2.77-2.58 (m, 2H), 2.59-2.52 (m, 1H), 1.91-1.68 (m, 3H), 1.60 (d, J=13.2 Hz, 1H), 1.48-1.09 (m, 4H).
    • Example 163
  • Figure US20200115354A1-20200416-C00414
    • 5-Chloro-2-fluoro-4-(((1S,2S)-2-(4-methylpiperazin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the synthetic sequence described in Example 70 and making non-critical variations to substitute piperidine with 1-methylpiperazine, 5-Chloro-2-fluoro-4-(((1S,2S)-2-(4-methylpiperazin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide was obtained. LCMS (ESI) m/z: 488.0 (M+H)+. 1H NMR (400 MHz, d6-dmso) δ 7.59 (d, J=7.3 Hz, 1H), 7.20 (d, J=4.4 Hz, 1H), 6.75 (d, J=4.4 Hz, 1H), 6.68 (d, J=12.9 Hz, 1H), 5.91 (d, J=3.3 Hz, 1H), 3.27-3.17 (m, 1H), 2.63-2.22 (m, 9H), 2.16 (s, 3H), 2.16-2.09 (m, 1H), 1.86-1.70 (m, 2H), 1.66-1.55 (m, 1H), 1.45-1.07 (m, 4H).
    • Example 164
  • Figure US20200115354A1-20200416-C00415
    • 5-Chloro-4-(((2R,3R)-3-(dimethylamino)-1,2,3,4-tetrahydronaphthalen-2-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00416
    • Step 1
  • Figure US20200115354A1-20200416-C00417
    • Rac-trans-1,2,3,4-tetrahydronaphthalene-2,3-diol
  • To a mixture of formic acid (8.84 g, 192 mmol) and hydrogen peroxide (30% w/w in H2O) (1.04 g, 9.22 mmol) was added 1,4-dihydronaphthalene (1.0 g, 7.68 mmol). The immiscible layers are stirred vigorously and the suspension becomes homogeneous after 10 min. The reaction was heated at 60° C. and it is held for 1 hour. Most of the formic acid is removed by distillation and the residue is heated for 45 min at 60° C. with 5 mL NaOH (20% in water). After cooling, the yellow solution is neutralized with HCl (6 M) until pH=1 and extracted with EtOAc (20 mL). The organic extract was dried over MgSO4, filtered and concentrated in vacuo to give crude trans-1,2,3,4-tetrahydronaphthalene-2,3-diol (1.26 g), which was used directly in the next step without further purification. 1H NMR (400 MHz, CDCl3) δ 7.16-7.07 (m, 4H), 3.95-3.86 (m, 2H), 3.24-3.16 (m, 2H), 2.89-2.79 (m, 2H).
  • Figure US20200115354A1-20200416-C00418
    • Rac-trans-1,2,3,4-tetrahydronaphthalene-2,3-diyl dimethanesulfonate
  • Methanesulfonyl chloride (1.19 mL, 15 mmol) was added at 0° C. to a solution of rac-trans-1,2,3,4-tetrahydronaphthalene-2,3-diol (0.63 g, 3.84 mmol) in pyridine (4 mL). The reaction was warmed to rt and stirred for 1 hour until completion. The reaction mixture was diluted with cold water (10 mL) and extracted with EtOAc (3×10 mL). The combined organic layers were washed with HCl (0.5N), followed by brine (10 mL). The organic phase was dried over Na2SO4 and the solvent was removed in vacuo to give rac-trans-1,2,3,4-tetrahydronaphthalene-2,3-diyl dimethanesulfonate (1.23 g, 3.8 mmol, 99% yield). 1H NMR (400 MHz, CDCl3) δ 7.23-7.16 (m, 2H), 7.15-7.04 (m, 2H), 5.07 (ddt, J=9.5, 8.3, 4.2 Hz, 2H), 3.53-3.43 (m, 2H), 3.18 (ddd, J=10.4, 5.5, 3.0 Hz, 2H).
    • Step 3
  • Figure US20200115354A1-20200416-C00419
    • Rac-trans-2,3-diazido-1,2,3,4-tetrahydronaphthalene
  • Sodium azide (4.0 g, 62.4 mmol) was added at 120° C. to a solution of rac-trans-1,2,3,4-tetrahydronaphthalene-2,3-diyl dimethanesulfonate (2.0 g, 6.24 mmol) in DMF (3 mL, anhydrous). The reaction was stirred for 2 hour until completion. The reaction mixture was diluted with cold water (30 mL) and extracted with EtOAc (3×30 mL). The combined organic layers were washed with HCl (0.5 N) then with brine (10 mL). The organic phase was dried over Na2SO4 and the solvent was removed in vacuo to give rac-trans-2,3-diazido-1,2,3,4-tetrahydronaphthalene (1.2 g, 90% yield). The crude mixture was used without purification to the next step. 1H NMR (400 MHz, CDCl3) δ 7.20-7.15 (m, 2H), 7.13-7.08 (m, 2H), 3.80-3.69 (m, 2H), 3.26-3.19 (m, 2H).
    • Step 4
  • Figure US20200115354A1-20200416-C00420
    • Rac-trans-1,2,3,4-tetrahydronaphthalene-2,3-diamine
  • Rac-trans-2,3-diazido-1,2,3,4-tetrahydronaphthalene (1.3 g, 2.43 mmol) was dissolved in ethanol (1 mL) and to the solution was added Pd/C (0.5 g, 10% on carbon). The mixture was stirred under H2 (1 atm) overnight until completion. The mixture was filtered through Celite and concentrated in vacuo to give crude rac-trans-1,2,3,4-tetrahydronaphthalene-2,3-diamine (0.82 g, 83% yield) which was used without further purification. 1H NMR (400 MHz, CDCl3) δ 7.10 (ddd, J=16.7, 7.5, 4.3 Hz, 4H), 3.11-3.03 (m, 4H), 2.67 (dd, J=15.9, 10.0 Hz, 2H).
    • Step 5
  • Figure US20200115354A1-20200416-C00421
    • Rac-4-((trans-3-amino-1,2,3,4-tetrahydronaphthalen-2-yl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • 5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2,4-difluoro-N-thiazol-2-yl-benzenesulfonamide (200 mg, 0.43 mmol) was dissolved in DMF (2 mL) and to the solution was added rac-trans-1,2,3,4-tetrahydronaphthalene-2,3-diamine (352 mg, 0.87 mmol) followed by triethylamine (0.18 mL, 1.3 mmol). The reaction mixture was stirred at rt for 4 days until complete. Diluted the reaction with EtOAc (30 mL) and washed with water (10 mL) then brine (2×10 mL). The organic layer was separated and dried over MgSO4, filtered and removed the solvent in vacuo to provide the crude 4-((trans-3-amino-1,2,3,4-tetrahydronaphthalen-2-yl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (220 mg), which was used directly in the next step without further purification.
    • Step 6
  • Figure US20200115354A1-20200416-C00422
    • Rac-5-chloro-N-(2,4-dimethoxybenzyl)-4-((trans-3-(dimethylamino)-1,2,3,4-tetrahydronaphthalen-2-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • To solution of rac-4-((trans-3-amino-1,2,3,4-tetrahydronaphthalen-2-yl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (190 mg, 0.32 mmol) in methanol (2 mL) was added formaldehyde (37% w/w in H2O) (0.23 mL, 3.15 mmol) followed by sodium cyanoborohydride (78 mg, 1.26 mmol). The mixture was stirred at rt overnight. After 16 h, the mixture was concentrated, poured in CH2Cl2 (20 mL) and NaHCO3 (20 mL, saturated). The phases were separated and the CH2Cl2 phases were dried over Na2SO4, filtered and concentrated in vacuo. Purification by flash chromatography through Si gel (20-100% EtOAc/hexanes+1% Et3N) provided rac-5-chloro-N-(2,4-dimethoxybenzyl)-4-((trans-3-(dimethylamino)-1,2,34-tetrahydronaphthalen-2-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide as a mixture of trans enantiomers (123 mg, 62% yield). LCMS (ESI) m/z: 631.2 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 7.75 (d, J=7.1 Hz, 1H), 7.39 (d, J=3.6 Hz, 1H), 7.23-7.08 (m, 5H), 6.96 (d, J=3.6 Hz, 1H), 6.38 (dt, J=12.9, 7.5 Hz, 4H), 5.21 (d, J=2.0 Hz, 2H), 3.75 (s, 6H), 3.49-3.39 (m, 2H), 2.96 (dd, J=45.0, 22.1 Hz, 3H), 2.71 (s, 1H), 2.31 (s, 6H).
    • Step 7
  • Figure US20200115354A1-20200416-C00423
    • 5-Chloro-N-(2,4-dimethoxybenzyl)-4-(((2S,3S)-3-(dimethylamino)-1,2,3,4-tetrahydronaphthalen-2-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((2R,3R)-3-(dimethylamino)-1,2,3,4-tetrahydronaphthalen-2-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Racemic 5-chloro-N-(2,4-dimethoxybenzyl)-4-((trans-3-(dimethylamino)-1,2,3,4-tetrahydronaphthalen-2-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (120 mg, 0.190 mmol) was separated by using chiral HPLC (Chiralpak IB (250 mm×20 mm, 5 um), MeOH:DCM:hexane 5:15:85 containing 0.1% of diethylamine, 15 ml/min) to give 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((2R,3R)-3-(dimethylamino)-1,2,3,4-tetrahydronaphthalen-2-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (1st peak, 42 mg, 35% yield) as white solid. LCMS (ESI) m/z: 631.2 [M+H]+. Also afforded was 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((2S,3S)-3-(dimethylamino)-1,2,3,4-tetrahydronaphthalen-2-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (2nd peak, 43 mg, 36% yield). LCMS (ESI) m/z: 631.2 [M+H]+. Absolute configuration was arbitrarily assigned to each isomer.
    • Step 8
  • Figure US20200115354A1-20200416-C00424
    • 5-Chloro-4-(((2R,3R)-3-(dimethylamino)-1,2,3,4-tetrahydronaphthalen-2-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • 5-Chloro-N-(2,4-dimethoxybenzyl)-4-(((2R,3R)-3-(dimethylamino)-1,2,3,4-tetrahydronaphthalen-2-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (42 mg, 0.07 mmol) was dissolved in 1,4-dioxane (0.50 mL) and to the solution was added formic acid (0.42 mL, 6.65 mmol). The reaction was stirred at rt for 2 h then directly purified by C18 reverse phase flash chromatography (MeCN/10 mM aqueous ammonium bicarbonate, pH=10) to afford 5-chloro-4-(((2R,3R)-3-(dimethylamino)-1,2,3,4-tetrahydronaphthalen-2-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (31 mg, 97% yield). LCMS (ESI) m/z: 481.1 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 7.60 (d, J=7.3 Hz, 1H), 7.22 (d, J=4.5 Hz, 1H), 7.18-7.06 (m, 5H), 6.87 (d, J=12.9 Hz, 1H), 6.77 (d, J=4.5 Hz, 1H), 6.14 (d, J=4.7 Hz, 1H), 3.76 (d, J=8.3 Hz, 2H), 3.19 (s, 1H), 2.87 (dt, J=27.0, 13.4 Hz, 2H), 2.64 (d, J=15.5 Hz, 1H), 2.28 (s, 6H).
    • Example 165
  • Figure US20200115354A1-20200416-C00425
    • 5-Chloro-4-(((2S,3S)-3-(dimethylamino)-1,2,3,4-tetrahydronaphthalen-2-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • 5-Chloro-N-(2,4-dimethoxybenzyl)-4-(((2S,3S)-3-(dimethylamino)-1,2,3,4-tetrahydronaphthalen-2-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (43 mg, 0.07 mmol) dissolved in 1,4-dioxane (0.50 mL) and to the solution was added formic acid (0.42 mL, 6.65 mmol). The reaction was stirred at rt for 2 h, then directly purified by C18 reverse phase flash chromatography (MeCN/10 mM aqueous ammonium bicarbonate, pH=10) to afford 5-chloro-4-(((2S,3S)-3-(dimethylamino)-1,2,3,4-tetrahydronaphthalen-2-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (31 mg, 95% yield). LCMS (ESI) m/z: 481.1 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 7.60 (d, J=7.3 Hz, 1H), 7.22 (d, J=4.5 Hz, 1H), 7.18-7.06 (m, 5H), 6.87 (d, J=12.9 Hz, 1H), 6.77 (d, J=4.5 Hz, 1H), 6.14 (d, J=4.7 Hz, 1H), 3.76 (d, J=8.3 Hz, 2H), 3.19 (s, 1H), 2.87 (dt, J=27.0, 13.4 Hz, 2H), 2.64 (d, J=15.5 Hz, 1H), 2.28 (s, 6H).
    • Example 166
  • Figure US20200115354A1-20200416-C00426
    • N-((1S,2S)-2-((2-Chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)-N-methylacetamide
  • Figure US20200115354A1-20200416-C00427
    • Step 1
  • Figure US20200115354A1-20200416-C00428
    • N-((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)-N-methylacetamide
  • 5-Chloro-N-[(2,4-dimethoxyphenyl)methyl]-2-fluoro-4-[[(1S,2S)-2-(methylamino)cyclohexyl]amino]-N-thiazol-2-yl-benzenesulfonamide (80 mg, 0.14 mmol) was dissolved in CH2Cl2 (1 mL) and to the solution was added pyridine (0.11 mL, 1.41 mmol) followed by acetic anhydride (26 uL, 0.28 mmol). The reaction is stirred at rt for 20 min then directly purified by flash chromatography through Si gel (EtOAc/hexanes) to provide N-((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)-N-methylacetamide (81 mg, 94% yield). LCMS (ESI) m/z: 611.1 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00429
    • N-((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)-N-methylacetamide
  • Following the procedure in Example 165 and making non-critical modifications to substitute 5-Chloro-N-(2,4-dimethoxybenzyl)-4-(((2S,3S)-3-(dimethylamino)-1,2,3,4-tetrahydronaphthalen-2-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with N-((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)-N-methylacetamide (81 mg, 0.13 mmol), N-((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)-N-methyl-acetamide (53 mg, 88% yield) was obtained. LCMS (ESI) m/z: 461.0 [M+H]+. H NMR (400 MHz, d6-DMSO) δ 12.74 (s, 1H), 7.52 (t, J=7.3 Hz, 1H), 7.24 (dd, J=4.6, 0.9 Hz, 1H), 6.79 (qd, J=23.0, 13.3 Hz, 2H), 3.66 (d, J=50.7 Hz, 1H), 2.52 (s, 3H), 2.05 (s, 1H), 2.00 (d, J=11.3 Hz, 1H), 1.87 (s, 2H), 1.82 (s, 1H), 1.67 (dd, J=31.8, 19.8 Hz, 3H), 1.43-1.15 (m, 3H).
    • Example 167
  • Figure US20200115354A1-20200416-C00430
    • N-((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)-2-(dimethylamino)-N-methylacetamide
  • Following the synthetic sequence described in Example 166 and making non-critical modifications to substitute acetic anhydride with 2-(dimethylamino)acetyl chloride, N-((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)-2-(dimethylamino)-N-methylacetamide was obtained. LCMS (ESI) m/z: 504.0 [M+H]+. 1H NMR (400 MHz, d6DMSO) δ 8.13 (s, 1H), 7.51 (dd, J=7.4, 4.1 Hz, 1H), 7.16 (dd, J=4.4, 2.1 Hz, 1H), 6.86-6.68 (m, 2H), 5.91-5.28 (m, 1H), 3.73 (t, J=13.4 Hz, 2H), 3.44 (dd, J=61.1, 14.0 Hz, 1H), 3.26-3.10 (m, 1H), 2.68 (d, J=82.7 Hz, 3H), 2.33 (s, 3H), 2.18 (s, 3H), 1.94 (s, 1H), 1.74-1.53 (m, 4H), 1.31 (s, 3H).
    • Example 168
  • Figure US20200115354A1-20200416-C00431
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-((S)-3-(hydroxymethyl)piperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the synthetic sequence described in Example 70 and making non-critical variations to substitute piperidine with (S)-piperidin-3-ylmethanol, 5-chloro-2-fluoro-4-(((1S,2S)-2-((S)-3-(hydroxymethyl)piperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide formate was obtained. LCMS (ESI) m/z: 503.0 [M+H]+. 1H NMR (400 MHz, d6-dmso) δ 8.14 (s, 1H), 7.59 (d, J=7.3 Hz, 1H), 7.22 (d, J=4.5 Hz, 1H), 6.77 (d, J=4.5 Hz, 1H), 6.68 (d, J=12.8 Hz, 1H), 6.04 (s, 1H), 3.24-3.12 (m, 3H), 2.66-2.58 (m, 1H), 2.55-2.51 (m, 2H), 2.47-2.36 (m, 1H), 2.28-2.16 (m, 1H), 1.94-1.71 (m, 3H), 1.66-1.49 (m, 3H), 1.47-1.30 (m, 3H), 1.27-1.17 (m, 2H), 1.15-1.02 (m, 1H), 0.99-0.84 (m, 1H).
    • Example 169
  • Figure US20200115354A1-20200416-C00432
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-((R)-3-(hydroxymethyl)piperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the synthetic sequence described in Example 70 and making non-critical variations to substitute piperidine with (R)-piperidin-3-ylmethanol, 5-chloro-2-fluoro-4-(((1S,2S)-2-((R)-3-(hydroxymethyl)piperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide formate was obtained. LCMS (ESI) m/z: 503.1 [M+H]+. 1H NMR (400 MHz, d6-dmso) δ 8.14 (s, 1H), 7.59 (d, J=7.3 Hz, 1H), 7.22 (d, J=4.5 Hz, 1H), 6.77 (d, J=4.5 Hz, 1H), 6.67 (d, J=12.8 Hz, 1H), 6.00 (s, 1H), 4.38 (s, 1H), 3.27 (dd, J=10.6, 5.6 Hz, 1H), 3.24-3.17 (m, 1H), 3.14 (dd, J=10.6, 7.2 Hz, 1H), 2.75-2.69 (m, 1H), 2.57-2.52 (m, 1H), 2.47-2.42 (m, 1H), 2.25-2.10 (m, 2H), 1.99 (t, J=10.5 Hz, 1H), 1.87-1.72 (m, 2H), 1.67-1.30 (m, 5H), 1.29-1.04 (m, 4H), 0.91-0.76 (m, 1H).
    • Example 170
  • Figure US20200115354A1-20200416-C00433
    • 4-(((1S,2S,4S)-2-(azetidin-3-yl(methyl)amino)-4-(trifluoromethyl)cyclohexyl)amino)-5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide Step 1
  • Figure US20200115354A1-20200416-C00434
    • benzyl 3-(((1S,6S)-6-((tert-butoxycarbonyl)amino)-3-(trifluoromethyl)cyclohex-3-en-1-yl)(methyl)amino)azetidine-1-carboxylate
  • Following the procedure described in Example 138, step 1, step 2 and making non-critical variations as required to replace (tert-butyldimethylsilyloxy)acetaldehyde with benzyl 3-oxoazetidine-1-carboxylate, benzyl 3-(((1S,6S)-6-((tert-butoxycarbonyl)amino)-3-(trifluoromethyl)cyclohex-3-en-1-yl)(methyl)amino)azetidine-1-carboxylate was obtained as a light yellow solid. 1H NMR (400 MHz, CDCl3) δ 7.50-7.31 (m, 5H), 6.22 (s, 1H), 5.24 (s, 1H), 5.11 (s, 2H), 4.05-4.00 (m, 2H), 3.99-3.91 (m, 2H), 3.69-3.65 (m, 1H), 3.64-3.45 (m, 1H), 3.15-3.01 (m, 1H), 2.71-2.66 (m, 1H), 2.31-2.10 (m, 2H), 2.19 (s, 3H), 2.01-1.98 (m, 1H), 1.45 (s, 9H).
    • Step 2
  • Figure US20200115354A1-20200416-C00435
    • benzyl 3-(((1S,6S)-6-((2-chloro-5-fluoro-4-(N-(6-fluoropyridin-2-yl)-N-(methoxymethyl)sulfamoyl)phenyl)amino)-3-(trifluoromethyl)cyclohex-3-en-1-yl)(methyl)amino)azetidine-1-carboxylate
  • Following the procedure described in Example 138, step 4, step 5 and making non-critical variations as required to replace tert-butyl((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)carbamate and 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide with benzyl 3-(((1S,6S)-6-((tert-butoxycarbonyl)amino)-3-(trifluoromethyl)cyclohex-3-en-1-yl)(methyl)amino)azetidine-1-carboxylate and 5-chloro-2,4-difluoro-N-(6-fluoropyridin-2-yl)-N-(methoxymethyl)benzenesulfonamide, benzyl 3-(((1S,6S)-6-((2-chloro-5-fluoro-4-(N-(6-fluoropyridin-2-yl)-N-(methoxymethyl)sulfamoyl)phenyl)amino)-3-(trifluoromethyl)cyclohex-3-en-1-yl)(methyl)amino)azetidine-1-carboxylate was obtained as a light yellow solid. LCMS (ESI) m/z: 752.0 [M+Na]+.
    • Step 3
  • Figure US20200115354A1-20200416-C00436
    • 4-(((1S,6S)-6-(azetidin-3-yl(methyl)amino)-4-(trifluoromethyl)cyclohex-3-en-1-yl)amino)-5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-N-(methoxymethyl)benzenesulfonamide
  • Following the procedure described in Example 138, step 3 and making non-critical variations as required to replace tert-butyl((1S,6S)-6-((2-((tert-butyldimethylsilyl)oxy)ethyl)(methyl)amino)-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate with benzyl 3-(((1S,6S)-6-((2-chloro-5-fluoro-4-(N-(6-fluoropyridin-2-yl)-N-(methoxymethyl)sulfamoyl)phenyl)amino)-3-(trifluoromethyl)cyclohex-3-en-1-yl)(methyl)amino)azetidine-1-carboxylate, 4-(((1S,6S)-6-(azetidin-3-yl(methyl)amino)-4-(trifluoromethyl)cyclohex-3-en-1-yl)amino)-5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-N-(methoxymethyl)benzenesulfonamide was obtained as a yellow solid. LCMS (ESI) m/z: 598.1 [M+H]+.
    • Step 4
  • Figure US20200115354A1-20200416-C00437
    • 4-(((1S,2S,4S)-2-(azetidin-3-yl(methyl)amino)-4-(trifluoromethyl)cyclohexyl)amino)-5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide
  • Following the procedure described in Example 138, step 6 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S,4S)-2-((2-hydroxyethyl)(methyl)amino)-4-(trifluoromethyl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide with 4-(((1S,6S)-6-(azetidin-3-yl(methyl)amino)-4-(trifluoromethyl)cyclohex-3-en-1-yl)amino)-5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)-N-(methoxymethyl)benzenesulfonamide, 4-(((1S,2S,4S)-2-(azetidin-3-yl(methyl)amino)-4-(trifluoromethyl)cyclohexyl)amino)-5-chloro-2-fluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide was obtained as a light yellow solid. LCMS (ESI) m/z: 554.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.21 (s, 1H), 7.20 (d, J=7.2 Hz, 1H), 7.49-7.42 (m, 1H), 6.71 (d, J=13.2 Hz, 1H), 6.59-6.53 (m, 1H), 6.19-6.16 (m, 1H), 5.60 (d, J=7.6 Hz, 1H), 3.95-3.91 (m, 1H), 3.86-3.78 (m, 4H), 2.83-2.76 (m, 1H), 2.29-2.23 (m, 1H), 2.14 (s, 3H), 2.04-2.01 (m, 1H), 1.81-1.75 (m, 2H), 1.39-1.23 (m, 4H).
    • Example 171
  • Figure US20200115354A1-20200416-C00438
    • 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(4-methylthiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 112 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(4-methylthiazol-2-yl)benzenesulfonamide, 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(4-methylthiazol-2-yl)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 515.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.14 (s, 1H), 7.57 (d, J=7.2 Hz, 1H), 6.82 (d, J=13.2 Hz, 1H), 6.37 (d, J=1.2 Hz, 1H), 5.82 (d, J=5.2 Hz, 1H), 3.52-3.48 (m, 1H), 2.86-2.75 (m, 1H), 2.45-2.34 (m, 1H), 2.19 (s, 3H), 2.19 (s, 3H), 2.18-2.11 (m, 1H), 2.08 (s, 3H), 2.03-1.95 (m, 1H), 1.84-1.73 (m, 1H), 1.59-1.44 (m, 1H), 1.35-1.19 (m, 2H).
    • Example 172
  • Figure US20200115354A1-20200416-C00439
    • 5-Chloro-N-(5-chloro-4-methylthiazol-2-yl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluorobenzenesulfonamide
  • Figure US20200115354A1-20200416-C00440
    • Step 1
  • Figure US20200115354A1-20200416-C00441
    • 5-Chloro-N-(5-chloro-4-methylthiazol-2-yl)-2,4-difluorobenzenesulfonamide
  • To a solution of 5-chloro-2,4-difluorobenzenesulfonylchloride (150 mg, 0.61 mmol) in CH2Cl2 (2 mL) and pyridine (1 mL) was added 5-chloro-4-methyl-thiazol-2-amine (108 mg, 0.73 mmol) and the mixture stirred at rt overnight. After 16 h, the reaction mixture was diluted with CH2Cl2 (50 mL), washed with saturated aqueous NaHCO3 (10 mL), dried (Na2SO4), filtered and concentrated in vacuo to remove pyridine. The crude residue was purified by flash chromatography through Si gel (0-100% EA/CH2Cl2) to provide 5-chloro-N-(5-chloro-4-methyl-thiazol-2-yl)-2,4-difluoro-benzenesulfonamide (86 mg, 39%) as a yellow oil. LCMS (ESI) m/z: 358.9, 360.9 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00442
    • 5-Chloro-N-(5-chloro-4-methylthiazol-2-yl)-N-(ethoxymethyl)-2,4-difluorobenzenesulfonamide
  • To a solution of 5-chloro-N-(5-chloro-4-methyl-thiazol-2-yl)-2,4-difluoro-benzenesulfonamide (80 mg, 0.22 mmol) in CH2Cl2 (0.89 mL) was added chloromethyl ethyl ether (0.02 mL, 0.27 mmol), followed by DIPEA (0.06 mL, 0.33 mmol). The reaction mixture was stirred at 25° C. for 16 h. The reaction was diluted CH2Cl2 (5 mL) and saturated aqueous NaHCO3 (10 mL) and the phases were separated. Organic extract was dried with Na2SO4 and evaporated. The residue was purified by flash chromatography through Si gel (100% CH2Cl2) to provide 5-chloro-N-(5-chloro-4-methyl-thiazol-2-yl)-N-(ethoxymethyl)-2,4-difluoro-benzenesulfonamide (33 mg, 36% yield). LCMS (ESI) m/z: 416.9, 418.9 [M+H]+.
    • Step 3
  • Figure US20200115354A1-20200416-C00443
    • 5-Chloro-N-(5-chloro-4-methylthiazol-2-yl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-N-(ethoxymethyl)-2-fluorobenzenesulfonamide
  • Following the procedure described in Example 141 step 1 and making non-critical variations, (1S,2S)—N1,N1-dimethylcyclohexane-1,2-diamine was used to afford 5-chloro-N-(5-chloro-4-methylthiazol-2-yl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-N-(ethoxymethyl)-2-fluorobenzenesulfonamide (30 mg, 73%). LCMS (ESI) m/z: 539.0, 541.0, 543.0 [M+H]+.
    • Step 4
  • Figure US20200115354A1-20200416-C00444
    • 5-Chloro-N-(5-chloro-4-methylthiazol-2-yl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluorobenzenesulfonamide
  • Following the procedure described in Example 115 step 2 and making non-critical variations using (1S,2S)—N1,N1-dimethylcyclohexane-1,2-diamine and substituting TFA for formic acid, 5-chloro-N-(5-chloro-4-methylthiazol-2-yl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-N-(ethoxymethyl)-2-fluorobenzenesulfonamide, 5-chloro-N-(5-chloro-4-methylthiazol-2-yl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluorobenzenesulfonamide was obtained. LCMS (ESI) m/z: 481.0 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 7.52 (d, J=7.2 Hz, 1H), 6.78 (s, 1H), 5.75 (s, 1H), 2.51-2.32 (m, 4H), 2.08 (s, J=42.2 Hz, 7H), 1.80 (d, J=10.4 Hz, 1H), 1.62 (d, J=12.2 Hz, 1H), 1.46-1.12 (m, 6H).
    • Example 173 & Example 174
  • Figure US20200115354A1-20200416-C00445
    • 5-chloro-4-(((1S,2S,5R)-2-(dimethylamino)-5-(2-fluorophenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1S,2S,5S)-2-(dimethylamino)-5-(2-fluorophenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Step 1
  • Figure US20200115354A1-20200416-C00446
    • tert-butyl((3S,4S)-3-amino-2′-fluoro-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate
  • Following the procedure described in Example 23 and making non-critical variations as required to replace 2-(trifluoromethyl)allyl 4-methylbenzenesulfonate with 1-fluoro-2-(3-iodoprop-1-en-2-yl)benzene, tert-butyl((3S,4S)-3-amino-2′-fluoro-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate was obtained as a white solid.
    • Step 2
  • Figure US20200115354A1-20200416-C00447
    • 5-chloro-4-(((1S,2S,5R)-2-(dimethylamino)-5-(2-fluorophenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1S,2S,5S)-2-(dimethylamino)-5-(2-fluorophenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 88 and making non-critical variations as required to replace (trans)-tert-butyl(3-amino-3′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate with tert-butyl((3S,4S)-3-amino-2′-fluoro-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate, 5-chloro-4-(((1S,2S,5R)-2-(dimethylamino)-5-(2-fluorophenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (first peak on HPLC) and 5-chloro-4-(((1S,2S,5S)-2-(dimethylamino)-5-(2-fluorophenyl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (second peak on HPLC) were obtained both as white solid. Example 173: LCMS (ESI) m/z: 527.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.57 (d, J=7.2 Hz, 1H), 7.30-7.26 (m, 1H), 7.24-7.20 (m, 1H), 7.17 (d, J=4.4 Hz 1H), 7.15-7.10 (m, 2H), 6.83 (d, J=13.2 Hz, 1H), 6.73 (d, J=4.4 Hz, 1H), 5.87 (d, J=6.0 Hz, 1H), 3.73-3.65 (m, 1H), 3.17-3.06 (m, 1H), 2.96-2.88 (m, 1H), 2.30 (s, 3H), 2.30 (s, 3H), 2.14-2.11 (m, 1H), 2.00-1.90 (m, 2H), 1.61-1.55 (m, 1H), 1.53-1.45 (m, 2H). Example 174: LCMS (ESI) m/z: 527.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.62 (d, J=7.2 Hz, 1H), 7.43-7.39 (m, 1H), 7.29-7.24 (m, 1H), 7.22-7.20 (m, 1H), 7.18-7.16 (m, 1H), 7.14-7.11 (m, 1H), 6.76 (d, J=4.4 Hz, 1H), 6.54 (d, J=12.8 Hz, 1H), 5.56 (d, J=6.4 Hz, 1H), 3.85-3.83 (m, 1H), 3.24-3.22 (m, 1H), 2.45-2.43 (m, 1H), 2.30 (s, 3H), 2.30 (s, 3H), 2.24-2.18 (m, 1H), 1.89-1.80 (m, 3H), 1.73-1.64 (m, 2H).
    • Example 175
  • Figure US20200115354A1-20200416-C00448
    • 5-chloro-2-fluoro-4-(((1S,2S)-2-(methyl(1-(2,2,2-trifluoroethyl)azetidin-3-yl)amino)cyclohexyl)-amino)-N-(thiazol-2-yl)benzenesulfonamide Step 1
  • Figure US20200115354A1-20200416-C00449
    • 5-Chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(methyl(1-(2,2,2-trifluoroethyl)azetidin-3-yl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • 4-(((1S,2S)-2-(Azetidin-3-yl(methyl)amino)cyclohexyl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (60 mg, 0.10 mmol) was dissolved in DMF (0.50 mL) and THF (0.50 mL). To the solution was added DIPEA (49.6 mg, 0.38 mmol) followed by 2,2,2-trifluoroethyl trifluoromethanesulfonate (33 mg, 0.14 mmol). The reaction is stirred at r.t. for 2 hours till completion. Dilute the reaction with EtOAc (30 mL) and wash it with brine (3×10 mL). The organic layer was dried over Na2SO4 and the solvent was removed in vacuo. The crude mixture was purified by flash column chromatography (silica, EtOAc and hexanes as eluent) to 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((S, 2S)-2-(methyl(1-(2,2,2-trifluoroethyl)azetidin-3-yl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (15 mg, 22% yield). LCMS (ESI) m/z: 706.2 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00450
    • 5-Chloro-2-fluoro-4-(((1S,2S)-2-(methyl(1-(2,2,2-trifluoroethyl)azetidin-3-yl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Dissolve 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-(methyl(1-(2,2,2-trifluoroethyl)azetidin-3-yl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (15 mg, 0.02 mmol) in 1,4-dioxane (0.5 mL) and to the solution was added formic acid (0.50 mL). The reaction was stirred at rt for 2 hours until completion. The reaction was concentrated to dryness and purified by reverse phase flash column chromatography on a C18 column (MeCN/10 mM aqueous ammonium formate) to afford 5-chloro-2-fluoro-4-(((1S,2S)-2-(methyl(1-(2,2,2-trifluoroethyl)azetidin-3-yl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (7 mg, 60% yield). LCMS (ESI) m/z: 556.0 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 8.12 (s, 1H), 7.56 (d, J=7.3 Hz, 1H), 7.15 (d, J=4.3 Hz, 1H), 6.68 (t, J=9.1 Hz, 2H), 5.68 (d, J=4.9 Hz, 1H), 3.43 (dd, J=17.6, 13.2 Hz, 3H), 3.07 (q, J=10.2 Hz, 2H), 2.94 (t, J=6.5 Hz, 1H), 2.81 (t, J=6.1 Hz, 1H), 2.41 (d, J=10.5 Hz, 1H), 2.05 (s, 1H), 1.61 (dd, J=34.4, 12.9 Hz, 3H), 1.19 (dd, J=39.9, 30.8 Hz, 4H).
    • Example 176
  • Figure US20200115354A1-20200416-C00451
    • 5-Chloro-4-(((1S,2S)-2-((R)-3-cyanopiperidin-1-yl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the synthetic sequence described in Example 70 and making non-critical variations to substitute piperidine with rac-piperidine-3-carbonitrile, the title compound was obtained as a mixture of diastereomers. Separation of the isomers by HPLC gave 5-Chloro-4-(((1S,2S)-2-((R)-3-cyanopiperidin-1-yl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide as a white solid. The stereochemistry of the nitrile group was arbitrarily assigned. LCMS (ESI) m/z: 497.9 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.40 (br s, 1H), 7.52 (d, J=7.3 Hz, 1H), 7.13 (d, J=4.3 Hz, 1H), 6.67 (d, J=4.3 Hz, 1H), 6.62 (d, J=12.8 Hz, 1H), 5.71 (d, J=3.2 Hz, 1H), 2.82-2.73 (m, 1H), 2.64 (d, J=4.7 Hz, 2H), 2.59 (t, J=10.1 Hz, 1H), 2.44-2.38 (m, 1H), 2.38-2.29 (m, 1H), 2.11 (d, J=11.1 Hz, 1H), 1.79 (d, J=7.2 Hz, 1H), 1.72 (d, J=5.0 Hz, 1H), 1.68-1.45 (m, 4H), 1.41-1.14 (m, 5H), 1.08 (q, J=11.7 Hz, 1H).
    • Example 177
  • Figure US20200115354A1-20200416-C00452
    • 5-chloro-4-(((1S,2S)-2-((S)-3-cyanopiperidin-1-yl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the synthetic sequence described in Example 70 and making non-critical variations to substitute piperidine with rac-piperidine-3-carbonitrile, the title compound was obtained as a mixture of diastereomers. Separation of the isomers by HPLC gave 5-Chloro-4-(((1S,2S)-2-((S)-3-cyanopiperidin-1-yl)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide as a white solid. The stereochemistry of the nitrile group was arbitrarily assigned. LCMS (ESI) m/z: 498.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.76 (s, 1H), 7.57 (d, J=7.3 Hz, 1H), 7.24 (d, J=4.6 Hz, 1H), 6.79 (d, J=4.6 Hz, 1H), 6.69 (d, J=13.0 Hz, 1H), 5.92 (d, J=3.4 Hz, 1H), 2.79 (s, 1H), 2.73-2.55 (m, 3H), 2.21 (t, J=8.0 Hz, 1H), 2.14 (d, J=11.3 Hz, 1H), 1.82-1.67 (m, 2H), 1.67-1.48 (m, 3H), 1.48-1.27 (m, 3H), 1.27-1.05 (m, 3H). 2 CH under the solvent or HOD signal.
    • Example 178
  • Figure US20200115354A1-20200416-C00453
    • 5-cyclopropyl-4-(((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 15 and making non-critical variations as required to replace 5-bromo-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide, 5-cyclopropyl-4-(((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 502.4 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.56 (s, 1H), 8.29 (d, J=4.8 Hz, 1H), 7.39 (d, J=8.0 Hz, 1H), 6.92 (d, J=5.6 Hz, 1H), 6.56 (d, J=13.2 Hz, 1H), 5.99 (s, 1H), 3.43-3.42 (m, 1H), 2.90-2.80 (m, 1H), 2.24 (s, 3H), 2.24 (s, 3H), 2.20-2.15 (m, 1H), 2.09-1.97 (m, 1H), 1.85-1.76 (m, 1H), 1.63-1.44 (m, 2H), 1.43-1.15 (m, 3H), 0.95-0.80 (m, 2H), 0.65-0.55 (m, 1H), 0.35-0.25 (m, 1H).
    • Example 179
  • Figure US20200115354A1-20200416-C00454
    • N-((1S,2S)-2-((2-Chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)-N,1-dimethylazetidine-3-carboxamide
  • Following the synthetic sequence described in Example 166 and making non-critical modifications to substitute acetic anhydride with 1-methylazetidine-3-carboxylic acid and add HATU, N-((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)-cyclohexyl)-2-(dimethylamino)-N-methylacetamide was obtained. LCMS (ESI) m/z: 516.0 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 7.87 (d, J=7.4 Hz, 1H), 6.99 (d, J=4.4 Hz, 1H), 6.48 (d, J=4.4 Hz, 1H), 6.25 (d, J=12.4 Hz, 1H), 5.01 (br s, 1H), 4.64-4.45 (m, 1H), 3.91 (t, J=8.2 Hz, 2H), 3.74-3.46 (m, 2H), 3.39-3.18 (m, 1H), 2.78-2.56 (m, 1H), 2.67 (s, 3H), 2.47 (s, 3H), 2.25-2.14 (m, 1H), 1.93-1.66 (m, 3H), 1.49-1.21 (m, 4H).
    • Example 180
  • Figure US20200115354A1-20200416-C00455
    • 5-chloro-4-(((1S,2S,4S)-2-(cyclobutyl(methyl)amino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 138 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide and (tert-butyldimethylsilyloxy)acetaldehyde with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide and cyclobutanone, 5-chloro-4-(((1S,2S,4S)-2-(cyclobutyl(methyl)amino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 536.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.56 (s, 1H), 8.27 (d, J=6.0 Hz, 1H), 7.72 (d, J=7.6 Hz, 1H), 6.92 (d, J=6.0 Hz, 1H), 6.81 (d, J=13.2 Hz, 1H), 5.86 (d, J=4.8 Hz, 1H), 3.42-3.39 (m, 1H), 3.25-3.14 (m, 1H), 2.83-2.73 (m, 1H), 2.43-2.35 (m, 1H), 2.20-2.11 (m, 1H), 1.99 (s, 3H), 1.94-1.84 (m, 2H), 1.82-1.62 (m, 3H), 1.60-1.41 (m, 3H), 1.39-1.21 (m, 2H).
    • Example 181
  • Figure US20200115354A1-20200416-C00456
    • 5-chloro-4-[[(1S,2S,4S)-2-(dimethylamino)-4-[3-(trifluoromethyl)phenyl]cyclohexyl]amino]-2-fluoro-N-pyrimidin-4-yl-benzenesulfonamide
  • Following the procedure described in Example 224 and making non-critical variations as required to replace 1-(4-(trifluoromethyl)phenyl)ethanone with 1-(3-(trifluoromethyl)phenyl)ethanone, 5-chloro-4-[[(1S,2S,4S)-2-(dimethylamino)-4-[3-(trifluoromethyl)phenyl]cyclohexyl]amino]-2-fluoro-N-pyrimidin-4-yl-benzenesulfonamide was obtained. LCMS (ESI) m/z: 572.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.37 (s, 1H), 8.15 (s, 1H), 8.03 (d, J=6.0 Hz, 1H), 7.68-7.60 (m, 3H), 7.58-7.55 (m, 2H), 6.75 (d, J=12.8 Hz, 1H), 6.66 (d, J=6.0 Hz, 1H), 5.88 (d, J=6.4 Hz, 1H), 3.65-3.63 (m, 1H), 3.20-3.15 (m, 1H), 2.85-2.80 (m, 1H), 2.39 (s, 3H), 2.39 (s, 3H), 2.16-2.11 (m, 1H), 2.10-2.01 (m, 1H), 1.78-1.73 (m, 2H), 1.69-1.58 (m, 1H), 1.41-1.37 (m, 1H).
    • Example 182 & Example 183
  • Figure US20200115354A1-20200416-C00457
    • 5-chloro-4-(((1R,2R)-2-(6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide & 5-chloro-4-(((1S,2S)-2-(6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 98 & Example 99 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide and pyrrolidine, with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide and 6,6-difluoro-3-azabicyclo[3.1.0]hexane, racemic (trans)-5-chloro-4-((2-(6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide was obtained as a white solid. Racemic (trans)-5-chloro-4-((2-(6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide was separated by chiral SFC ((Amylose-1 (150 mm*21.2 mm, 10 um), Supercritical CO2/MeOH+0.1% NH4OH=60/40; 70 mL/min) to give 5-chloro-4-(((1R,2R)-2-(6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (first peak) and 5-chloro-4-(((1S,2S)-2-(6,6-difluoro-3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (second peak) as white solids. Absolute configuration was arbitrarily assigned to each enantiomer. Example 182: LCMS (ESI) m/z: 502.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.60 (s, 1H), 8.32 (s, 1H), 7.70 (d, J=7.3 Hz, 1H), 6.99 (d, J=6.3 Hz, 1H), 6.70 (d, J=13.2 Hz, 1H), 5.79 (d, J=5.1 Hz, 1H), 3.28-3.17 (m, 1H), 3.10-2.97 (m, 2H), 2.94-2.83 (m, 1H), 2.82-2.68 (m, 2H), 2.29-2.13 (m, 2H), 2.14-1.98 (m, 1H), 1.86-1.69 (m, 2H), 1.66-1.51 (m, 1H), 1.42-1.18 (m, 3H), 1.15-1.03 (m, 1H). Example 183: LCMS (ESI) m/z: 502.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.60 (s, 1H), 8.32 (s, 1H), 7.70 (d, J=7.3 Hz, 1H), 6.99 (d, J=6.2 Hz, 1H), 6.70 (d, J=13.2 Hz, 1H), 5.79 (s, 1H), 3.27-3.23 (m, 1H), 3.11-2.97 (m, 2H), 2.93-2.84 (m, 1H), 2.81-2.69 (m, 2H), 2.28-2.14 (m, 2H), 2.15-1.99 (m, 1H), 1.84-1.68 (m, 2H), 1.66-1.52 (m, 1H), 1.45-1.17 (m, 3H), 1.16-1.02 (m, 1H).
    • Example 184 & Example 185
  • Figure US20200115354A1-20200416-C00458
    • 5-chloro-2-fluoro-4-(((1R,2R)-2-((1R,5S,6R)-6-(hydroxymethyl)-3-azabicyclo-[3.1.0]hexan-3-yl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide & 5-chloro-2-fluoro-4-(((1S,2S)-2-((1R,5S,6R)-6-(hydroxymethyl)-3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 98 & Example 99 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(6-fluoropyridin-2-yl)benzenesulfonamide and pyrrolidine, with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide and ((1R,5S,6R)-3-azabicyclo[3.1.0]hexan-6-yl)methanol, racemic (trans)-5-chloro-2-fluoro-4-((2-((1R,5S,6R)-6-(hydroxymethyl)-3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide was obtained as a white solid. Racemic (trans)-5-chloro-2-fluoro-4-((2-((1R,5S,6R)-6-(hydroxymethyl)-3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide was separated by chiral SFC ((Chiralpak IC (150 mm*21.2 mm, 10 um), Supercritical CO2/MeOH=55/45; 70 mL/min) to give 5-chloro-2-fluoro-4-(((1R,2R)-2-((1R,5S,6R)-6-(hydroxymethyl)-3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide (first peak) and 5-chloro-2-fluoro-4-(((1S,2S)-2-((1R,5S,6R)-6-(hydroxymethyl)-3-azabicyclo[3.1.0]hexan-3-yl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide (second peak) as white solids. Absolute configuration was arbitrarily assigned to each enantiomer. Example 184: LCMS (ESI) m/z: 513.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 11.42 (s, 1H), 7.83 (q, J=8.2 Hz, 1H), 7.74 (d, J=7.3 Hz, 1H), 6.88 (dd, J=8.0, 2.1 Hz, 1H), 6.77-6.59 (m, 2H), 5.90 (d, J=4.0 Hz, 1H), 4.33 (t, J=5.5 Hz, 1H), 3.24-3.06 (m, 3H), 2.79 (s, 2H), 2.74-2.60 (m, 1H), 2.44-2.36 (m, 1H), 2.20-2.01 (m, 1H), 1.87-1.66 (m, 2H), 1.66-1.51 (m, 1H), 1.48-0.99 (m, 7H). Example 185: LCMS (ESI) m/z: 513.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 11.42 (s, 1H), 7.83 (q, J=8.1 Hz, 1H), 7.74 (d, J=7.3 Hz, 1H), 6.88 (d, J=7.9 Hz, 1H), 6.71 (d, J=13.4 Hz, 2H), 5.90 (s, 1H), 4.33 (t, J=5.5 Hz, 1H), 3.24-3.06 (m, 3H), 2.78 (s, 2H), 2.74-2.61 (m, 2H), 2.44-2.34 (m, 1H), 2.18-2.01 (m, 1H), 1.85-1.67 (m, 2H), 1.64-1.51 (m, 1H), 1.46-1.01 (m, 7H).
    • Example 186
  • Figure US20200115354A1-20200416-C00459
    • 5-Chloro-N-(4-chlorothiazol-2-yl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluorobenzenesulfonamide
  • Following the procedure described in Example 172 and making non-critical variations substituting 5-chloro-4-methyl-thiazol-2-amine with tert-butyl N-(4-chlorothiazol-2-yl)carbamate the title compound was obtained as a white solid. LCMS (ESI) m/z: 466.9, 468.9 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 8.57 (s, 1H), 7.57 (d, J=7.1 Hz, 1H), 6.96 (d, J=11.6 Hz, 1H), 6.35 (s, 1H), 5.87 (d, J=9.9 Hz, 1H), 3.89 (d, J=7.5 Hz, 1H), 3.49 (t, J=8.6 Hz, 1H), 2.74 (s, 3H), 2.60 (s, 3H), 2.16-2.00 (m, 1H), 1.95-1.87 (m, 1H), 1.83 (d, J=12.1 Hz, 1H), 1.63 (d, J=11.5 Hz, 1H), 1.48-1.24 (m, 4H).
    • Example 187
  • Figure US20200115354A1-20200416-C00460
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Figure US20200115354A1-20200416-C00461
    Figure US20200115354A1-20200416-C00462
    • Step 1
  • Figure US20200115354A1-20200416-C00463
    • Rac-di-tert-butyl(1,2-trans)-cyclohex-4-ene-1,2-diyldicarbamate
  • To a suspension of rac-trans-cyclohex-4-ene-1,2-diamine dihydrochloride (500 mg, 2.7 mmol) in CH2Cl2 (10 mL) was added triethylamine (1.37 g, 1.88 mL, 13.5 mmol) followed by addition of di-tert-butyl dicarbonate (1.47 g, 6.75 mmol) and the mixture stirred at rt overnight. After 20 h, the mixture was diluted with EtOAc (50 mL) and washed with saturated NaHCO3(aq) (10 mL), dried (Na2SO4), filtered and concentrated in vacuo. The crude residue thus obtained was purified by flash chromatography through Si gel (0-100% EtOAc/CH2Cl2) to provide rac-di-tert-butyl(1,2-trans)-cyclohex-4-ene-1,2-diyldicarbamate (726 mg, 86%) as a white solid. LCMS (ESI) m/z: 313.3 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 5.65-5.48 (m, 2H), 4.87 (s, 2H), 3.73-3.52 (m, 2H), 2.48 (d, J=16.7 Hz, 2H), 2.05-1.90 (m, 2H), 1.43 (s, 18H).
    • Step 2
  • Figure US20200115354A1-20200416-C00464
    • Rac-di-tert-butyl((1,2-trans)-4-oxocyclohexane-1,2-diyl)dicarbamate
  • A flask containing rac-di-tert-butyl(1,2-trans)-cyclohex-4-ene-1,2-diyldicarbamate (716 mg, 2.29 mmol) was capped and purged with N2 for 5 min. The flask was then charged with THF (5 mL) and cooled to 0° C. before addition of 1M BH3.THF (3.44 mL, 3.44 mmol) and the mixture was stirred overnight in the bath allowing to warm to rt. After 16 h, 1N NaOH (3.44 mL, 3.44 mmol) was cautiously added followed immediately by addition of 33 wt % H2O2(aq) (0.8 mL, 26 mmol) and the resulting mixture stirred at rt. After 1 h, the mixture was diluted with 50% saturated Na2S2O3(aq) (30 mL) and extracted with EtOAc (75 mL). Organic extract was dried (Na2SO4), filtered and concentrated in vacuo to provide rac-di-tert-butyl((1,2-trans)-4-hydroxycyclohexane-1,2-diyl)dicarbamate (753 mg, 99%) as a mixture of alcohol isomers as a white solid. Used directly in the next step without further purification. LCMS (ESI) m/z: 331.3 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 5.27-4.94 (m, 2H), 3.80-3.54 (m, 1H), 3.44-3.19 (m, 2H), 2.83 (br.s, 1H), 2.32-2.06 (m, 1H), 1.99-1.88 (m, 4H), 1.86-1.63 (m, 1H), 1.39 (s, 18H).
  • The crude mixture of rac-di-tert-butyl((1,2-trans)-4-hydroxycyclohexane-1,2-diyl)dicarbamate alcohol isomers prepared above (753 mg, 2.28 mmol) was dissolved in CH2Cl2 (10 mL) and to this was then added Dess-Martin Periodinane (1.16 g, 2.73 mmol) and the mixture stirred at rt overnight. After 16 h, the mixture was diluted with CH2Cl2 (50 mL), washed with saturated NaHCO3(aq) (2×10 mL), dried (Na2SO4), filtered and concentrated in vacuo. Purification by flash chromatography through Si gel (0-100% EtOAc/hexanes) provided rac-di-tert-butyl((1,2-trans)-4-oxocyclohexane-1,2-diyl)dicarbamate (392 mg, 52%) as a tan solid. LCMS (ESI) m/z: 329.4 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 5.08 (d, J=7.5 Hz, 1H), 5.04 (d, J=8.0 Hz, 1H), 3.84-3.62 (m, 2H), 2.71 (dd, J=14.3, 3.5 Hz, 1H), 2.39 (dd, J=10.5, 4.8 Hz, 2H), 2.36-2.26 (m, 1H), 2.26-2.16 (m, 1H), 1.60-1.48 (m, 1H), 1.45-1.36 (m, 18H).
    • Step 3
  • Figure US20200115354A1-20200416-C00465
    • Rac-di-tert-butyl((1,2-trans)-4,4-difluorocyclohexane-1,2-diyl)dicarbamate
  • Rac-di-tert-butyl((1,2-trans)-4-oxocyclohexane-1,2-diyl)dicarbamate (392 mg, 1.19 mmol) dissolved in CH2Cl2 (5 mL) in a N2 flushed flask and cooled to 0° C. Diethylaminosulfur trifluoride (1.91 mL of a 1M sol'n in CH2Cl2, 1.91 mmol) was then added and the mixture stirred allowing to warm slowly to rt. After 3 days, the mixture was diluted with CH2Cl2 (50 mL), washed with H2O (10 mL), then with saturated NaHCO3(aq) (2×10 mL), dried (Na2SO4), filtered and concentrated in vacuo. Purification by flash column chromatography through Si gel (0-50% EtOAc/hexanes) provided rac-di-tert-butyl((1,2-trans)-4,4-difluorocyclohexane-1,2-diyl)dicarbamate (215 mg, 51%) as a white solid. LCMS (ESI) m/z: 351.4 [M+H]+. H NMR (400 MHz, CDCl3) δ 5.18-4.99 (m, 1H), 4.94 (d, J=7.8 Hz, 1H), 3.72-3.58 (m, 1H), 3.53-3.35 (m, 1H), 2.45 (dt, J=29.0, 12.2 Hz, 1H), 2.17-1.94 (m, 2H), 1.88-1.61 (m, 1H), 1.54-1.32 (m, 20H).
    • Step 4
  • Figure US20200115354A1-20200416-C00466
    • Rac-(1,2-trans)-4,4-difluorocyclohexane-1,2-diamine Dihydrochloride
  • To a solution of rac-di-tert-butyl((1,2-trans)-4,4-difluorocyclohexane-1,2-diyl)dicarbamate (215 mg, 0.61 mmol) in EtOAc (3 mL) was added 4N HCl in dioxanes (1 mL, 4 mmol) and the mixture stirred at rt. After 18 h, the mixture was concentrated in vacuo to provide rac-(1,2-trans)-4,4-difluorocyclohexane-1,2-diamine dihydrochloride (145 mg, 106%) as a yellow solid which was used directly in the next step without further purification. LCMS (ESI) m/z: 151.5 [M+H]+.
    • Step 5
  • Figure US20200115354A1-20200416-C00467
    • Rac-4-(((1,2-trans)-2-amino-4,4-difluorocyclohexyl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide (449 mg, 0.97 mmol) in DMF (10 mL) was added triethylamine (329 mg, 0.45 mL, 3.25 mmol) followed by addition of rac-(1,2-trans)-4,4-difluorocyclohexane-1,2-diamine dihydrochloride (145 mg, 0.65 mmol) and the mixture stirred at rt. After 16 h, the mixture was placed in a 65° C. oil bath sealed. After 2 h, the mixture was diluted with EtOAc (75 mL), washed with saturated NaHCO3(aq) (10 mL), then with 50% saturated NaCl(aq) (4×10 mL), dried (Na2SO4), filtered and concentrated in vacuo. Purification by flash column chromatography through Si gel (0-100% EtOAc/CH2Cl2) provided rac-4-(((1,2-trans)-2-amino-4,4-difluorocyclohexyl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (138 mg, 36%) as a yellow oil. LCMS (ESI) m/z: 590.9 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 7.74 (d, J=7.0 Hz, 1H), 7.38 (d, J=3.6 Hz, 1H), 7.20 (d, J=8.4 Hz, 1H), 6.96 (d, J=3.6 Hz, 1H), 6.47 (d, J=12.4 Hz, 1H), 6.42-6.22 (m, 2H), 5.19 (s, 2H), 4.84 (d, J=7.5 Hz, 1H), 3.84-3.68 (m, 6H), 3.20-2.93 (m, 2H), 2.50-2.34 (m, 1H), 2.26-2.13 (m, 1H), 2.13-2.03 (m, 1H), 1.98-1.69 (m, 2H), 1.63-1.44 (m, 3H).
    • Step 6
  • Figure US20200115354A1-20200416-C00468
    • Rac-5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1,2-trans)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of rac-4-(((1,2-trans)-2-amino-4,4-difluorocyclohexyl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (138 mg, 0.23 mmol) in MeOH (5 mL) was added 37% w/w aqueous formaldehyde (189 mg, 0.17 mL, 2.33 mmol) followed by addition of sodium cyanoborohydride (43 mg, 0.70 mmol) and the mixture was stirred at rt overnight. After 16 h, volatiles were removed in vacuo and the crude residue partitioned between EtOAc (50 mL) and saturated NaHCO3(aq) (10 mL). The phases were separated and the organic extract dried (Na2SO4), filtered and concentrated in vacuo. Purification by flash column chromatography through Si gel (0-100% EtOAc/CH2Cl2) provided rac-5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1,2-trans)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (64 mg, 440%) as a clear waxy gum. LCMS (ESI) m/z: 619.0 [M+H]+.
    • Step 7
  • Figure US20200115354A1-20200416-C00469
    • 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1R,2R)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Rac-5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1,2-trans)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (63 mg, 0.10 mmol) was separated by using chiral HPLC (Chiralpak IB (250 mm*20 mm, 5 um), MeOH:DCM:hexane 2:5:93 containing 0.1% of diethylamine, 15 ml/min) to afford 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (first peak, 21 mg, 33%) as a white solid. LCMS (ESI) m/z: 619.0 [M+H]+. Also afforded afford 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1R,2R)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (second peak, 21 mg, 33%) as a white solid. LCMS (ESI) m/z: 618.9 [M+H]+. Absolute configuration was arbitrarily assigned to each enantiomer.
    • Step 8
  • Figure US20200115354A1-20200416-C00470
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • 5-Chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (21 mg, 0.03 mmol) was treated with 95% formic acid (2 mL) and the mixture stirred at rt. After 30 min, volatiles were removed in vacuo and the crude residue purified by C18 reverse phase flash chromatography (0-100% MeCN/10 mM aqueous NH4CO2H, pH=3.8). Appropriate fractions were combined and lyophilized to provide 5-chloro-4-(((1S,2S)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate (14 mg, 805%) as a white solid. LCMS (ESI) m/z: 469.0 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 12.64 (s, 1H), 8.14 (s, 1H), 7.58 (d, J=7.3 Hz, 1H), 7.24 (d, J=2.6 Hz, 1H), 6.96-6.64 (m, 2H), 5.87 (s, 1H), 3.68-3.47 (m, 1H), 2.94 (t, J=10.3 Hz, 1H), 2.16 (s, 7H), 2.12-1.95 (m, 3H), 1.94-1.73 (m, 1H), 1.44-1.27 (m, 1H).
    • Example 188
  • Figure US20200115354A1-20200416-C00471
    • 5-chloro-4-(((1R,2R)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 187 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1R,2R)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (21 mg, 0.03 mmol), 5-chloro-4-(((1R,2R)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate was obtained (14 mg, 80%) as a white solid. LCMS (ESI) m/z: 469.0 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 12.64 (s, 1H), 8.14 (s, 1H), 7.58 (d, J=7.3 Hz, 1H), 7.23 (d, J=4.4 Hz, 1H), 6.93-6.67 (m, 2H), 5.87 (d, J=5.7 Hz, 1H), 3.67-3.46 (m, 1H), 2.94 (t, J=10.0 Hz, 1H), 2.16 (s, 7H), 2.09-1.95 (m, 3H), 1.94-1.74 (m, 1H), 1.46-1.27 (m, 1H).
    • Example 189
  • Figure US20200115354A1-20200416-C00472
    • 4-((trans)-2-(ethyl(methyl)amino)cyclohexyl)-6-fluoro-N-(thiazol-2-yl)-3,4-dihydro-2H-benzo[b][1,4]oxazine-7-sulfonamide
  • Starting with racemic mixture of (trans)-N1-ethyl-N1-methylcyclohexane-1,2-diamine and following the procedures described in Example 54 and making non-critical variations the title compound was obtained as a colorless solid and a mixture of enantiomers. LCMS (ESI) m/z: 455.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.96 (s, 1H), 7.25 (d, J=4.6 Hz, 1H), 7.17 (dd, J=13.3, 9.5 Hz, 1H), 7.06 (dd, J=7.3, 3.9 Hz, 1H), 6.82 (d, J=4.6 Hz, 1H), 6.51 (s, 2H), 4.39 (dd, J=10.8, 5.6 Hz, 1H), 4.21 (dt, J=10.8, 6.2 Hz, 1H), 4.04 (dt, J=10.8, 3.8 Hz, 1H), 3.64 (t, J=12.0 Hz, 1H), 3.26 (s, 3H), 3.07-3.01 (m, 1H), 2.67 (d, J=4.8 Hz. 3H), 2.23-1.99 (m, 1H), 1.84-1.31 (mn, 8H), 1.23 (t, J=7.1 Hz, 3H), 1.16 (t, J=7.1 Hz, 1H).
    • Example 190
  • Figure US20200115354A1-20200416-C00473
    • 5-Chloro-2-fluoro-4-(((1S,2S)-2-((1-methylazetidin-3-yl)(2,2,2-trifluoroethyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00474
    • Step 1
  • Figure US20200115354A1-20200416-C00475
    • tert-Butyl 3-(((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)amino)azetidine-1-carboxylate
  • 4-(((1S,2S)-2-Aminocyclohexyl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (412 mg, 0.74 mmol) and 1-Boc-3-azetidinone (228 mg, 1.34 mmol) were combined in DCM (3 mL) and to this was added sodium triacetoxyborohydride (779 mg, 3.71 mmol) and the mixture was stirred at room temperature. After 16 hours, the mixture was diluted with MeOH (5 mL) and concentrated in vacuo (repeated 3 times). The crude residue was diluted with EtOAc (50 mL) and saturated aqueous NaHCO3 solution (10 mL) and the phases were separated. The organic phase was dried (Na2SO4), filtered and concentrated. The crude product was purified by flash column chromatography through Si gel (EtOAc/DCM) to provide tert-butyl 3-(((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)amino)azetidine-1-carboxylate (498 mg, 94% yield). LCMS (ESI) m/z: 710.2 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00476
    • 5-Chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-((1-methylazetidin-3-yl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of tert-butyl 3-(((1S,2S)-2-((2-chloro-4-(N-(2,4-dimethoxybenzyl)-N-(thiazol-2-yl)sulfamoyl)-5-fluorophenyl)amino)cyclohexyl)amino)azetidine-1-carboxylate (498 mg, 0.70 mmol) in THF (3.5 mL) was added 2 M lithium aluminum hydride solution in THF (701 uL, 1.4 mmol). The mixture was stirred at 60° C. for 70 minutes. The reaction was then cooled to 0° C. and aqueous sodium sulfate decahydrate was added slowly until no more bubbling occurred then stirred at room temperature for 15 minutes. The mixture was filtered, rinsed with DCM and EtOAc and concentrated to provide 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-((1-methylazetidin-3-yl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (441 mg, 100%) which was used without further purification in the subsequent step. LCMS (ESI) m/z: 624.1 [M+H]+.
    • Step 3
  • Figure US20200115354A1-20200416-C00477
    • 5-Chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2s)-2-((1-methylazetidin-3-yl)(2,2,2-trifluoroethyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • 5-Chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-((1-methylazetidin-3-yl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (90 mg, 0.14 mmol) was dissolved in DMF (0.7 mL) and THF (0.7 mL). To this solution was added DIPEA (100 uL, 0.58 mol) followed by 2,2,2-trifluoroethyl trifluoromethanesulfonate (47 mg, 0.2 mmol). The reaction was stirred at room temperature for 18 hours then poured into EtOAc (50 mL) and washed with saturated aqueous brine solution (3×20 mL). The organic layer was dried over Na2SO4 and concentrated. The crude product was purified by flash column chromatography through Si gel (EtOAc/DCM) to provide 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-((1-methylazetidin-3-yl)(2,2,2-trifluoroethyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (39 mg, 38%). LCMS (ESI) m/z: 706.2 [M+H]+.
    • Step 4
  • Figure US20200115354A1-20200416-C00478
    • 5-Chloro-2-fluoro-4-(((1S,2S)-2-((1-methylazetidin-3-yl)(2,2,2-trifluoroethyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 115 and making non-critical variations to substitute 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2S)-2-((2-hydroxy-2-methylpropyl)(methyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-4-(((1S,2s)-2-((1-methylazetidin-3-yl)(2,2,2-trifluoroethyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (39 mg, 0.05 mmol), 5-chloro-2-fluoro-4-(((1S,2S)-2-((1-methylazetidin-3-yl)(2,2,2-trifluoroethyl)amino)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide (14 mg, 53%) was obtained. LCMS (ESI) m/z: 556.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.77 (d, J=7.0 Hz, 1H), 7.35 (d, J=10.8 Hz, 1H), 7.19 (d, J=3.9 Hz, 1H), 6.75 (d, J=4.1 Hz, 1H), 3.27-3.06 (m, 5H), 2.80-2.57 (m, 2H), 2.38 (s, 3H), 2.29-2.15 (m, 1H), 1.85-1.72 (m, 1H), 1.72-1.46 (m, 3H), 1.42-1.19 (m, 3H), 0.93-0.72 (m, 1H).
    • Example
  • Figure US20200115354A1-20200416-C00479
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)-5-fluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Figure US20200115354A1-20200416-C00480
    • Step 1
  • Figure US20200115354A1-20200416-C00481
    • 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)-5-fluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-fluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4R)-2-(dimethylamino)-4-fluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Iron(III) oxalate hexahydrate (416 mg, 0.86 mmol) was suspended in H2O (20 mL) and placed in a 90° C. oil bath for 10 min to provide a homogeneous yellow solution. This solution was then cooled to 0° C. and sparged with N2 for 5 min before addition of a solution of Selectfluor (305 mg, 0.86 mmol) in MeCN (10 mL) followed by addition of a solution of 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,6S)-6-(dimethylamino)cyclohex-3-en-1-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide prepared in Example 141 (250 mg, 0.43 mmol) in MeCN (10 mL). Sodium Borohydride (50 mg, 1.38 mmol) was then added and after 2 min a further portion of Sodium Borohydride (50 mg, 1.38 mmol) was added and the mixture stirred at rt. After 1 h, the mixture was diluted with concentrated aqueous NH4OH (10 mL) and organics extracted with a solution of 10% MeOH in CH2Cl2 (2×50 mL). The organic extracts were combined, dried (Na2SO4), filtered and concentrated in vacuo. Purification by flash column chromatography through Si gel (0-100% EtOAc/CH2Cl2) provided 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)-5-fluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (16 mg, 6%) as a clear waxy gum. LCMS (ESI) m/z: 601.3 [M+H]+. Also isolated was 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-fluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide and 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4R)-2-(dimethylamino)-4-fluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide as an inseparable mixture of two fluorine isomers (16 mg, 6%). LCMS (ESI) m/z: 601.2 [M+H]+. Fluorine regiochemistry and stereochemistry arbitrarily assigned to all isomers obtained.
    • Step 5
  • Figure US20200115354A1-20200416-C00482
    • 5-Chloro-4-(((1S,2S)-2-(dimethylamino)-5-fluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 187 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)-5-fluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (16 mg, 0.03 mmol), 5-chloro-4-(((1S,2S)-2-(dimethylamino)-5-fluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate was obtained (14 mg, 94%) as a white solid. LCMS (ESI) m/z: 451.0 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 8.29 (s, 1H), 8.16 (s, 1H), 7.56 (t, J=7.3 Hz, 1H), 7.17-6.90 (m, 1H), 6.80-6.41 (m, 2H), 5.87-5.55 (m, 1H), 5.04 (d, J=48.8 Hz, 1H), 3.02-2.82 (m, 1H), 2.21-2.12 (m, 6H), 2.12-2.02 (m, 1H), 2.01-1.92 (m, 1H), 1.91-1.77 (m, 1H), 1.62 (dt, J=39.7, 13.2 Hz, 1H), 1.44 (ddd, J=27.7, 13.7 Hz, 1H). [aminothiazole NH not observed and one C—H hidden under H2O peak].
    • Example 192 & Example 193
  • Figure US20200115354A1-20200416-C00483
    • 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-fluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1S,2S,4R)-2-(dimethylamino)-4-fluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • The mixture of 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-fluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide and 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4R)-2-(dimethylamino)-4-fluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (16 mg, 0.03 mmol) fluorine isomers obtained in Example 191 was treated with 95% formic acid (2 mL) and stirred at rt. After 30 min, volatiles were removed in vacuo and the crude material purified by reverse phase prep-HPLC (20-40% MeCN/10 mM aqueous NH4CO2H, pH: 3.8 gradient over 10 min, XBridge BEH C18 OBD Prep Column, 130 Å, 5 μm, 30 mm×50 mm). Appropriate fractions combined and lyophilized to provide: 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-fluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (3 mg, 25%) as a white solid, LCMS (ESI) m/z: 451.1 [M+H]+, 1H NMR (400 MHz, d6-DMSO) δ 8.28 (s, 2H), 7.56 (d, J=7.2 Hz, 1H), 6.95 (d, J=3.7 Hz, 1H), 6.47 (d, J=3.7 Hz, 1H), 6.35 (d, J=12.3 Hz, 1H), 5.59 (d, J=3.7 Hz, 1H), 4.93 (d, J=47.9 Hz, 1H), 2.75-2.63 (m, 2H), 2.16 (s, 6H), 2.09-1.96 (m, 1H), 1.76-1.67 (m, 1H), 1.65-1.35 (m, 3H); and 5-chloro-4-(((1S,2S,4R)-2-(dimethylamino)-4-fluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (7 mg, 58%) as a white solid, LCMS (ESI) m/z: 451.0 [M+H]+, 1H NMR (400 MHz, d6-DMSO) δ 8.20 (s, 1H), 7.56 (d, J=7.3 Hz, 1H), 7.03 (d, J=3.9 Hz, 1H), 6.76 (d, J=12.7 Hz, 1H), 6.56 (d, J=3.9 Hz, 1H), 5.59 (d, J=5.0 Hz, 1H), 4.73 (dtd, J=15.1, 10.5, 5.2 Hz, 1H), 2.97-2.86 (m, 1H), 2.70-2.57 (m, 1H), 2.45-2.30 (m, 2H), 2.14 (s, 6H), 1.86-1.74 (m, 1H), 1.44 (dd, J=20.7, 9.9 Hz, 2H), 1.26 (dd, J=25.2, 9.4 Hz, 1H). Fluorine regiochemistry and stereochemistry arbitrarily assigned to each isomer.
    • Example 194
  • Figure US20200115354A1-20200416-C00484
    • 5-Chloro-4-(((1S,3S,4S,6R)-7,7-dichloro-4-(dimethylamino)bicyclo 14.1.01 heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Figure US20200115354A1-20200416-C00485
    Figure US20200115354A1-20200416-C00486
    • Step 1
  • Figure US20200115354A1-20200416-C00487
    • Rac-di-tert-butyl((3,4-trans)-7,7-dichlorobicyclo[4.1.0]heptane-3,4-diyl)dicarbamate
  • To a solution of rac-di-tert-butyl(1,2-trans)-cyclohex-4-ene-1,2-diyldicarbamate (500 mg, 1.6 mmol) and tetrabutylammonium bromide (52 mg, 0.16 mmol) in CHCl3 (2 mL) was added 50% w/w aqueous NaOH (1.65 mL, 32 mmol) and the mixture stirred at rt over the weekend. After 3 days, the reaction mixture which had become a solid mass was partitioned between H2O (25 mL) and CH2Cl2 (50 mL) and the phases were separated. The organic extract was dried (Na2SO4), filtered and concentrated in vacuo. Purification by flash chromatography through Si gel (0-100% EtOAc/CH2Cl2) provided rac-di-tert-butyl((3,4-trans)-7,7-dichlorobicyclo[4.1.0]heptane-3,4-diyl)dicarbamate (155 mg, 24%) as a white solid contaminated with ˜20% rac-di-tert-butyl(1,2-trans)-cyclohex-4-ene-1,2-diyldicarbamate starting material. Used as is without further purification. LCMS (ESI) m/z: 395.1, 397.1 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00488
    • Rac-(3,4-trans)-7,7-dichlorobicyclo[4.1.0]heptane-3,4-diamine dihydrochloride
  • To a solution of rac-di-tert-butyl((3,4-trans)-7,7-dichlorobicyclo[4.1.0]heptane-3,4-diyl)dicarbamate (287 mg, 0.73 mmol) in EtOAc (5 mL) was added 4N HCl in dioxanes (2 mL, 8 mmol) and stirred at rt. After 16 h, the mixture was concentrated in vacuo to provide crude rac-(3,4-trans)-7,7-dichlorobicyclo[4.1.0]heptane-3,4-diamine dihydrochloride (210 mg, 108%) as a yellow solid which was used directly in the next step. LCMS (ESI) m/z: 195.2, 197.3 [M+H]+.
    • Step 3
  • Figure US20200115354A1-20200416-C00489
    • Rac-4-((1,3-trans), (4,6-cis), (3,4-trans))-4-amino-7,7-dichlorobicyclo[4.1.0]heptan-3-yl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & Rac-4-((1,3-cis), (4,6-trans), (3,4-trans))-4-amino-7,7-dichlorobicyclo[4.1.0]heptan-3-yl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide (536 mg, 1.16 mmol) and rac-(3,4-trans)-7,7-dichlorobicyclo[4.1.0]heptane-3,4-diamine dihydrochloride (208 mg, 0.78 mmol) in DMF (10 mL) was added triethylamine (471 mg, 0.65 mL, 4.66 mmol) and the mixture stirred at rt overnight. After 16 h, the mixture was diluted with EtOAc (50 mL), washed with saturated NaHCO3(aq) (10 mL), then with 50% saturated NaCl(aq) (4×10 mL), dried (Na2SO4), filtered and concentrated in vacuo. Purification by flash column chromatography through Si gel (0-100% EtOAc/CH2Cl2 followed by 0-20% MeOH/CH2Cl2) provided two cyclopropane diastereomers: rac-4-((1,3-trans), (4,6-cis), (3,4-trans))-4-amino-7,7-dichlorobicyclo[4.1.0]heptan-3-yl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (65 mg, 13%) as a yellow oil, LCMS (ESI) m/z: 636.7 [M+H]+; and rac-4-((1,3-cis), (4,6-trans), (3,4-trans))-4-amino-7,7-dichlorobicyclo[4.1.0]heptan-3-yl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (88 mg, 18%) as a yellow oil, LCMS (ESI) m/z: 637.1 [M+H]+. Relative cyclopropane stereochemistry arbitrarily assigned to each isomer.
    • Step 4
  • Figure US20200115354A1-20200416-C00490
    • Rac-5-chloro-4-((1,3-trans), (4,6-cis), (3,4-trans))-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & rac-5-chloro-4-((1,3-cis), (4,6-trans), (3,4-trans))-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • To a solution of rac-4-((1,3-trans), (4,6-cis), (3,4-trans))-4-amino-7,7-dichlorobicyclo[4.1.0]heptan-3-yl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (66 mg, 0.10 mmol) in MeOH (5 mL) was added 37% w/w aqueous formaldehyde (84 mg, 0.077 mL, 1.04 mmol) followed by addition of sodium cyanoborohydride (19 mg, 0.31 mmol) and the mixture was stirred at rt. After 16 h, volatiles were removed in vacuo and the crude residue partitioned between EtOAc (50 mL) and saturated NaHCO3(aq) (10 mL). The phases were separated and the organic extract dried (Na2SO4), filtered and concentrated in vacuo. Purification by flash column chromatography through Si gel (0-100% EtOAc/CH2Cl2) provided rac-5-chloro-4-((1,3-trans), (4,6-cis), (3,4-trans))-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (24 mg, 35%) as a clear oil. LCMS (ESI) m/z: 663.1, 665.4 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 7.73 (d, J=7.1 Hz, 1H), 7.39 (d, J=3.6 Hz, 1H), 7.21 (d, J=7.9 Hz, 1H), 6.97 (d, J=3.6 Hz, 1H), 6.41-6.34 (m, 2H), 6.30 (d, J=12.4 Hz, 1H), 6.09 (s, 1H), 5.20 (s, 2H), 3.75 (s, 6H), 2.93-2.66 (m, 3H), 2.22-2.12 (m, 7H), 2.00-1.83 (m, 3H), 1.36 (t, J=13.0 Hz, 1H).
  • Following the procedure described above and making non-critical variations to replace rac-4-((1,3-trans), (4,6-cis), (3,4-trans))-4-amino-7,7-dichlorobicyclo[4.1.0]heptan-3-yl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with rac-4-((1,3-cis), (4,6-trans), (3,4-trans))-4-amino-7,7-dichlorobicyclo[4.1.0]heptan-3-yl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (88 mg, 0.14 mmol), rac-5-chloro-4-((1,3-cis), (4,6-trans), (3,4-trans))-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (43 mg, 47%) was obtained as a clear oil. LCMS (ESI) m/z: 663.0, 664.9 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 7.71 (d, J=6.9 Hz, 1H), 7.39 (d, J=3.4 Hz, 1H), 7.21 (d, J=8.2 Hz, 1H), 6.96 (d, J=3.5 Hz, 1H), 6.41-6.33 (m, 2H), 6.25 (d, J=12.4 Hz, 1H), 6.12 (s, 1H), 5.19 (s, 2H), 3.75 (s, 6H), 3.21-3.10 (m, 1H), 2.77 (dd, J=14.0, 5.6 Hz, 1H), 2.51 (td, J=12.2, 4.0 Hz, 1H), 2.37 (ddd, J=14.4, 10.5, 4.1 Hz, 1H), 2.19 (s, 6H), 1.94 (td, J=10.4, 4.0 Hz, 1H), 1.84-1.70 (m, 2H), 1.63-1.47 (m, 1H).
    • Step 5
  • Figure US20200115354A1-20200416-C00491
    • 5-Chloro-4-(((1S,3S,4S,6R)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1S,3R,4R,6R)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1R,3S,4S,6S)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide & 5-chloro-4-(((1R,3R,4R,6S)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Rac-5-chloro-4-((1,3-trans), (4,6-cis), (3,4-trans))-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (23 mg, 0.03 mmol) was separated using chiral HPLC (Chiralpak IB (250 mm*20 mm, 5 um), MeOH:DCM:hexane 2:8:90 containing 0.1% of diethylamine, 15 ml/min) to afford: 5-chloro-4-(((1S,3S,4S,6R)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (peak 1, 6 mg, 26%), as a white solid, LCMS (ESI) m/z: 662.9, 664.9 [M+H]+; and 5-chloro-4-(((1S,3R,4R,6R)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (peak 2, 7 mg, 30%) as a white solid, LCMS (ESI) m/z: 663.0, 664.8 [M+H]+.
  • In addition, rac-5-chloro-4-((1,3-cis), (4,6-trans), (3,4-trans))-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (43 mg, 0.06 mmol) was separated using chiral HPLC (Chiralpak IB (250 mm*20 mm, 5 um), MeOH:DCM:hexane 2:8:90 containing 0.1% of diethylamine, 15 ml/min) to afford: 5-chloro-4-(((1R,3S,4S,6S)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (peak 1, 11 mg, 26%), as a white solid, LCMS (ESI) m/z: 663.0, 665.0 [M+H]; and 5-chloro-4-(((1R,3R,4R,6S)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (peak 2, 13 mg, 30%) as a white solid, LCMS (ESI) m/z: 662.9, 664.9 [M+H]+. Cyclopropane relative stereochemistry and absolute stereochemistry arbitrarily assigned.
    • Step 6
  • Figure US20200115354A1-20200416-C00492
    • 5-Chloro-4-(((1S,3S,4S,6R)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 187 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-4-(((1S,3S,4S,6R)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (6 mg, 0.01 mmol), 5-chloro-4-(((1S,3S,4S,6R)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate was obtained (4 mg, 79%) as a white solid. LCMS (ESI) m/z: 512.9, 514.9 [M+H]+. 1H NMR (400 MHz, d6-DMSO) [thiazole N—H signal not observed] δ 8.18 (s, 1H), 7.57 (d, J=6.9 Hz, 1H), 7.07 (d, J=3.1 Hz, 1H), 6.77 (d, J=12.3 Hz, 1H), 6.61 (d, J=3.4 Hz, 1H), 5.80 (s, 1H), 3.12-3.01 (m, 1H), 2.78-2.61 (m, 2H), 2.10 (s, 6H), 2.01-1.92 (m, 3H), 1.33-1.03 (m, 2H). Cyclopropane relative stereochemistry and absolute stereochemistry arbitrarily assigned.
    • Example 195
  • Figure US20200115354A1-20200416-C00493
    • 5-Chloro-4-(((1S,3R,4R,6R)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 187 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-4-(((1S,3R,4R,6R)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (7 mg, 0.01 mmol), 5-chloro-4-(((1S,3R,4R,6R)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate was obtained (4 mg, 68%) as a white solid. Absolute stereochemistry and relative cyclopropane stereochemistry was arbitrarily assigned LCMS (ESI) m/z: 512.9 [M+H]+. 1H NMR (400 MHz, d6-DMSO) [thiazole N—H signal not observed] δ 8.16 (s, 1H), 7.57 (d, J=7.2 Hz, 1H), 7.09 (d, J=3.8 Hz, 1H), 6.78 (d, J=12.6 Hz, 1H), 6.63 (d, J=3.7 Hz, 1H), 6.56 (s, 1H), 5.82 (s, 1H), 3.14-3.03 (m, 1H), 2.77-2.62 (m, 2H), 2.10 (s, 6H), 2.01-1.91 (m, 3H), 1.30-1.06 (m, 2H).
    • Example 196
  • Figure US20200115354A1-20200416-C00494
    • 5-Chloro-4-(((1R,3S,4S,6S)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 187 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-4-(((1R,3S,4S,6S)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (11 mg, 0.02 mmol), 5-chloro-4-(((1R,3S,4S,6S)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate was obtained (7 mg, 75%) as a white solid. Absolute stereochemistry and relative cyclopropane stereochemistry was arbitrarily assigned. LCMS (ESI) m/z: 513.0, 515.0 [M+H]+. 1H NMR (400 MHz, d6-DMSO) [thiazole N—H signal not observed] δ 8.14 (s, 1H), 7.59 (d, J=7.1 Hz, 1H), 7.18 (d, J=3.7 Hz, 1H), 6.72 (d, J=3.6 Hz, 1H), 6.54 (s, 1H), 6.39 (d, J=12.4 Hz, 1H), 5.95 (s, 1H), 3.23-3.17 (m, 1H), 2.13 (s, 6H), 2.08-1.98 (m, 2H), 1.93-1.77 (m, 3H), 1.43-1.17 (m, 2H).
    • Example 197
  • Figure US20200115354A1-20200416-C00495
    • 5-Chloro-4-(((1R,3R,4R,6S)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 187 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-4-(((1R,3R,4R,6S)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide (13 mg, 0.02 mmol), 5-chloro-4-(((1R,3R,4R,6S)-7,7-dichloro-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide formate was obtained (8 mg, 73%) as a white solid. Absolute stereochemistry and relative cyclopropane stereochemistry was arbitrarily assigned. LCMS (ESI) m/z: 513.0, 515.0 [M+H]+. 1H NMR (400 MHz, d6-DMSO) [thiazole N—H signal not observed] δ 8.15 (s, 1H), 7.58 (d, J=7.2 Hz, 1H), 7.14 (d, J=4.2 Hz, 1H), 6.68 (d, J=4.2 Hz, 1H), 6.55 (s, 1H), 6.37 (d, J=12.4 Hz, 1H), 5.92 (d, J=3.7 Hz, 1H), 3.22-3.17 (m, 1H), 2.17-2.08 (m, 7H), 2.08-1.91 (m, 2H), 1.92-1.77 (m, 2H), 1.45-1.06 (m, 2H).
    • Example 198
  • Figure US20200115354A1-20200416-C00496
    • N-((1S,2S)-2-((2-Chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)-cyclohexyl)-2-(dimethylamino)-N,2-dimethylpropanamide
  • Following the procedure described in Example 179 and making non-critical variations as required to replace 1-methylazetidine-3-carboxylic acid with 2-(dimethylamino)-2-methyl-propanoic acid, N-((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)-cyclohexyl)-2-(dimethylamino)-N,2-dimethylpropanamide was obtained. LCMS (ESI) m/z: 532.1 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 7.50 (d, J=7.3 Hz, 1H), 7.24 (d, J=4.5 Hz, 1H), 6.79 (d, J=4.5 Hz, 2H), 5.67 (s, 1H), 4.51 (s, 1H), 3.66 (s, 1H), 3.17 (s, 3H), 1.96 (s, 8H), 1.66 (d, J=55.5 Hz, 4H), 1.31 (s, 4H), 1.10 (s, 3H), 0.99 (s, 3H).
    • Example 199
  • Figure US20200115354A1-20200416-C00497
    • (S)—N-((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)-N,1-dimethylpyrrolidine-3-carboxamide
  • Following the procedure described in Example 179 and making non-critical variations as required to replace 1-methylazetidine-3-carboxylic acid with (3S)-1-methylpyrrolidine-3-carboxylic acid, (S)—N-((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)-N,1-dimethylpyrrolidine-3-carboxamide was obtained. LCMS (ESI) m/z: 530.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.20 (s, 1H), 7.52 (t, J=6.5 Hz, 1H), 7.15 (dd, J=6.5, 4.4 Hz, 1H), 6.88-6.63 (m, 2H), 5.69-5.29 (m, 1H), 4.45 (s, 1H), 3.87-3.53 (m, 2H), 3.32-3.01 (m, 1H), 3.01-2.79 (m, 2H), 2.77 (s, 2H), 2.60 (s, 1H), 2.45 (s, J=16.8 Hz, 1H), 2.37 (s, J=12.2 Hz, 2H), 2.18-1.80 (m, 3H), 1.80-1.47 (m, 5H), 1.45-1.19 (m, 3H).
    • Example 200
  • Figure US20200115354A1-20200416-C00498
    • (R)—N-((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)-N,1-dimethylpyrrolidine-3-carboxamide
  • Following the procedure described in Example 179 and making non-critical variations as required to replace 1-methylazetidine-3-carboxylic acid with (3R)-1-methylpyrrolidine-3-carboxylic acid, (R)—N-((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)-N,1-dimethylpyrrolidine-3-carboxamide was obtained. LCMS (ESI) m/z: 530.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.20 (s, 1H), 7.52 (dd, J=7.3, 5.0 Hz, 1H), 7.15 (dd, J=7.0, 4.3 Hz, 1H), 6.87-6.64 (m, 2H), 5.71-5.26 (m, 1H), 4.45 (s, 1H), 3.89-3.52 (m, 2H), 3.27 (bs, 1H), 2.97 (dt, J=34.8, 12.2 Hz, 3H), 2.77 (s, J=16.7 Hz, 3H), 2.72-2.61 (m, 1H), 2.59 (s, 1H), 2.54 (s, 1H), 2.23-1.81 (m, 3H), 1.81-1.44 (m, 6H), 1.32 (d, J=8.8 Hz, 3H).
    • Example 201
  • Figure US20200115354A1-20200416-C00499
    • (S)—N-((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)-N,1-dimethylpyrrolidine-2-carboxamide
  • Following the procedure described in Example 179 and making non-critical variations as required to replace 1-methylazetidine-3-carboxylic acid with (2S)-1-methylpyrrolidine-2-carboxylic acid, (S)—N-((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)-cyclohexyl)-N,1-dimethylpyrrolidine-2-carboxamide was obtained. LCMS (ESI) m/z: 530.3 [M+H]+. 1H NMR (400 MHz, d6-DMSO) [˜1:1 mixture of rotomers by NMR] δ 11.18 (br, s, 1H), 7.55 (d, J=7.3 Hz, 1H), 7.26 (d, J=4.6 Hz, 1H), 6.96-6.83 (m, 1H), 6.81 (d, J=4.6 Hz, 1H), 5.98 (d, J=9.7 Hz, 0.5H), 5.49 (s, 0.5H), 4.90 (t, J=8.4 Hz, 0.5H), 4.47 (s, 0.5H), 4.28 (t, J=6.7 Hz, 0.5H), 3.88 (dd, J=10.2, 3.7 Hz, 0.5H), 3.75 (s, 0.5H), 3.55 (dd, J=9.3, 5.9 Hz, 1H), 3.51-3.44 (m, 0.5H), 3.02 (dd, J=19.7, 8.7 Hz, 0.5H), 2.98-2.90 (m, 0.5H), 2.76 (s, 1.5H), 2.72 (s, 1.5H), 2.65 (s, 3H), 2.44-2.34 (m, J=12.3, 6.2 Hz, 0.5H), 2.24 (td, J=17.4, 8.8 Hz, 0.5H), 2.16-2.04 (m, J=8.3, 4.2 Hz, 0.5H), 2.03-1.86 (m, J=9.0, 5.8 Hz, 2H), 1.86-1.73 (m, 2H), 1.73-1.62 (m, 3H), 1.53-1.18 (m, 3.5H).
    • Example 202
  • Figure US20200115354A1-20200416-C00500
    • (S)—N-((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)-N,1-dimethylazetidine-2-carboxamide
  • Following the procedure described in Example 179 and making non-critical variations as required to replace 1-methylazetidine-3-carboxylic acid with (2S)-1-methylazetidine-2-carboxylic acid, (S)—N-((1S,2S)-2-((2-chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)-cyclohexyl)-N,1-dimethylazetidine-2-carboxamide was obtained. LCMS (ESI) m/z: 516.2 [M+H]+. 1H NMR (400 MHz, d6-DMSO) [˜1:1 mixture of rotomers by NMR] δ 7.54 (t, J=7.4 Hz, 1H), 7.18 (dd, J=8.0, 4.4 Hz, 1H), 6.86 (d, J=13.3 Hz, 0.5H), 6.83-6.66 (m, 1.5H), 5.93 (d, J=9.9 Hz, 0.5H), 5.36 (s, 0.5H), 4.94 (s, 0.5H), 4.44 (s, 1H), 3.85-3.43 (m, 3.5H), 2.61 (s, 1.5H), 2.60 (s, 1.5H), 2.51 (s, 1.5H), 2.45 (s, 1.5H), 2.25 (dd, J=18.5, 9.7 Hz, 1H), 1.98 (d, J=8.0 Hz, 0.5H), 1.89 (d, J=13.0 Hz, 0.5H), 1.74 (d, J=10.3 Hz, 1H), 1.69-1.52 (m, 4H), 1.52-1.18 (m, 4H).
    • Example 203
  • Figure US20200115354A1-20200416-C00501
    • 5-cyclobutyl-4-(((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 91 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide, 5-cyclobutyl-4-(((1S,2S,4S)-2-(dimethylamino)-4-(trifluoromethyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 516.3 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.55 (s, 1H), 8.30 (d, J=6.0 Hz, 1H), 7.47 (d, J=8.0 Hz, 1H), 7.00 (d, J=6.8 Hz, 1H), 6.48 (d, J=14.4 Hz, 1H), 5.21 (s, 1H), 3.39-3.37 (m, 1H), 3.28-3.23 (m, 1H), 2.73-2.67 (m, 1H), 2.60-2.55 (m, 1H), 2.45-2.40 (m, 2H), 2.37-2.31 (m, 2H), 2.19 (s, 3H), 2.19 (s, 3H), 2.04-2.02 (m, 2H), 1.89-1.80 (m, 3H), 1.57-1.51 (m, 1H), 1.36-1.27 (m, 1H), 1.20-1.19 (m, 1H).
    • Example 204
  • Figure US20200115354A1-20200416-C00502
    • (R)—N-((1S,2S)-2-((2-Chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)-N,1-dimethylpyrrolidine-2-carboxamide
  • Following the procedure described in Example 179 and making non-critical variations as required to replace 1-methylazetidine-3-carboxylic acid with (2R)-1-methylpyrrolidine-2-carboxylic acid, (R)—N-((1S,2S)-2-((2-Chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)-N,1-dimethylpyrrolidine-2-carboxamide was obtained. LCMS (ESI) m/z: 530.1 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 7.79 (d, J=7.3 Hz, 1H), 7.14 (d, J=4.6 Hz, 1H), 6.49 (d, J=4.6 Hz, 1H), 6.26 (d, J=12.5 Hz, 1H), 5.37 (br s, 1H), 4.68 (br s, 1H), 3.34-3.19 (m, 3H), 2.88 (s, 3H), 2.45-2.36 (m, 1H), 2.33 (s, 3H), 2.26-2.08 (m, 2H), 2.04-1.89 (m, 1H), 1.88-1.72 (m, 5H), 1.65-1.37 (m, 2H), 1.36-1.20 (m, 2H).
    • Example 205
  • Figure US20200115354A1-20200416-C00503
    • (R)—N-((1S,2S)-2-((2-Chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)cyclohexyl)-N,1-dimethylazetidine-2-carboxamide
  • Following the procedure described in Example 179 and making non-critical variations as required to replace 1-methylazetidine-3-carboxylic acid with (2R)-1-methylazetidine-2-carboxylic acid, (R)—N-((1S,2S)-2-((2-Chloro-5-fluoro-4-(N-(thiazol-2-yl)sulfamoyl)phenyl)amino)-cyclohexyl)-N,1-dimethylazetidine-2-carboxamide was obtained. LCMS (ESI) m/z: 516.1 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 7.79 (d, J=7.3 Hz, 1H), 7.15 (d, J=4.6 Hz, 1H), 6.50 (d, J=4.6 Hz, 1H), 6.24 (d, J=12.5 Hz, 1H), 5.37 (br s, 1H), 4.63 (br s, 1H), 3.70 (t, J=8.3 Hz, 1H), 3.55-3.46 (m, 1H), 3.21 (br s, 1H), 2.99-2.89 (m, 1H), 2.67 (s, 3H), 2.39 (s, 3H), 2.34-2.11 (m, 3H), 1.91-1.73 (m, 3H), 1.68-1.37 (m, 2H), 1.37-1.19 (m, 2H).
    • Example 206
  • Figure US20200115354A1-20200416-C00504
    • 5-chloro-2-fluoro-4-(((1S,2S,4S)-2-(piperidin-1-yl)-4-(trifluoromethyl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide Step 1
  • Figure US20200115354A1-20200416-C00505
    • tert-butyl((1S,6S)-6-(piperidin-1-yl)-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate
  • To a solution of tert-butyl((1S,6S)-6-amino-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate (100 mg, 0.36 mmol) in MeCN (2 mL) was added 1,5-dibromopentane (82 mg, 0.36 mmol) and N,N-diisopropylethylamine (0.12 mL, 0.71 mmol). The reaction mixture was irradiated in a microwave at 90° C. for 3 h. After cooling to room temperature, the mixture was concentrated in vacuo. DCM (30 mL) was added and washed with water (20 mL×2), brine (20 mL). The organic layer was dried over anhydrous Na2SO4, filtered and concentrated in vacuo. The crude residue was purified by silica gel chromatography (solvent gradient: 0-10% EtOAc in petroleum ether) to give the title compound (42 mg, 34%) as a white solid. 1H NMR (400 MHz, CDCl3) δ 6.21 (s, 1H), 5.67 (s, 1H), 3.53-3.38 (m, 1H), 3.17-3.06 (m, 1H), 2.70-2.53 (m, 3H), 2.40-2.28 (m, 3H), 2.26-2.15 (m, 1H), 2.03-1.93 (m, 1H), 1.73-1.38 (m, 6H), 1.46 (s, 9H).
    • Step 2
  • Figure US20200115354A1-20200416-C00506
    • 5-chloro-2-fluoro-4-(((1S,2S,4S)-2-(piperidin-1-yl)-4-(trifluoromethyl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 112 and making non-critical variations as required to replace tert-butyl((1S,6S)-6-(dimethylamino)-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate with tert-butyl((1S,6S)-6-(piperidin-1-yl)-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate, 5-chloro-2-fluoro-4-(((1S,2S,4S)-2-(piperidin-1-yl)-4-(trifluoromethyl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 536.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.58 (s, 1H), 8.30 (d, J=4.4 Hz, 1H), 7.73 (d, J=7.6 Hz, 1H), 6.96 (d, J=6.4 Hz, 1H), 6.80 (d, J=13.2 Hz, 1H), 5.99 (d, J=4.4 Hz, 1H), 3.47-3.36 (m, 1H), 2.78-2.69 (m, 1H), 2.52-2.51 (m, 2H), 2.43-2.34 (m, 2H), 2.22-2.14 (m, 1H), 2.05-1.95 (m, 1H), 1.83-1.75 (m, 1H), 1.54-1.44 (m, 1H), 1.42-1.20 (m, 9H).
    • Example 207
  • Figure US20200115354A1-20200416-C00507
    • 5-Chloro-4-(((1S,3S,4S,6R)-4-(dimethylamino)-7,7-difluorobicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Step 1
  • Figure US20200115354A1-20200416-C00508
    • Rac-(3,4-trans)-Diethyl 7,7-difluorobicyclo[4.1.0]heptane-3,4-dicarboxylate
  • In a test tube was added (1S,2S)-diethyl cyclohex-4-ene-1,2-dicarboxylate (1.5 g, 6.63 mmol), methyl benzoate (825 uL, 6.63 mmol) and then sodium fluoride (10 mg, 0.23 mmol). The vial was heated at 110° C. and trimethylsilyl 2,2-difluoro-2-(fluorosulfonyl)acetate (3.9 mL, 19.89 mmol) was added over 45 minutes using a syringe pump. The mixture was cooled to room temperature, EtOAc (25 mL) and saturated sodium bicarbonate solution (10 mL) were added and the phases were separated. The organic phase was washed with brine (10 ml), dried over sodium sulfate, filtered and concentrated. The crude product was purified by flash column chromatography through Si gel (EtOAc/Hexanes) to provide rac-(3,4-trans)-diethyl 7,7-difluorobicyclo[4.1.0]heptane-3,4-dicarboxylate (Ig, 55%) as a colorless oil. LCMS (ESI) m/z: 276.9 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 4.19-4.03 (m, 4H), 2.59 (tdd, J=11.4, 5.4, 2.2 Hz, 1H), 2.44 (tdd, J=11.0, 5.0, 1.9 Hz, 1H), 2.34 (ddd, J=14.4, 9.3, 4.4 Hz, 1H), 2.28-2.18 (m, 1H), 1.88-1.75 (m, 1H), 1.75-1.55 (m, 3H), 1.28-1.19 (m, 6H).
    • Step 2
  • Figure US20200115354A1-20200416-C00509
    • Rac-(3,4-trans)-7,7-difluorobicyclo[4.1.0]heptane-3,4-dicarboxylic Acid
  • In a flask containing rac-(3,4-trans)-diethyl 7,7-difluorobicyclo[4.1.0]heptane-3,4-dicarboxylate was added lithium hydroxide monohydrate (1.0 g, 24 mmol), water (5 mL), 1,4-Dioxane (3 mL) and Methanol (0.5 mL). The mixture stirred at 25° C. for 72 hours then 6M HCl (10 mL) and EtOAc (50 mL) were added. The phases were separated and the aqueous phase was extracted with EtOAc (50 mL). The combined organic extracts were dried over sodium sulfate, filtered and concentrated to afford crude rac-((3,4-trans)-7,7-difluorobicyclo[4.1.0]heptane-3,4-dicarboxylic acid (0.76 g, 95%) as an off-white solid which was used directly in the next step without further purification. LCMS (ESI) m/z: 219.0 [M−H]−. 1H NMR (400 MHz, CDCl3) δ 2.52-2.33 (m, 3H), 2.28-2.18 (m, 1H), 1.89-1.68 (m, 3H), 1.61-1.46 (m, 1H).
    • Step 3
  • Figure US20200115354A1-20200416-C00510
    • Rac-(3,4-trans)-7,7-difluorobicyclo[4.1.0]heptane-3,4-diamine Dihydrochloride
  • In a flask containing rac-((3,4-trans)-7,7-difluorobicyclo[4.1.0]heptane-3,4-dicarboxylic acid (750 mg, 3.4 mmol) was added DMF (10 uL, 0.13 mmol) followed by oxalyl chloride (4 mL, 46.6 mmol). The suspension was stirred at room temperature for 30 minutes. The mixture was then concentrated to dryness, dissolved in 1,4-Dioxane (5 mL) and trimethylsilyl azide (1.3 mL, 9.8 mmol) was added. After 30 minutes, the mixture was heated to 90° C. for 1 hour. Concentrated HCl (1 mL, 12 mmol) was added and the oil bath was cooled to 45° C. and the mixture was stirred for 16 h. The mixture was concentrated to dryness, solubilized in 1,4-dioxane and after stirring for 2 h a precipitate appeared. The solids were filtered and washed with diethyl ether, and dried under vacuum to afford rac-(3,4-trans)-7,7-difluorobicyclo[4.1.0]heptane-3,4-diamine dihydrochloride (170 mg, 21%) as a white solid. LCMS (ESI) m/z: 162.8 [M+H]+. 1H NMR (400 MHz, methanol-d4) δ 3.51-3.34 (m, 2H), 2.49 (ddd, J=17.8, 8.6, 4.7 Hz, 1H), 2.26-2.06 (m, 2H), 2.03-1.83 (m, 2H), 1.67-1.52 (m, 1H).
    • Step 4
  • Figure US20200115354A1-20200416-C00511
    • 5-Chloro-4-(((1S,3S,4S,6R)-4-(dimethylamino)-7,7-difluorobicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 194 and making non-critical variations as required to replace rac-(3,4-trans)-7,7-dichlorobicyclo[4.1.0]heptane-3,4-diamine with rac-(3,4-trans)-7,7-difluorobicyclo[4.1.0]heptane-3,4-diamine dihydrochloride the title compound was obtained as a white solid. Absolute stereochemistry and relative cyclopropane stereochemistry was arbitrarily assigned. LCMS (ESI) m/z: 481.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.43 (br s, 1H), 7.60 (d, J=7.3 Hz, 1H), 7.23 (d, J=4.5 Hz, 1H), 6.79 (d, J=4.5 Hz, 1H), 6.49 (d, J=12.6 Hz, 1H), 6.09 (d, J=3.7 Hz, 1H), 3.08 (s, 1H), 2.73-2.62 (m, J=11.9 Hz, 1H), 2.46 (d, J=10.9 Hz, 1H), 2.23-2.10 (m, 7H), 1.89 (ddd, J=14.5, 12.9, 4.2 Hz, 1H), 1.83-1.63 (m, 2H), 1.40 (td, J=13.3, 4.1 Hz, 1H).
    • Example 208
  • Figure US20200115354A1-20200416-C00512
    • 5-Chloro-4-(((1R,3R,4R,6S)-4-(dimethylamino)-7,7-difluorobicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 194 and making non-critical variations as required to replace rac-(3,4-trans)-7,7-dichlorobicyclo[4.1.0]heptane-3,4-diamine with rac-(3,4-trans)-7,7-difluorobicyclo[4.1.0]heptane-3,4-diamine dihydrochloride the title compound was obtained as a white solid. Absolute stereochemistry and relative cyclopropane stereochemistry was arbitrarily assigned. LCMS (ESI) m/z: 481.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.60 (d, J=7.3 Hz, 1H), 7.21 (d, J=4.4 Hz, 1H), 6.76 (d, J=4.4 Hz, 1H), 6.47 (d, J=12.5 Hz, 1H), 6.07 (d, J=3.5 Hz, 1H), 3.07 (s, 1H), 2.67 (td, J=11.0, 3.7 Hz, 1H), 2.48-2.41 (m, 1H), 2.18 (dd, J=8.5, 5.1 Hz, 1H), 2.13 (s, 6H), 1.95-1.83 (m, 1H), 1.82-1.63 (m, 2H), 1.40 (td, J=13.4, 4.0 Hz, 1H).
    • Example 209
  • Figure US20200115354A1-20200416-C00513
    • 5-Chloro-4-(((1R,3S,4S,6S)-4-(dimethylamino)-7,7-difluorobicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 194 and making non-critical variations as required to replace rac-(3,4-trans)-7,7-dichlorobicyclo[4.1.0]heptane-3,4-diamine with rac-(3,4-trans)-7,7-difluorobicyclo[4.1.0]heptane-3,4-diamine dihydrochloride the title compound was obtained as a white solid. Absolute stereochemistry and relative cyclopropane stereochemistry was arbitrarily assigned. LCMS (ESI) m/z: 481.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.56 (d, J=7.3 Hz, 1H), 7.19 (d, J=4.5 Hz, 1H), 6.81 (d, J=12.8 Hz, 1H), 6.74 (d, J=4.5 Hz, 1H), 5.98 (d, J=3.8 Hz, 1H), 3.16-3.09 (m, 2H), 2.66-2.55 (m, 1H), 2.11 (s, 6H), 1.94 (dd, J=12.4, 4.1 Hz, 1H), 1.90-1.74 (m, 3H), 1.18 (td, J=12.7, 3.6 Hz, 1H).
    • Example 210
  • Figure US20200115354A1-20200416-C00514
    • 5-Chloro-4-(((1S,3R,4R,6R)-4-(dimethylamino)-7,7-difluorobicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 194 and making non-critical variations as required to replace rac-(3,4-trans)-7,7-dichlorobicyclo[4.1.0]heptane-3,4-diamine with rac-(3,4-trans)-7,7-difluorobicyclo[4.1.0]heptane-3,4-diamine dihydrochloride the title compound was obtained as a white solid. Absolute stereochemistry and relative cyclopropane stereochemistry was arbitrarily assigned. LCMS (ESI) m/z: 481.0 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.59 (d, J=7.3 Hz, 1H), 7.23 (d, J=4.5 Hz, 1H), 6.84 (d, J=12.9 Hz, 1H), 6.78 (d, J=4.5 Hz, 1H), 6.02 (d, J=3.9 Hz, 1H), 3.21-3.12 (m, 2H), 2.68-2.58 (m, 1H), 2.14 (s, 6H), 2.01-1.92 (m, 1H), 1.92-1.76 (m, 3H), 1.24 (td, J=12.1, 3.9 Hz, 1H).
    • Example 211
  • Figure US20200115354A1-20200416-C00515
    • 5-Chloro-4-(((1S,2S)-2-(dimethylamino)cyclohexy)amino)-2-fluoro-N-(5-fluoro-4-methylthiazol-2-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 172 and making non-critical variations as required to replace 5-chloro-4-methyl-thiazol-2-amine with 5-fluoro-4-methylthiazol-2-amine the title compound was obtained as a white solid. LCMS (ESI) m/z: 465.1, 467.0 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 7.51 (d, J=7.3 Hz, 1H), 6.67 (d, J=12.6 Hz, 1H), 5.65 (d, J=5.4 Hz, 1H), 2.75 (s, 1H), 2.27 (s, J=26.3 Hz, 7H), 2.14-1.82 (m, 5H), 1.69 (dd, J=65.9, 11.6 Hz, 2H), 1.42-1.06 (m, 4H).
    • Example 212
  • Figure US20200115354A1-20200416-C00516
    • 5-Chloro-2-fluoro-4-(((1S,2S)-2-((R)-3-fluoro-3-(hydroxymethyl)piperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the synthetic sequence described in Example 70 and making non-critical variations to substitute piperidine with rac-(3-fluoro-3-piperidyl)methanol, the title compound was obtained as a mixture of diastereomers. Separation of the isomers by HPLC gave 5-Chloro-2-fluoro-4-(((1S,2S)-2-((R)-3-fluoro-3-(hydroxymethyl)piperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide as a white solid. The stereochemistry of the hydroxymethyl group was arbitrarily assigned. LCMS (ESI) m/z: 520.9 [M+H]+. 1H NMR (400 MHz, d6-dmso) δ 12.75 (s, 1H), 7.59 (d, J=7.3 Hz, 1H), 7.25 (d, J=4.6 Hz, 1H), 6.80 (d, J=4.6 Hz, 1H), 6.67 (d, J=12.8 Hz, 1H), 5.94 (s, 1H), 4.80-4.71 (m, 1H), 3.47 (ddd, J=23.7, 12.2 Hz, 4.2 Hz, 1H), 3.29-3.14 (m, 2H), 2.69-2.41 (m, 4H), 2.36-2.23 (m, 1H), 2.23-2.10 (m, 1H), 1.86-1.71 (m, 2H), 1.68-1.55 (m, 2H), 1.52-1.34 (m, 4H), 1.28-1.02 (m, 3H).
    • Example 213
  • Figure US20200115354A1-20200416-C00517
    • 5-Chloro-2-fluoro-4-(((1S,2S)-2-((S)-3-fluoro-3-(hydroxymethyl)piperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide
  • Following the synthetic sequence described in Example 70 and making non-critical variations to substitute piperidine with rac-(3-fluoro-3-piperidyl)methanol, the title compound was obtained as a mixture of diastereomers. Separation of the isomers by HPLC gave 5-Chloro-2-fluoro-4-(((1S,2S)-2-((S)-3-fluoro-3-(hydroxymethyl)piperidin-1-yl)cyclohexyl)amino)-N-(thiazol-2-yl)benzenesulfonamide as a white solid. The stereochemistry of the hydroxymethyl group was arbitrarily assigned. LCMS (ESI) m/z: 520.9 [M+H]+. 1H NMR (400 MHz, d6-dmso) δ 12.75 (s, 1H), 7.58 (d, J=7.3 Hz, 1H), 7.26 (d, J=4.6 Hz, 1H), 6.82 (d, J=4.6 Hz, 1H), 6.69 (d, J=12.9 Hz, 1H), 5.95 (d, J=3.0 Hz, 1H), 4.80 (t, J=6.0 Hz, 1H), 3.47 (td, J=24.1 Hz, 5.7 Hz, 2H), 3.29-3.19 (m, 1H), 2.65-2.42 (m, 4H), 2.29-2.21 (m, 1H), 2.17 (d, 1H), 1.89-1.50 (m, 5H), 1.49-1.31 (m, 2H), 1.31-1.08 (m, 4H).
    • Example 214
  • Figure US20200115354A1-20200416-C00518
    • 5-Chloro-4-(((1S,3S,4S,6R)-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide Step 1
  • Figure US20200115354A1-20200416-C00519
    • Rac-(3S,4S)-diethyl bicyclo[4.1.0]heptane-3,4-dicarboxylate
  • In a dry flask under nitrogen was added diethylzinc solution (1M in hexane, 25.6 mL, 25.6 mmol), then DCM (100 mL) and the solution was cooled to 0° C. Trifluoroacetic acid (1.97 ml, 25.6 mmol) was added dropwise, then diiodomethane (2.06 ml, 25.6 mmol) was added. After 15 minutes, a solution of racemic diethyl(1S,2S)-diethyl cyclohex-4-ene-1,2-dicarboxylate (1.45 g, 6.41 mmol) in DCM (33 mL) was added. The mixture was warmed to room temperature slowly and after stirring for 16 h, methanol (2 mL) was added, followed by addition of saturated aqueous sodium bicarbonate (25 mL). The phases were separated. The organic phase was washed with brine (10 mL), dried over sodium sulfate, filtered and concentrated. To facilitate separation of the remaining starting alkene, it was converted to the diol via dihydroxylation. The crude product containing residual alkene starting material was diluted with DCM (10 mL), and 4-methylmorpholine N-oxide (751 mg, 6.41 mmol) and osmium tetroxide (4% wt in water, 0.5 ml, 0.080 mmol) were added and the mixture was stirred for 16 h. EtOAc (50 mL) and a solution of 50% saturated aqueous sodium thiosulfate 50% (25 mL) were added. The phases were separated and the organic phase was dried over sodium sulfate, filtered and concentrated. The crude was purified by flash column chromatography through Si gel (EtOAc/hexanes) to provide rac-(3S,4S)-diethyl bicyclo[4.1.0]heptane-3,4-dicarboxylate (1.34 g, 87%) as a colorless oil. LCMS (ESI) m/z: 241.1 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 4.18-3.99 (m, 4H), 2.54-2.37 (m, 2H), 2.36-2.28 (m, 1H), 2.20 (ddd, J=13.3, 4.5, 1.1 Hz, 1H), 1.82 (td, J=12.9, 5.1 Hz, 1H), 1.51-1.38 (m, 1H), 1.21 (td, J=7.1, 3.0 Hz, 6H), 0.97 (qdd, J=14.1, 10.2, 6.7 Hz, 2H), 0.64 (td, J=8.8, 4.9 Hz, 1H), 0.03 (q, J=5.2 Hz, 1H).
    • Step 2
  • Figure US20200115354A1-20200416-C00520
    • Rac-(3S,4S)-bicyclo[4.1.0]heptane-3,4-dicarboxylic Acid
  • In a flask containing rac-(3S,4S)-diethyl bicyclo[4.1.0]heptane-3,4-dicarboxylate (1.24 g, 5.16 mmol) was added lithium hydroxide monohydrate (1.27 g, 30.3 mmol), water (5 mL), 1,4-dioxane (3 mL) and methanol (0.5 mL). The mixture was stirred at 60° C. for 1 hour and then cooled to room temperature. 6M HCl (10 mL) and EtOAc (50 mL) were added. The phases were separated and the aqueous phase was extracted with EtOAc (50 mL). The combined organic extracts were dried over sodium sulfate, filtered and concentrated to afford crude rac-(3S,4S)-bicyclo[4.1.0]heptane-3,4-dicarboxylic acid (0.93 g, 98%) as a white solid. LCMS (ESI) m/z: 183.1 [M−H]−. 1H NMR (400 MHz, methanol-d4) δ 2.50-2.35 (m, 2H), 2.34-2.17 (m, 2H), 1.84 (td, J=12.6, 5.1 Hz, 1H), 1.45 (t, J=12.6 Hz, 1H), 1.14-0.97 (m, 2H), 0.69 (td, J=8.8, 4.9 Hz, 1H), 0.07 (q, J=5.2 Hz, 1H). 2 HO signals exchanged with the solvent and are not observed.
    • Step 3
  • Figure US20200115354A1-20200416-C00521
    • Rac-(3S,4S)-bicyclo[4.1.0]heptane-3,4-diamine Dihydrochloride
  • In a flask containing rac-((3S,4S)-bicyclo[4.1.0]heptane-3,4-dicarboxylic acid (500 mg, 2.71 mmol) was added DMF (25 uL, 0.32 mmol) followed by oxalyl chloride (5 mL, 58 mmol). The suspension was stirred at room temperature for 30 minutes. The mixture was concentrated to dryness, dissolved in 1,4-dioxane (5 mL) and trimethylsilyl azide (0.90 ml, 6.8 mmol) was added. After 30 minutes, the mixture was heated to 90° C. for 1 hour. Concentrated hydrochloric acid (2.5 ml, 30 mmol) was then added and the oil bath was cooled to 45° C. and the mixture was stirred for 16 h. The mixture was concentrated to dryness, diluted with 1,4-dioxane and after stirring for 15 minutes a precipitate appeared. The suspension was stirred for 1 hour, the solids were filtered and washed with diethyl ether, and dried under vacuum to afford rac-(3S,4S)-bicyclo[4.1.0]heptane-3,4-diamine dihydrochloride (190 mg, 35%) as an off-white solid. LCMS (ESI) m/z: 126.9 [M+H]+. 1H NMR (400 MHz, methanol-d4) δ 3.42-3.33 (m, 2H), 2.65-2.46 (m, 1H), 2.24-2.12 (m, 1H), 2.03 (dt, J=8.9, 4.2 Hz, 1H), 1.49-1.33 (m, 1H), 1.09 (qd, J=9.1, 4.7 Hz, 2H), 0.86 (td, J=8.5, 5.1 Hz, 1H), 0.23 (q, J=5.0 Hz, 1H). 6 N—H exchanged with the solvent and were not observed.
    • Step 4
  • Figure US20200115354A1-20200416-C00522
    • 5-Chloro-4-(((1S,3S,4S,6R)-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 194 and making non-critical variations as required to replace rac-(3,4-trans)-7,7-dichlorobicyclo[4.1.0]heptane-3,4-diamine with rac-(3S,4S)-bicyclo[4.1.0]heptane-3,4-diamine the title compound was obtained as a white solid. Absolute stereochemistry and relative cyclopropane stereochemistry was arbitrarily assigned. LCMS (ESI) m/z: 445.1 [M+H]+. H NMR (400 MHz, DMSO-d6) δ 12.29 (br s, 1H), 7.57 (d, J=7.2 Hz, 1H), 7.18 (s, 1H), 6.84-6.64 (m, 2H), 5.97 (s, 1H), 3.14 (s, 1H), 2.73-2.61 (m, 1H), 2.54 (s, 1H), 2.15 (s, 6H), 2.01 (dd, J=12.6, 3.0 Hz, 1H), 1.80 (td, J=12.2, 5.1 Hz, 1H), 1.17 (t, J=12.3 Hz, 1H), 1.10-1.01 (m, 1H), 0.96-0.85 (m, 1H), 0.59 (td, J=8.7, 4.3 Hz, 1H), 0.12 (dd, J=9.5, 4.7 Hz, 1H).
    • Example 215
  • Figure US20200115354A1-20200416-C00523
    • 5-Chloro-4-(((1R,3R,4R,6S)-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 194 and making non-critical variations as required to replace rac-(3,4-trans)-7,7-dichlorobicyclo[4.1.0]heptane-3,4-diamine with rac-(3S,4S)-bicyclo[4.1.0]heptane-3,4-diamine the title compound was obtained as a white solid. Absolute stereochemistry and relative cyclopropane stereochemistry was arbitrarily assigned. LCMS (ESI) m/z: 445.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.77-11.45 (br s, 1H), 7.58 (d, J=7.2 Hz, 1H), 7.21 (d, J=4.4 Hz, 1H), 6.88-6.61 (m, 2H), 5.99 (d, J=3.9 Hz, 1H), 3.19 (s, 1H), 2.72-2.62 (m, 1H), 2.62-2.54 (m, 1H), 2.17 (s, 6H), 2.02 (dd, J=12.7, 3.7 Hz, 1H), 1.81 (td, J=12.2, 5.3 Hz, 1H), 1.18 (t, J=11.7 Hz, 1H), 1.12-1.02 (m, 1H), 0.92 (dt, J=8.3, 5.0 Hz, 1H), 0.60 (td, J=8.8, 4.4 Hz, 1H), 0.12 (dd, J=9.7, 4.6 Hz, 1H).
    • Example 216
  • Figure US20200115354A1-20200416-C00524
    • 5-Chloro-4-(((1R,3S,4S,6S)-4-(dimethylamino)-7,7-difluorobicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 194 and making non-critical variations as required to replace rac-(3,4-trans)-7,7-dichlorobicyclo[4.1.0]heptane-3,4-diamine with rac-(3S,4S)-bicyclo[4.1.0]heptane-3,4-diamine the title compound was obtained as a white solid. Absolute stereochemistry and relative cyclopropane stereochemistry was arbitrarily assigned. LCMS (ESI) m/z: 445.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 12.04 (br s, 1H), 7.58 (d, J=7.3 Hz, 1H), 7.21 (d, J=4.4 Hz, 1H), 6.75 (d, J=4.4 Hz, 1H), 6.71 (d, J=12.9 Hz, 1H), 5.93 (d, J=4.9 Hz, 1H), 3.25-3.16 (m, 1H), 2.85-2.72 (m, 1H), 2.37 (dd, J=13.0, 4.1 Hz, 1H), 2.26-2.21 (m, 1H), 2.19 (s, 6H), 1.71-1.57 (m, 1H), 1.46 (t, J=13.2 Hz, 1H), 1.00-0.85 (m, 2H), 0.56 (td, J=8.9, 4.5 Hz, 1H), 0.41 (q, J=5.0 Hz, 1H).
    • Example 217
  • Figure US20200115354A1-20200416-C00525
    • 5-Chloro-4-(((1S,3R,4R,6R)-4-(dimethylamino)bicyclo[4.1.0]heptan-3-yl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide
  • Following the procedure described in Example 194 and making non-critical variations as required to replace rac-(3,4-trans)-7,7-dichlorobicyclo[4.1.0]heptane-3,4-diamine with rac-(3S,4S)-bicyclo[4.1.0]heptane-3,4-diamine the title compound was obtained as a white solid. Absolute stereochemistry and relative cyclopropane stereochemistry was arbitrarily assigned. LCMS (ESI) m/z: 445.1 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 7.58 (d, J=7.3 Hz, 1H), 7.20 (d, J=4.4 Hz, 1H), 6.75 (d, J=4.4 Hz, 1H), 6.71 (d, J=12.8 Hz, 1H), 5.93 (d, J=4.7 Hz, 1H), 3.24-3.17 (m, 1H), 2.78 (td, J=11.2, 5.4 Hz, 1H), 2.38 (dd, J=13.2, 4.3 Hz, 1H), 2.22 (d, J=9.2 Hz, 1H), 2.18 (s, 6H), 1.71-1.58 (m, 1H), 1.46 (t, J=13.3 Hz, 1H), 0.91 (d, J=4.7 Hz, 2H), 0.56 (td, J=8.8, 4.4 Hz, 1H), 0.41 (q, J=5.0 Hz, 1H). Acidic NH not visible.
    • Example 218
  • Figure US20200115354A1-20200416-C00526
    • 5-chloro-4-(((1S,2S,4R)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)-cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00527
    • Step 1
  • Figure US20200115354A1-20200416-C00528
    • tert-Butyl((1S,2S,4R)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)-carbamate
  • To 20% Pd(OH)2 on charcol (700 mg, 46 mmol) was added tert-butyl N-[(1S,6S)-6-(dimethylamino)-4-[3-(trifluoromethyl)phenyl]cyclohex-3-en-1-yl]carbamate (2300 mg, 5.98 mmol) prepared in Example 181 in ethyl acetate (30 mL). The reaction is stirred at rt for 2 hours under H2 resulting in a mixture of diastereomers. Pd(OH)2 was filtered through a pad of Celite and the filtrate was concentrated. The diastereomers were separated by flash column chromatography through Si gel (0-60% MTBE/hexanes) to provide tert-butyl((1S,2S,4R)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)-carbamate (450 mg, 20% yield) as the minor diastereomer. LCMS (ESI) m/z: 387.2 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00529
    • (1S,2S,5R)—N1,N1-Dimethyl-5-(3-(trifluoromethyl)phenyl)cyclohexane-1,2-diamine
  • tert-Butyl((1S,2S,4R)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)-carbamate (200 mg, 0.52 mmol) was dissolved in 1,4-dioxane (1 mL) and to the mixture was added 4M HCl in dioxane (2 mL, 8.0 mmol). The reaction is stirred at rt for 1 h then diluted with MTBE (30 mL) and quenched with saturated aqueous Na2CO3 (15 mL). The organic layer was separated and dried over Na2SO4. The solvent was removed in vacuo to provide crude (1S,2S,5R)—N,N-dimethyl-5-(3-(trifluoromethyl)phenyl)cyclohexane-1,2-diamine (140 mg, 95% yield) which was used without any further purification. LCMS (ESI) m/z: 287.1 [M+H]+.
    • Step 3
  • Figure US20200115354A1-20200416-C00530
    • 5-Chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4R)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • In a flask containing 5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2,4-difluoro-N-pyrimidin-4-yl-benzenesulfonamide (60 mg, 0.13 mmol) was added DMF (0.50 mL), DIPEA (0.05 mL, 0.26 mmol) and (1S,2S,5R)—N1,N1-dimethyl-5-(3-(trifluoromethyl)phenyl)cyclohexane-1,2-diamine (25 mg, 0.09 mmol). After overnight stirring at 65° C., the reaction was quenched with water (10 mL). The product was extracted with EtOAc (30 mL), the organic layer was separated and washed three times with water followed by brine (5 mL). The organic layer was dried over Na2SO4, flittered and concentrated in vacuo. The residue was purified by flash column chromatography through Si gel (EtOAc/hexanes) to give 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4R)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)-cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (53 mg, 84% yield). LCMS (ESI) m/z: 722.3 [M+H]+.
    • Step 4
  • Figure US20200115354A1-20200416-C00531
    • 5-Chloro-4-(((1S,2S,4R)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)-cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • In a flask containing 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4R)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)-cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (53 mg, 0.07 mmol) was added formic acid (0.47 mL, 7.34 mmol) and the reaction is stirred at 25° C. for 3 hours. The reaction was concentrated and the product was purified directly by C18 reverse phase flash column chromatography (0-100% MeCN/10 mM aqueous ammonium formate, pH=3.8). Appropriate fractions combined and lyophilized to provide 5-chloro-4-(((1S,2S,4R)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)-cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (23 mg, 55% yield). LCMS (ESI) m/z: 572.1 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 8.44 (s, 1H), 8.14 (s, 1H), 7.82-7.42 (m, 6H), 6.83 (d, J=12.9 Hz, 1H), 6.77 (d, J=3.6 Hz, 1H), 5.83 (d, J=3.8 Hz, 1H), 3.94-3.74 (m, 2H), 3.14-2.77 (m, 2H), 2.43 (s, 6H), 2.23-2.08 (m, 1H), 2.01-1.81 (m, 3H), 1.45-1.28 (m, 1H).
    • Example 219
  • Figure US20200115354A1-20200416-C00532
    • 5-chloro-4-(((1S,2S,4S)-4-(3-cyclopropylphenyl)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00533
    Figure US20200115354A1-20200416-C00534
    • Step 1
  • Figure US20200115354A1-20200416-C00535
    • tert-Butyl((3S,4S)-3′-bromo-3-(dimethylamino)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate
  • Following the procedure as described in Example 224 and making non-critical variations as required to replace 1-(4-(trifluoromethyl)phenyl)ethanone with 1-(3-bromophenyl)ethanone, tert-butyl((3S,4S)-3′-bromo-3-(dimethylamino)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate was obtained. LCMS (ESI) m/z: 395.1 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00536
    • tert-Butyl((3S,4S)-3′-cyclopropyl-3-(dimethylamino)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate
  • In a seal tube containing tert-butyl((3S,4S)-3′-bromo-3-(dimethylamino)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate (440 mg, 1.11 mmol) in degassed toluene (22 mL) and degassed water (10.9 mL) was added cesium carbonate (1.09 g, 3.34 mmol) and potassium cyclopropyltrifluoroborate (659 mg, 4.45 mmol). The tube was bubbled with nitrogen for 1 minute then palladium acetate (38 mg, 0.17 mmol) and Butyldi-1-adamantylphosphine (120 mg, 0.33 mmol) were added. The tube was bubbled for 2 min with nitrogen then sealed and heated at 110° C. for 18 hours. The reaction mixture was cooled down then filtered trough celite, rinsed with EtOAc and the filtrate washed with saturated aqueous brine solution, dried over Na2SO4, filtered and concentrated. The crude product was purified by flash column chromatography through Si gel (0-20% MeOH/DCM) to provide tert-butyl((3S,4S)-3′-cyclopropyl-3-(dimethylamino)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate (370 mg, 93%). LCMS (ESI) m/z: 357.2 [M+H]+.
    • Step 3
  • Figure US20200115354A1-20200416-C00537
    • tert-Butyl((1S,2S)-4-(3-cyclopropylphenyl)-2-(dimethylamino)cyclohexyl)carbamate & tert-butyl((1S,2S)-2-(dimethylamino)-4-(3-propylphenyl)cyclohexyl)carbamate
  • To tert-butyl((3S,4S)-3′-cyclopropyl-3-(dimethylamino)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate (330 mg, 0.93 mmol) in ethyl acetate (3 mL) was added palladium hydroxide 20% on charcol (130 mg). The reaction was submitted to 3 cycles of hydrogen/vacuum purged and then was stirred at room temperature for 4 hours under hydrogen atmosphere. The reaction was submitted to 3 cycles of nitrogen/vacuum purged, filtered through celite, rinsed with EtOAc and concentrated to provide a mixture of tert-butyl((1S,2S)-4-(3-cyclopropylphenyl)-2-(dimethylamino)cyclohexyl)carbamate and tert-butyl((1S,2S)-2-(dimethylamino)-4-(3-propylphenyl)cyclohexyl)carbamate (280 mg, 85%). LCMS (ESI) m/z: 359.3 [M+H]+. The mixture was used without further purification in the subsequent step.
    • Step 4
  • Figure US20200115354A1-20200416-C00538
    • (1S,2S,5S)-5-(3-Cyclopropylphenyl)-N1,N1-dimethylcyclohexane-1,2-diamine & (1S,2S,5S)—N1,N1-dimethyl-5-(3-propylphenyl)cyclohexane-1,2-diamine
  • To the mixture of tert-butyl((1S,2S)-4-(3-cyclopropylphenyl)-2-(dimethylamino)cyclohexyl)carbamate and tert-butyl((1S,2S)-2-(dimethylamino)-4-(3-propylphenyl)cyclohexyl)carbamate (280 mg, 0.78 mmol) in DCM (4 mL) was added HCl 4 N in dioxane (6 mL, 23 mmol). The reaction was stirred at room temperature for 60 minutes, concentrated and purified directly by C18 reverse phase flash chromatography (0-40% MeCN/10 mM aqueous ammonium formate, pH=3.8). Appropriate fractions combined and lyophilized to provide a mixture of (1S,2S,5S)-5-(3-cyclopropylphenyl)-N1,N1-dimethylcyclohexane-1,2-diamine and (1S,2S,5S)—N1,N1-dimethyl-5-(3-propylphenyl)cyclohexane-1,2-diamine (51 mg, 22%).
    • Step 5
  • Figure US20200115354A1-20200416-C00539
    • 5-Chloro-4-(((1S,2S,4S)-4-(3-cyclopropylphenyl)-2-(dimethylamino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide & 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-propylphenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • To the mixture of (1S,2S,5S)-5-(3-cyclopropylphenyl)-N1,N1-dimethylcyclohexane-1,2-diamine and (1S,2S,5S)—N1,N1-dimethyl-5-(3-propylphenyl)cyclohexane-1,2-diamine (51 mg, 0.2 mmol) in DMF (1 mL) was added DIPEA (0.14 mL, 0.79 mmol) and 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide (108 mg, 0.24 mmol). The reaction mixture was stirred at 65° C. for 18 hours and then quenched with water (10 mL). The product was extracted with EtOAc (50 mL), the organic layer was separated, washed with water, saturated aqueous brine solution dried over Na2SO4, filtered and concentrated. The crude product was purified by flash column chromatography through Si gel (0-20% MeOH/DCM) to provide a mixture of 5-chloro-4-(((1S,2S,4S)-4-(3-cyclopropylphenyl)-2-(dimethylamino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide and 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-propylphenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (105 mg, 77%). LCMS (ESI) m/z: 694.3 [M+H]
    • Step 6
  • Figure US20200115354A1-20200416-C00540
    • 5-Chloro-4-(((1S,2S,4S)-4-(3-cyclopropylphenyl)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • To the mixture of 5-chloro-4-(((1S,2S,4S)-4-(3-cyclopropylphenyl)-2-(dimethylamino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide and 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-propylphenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (105 mg, 0.15 mmol) was added formic acid (1 mL, 26.5 mmol). The reaction was stirred at room temperature for 3 hours then concentrated. The crude was dissolved in a mixture of DMF and DMSO, filtered and purified by prep-HPLC (CSH C18 col, 5 m, 30×75 mm at 40° C.; 45 mL/min, 25-45% MeCN/10 mM aqueous ammonium formate, pH=3.8). Appropriate fractions combined and lyophilized to provide 5-chloro-4-(((1S,2S,4S)-4-(3-cyclopropylphenyl)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (48 mg, 58%). LCMS (ESI) m/z: 544.3 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.34 (s, 1H), 8.15 (s, 1H), 8.01 (d, J=5.0 Hz, 1H), 7.64 (d, J=7.3 Hz, 1H), 7.17 (t, J=7.6 Hz, 1H), 7.12-6.97 (m, 2H), 6.88 (d, J=7.7 Hz, 1H), 6.74 (d, J=12.8 Hz, 1H), 6.63 (d, J=5.1 Hz, 1H), 5.81 (d, J=5.9 Hz, 1H), 2.67-2.55 (m, 1H), 2.35 (s, 6H), 2.14 (dd, J=12.8, 3.1 Hz, 1H), 1.99 (d, J=12.2 Hz, 1H), 1.94-1.82 (m, 1H), 1.69 (dt, J=10.3, 5.1 Hz, 2H), 1.56 (q, J=12.3 Hz, 1H), 1.46-1.27 (m, 1H), 0.99-0.88 (m, 2H), 0.71-0.59 (m, 2H). Exchangeable NH hydrogens not observed.
    • Example 220
  • Figure US20200115354A1-20200416-C00541
    • 4-(((1S,2S,4S)-2-(Dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2,5-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00542
    • Step 1
  • Figure US20200115354A1-20200416-C00543
    • N-(2,4-Dimethoxybenzyl)-2,4,5-trifluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • A solution of N-[(2,4-dimethoxyphenyl)methyl]pyrimidin-4-amine (1.0 g, 4.1 mmol) and 2,4,5-trifluorobenzenesulfonyl chloride (1.41 g, 6.12 mmol) and 1,4-diazabicyclo[2.2.2]octane (0.69 g, 6.12 mmol) in MeCN (20 mL) was stirred at rt for 5 h. The reaction was filtered to remove the white solid, then concentrated, and purified by flash column chromatography through Si gel (EtOAc/hexanes) to provide N-(2,4-dimethoxybenzyl)-2,4,5-trifluoro-N-(pyrimidin-4-yl)benzenesulfonamide (1.2 g, 67% yield) as a pale yellow solid. LCMS (ESI) m/z: 440.1 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00544
    • N-(2,4-Dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2,5-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • In a flask containing N-(2,4-dimethoxybenzyl)-2,4,5-trifluoro-N-(pyrimidin-4-yl)benzenesulfonamide (184 mg, 0.42 mmol) was added DMF (2 mL), DIPEA (0.15 mL, 0.84 mmol) and (1S,2S,4S)—N1,N1-dimethyl-4-[3-(trifluoromethyl)phenyl]cyclohexane-1,2-diamine (80 mg, 0.28 mmol). After overnight stirring at 65° C., the reaction was quenched with water (10 mL). The product was extracted with EtOAc (30 mL), the organic layer was separated and washed with three times with water and followed by brine (5 mL). The organic layer was dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by flash column chromatography through Si gel (EtOAc/hexanes) to provide N-(2,4-dimethoxybenzyl)-4-(((1S,2S, 4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2,5-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide (140 mg, 71% yield). LCMS (ESI) m/z: 706.4 [M+H]+.
    • Step 3
  • Figure US20200115354A1-20200416-C00545
    • 4-(((1S,2S,4S)-2-(Dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2,5-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 115 and making non-critical variations as required using N-(2,4-dimethoxybenzyl)-4-(((1S,2S, 4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2,5-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide (185 mg, 0.26 mmol), 4-(((1S,2S, 4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2,5-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide (73 mg, 50% yield) was obtained. LCMS (ESI) m/z: 556.3 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 8.37 (s, 1H), 8.03 (d, J=6.0 Hz, 1H), 7.59 (dd, J=26.9, 12.1 Hz, 4H), 7.38 (dd, J=11.1, 6.6 Hz, 1H), 6.74 (dd, J=12.3, 6.9 Hz, 1H), 6.68 (d, J=5.8 Hz, 1H), 6.10 (s, 1H), 3.69 (m, 1H), 3.22-3.20 (m, 1H), 2.80 (m, 1H), 2.45 (s, 6H), 2.08 (m, 2H), 1.66 (m, 3H), 1.36 (s, 1H). Acidic NH not observed
    • Example 221
  • Figure US20200115354A1-20200416-C00546
    • 5-Chloro-2-fluoro-4-(((1S,2S)-2-((R)-3-fluoro-3-(hydroxymethyl)piperidin-1-yl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 212 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide, 5-Chloro-2-fluoro-4-(((1S,2S)-2-((R)-3-fluoro-3-(hydroxymethyl)piperidin-1-yl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide was obtained. LCMS (ESI) m/z: 516.2 [(M+H]. 1H NMR (400 MHz, d6-dmso) δ 12.54 (s, 1H), 8.55 (s, 1H), 8.26 (d, J=6.0 Hz, 1H), 7.69 (d, J=7.4 Hz, 1H), 6.91 (d, J=6.1 Hz, 1H), 6.67 (d, J=13.1 Hz, 1H), 5.98 (s, 1H), 4.77 (s, 1H), 3.47 (dd, J=23.9, 12.3 Hz, 2H), 3.28-3.14 (m, 3H), 2.63-2.55 (m, 1H), 2.46-2.41 (m, 1H), 2.32-2.22 (m, 1H), 2.15 (d, J=11.2 Hz, 1H), 1.81 (d, J=7.5 Hz, 1H), 1.78-1.71 (m, 1H), 1.69-1.54 (m, 2H), 1.52-1.43 (m, 2H), 1.43-1.30 (m, 2H), 1.29-1.16 (m, 2H), 1.11 (q, J=10.0 Hz, 1H).
    • Example 222
  • Figure US20200115354A1-20200416-C00547
    • 5-Chloro-2-fluoro-4-(((1S,2S)-2-((S)-3-fluoro-3-(hydroxymethyl)piperidin-1-yl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 212 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide, 5-Chloro-2-fluoro-4-(((1S,2S)-2-((S)-3-fluoro-3-(hydroxymethyl)piperidin-1-yl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide was obtained. LCMS (ESI) m/z: 516.2 [M+H]+. 1H NMR (400 MHz, d6-dmso) δ 12.56 (s, 1H), 8.57 (s, 1H), 8.29 (d, J=5.9 Hz, 1H), 7.69 (d, J=7.4 Hz, 1H), 6.94 (d, J=6.1 Hz, 1H), 6.69 (d, J=13.2 Hz, 1H), 6.00 (s, 1H), 4.81 (s, 1H), 3.46 (dd, J=24.1, 5.5 Hz, 2H), 3.30-3.20 (m, 2H), 2.59 (t, J=11.8 Hz, 2H), 2.45 (d, J=10.6 Hz, 1H), 2.22 (t, J=8.4 Hz, 1H), 2.13 (d, J=10.8 Hz, 1H), 1.82 (d, J=8.2 Hz, 1H), 1.78-1.71 (m, 1H), 1.71-1.56 (m, 2H), 1.56-1.45 (m, 1H), 1.45-1.29 (m, 2H), 1.29-1.05 (m, 4H).
    • Example 223
  • Figure US20200115354A1-20200416-C00548
    • 5-Chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-propylphenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 219 step 6, 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-propylphenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide was obtained. LCMS (ESI) m/z: 546.3 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.22 (s, 1H), 7.88 (s, J=52.6 Hz, 1H), 7.57 (d, J=7.3 Hz, 1H), 7.20 (t, J=7.9 Hz, 1H), 7.11 (d, J=6.0 Hz, 2H), 6.99 (d, J=7.4 Hz, 1H), 6.57 (d, J=12.5 Hz, 1H), 6.48 (d, J=5.3 Hz, 1H), 5.32 (d, J=5.0 Hz, 1H), 2.58-2.49 (m, 2H), 2.41 (t, J=9.0 Hz, 1H), 2.21 (s, 7H), 2.17 (s, 1H), 2.14-1.86 (m, 4H), 1.86-1.68 (m, 2H), 1.63-1.48 (m, 2H), 1.48-1.28 (m, 1H), 0.87 (t, J=7.3 Hz, 3H).
    • Example 224
  • Figure US20200115354A1-20200416-C00549
    • 5-Chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(4-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00550
    Figure US20200115354A1-20200416-C00551
    • Step 1
  • Figure US20200115354A1-20200416-C00552
    • 1-(Prop-1-en-2-yl)-4-(trifluoromethyl)benzene
  • To methyltriphenylphosphonium bromide (14.2 g, 39.9 mmol) was added THF (44 mL). The suspension was cooled to 0° C. and potassium tert-butoxide (4.47 g, 39.9 mmol) was added. The resulting yellow suspension was stirred at 0° C. for 45 minutes. To the suspension was added a solution of 1-(4-(trifluoromethyl)phenyl)ethanone (5.0 g, 26.6 mmol) in THF (22 mL) dropwise. The resulting mixture was then brought up to room temperature slowly and stirred for 16 hours. Reaction mixture was diluted with hexanes (150 mL) and stirred for 20 min. The resulting precipitate was filtered, rinsed with hexane and the filtrate was concentrate. More hexanes (200 mL) was added to the residue and stirred for 20 min. The resulting precipitate was filtered through celite and the filtrate was concentrated to provide 1-(prop-1-en-2-yl)-4-(trifluoromethyl)benzene (3.98 g, 80%) 1H NMR (400 MHz, CDCl3) δ 7.61-7.53 (m, 4H), 5.44 (s, 1H), 5.22-5.17 (m, 1H), 2.17 (dd, J=1.3, 0.7 Hz, 3H).
  • Figure US20200115354A1-20200416-C00553
    • 1-(3-Iodoprop-1-en-2-yl)-4-(trifluoromethyl)benzene
  • To iodine (33.9 g, 133.6 mmol) in DMA (180 mL) was added 1-(prop-1-en-2-yl)-4-(trifluoromethyl)benzene (4.98 g, 26.8 mmol) in DMA (5 mL). The reaction was stirred at room temperature for 20 minutes. A freshly made aqueous solution of saturated aqueous sodium thiosulfate was added as well as EtOAc (250 mL). The resulting solution was stirred for 10 minutes, water phase was removed and organic phase washed with water (2×50 mL) and with saturated aqueous brine solution (100 mL, then 50 mL) dried over MgSO4, filtered and concentrated. The crude product was purified by flash column chromatography through Si gel (100% Hexanes) to provide 1-(3-iodoprop-1-en-2-yl)-4-(trifluoromethyl)benzene (2.58 g, 31%). H NMR (400 MHz, CDCl3) δ 7.64 (d, J=8.3 Hz, 2H), 7.56 (d, J=8.2 Hz, 2H), 5.63 (s, 1H), 5.53 (s, 1H), 4.32 (s, 2H).
    • Step 3
  • Figure US20200115354A1-20200416-C00554
    • (4S,5S)-tert-Butyl 4-allyl-6-oxo-5-(2-(4-(trifluoromethyl)phenyl)allyl)-1,3-oxazinane-3-carboxylate
  • A solution of (S)-tert-butyl 4-allyl-6-oxo-1,3-oxazinane-3-carboxylate (2.0 g, 8.3 mmol) in THF (80 mL) was cooled to −78° C. under nitrogen. KHMDS (0.5 M in Toluene) (16.6 mL, 8.3 mmol) was then added dropwise and the solution was left to stir at −78° C. for 30 minutes. 1-(3-iodoprop-1-en-2-yl)-4-(trifluoromethyl)benzene (2.07 g, 6.63 mmol) as a solution in THF (5 mL) was then added at once quickly and stirring was continued for 4.5 hours at −78° C. The reaction was quenched with saturated aqueous NH4Cl solution (40 mL) at −78° C. and then water (40 mL) and EtOAc (200 mL) were added. The organic layer was separated and washed with brine (2×40 mL) and then dried over Na2SO4 filtered and concentrated. The crude product was purified by flash column chromatography through Si gel (EtOAc/Hexanes) to provide (4S,5S)-tert-butyl 4-allyl-6-oxo-5-(2-(4-(trifluoromethyl)phenyl)allyl)-1,3-oxazinane-3-carboxylate (1.29 g, 37%) as a clear oil. LCMS (ESI) m/z: 326.1 [M-Boc+H]+
    • Step 4
  • Figure US20200115354A1-20200416-C00555
    • (4aS,8aS)-tert-Butyl 4-oxo-6-(4-(trifluoromethyl)phenyl)-2,4,4a,5,8,8a-hexahydro-1H-benzo[d][1,3]oxazine-1-carboxylate
  • (4S,5S)-tert-Butyl 4-allyl-6-oxo-5-(2-(4-(trifluoromethyl)phenyl)allyl)-1,3-oxazinane-3-carboxylate (1.1 g, 2.57 mmol) was dissolved in toluene (50 mL) and to this solution was added Grubbs metal second generation (328 mg, 0.39 mmol). The reaction mixture was stirred at 65° C. for 6 hours. The reaction was cooled down to rt and purified directly by flash column chromatography through Si gel (EtOAc/Hexanes) to provide (4aS,8aS)-tert-butyl 4-oxo-6-(4-(trifluoromethyl)phenyl)-2,4,4a,5,8,8a-hexahydro-1H-benzo[d][1,3]oxazine-1-carboxylate (926 mg, 90%). LCMS (ESI) m/z: 298.1 [M-Boc+H]
    • Step 5
  • Figure US20200115354A1-20200416-C00556
    • (3S,4S)-4-((tert-Butoxycarbonyl)amino)-4′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-3-carboxylic Acid
  • (4aS,8aS)-tert-Butyl 4-oxo-6-(4-(trifluoromethyl)phenyl)-2,4,4a,5,8,8a-hexahydro-1H-benzo[d][1,3]oxazine-1-carboxylate (920 mg, 2.32 mmol) was dissolved in THF (12 mL) and to the mixture was added a solution of lithium hydroxide (1.7 g, 69.4 mmol) in water (12.3 mL). The mixture was stirred at room temperature for 18 hours then acidified carefully with a 6 M aqueous solution of HCl to pH˜3. The product was extracted by EtOAc (200 mL) and the organic layer was washed with saturated aqueous brine solution and dried over Na2SO4. The solvent was removed in vacuo to afford (3S,4S)-4-((tert-butoxycarbonyl)amino)-4′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-3-carboxylic acid (845 mg, 95%) and was used without further purification in the subsequent step. LCMS (ESI) m/z: 384.1 [M−H]−.
    • Step 6
  • Figure US20200115354A1-20200416-C00557
    • tert-Butyl((3S,4S)-3-isocyanato-4′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate
  • To (3S,4S)-4-((tert-butoxycarbonyl)amino)-4′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-3-carboxylic acid (845 mg, 2.19 mmol) in toluene (6.2 mL) was added triethylamine (0.43 mL, 3.06 mmol) and diphenylphosphoryl azide (0.52 mL, 2.41 mmol). The mixture was heated to 100° C. for 35 minutes then cooled down to room temperature and purified directly by flash column chromatography through Si gel (EtOAc/Hexanes) to provide tert-butyl((3S,4S)-3-isocyanato-4′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate (616 mg, 73%).
    • Step 7
  • Figure US20200115354A1-20200416-C00558
    • tert-Butyl((3S,4S)-3-amino-4′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate
  • To tert-butyl((3S,4S)-3-isocyanato-4′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate (615 mg, 1.60 mmol) in THF (3 mL) was added a solution of potassium trimethylsilanolate (248 mg, 1.93 mmol) in THF (1 mL). The mixture was stirred at room temperature for 18 hours then quenched by addition of saturated aqueous NaHCO3 (20 mL) and the product was extracted with EtOAc (100 mL). The organic layer was separated, dried over Na2SO4, filtered and concentrated to provide tert-butyl((3S,4S)-3-amino-4′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate (507 mg, 88% yield) and was used without further purification in the subsequent step. LCMS (ESI) m/z: 357.1 [M+H]+.
    • Step 8
  • Figure US20200115354A1-20200416-C00559
    • tert-Butyl((3S,4S)-3-(dimethylamino)-4′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate
  • To tert-butyl((3S,4S)-3-amino-4′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate (505 mg, 1.42 mmol) in methanol (4.7 mL) at 0° C. was added formaldehyde (37% w/w in H2O) (1.06 mL, 14.2 mmol) followed by sodium cyanoborohydride (214 mg, 5.7 mmol). The reaction mixture was stirred for 1 hour at room temperature and then diluted with saturated aqueous NaHCO3 (50 mL) and EtOAc (200 mL) and the phases were separated. The organic layer was washed with saturated aqueous brine solution, dried over Na2SO4, filtered and concentrated. The crude product was purified by flash column chromatography through Si gel (MeOH/DCM) to provide tert-butyl((3S,4S)-3-(dimethylamino)-4′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate (422 mg, 77%). LCMS (ESI) m/z: 385.2 [M+H]+.
    • Step 9
  • Figure US20200115354A1-20200416-C00560
    • tert-Butyl((1S,2S,4S)-2-(dimethylamino)-4-(4-(trifluoromethyl)phenyl)cyclohexyl)carbamate
  • To tert-butyl((3S,4S)-3-(dimethylamino)-4′-(trifluoromethyl)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate (420 mg, 1.09 mmol) in ethyl acetate (5.5 mL) was added palladium hydroxide 20% on charcol (130 mg). The reaction was submitted to 3 cycles of hydrogen/vacuum purged and then was stirred at room temperature for 4 hours under hydrogen atmosphere. The reaction was submitted to 3 cycles of nitrogen/vacuum purged, filtered through celite, rinsed with EtOAc and concentrated to provide tert-butyl((1S,2S,4S)-2-(dimethylamino)-4-(4-(trifluoromethyl)phenyl)cyclohexyl)carbamate as a mixture of diastereoisomeres (323 mg, 77%). LCMS (ESI) m/z: 387.2 [M+H]+. The mixture was used without further purification in the subsequent step.
    • Step 10
  • Figure US20200115354A1-20200416-C00561
    • (1S,2S,5S)—N1,N1-Dimethyl-5-(4-(trifluoromethyl)phenyl)cyclohexane-1,2-diamine
  • To tert-butyl((1S,2S,4S)-2-(dimethylamino)-4-(4-(trifluoromethyl)phenyl)cyclohexyl)carbamate (300 mg, 0.78 mmol) in DCM (2.6 mL) was added 4 N HCl in dioxane (1 mL, 4 mmol). The reaction was stirred at room temperature for 2 h then concentrated and purified by C18 reverse phase flash chromatography (5-35% MeCN/10 mM aqueous ammonium formate, pH=3.8). Appropriate fractions combined and lyophilized to provide (1S,2S,5S)—N1,N1-dimethyl-5-(4-(trifluoromethyl)phenyl)cyclohexane-1,2-diamine (93 mg, 42%).
    • Step 11
  • Figure US20200115354A1-20200416-C00562
    • 5-Chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(4-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • To (1S,2S,5S)—N1,N1-dimethyl-5-(4-(trifluoromethyl)phenyl)cyclohexane-1,2-diamine (100 mg, 0.35 mmol) in DMF (1.7 mL) were added DIPEA (0.19 mL, 1.05 mmol) and 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide (239 mg, 0.52 mmol). The reaction mixture was stirred at 65° C. for 18 hours and then quenched with water (10 mL). The product was extracted with EtOAc (50 mL), the organic layer was separated, washed with water, saturated aqueous brine solution dried over Na2SO4, filtered and concentrated. The crude product was purified by flash column chromatography through Si gel (0-20% MeOH/DCM) to provide 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(4-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (250 mg, 99%). LCMS (ESI) m/z: 722.3, 724.3 [M+H]+.
    • Step 12
  • Figure US20200115354A1-20200416-C00563
    • 5-Chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(4-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 115 step 2 and making non-critical variations using 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(4-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (250 mg, 99%), 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(4-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (91 mg, 46%) was obtained. LCMS (ESI) m/z: 572.2, 574.2 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 8.42 (s, 1H), 8.10 (d, J=5.4 Hz, 1H), 7.69 (t, J=7.5 Hz, 4H), 7.53 (d, J=8.2 Hz, 2H), 6.82 (d, J=12.9 Hz, 1H), 6.74 (d, J=5.9 Hz, 1H), 5.96 (d, J=7.1 Hz, 1H), 3.70 (s, 1H), 2.79 (t, J=11.6 Hz, 1H), 2.41 (s, 7H), 2.16-2.00 (m, 2H), 1.83-1.57 (m, 3H), 1.48-1.33 (m, 1H).
    • Example 225
  • Figure US20200115354A1-20200416-C00564
    • 5-Chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethoxy)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 224 and making non-critical variations as required to replace 1-(4-(trifluoromethyl)phenyl)ethanone with 1-(3-(trifluoromethoxy)phenyl)ethanone, 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethoxy)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide was obtained. LCMS (ESI) m/z: 588.4, 590.3 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 8.40 (s, 1H), 8.08 (d, J=5.5 Hz, 1H), 7.65 (d, J=7.4 Hz, 1H), 7.45 (t, J=7.9 Hz, 1H), 7.33 (d, J=7.9 Hz, 1H), 7.28 (s, 1H), 7.23-7.17 (m, 1H), 6.79 (d, J=13.0 Hz, 1H), 6.71 (d, J=5.7 Hz, 1H), 5.94 (d, J=7.1 Hz, 1H), 3.69 (s, 1H), 2.74 (t, J=12.0 Hz, 1H), 2.41 (s, 7H), 2.07 (s, 2H), 1.79-1.54 (m, 3H), 1.46-1.27 (m, 1H). Acidic NH not observed.
    • Example 226
  • Figure US20200115354A1-20200416-C00565
    • 5-Chloro-2-fluoro-4-(((1S,2S,4S)-2-(methylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00566
    • Step 1
  • Figure US20200115354A1-20200416-C00567
    • 5-Chloro-2-fluoro-4-(((1S,2S,4S)-2-(methylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide
  • To a stirred solution of 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S, 4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (60 mg, 0.10 mmol) in a mixture of chloroform (0.5 mL) and IPA (0.2 mL) was added 3-chloroperbenzoic acid (mCPBA) (40 mg, 0.18 mmol). The reaction was stirred for 15 min before addition of concentrated HCl (50 uL, 0.10 mmol) and ferrocene (31 mg, 0.16 mmol). The mixture was then stirred at 50° C. overnight. The mixture was diluted in DMSO (5 mL) and passed through a C18 reverse phase column to remove the catalyst. The filtrate was concentrated and purified by prep-HPLC (CSH column, MeCN and 10 mM AmBicarbonate in water as eluent, from 20% MeCN to 40% MeCN) to provide 5-chloro-2-fluoro-4-(((1S,2S, 4S)-2-(methylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-N-(pyrimidin-4-yl)benzenesulfonamide (8 mg, 14% yield). LCMS (ESI) m/z: 558.4 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 8.25 (s, 1H), 7.89 (d, J=5.3 Hz, 1H), 7.67-7.49 (m, 5H), 6.67 (d, J=12.2 Hz, 1H), 6.51 (d, J=5.7 Hz, 1H), 5.76 (s, 1H), 2.79 (m, 1H), 2.41 (s, 3H), 2.18 (m, 1H), 1.99 (m, 1H), 1.76 (m, 2H), 1.55 (m, 1H), 1.43 (m, 1H), 1.22 (m, 1H). Exchangeable NH hydrogens not observed.
    • Example 227
  • Figure US20200115354A1-20200416-C00568
    • 5-Bromo-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00569
    • Step 1
  • Figure US20200115354A1-20200416-C00570
    • 5-Bromo-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 220 and making non-critical variations as necessary to replace 2,4,5-trifluorobenzenesulfonyl chloride with 5-bromo-2,4-difluorobenzene-1-sulfonyl chloride, 5-bromo-N-(2,4-dimethoxybenzyl)-4-(((1S,2S, 4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide was obtained. LCMS (ESI) m/z: 768.3 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00571
    • 5-Bromo-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 115 and making non-critical variations as required using 5-bromo-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide, 5-bromo-4-(((1S,2S, 4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide was obtained. LCMS (ESI) m/z: 616.3 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 8.40 (s, 1H), 8.07 (s, 1H), 7.81 (d, J=7.6 Hz, 1H), 7.65-7.54 (m, 4H), 6.73 (m, 2H), 5.77 (d, J=6.7 Hz, 1H), 3.62 (m, 1H), 2.81 (m, 1H), 2.36 (s, 6H), 2.09 (dd, J=37.6, 11.8 Hz, 2H), 1.74 (m, 2H), 1.68-1.55 (m, 1H), 1.35 (m, 1H). Exchangeable NH hydrogens not observed.
    • Example 228 & Example 229
  • Figure US20200115354A1-20200416-C00572
    • 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(isoxazol-3-yl)benzenesulfonamide & 5-chloro-4-(((1S,2S,4R)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(isoxazol-3-yl)benzenesulfonamide Step 1
  • Figure US20200115354A1-20200416-C00573
    • 5-chloro-4-(((1S,2S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(isoxazol-3-yl)benzenesulfonamide
  • Following the procedure described in Example 181 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(isoxazol-3-yl)benzenesulfonamide, 5-chloro-4-(((1S,2S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(isoxazol-3-yl)benzenesulfonamide was obtained as a white solid. LCMS (ESI) m/z: 561.2 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00574
    • 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(isoxazol-3-yl)benzenesulfonamide & 5-chloro-4-(((1S,2S,4R)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(isoxazol-3-yl)benzenesulfonamide
  • 5-chloro-4-(((1S,2S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(isoxazol-3-yl)benzenesulfonamide (90 mg) was separated by using chiral SFC (Chiralpak IC (250 mm*30 mm, 10 um), Supercritical CO2/EtOH+0.1% NH4OH=65/35; 60 mL/min) to give 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(isoxazol-3-yl)benzenesulfonamide (27 mg, first peak) as a white solid and 5-chloro-4-(((1S,2S,4R)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(isoxazol-3-yl)benzenesulfonamide (11 mg, second peak) as a white solid. Example 228: LCMS (ESI) m/z: 561.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.35 (s, 1H), 7.65 (s, 1H), 7.64-7.61 (m, 1H), 7.60-7.55 (m, 3H), 6.76 (d, J=12.4 Hz, 1H), 6.16 (s, 1H), 5.86-5.81 (m, 1H), 3.65-3.51 (m, 1H), 3.21-3.15 (m, 1H), 2.87-2.76 (m, 1H), 2.38 (s, 3H), 2.38 (s, 3H), 2.16-2.03 (m, 2H), 1.79-1.71 (m, 2H), 1.69-1.56 (m, 1H), 1.45-1.35 (m, 1H). Acidic NH hydrogen not observed. Example 229: LCMS (ESI) m/z: 561.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.62 (s, 1H), 7.73-7.72 (m, 1H), 7.67-7.60 (m, 3H), 7.45-7.26 (m, 1H), 7.03 (d, J=12.4 Hz, 1H), 6.31 (s, 1H), 6.26-6.18 (m, 1H), 4.06-3.94 (m, 1H), 3.55-3.45 (m, 1H), 2.61 (s, 3H), 2.61 (s, 3H), 2.26-2.22 (m, 1H), 1.94-1.88 (m, 2H), 1.55-1.43 (m, 2H), 1.38-1.32 (m, 2H). Acidic NH hydrogen not observed.
    • Example 230
  • Figure US20200115354A1-20200416-C00575
    • 5-Cyclopropyl-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide formate
  • Figure US20200115354A1-20200416-C00576
    • Step 1
  • Figure US20200115354A1-20200416-C00577
    • 5-cyclopropyl-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • To a solution of 5-bromo-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide prepared in Example 227 (130 mg, 0.17 mmol) in a mixture of N2 degassed toluene (2.3 mL) and H2O (1.15 mL) was added Cesium carbonate (167 mg, 0.51 mmol), Potassium cyclopropyltrifluoroborate (100 mg, 0.68 mmol), Pd(OAc)2 (5.7 mg, 0.03 mmol), and butyl di-1-adamantylphosphine (18 mg, 0.05 mmol) in that order. The vessel was capped and N2 purged 2 min before being placed in a 110° C. oil bath overnight. After 16 h, the mixture was diluted with EtOAc and H2O and the phases were separated. The organic extract was washed with saturated aqueous NaCl, dried (Na2SO4), filtered and concentrated in vacuo. Purification by flash chromatography through Si gel (0-60% EtOAc/CH2Cl2) provided 5-cyclopropyl-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (122 mg, 85%). LCMS (ESI) m/z: 728.4 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00578
    • 5-cyclopropyl-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide formate
  • Following the procedure described in Example 187 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-cyclopropyl-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (122 mg, 0.17 mmol), 5-cyclopropyl-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide formate was obtained (64 mg, 61%) as a white solid. LCMS (ESI) m/z: 578.4 [M+H]+. 1H NMR (400 MHz, d6-DMSO) [missing thiazole N—H signal] δ 8.50 (s, 1H), 8.21 (s, 1H), 8.14 (s, 1H), 7.73-7.51 (m, 4H), 7.39 (d, J=8.3 Hz, 1H), 6.84 (d, J=6.6 Hz, 1H), 6.53 (d, J=13.5 Hz, 1H), 6.01 (s, 1H), 3.08-2.91 (m, 2H), 2.91-2.76 (m, 1H), 2.42-2.17 (m, 7H), 2.01 (d, J=13.4 Hz, 1H), 1.84-1.70 (m, 2H), 1.61 (q, J=11.1 Hz, 2H), 1.50-1.17 (m, 1H), 0.92 (d, J=7.4 Hz, 2H), 0.47 (d, J=79.5 Hz, 1H). Acidic NH hydrogen not observed.
    • Example 231
  • Figure US20200115354A1-20200416-C00579
    • 5-Chloro-4-(((1S,2S,4S)-4-(3-chlorophenyl)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide formate
  • Figure US20200115354A1-20200416-C00580
    Figure US20200115354A1-20200416-C00581
    • Step 1
  • Figure US20200115354A1-20200416-C00582
    • tert-Butyl((3S,4S)-3′-chloro-3-(dimethylamino)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate
  • To tert-butyl((3S,4S)-3′-bromo-3-(dimethylamino)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate (230 mg, 0.58 mmol) in Ethanol (8.3 mL) was added tetrabutyl ammonium chloride (1.72 g, 5.82 mmol), L-Proline (402 mg, 3.49 mmol) and copper (I) oxide (250 mg, 1.75 mmol) under nitrogen purged. The vial was sealed and heated at 105° C. for 18 hours. The reaction was cooled to room temperature, poured in EtOAc, washed with aqueous NH4OH solution, water and brine, dried over Na2SO4, filtered and concentrated. The crude product was purified by flash column chromatography through Si gel (0-20% MeOH/DCM) to provide tert-butyl((3S,4S)-3′-chloro-3-(dimethylamino)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate (111 mg, 54%). LCMS (ESI) m/z: 351.1 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 7.33 (s, 1H), 7.25-7.17 (m, 3H), 6.03-5.94 (m, 1H), 5.59 (s, 1H), 3.54 (bs, 1H), 3.13 (d, J=18.3 Hz, 1H), 2.83-2.71 (m, 1H), 2.51-2.40 (m, 2H), 2.31 (s, 6H), 2.14-1.99 (m, 1H), 1.47 (s, 9H).
    • Step 2
  • Figure US20200115354A1-20200416-C00583
    • tert-Butyl((1S,2S,4S)-4-(3-chlorophenyl)-2-(dimethylamino)cyclohexyl)carbamate & tert-butyl((1S,2S,4S)-2-(dimethylamino)-4-phenylcyclohexyl)carbamate
  • To tert-butyl((3S,4S)-3′-chloro-3-(dimethylamino)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate (116 mg, 0.33 mmol) in Ethyl acetate (3.3 mL) was added Pd/C (44 mg). The reaction was submitted to 3 cycle of hydrogen/vacuum purged and then was stirred at room temperature for 20 hours under H2. The reaction was submitted to 3 cycle of nitrogen/vacuum purging, filtered through celite, rinsed with EtOAc and concentrated to provide a mixture of tert-butyl((1S,2S,4S)-4-(3-chlorophenyl)-2-(dimethylamino)cyclohexyl)carbamate and tert-butyl((1S,2S,4S)-2-(dimethylamino)-4-phenylcyclohexyl)carbamate (110 mg, 94%) which was used without further purification in the subsequent step. tert-Butyl((1S,2S,4S)-4-(3-chlorophenyl)-2-(dimethylamino)cyclohexyl)carbamate is the major species. LCMS (ESI) m/z: 353.2 [M+H]+.
    • Step 3
  • Figure US20200115354A1-20200416-C00584
    • (1S,2S,5S)-5-(3-chlorophenyl)-N1,N1-dimethylcyclohexane-1,2-diamine hydrochloride & (1S,2S,5S)—N1,N1-dimethyl-5-phenylcyclohexane-1,2-diamine hydrochloride
  • To a mixture of tert-butyl((1S,2S,4S)-4-(3-chlorophenyl)-2-(dimethylamino)cyclohexyl)carbamate and tert-butyl((1S,2S,4S)-2-(dimethylamino)-4-phenylcyclohexyl)carbamate (160 mg, 0.45 mmol) was added 4 N HCl in dioxane (2 mL, 8 mmol) and the solution was stirred at room temperature for 30 minutes then concentrated to dryness to provide a mixture of (1S,2S,5S)-5-(3-chlorophenyl)-N1,N1-dimethylcyclohexane-1,2-diamine hydrochloride and (1S,2S,5S)—N1,N1-dimethyl-5-phenylcyclohexane-1,2-diamine hydrochloride (130 mg, quant.) which was used directly in the next step without further purification.
    • Step 4
  • Figure US20200115354A1-20200416-C00585
    • 5-Chloro-4-(((1S,2S,4S)-4-(3-chlorophenyl)-2-(dimethylamino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide & 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-phenylcyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • To a flask containing a mixture of (1S,2S,5S)-5-(3-chlorophenyl)-N1,N1-dimethylcyclohexane-1,2-diamine hydrochloride and (1S,2S,5S)—N1,N1-dimethyl-5-phenylcyclohexane-1,2-diamine hydrochloride (130 mg, 0.45 mmol), was added DMF (2.5 mL) followed by DIPEA (403 uL, 2.27 mmol) and 5-chloro-N-(2,4-dimethoxybenzyl)-2,4-difluoro-N-(pyrimidin-4-yl)benzenesulfonamide (227 mg, 0.50 mmol). The reaction was stirred at 65° C. overnight then the reaction was stopped by quenching with water. The product was extracted with EtOAc, the organic layer was separated and washed with water and brine. The organic layer was dried over Na2SO4, filtered and concentrated. The crude product was purified by flash column chromatography through Si gel (EtOAc/DCM) to provide a mixture of 5-Chloro-4-(((1S,2S,4S)-4-(3-chlorophenyl)-2-(dimethylamino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide and 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-phenylcyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (217 mg, 69%). 5-Chloro-4-(((1S,2S,4S)-4-(3-chlorophenyl)-2-(dimethylamino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide is the major species. LCMS (ESI) m/z: 654.3 [M+H]+.
    • Step 5
  • Figure US20200115354A1-20200416-C00586
    • 5-Chloro-4-(((1S,2S,4S)-4-(3-chlorophenyl)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide Formate
  • To a mixture of 5-Chloro-4-(((1S,2S,4S)-4-(3-chlorophenyl)-2-(dimethylamino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide and 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-phenylcyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (217 mg, 0.32 mmol) was added formic acid (1 mL, 26.5 mmol). The reaction was stirred at room temperature for 3 hours then concentrated. The crude was dissolved in a mixture of DMF and DMSO, filtered and purified by prep-HPLC (CSH C18 column, 5 m, 30×75 mm at 40° C.; 45 mL/min, 25-45% MeCN/10 mM aqueous ammonium bicarbonate, pH=10). Appropriate fractions were combined and lyophilized. To the solid obtained was added MeCN (0.30 mL), water (0.60 mL) and formic acid (25 uL). The flask was frozen and lyophilized to give 5-chloro-4-(((1S,2S,4S)-4-(3-chlorophenyl)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide formate (21 mg, 14% yield). LCMS (ESI) m/z: 538.3 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.37 (s, 1H), 8.14 (s, 1H), 8.04 (d, J=5.1 Hz, 1H), 7.65 (d, J=7.3 Hz, 1H), 7.37 (d, J=2.0 Hz, 1H), 7.34 (d, J=7.7 Hz, 1H), 7.31-7.23 (m, 2H), 6.74 (d, J=12.8 Hz, 1H), 6.66 (d, J=5.7 Hz, 1H), 5.85 (s, 1H), 3.60 (bs, 1H), 3.13 (bs, 1H), 2.78-2.62 (m, 1H), 2.37 (s, J=15.0 Hz, 6H), 2.20-1.94 (m, 2H), 1.79-1.50 (m, 3H), 1.47-1.27 (m, 1H).
    • Example 232
  • Figure US20200115354A1-20200416-C00587
    • 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-phenylcyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 231 step 5, 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-phenylcyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide formate (43 mg) was obtained. LCMS (ESI) m/z: 504.3 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.38 (s, 1H), 8.14 (s, 1H), 8.05 (d, J=5.8 Hz, 1H), 7.65 (d, J=7.3 Hz, 1H), 7.39-7.27 (m, 4H), 7.21 (t, J=6.6 Hz, 1H), 6.79 (d, J=12.9 Hz, 1H), 6.68 (d, J=5.9 Hz, 1H), 5.89 (d, J=6.8 Hz, 1H), 3.66 (bs, 1H), 3.29-3.03 (m, 1H), 2.76-2.58 (m, 1H), 2.41 (s, 6H), 2.19-1.99 (m, 2H), 1.84-1.54 (m, 3H), 1.49-1.28 (m, 1H).
    • Example 233
  • Figure US20200115354A1-20200416-C00588
    • 5-Chloro-4-(((3S,4S)-3-(dimethylamino)-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00589
    Figure US20200115354A1-20200416-C00590
    • Step 1
  • Figure US20200115354A1-20200416-C00591
    • Rac-(3S,4S)-methyl 4-((tert-butoxycarbonyl)amino)-1-(3-(trifluoromethyl)phenyl)piperidine-3-carboxylate
  • 1-Bromo-3-(trifluoromethyl)benzene (0.25 ml, 1.81 mmol), racemic (3S,4S)-methyl 4-((tert-butoxycarbonyl)amino)piperidine-3-carboxylate, cesium carbonate (742 mg, 2.26 mmol), rac-BINAP (141 mg, 0.23 mmol) and tris(dibenzylideneacetone)-dipalladium(0) (138 mg, 0.15 mmol) were charged to a screw-cap reaction vial, which was flushed with nitrogen for 5 min. N2 degassed toluene (4 mL) was added and the mixture was bubbled with nitrogen for 5 minutes. The vial was capped and stirred at 110° C. for 16 hours. The reaction mixture was cooled to room temperature and purified directly by flash column chromatography through Si gel (EtOAc/hexanes) to provide rac-(3S,4S)-methyl 4-((tert-butoxycarbonyl)amino)-1-(3-(trifluoromethyl)phenyl)piperidine-3-carboxylate (320 mg, 53%) as a pale yellow solid. LCMS (ESI) m/z: 403.3 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 7.33 (t, J=7.9 Hz, 1H), 7.18-7.00 (m, 3H), 4.58 (s, 1H), 4.02-3.82 (m, 1H), 3.76 (ddd, J=12.9, 3.8, 2.1 Hz, 1H), 3.72 (s, 3H), 3.65 (ddd, J=12.7, 6.0, 4.0 Hz, 1H), 3.17 (dd, J=12.9, 10.4 Hz, 1H), 2.97 (ddd, J=12.8, 11.6, 2.9 Hz, 1H), 2.60 (td, J=10.2, 3.8 Hz, 1H), 2.26-2.07 (m, 1H), 1.62-1.54 (m, 1H), 1.43 (s, 9H).
    • Step 2
  • Figure US20200115354A1-20200416-C00592
    • Rac-(3S,4S)-4-((tert-butoxycarbonyl)amino)-1-(3-(trifluoromethyl)phenyl)piperidine-3-carboxylic Acid
  • Following the procedure described in Example 219 and making non-critical variations as required to replace (4aS,8aS)-tert-butyl 6-(3-bromophenyl)-4-oxo-2,4,4a,5,8,8a-hexahydro-1H-benzo[d][1,3]oxazine-1-carboxylate with rac-(3S,4S)-methyl 4-((tert-butoxycarbonyl)amino)-1-(3-(trifluoromethyl)phenyl)piperidine-3-carboxylate, rac-(3S,4S)-4-((tert-butoxycarbonyl)amino)-1-(3-(trifluoromethyl)phenyl)piperidine-3-carboxylic acid (310 mg, 100%) was obtained as an off-white solid. LCMS (ESI) m/z: 387.4 [M−H]−. 1H NMR (400 MHz, CDCl3 and methanol-d4 (9:1 v/v ratio)) δ 7.29 (t, J=8.0 Hz, 1H), 7.10-6.95 (m, 3H), 3.83 (s, 1H), 3.75 (dq, J=12.1, 1.3 Hz, 1H), 3.59 (d, J=12.9 Hz, 1H), 3.09 (dd, J=12.6, 10.6 Hz, 1H), 2.91 (td, J=12.8, 2.7 Hz, 1H), 2.52 (td, J=10.0, 3.3 Hz, 1H), 2.07 (d, J=9.9 Hz, 1H), 1.54 (q, J=9.3 Hz, 1H), 1.37 (s, 9H). 2 H exchanged with the solvent and are not observed.
    • Step 3
  • Figure US20200115354A1-20200416-C00593
    • Rac-tert-butyl((3S,4S)-3-isocyanato-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)carbamate
  • Following the procedure described in Example 219 and making non-critical variations as required to replace (3S,4S)-3′-bromo-4-((tert-butoxycarbonyl)amino)-2,3,4,5-tetrahydro-[1,1′-biphenyl]-3-carboxylic acid with rac-(3S,4S)-4-((tert-butoxycarbonyl)amino)-1-(3-(trifluoromethyl)phenyl)piperidine-3-carboxylic acid, rac-tert-butyl((3S,4S)-3-isocyanato-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)carbamate (290 mg, 58%) was obtained as a colorless oil. LCMS (ESI) m/z: 408.3 [M+Na]+.
    • Step 4
  • Figure US20200115354A1-20200416-C00594
    • Rac-tert-butyl((3S,4S)-3-amino-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)carbamate
  • Following the procedure described in Example 219 and making non-critical variations as required to replace tert-butyl((3S,4S)-3′-bromo-3-isocyanato-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate with rac-tert-butyl((3S,4S)-3-isocyanato-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)carbamate, rac-tert-butyl((3S,4S)-3-amino-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)carbamate (250 mg, 92%) was obtained as a colorless oil. LCMS (ESI) m/z: 304.2 [M+H-tBu]+.
    • Step 5
  • Figure US20200115354A1-20200416-C00595
    • Rac-tert-butyl((3S,4S)-3-(dimethylamino)-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)carbamate
  • Following the procedure described in Example 219 and making non-critical variations as required to replace tert-butyl((3S,4S)-3-amino-3′-bromo-2,3,4,5-tetrahydro-[1,1′-biphenyl]-4-yl)carbamate with rac-tert-butyl((3S,4S)-3-amino-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)carbamate, crude rac-tert-butyl((3S,4S)-3-(dimethylamino)-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)carbamate (282 mg, 100%) was obtained as a pale yellow oil. LCMS (ESI) m/z: 388.3 [M+H]+.
    • Step 6
  • Figure US20200115354A1-20200416-C00596
    • Rac-(3S,4S)—N3,N3-dimethyl-1-(3-(trifluoromethyl)phenyl)piperidine-3,4-diamine hydrochloride
  • Following the procedure described in Example 219 and making non-critical variations as required to replace tert-butyl((1S,2S,4S)-4-(3-cyclopropylphenyl)-2-(dimethylamino)cyclohexyl)carbamate with crude rac-tert-butyl((3S,4S)-3-(dimethylamino)-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)carbamate, rac-(3S,4S)—N3,N3-dimethyl-1-(3-(trifluoromethyl)phenyl)piperidine-3,4-diamine hydrochloride (307 mg, 100%) was obtained as an off-white solid. LCMS (ESI) m/z: 288.1 [M+H]+. 1H NMR (400 MHz, methanol-d4) δ 7.50 (t, J=7.8 Hz, 1H), 7.35 (d, J=8.0 Hz, 2H), 7.22 (d, J=7.5 Hz, 1H), 4.08-4.03 (m, 1H), 4.02-3.97 (m, 1H), 3.75 (td, J=8.2, 3.7 Hz, 1H), 3.63 (dd, J=11.4, 5.7 Hz, 1H), 3.47 (dd, J=13.4, 8.3 Hz, 1H), 3.23 (ddd, J=13.1, 9.8, 3.6 Hz, 1H), 3.06 (br s, 6H), 2.39 (ddd, J=13.8, 8.8, 5.0 Hz, 1H), 2.10-1.98 (m, 1H).
    • Step 7
  • Figure US20200115354A1-20200416-C00597
    • Rac-5-chloro-N-(2,4-dimethoxybenzyl)-4-(((3S,4S)-3-(dimethylamino)-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 219 and making non-critical variations as required to replace (1S,2S,5S)-5-(3-cyclopropylphenyl)-N,N-dimethylcyclohexane-1,2-diamine with crude rac-(3S,4S)—N3,N3-dimethyl-1-(3-(trifluoromethyl)phenyl)piperidine-3,4-diamine hydrochloride, rac-5-chloro-N-(2,4-dimethoxybenzyl)-4-(((3S,4S)-3-(dimethylamino)-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (351 mg, 62%) was obtained as a pale yellow oil. LCMS (ESI) m/z: 723.5 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 8.80 (d, J=0.8 Hz, 1H), 8.45 (d, J=5.9 Hz, 1H), 7.89 (d, J=7.2 Hz, 1H), 7.37 (t, J=7.9 Hz, 1H), 7.31 (dd, J=6.0, 1.2 Hz, 1H), 7.20 (d, J=8.2 Hz, 1H), 7.16-7.02 (m, 3H), 6.45-6.36 (m, 2H), 6.31 (d, J=12.5 Hz, 1H), 5.75 (d, J=2.5 Hz, 1H), 5.27 (s, 2H), 3.87 (d, J=10.2 Hz, 1H), 3.80 (s, 3H), 3.76 (s, 3H), 3.72 (d, J=13.1 Hz, 1H), 3.26 (tt, J=10.1, 3.3 Hz, 1H), 2.93-2.76 (m, 3H), 2.45-2.32 (m, 7H), 1.59 (qd, J=13.0, 4.1 Hz, 1H).
    • Step 8
  • Figure US20200115354A1-20200416-C00598
    • 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((3S,4S)-3-(dimethylamino)-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide & 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((3R,4R)-3-(dimethylamino)-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Rac-5-chloro-N-(2,4-dimethoxybenzyl)-4-(((3S,4S)-3-(dimethylamino)-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide was separated using chiral HPLC (Chiralpak IB, 250 mm×20 mm ID, 5 μm), 7:7:86 MeOH:DCM:hexane containing 0.1% of diethylamine, 15 ml/min) to afford: 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((3S,4S)-3-(dimethylamino)-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (peak 1, 145 mg, 37%) as a white solid, LCMS (ESI) m/z: 723.5 [M+H]+; and 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((3R,4R)-3-(dimethylamino)-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (peak 2, 150 mg, 38%) as a white solid, LCMS (ESI) m/z: 723.5 [M+H]+. Absolute configuration was arbitrarily assigned to each isomer.
    • Step 9
  • Figure US20200115354A1-20200416-C00599
    • 5-Chloro-4-(((3S,4S)-3-(dimethylamino)-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 219 and making non-critical variations as required to replace 5-chloro-4-(((1S,2S,4S)-4-(3-cyclopropylphenyl)-2-(dimethylamino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((3S,4S)-3-(dimethylamino)-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide, 5-chloro-4-(((3S,4S)-3-(dimethylamino)-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (84 mg, 73%) was obtained as a white solid. LCMS (ESI) m/z: 573.1 [M+H]+. 1H NMR (400 MHz, dmso-d6) δ 8.53 (s, 1H), 8.27-8.20 (m, 1H), 7.68 (d, J=7.4 Hz, 1H), 7.42 (t, J=8.0 Hz, 1H), 7.29-7.22 (m, 1H), 7.18 (s, 1H), 7.04 (d, J=7.6 Hz, 1H), 6.93-6.85 (m, 2H), 6.08-6.01 (m, 1H), 4.02-3.92 (m, 1H), 3.87-3.74 (m, 2H), 3.12-3.00 (m, 1H), 2.95 (t, J=11.9 Hz, 1H), 2.86 (t, J=11.9 Hz, 1H), 2.39 (s, 6H), 2.05-1.95 (m, 1H), 1.60-1.45 (m, 1H). Acidic NH hydrogen not observed. Absolute configuration was arbitrarily assigned.
    • Example 234
  • Figure US20200115354A1-20200416-C00600
    • 5-Chloro-4-(((3R,4R)-3-(dimethylamino)-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 219 and making non-critical variations as required to replace 5-chloro-4-(((1S,2S,4S)-4-(3-cyclopropylphenyl)-2-(dimethylamino)cyclohexyl)amino)-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide with 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((3R,4R)-3-(dimethylamino)-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide, 5-chloro-4-(((3R,4R)-3-(dimethylamino)-1-(3-(trifluoromethyl)phenyl)piperidin-4-yl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide formate (98 mg, 76%) was obtained as a white solid. LCMS (ESI) m/z: 573.0 [M+H]+. 1H NMR (400 MHz, dmso-d6) δ 8.48 (s, 1H), 8.17 (d, J=5.7 Hz, 1H), 8.14 (s, 1H), 7.66 (d, J=7.4 Hz, 1H), 7.42 (t, J=8.0 Hz, 1H), 7.28-7.22 (m, 1H), 7.17 (s, 1H), 7.03 (d, J=7.6 Hz, 1H), 6.84 (d, J=13.2 Hz, 1H), 6.81 (d, J=6.3 Hz, 1H), 5.95 (d, J=5.9 Hz, 1H), 3.97-3.89 (m, 1H), 3.85-3.71 (m, 2H), 3.02-2.90 (m, 2H), 2.84 (t, J=11.8 Hz, 1H), 2.36 (s, 6H), 2.06-1.96 (m, 1H), 1.57-1.44 (m, 1H). Acidic NH hydrogen not observed. Absolute configuration was arbitrarily assigned.
    • Example 235
  • Figure US20200115354A1-20200416-C00601
    • (1S,2S,5S)-2-((2-Chloro-5-fluoro-4-(N-(pyrimidin-4-yl)sulfamoyl)phenyl)amino)-N,N-dimethyl-5-(3-(trifluoromethyl)phenyl)cyclohexanamine Oxide
  • Figure US20200115354A1-20200416-C00602
    • Step 1
  • Figure US20200115354A1-20200416-C00603
    • (1S,2S,5S)-2-((2-Chloro-5-fluoro-4-(N-(pyrimidin-4-yl)sulfamoyl)phenyl)amino)-N,N-dimethyl-5-(3-(trifluoromethyl)phenyl)cyclohexanamine Oxide
  • To a stirred solution of 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S, 4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (29 mg, 0.05 mmol) in a mixture of chloroform (0.50 mL) and IPA (0.50 mL) was added 3-chloroperbenzoic acid (mCPBA) (12.5 mg, 0.06 mmol). The mixture was then stirred at 25° C. for 15 min then the solvent was removed in vacuo and formic acid (1.0 mL) was added. The mixture was stirred at rt for 3 hours then the solvent was removed in vacuo and purified directly by C18 reverse phase flash chromatography (MeCN/10 mM aqueous ammonium bicarbonate, pH=10). Appropriate fractions combined and lyophilized to provide (1S,2S,5S)-2-((2-chloro-5-fluoro-4-(N-(pyrimidin-4-yl)sulfamoyl)phenyl)amino)-NN-dimethyl-5-(3-(trifluoromethyl)phenyl)cyclohexanamine oxide (19 mg, 64% yield). LCMS (ESI) m/z: 588.1 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 8.44 (s, 1H), 8.12 (s, 2H), 7.72-7.55 (m, 5H), 6.77 (d, J=5.8 Hz, 1H), 6.67 (d, J=13.1 Hz, 1H), 3.85 (d, J=30.4 Hz, 2H), 3.16 (s, 3H), 3.12 (s, 3H), 2.92 (m 1H), 2.31 (m, 1H), 2.15 (m, 1H), 1.90-1.70 (m, 3H), 1.45 (s, 1H). Acidic NH hydrogen not observed.
    • Example 236
  • Figure US20200115354A1-20200416-C00604
    • 5-cyclobutyl-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00605
    • Step 1
  • Figure US20200115354A1-20200416-C00606
    • 5-cyclobutyl-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • To a (˜20 mL) borosilicate glass vial equipped with a Teflon-coated magnetic stir bar was added nickel glyme (2.87 mg, 0.010 mmol) and 4,4-Di-tert-butyl-2,2-dipyridyl (3.5 mg, 0.010 mmol) and THF (1 mL). The vial was capped and the resulting suspension was heated briefly with a heat gun until the nickel and ligand were fully solubilized yielding a pale green solution. The solvent was then removed under vacuum to give a fine coating of the ligated nickel complex (pale evergreen in color). Once dry, 5-bromo-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide prepared in Example 227 (100 mg, 0.13 mmol), Potassium cyclobutyltrifluoroborate (32 mg, 0.20 mmol), [Ir{dF(CF3)ppy}2(dtbpy)]PF6 (15 mg, 0.010 mmol) and Cesium carbonate (64 mg, 0.20 mmol) were added in that order. The vial was then capped and 1,4-Dioxane (5 mL) was introduced. The reaction was purged with N2 for 3 min and stirred ˜4 cm away from LED blue light. A fan was blown across the reaction setup to maintain an ambient temperature ˜24° C. The reaction was monitored by LCMS and stopped at 80 min. The reaction was then passed through a pad of silica, washed with 20% MeOH in CH2Cl2 and the filtrate was concentrated and dissolved in DMSO and purified by reverse phase prep-HPLC (75-100% MeCN/100 mM aqueous NH4CO3H, pH: 10 gradient over 10 min, XBridge BEH C18 OBD Prep Column, 130 Å, 5 μm, 30 mm×50 mm). Appropriate fractions combined and lyophilized to provide 5-cyclobutyl-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (31 mg, 32%). LCMS (ESI) m/z: 742.4 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00607
    • 5-cyclobutyl-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Following the procedure described in Example 187 and making non-critical variations as required to replace 5-chloro-N-(2,4-dimethoxybenzyl)-4-(((1S,2S)-2-(dimethylamino)-4,4-difluorocyclohexyl)amino)-2-fluoro-N-(thiazol-2-yl)benzenesulfonamide with 5-cyclobutyl-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (31 mg, 0.04 mmol), 5-cyclobutyl-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide was obtained (21 mg, 85%) as a white solid. LCMS (ESI) m/z: 592.3 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 8.51 (s, 1H), 8.23 (s, 1H), 7.68-7.50 (m, 5H), 7.44 (d, J=8.3 Hz, 1H), 6.87 (d, J=5.8 Hz, 1H), 6.50 (d, J=13.7 Hz, 1H), 5.33 (s, 1H), 2.93 (m, 1H), 2.77 (m, 1H), 2.34 m, 3H), 2.27 (s, 6H), 2.17 (m, 1H), 1.99 m, 3H), 1.91-1.69 (m, 5H), 1.59 (m, 1H), 1.27 (m, 1H).
    • Example 237
  • Figure US20200115354A1-20200416-C00608
    • 4-(((1S,2S,4S)-2-(Dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide Formate
  • Figure US20200115354A1-20200416-C00609
    • Step 1
  • Figure US20200115354A1-20200416-C00610
    • N-(2,4-Dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • To a solution of 5-bromo-N-(2,4-dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (20 mg, 0.03 mmol) and potassium cyclobutyl(trifluoro)boron (5.5 mg, 0.03 mmol), palladium(II) acetate (1.2 mg, 0.01 mmol) and cesium carbonate (26.0 mg, 0.08 mmol) in toluene (0.50 mL) and water (0.05 mL) was added n-butyl-di-1-adamantylphosphine (1.9 mg, 0.01 mmol) and the reaction was degasssed with N2 for 10 min. The reaction was heated to 100° C. overnight. The reaction was diluted with water (3 mL) and EtOAc (5 mL). The organic layer was separated, dried over Na2SO4, filtered and concentrated in vacuo. Purification by prep-HPLC (CSH column, 60-80% MeCN/10 mM aqueous ammonium bicarbonate pH=10) provided N-(2,4-dimethoxybenzyl)-4-(((1S,2S, 4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (9 mg, 50% yield). LCMS (ESI) m/z: 688.3 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00611
    • 4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 115 and making non-critical variations as required using N-(2,4-dimethoxybenzyl)-4-(((1S,2S, 4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (8 mg, 0.01 mmol), 4-(((1S,2S, 4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide (5 mg, 80% yield) was obtained. LCMS (ESI) m/z: 538.2 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 8.49 (s, 1H), 8.19 (s, 1H), 8.14 (s, 1H), 7.69-7.49 (m, 6H), 6.83 (s, 1H), 6.53-6.37 (m, 3H), 3.61-3.48 (m, 1H), 2.84-2.75 (m, 2H), 2.30 (s, 6H), 2.11 (dd, J=12.1, 2.4 Hz, 1H), 1.99 (d, J=10.8 Hz, 1H), 1.80-1.68 (m, 2H), 1.66-1.52 (m, 1H), 1.33-1.19 (m, 1H).
    • Example 238
  • Figure US20200115354A1-20200416-C00612
    • 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)oxy)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Figure US20200115354A1-20200416-C00613
    • Step 1:
  • Figure US20200115354A1-20200416-C00614
    • (1S,2S)-2-((tert-butyldimethylsilyl)oxy)cyclohexan-1-amine
  • Tert-butyldimethylchlorosilane (1.0 g, 6.56 mmol) was added to a solution of (1S,2S)-2-aminocyclohexanol (630 mg, 5.47 mmol) and triethylamine (0.91 mL, 6.56 mmol) in dichloromethane (10 mL) and the mixture was stirred at room temperature for 4h. Water was added and the phases were separated. The aqueous phase was extracted with dichloromethane. The organic phase was dried with MgSO4, filtered and concentrated to give the title compound (875 mg, 70%) as a yellow oil which was used for next step without purification.
    • Step 2:
  • Figure US20200115354A1-20200416-C00615
    • (1S,2S,4S)-2-amino-4-(3-(trifluoromethyl)phenyl)cyclohexan-1-ol
  • 2-hydroxynicotinaldehyde (27 mg, 0.22 mmol), palladium(II) acetate (24 mg, 0.11 mmol), 3-iodotrifluorotoluene (0.31 mL, 2.2 mmol), silver trifluoroacetate (481 mg, 2.2 mmol), water (0.20 mL), 1,1,1,3,3,3-hexafluoro-2-propanol (5.2 mL), acetic acid (0.27 mL) were combined in a sealable high pressure vial. (1R,2R)-2-[tert-butyl(dimethyl)silyl]oxycyclohexanamine (250 mg, 1.09 mmol) was added and the mixture was sealed and heated at 120° C. for 16h. The mixture was cooled down and filtered through celite. The filtrate was concentrated. The residue was dissolved in THF (5 mL) and 1N HCl (2 mL) was added. The mixture was stirred at room temperature for 1 h. The mixture was concentrated to remove THF and the organic phase was extracted with DCM. The aqueous phase was then basified by addition of 10N KOH. The aqueous phase was extracted with DCM. The organic phase was dried with MgSO4, filtered and concentrated to give the title compound (35 mg, 12.4%) as a yellow solid which was used for next step without purification.
    • Step 3:
  • Figure US20200115354A1-20200416-C00616
    • (1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexan-1-ol
  • Sodium triacetoxyborohydride (286.1 mg, 1.35 mmol) was added to a solution of (1S,2S,4S)-2-amino-4-[3-(trifluoromethyl)phenyl]cyclohexanol (35 mg, 0.13 mmol) and formaldehyde (37% in water) (0.06 mL, 0.81 mmol) in acetonitrile (3 mL) and the reaction was stirred at room temperature for 1h. The reaction was quenched by addition of aqueous NH4Cl. The mixture was concentrated to remove acetonitrile and the residue was extracted with DCM. The organic phase was dried with MgSO4, filtered and concentrated to give the title compound (21 mg, 54%) as a yellow solid which was used for next step without purification.
    • Step 4:
  • Figure US20200115354A1-20200416-C00617
    • 5-chloro-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)oxy)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide
  • Sodium hydride (60 mass %) in mineral oil (8.8 mg, 0.22 mmol) was added to a solution of (1S,2S,4S)-2-(dimethylamino)-4-[3-(trifluoromethyl)phenyl]cyclohexanol (21 mg, 0.07 mmol) in N,N-dimethylformamide (0.5 mL). The reaction was stirred for 10 min then 5-chloro-N-[(2,4-dimethoxyphenyl)methyl]-2,4-difluoro-N-pyrimidin-4-yl-benzenesulfonamide (33.32 mg, 0.07 mmol) was added and the mixture was stirred at room temperature for 3h. The reaction was quenched by slow addition of water (1 mL). The resulting precipitate was collected by filtration and washed with water. The crude solid was purified by silica gel chromatography (0 to 100% EtOAc/Hep). The purified product was then stirred in formic acid (1 mL) for 1 h. The mixture was then concentrated and dried under vacuum for 16h to give the title compound (3.9 mg, 13%) as a white solid. LCMS (ESI) m/z: 573.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.33 (s, 1H), 8.14 (s, 2H), 8.00 (d, J=6.1 Hz, 1H), 7.79 (d, J=7.4 Hz, 1H), 7.59 (d, J=13.3 Hz, 3H), 7.29 (d, J=11.7 Hz, 1H), 6.62 (d, J=6.1 Hz, 1H), 6.53 (d, J=15.5 Hz, 1H), 4.77 (td, J=10.2, 4.4 Hz, 1H), 2.41 (s, 6H), 2.24-2.07 (m, 1H), 2.03-1.94 (m, 1H), 1.79-1.61 (m, 2H), 1.31-1.12 (m, 2H), 0.91-0.83 (m, 1H).
    • Example 239
  • Figure US20200115354A1-20200416-C00618
    • 5-chloro-4-(((1S,2S,4S)-4-(3,5-dichlorophenyl)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide Formate
  • Following the sequence described in Example 224 and making non-critical variations as required to replace 1-(4-(trifluoromethyl)phenyl)ethanone with 1-(3,5-dichlorophenyl)ethanone, 5-chloro-4-(((1S,2S,4S)-4-(3,5-dichlorophenyl)-2-(dimethylamino)cyclohexyl)amino)-2-fluoro-N-(pyrimidin-4-yl)benzenesulfonamide formate was obtained as a white solid. LCMS (ESI) m/z: 572.1, 574.1 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 8.27 (s, 1H), 8.15 (s, 1H), 7.93 (d, J=3.9 Hz, 1H), 7.60 (d, J=7.2 Hz, 1H), 7.51-7.29 (m, 2H), 6.68-6.46 (m, 3H), 5.67 (s, 1H), 2.88-2.68 (m, 3H), 2.21 (s, 6H), 1.94 (d, J=11.8 Hz, 1H), 1.78-1.66 (m, 2H), 1.53 (q, J=11.8 Hz, 1H), 1.38-1.16 (m, 2H). Acidic NH hydrogen not observed.
    • Example 240
  • Figure US20200115354A1-20200416-C00619
    • 4-(((1S,2S,4S)-2-(Dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-5-hydroxy-N-(pyrimidin-4-yl)benzenesulfonamide Formate
  • Figure US20200115354A1-20200416-C00620
    • Step 1
  • Figure US20200115354A1-20200416-C00621
    • N-(2,4-Dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-5-hydroxy-N-(pyrimidin-4-yl)benzenesulfonamide
  • To an oven-dried 10 mL screw-cap test tube equipped with a Teflon-coated magnetic stir bar was charged 2-(di-t-butylphosphino)-3,6-dimethoxy-2′,4′,6′-tri-i-propyl-1,1′-biphenyl, t-butylBrettPhos (25 mg, 0.05 mmol) and 5-bromo-N-[(2,4-dimethoxyphenyl)methyl]-4-[[(1S,2S,4S)-2-(dimethylamino)-4-[3-(trifluoromethyl)phenyl]cyclohexyl]amino]-2-fluoro-N-pyrimidin-4-ylbenzenesulfonamide (200 mg, 0.26 mmol). A solution of CsOH (117 mg, 0.78 mmol) in water (47 mg, 2.61 mmol) was then added followed by addition of 1,4-dioxane (0.2 mL). The reaction vessel was evacuated and backfilled with N2 (this process was repeated a total of three times). To this was then added a solution of t-BuBrettPhos Pd G3 (44.6 mg, 0.05 mmol) in 1,4-Dioxane (1 mL) via syringe and the mixture stirred at rt for 3 h. The mixture was then diluted with EtOAc (25 mL) and then washed with saturated aqueous NH4Cl (10 mL). The organic layer was separated and washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. Purification by flash column chromatography through Si gel (0-20% MeOH/DCM) afforded N-[(2,4-dimethoxyphenyl)methyl]-4-[[(1S,2S,4S)-2-(dimethylamino)-4-[3-(trifluoromethyl)phenyl]cyclohexyl]amino]-2-fluoro-5-hydroxy-N-pyrimidin-4-yl-benzenesulfonamide (193 mg, 105%). LCMS (ESI) m/z: 704.3 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00622
    • 4-(((1S,2S,4S)-2-(Dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-5-hydroxy-N-(pyrimidin-4-yl)benzenesulfonamide formate
  • Following the procedure described in Example 115 step 2 and making non-critical variations as required using N-[(2,4-dimethoxyphenyl)methyl]-4-[[(1S,2S,4S)-2-(dimethylamino)-4-[3-(trifluoromethyl)phenyl]cyclohexyl]amino]-2-fluoro-5-hydroxy-N-pyrimidin-4-yl-benzenesulfonamide (193 mg, 0.26 mmol), 4-(((1S,2S,4S)-2-(Dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-5-hydroxy-N-(pyrimidin-4-yl)benzenesulfonamide formate (66 mg, 84%) was obtained. LCMS (ESI) m/z: 554.2 [M+H]+. 1H NMR (400 MHz, d6-DMSO) δ 8.45 (s, 1H), 8.13 (s, 1H), 7.64-7.53 (m, 4H), 7.11 (d, J=7.0 Hz, 1H), 6.78 (d, J=5.9 Hz, 1H), 6.46 (d, J=12.8 Hz, 1H), 5.51 (s, 1H), 2.97 (m, 1H), 2.78 (m, 1H), 2.29 (s, 6H), 2.16 (m, 1H), 2.00 (m, 1H), 1.72 (m, 2H), 1.58 (m, 1H), 1.27 (m, 1H).
    • Example 241
  • Figure US20200115354A1-20200416-C00623
    • 4-(((1S,2S,4S)-2-(Dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-5-methoxy-N-(pyrimidin-4-yl)benzenesulfonamide Formate
  • Figure US20200115354A1-20200416-C00624
    • Step 1
  • Figure US20200115354A1-20200416-C00625
    • N-(2,4-Dimethoxybenzyl)-4-(((1S,2S,4S)-2-(dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-5-methoxy-N-(pyrimidin-4-yl)benzenesulfonamide
  • A reaction flask was charged with THF (0.40 mL), triphenylphosphine (63 mg, 0.24 mmol) and diisopropyl azodicarboxylate (0.05 mL, 0.24 mmol) in that order. The mixture was stirred at rt for 5 min before addition of a 5% solution of MeOH in THF (0.17 mL, 0.24 mmol). This mixture was stirred for another 5 min at rt before addition of N-[(2,4-dimethoxyphenyl)methyl]-4-[[(1S,2S,4S)-2-(dimethylamino)-4-[3-(trifluoromethyl)phenyl]cyclohexyl]amino]-2-fluoro-5-hydroxy-N-pyrimidin-4-yl-benzenesulfonamide (100 mg, 0.14 mmol). The reaction was stirred at rt for 1 h, concentrated and dissolved in DMSO (3 mL) and purified directly by Prep-HPLC (CSH column, 65-85% MeCN/10 mM aqueous ammonium bicarbonate, pH=10). Appropriate fractions combined and lyophilized to provide N-[(2,4-dimethoxyphenyl)methyl]-4-[[(1S,2S,4S)-2-(dimethylamino)-4-[3-(trifluoromethyl)phenyl]cyclohexyl]amino]-2-fluoro-5-methoxy-N-pyrimidin-4-yl-benzene sulfonamide (37 mg, 36%). LCMS (ESI) m/z: 718.3 [M+H]+.
    • Step 2
  • Figure US20200115354A1-20200416-C00626
    • 4-(((1S,2S,4S)-2-(Dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-5-methoxy-N-(pyrimidin-4-yl)benzenesulfonamide Formate
  • Following the procedure described in Example 115 step 2 and making non-critical variations as required using N-[(2,4-dimethoxyphenyl)methyl]-4-[[(1S,2S,4S)-2-(dimethylamino)-4-[3-(trifluoromethyl)phenyl]cyclohexyl]amino]-2-fluoro-5-methoxy-N-pyrimidin-4-yl-benzenesulfonamide (37 mg, 0.052 mmol), 4-(((1S,2S,4S)-2-(Dimethylamino)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)-2-fluoro-5-methoxy-N-(pyrimidin-4-yl)benzenesulfonamide formate (27 mg, 92%). LCMS (ESI) m/z: 568.2 [M+H]+. H NMR (400 MHz, d6-DMSO) δ 8.46 (s, 1H), 8.18-8.09 (m, 2H), 7.71-7.49 (m, 5H), 7.15 (d, J=6.5 Hz, 1H), 6.81 (d, J=5.8 Hz, 1H), 6.54 (d, J=12.6 Hz, 1H), 5.72 (d, J=6.8 Hz, 1H), 3.78 (s, 3H), 3.59-3.49 (m, 1H), 3.12-2.99 (m, 1H), 2.86-2.73 (m, 1H), 2.34 (s, 6H), 2.15 (dd, J=12.3, 4.1 Hz, 1H), 2.03 (d, J=12.2 Hz, 1H), 1.79-1.70 (m, 2H), 1.62 (q, J=12.2 Hz, 1H), 1.38-1.24 (m, 1H).
    • Example 242
  • Figure US20200115354A1-20200416-C00627
    • 5-chloro-2-fluoro-N-(pyrimidin-4-yl)-4-(((1S,2S,4S)-2-(pyrrolidin-1-yl)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)benzenesulfonamide Step 1
  • Figure US20200115354A1-20200416-C00628
    • tert-butyl((1S,2S,4S)-2-(pyrrolidin-1-yl)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)carbamate
  • Following the procedure described in Example 92, step 2 and making non-critical variations as required to replace 4-(((1S,2S)-2-aminocyclohexyl)amino)-5-chloro-N-(2,4-dimethoxybenzyl)-2-fluoro-N-(4-methylthiazol-2-yl)benzenesulfonamide with tert-butyl((1S,2S,4S)-2-amino-4-(3-(trifluoromethyl)phenyl)cyclohexyl)carbamate, tert-butyl((1S,2S,4S)-2-(pyrrolidin-1-yl)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)carbamate was obtained as a yellow solid. 1H NMR (400 MHz, CDCl3) δ 7.48-7.35 (m, 4H), 5.41-5.35 (m, 1H), 3.38-3.25 (m, 1H), 2.73-2.52 (m, 7H), 2.18-2.01 (m, 1H), 1.75-1.72 (m, 1H), 1.69-1.57 (m, 4H), 1.56-1.51 (m, 2H), 1.47 (s, 9H), 1.48-1.26 (m, 1H).
    • Step 2
  • Figure US20200115354A1-20200416-C00629
    • 5-chloro-2-fluoro-N-(pyrimidin-4-yl)-4-(((1S,2S,4S)-2-(pyrrolidin-1-yl)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)benzenesulfonamide
  • Following the procedure described in Example 23 and making non-critical variations as required to replace tert-butyl((1S,6S)-6-(dimethylamino)-4-(trifluoromethyl)cyclohex-3-en-1-yl)carbamate with tert-butyl((1S,2S,4S)-2-(pyrrolidin-1-yl)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)carbamate, 5-chloro-2-fluoro-N-(pyrimidin-4-yl)-4-(((1S,2S,4S)-2-(pyrrolidin-1-yl)-4-(3-(trifluoromethyl)phenyl)cyclohexyl)amino)benzene-sulfonamide was obtained as a white solid. LCMS (ESI) m/z: 598.2 [M+H]+. 1H NMR (400 MHz, DMSO-d6) δ 8.35 (s, 1H), 8.06-8.01 (m, 1H), 7.67-7.57 (m, 3H), 7.56-7.54 (m, 2H), 6.80-6.71 (m, 1H), 6.66 (d, J=6.0 Hz, 1H), 5.97 (d, J=8.0 Hz, 1H), 3.98-3.46 (m, 1H), 3.15-3.10 (m, 3H), 2.98-2.89 (m, 2H), 2.84-2.80 (m, 1H), 2.16-2.04 (m, 2H), 1.91-1.69 (m, 7H), 1.49-1.41 (m, 1H). Exchangable NH hydrogens not observed.
  • Although the preparation of the free-base or a specific salt form may be illustrated in the Examples above, it is understood that the free-base and its acid or base salt forms can be interconverted using standard techniques
    • Example 243A. Tritiated Compound Binding to Membranes Isolated from Cells that Heterologously Express hNav1.7 and the β1 Subunit
  • Preparation of membranes containing recombinantly expressed sodium channels: Frozen recombinant cell pellets were thawed on ice and diluted to 4 times the cell pellet weight with ice cold 50 mM Tris HCl, pH 7.4 buffer. The cell suspensions were homogenized on ice using a motorized glass dounce homogeniser. Homogenates were further diluted 8.4 times with ice cold 50 mM Tris HCl, pH 7.4 buffer and then centrifuged at 200×g at 4° C. for 15 min. The supernatants were collected and centrifuged at 10000×g at 4° C. for 50 min. The pellets were then re-suspended in 100 mM NaCl, 20 mM Tris HCl, pH 7.4 buffer containing 1% v/v protease inhibitors (Calbiochem) and re-homogenized on ice. The homogenized membranes were then processed through a syringe equipped with a 26 gauge needle. Protein concentrations were determined by Bradford Assay and the membranes were stored at −80° C.
  • Radioligand Binding Studies:
  • Saturation experiments. A competitive NaV1.7 inhibitor having a methyl group was tritiated. Three tritiums were incorporated in place of methyl hydrogens to generate [3H]compound. Binding of this radioligand was performed in 5 mL borosilicate glass test tubes at room temperature. Binding was initiated by adding membranes to increasing concentrations of [3H]compound in 100 mM NaCl, 20 mM Tris HCl, pH 7.4 buffer containing 0.01% w/v bovine serum albumin (BSA) for 18h. Non-specific binding was determined in the presence of 1 μM unlabeled compound. After 18h, the reactants were filtered through GF/C glass fiber filters presoaked in 0.5% w/v polyethylene imine. Filters were washed with 15 mL ice cold 100 mM NaCl, 20 mM Tris HCl, pH7.4 buffer containing 0.25% BSA to separate bound from free ligand. [3H]compound bound to filters was quantified by liquid scintillation counting.
  • Competitive Binding Experiments:
  • Binding reactions were performed in 96-well polypropylene plates at room temperature for 18h. In 360 μL, membranes were incubated with 100 pM [3H]compound and increasing concentrations of Test Compound. Non-specific binding was defined in the presence of 1 μM unlabeled compound. Reactions were transferred and filtered through 96-well glass fiber/C filter plates presoaked with 0.5% polyethylene imine. The filtered reactions were washed 5 times with 200 μL ice cold buffer containing 0.25% BSA. Bound radioactivity was determined by liquid scintillation counting.
  • Data Analysis: For saturation experiments, non-specific binding was subtracted from total binding to provide specific binding and these values were recalculated in terms of pmol ligand bound per mg protein. Saturation curves were constructed and dissociation constants were calculated using the single site ligand binding model: Beq=(Bmax*X)/(X+Kd), where Beq is the amount of ligand bound at equilibrium, Bmax is the maximum receptor density, Kd is the dissociation constant for the ligand, and X is the free ligand concentration. For competition studies percent inhibition was determined and IC50 values were calculated using a 4 parameter logistic model (% inhibition=(A+((B−A)/(1+((x/C){circumflex over ( )}D)))) using XLfit, where A and B are the maximal and minimum inhibition respectively, C is the IC50 concentration and D is the (Hill) slope.
    • Example 243B. Tritiated Compound Binding to Membranes Isolated from Cells that Heterologous Express hNav1.7 and the β1 Subunit Preparation of Membranes Containing Recombinantly Expressed Sodium Channels:
  • Following the procedure described in Example 243A, and making modifications as required to: centrifuge supernatants at 100000×g instead of 10000× g; re-suspend pellets in a buffer containing 0.01% BSA in addition to 100 mM NaCl, 20 mM Tris HCl, pH 7.4 buffer, and 1% v/v proteasome inhibitors; and storing membranes at −80° C. in single use aliquots instead of pooled.
    • Radioligand Binding Studies: Saturation Experiments:
  • Following the procedure described in Example 243A, and making modifications as required to incubate [3H]compound for 3 hours instead of 18 hours.
    • Competitive Binding Experiments:
  • Following the procedure described in Example 243A, and making modifications as required to: perform binding experiments at room temperature for 3 hours instead of 18 hours; use 240 uL of solution and 300 pM of [3H]compound instead of 360 uL and 100 pM of [3H]compound; and wash filtered reactions 2 times instead of 5 times.
  • Data Analysis:
  • Following the procedure described in Example 243A.
    • Example 243C. Electrophysiological Assay (EP) (In Vitro Assay)
  • Patch voltage clamp electrophysiology allows for the direct measurement and quantification of block of voltage-gated sodium channels (NaV's), and allows the determination of the time- and voltage-dependence of block which has been interpreted as differential binding to the resting, open, and inactivated states of the sodium channel (Hille, B., Journal of General Physiology (1977), 69: 497-515).
  • The following voltage clamp electrophysiology studies were performed on representative compounds using cells heterologously expressing Nav1.7 or Nav1.5 channels. cDNAs for Nav1.7 (NM_002977) and Nav1.5 (AC137587) were stably expressed in Chinese Hamstr Ovary (CHO) cells and CHL (Chinese Hamster Lung) cells respectively. Sodium currents were measured in the whole-cell configuration using Syncropatch 384PE (Nanlon Technologies, Germany). 1NPC®-384 chips with custom medium resistance and single hole mode are used. Internal solution consists of (in mM): 110 CsCl, 10 CsCl, 20 EGTA, and 10 Hepes (pH adjusted to 7.2); and external solution contains (in mM): 60 NMDG, 80 NaCl, 4 KCl, 1 MgCl2, 2 CaCl2, 2 D-Glucose monohydrate, 10 Hepes (pH adjusted to 7.4 with NaOH).
  • After system flushing, testing compounds are dissolved in external solution containing 0.1% Pluronic F-127. The chip is moved into the measuring head and the instrument primes the chip with external and internal solutions. 10l cells are added to the chip from a cell hotel, and a negative pressure of −50 mBar is applied to form a seal. Following treatment with seal enhancer solution and wash-off with external solution, negative pressure of −250 mbar is applied for 1 second to achieve the whole-cell configuration, followed by three washing steps in external solution. 20 μl of compounds is added to 40 μl in each well (1:3 dilution of compounds), and after mixing, 20 μl is removed so the volume is retained at 40 ul. After approximately 13 minutes recordings, 20 μl/well of 2 uM TTX, or 333 uM Tetracaine (for Nav1.5) is added to achieve full block.
  • For voltage protocol, an holding potential of −50 mV is applied during the whole experiment. A depolarizing step is applied to −10 mV for 10 ms, followed by a hyperpolarization step to −150 mV for 20 ms to allow channel recovery from inactivation. A second depolarizing step is applied from −150 mV to −10 mV for 10 ms, where currents were measured to derive blocking effects of compounds. Inhibition is determined based on 7.5 min of compound incubation.
  • Data for representative compounds is provided in Table 1 and Table 2.
  • TABLE 1
    Nav1.7
    (LBA)
    Ex- (Ex.
    am- 243A)
    ple Structure (μM)
     1
    Figure US20200115354A1-20200416-C00630
         		0.197 
     2
    Figure US20200115354A1-20200416-C00631
         		0.0382 
     3
    Figure US20200115354A1-20200416-C00632
         		0.016 
     4
    Figure US20200115354A1-20200416-C00633
         		0.00415
     5
    Figure US20200115354A1-20200416-C00634
         		0.426 
     6
    Figure US20200115354A1-20200416-C00635
         		0.994 
     7
    Figure US20200115354A1-20200416-C00636
         		0.0899 
     8
    Figure US20200115354A1-20200416-C00637
         		3.00  
     9
    Figure US20200115354A1-20200416-C00638
         		3.00  
    10
    Figure US20200115354A1-20200416-C00639
         		0.553 
    11
    Figure US20200115354A1-20200416-C00640
         		0.126 
    12
    Figure US20200115354A1-20200416-C00641
         		0.165 
    13
    Figure US20200115354A1-20200416-C00642
         		0.357 
    14
    Figure US20200115354A1-20200416-C00643
         		0.0219 
    15
    Figure US20200115354A1-20200416-C00644
         		0.0269 
    16
    Figure US20200115354A1-20200416-C00645
         		0.325 
    17
    Figure US20200115354A1-20200416-C00646
         		0.237 
    18
    Figure US20200115354A1-20200416-C00647
         		0.0168 
    19
    Figure US20200115354A1-20200416-C00648
         		0.0451 
    20
    Figure US20200115354A1-20200416-C00649
         		0.211 
    21
    Figure US20200115354A1-20200416-C00650
         		0.214 
    22
    Figure US20200115354A1-20200416-C00651
         		0.0951 
    23
    Figure US20200115354A1-20200416-C00652
         		0.0084 
    24
    Figure US20200115354A1-20200416-C00653
         		0.0499 
    25
    Figure US20200115354A1-20200416-C00654
         		7.42  
    26
    Figure US20200115354A1-20200416-C00655
         		0.0415 
    27
    Figure US20200115354A1-20200416-C00656
         		0.149 
  • TABLE 2
    Nav1.7 Nav1.7 EP_SP
    LBA LBA hNav1.7
    (Ex. 243A) (Ex. 243B) (Ex. 243B)
    Example Structure IC50 (uM) IC50 (uM) IC50 (uM)
     28
    Figure US20200115354A1-20200416-C00657
         		0.199  
     29
    Figure US20200115354A1-20200416-C00658
         		0.0601 
     30
    Figure US20200115354A1-20200416-C00659
         		0.00973  0.00977 
     31
    Figure US20200115354A1-20200416-C00660
         		0.163  
     32
    Figure US20200115354A1-20200416-C00661
         		0.0382  0.0246  
     33
    Figure US20200115354A1-20200416-C00662
         		0.0824 
     34
    Figure US20200115354A1-20200416-C00663
         		0.0352 
     35
    Figure US20200115354A1-20200416-C00664
         		0.0655  0.313   0.0599  
     36
    Figure US20200115354A1-20200416-C00665
         		0.0936 
     37
    Figure US20200115354A1-20200416-C00666
         		0.00276 
     38
    Figure US20200115354A1-20200416-C00667
         		0.00505  0.0349  0.00306 
     39
    Figure US20200115354A1-20200416-C00668
         		0.00188  0.0128  0.0014  
     40
    Figure US20200115354A1-20200416-C00669
         		0.88  
     41
    Figure US20200115354A1-20200416-C00670
         		0.00451  0.0034  
     42
    Figure US20200115354A1-20200416-C00671
         		0.0272 
     43
    Figure US20200115354A1-20200416-C00672
         		1.13  
     44
    Figure US20200115354A1-20200416-C00673
         		0.0744 
     45
    Figure US20200115354A1-20200416-C00674
         		0.0127 
     46
    Figure US20200115354A1-20200416-C00675
         		0.77  
     47
    Figure US20200115354A1-20200416-C00676
         		0.529  
     48
    Figure US20200115354A1-20200416-C00677
         		0.127  
     49
    Figure US20200115354A1-20200416-C00678
         		0.0421 
     50
    Figure US20200115354A1-20200416-C00679
         		0.0584 
     51
    Figure US20200115354A1-20200416-C00680
         		0.0756 
     52
    Figure US20200115354A1-20200416-C00681
         		0.00848 
     53
    Figure US20200115354A1-20200416-C00682
         		0.0305  0.00569 
     54
    Figure US20200115354A1-20200416-C00683
         		0.0979 
     55
    Figure US20200115354A1-20200416-C00684
         		0.00738 
     56
    Figure US20200115354A1-20200416-C00685
         		0.00346 
     57
    Figure US20200115354A1-20200416-C00686
         		0.00242 
     58
    Figure US20200115354A1-20200416-C00687
         		0.00342 
     59
    Figure US20200115354A1-20200416-C00688
         		0.00194 
     60
    Figure US20200115354A1-20200416-C00689
         		0.198  
     61
    Figure US20200115354A1-20200416-C00690
         		0.558  
     62
    Figure US20200115354A1-20200416-C00691
         		0.0578 
     63
    Figure US20200115354A1-20200416-C00692
         		0.00981  0.00535 
     64
    Figure US20200115354A1-20200416-C00693
         		0.0116 
     65
    Figure US20200115354A1-20200416-C00694
         		0.0293 
     66
    Figure US20200115354A1-20200416-C00695
         		0.00207  0.00774  0.00291 
     67
    Figure US20200115354A1-20200416-C00696
         		0.0173 
     68
    Figure US20200115354A1-20200416-C00697
         		0.0166 
     69
    Figure US20200115354A1-20200416-C00698
         		0.296  
     70
    Figure US20200115354A1-20200416-C00699
         		0.00346 
     71
    Figure US20200115354A1-20200416-C00700
         		0.0413 
     72
    Figure US20200115354A1-20200416-C00701
         		0.0148 
     73
    Figure US20200115354A1-20200416-C00702
         		0.0197 
     74
    Figure US20200115354A1-20200416-C00703
         		0.0917 
     75
    Figure US20200115354A1-20200416-C00704
         		0.0379 
     76
    Figure US20200115354A1-20200416-C00705
         		0.116  
     77
    Figure US20200115354A1-20200416-C00706
         		0.0432 
     78
    Figure US20200115354A1-20200416-C00707
         		0.0956 
     79
    Figure US20200115354A1-20200416-C00708
         		0.00928 
     80
    Figure US20200115354A1-20200416-C00709
         		0.122  
     81
    Figure US20200115354A1-20200416-C00710
         		1.74  
     82
    Figure US20200115354A1-20200416-C00711
         		0.00335 
     83
    Figure US20200115354A1-20200416-C00712
         		0.379  
     84
    Figure US20200115354A1-20200416-C00713
         		3.0   
     85
    Figure US20200115354A1-20200416-C00714
         		0.252  
     86
    Figure US20200115354A1-20200416-C00715
         		0.0381 
     87
    Figure US20200115354A1-20200416-C00716
         		0.000539
     88
    Figure US20200115354A1-20200416-C00717
         		0.201  
     89
    Figure US20200115354A1-20200416-C00718
         		0.0599 
     90
    Figure US20200115354A1-20200416-C00719
         		0.00264 
     91
    Figure US20200115354A1-20200416-C00720
         		0.013  
     92
    Figure US20200115354A1-20200416-C00721
         		0.118  
     93
    Figure US20200115354A1-20200416-C00722
         		0.0647 
     94
    Figure US20200115354A1-20200416-C00723
         		0.0313 
     95
    Figure US20200115354A1-20200416-C00724
         		0.0243 
     96
    Figure US20200115354A1-20200416-C00725
         		0.63  
     97
    Figure US20200115354A1-20200416-C00726
         		0.0364 
     98
    Figure US20200115354A1-20200416-C00727
         		3.0   
     99
    Figure US20200115354A1-20200416-C00728
         		0.0655 
    100
    Figure US20200115354A1-20200416-C00729
         		0.127  
    101
    Figure US20200115354A1-20200416-C00730
         		0.0255 
    102
    Figure US20200115354A1-20200416-C00731
         		0.424   0.0665  
    103
    Figure US20200115354A1-20200416-C00732
         		0.0322 
    104
    Figure US20200115354A1-20200416-C00733
         		0.0356 
    105
    Figure US20200115354A1-20200416-C00734
         		0.0313  0.0045  
    106
    Figure US20200115354A1-20200416-C00735
         		0.67   0.0749  
    107
    Figure US20200115354A1-20200416-C00736
         		0.137   0.018  
    108
    Figure US20200115354A1-20200416-C00737
         		0.166   0.0226  
    109
    Figure US20200115354A1-20200416-C00738
         		0.0507  0.00868 
    110
    Figure US20200115354A1-20200416-C00739
         		0.0194  0.00371 
    111
    Figure US20200115354A1-20200416-C00740
         		0.678   0.0234  
    112
    Figure US20200115354A1-20200416-C00741
         		0.229   0.0151  
    113
    Figure US20200115354A1-20200416-C00742
         		0.185   0.0117  
    114
    Figure US20200115354A1-20200416-C00743
         		0.264   0.0331  
    115
    Figure US20200115354A1-20200416-C00744
         		0.018   0.00329 
    116
    Figure US20200115354A1-20200416-C00745
         		0.307   0.0182  
    117
    Figure US20200115354A1-20200416-C00746
         		2.22   0.97   
    118
    Figure US20200115354A1-20200416-C00747
         		0.242   0.00851 
    119
    Figure US20200115354A1-20200416-C00748
         		0.184   0.0107  
    120
    Figure US20200115354A1-20200416-C00749
         		0.0263  0.0041  
    121
    Figure US20200115354A1-20200416-C00750
         		1.17   0.121  
    122
    Figure US20200115354A1-20200416-C00751
         		0.205   0.0336  
    123
    Figure US20200115354A1-20200416-C00752
         		0.0175  0.00454 
    124
    Figure US20200115354A1-20200416-C00753
         		1.23   0.107  
    125
    Figure US20200115354A1-20200416-C00754
         		0.204   0.00753 
    126
    Figure US20200115354A1-20200416-C00755
         		0.0876  0.00925 
    127
    Figure US20200115354A1-20200416-C00756
         		0.0615  0.00658 
    128
    Figure US20200115354A1-20200416-C00757
         		0.0228  0.00956 
    129
    Figure US20200115354A1-20200416-C00758
         		0.000255 0.000372 
    130
    Figure US20200115354A1-20200416-C00759
         		0.0759  0.00992 
    131
    Figure US20200115354A1-20200416-C00760
         		0.201   0.00595 
    132
    Figure US20200115354A1-20200416-C00761
         		0.306   0.014  
    133
    Figure US20200115354A1-20200416-C00762
         		0.0208  0.0032  
    134
    Figure US20200115354A1-20200416-C00763
         		0.279   0.0186  
    135
    Figure US20200115354A1-20200416-C00764
         		0.313   0.0328  
    136
    Figure US20200115354A1-20200416-C00765
         		0.493   0.0301  
    137
    Figure US20200115354A1-20200416-C00766
         		0.00357  0.00124 
    138
    Figure US20200115354A1-20200416-C00767
         		0.00943  0.00321 
    139
    Figure US20200115354A1-20200416-C00768
         		0.291   0.0456  
    140
    Figure US20200115354A1-20200416-C00769
         		0.17   0.0132  
    141
    Figure US20200115354A1-20200416-C00770
         		0.275   0.0127  
    142
    Figure US20200115354A1-20200416-C00771
         		2.0    1.12   
    143
    Figure US20200115354A1-20200416-C00772
         		0.418   0.0193  
    144
    Figure US20200115354A1-20200416-C00773
         		0.374   0.0258  
    145
    Figure US20200115354A1-20200416-C00774
         		2.0    0.395  
    146
    Figure US20200115354A1-20200416-C00775
         		0.154   0.0154  
    147
    Figure US20200115354A1-20200416-C00776
         		0.275   0.0272  
    148
    Figure US20200115354A1-20200416-C00777
         		0.305   0.0253  
    149
    Figure US20200115354A1-20200416-C00778
         		0.52   0.0125  
    150
    Figure US20200115354A1-20200416-C00779
         		1.41   0.103  
    151
    Figure US20200115354A1-20200416-C00780
         		0.181   0.0201  
    152
    Figure US20200115354A1-20200416-C00781
         		2.0    1.68   
    153
    Figure US20200115354A1-20200416-C00782
         		0.0171  0.00407 
    154
    Figure US20200115354A1-20200416-C00783
         		1.2    0.101  
    155
    Figure US20200115354A1-20200416-C00784
         		0.13   0.0123  
    156
    Figure US20200115354A1-20200416-C00785
         		0.316   0.0189  
    157
    Figure US20200115354A1-20200416-C00786
         		0.337   0.0241  
    158
    Figure US20200115354A1-20200416-C00787
         		0.00596  0.00271 
    159
    Figure US20200115354A1-20200416-C00788
         		1.79   0.202  
    160
    Figure US20200115354A1-20200416-C00789
         		0.0203  0.00227 
    161
    Figure US20200115354A1-20200416-C00790
         		0.187   0.00303 
    162
    Figure US20200115354A1-20200416-C00791
         		0.248   0.00855 
    163
    Figure US20200115354A1-20200416-C00792
         		0.201   0.0181  
    164
    Figure US20200115354A1-20200416-C00793
         		0.0534  0.00743 
    165
    Figure US20200115354A1-20200416-C00794
         		2.0    0.511  
    166
    Figure US20200115354A1-20200416-C00795
         		0.341   0.0139  
    167
    Figure US20200115354A1-20200416-C00796
         		0.0917  0.0187  
    168
    Figure US20200115354A1-20200416-C00797
         		0.04   0.00516 
    169
    Figure US20200115354A1-20200416-C00798
         		0.00756  0.00334 
    170
    Figure US20200115354A1-20200416-C00799
         		0.00292  0.00368 
    171
    Figure US20200115354A1-20200416-C00800
         		0.255   0.0122  
    172
    Figure US20200115354A1-20200416-C00801
         		0.0382  0.0123  
    173
    Figure US20200115354A1-20200416-C00802
         		0.0177  0.0026  
    174
    Figure US20200115354A1-20200416-C00803
         		0.357   0.0502  
    175
    Figure US20200115354A1-20200416-C00804
         		0.316   0.0217  
    176
    Figure US20200115354A1-20200416-C00805
         		0.491   0.00872 
    177
    Figure US20200115354A1-20200416-C00806
         		0.0609  0.00291 
    178
    Figure US20200115354A1-20200416-C00807
         		0.205   0.0191  
    179
    Figure US20200115354A1-20200416-C00808
         		0.27   0.00683 
    180
    Figure US20200115354A1-20200416-C00809
         		0.666   0.0495  
    181
    Figure US20200115354A1-20200416-C00810
         		0.00131  0.000783 
    182
    Figure US20200115354A1-20200416-C00811
         		2.0    1.52   
    183
    Figure US20200115354A1-20200416-C00812
         		0.277   0.012  
    184
    Figure US20200115354A1-20200416-C00813
         		2.0    4.96   
    185
    Figure US20200115354A1-20200416-C00814
         		0.771   0.0362  
    186
    Figure US20200115354A1-20200416-C00815
         		0.0314  0.00408 
    187
    Figure US20200115354A1-20200416-C00816
         		0.776   0.00765 
    188
    Figure US20200115354A1-20200416-C00817
         		2.0    0.684  
    189
    Figure US20200115354A1-20200416-C00818
         		0.304   0.0234  
    190
    Figure US20200115354A1-20200416-C00819
         		0.478   0.0415  
    191
    Figure US20200115354A1-20200416-C00820
         		0.777   0.0394  
    192
    Figure US20200115354A1-20200416-C00821
         		0.324   0.0255  
    193
    Figure US20200115354A1-20200416-C00822
         		0.911   0.0394  
    194
    Figure US20200115354A1-20200416-C00823
         		2.0    0.4   
    195
    Figure US20200115354A1-20200416-C00824
         		0.00811  0.0014  
    196
    Figure US20200115354A1-20200416-C00825
         		0.664   0.12   
    197
    Figure US20200115354A1-20200416-C00826
         		0.038   0.00385 
    198
    Figure US20200115354A1-20200416-C00827
         		0.817   0.0658  
    199
    Figure US20200115354A1-20200416-C00828
         		0.131   0.0187  
    200
    Figure US20200115354A1-20200416-C00829
         		0.15   0.0167  
    201
    Figure US20200115354A1-20200416-C00830
         		0.308   0.0296  
    202
    Figure US20200115354A1-20200416-C00831
         		0.245   0.0344  
    203
    Figure US20200115354A1-20200416-C00832
         		0.0806  0.0116  
    204
    Figure US20200115354A1-20200416-C00833
         		0.308   0.0205  
    205
    Figure US20200115354A1-20200416-C00834
         		0.556   0.0327  
    206
    Figure US20200115354A1-20200416-C00835
         		0.0411  0.00865 
    207
    Figure US20200115354A1-20200416-C00836
         		1.6    0.203  
    208
    Figure US20200115354A1-20200416-C00837
         		0.0467  0.00612 
    209
    Figure US20200115354A1-20200416-C00838
         		0.16   0.00925 
    210
    Figure US20200115354A1-20200416-C00839
         		2.0    0.415  
    211
    Figure US20200115354A1-20200416-C00840
         		1.43   0.12   
    212
    Figure US20200115354A1-20200416-C00841
         		0.11   0.00659 
    213
    Figure US20200115354A1-20200416-C00842
         		0.218   0.0139  
    214
    Figure US20200115354A1-20200416-C00843
         		0.0531  0.00209 
    215
    Figure US20200115354A1-20200416-C00844
         		2.0    0.286  
    216
    Figure US20200115354A1-20200416-C00845
         		0.0721  0.00477 
    217
    Figure US20200115354A1-20200416-C00846
         		1.19   0.112  
    218
    Figure US20200115354A1-20200416-C00847
         		0.0209  0.0016  
    219
    Figure US20200115354A1-20200416-C00848
         		0.00171  0.000942 
    220
    Figure US20200115354A1-20200416-C00849
         		0.000418 
    221
    Figure US20200115354A1-20200416-C00850
         		0.298   0.0629  
    222
    Figure US20200115354A1-20200416-C00851
         		0.464   0.124  
    223
    Figure US20200115354A1-20200416-C00852
         		0.00973  0.00145 
    224
    Figure US20200115354A1-20200416-C00853
         		0.00833  0.00236 
    225
    Figure US20200115354A1-20200416-C00854
         		0.000643 0.0006514
    226
    Figure US20200115354A1-20200416-C00855
         		0.00145  0.00104 
    227
    Figure US20200115354A1-20200416-C00856
         		0.00069  0.00119 
    228
    Figure US20200115354A1-20200416-C00857
         		0.00578  0.000846 
    229
    Figure US20200115354A1-20200416-C00858
         		0.055   0.00838 
    230
    Figure US20200115354A1-20200416-C00859
         		0.000627 0.00152 
    231
    Figure US20200115354A1-20200416-C00860
         		0.00222  0.000933 
    232
    Figure US20200115354A1-20200416-C00861
         		0.0478  0.0105  
    233
    Figure US20200115354A1-20200416-C00862
         		0.00982  0.00171 
    234
    Figure US20200115354A1-20200416-C00863
         		2.0    0.131  
    235
    Figure US20200115354A1-20200416-C00864
         		0.0118  0.00814 
    236
    Figure US20200115354A1-20200416-C00865
         		0.000593 0.0016  
    237
    Figure US20200115354A1-20200416-C00866
         		0.00243  0.00107 
    238
    Figure US20200115354A1-20200416-C00867
         		0.00554  0.00117 
    239
    Figure US20200115354A1-20200416-C00868
         		0.00293  0.0015  
    240
    Figure US20200115354A1-20200416-C00869
         		0.015   0.0029  
    241
    Figure US20200115354A1-20200416-C00870
         		0.00373  0.00148 
    242
    Figure US20200115354A1-20200416-C00871
         		0.000428 0.000182 
    • Example 244 Analgesia Induced by Sodium Channel Blockers Heat Induced Tail Flick Latency Test
  • In this test, the analgesia effect produced by administering a compound of the invention can be observed through heat-induced tail-flick in mice. The test includes a heat source consisting of a projector lamp with a light beam focused and directed to a point on the tail of a mouse being tested. The tail-flick latencies, which are assessed prior to drug treatment, and in response to a noxious heat stimulus, i.e., the response time from applying radiant heat on the dorsal surface of the tail to the occurrence of tail flick, are measured and recorded at 40, 80, 120, and 160 minutes.
  • For the first part of this study, 65 animals undergo assessment of baseline tail flick latency once a day over two consecutive days. These animals are then randomly assigned to one of the 11 different treatment groups including a vehicle control, a morphine control, and 9 compounds at 30 mg/Kg are administered intramuscularly. Following dose administration, the animals are closely monitored for signs of toxicity including tremor or seizure, hyperactivity, shallow, rapid or depressed breathing and failure to groom. The optimal incubation time for each compound is determined via regression analysis. The analgesic activity of the test compounds is expressed as a percentage of the maximum possible effect (% MPE) and is calculated using the following formula:
  • % MPE Postdrug latency - Predrug latency Cut - off time ( 10 s ) - Predrug latency × 100 %
  • where:
  • Postdrug latency=the latency time for each individual animal taken before the tail is removed (flicked) from the heat source after receiving drug.
  • Predrug latency=the latency time for each individual animal taken before the tail is flicked from the heat source prior to receiving drug.
  • Cut-off time (10 s)=is the maximum exposure to the heat source.
  • Acute Pain (Formalin Test)
  • The formalin test is used as an animal model of acute pain. In the formalin test, animals are briefly habituated to the plexiglass test chamber on the day prior to experimental day for 20 minutes. On the test day, animals are randomly injected with the test articles. At 30 minutes after drug administration, 50 μL of 10% formalin is injected subcutaneously into the plantar surface of the left hind paw of the rats. Video data acquisition begins immediately after formalin administration, for duration of 90 minutes.
  • The images are captured using the Actimetrix Limelight software which stores files under the *.llii extension, and then converts it into the MPEG-4 coding. The videos are then analyzed using behaviour analysis software “The Observer 5.1”, (Version 5.0, Noldus Information Technology, Wageningen, The Netherlands). The video analysis is conducted by watching the animal behaviour and scoring each according to type, and defining the length of the behaviour (Dubuisson and Dennis, 1977). Scored behaviours include: (1) normal behaviour, (2) putting no weight on the paw, (3) raising the paw, (4) licking/biting or scratching the paw. Elevation, favoring, or excessive licking, biting and scratching of the injected paw indicate a pain response. Analgesic response or protection from compounds is indicated if both paws are resting on the floor with no obvious favoring, excessive licking, biting or scratching of the injected paw.
  • Analysis of the formalin test data is done according to two factors: (1) Percent Maximal Potential Inhibitory Effect (% MPIE) and (2) pain score. The % MPIEs is calculated by a series of steps, where the first is to sum the length of non-normal behaviours (behaviours 1, 2, 3) of each animal. A single value for the vehicle group is obtained by averaging all scores within the vehicle treatment group. The following calculation yields the MPIE value for each animal:

  • MPIE (%)=100−[(treatment sum/average vehicle value)×100%]
  • The pain score is calculated from a weighted scale as described above. The duration of the behaviour is multiplied by the weight (rating of the severity of the response), and divided by the total length of observation to determine a pain rating for each animal. The calculation is represented by the following formula:

  • Pain rating=[0(To)+1(T1)+2(T2)+3(T3)]/(To+T1+T2+T3)

  • CFA Induced Chronic Inflammatory Pain
  • In this test, tactile allodynia is assessed with calibrated von Frey filaments. Following a full week of acclimatization to the vivarium facility, 150 μL of the “Complete Freund's Adjuvant” (CFA) emulsion (CFA suspended in an oil/saline (1:1) emulsion at a concentration of 0.5 mg/mL) is injected subcutaneously into the plantar surface of the left hind paw of rats under light isoflurane anaesthesia. Animals are allowed to recover from the anaesthesia and the baseline thermal and mechanical nociceptive thresholds of all animals are assessed one week after the administration of CFA. All animals are habituated to the experimental equipment for 20 minutes on the day prior to the start of the experiment. The test and control articles are administrated to the animals, and the nociceptive thresholds measured at defined time points after drug administration to determine the analgesic responses to each of the six available treatments. The time points used are previously determined to show the highest analgesic effect for each test compound.
  • Thermal nociceptive thresholds of the animals are assessed using the Hargreaves test. Animals are placed in a Plexiglas enclosure set on top of an elevated glass platform with heating units. The glass platform is thermostatically controlled at a temperature of approximately 30° C. for all test trials. Animals are allowed to accommodate for 20 minutes following placement into the enclosure until all exploration behaviour ceases. The Model 226 Plantar/Tail Stimulator Analgesia Meter (IITC, Woodland Hills, Calif.) is used to apply a radiant heat beam from underneath the glass platform to the plantar surface of the hind paws. During all test trials, the idle intensity and active intensity of the heat source are set at 1 and 45 respectively, and a cut off time of 20 seconds is employed to prevent tissue damage.
  • The response thresholds of animals to tactile stimuli are measured using the Model 2290 Electrovonfrey anesthesiometer (IITC Life Science, Woodland Hills, Calif.) following the Hargreaves test. Animals are placed in an elevated Plexiglas enclosure set on a mire mesh surface. After 10 minutes of accommodation, pre-calibrated Von Frey hairs are applied perpendicularly to the plantar surface of both paws of the animals in an ascending order starting from the 0.1 g hair, with sufficient force to cause slight buckling of the hair against the paw. Testing continues until the hair with the lowest force to induce a rapid flicking of the paw is determined or when the cut off force of approximately 20 g is reached. This cut off force is used because it represent approximately 10% of the animals' body weight and it serves to prevent raising of the entire limb due to the use of stiffer hairs, which would change the nature of the stimulus.
    • Postoperative Models of Nociception
  • In this model, the hypealgesia caused by an intra-planar incision in the paw is measured by applying increased tactile stimuli to the paw until the animal withdraws its paw from the applied stimuli. While animals are anaesthetized under 3.5% isofluorane, which is delivered via a nose cone, a 1 cm longitudinal incision is made using a number 10 scalpel blade in the plantar aspect of the left hind paw through the skin and fascia, starting 0.5 cm from the proximal edge of the heel and extending towards the toes. Following the incision, the skin is apposed using 2, 3-0 sterilized silk sutures. The injured site is covered with Polysporin and Betadine. Animals are returned to their home cage for overnight recovery.
  • The withdrawal thresholds of animals to tactile stimuli for both operated (ipsilateral) and unoperated (contralateral) paws can be measured using the Model 2290 Electrovonfrey anesthesiometer (IITC Life Science, Woodland Hills, Calif.). Animals are placed in an elevated Plexiglas enclosure set on a mire mesh surface. After at least 10 minutes of acclimatization, pre-calibrated Von Frey hairs are applied perpendicularly to the plantar surface of both paws of the animals in an ascending order starting from the 10 g hair, with sufficient force to cause slight buckling of the hair against the paw. Testing continues until the hair with the lowest force to induce a rapid flicking of the paw is determined or when the cut off force of approximately 20 g is reached. This cut off force is used because it represent approximately 10% of the animals' body weight and it serves to prevent raising of the entire limb due to the use of stiffer hairs, which would change the nature of the stimulus.
    • Neuropathic Pain Model; Chronic Constriction Injury
  • Briefly, an approximately 3 cm incision is made through the skin and the fascia at the mid thigh level of the animals' left hind leg using a no. 10 scalpel blade. The left sciatic nerve is exposed via blunt dissection through the biceps femoris with care to minimize haemorrhagia. Four loose ligatures are tied along the sciatic nerve using 4-0 non-degradable sterilized silk sutures at intervals of 1 to 2 mm apart. The tension of the loose ligatures is tight enough to induce slight constriction of the sciatic nerve when viewed under a dissection microscope at a magnification of 4 fold. In the sham-operated animal, the left sciatic nerve is exposed without further manipulation. Antibacterial ointment is applied directly into the wound, and the muscle is closed using sterilized sutures. Betadine is applied onto the muscle and its surroundings, followed by skin closure with surgical clips.
  • The response thresholds of animals to tactile stimuli are measured using the Model 2290 Electrovonfrey anesthesiometer (IITC Life Science, Woodland Hills, Calif.). Animals are placed in an elevated Plexiglas enclosure set on a mire mesh surface. After 10 minutes of accommodation, pre-calibrated Von Frey hairs are applied perpendicularly to the plantar surface of both paws of the animals in an ascending order starting from the 0.1 g hair, with sufficient force to cause slight buckling of the hair against the paw. Testing continues until the hair with the lowest force to induce a rapid flicking of the paw is determined or when the cut off force of approximately 20 g is reached. This cut off force is used because it represents approximately 10% of the animals' body weight and it serves to prevent raising of the entire limb due to the use of stiffer hairs, which would change the nature of the stimulus.
  • Thermal nociceptive thresholds of the animals are assessed using the Hargreaves test. Following the measurement of tactile thresholds, animals are placed in a Plexiglass enclosure set on top of an elevated glass platform with heating units. The glass platform is thermostatically controlled at a temperature of approximately 24 to 26° C. for all test trials. Animals are allowed to accommodate for 10 minutes following placement into the enclosure until all exploration behaviour ceases. The Model 226 Plantar/Tail Stimulator Analgesia Meter (IITC, Woodland Hills, Calif.) is used to apply a radiant heat beam from underneath the glass platform to the plantar surface of the hind paws. During all test trials, the idle intensity and active intensity of the heat source are set at 1 and 55 respectively, and a cut off time of 20 seconds is used to prevent tissue damage.
    • Neuropathic Pain Model: Spinal Nerve Ligation
  • The spinal nerve ligation (SNL) neuropathic pain model is used as an animal (i.e. rat) model of neuropathic pain. In the SNL test, the lumbar roots of spinal nerves L5 and L6 are tightly ligated to cause nerve injury, which results in the development of mechanical hyperalgesia, mechanical allodynia and thermal hypersensitivity. The surgery is performed two weeks before the test day in order for the pain state to fully develop in the animals. Several spinal nerve ligation variations are used to characterize the analgesic properties of a compound of the invention.
  • Ligation of the L5 spinal nerve;
  • Ligation of the L5 and L6 spinal nerves;
  • Ligation and transection of the L5 spinal nerve;
  • Ligation and transection of the L5 and L6 spinal nerves; or
  • Mild irritation of the L4 spinal nerve in combination with any one of the above (1)-(4).
  • While the animals are anaesthetized under 3.5% isofluorane delivered via a nose cone, an approximately 2.5 cm longitudinal incision is made using a number 10 scalpel blade in the skin just lateral to the dorsal midline, using the level of the posterior iliac crests as the midpoint of the incision. Following the incision, the isoflourane is readjusted to maintenance levels (1.5%-2.5%). At mid-sacral region, an incision is made with the scalpel blade, sliding the blade along the side of the vertebral column (in the saggital plane) until the blade hits the sacrum. Scissors tips are introduced through the incision and the muscle and ligaments are removed from the spine to expose 2-3 cm of the vertebral column. The muscle and fascia are cleared from the spinal vertebra in order to locate the point where the nerve exits from the vertebra. A small glass hook is placed medial to the spinal nerves and the spinal nerves are gently elevated from the surrounding tissues. Once the spinal nerves have been isolated, a small length of non-degradable 6-0 sterilized silk thread is wound twice around the ball at the tip of the glass hook and passed back under the nerve. The spinal nerves are then firmly ligated by tying a knot, ensuring that the nerve bulges on both sides of the ligature. The procedure may be repeated as needed. In some animals, the L4 spinal nerve may be lightly rubbed (up to 20 times) with the small glass hook to maximize the development of neuropathic pain. Antibacterial ointment is applied directly into the incision, and the muscle is closed using sterilized sutures. Betadine is applied onto the muscle and its surroundings, followed by skin closure with surgical staples or sterile non-absorable monofilament 5-0 nylon sutures.
  • The analgesic effect produced by topical administration of a compound of the invention to the animals can then be observed by measuring the paw withdrawal threshold of animals to mechanical tactile stimuli. These may be measured using either the mechanical allodynia procedure or the mechanical hyperalgesia procedure as described below. After establishment of the appropriate baseline measurements by either method, topical formulation of a compound of the invention is applied on the ipsilateral ankle and foot. The animals are then placed in plastic tunnels for 15 minutes to prevent them from licking the treated area and removing the compound. Animals are placed in the acrylic enclosure for 15 minutes before testing the ipsilateral paw by either of the methods described below, and the responses are recorded at 0.5, 1.0 and 2.0 hour post treatment.
    • Mechanical Allodynia Method
  • The pain threshold of animals to mechanical alloydnia for both operated and control animals can be measured approximately 14 days post-surgery using manual calibrated von Frey filaments as follows. Animals are placed in an elevated plexiglass enclosure set on a mire mesh surface. Animals are allowed to acclimate for 20-30 minutes. Pre-calibrated Von Frey hairs are applied perpendicularly to the plantar surface of the ipsilateral paw of the animals starting from the 2.0 g hair, with sufficient force to cause slight buckling of the hair against the paw to establish the baseline measurements. Stimuli are presented in a consecutive manner, either in an ascending or descending order until the first change in response is noted, after which four additional responses are recorded for a total of six responses. The six responses measured in grams are entered into a formula as described by Chaplan, S. R. et al., J. Neurosci. Methods, 1994 July; 53(1):55-63, and a 50% withdrawal threshold is calculated. This constitutes the mechanical allodynia value.
    • Mechanical Hyperalgesia Method
  • The response thresholds of animals to tactile stimuli are measured using the Model 2290 Electrovonfrey anesthesiometer (IITC Life Science, Woodland Hills, Calif.). Animals are placed in an elevated Plexiglas enclosure set on a wire mesh surface. After 15 minutes of accommodation in this enclosure, a von Frey hair is applied perpendicularly to the plantar surface of the ipsilateral hind paws of the animals, with sufficient force, measured in grams, to elicit a crisp response of the paw. A response indicates a withdrawal from the painful stimulus and constitutes the efficacy endpoint. The data are expressed as percent change from baseline threshold measured in grams.
    • Example 245 In Vivo Assay for Treatment of Pruritus
  • The compounds of the invention can be evaluated for their activity as antipruritic agents by in vivo test using rodent models. One established model for peripherally elicited pruritus is through the injection of serotonin into the rostral back area (neck) in hairless rats. Prior to serotonin injections (e.g., 2 mg/mL, 50 μL), a dose of a compound of the present invention can be applied systemically through oral, intravenous or intraperitoneal routes or topically to a circular area fixed diameter (e.g. 18 mm). Following dosing, the serotonin injections are given in the area of the topical dosing. After serotonin injection the animal behaviour is monitored by video recording for 20 min-1.5 h, and the number of scratches in this time compared to vehicle treated animals. Thus, application of a compound of the current invention could suppress serotonin-induced scratching in rats.
  • All of the U.S. patents, U.S. patent application publications, U.S. patent applications, foreign patents, foreign patent applications and non patent publications referred to in this specification are incorporated herein by reference in their entireties.
  • Although the foregoing invention has been described in some detail to facilitate understanding, it will be apparent that certain changes and modifications may be practiced within the scope of the appended claims. Accordingly, the described embodiments are to be considered as illustrative and not restrictive, and the invention is not to be limited to the details given herein, but may be modified within the scope and equivalents of the appended claims.

Claims (17)

1-2. (canceled)
3. A compound of formula Ia:
Figure US20200115354A1-20200416-C00872
wherein:
R1, R2, and R3 are each independently selected from hydrogen, C1-C6 alkyl, cyano, halo, and C1-C6 haloalkyl; and R4 and Z together with the benzene ring to which they are both attached form a 9- or 10-membered heterobicycle;
Het is 5- or 6-membered heteroaryl optionally substituted with one to three substituents each independently selected from C1-C6 alkyl, cyano, halo, and C1-C6 haloalkyl;
X is selected from —N(R6)2 and —N(R7)3 + −W, and amine oxides thereof;
Y1 is —C(R8)2—; and Y2 is selected from —(C(R8)2)n—, —N(R9)—, —O—, —C(R8)2—N(R9)—, —N(R9)—C(R8)2—, —C(R8)2—O—, and —O—C(R8)2—; or
Y1 is selected from —N(R9)— and —O—, and Y2 is —(C(R8)2)n—;
n is selected from 0, 1, and 2;
Y3 and Y6 are each —C(R8)2—;
Y4 and Y5 are each —C(R8)—;
each R6 is independently selected from hydrogen, C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkanoyl, 4-7 membered heterocycle, 5-6 membered heteroaryl, and C6-C12 aryl, wherein any C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkanoyl, 4-7 membered heterocycle, 5-6 membered heteroaryl, and C6-C12 aryl is optionally substituted with one or more groups independently selected from deuterium, halo, cyano, hydroxy, C1-C6 alkyl, C1-C6 alkoxy, C1-C6 alkanoyl, C1-C6 haloalkyl, C1-C6 haloalkoxy, C3-C6 cycloalkyl, C3-C6 halocycloalkyl, —NRaRb, —C(═O)NRaRb, Rc, and phenyl, wherein any C1-C6 alkyl, C1-C6 alkoxy, and C1-C6 alkanoyl is optionally substituted with C3-C6 cycloalkyl; or two R6 groups together with the nitrogen to which they are both attached form a 4- to 10-membered heterocycle that is optionally substituted with one or more groups independently selected from deuterium, halo, cyano, hydroxy, C1-C6 alkyl, and C3-C6 cycloalkyl, which C1-C6 alkyl, and C3-C6 cycloalkyl is optionally substituted with one or more groups independently selected from hydroxy and halo;
each R7 is independently selected from C1-C6 alkyl; or two R7 groups together with the nitrogen to which they are both attached form a 4- to 7-membered heterocycle;
each R8 is independently selected from hydrogen, C1-C6 alkyl, C1-C6 haloalkyl, C3-C6 cycloalkyl, benzyl, 5-15 membered heteroaryl, C1-C6 alkoxy, C1-C6 haloalkoxy, C3-C6 cycloalkoxy, hydroxy, halo, cyano, and -L-C6-C12 aryl; wherein each C3-C6 cycloalkyl, -L-C6-C12 aryl, benzyl, 5-15 membered heteroaryl, and C1-C6 alkoxy, is optionally substituted with one to three substituents Rx each independently selected from C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkoxy, cyano, halo, hydroxy, C3-C6 cycloalkoxy, C3-C6 halocycloalkyl, —S(C1-C6 alkyl), —S(O)(C1-C6 alkyl), —S(O)2(C1-C6 alkyl), —NH2—NH(C1-C6 alkyl), —N(C1-C6 alkyl)2, 4-6 membered heterocycle, and C1-C6 haloalkyl or two R8 that are on adjacent carbons taken together form a double bond; and R9 is selected from hydrogen, C1-C6 alkyl and C6-C12 aryl, which C6-C12 aryl is optionally substituted with one to three substituents each independently selected from C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkoxy, cyano, halo, and C1-C6 haloalkyl; or one R8 or R9 taken together with another R8 or R9 and the atoms to which they are attached form a 3-8 membered fused, bridged or spirocyclic ring, which 3-8 membered ring is optionally substituted with one to three substituents each independently selected from C1-C6 alkyl, C1-C6 alkoxy, cyano, halo, and C1-C6 haloalkyl; and L is selected from a bond, —O—, —S—, —S(O)—, and —S(O)2—;
each Ra and Rb is independently selected from the group consisting of hydrogen, C1-C6 alkyl, and C1-C6 alkanoyl;
each Rc is independently selected from 4-7 membered heterocycle that is optionally substituted with one or more groups independently selected from halo, C1-C6 alkyl, and C1-C6 haloalkyl; and
W− is a counterion,
or a pharmaceutically acceptable salt thereof.
4. The compound of claim 3, which is a compound of formula Ib:
Figure US20200115354A1-20200416-C00873
or a pharmaceutically acceptable salt thereof.
5. The compound of claim 3, which is a compound of formula Ic:
Figure US20200115354A1-20200416-C00874
or a pharmaceutically acceptable salt thereof.
6. The compound of claim 3, wherein one of Y1 and Y2 is —CH(R8)— and the other of Y1 and Y2 is —CH2—; R8 is not H; Y3 and Y6 are each —CH2—; and Y4 and Y5 are each —CH—; or a pharmaceutically acceptable salt thereof.
7-11. (canceled)
12. The compound of claim 6, wherein R8 is trifluoromethyl or phenyl that is optionally substituted with one to three substituents each independently selected from C1-C6 alkyl, cyano, halo, and C1-C6 haloalkyl; or a pharmaceutically acceptable salt thereof.
13. The compound of claim 6, wherein R8 is trifluoromethyl or 3-trifluoromethylphenyl; or a pharmaceutically acceptable salt thereof.
14. The compound of claim 6, wherein R8 is phenyl that is optionally substituted with one to three substituents Rx each independently selected from the group consisting of C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkoxy, cyano, halo, hydroxy, C3-C6 cycloalkoxy, C3-C6 halocycloalkyl, —S(C1-C6 alkyl), —S(O)(C1-C6 alkyl), —S(O)2(C1-C6 alkyl), —NH2—NH(C1-C6 alkyl), —N(C1-C6 alkyl)2, 4-6 membered heterocycle, and C1-C6 haloalkyl; or a pharmaceutically acceptable salt thereof.
15. The compound of claim 6, wherein R8 is phenyl that is optionally substituted with one to three substituents Rx each independently selected from the group consisting of C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkoxy, cyano, halo, and C1-C6 haloalkyl; or a pharmaceutically acceptable salt thereof.
16. The compound of claim 6, wherein R8 is phenyl that is optionally substituted with one to three substituents Rx each independently selected from the group consisting of F, Cl, CN, C1-C6 alkyl, C3-C6 cycloalkyl, C1-C6 alkoxy, C1-C6 haloalkyl, and C1-C6 haloalkoxy; or a pharmaceutically acceptable salt thereof.
17. The compound of claim 6, wherein R8 is phenyl that is optionally substituted with one to three substituents Rx each independently selected from the group consisting of fluoro, chloro, trifluoromethyl, propyl, cyclopropyl, and trifluoromethoxy; or a pharmaceutically acceptable salt thereof.
18-47. (canceled)
48. A pharmaceutical composition comprising a compound of claim 3, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.
49-56. (canceled)
57. The compound of claim 4, wherein one of Y1 and Y2 is —CH(R8)— and the other of Y1 and Y2 is —CH2—; R8 is not H; Y3 and Y6 are each —CH2—; and Y4 and Y5 are each —CH—; or a pharmaceutically acceptable salt thereof.
58. The compound of claim 5, wherein one of Y1 and Y2 is —CH(R8)— and the other of Y1 and Y2 is —CH2—; R8 is not H; Y3 and Y6 are each —CH2—; and Y4 and Y5 are each —CH—; or a pharmaceutically acceptable salt thereof.
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