US20160243102A1

US20160243102A1 - Rho kinase inhibitors

Info

Publication number: US20160243102A1
Application number: US15/142,448
Authority: US
Inventors: Todd Bosanac; John David Ginn; Eugene Richard Hickey; Thomas Martin Kirrane, Jr.; Weimin Liu; Anthony S. Prokopowicz; Sabine K. Schlyer; Cheng-Kon Shih; Roger John Snow; Michael Robert Turner; Frank Wu; Erick Richard Young
Original assignee: Aerie Pharmaceuticals Inc
Current assignee: Alcon Inc
Priority date: 2006-09-20
Filing date: 2016-04-29
Publication date: 2016-08-25
Also published as: CA2664335A1; US10624882B2; CA2664335C; AR062900A1; EP2068878A2; ES2729424T3; US20180344723A1; US8809326B2; US20140357652A1; EP2526948A1; WO2008036540A3; CL2007002686A1; EP2068878B1; US20150297581A1; WO2008036540A2; US20080161297A1; JP2010504342A; TW200819133A; JP5235887B2

Abstract

Disclosed are novel substituted 2H-isoquinolin-1-one and 3H-quinazolin-4-one derivatives useful as inhibitors of Rho kinase and for treating a variety of diseases and disorders that are mediated or sustained through the activity of Rho kinase, including cardiovascular diseases, pharmaceutical compositions comprising such compounds, methods for using such compounds and processes for making such compounds.

Description

FIELD OF THE INVENTION

This invention relates to substituted 2H-isoquinolin-1-one and 3H-quinazolin-4-one derivatives which are useful as inhibitors of Rho kinase and are thus useful for treating a variety of diseases and disorders that are mediated or sustained through the activity of Rho kinase, including cardiovascular diseases, cancer, neurological diseases, renal diseases, bronchial asthma, erectile dysfunction, and glaucoma. This invention also relates to pharmaceutical compositions comprising these compounds, methods of using these compounds in the treatment of various diseases and disorders, processes for preparing these compounds and intermediates useful in these processes.

BACKGROUND

Rho-Kinase (ROCK) is a member of the serine-threonine protein kinase family. ROCK exists in two isoforms, ROCK1 and ROCK2 (T. Ishizaki et al., EMBO J., 1996, 15, 1885-1893). ROCK has been identified as an effector molecule of RhoA, a small GTP-binding protein (G protein) that plays a key role in multiple cellular signaling pathways. ROCK and RhoA are ubiquitously expressed across tissues. The RhoA/ROCK signaling pathway is involved in a number of cellular functions, such as acting organization, cell adhesion, cell migration, and cytokinesis (K. Riento and A. J. Ridley, Nat Rev Mol Cell Biol, 2003, 4, 446-56). It is also directly involved in regulating smooth muscle contraction (A. P. Somlyo, Nature, 1997, 389, 908-911). Upon activation of its receptor, RhoA is activated and in turn it activates ROCK. Activated ROCK phosphorylates the myosin-binding subunit of myosin light chain phosphatase, which inhibits activity of the phosphatase and leads to contraction. Contraction of the smooth muscle in the vasculature increases blood pressure, leading to hypertension.
There is considerable evidence in the literature that the Rho/ROCK signaling pathway plays an important role in signal transduction initiated by several vasoactive factors, for example angiotensin II, urotension II, endothelin-1, serotonin, norepinephrine and platelet-derived growth factor (PDGF). Many of these factors are implicated in the pathogenesis of cardiovascular disease.
Additional studies in the literature, some using known ROCK inhibitors fasudil (T. Asano et al., J. Pharmacol. Exp. Ther., 1987, 24, 1033-1040) or Y-27632 (M. Uehata et al., Nature, 1997, 389, 990-994) further illustrate the link between ROCK and cardiovascular disease. For example, ROCK expression and activity have been shown to be elevated in spontaneously hypertensive rats, suggesting a link to the development of hypertension in these animals. The ROCK inhibitor Y-27632 (M. Uehata et al., Nature, ibid) was shown to significantly decrease blood pressure in three rat models of hypertension, including the spontaneously hypertensive rat, renal hypertensive rat and deoxycortisone acetate salt hypertensive rat models while having only a minor effect on blood pressure in control rats, reinforcing the link between ROCK and hypertension.
Other studies suggest a link between ROCK and atherosclerosis. For example, gene transfer of a dominant negative form of ROCK suppressed neointimal formation following balloon injury in porcine femoral arteries. In a similar model, ROCK inhibitor Y-27632 also inhibited neointimal formation in rats. In a porcine model of IL-1 beta-induced coronary stenosis, long term treatment by ROCK inhibitor fasudil was shown to progressively reduce coronary stenosis as well as promote a regression of coronary constrictive remodeling.
Additional investigations suggest that a ROCK inhibitor would be useful in treating other cardiovascular diseases. For example, in a rat stroke model, fasudil was shown to reduce both the infarct size and neurologic deficit. The ROCK inhibitor Y-27632 was shown to improve ventricular hypertrophy and function in a model of congestive heart failure in Dahl salt-sensitive rats.
Other animal or clinical studies have implicated ROCK in additional discuses including coronary vasospasm, cerebral vasospasm, ischemia/reperfusion injury, pulmonary hypertension, angina, renal disease and erectile dysfunction.
The above studies provide evidence for a link between ROCK and cardiovascular diseases including hypertension, atherosclerosis, restenosis, stroke, heart failure, coronary vasospasm, cerebral vasospasm, ischemia/reperfusion injury, pulmonary hypertension and angina, as well as renal disease and erectile dysfunction. Given the demonstrated effect of ROCK on smooth muscle, ROCK inhibitors may also be useful in other diseases involving smooth muscle hyper reactivity, including asthma and glaucoma. Furthermore, Rho-kinase has been indicated as a drug target for the treatment of various other diseases, including airway inflammation and hyperresponsiveness, cancer, as well as neurological disorders, such as spinal-cord injury, Alzheimer disease, multiple sclerosis, stroke and neuropathic pain.
There remains an unmet medical need for new drugs to treat cardiovascular disease. A study published in 2003 estimated that almost 29% of the adult U.S. population had hypertension in 1999-2000 (I. Hajjar et al., JAMA, 2003, 290, 199-206). Furthermore, 69% of the hypertensive individuals studied during this period did not have their hypertension controlled at the time their blood pressure was measured. This figure was worse in hypertensive patients with diabetes, where 75% of those patients studied did not have their blood pressure controlled to the target level. Another more recent study showed similar results, with less than one-third of hypertensive patients studied having blood pressure controlled to the target level (V. Andros, Am. J. Manag. Care, 2005, 11, S215-S219). Therefore, despite the number of medications available to treat hypertension, including diuretics, beta blockers, angiotensin converting enzyme inhibitors, angiotensin blockers and calcium channel blockers, hypertension remains poorly controlled or resistant to current medication for many patients. If not adequately treated, hypertension can lead to other cardiovascular diseases and organ failure including coronary artery disease, stroke, myocardial infarction, cardiac failure, renal failure and peripheral artery disease.
Although there are many reports of ROCK inhibitors under investigation (see, for example, E. Hu and D. Lee, Expert Opin. Ther. Targets, 2005, 9, 715-736), so far fasudil is the only marketed ROCK inhibitor. An i.v. formulation of fasudil was approved in Japan for treatment of cerebral vasospasm. There remains a need for new therapeutics, including ROCK inhibitors, for the treatment of cardiovascular diseases, cancer, neurological diseases, renal diseases, bronchial asthma, erectile dysfunction, and glaucoma.

BRIEF SUMMARY OF THE INVENTION

In a general aspect, the present invention is directed to the compounds of the following formula (I):
wherein R₁, R₂, X and Y are as defined herein, as well as the tautomers thereof, and salts thereof. It has been found that the compounds of formula (I) have valuable pharmacological properties, particularly an inhibiting activity on Rho kinase.
In another aspect, the present invention is directed to a method of inhibiting Rho kinase activity in an individual comprising administering to the individual a compound described above.
In another aspect, the present invention is directed to a method of treating a disease or disorder associated with the activation of Rho kinase comprising administering to an individual a compound described above.
In another aspect, the present invention is directed to a method of treating a cardiovascular or disease or condition comprising administering to an individual a compound described above. Examples of such diseases that may be treated include, for example, hypertension, atherosclerosis, stroke, heart failure, restenosis, myocaridial infarction, organ failure, renal failure, coronary artery disease, peripheral artery disease, coronary vasospasm, cerebral vasospasm, ischemia/reperfusion injury, pulmonary hypertension, angina, erectile dysfunction and renal disease.
In another aspect, the present invention is directed to a method of treating diseases involving smooth muscle hyper reactivity including asthma and glaucoma, comprising administering to an individual a compound described above.
In another aspect, the present invention is directed to a method of treating diseases involving Rho-kinase under pathophysiological conditions, including airway inflammation and hyper-responsiveness, cancer, and various neurological diseases, comprising administering to an individual in need of such treatment a compound of the present invention as described above.
In yet additional aspects, the present invention is directed to pharmaceutical compositions comprising the above-mentioned compounds, processes for preparing the above-mentioned compounds and intermediates used in these processes.

DETAILED DESCRIPTION OF THE INVENTION

In an embodiment, there is provided compounds of the formula (I)

- wherein:
- R₁is chosen from
- C_1-6alkyl, C_3-8cycloalkyl, C_3-8cycloalkylC_1-6alkyl, haloC_1-6alkyl, aminoC_1-6alkyl, hydroxyC_1-6alkyl, aryl, arylC_1-6alkyl, heteroaryl, heteroarylC_1-6alkyl, heterocyclyl, —C_1-3alkylOaryl, —C(H)_0-1(C_1-6alkyl)_1-2aryl, —CH(OH)aryl, —C(OH)(CH₃)aryl, —CH[OC(O)C_1-6alkyl]aryl, —CH₂OCH₂aryl, —CH₂OC(O)C_1-6alkyl, —(CH₂)_1-3S(O)_0-2aryl, —(CH₂)_1-2S(O)_0-2C_1-6alkyl, —(CH₂)_1-3CO₂C_1-6alkyl, —(CH₂)_1-3NHC_1-6alkyl, —(CH₂)_1-3NHC_1-6alkylC_3-8cycloalkyl, —(CH₂)_1-2CN and —CH(R₃)N(R₄)(R₅)
- wherein:
- R₃is chosen from
- H, aryl, C_1-6alkyl, —(CH₂)_1-3aryl and —(CH₂)_1-3heteroaryl;
- R₄is chosen from
- H, C_1-6alkyl, C_3-12cycloalkyl, C_3-7cycloalkylC_1-6alkyl, aryl, aryl(CH₂)_1-3, heteroaryl(CH₂)_1-3, C_1-3alkylO(CH₂)_1-3, tetrahydropyran-4-ylmethyl and C_1-3alkyl)₂N(CH₂)_2-4—;
- and R₅is chosen from
- H and C_1-6alkyl;
- or R₄and R₅together with the nitrogen atom they are connected to may form a heterocyclyl group;
- wherein each aryl, arylalkyl, heteroaryl, heteroarylalkyl, cycloalkylalkyl, cycloalkyl and heterocyclyl group is optionally substituted with 1-3 groups selected from
- halogen, C_1-6alkoxy, C_3-8cycloalkyl, C_1-6alkyl, —CN, —NO₂, —OH, oxo, —CF₃, —OCF₃, —C_0-3, alkylCO₂H, C_1-6alkylCO₂—, C_1-6alkylsulfonylC_0-3alkyl-, —SO₂C_-1-6alkylNR₆R₇, —C_0-3alkylSO₂NR₆R₇, —C_0-3C(O)NR₆R₇, aryl, heteroaryl, heteroarylC_1-3alkyl, heterocyclyl, heterocyclylSO₂—, arylC_1-3alkyl, aryloxy, arylthio and C_0-3NR₆R₇;
- wherein each aryl and heteroaryl group is optionally substituted with 1-3 groups selected from
- halogen, C_1-6alkoxy, C_1-6alkyl, —CN, —NO₂, —OH, —CF₃, —OCF₃, —C(O)NR₈R₉, —SO₂NR₈R₉, —SO₂Me and amino optionally substituted by one or two C_1-6alkyl groups or a C(O)C_1-6alkyl group;
- R₂is chosen from H, halogen, C_1-6alkoxy, —CN, —CF₃and C_1-6alkyl;
- R₆and R₇are independently selected from H, C_1-6alkyl, —C(O)C_1-6alkyl and —C_1-6alkylNH₂; or
- R₆and R₇, together with the nitrogen they ace connected to may form a piperazine, piperidine or pyrrolidine ring;
- R₈and R₉are independently selected from H and methyl;
- R₁₀is selected from H, Cl and F;
- X is chosen from C and N; and
- Y is chosen from —NHC(O)—, —NHC(O)NH— and —NHC(O)O—;
- or a tautomer thereof or a salt thereof, preferably a pharmaceutically acceptable salt thereof.

Preferably if R₁is a C_1-6alkyl, and Y is —NHC(O)—, then R₁is not a methyl group.
In an embodiment, there is provided compounds of the formula (I)

- wherein:
- R₁is chosen from
- C_3-8cycloalkyl, C_3-8cycloalkylC_1-6alkyl, haloC_1-6alkyl, aminoC_1-6alkyl, hydroxyC_1-6alkyl, aryl, arylC_1-6alkyl, heteroaryl, heteroarylC_1-6alkyl, heterocyclyl, —C_1-3alkylOaryl, —C(H)_0-1*(C_1-6alkyl)_1-2aryl, —CH(OH)aryl, —C(OH)(CH₃)aryl, —CH[OC(O)C_1-6alkyl]aryl, —CH₂OCH₂aryl, —CH₂OC(O)C_1-6alkyl, —(CH₂)_1-3S(O)_0-2aryl, —(CH₂)_1-2S(O)_0-2C_1-6alkyl, —(CH₂)_1-3CO₂C_1-6alkyl, —(CH₂)_1-3NHC_1-6alkyl, —(CH₂)_1-3NHC_1-6alkylC_3-8cycloalkyl, —(CH₂)_1-2CN and —CH(R₃)N(R₄)(R₅)
- wherein:
- R₃is chosen from
- H, aryl, C_1-6alkyl, —(CH₂)_1-3aryl and —(CH₂)_1-3heteroaryl;
- R₄is chosen from
- H, C_1-6alkyl, C_3-12cycloalkyl, C_3-7cycloalkylC_1-6alkyl, aryl, aryl(CH₂)_1-3, heteroaryl(CH₂)_1-3, C_1-3alkylO(CH₂)_1-3, tetrahydropyran-4-ylmethyl and (C_1-3alkyl)₂N(CH₂)_2-4-;
- and R₅is chosen from
- H and C_1-6alkyl;
- or R₄and R₅together with the nitrogen atom they are connected to may form a heterocyclyl group;
- wherein each aryl, arylalkyl, heteroaryl, heteroarylalkyl, cycloalkylalkyl, cycloalkyl and heterocyclyl group is optionally substituted with 1-3 groups selected from
- halogen, C_1-6alkoxy, C_3-8cycloalkyl, C_1-6alkyl, —CN, —NO₂, —OH, oxo, —CF₃, —OCF₃, —C_0-3alkylCO₂H, C_1-6alkylCO₂—, C_1-6alkylsulfonylC_0-3alkyl-, —SO₂C_-1-6alkylNR₆R₇, —C_0-3alkylSO₂NR₆R₇, —C_0-3C(O)NR₆R₇, aryl, heteroaryl, heteroarylC_1-3alkyl, heterocyclyl, heterocyclylSO₂—, arylC_1-3alkyl, aryloxy, arylthio and C_0-3NR₆R₇;
- wherein each aryl and heteroaryl group is optionally substituted with 1-3 groups selected from halogen, C_1-6alkoxy, C_1-6alkyl, —CN, —NO₂, —OH, —CF₃, —OCF₃, —C(O)NR₈R₉, —SO₂NR₈R₉—SO₂Me and amino optionally substituted by one or two C_1-6alkyl groups or a C(O)C_1-6alkyl group;
- R₂is chosen from H, halogen, C_1-6alkoxy, —CN, —CF₃and C_1-6alkyl;
- R₆and R₇are independently selected from H, C_1-6alkyl, —C(O)C_1-6alkyl and —C_1-6alkylNH₂; or
- R₆and R₇, together with the nitrogen they are connected to may form a piperazine, piperidine or pyrrolidine ring;
- R₈and R₉are independently selected from H and methyl;
- R₁₀is selected from H, Cl and F;
- X is chosen from C and N; and
- Y is chosen from —NHC(O)—, —NHC(O)NH— and —NHC(O)O—;
- or a tautomer thereof or a salt thereof, preferably a pharmaceutically acceptable salt.

In another embodiment, there are provided compounds of the formula (I) as described above and wherein:

- R₁is chosen from
- C_3-8cycloalkyl, C_3-8cycloalkylC_1-6alkyl, phenyl, benzyl, phenylethyl, thienyl, pyridyl, isoxazolyl, pyrazolyl, thienylmethyl, piperidinyl, piperazinyl, pyrrolidinyl, tetrahydrofuranyl, tetrahydropyranyl, —CH₂Ophenyl, —CH(C_1-3alkyl)phenyl, —CH(OH)phenyl, —C(OH)(CH₃)phenyl, —CH[OC(O)CH₃]phenyl, —CH₂OCH₂phenyl, —CH₂OC(O)C_1-6alkyl, —(CH₂)_1-3S(O)_0-2phenyl, —(CH₂)_1-2S(O)_0-2C_1-6alkyl, —(CH₂)_1-3CO₂C_1-6alkyl, —(CH₂)_1-3NHC_1-6alkyl, —(CH₂)_1-3NHC_1-6alkylC_3-8cycloalkyl, —(CH₂)_1-3CN and —CH(R₃)N(R₄)(R₅)
- wherein:
- R₃is chosen from
- H, phenyl, C_1-6alkyl, benzyl phenylethyl and pyridylmethyl;
- R₄is chosen from
- H, C_1-6alkyl, C_3-12cycloalkyl, C_3-7cycloalkylC_1-6alkyl, phenyl, benzyl, thienylethyl, C_1-3alkylO(CH₂)_1-3, tetrahydropyran-4-ylmethyl and (C_1-3)₂N(CH₂)_2-4—;
- and R₅chosen from
- H and C_1-6alkyl;
- or R₄and R₅together with the nitrogen atom they are connected to may form a piperidine, piperazine or thiomorpholine group;
- wherein each cycloalkyl, cycloalkylalkyl, phenyl, benzyl, phenylethey, thienyl, pyridyl, isoxazolyl, pyrazolyl, thienylmethyl, piperidine, piperazinyl, pyrrolidinyl, tetrahydrofuranyl, tetrahydropyranyl, and thiomorpholinyl group is optionally substituted with 1-3 groups selected from
- halogen, C_1-6alkoxy, C_1-6alkyl, —CN, —NO₂, —OH, —CF₃, —OCF₃, C_1-6alkylCO₂—, C_1-6alkylsulfonyl, phenyl, pyrimidyl, pyridyl, morpholinyl, benzyl, phenyloxy and phenylthio and amino optionally substituted by one or two C_1-6alkyl groups or a C(O)C_1-6alkyl group;
- wherein each phenyl, benzyl, pyrimidinyl and pyridyl group is optionally substituted with 1-3 groups selected from
- halogen, C_1-6alkoxy, C_1-6alkyl, —CN, —NO₂, —OH, —CF₃, —OCF₃, —C(O)NR₈R₉, —SO₂NR₈R₉, —SO₂Me and amino optionally substituted by one or two C_1-6alkyl groups or a C(O)C_1-6alkyl group;
- R₂is chosen from H, Br, Cl, —CN, —CF₃and methyl;
- R₈and R₉are independently selected from H and methyl;
- R₁₀is selected from H, Cl and F;
- X is chosen from C and N; and
- Y is chosen from —NHC(O)—, —NHC(O)NH— and —NHC(O)O—;
- or a tautomer thereof or a salt thereof, preferably a pharmaceutically acceptable salt thereof.

In a further embodiment, there are provided compounds of the formula (I) as described above and wherein:

- R₁is chosen from
- cyclopentyl, cyclohexyl, phenyl, benzyl, phenylethyl, thienylmethyl, piperidinyl, pyrrolodinyl, —CH₂Sphenyl and —CH(R₃)N(R₄)(R₅)
- wherein:
- R₃is chosen from
- H, phenyl, C_1-6alkyl, benzyl and phenylethyl;
- R₄is chosen from
- H, C_1-6alkyl, C_3-12cycloalkyl, C_3-7cycloalkylmethyl, benzyl, thienylethyl, and tetrahydropyran-4-ylmethyl;
- and R₅is chosen from
- H and methyl;
- or R₄and R₅together with the nitrogen atom they are connected to may form a piperidine group;
- wherein each cyclopentyl, cyclohexyl, phenyl, benzyl, phenylethyl, thienylmethyl, piperidinyl and pyrrolidinyl group is optionally substituted with 1-3 groups selected from halogen, C_1-6alkoxy, C_1-6alkyl, —CN, —NO₂, —OH—CF₃, —OCF₃, phenyl and amino optionally substituted by one or two C_1-6alkyl groups or a C(O)C_1-6alkyl group;
- wherein each phenyl group is optionally substituted with 1-3 groups selected from
- halogen, C_1-6alkoxy, C_1-6alkyl, —CN, —NO₂, —OH, —CF₃, —OCF₃, and amino optionally substituted by one or two C_1-6alkyl groups or a C(O)C_1-6alkyl group;
- R₂is chosen from H, Br and Cl;
- R₁₀is H;
- X is chosen from C and N; and
- or a tautomer thereof or a salt thereof, preferably a pharmaceutically acceptable salt thereof.

In still a further embodiment of the invention, there are provided compounds of the formula (I) selected from the group below or a tautomer thereof or a salt thereof, preferably a pharmaceutically acceptable salt thereof:


Structure	Name

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-dimethylamino-2- phenyl-acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-(4-methyl- piperazin-1-yl)- acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-(cyclohexylmethyl- amino)-acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-(4-hydroxy- piperidin-1-yl)-2-phenyl- acetamide

	2-Benzylamino-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-phenyl-acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-thiomorpholin-4- yl-propionamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-(1,2,3,4-tetrahydro- naphthalen-1-ylamino)- acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-(2-thiophen-2-yl- ethylamino)- propionamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-methylamino-2- phenyl-acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-cyclopropylamino- 2-phenyl-acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-(ethyl-methyl- amino)-2-phenyl- acetamide

	(S)-2-Amino-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-3-phenyl- propionamide

	(R)-2-Amino-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-3-phenyl- propionamide

	(R)-2-Amino-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-phenyl-acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-cyclobutylamino- 2-phenyl-acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2- (cyclopropylmethyl- amino)-2-phenyl- acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-(3-ethoxy- propylamino)-2-phenyl- acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-[(2- dimethylamino-ethyl)- ethyl-amino]-2-phenyl- acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-[(tetrahydro-pyran- 4-ylmethyl)-amino]- acetamide

	2-(Adamantan-1- ylamino)-N-(7-chloro-1- oxo-1,2-dihydro- isoquinolin-6-yl)- acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-[(pyridin-2- ylmethyl)-amino]- acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-[(pyridin-3- ylmethyl)-amino]- acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-[(pyridin-4- ylmethyl)-amino]- acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-(cyclohexylmethyl- amino)-propionamide

	(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-carbamic acid isopropyl ester

	2-Dimethylamino-N-(1- oxo-1,2-dihydro- isoquinolin-6-yl)- acetamide

	2-(Cyclohexylmethyl- amino)-N-(1-oxo-1,2- dihydro-isoquinolin-6- yl)-acetamide

	2-Dimethylamino-N-(1- oxo-1,2-dihydro- isoquinolin-6-yl)-2- phenyl-acetamide

	Acetic acid (7-chloro-1- oxo-1,2-dihydro- isoquinolin-6- ylcarbamoyl)-phenyl- methyl ester

	1-Benzyl-3-(1-oxo-1,2- dihydro-isoquinolin-6- yl)-urea

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-hydroxy-2-phenyl- acetamide

	(R)-2-Amino-N-(1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-2-phenyl- acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-(4-chloro-phenyl)- 3-methyl-butyramide

	2,5-Dichloro-thiophene- 3-carboxylic acid (1- oxo-1,2-dihydro- isoquinolin-6-yl)-amide

	N-(1-Oxo-1,2-dihydro- isoquinolin-6-yl)-2- phenylsulfanyl- nicotinamide

	2-(3-Methoxy-phenyl)- N-(1-oxo-1,2-dihydro- isoquinolin-6-yl)- acetamide

	2-(4-Chloro-phenoxy)- N-(1-oxo-1,2-dihydro- isoquinolin-6-yl)- nicotinamide

	2-(4-Chloro-phenoxy)-2- methyl-N-(1-oxo-1,2- dihydro-isoquinolin-6- yl)-propionamide

	N-(1-Oxo-1,2-dihydro- isoquinolin-6-yl)- succinamic acid ethyl ester

	Thiophene-2-carboxylic acid (1-oxo-1,2-dihydro- isoquinolin-6-yl)-amide

	1-(4-Chloro-phenyl)- cyclopentanecarboxylic acid (1-oxo-1,2-dihydro- isoquinolin-6-yl)-amide

	2-(4-Chloro-phenoxy)- N-(1-oxo-1,2-dihydro- isoquinolin-6-yl)- acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-methyl-benzamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-phenyl-acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2,3,6-trifluoro- benzamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-5-fluoro-2-methyl- benzamide

	2-Chloro-N-(7-chloro-1- oxo-1,2-dihydro- isoquinolin-6-yl)-4- nitro-benzamide

	2-Bromo-N-(7-chloro-1- oxo-1,2-dihydro- isoquinolin-6-yl)- benzamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-(4-fluoro-phenyl)- acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2,2-dimethyl- propionamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-3,3-dimethyl- butyramide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-phenylsulfanyl- nicotinamide

	2,4-Dichloro-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-benzamide

	5-Methyl-3-phenyl- isoxazole-4-carboxylic acid (7-chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-amide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2,4,6-trifluoro- benzamide

	2,3-Dichloro-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-benzamide

	1-(4-Chloro-phenyl)- cyclopentanecarboxylic acid (4-oxo-3,4-dihydro- quinazolin-7-yl)-amide

	Piperidine-4-carboxylic acid (4-oxo-3,4-dihydro- quinazolin-7-yl)-amide

	2-Benzylamino-N-(4- oxo-3,4-dihydro- quinazolin-7-yl)- acetamide

	1-Benzyl-piperidine-4- carboxylic acid (4-oxo- 3,4-dihydro-quinazolin- 7-yl)-amide

	Piperidine-3-carboxylic acid (4-oxo-3,4-dihydro- quinazolin-7-yl)-amide

	Pyrrolidine-2-carboxylic acid (4-oxo-3,4-dihydro- quinazolin-7-yl)-amide

	2-Amino-4-methyl- pentanoic acid (4-oxo- 3,4-dihydro-quinazolin- 7-yl)-amide

	(R)-2-Amino-N-(4-oxo- 3,4-dihydro-quinazolin- 7-yl)-3-phenyl- propionamide

	(S)-2-Amino-N-(4-oxo- 3,4-dihydro-quinazolin- 7-yl)-3-phenyl- propionamide

	2-(Cyclohexylmethyl- amino)-N-(4-oxo-3,4- dihydro-isoquinolin-7- yl)-acetamide

	2-Methylamino-N-(4- oxo-3,4-dihydro- quinazolin-7-yl)-2- phenyl-acetamide

	2-Amino-N-(4-oxo-3,4- dihydro-quinazolin-7- yl)-2-phenyl-acetamide

	2-Cyclopropylamino-N- (4-oxo-3,4-dihydro- quinazolin-7-yl)-2- phenyl-acetamide

	(R)-2-Amino-N-(4-oxo- 3,4-dihydro-quinazolin- 7-yl)-2-phenyl- acetamide

	(R)-Pyrrolidine-2- carboxylic acid (4-oxo- 3,4-dihydro-quinazolin- 7-yl)-amide

	(R)-2-Amino-3-methyl- N-(4-oxo-3,4-dihydro- quinazolin-7-yl)- butyramide

	2-(Cyclopropylmethyl- amino)-N-(4-oxo-3,4- dihydro-quinazolin-7- yl)-acetamide

	N-(4-Oxo-3,4-dihydro- quinazolin-7-yl)-2-(2- thiophen-2-yl- ethylamino)-acetamide

	2-(Cyclohexyl-methyl- amino)-N-(4-oxo-3,4- dihydro-quinazolin-7- yl)-acetamide

	N-(6-Chloro-4-oxo-3,4- dihydro-quinazolin-7- yl)-2-dimethylamino-2- phenyl-acetamide

	N-(6-Chloro-4-oxo-3,4- dihydro-quinazolin-7- yl)-2-cyclopropylamino- 2-phenyl-acetamide

	2,5-Dimethyl-2H- pyrazole-3-carboxylic acid (4-oxo-3,4-dihydro- quinazolin-7-yl)-amide

	2-Amino-N-(7-chloro-1- oxo-1,2-dihydro- isoquinolin-6-yl)-3-(4- fluoro-phenyl)- propionamide

	2-Amino-N-(7-chloro-1- oxo-1,2-dihydro- isoquinolin-6-yl)-2-(3- chloro-phenyl)-acetamide

	(R)-2-Amino-2- cyclohexyl-N-(1-oxo-1,2- dihydro-isoquinolin-6- yl)-acetamide

	(R)-2-Amino-2-(4- chloro-phenyl)-N-(1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-acetamide

	(R)-Pyrrolidine-2- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(S)-Pyrrolidine-2- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	2-Amino-N-(7-chloro-1- oxo-1,2-dihydro- isoquinolin-6-yl)- acetamide

	Piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide,

	(R)-2-Amino-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-3-(4-chloro-phenyl)- propionamide

	(R)-2-Amino-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-p-tolyl-acetamide

	(S)-2-Amino-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-3-cyclohexyl- propionamide

	(R)-2-Amino-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-3-cyclohexyl- propionamide

	(R)-2-Amino-4,4- dimethyl-pentanoic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(S)-2-Amino-4,4- dimethyl-pentanoic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	2-Chloro-N-(7-chloro-1- oxo-1,2-dihydro- isoquinolin-6-yl)-4- methanesulfonyl- benzamide

	2-Chloro-N-(7-chloro-1- oxo-1,2-dihydro- isoquinolin-6-yl)-4- morpholin-4-yl- benzamide

	(R)-2-Amino-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-3-naphthalen-2-yl- propionamide

	(R)-2-Amino-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-3-naphthalen-1-yl- propionamide

	(R)-2-Amino-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-3-pyridin-4-yl- propionamide

	2-Methyl-2- methylamino-N-(1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-propionamide

	2-Amino-2-methyl- N-(1- oxo-1,2-dihydro- isoquinolin-6-yl)- propionamide

	(R)-N-(7-Chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-2-methoxy-2- phenoxy-acetamide

	(S)-N-(7-Chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-2-methoxy-2- phenyl-acetamide

	3-Amino-N-(7-chloro-1- oxo-1,2-dihydro- isoquinolin-6-yl)- propionamide

	(R)-2-Amino-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-3-methyl-butyramide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-3,3,3-trifluoro-2- methoxy-2-phenyl- propionamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-oxo-2-phenyl- acetamide

	(R)-Piperidine-2- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	2-tert-Butylamino-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-acetamide

	(R)-2-Methoxy-N-(7- methoxy-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-phenyl-acetamide

	(S)-2-Methoxy-N-(7- methoxy-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-phenyl-acetamide

	(R)-2-Amino-2- cyclohexyl-N-(7- methoxy-1-oxo-1,2- dihydro-isoquinolin-6- yl)-acetamide

	(S)-2-Amino-2- cyclohexyl-N-(7- methoxy-1-oxo-1,2- dihydro-isoquinolin-6- yl)-acetamide

	4-Bromo-2-chloro-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-benzamide

	2-Chloro-N-(7-chloro-1- oxo-1,2-dihydro- isoquinolin-6-yl)-6- methanesulfonyl- benzamide

	(R)-Tetrahydro-furan-2- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(S)-N-(7-Chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-2-hydroxy-2- phenyl-acetamide

	(R)-N-(7-Chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-2-hydroxy-2- phenyl-acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-cyano-benzamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-methyl-4-nitro- benzamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-4-nitro-2- trifluoromethyl- benzamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-morpholin-4- ylmethyl-benzamide

	1-Amino- cyclohexanecarboxylic acid (1-oxo-1,2-dihydro- isoquinolin-6-yl)-amide

	(R)-2-Amino-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-cyclohexyl- acetamide

	(R)-2-Amino-4-methyl- pentanoic acid (7-chloro- 1-oxo-1,2-dihydro- isoquinolin-6-yl)-amide

	(S)-2-Amino-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-cyclohexyl- acetamide

	(S)-2-Amino-4-methyl- pentanoic acid (7-chloro- 1-oxo-1,2-dihydro- isoquinolin-6-yl)-amide

	(S)-2-Amino-N-(7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-3-methyl-butyramide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-(4-methyl- piperazin-1-ylmethyl)- benzamide

	1,2,3,4-Tetrahydro- isoquinoline-5-carboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	1,2,3,4-Tetrahydro- isoquinoline-8-carboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	1-Methyl-piperidine-4- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(R)-N-(7-Chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-3-methyl- succinamic acid methyl ester

	2-Chloro-N-(7-chloro-1- oxo-1,2-dihydro- isoquinolin-6-yl)-3-nitro- benzamide

	2-Chloro-N-(7-chloro-1- oxo-1,2-dihydro- isoquinolin-6-yl)-5-nitro- benzamide

	(S)-Tetrahydro-furan-2- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-3-methylsulfanyl- propionamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-cyano-acetamide

	2-Bromo-N-(7-chloro-1- oxo-1,2-dihydro- isoquinolin-6-yl)-4- methyl-benzamide

	Tetrahydro-pyran-4- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-3-phenyl-succinamic acid

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-malonamic acid ethyl ester

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-methylsulfanyl- acetamide

	(S)-Pyrrolidine-3- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(S)-Piperidine-3- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(R)-Piperidine-3- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-methanesulfonyl- acetamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-phenyl-malonamic acid

	(S)-Piperidine-2- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	1-Methanesulfonyl-4- methyl-piperidine-4- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	2-Chloro-N-(7-chloro-1- oxo-1,2-dihydro- isoquinolin-6-yl)-4-nitro- benzamide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-4-methanesulfonyl- benzamide

	(R)-Pyrrolidine-3- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	4-Amino- cyclohexanecarboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	4-Amino- cyclohexanecarboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	4-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- ylcarbamoyl)- cyclohexanecarboxylic acid

	4-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- ylcarbamoyl)- cyclohexanecarboxylic acid

	(R)-N-(7-Chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-2-isopropoxy-2- phenyl-acetamide

	(R)-N-(7-Chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-2-phenoxy- propionamide

	(S)-N-(7-Chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-2-phenoxy- propionamide

	4-Phenyl-piperidine-4- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	4-(4-Chloro-phenyl)- piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	4-Benzyl-piperidine-4- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(R)-Piperidine-3- carboxylic acid (1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-amide

	(S)-Piperidine-3- carboxylic acid (1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-amide

	1-Methyl-piperidine-4- carboxylic acid (1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-amide

	(R)-N-(7-Chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-2-hydroxy-3- methyl-butyramide

	Piperazine-2-carboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	4-Methyl-piperidine-4- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(3S,4S)-3-Methyl- piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(trans)-4-Phenyl- piperidine-3-carboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	Acetic acid 4-(7-chloro- 1-oxo-1,2-dihydro- isoquinolin-6- ylcarbamoyl)-cyclohexyl ester

	(1R,3S)-3-Amino- cyclopentanecarboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	4-Hydroxy- cyclohexanecarboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(S)-N-(7-Chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-2-cyclohexyl-2- hydroxy-acetamide

	(R)-N-(7-Chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-2-cyclohexyl-2- hydroxy-acetamide

	(S)-N-(7-Chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-2-hydroxy-2- phenyl-propionamide

	(R)-N-(7-Chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-2-hydroxy-2- phenyl-propionamide

	(1R,3S)-Amino- cyclohexanecarboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(1R,3S)-3-Amino- cyclohexanecarboxylic acid (7-methoxy-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-amide

	(3R,4S)-3-Methyl- piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	4-Dimethylamino- cyclohexanecarboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	3-Amino- cyclobutanecarboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	3-Amino- cyclobutanecarboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	4-Amino-1-(4-chloro- phenyl)- cyclohexanecarboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	4-Amino-1-(4-chloro- phenyl)- cyclohexanecarboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-4-methanesulfonyl-2- methyl-benzamide

	(1S,3S)-3-Amino- cyclohexanecarboxylic acid (7-chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-amide

	(S)-N-(7-Chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-2-cyclohexyl-2- isopropylamino- acetamide

	4-(4-Fluoro-phenyl)- pyrrolidine-3-carboxylic acid (1-oxo-1,2-dihydro- isoquinolin-6-yl)-amide

	(1S,2S)-2-Methyl-4-oxo- cyclohexanecarboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	3-Phenyl-pyrrolidine-3- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	1-Isopropyl-piperidine-4- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	4-Phenyl-pyrrolidine-3- carboxylic acid (1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-amide

	1-Cyclohexyl-piperidine- 4-carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	1-Pyridin-4-ylmethyl- piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-methyl-4- (piperazine-1-sulfonyl)- benzamide

	(R)-N-(7-Chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-2-(2-chloro- phenyl)-2-hydroxy- acetamide

	(R)-N-(7-Chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-2-(3-chloro- phenyl)-2-hydroxy- acetamide

	(2S,3R)-2-Amino-3- methyl-pentanoic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	N-(7-Chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-2-phenyl- isobutyramide

	1-Benzyl-piperidine-4- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(trans)-4-Phenyl- pyrrolidine-3-carboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(trans)-4-(4-Fluoro- phenyl)-pyrrolidine-3- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(3R,4S)-1,3-Dimethyl- piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	5-Phenyl-piperidine-3- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	1,2,3,4-Tetrahydro- isoquinolin-7-carboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	1,2,3,4-Tetrahydro- isoquinolin-6-carboxylic acid (7-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	4-(3-Amino-propane-1- sulfonyl)-2-chloro-N-(7- dihydro-isoquinolin-6- yl)-benzamide

	(trans)-4-(3-Bromo- phenyl)-pyrrolidine-3- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(trans)-4-(4-Chloro- phenyl)pyrrolidine-3- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide,

	(1R,5S,6R)-3-Aza- bicyclo[3.1.10]hexane-6- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(1R,5S,6S)-3-Aza- bicyclo[3.1.0]hexane-6- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(trans)-4-(3-Bromo- phenyl)-pyrrolidine-3- carboxylic acid (6- chloro-4-oxo-3,4- dihydro-quinazolin-7-yl)- amide

	(R)-N-(7-Chloro-1-oxo- 1,2-dihydro-isoquinolin- 6-yl)-2-(4-chloro- phenyl)-2-hydroxy- propionamide

	(R)-N-(6-Chloro-4-oxo- 3,4-dihydro-quinazolin- 7-yl)-2-(4-chloro- phenyl)-2-hydroxy- propionamide

	(R)-2-(4-Chloro-phenyl)- 2-hydroxy-N-(4-oxo-3,4- dihydro-quinazolin-7-yl)- propionamide

	1-Methyl-piperidine-4- carboxylic acid (7- bromo-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	1-Methyl-piperidine-4- carboxylic acid (7-fluoro- 1-oxo-1,2-dihydro- isoquinolin-6-yl)-amide

	1-Methyl-piperidine-4- carboxylic acid (7- chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	1-Methyl-piperidine-4- carboxylic acid (7-cyano- 1-oxo-1,2-dihydro- isoquinolin-6-yl)-amide

	(trans)-4-Phenyl- pyrrolidine-3-carboxylic acid (7-bromo-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(trans)-4-(4-Chloro- phenyl)-pyrrolidine-3- carboxylic acid (7- bromo-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide,

	(trans)-4-Phenyl- pyrrolidine-3-carboxylic acid (5-chloro-1-oxo-1,2- dihydro-isoquinolin-6- yl)-amide

	(trans)-4-Phenyl- pyrrolidine-3-carboxylic acid (7-chloro-5-fluoro- 1-oxo-1,2-dihydro- isoquinolin-6-yl)-amide

In an additional embodiment of the invention, there are provided compounds of the formula (I) selected from the group below or a tautomer thereof or a salt thereof, preferably a pharmaceutically acceptable salt thereof:

N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-dimethylamino-2-phenyl-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2(4-methyl-piperazin-1yl)-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(cyclohexylmethyl-amino)-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2(4-hydroxy-piperidin-1yl)-2-phenyl-acetamide;
2-Benzylamino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-phenyl-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-thiomorpholin-4-yl-propionamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(1,2,3,4-tetrahydro-naphthalen-1-ylamino)-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(2-thiophen-2-yl-ethylamino)-propionamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-methylamino-2-phenyl-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-cyclopropylamino-2-phenyl-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-ethyl-methyl-amino)-2-phenyl-acetamide;
(S)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-phenyl-propionamide;
(R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-phenyl-propionamide;
(R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-phenyl-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-cyclobutylamino-2-phenyl-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-cyclopropylmethyl-amino)-2-phenyl-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(3-ethoxy-propylamino)-2-phenyl-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-[(2-dimethylamino-ethyl)-ethyl-amino]-2-phenyl-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-[(tetrahydro-pyran-4-ylmethyl)-amino]-acetamide;
2-(Adamantan-1-ylamino)-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-[(pyridin-2-ylmethyl)-amino]-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-[(pyridin-3-ylmethyl)-amino]-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-[(pyridin-4-ylmethyl)-amino]-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(cyclohexylmethyl-amino)-propionamide;
(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-carbamic acid isopropyl ester;
2-Dimethylamino-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-acetamide;
2-(Cyclohexylmethyl-amino)-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-acetamide;
2-Dimethylamino-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-phenyl-acetamide;
Acetic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-ylcarbamoyl)-phenyl-methyl ester;
1-Benzyl-3-(1-oxo-1,2-dihydro-isoquinolin-6yl)-urea;
N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-hydroxy-2-phenyl-acetamide;
(R)-2-Amino-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-phenyl-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(4-chloro-phenyl)-3-methyl-butyramide;
2,5-Dichloro-thiophene-3-carboxylic acid (1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
N-(1-Oxo-1,2-dihydro-isoquinolin-6-yl)-2-phenylsulfanyl-nicotinamide;
2-(3-Methoxy-phenyl)-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-acetamide;
2-(4-Chloro-phenoxy)-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-nicotinamide;
2-(4-Chloro-phenoxy)-2-methyl-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-propionamide;
N-(1-Oxo-1,2-dihydro-isoquinolin-6-yl)-succinamic acid ethyl ester;
Thiophene-2-carboxylic acid (1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
1-(4-Chloro-phenyl)-cyclopentanecarboxylic acid (1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
2-(4-Chloro-phenoxy)-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-methyl-benzamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-phenyl-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2,3,6-trifluoro-benzamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-5-fluoro-2-methyl-benzamide
2-Chloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-4-nitro-benzamide;
2-Bromo-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-benzamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(4-fluoro-phenyl)-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2,2-dimethyl-propionamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3,3-dimethyl-butyramide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-phenylsulfanyl-nicotinamide;
2,4-Dichloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-benzamide;
5-Methyl-3-phenyl-isoxazole-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-yl)-2,4,6-trifluoro-benzamide;
2,3-Dichloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-benzamide;
2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-(4-fluoro-phenyl)-propionamide;
2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(3-chloro-phenyl)-acetamide;
(R)-2-Amino-2-cyclohexyl-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-acetamide;
(R)-2-Amino-2-(4-chloro-phenyl)-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-acetamide;
(R)-Pyrrolidine-2-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(S)-Pyrrolidine-2-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-acetamide;
Piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-(4-chloro-phenyl)-propionamide;
(R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-p-tolyl-acetamide;
(S)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-cyclohexyl-propionamide;
(R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-cyclohexyl-propionamide;
(R)-2-Amino-4,4-dimethyl-pentanoic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(S)-2-Amino-4,4-dimethyl-pentanoic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
2-Chloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-4-methanesulfonyl-benzamide;
2-Chloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-4-morpholin-4-yl-benzamide;
(R)-2-Amino-N-(7chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-naphthalen-2-yl-propionamide;
(R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-naphthalen-1-yl-propionamide;
(R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-pyridin-4yl-propionamide;
2-Methyl-2-methylamino-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-propionamide;
2-Amino-2-methyl-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-propionamide;
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-methoxy-2-phenyl-acetamide;
(S)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-methoxy-2-phenyl-acetamide;
3-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-propionamide;
(R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-methyl-butyramide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3,3,3-trifluoro-2-methoxy-2-phenyl-propionamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-oxo-2-phenyl-acetamide;
(R)-Piperidine-2-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
2-tert-Butylamino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-acetamide;
(R)-2-Methoxy-N-(7-methoxy-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-phenyl-acetamide;
(S)-2-Methoxy-N-(7-methoxy-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-phenyl-acetamide;
(R)-2-Amino-2-cyclohexyl-N-(7-methoxy-1-oxo-1,2-dihydro-isoquinolin-6-yl)-acetamide;
(S)-2-Amino-2-cyclohexyl-N-(7-methoxy-1-oxo-1,2-dihydro-isoquinolin-6-yl)-acetamide;
4-Bromo-2-chloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-benzamide;
2-Chloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-5-methanesulfonyl-benzamide;
(R)-Tetrahydro-furan-2-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(S)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-hydroxy-2-phenyl-acetamide;
(R)-N-(7Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-hydroxy-2-phenyl-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-cyano-benzamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-methyl-4-nitro-benzamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-4-nitro-2-trifluoromethyl-benzamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-morpholin-4-ylmethyl-benzamide;
1-Amino-cyclohexanecarboxylic acid (1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
- (R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-cyclohexyl-acetamide;
(R)-2-Amino-4-methyl-pentanoic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(S)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-cyclohexyl-acetamide;
(S)-2-Amino-4-methyl-pentanoic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(S)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-methyl-butyramide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(4-methyl-piperazin-1-ylmethyl)-benzamide;
1,2,3,4-Tetrahydro-isoquinoline-5-carboxylic acid (7-chloro-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
1,2,3,4-Tetrahydro-isoquinoline-8-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
1-Methyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-methyl-succinamic acid methyl ester;
2-Chloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-nitro-benzamide;
2-Chloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-5-nitro-benzamide;
(S)-Tetrahydro-furan-2-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-methylsulfanyl-propionamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-cyano-acetamide;
2-Bromo-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-4-methyl-benzamide;
Tetrahydro-pyran-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-phenyl-succinamic acid;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-malonamic acid ethyl ester;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-methylsulfanyl-acetamide;
(S)-Pyrrolidine-3-carboxylic acid(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(S)-Piperidine-3-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(R)-Piperidine-3-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-methanesulfonyl-acetamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-phenyl-malonamic acid;
(S)-Piperidine-2-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
1-Methanesulfonyl-4-methyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
2-Chloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-4-nitro-benzamide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-4-nitro-benzamide;
(R)-Pyrrolidine-3-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
4-Amino-cyclohexanecarboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
4-Amino-cyclohexanecarboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
4-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-ylcarbamoyl)-cyclohexanecarboxylic acid;
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-isopropoxy-2-phenyl-acetamide;
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-phenoxy-propionamide;
(S)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-phenoxy-propionamide;
4-Phenyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
4-(4-Chloro-phenyl)-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
4-Benzyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(R)-Piperidine-3-carboxylic acid (1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(S)-Piperidine-3-carboxylic acid (1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
1-Methyl-piperidine-4-carboxylic acid (1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-hydroxy-3-methyl-butyramide;
Piperazine-2-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
4-Methyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(3S,4S-Methyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(3R,4S)-4-Phenyl-piperidine-3-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
Acetic acid 4-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-ylcarbamoyl)-cyclohexyl ester (1R,3S)-3-Amino-cyclopentanecarboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
4-Hydroxy-cyclohexanecarboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(S)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-cyclohexyl-2-hydroxy-acetamide;
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-cyclohexyl-2-hydroxy-acetamide;
(S)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-hydroxy-2-phenyl-propionamide;
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-hydroxy-2-phenyl-propionamide;
(1R,3S)-3-Amino-cyclohexanecarboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(1R,3S)-3-Amino-cyclohexanecarboxylic acid (7-methoxy-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(3R,4S)-3-Methyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
4-Dimethylamino-cyclohexanecarboxylic acid (7-chloro-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
3-Amino-cyclobutanecarboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
4-Amino-1-(4-chloro-phenyl)-cyclohexanecarboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6yl)-4-methanesulfonyl-2-methyl-benzamide;
(1S,3S)-3-Amino-cyclohexanecarboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(S)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-cyclohexyl-2-isopropylamino-acetamide;
4-(4-Fluoro-phenyl)-pyrrolidine-3-carboxylic acid (1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(1S,2S)-2-Methyl-4-oxo-cyclohexanecarboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
3-Phenyl-pyrrolidine-3-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
1-Isopropyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
4-Phenyl-pyrrolidine-3-carboxylic acid (1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
1-Cyclohexyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
1-Pyridin-4-ylmethyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-methyl-4(piperazine-1-sulfonyl)-benzamide;
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(2-chloro-phenyl)-2-hydroxy-acetamide;
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(3-chloro-phenyl)-2-hydroxy-acetamide;
(2S,3R)-2-Amino-3-methyl-pentanoic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-phenyl-isobutyramide;
1-Benzyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(3R,4S)-4-Phenyl-pyrrolidine-3-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(3R,4S)-4-(4-Fluoro-phenyl)-pyrrolidine-3-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(3R,4S)-1,3-Dimethyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
5-Phenyl-piperidine-3-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
1,2,3,4-Tetrahydro-isoquinolin-7-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
1,2,3,4-Tetrahydro-isoquinolin-6-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
4-(3-Amino-propane-1-sulfonyl)-2-chloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-benzamide;
4-(3-Bromo-phenyl)-pyrrolidine-3-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
4-(4-chloro-phenyl)-pyrrolidine-3-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(1R,5S,6R)-3-Aza-bicyclo[3.1.0]hexane-6-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(1R,5S,6S)-3-Aza-bicyclo[3.1.0]hexane-6-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(4-chloro-phenyl)-2-hydroxy-propionamide;
1-Methyl-piperidine-4-carboxylic acid (7-bromo-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
1-Methyl-piperidine-4-carboxylic acid (7-fluoro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
1-Methyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
1-Methyl-piperidine-4-carboxylic acid (7-cyano-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide;
4-Phenyl-pyrrolidine-3-carboxylic acid (7-bromo-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide; and
4-(4-Chloro-phenyl)-pyrrolidine-3-carboxylic acid (7-bromo-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide.

In another embodiment of the invention, there are provided compounds of the formula (I) selected from the group below or a tautomer thereof or a salt thereof, preferably a pharmaceutically acceptable salt thereof:

1-(4-Chloro-phenyl)-cyclopentanecarboxylic acid (4-oxo-3,4-dihydro-quinazolin-7-yl)-amide;
Piperidine-4-carboxylic acid (4-oxo-3,4-dihydro-quinazolin-7-yl)-amide;
2-Benzylamino-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-acetamide;
1-Benzyl-piperidine-4-carboxylic acid (4-oxo-3,4-dihydro-quinazolin-7-yl)-amide;
Piperidine-3-carboxylic acid (4-oxo-3,4-dihydro-quinazolin-7-yl)-amide;
Pyrrolidine-2-carboxylic acid (4-oxo-3,4-dihydro-quinazolin-7-yl)-amide;
2-Amino-4-methyl-pentanoic acid (4-oxo-3,4-dihydro-quinazolin-7-yl)-amide;
(R)-2-Amino-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-3-phenyl-propionamide;
(S)-2-Amino-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-3-phenyl-propionamide;
2-(Cyclohexylmethyl-amino)-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-acetamide;
2-Methylamino-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-2-phenyl-acetamide;
2-Amino-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-2-phenyl-acetamide;
2-Cyclopropylamino-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-2-phenyl-acetamide;
(R)-2-Amino-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-2-phenyl-acetamide;
(R)-Pyrrolidine-2-carboxylic acid (4-oxo-3,4-dihydro-quinazolin-7-yl)-amide;
(R)-2-Amino-3-methyl-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-butyramide;
2-(Cyclopropylmethyl-amino)-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-acetamide;
N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-2-(2-thiophen-2-yl-ethylamino)-acetamide;
2-(Cyclohexyl-methyl-amino)-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-acetamide;
N-(6-Chloro-4-oxo-3,4-dihydro-quinazolin-7-yl)-2-dimethylamino-2-phenyl-acetamide;
N-(6-Chloro-4-oxo-3,4-dihydro-quinazolin-7-yl)-2-cyclopropylamino-2-phenyl-acetamide;
2,5-Dimethyl-2H-pyrazole-3-carboxylic acid (4-oxo-3,4-dihydro-quinazolin-7-yl)-amide;
4-(3-Bromo-phenyl)-pyrrolidine-3-carboxylic acid (6-chloro-4-oxo-3,4-dihydro-quinazolin-7-yl)-amide;
(R)-N-(6-Chloro-4-oxo-3,4-dihydro-quinazolin-7yl)-2-(4-chloro-phenyl)-2-hydroxy-propionamide; and
(R)-2-(4-Chloro-phenyl)-2-hydroxy-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-propionamide;

For the compounds disclosed hereinabove in this application, in the event the nomenclature is in conflict with the structure, it shall be understood that the compound is defined by the structure.
In another embodiment of the invention the above described compounds of formula (I) are used in methods of treating a disease-state or condition mediated by Rho kinase in an individual, the method comprising administering to the individual an effective amount of a compound of formula (I) or tautomer thereof, or salt thereof, preferably a pharmaceutically acceptable salt thereof.
In another embodiment of the invention the above described compounds of formula (I) are used in methods of treating a cardiovascular disease or condition in an individual, the method comprising administering to the individual an effective amount of a compound of formula (I) or tautomer thereof, or salt thereof, preferably a pharmaceutically acceptable salt thereof. Preferred cardiovascular disease or conditions are hypertension, atherosclerosis, restenosis, stroke, heart failure, coronary vasospasm, cerebral vasospasm, ischemia/reperfusion injury, pulmonary hypertension, angina, myocardial infarction, peripheral artery disease, coronary artery disease and combinations thereof.
In yet another embodiment of the invention the above described compounds of formula (I) are used in methods of treating renal disease, erectile dysfunction, asthma, glaucoma, or organ failure resulting from hypertension in an individual, the method comprising administering to the individual an effective amount of a compound of formula (I) or tautomer thereof, or salt thereof, preferably a pharmaceutically acceptable salt thereof.
Any compounds of this invention containing one or more asymmetric carbon atoms may occur as racemates and racemic mixtures, single enantiomers, diastereomeric mixtures and individual diastereomers. All such isomeric forms of these compounds are expressly included in the present invention. Each stereogenic carbon may be in the R or S configuration, or a combination of configurations.
Some of the compounds of formula (I) can exist in more than one tautomeric form. The invention includes methods using all such tautomers.
The compounds of the invention are meant to embrace compounds of Formula (I) as herein described, including the prodrugs, and the solvates and hydrates thereof.
All terms as used herein in this specification, unless otherwise stated, shall be understood in their ordinary meaning as known in the art. For example, “C_1-4alkyl” is a saturated aliphatic hydrocarbon monovalent radical containing 1-4 carbons such as methyl, ethyl, n-propyl, 1-methylethyl (isopropyl), n-butyl or t-butyl; “C_1-4alkoxy” is a C_1-4alkyl with a terminal oxygen, such as methoxy, ethoxy, propoxy, butoxy. All alkyl, alkenyl and alkynyl groups shall be understood as being branched or unbranched, cyclized or uncyclized where structurally possible and unless otherwise specified. Other more specific definitions are as follows:
Carbocycles including Cycloalkyl and Cycloalkenyl groups, are hydrocarbon rings containing from three to twelve carbon atoms. These carbocycles may be either aromatic or non-aromatic ring systems, monocyclic or polycyclic. The non-aromatic ring systems may be mono- or polyunsaturated. Preferred carbocycles include but are not limited to cyclopropyl, cyclobutyl, cyclopentyl, cyclopentenyl, cyclohexyl, cyclohexenyl, cycloheptanyl, cycloheptenyl, phenyl, indanyl, indenyl, benzocyclobutanyl, dihydronaphthyl, tetrahydronaphthyl, naphthyl, decahydronaphthyl, benzocycloheptanyl and benzocycloheptenyl. Certain terms for cycloalkyl such as cyclobutanyl and cyclobutyl shall be used interchangeably.
The term “heterocycle” refers to a stable nonaromatic 4-8 membered (but preferably, 5 or 6 membered) monocyclic or nonaromatic 8-11 membered bicyclic heterocycle radical which may be either saturated or unsaturated. Each heterocycle consists of carbon atoms and one or more, preferably from 1 to 4 heteroatoms chosen from nitrogen, oxygen and sulfur. The heterocycle may be attached by any atom of the cycle, which results in the creation of a stable structure. Unless otherwise stated, heterocycles include but are not limited to, for example pyrrolidinyl, pyrrolinyl, morpholinyl, thiomorpholinyl, thiomorpholinyl sulfoxide, thiomorpholinyl sulfone, dioxalanyl, piperidinyl, piperazinyl, tetrahydrofuranyl, tetrahydropyranyl, tetrahydrofuranyl, 1,3-dioxolanone, 1,3-dioxanone, 1,4-dioxanyl, piperidinonyl, tetrahydropyrimidonyl, pentamethylene sulfide, pentamethylene sulfoxide, pentamethylene sulfone, tetramethylene sulfide, tetramethylene sulfoxide and tetramethylene sulfone.
The term “heteroaryl” shall be understood to mean an aromatic 5-8 membered monocyclic or 8-11 membered bicyclic ring containing 1-4 heteroatoms such as N, O and S. Unless otherwise stated, such heteroaryls include thienyl, furanyl, isoxazolyl, oxazolyl, thiazolyl, thiadiazolyl, tetrazolyl, pyrazolyl, pyrrolyl, imidazolyl, pyridinyl, pyrimidinyl, pyrazinyl, pyridazinyl, pyranyl, quinoxalinyl, indolyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, benzothienyl, quinolinyl, quinazolinyl, naphthyridinyl, indazolyl, triazolyl, pyrazolo[3,4-b]pyrimidinyl, purinyl, pyrrolo[2,3-b]pyridinyl, pyrazolo[3,4-b]pyridinyl, tubercidinyl, oxazo[4,5-b]pyridinyl and imidazo[4,5-b]pyridinyl.
The term “heteroatom” used herein shall be understood to mean atoms other than carbon such as O, N, S and P.
In all alkyl groups or carbon chains one or more carbon atoms can be optionally replaced by heteroatoms: O, S or N, it shall be understood that if N is not substituted then it is NH, it shall also be understood that the heteroatoms may replace either terminal carbon atoms or internal carbon atoms within a branched or unbranched carbon chain. Such groups can be substituted as herein above described by groups such as oxo to result in definitions such as but not limited to: alkoxycarbonyl, acyl, amido and thioxo.
The term “aryl” as used herein shall be understood to mean aromatic carbocycle, such as phenyl or naphthyl or heteroaryl as defined above. Each aryl or heteroaryl unless otherwise specified includes it's partially or fully hydrogenated derivative. For example, quinolinyl may include decahydroquinolinyl and tetrahydroquinolinyl, naphthyl may include it's hydrogenated derivatives such as tetrahydranaphthyl. Other partially or fully hydrogenated derivatives of the aryl and heteroaryl compounds described herein will be apparent to one of ordinary skill in the art.
As used herein, “nitrogen” and “sulfur” include any oxidized form of nitrogen and sulfur and the quaternized form of any basic nitrogen. For example, for an —S—C_1-6alkyl radical, unless otherwise specified, this shall be understood to include —S(O)—C_1-6alkyl and —S(O)₂—C_1-6alkyl, likewise, —S—R_amay be represented as phenyl-S(O)_m— when R_ais phenyl and where m is 0, 1 or 2.
The term “halogen” as used in the present specification shall be understood to mean bromine, chlorine, fluorine or iodine, preferably fluorine or chlorine. The definitions “partially or fully halogenated”; partially or fully fluorinated; “substituted by one or more halogen atoms”, includes for example, mono, di or tri halo derivatives on one or more carbon atoms. For alkyl, a nonlimiting example would be —CH₂CHF₂, —CF₃etc.
The compounds of the invention ate only those which are contemplated to be ‘chemically stable’ as will be appreciated by those skilled in the art. For example, a compound which would have a ‘dangling valency’, or a ‘carbanion’ are not compounds contemplated by the inventive methods disclosed herein.
In another aspect of the invention, the compounds of the invention are formulated into pharmaceutical compositions comprising an effective amount, preferably a pharmaceutically effective amount of a compound of the invention or a tautomer, or salt thereof and a pharmaceutically acceptable excipient or carrier. Pharmaceutically acceptable salts are preferred.
The invention also provides a kit for the in vitro diagnostic determination of Rho kinase function in a sample, comprising: (a) a diagnostically effective amount of a compound according to the invention or a tautomer thereof, or salt thereof; and (b) instructions for use of the diagnostic kit.
The invention includes pharmaceutically acceptable derivatives of compounds of formula (I). A “pharmaceutically acceptable derivative” refers to any pharmaceutically acceptable salt or ester, or any other compound which, upon administration to an individual, is capable of providing (directly or indirectly) a compound useful for the invention, or a pharmacologically active metabolite or pharmacologically active residue thereof. A pharmacologically active metabolite shall be understood to mean any compound of the invention capable of being metabolized enzymatically or chemically. This includes, for example, hydroxylated or oxidized derivative compounds of the formula (I).
Pharmaceutically acceptable salts include those derived from pharmaceutically acceptable inorganic and organic acids and bases. Examples of suitable acids include hydrochloric, hydrobromic, sulfuric, nitric, perchloric, fumaric, maleic, phosphoric, glycolic, lactic, salicylic, succinic, toluene-p-sulfonic, tartaric, acetic, citric, methanesulfonic, formic, benzoic, malonic, naphthalene-2-sulfonic and benzenesulfonic acids. Other acids, such as oxalic acid, while not themselves pharmaceutically acceptable, may be employed in the preparation of salts useful as intermediates in obtaining the compounds and their pharmaceutically acceptable acid addition salts. Salts derived from appropriate bases include alkali metal (e.g., sodium), alkaline earth metal (e.g., magnesium), ammonium and N—(C₁-C₄alkyl)₄ ⁺ salts.
In addition, within the scope of the invention is use of prodrugs of compounds of the formula (I). Prodrugs include those compounds that, upon simple chemical transformation, are modified to produce compounds of the invention. Simple chemical transformations include hydrolysis, oxidation and reduction. Specifically, when a prodrug is administered to an individual, the prodrug may be transformed into a compound disclosed hereinabove, thereby imparting the desired pharmacological effect.

General Synthetic Methods

The invention additionally provides for methods for making the compounds of the formula (I). The compounds of the invention may be prepared by the general methods and examples presented below, and methods known to those of ordinary skill in the art. Optimum reaction conditions and reaction times may vary depending on the particular reactants used. Unless otherwise specified, solvents, temperatures, pressures, and other reaction conditions may be readily selected by one of ordinary skill in the art. Specific procedures are provided in the Synthetic Examples section. Reaction progress may be monitored by conventional methods such as thin layer chromatography (TLC). Intermediates and products may be purified by methods known in the art, including column chromatography, HPLC or recrystallization.
The isoquinolinone core structures used to prepare compounds of formula (I) having X═C may be prepared by methods known in the art (for example. F. Eloy and A. Deryckere, J. Heterocyclic Chem, 1971, 8, 57; N. Briet et al., Tetrahedron, 2002, 58, 5761) and outlined in Scheme 1.
A nitro substituted cinnamic acid (II) is reduced to the corresponding amine using for example tin II chloride in hydrochloric acid. The amine is protected with a protecting group compatible with the cyclization conditions, such as an acetyl group. The resulting amide substituted cinnamic acid (III) is converted to the corresponding acyl azide by methods known in the art, for example, activation of the carboxylic acid by treatment with a chloroformate such as ethyl chloroformate, in the presence of an amine such as triethylamine, followed by treatment of the resulting mixed anhydride with an aqueous solution of an azide such as sodium azide. The resulting acyl azide (IV) is converted to the isoquinolone by heating in a solvent such as an ether of diethylene glycol, such as diethylene glycol dibutyl ether (dibutyl carbitol) or diphenyl ether, preferably in the presence of a base such as tributyl amine, at a temperature of 200-300° C., preferably 230-260° C. In some cases, depending on the nature of R₁, a mixture of regioisomeric products is formed, (Va and Vb) which can be separated by chromatography.
The protecting group may be removed from the separated 6-amino isomer by hydrolyis, preferably under acidic conditions using an acid such as concentrated hydrochloric acid. The resulting amino isoquinolinone formed (VI) can be used to prepare amide derivatives of formula (I) (X═C, Y═—NHC(O)—) by coupling with a carboxylic acid or carboxylic acid derivative using methods well known in the art and described in the Synthetic Examples section below. Ureas (X═C, Y═—NHC(O)NH—) and carbamates (X═C, Y═—NHC(O)O—) may be prepared by reaction of the isoquinolinome VI with an isocyanate or chloroformate respectively.
Quinazolinone intermediates (IX) used to prepare compounds of formula (I) having X═N may be prepared by methods known in the art as described in Scheme 2.
Heating the nitro anthranilic acid (VII) with a salt of formamidine, preferably the hydrochloride or acetate salt, at a temperature of about 200° C., provides VIII (Q. Chao et al., J. Med. Chem. 1999, 42, 3860). Alternatively, the anthranilic acid may be heated with formamide at a temperature of 130 to 150° C. in a microwave reactor (F.-R. Alexandre et al., Tet. Lett. 2002, 43, 3911, and F.-R. Alexandre et al., Tet. Lett. 2003, 44, 4455) to provide VIII. Reduction of the nitro group to the amine (IX) may be carried out by standard methods, such as catalytic hydrogenation over palladium, or transfer hydrogenation using a palladium catalyst and ammonium formate as the hydrogen source. The resulting amino quinazolinone formed (IX) can be used to prepare amide derivatives of formula (I) (X═N, Y═—NHC(O)—) by coupling with a carboxylic acid or carboxylic acid derivative) using methods well known in the art and described in the Synthetic Examples section below. Ureas (X═N, Y═—NHC(O)NH—) and carbamates (X═C, Y═—NHC(O)O—) may be prepared by reaction of the isoquinolinome VI with an isocyanate or chloroformate respectively.

SYNTHETIC EXAMPLES

Examples 1-7 illustrate the synthesis of 6-aminoisoquinolin-1-one and 7-aminoquinazolin-4-one intermediates that may be used to prepare desired compounds of formula (I).

Example 1

Synthesis of 6-aminoisoquinolin-1-one

Tin II chloride dihydrate (1.70 g, 0.75 mol) was dissolved in concentrated HCl (200 mL). Warming was necessary to obtain a clear solution. 3-Nitrocinnamic acid (35 g, 0.181 mol) was added portionwise with stirring. After about 20% of the 3-nitrocinnamic acid had been added, the mixture was warmed with a heat gun to 45° C. to initiate reaction. The rate of addition was controlled to maintain a temperature of 65-75° C. The reaction was exothermic, but not vigorous on this scale. After the addition was complete, and the reaction started to subside, the flask was transferred to an oil bath at 65° C. for 1 h. The mixture was then cooled to room temperature. The solid was filtered, washed with 2 N HCl, (100 mL) and sucked dry. The filter cake was dried in a vacuum oven for 1 h, then in air overnight, to give about 65 g of the crude amine. This material was suspended in acetic acid (300 mL) and acetic anhydride (150 mL, 1.6 mol) was added gradually with stirring. After the initial exotherm subsided, the mixture was stirred in an oil bath at 110° C. for 2 h, resulting in a clear solution. LCMS showed complete conversion to the desired acetanilide. The solution was cooled and water (50 mL) was added. After standing overnight the solution was concentrated to about 300 mL. More water (100 mL) and 2 N HCl (50 mL) were added. The precipitate was filtered, washed with water and dried to give 3-acetamido cinnamic acid (33.2 g, 89%).
A solution of 3-acetamido cinnamic acid (3.0 g, 15 mmol) and triethylamine (4.2 mL, 30 mmol) in acetone (27 mL) was cooled in an ice bath. Ethyl chloroformate (1.86 mL, 19.5 mmol) was added, and the solution was stirred in ice for 40 min. Triethylamine hydrochloride precipitated. A solution of sodium azide (1.46 g, 22.5 mmol) in water (3 mL) was added. The mixture was stirred in ice for 40 min, then for 1 h at room temperature. Water (15 mL) was added, and the solution was extracted three times with dichloromethane. The combined organic layers were dried over magnesium sulfate and evaporated to dryness to give the crystalline azide.
While this reaction was in progress, a 100 mL 3-neck round bottom flask, equipped with magnetic stirrer, pressure-equalizing addition funnel, thermocouple connected to a J-KEM controller and an air condenser topped by short-path distillation head and receiver, was charged with a mixture of diethyleneglycol dibutyl ether (dibutyl carbitol) (10 mL) and tributyl amine (3.3 mL, 15 mmol). The stirred solution was heated to 240° C. using a heating mantle connected to the J-KEM controller. Nitrogen was passed through the apparatus via an inlet connected to the top of the addition funnel. The azide was redissolved in dichloromethane (80 mL, with warming), and this solution was placed in the addition funnel. The nitrogen stream was stopped. The azide solution was added slowly dropwise, to maintain the temperature between 230° C. and 240° C. Very vigorous gas evolution occurred as the azide was added. The dichloromethane boiled off as the solution was added. The addition took 2 h 10 min, during which time a distillate collected in the receiver, which was a mixture of dichloromethane and dibutyl carbitol. Periodically during the addition, the receiver was changed. The dichloromethane was evaporated, and the liquid remaining in the flask was returned to the reaction vessel. After the addition was complete, the temperature was maintained at 240° C. for 40 min. The healing mantle was removed. A dark solid had deposited on the walls of the flask. On cooling, a crystalline precipitate formed. The precipitate was collected by filtration, and washed with ether. The dark solid remained in the flask. More solid crystallized from the ether filtrate on standing. The supernatant was decanted, and the solid combined with the filtered precipitate. The supernatant was distilled under high vacuum, to remove the carbitol and tributylamine. The combined solids and pot residue from the distillation contained both the 8-acetamido isoquinolin-1-one and 6-acetamido isoquinolin-1-one. This material was purified by chromatography on silica, using a dichloromethane/methanol gradient from 2% to 15%. The first eluted component was the 8-acetamido isomer (700 mg isolated pure). The second eluted component was the desired 6-acetamido isomer (600 mg). This component contained impurities, which were removed by triturating the material with a little methanol (ca. 3 mL). The crystals were filtered, washed with a few drops of methanol and dried to give pure 6-acetamido isoquinolin-1-one (354 mg, 12%).
A stirred suspension of 6-acetamidoisoquinolin-1-one (1.62 g, 8.0 mmol) in 6 M HCl (60 mL) was healed in an oil bath at 65° C. with stirring. After 2 h a clear solution was obtained, and the reaction was complete by LCMS. The solution was cooled and evaporated almost to dryness. Methanol was evaporated from residue to leave the hydrochloride salt as a crystalline solid. The salt was suspended in water (40 mL) and heated to at 60° C. to dissolve. To the resulting solution at 60° C., ammonium hydroxide was added dropwise, immediately forming a precipitate. The mixture was cooled in ice, the crystals filtered, and washed with water, and finally with a few drops of methanol, which removed some brown color. The crystals were dried to give the title compound (1.00 g, 78%).

Example 2

Synthesis of 6-amino-5-chloroisoquinolin-1-one

Tin II chloride dihydrate (103 g, 0.46 mol) was dissolved in concentrated HCl (120 mL). Warming was necessary to obtain a clear solution. 4-Chloro-3-nitrocinnamic acid (25 g, 0.11 mol) was added portionwise with stirring. After about 20% of the starting material had been added, the mixture was warmed with a heat gun to 45° C. to initiate reaction. The rate of addition was controlled to maintain a temperature of 65-75° C. Cooling with a water bath was used to prevent the temperature exceeding 80° C. After the addition was complete and reaction started to subside, the mixture was transferred to an oil bath at 65° C. for 1 h. The mixture was cooled to room temperature. The solid was filtered, washed with 2 M HCl, (60 mL) and sucked dry. The filter cake was dried in a vacuum oven for 1 h, then in air overnight, to give about 34 g crude product. This material was suspended in acetic acid (100 ml) with stirring and acetic anhydride (100 mL, 1.1 mol) was added in one portion. The temperature rose to 35° C. After the initial exotherm subsided, the mixture was stirred in an oil bath at 110° C. for 2 h, but did not dissolve. LCMS showed complete conversion to the desired acetanilide. The solution was cooled and water (50 mL) was added cautiously. The mixture was concentrated to about 200 mL. More water (100 mL) and 2 M HCl (50 mL) were added. The precipitate was filtered, washed with water and dried to give 3-acetamido-4-chlorocinnamic acid (25.0 g, 95%).
A suspension of 3-acetamido-4-chlorocinnamic acid (7.19 g, 30 mmol) and triethylamine (8.4 mL, 60 mmol) in acetone (100 mL) was cooled in an ice bath. Ethyl chloroformate (3.73 mL, 39 mmol) was added, and the solution was stirred in ice for 40 min. The acid dissolved and a precipitate formed. A solution of sodium azide (2.93 g, 45 mmol) in water (10 mL) was added. The mixture was stirred in ice for 40 min, then for 1 h at room temperature. Water (75 mL) was added, and the solution was extracted three times with dichloromethane. The combined organic layers were dried over magnesium sulfate and evaporated to dryness, to give the crystalline azide.
While this reaction was in progress, the apparatus described above in Example 1 was assembled, using a 250 mL 3-neck round bottom flask, which was charged with diethyleneglycol dibutyl ether (dibutyl carbitol) (75 mL) and tributyl amine (7.2 mL, 30 mmol). The stirred solution was heated to 240° C. using a heating mantle connected to the J-KEM controller. Nitrogen was passed through the apparatus via an inlet connected to the top of the addition funnel. The azide was redissolved in dichloromethane (160 mL), with warming, and this solution was placed in the addition funnel. The nitrogen stream was stopped. The azide solution was added dropwise, to maintain temp between 230° C. and 250° C., so that the dichloromethane boiled off as the solution was added. The addition took 1 h 40 min. A distillate collected in the receiver, which was a mixture of dichloromethane and dibutyl carbitol. Periodically during the addition, the receiver was changed. The dichloromethane was evaporated, and the liquid remaining in the flask was returned to the reaction vessel. After the addition was complete, the temperature was maintained at 240° C. for 30 min. The heating mantle was removed. On cooling, a crystalline precipitate formed. The cooled solution was diluted with ether (150 mL) and stirred overnight. The precipitate was collected by filtration, washed well with ether, then with a few drops of MeOH, which removed some dark color. The resulting off-white solid was dried at 60° C. under vacuum to give 6-acetamido-5-chloroisoquinoline-1-one (3.1 g). This material contained a minor component which was not removed at this stage.
A stirred suspension of 6-acetamido-5-chloroisoquinolin-1-one (1.4 g, 5.9 mmol) in concentrated HCl (50 mL) was heated in an oil bath at 65° C. with stirring. After 1 h, a clear solution was obtained, and the reaction was complete by LCMS. The solution was cooled and evaporated almost to dryness. Methanol was evaporated from the residue to leave the hydrochloride salt as a crystalline solid. The salt was dissolved in methanol and treated with excess ammonium hydroxide to form the free base. The solution was evaporated to dryness and was purified by chromatography on a Combiflash system, using a dichloromethane/methanol gradient from 2% to 10%. The major, second eluting, spot was collected to yield the title compound (883 mg, 72%).
Analogous procedures were used to make the 7-H, 7-bromo, 7-fluoro, and 7-trifluoromethyl derivatives using commercially available starting materials. The 7-cyano and 7-methoxy analogs were made by the following methods below.

Example 3

Synthesis of 6-amino-7-cyano isoquinolin-1-one

A reaction vial was charged with 7-bromo-6-aminoisoquinolone hydrochloride (203 mg, 0.74 mmol), Zn(CN)₂(155 mmol) and Pd(Ph₃P)₄(0.37 mmol) in 1.5 mL of dry dimethylacetamide. The vial was sealed and warmed to 100° C. for 20 minutes in a microwave. The reaction was recharged with additional Pd(Ph₃P)₄(0.37 mmol) and warmed for another 20 minutes. The reaction was then diluted with 1 N aqueous NaOH and the resulting solids were isolated by filtration (615 mg). The desired product was present in both the solids as well as the filtrate. The solids were purified by suspension in dimethylacetamide, filtration to remove insoluble material and reverse phase HPLC chromatography of the filtrate to afford the desired product as a white solid (62 mg, 46%). The aqueous filtrate from above was neutralized with 1 N aqueous HCl and the resulting crude product was isolated by filtration (233 mg). This additional material was used without further purification.

Example 4

Synthesis of 6-Amino-7-methoxy isoquinolin-1-one

To a solution of 3-methoxy-4-nitrobenzoic acid (10.00 g, 50.72 mmol) in CH₂Cl₂(250 mL) was added pyridine (4.89 mL, 60.87 mmol) followed by SOCl₂(4.06 mL, 55.80 mmol). The reaction was stirred for 5 min then volatiles were removed in vacuo. The crude was dissolved in CH₂Cl₂(200 mL) and a solution of triethylamine (8.72 mL, 60.87 mmol) and aminoacetaldehyde dimethyl acetal (6.40 g, 60.87 mmol) in CH₂Cl₂(50 mL) was added via syringe. The mixture was stirred for 5 min then diluted with CH₂Cl₂(250 mL), washed with saturated aqueous NaHCO₃(2×200 mL), dried with MgSO₄, filtered, and concentrated to afford the crude product (14.0 g) which was used as is in the next transformation.
A solution of the above nitro compound (14.0 g) and 20% Pd(OH)₂on carbon (1.8 g) was dissolved in MeOH (150 mL). A balloon filled with hydrogen was attached and the system was purged and evacuated (3×). The suspension was allowed to stir for 20 h then was filtered through a pad of diatomaceous earth. The filtrate was concentrated in vacuo to afford the desired aniline intermediate as a gold foam (12.25 g, 98%). MS (ES+) m/e 255 [M+H]⁺
A solution of the aniline (11.00 g, 43.26 mmol) in concentrated H₂SO₄(100 mL) was stirred at 100° C. for 1 h. The mixture was cooled to 0° C. and carefully treated with 6 M NaOH to pH=10. The aqueous mixture was extracted with EtOAc (5×300 mL), dried with MgSO₄, filtered, and concentrated to give a crude beige solid that was purified by suspending in CH₂Cl₂(50 mL) and filtering the solid. The solid was dried to give the desired 6-amino-7-methoxy-isoquinolin-1-one (1.55 g, 19%). MS (ES+) m/e 191 [M+H]⁺

Example 5

Synthesis of 6-amino-5-chloro isoquinolin-1-one

To a solution of 0.500 g (3.12 mmol) of 6-amino-2H-isoquinolin-1-one in DMF (30 mL) was added 0.45 g (3.4 mol) of N-chlorosuccinimide. The mixture was stirred at room temperature for 15 h then poured over ice and stirred until all the ice was melted during which time a solid precipitated from solution. The tan solid was collected by filtration, washed with water and dried on the filter pad to provide 0.50 g (82% yield) of the title compound. MS calc. for C₉H₈ClN₂O [M+H]⁺; 195.63. Found: 195.23.

Example 7

Synthesis or 6-amino-7-chloro-5-fluoro-isoquinolin-1-one

To 20.0 mL (215 mmol) of phosphorous oxychloride, cooled to 0 C, was added 5.00 g (25.7 mmol) of 6-amino-7-chloro-2H-isoquinolin-1-one in portions. The mixture was heated to 100° C. for 3 h then cooled to room temperature and poured over ice. After all of the ice had melted the pH of the mixture was adjusted to slightly alkaline by the slow addition of a 10% aqueous NaOH solution. A yellow solid precipitated from solution and was collected by filtration, washed with water and dried over anhydrous Na₂SO₄to provide 3.0 g (55%) of 1,7-dichloro-isoquinolin-6-ylamine. MS calc. for C₉H₇Cl₂N₂[M+H]⁺: 214.08. Found: 213.23 and 215.23.
To a solution of 2.62 g (12.3 mmol) of 1,7-dichloro-isoquinolin-6-ylamine in DMF (130 mL) was added 5.5 g (15.5 mmol) of Selectfluor® as a solid in one portion. The mixture was stirred at room temperature for 15 h. The mixture was poured over ice and stirred until all of the ice had melted during which time a solid precipitated from solution. The brown solid was collected by filtration, washed with water and dried on the filter pad to provide 1.0 g (60% by ¹H-NMR, 21%) of 1,7-dichloro-5-fluoro-isoquinolin-6-ylamine. MS calc. for C₉H₅Cl₂FN₂[M]⁺: 231.05. Found: 231.68.
A mixture of 1.0 g (4.3 mmol) of the above crude intermediate in 50 mL (100 mmol) of a 2 N solution of hydrochloric acid was heated at 100° C. for 15 h then cooled to room temperature. The pH of the solution was adjusted to slightly alkaline by the addition of a 10% NaOH solution. A solid precipitated from solution and was collected by filtration and dried on the filter pad. The residue was purified by flash silica gel chromatography using a 0-50% gradient of MeCN to hexanes to provide 0.15 g (16%) of the title compound. MS calc. for C₉H₇ClFN₂O [M+H]⁺: 213.62. Found: 213.42.

Example 8

Synthesis of 7-aminoquinazolin-4-one

4-Nitroanthranilic acid (10.0 g. 54.9 mmol) and formamidine hydrochloride (6.63 g, 82.4 mmol) were ground together in a mortar and pestle to produce a fine, intimate mixture. The mixture was placed in a 250 mL round-bottom flask, and spread evenly over the surface. The flask was placed in an oilbath at 200° C. The solid underwent a color change, and a distillate was seen on the side of flask, but did not really melt. After 30 min the flask was removed from the heating bath. 0.3M sodium hydroxide solution (150 mL) was added to the cooled flask, the black solid mass was broken up with a spatula, and stirred for 1 h. The solid was filtered off and washed with water. The filtrate was discarded. The black solid was suspended in dichloromethane/methanol (10:1) and filtered through a plug of silica, eluting with the same solvent until no more product came off. The material was one spot by TLC, plus black baseline material, but was poorly soluble, so a large volume of solvent was needed. The filtrate was evaporated to dryness and the solid residue triturated with a little methanol and filtered to give 7-nitroquinazolin-4-one (4.65 g, 44%).
A suspension of 7-nitroquinazolinone (5.2 g, 22.7 mmol) in DMF (150 mL) and methanol (100 mL) was hydrogenated over 10% palladium on carbon (600 mg) in a Parr shaker at 50 psi. The starting material was consumed in 3 h, but hydrogenation was continued for 18 h to ensure complete reaction. The mixture was filtered through diatomaceous earth, washing with methanol/DMF 2:1 until all of the product was eluted. The filtrate was evaporated to dryness. The solid residue was stirred with methanol (20 mL) for 1 h, filtered, washed with methanol and dried to give the crude amine (3.88 g), which contained a minor component (ca. 10%). The crude product was dissolved in 2 N HCl (100 mL), with warming, and the resulting solution was evaporated to dryness to give the hydrochloride salt. The salt was dissolved in boiling water (30 mL) and ethanol (30 mL) was added. The solvent was boiled down to 50 mL. The hydrochloride crystallized on cooling. The crystals were filtered, washed with a little ethanol and dried to give the pure salt (3.02 g). The salt was dissolved in water (30 mL) with warming to 65° C. in an oil bath. Ammonium hydroxide was added dropwise, causing immediate precipitation of the free base. The flask was cooled in ice, the crystals filtered, washed with water and dried to give the title compound (2.35 g, 54%).

Example 9

Synthesis of 6-Chloro-7-aminoquinazolin-4-one

Methanol (500 mL) and concentrated H₂SO₄(25 mL) were added to 4-nitroanthranilic acid (30.0 g, 165.0 mmol) and the reaction mixture was heated at reflux for 48 h. The resulting solution was concentrated and saturated NaHCO₃(200 mL) was added. The aqueous layer was extracted with EtOAc (2×150 mL). The combined organic extracts were washed with water (100 mL), and brine (100 mL), dried over Na₂SO₄, filtered, and concentrated to give the methyl ester (29.0 g, 90%) as an intense orange solid.
To a suspension of 4-nitroanthranilic acid methyl ester (16.5 g, 84 mmol) in glacial acetic acid (500 mL), a solution of sulfuryl chloride (13.6 g, 0.1 mol) in glacial acetic acid (20 mL) was added dropwise. The homogeneous mixture was stirred at room temperature for 18 h and the solvent was evaporated in vacuo. The residue was dissolved in CH₂Cl₂(200 mL) and washed with saturated NaHCO₃(100 mL) and water (100 mL). The organic layer was dried over Na₂SO₄, filtered, and concentrated. The ¹H NMR spectrum of the crude compound revealed the formation of a mixture of products, namely the desired 5-chloro regioisomer, and the byproducts 3-chloro regioisomer and the 3,5-dichlorinated compound. The crude mixture was purified by flush chromatography (silica gel, gradient 30-100% hexanes/CH₂Cl₂) to afford the desired 5-Chloro-4-nitroanthranilic acid methyl ester (7.1 g, 36%) as a pale yellow solid.
To a solution of 5-chloro-4-nitroanthranilic acid methyl ester (10.0 g, 43 mmol) in THF (120 mL) was added LiOH (2.7 g, 65 mmol) dissolved in water (40 mL). The reaction mixture was stirred at room temperature for 18 h and acidified to pH ˜4 with 1 N HCl. The aqueous layer was extracted with EtOAc (2×150 mL); the combined organic layers were dried over Na₂SO₄, filtered, and concentrated to give 5-chloro-4-nitroanthranilic acid (8.7 g, 88%) as an orange solid.
A mixture of 5-chloro-4-nitroanthranilic acid (8.7 g, 0.040 mol) and formamidine hydrochloride (4.9 g, 60 mmol) were ground to a fine, intimate powder using a mortar and pestle. The mixture was transferred into a 250 mL round bottomed flask and immersed in an oil bath maintained at 205° C. After 40 min, LCMS analysis of the reaction mixture showed mass corresponding to the product. The reaction mixture was cooled and the crude product was crushed into pieces and washed several times with saturated NaHCO₃(300 mL) and water (200 mL). The solid was air dried and triturated with a minimum amount of MeOH (20 mL) to obtain 6-chloro-7-nitroquinazolin-4-one (7.6 g, 83%) as a tan powder.
To a suspension of 6-chloro-7-nitroquinazolin-4-one (7.5 g, 33 mmol) in MeOH (250 mL) was added NH₄Cl (17.7 g, 0.33 mol) dissolved in water (75 mL). Iron powder (18.5 g, 330 mmol) was added and the suspension was heated at 65° C. for 2 h. The warm reaction mixture was filtered over diatomaceous earth and washed several times with THF (1 L) and methanol (500 mL). The filtrate was concentrated, washed with water and dried under vacuum at 50° C. to afford the title compound (6.5 g, 90%) as a brown solid. ESI-MS m/z 196 [C₈H₆ClN₃O+H]⁺.

Example 10

Parallel Synthesis of Amides of Formula (I)

Method 1

Intermediates from Examples 1-4 were acylated with a variety of acid chlorides as follows: Acid chlorides were dissolved in dimethylacetamide (DMA) to give a 0.67 mM solution. The amine intermediates were dissolved in DMA to give a 0.171 mM solution. 150 microL (100 micromol) of acid chloride solution was transferred to each well of a deep-well microtitre plate. 350 microL (60 micromol) of the appropriate amine solution was added to each well. The plate was sealed and placed on a shaker for 24 h. Silica-bound amine scavenger (about 50 mg) was added to each well, and the plate was shaken for 30 min. Silica-bound carbonate scavenger (about 125 mg) was added to each well, and the plates were shaken for a further 20 h. The contents of the plate were transferred to a filter plate and filtered into a fresh deep-well plate. The scavenger was washed with two 400 microL portions of DMA. Aliquots were removed from each well for LCMS analysis. The solutions were evaporated in a Genevac. The purity was assessed by UV at 240 nm. Samples below 80% purity were purified by preparative HPLC.

Example 11

Synthesis of N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-cyclopropylamino-2-phenyl-acetamide

Method 2

7-Chloro, 6-aminoisoquinolone (25 mg, 0.13 mmol) was dissolved in DMF. 2-Chlorophenylacetyl chloride (1.2 eq, 22 microL, 0.15 mmol) was added followed by diisopropylethylamine (2 eq., 45 microL, 0.26 mmol). The reaction mixture was shaken for 3 hr on a reaction block at 75° C. This crude mixture was carried on to the next step without purification.
The crude reaction mixture from the previous step was taken as is and excess cyclopropylamine (100 microL, 11 eq.) was added. The reaction mixture was shaken on a reaction block for 16 hr at 75° C. This crude reaction mixture was purified directly as is on preparative LC-MS. N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-cyclopropylamino-2-phenyl-acetamide (21 mg, 0.05 mmol) was isolated as an amorphous solid in 40% yield. ES+=368.

Example 12

Synthesis of 2-amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-phenyl-propionamide

Method 3

6-Amino-7-chloro-2H-isoquinolin-1-one (0.2 mmol, 39 mg) and L-Fmoc-2-amino-3-phenyl-propionyl chloride (0.2 mmol, 81 mg) were dissolved in 3 mL DMF. The mixture was allowed to stir at room overnight. HPLC-MS confirmed the product had formed. The crude product was carried on to the next step without further purification. MS (M+1) 565.
The above crude product in DMF was treated with piperidine (5-10% total concentration), and the reaction mixture was allowed to stir at room overnight. The title compound was obtained after prep-HPLC purification. MS (M+1) 342.

Example 13

Synthesis of 1-benzyl-3-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-urea

Method 4

6-Amino-2H-isoquinolin-1-one (0.2 mmol, 39 mg) and benzyl isocyanate (0.2 mmol, 27 mg) were dissolved in 1 mL DMA. The mixture was allowed to stir at 60° C. overnight. The product was obtained by HPLC purification. MS (M+1) 294.

Example 14

Synthesis of 4-(4-chloro-phenyl)-pyrrolidine-3-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide

To a solution of benzyl-methoxymethyl-trimethylsilanylmethyl-amine (2.05 mL, 8.0 mmol) and (E)-3-(4-chloro-phenyl)-acrylic acid methyl ester (1.57 g, 8.0 mmol) in dichloromethane (16 mL) was added TFA (275 mg, 2.4 mmol). The mixture was stirred at 23° C. for 30 min then treated with saturated aqueous NaHCO₃(20 mL), extracted with dichloromethane (2×50 mL), dried with MgSO₄, filtered, and concentrated. Purification of the crude by flash chromatography (SiO₂, hexane to 1:1 hexane:EtOAc) gave (trans)-1-benzyl-4-(4-chloro-phenyl)-pyrrolidine-3-carboxylic acid ethyl ester (2.11 g, 80%).
A solution of the ester from above (1.01 g, 2.94 mmol) in MeOH (15 mL) and THF (5 mL) was treated with a 6 M aqueous NaOH solution (4.9 mL, 29.4 mmol) and heated at reflux for 2 h. The mixture was then acidified to pH=1 with 6 M aqueous HCl, extracted with dichloromethane, dried with MgSO₄, filtered, and concentrated to afford 1-benzyl-4-(4-chloro-phenyl)-pyrrolidine-3-carboxylic acid as the HCl salt (1.00 g, 97%).
To a solution of the acid from above (1.06 g, 3.0 mmol) and 6-amino-5-chloroisoquinolin-1-one (450 mg, 2.3 mmol) in pyridine (5 mL) was added POCl₃(320 μL, 3.5 mmol). The mixture was stirred for 1 h then treated with water, extracted with EtOAc, dried with MgSO₄, filtered, and concentrated to afford 1-benzyl-4-(4-chloro-phenyl)-pyrrolidine-3-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide (454 mg, 40%).
The above N-benzyl pyrrolidine (216 mg, 0.44 mmol) was dissolved in dichloroethane (5 mL) and treated with alpha chloroethyl chloroformate (95 microL, 0.88 mmol) followed by proton sponge (69 mg, 0.44 mmol). The mixture was stirred at 23° C. for 1 h then heated at reflux for 3 h. The mixture was then concentrated in vacuo, diluted with MeOH (10 mL), and heated at reflux for 2 h. The solution was concentrated in vacuo then purified by RP HPLC to afford the desired product as the TFA salt (88 mg, 40%) MS MH+=402.3
The following compounds were also prepared using the methods described in the General Synthetic Methods section and the synthetic examples above:


Name	MH+

N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	357
dimethylamino-2-phenyl-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(4-methyl-	336
piperazin-1-yl)-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	349
(cyclohexylmethyl-amino)-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(4-	413
hydroxy-piperidin-1-yl)-2-phenyl-acetamide
2-Benzylamino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-	419
yl)-2-phenyl-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	353
thiomorpholin-4-yl-propionamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(1,2,3,4-	383
tetrahydro-naphthalen-1-ylamino)-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(2-	377
thiophen-2-yl-ethylamino)-propionamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	343
methylamino-2-phenyl-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(ethyl-	371
methyl-amino)-2-phenyl-acetamide
(S)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	343
3-phenyl-propionamide
(R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	343
3-phenyl-propionamide
(R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	329
2-phenyl-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	383
cyclobutylamino-2-phenyl-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	383
(cyclopropylmethyl-amino)-2-phenyl-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(3-ethoxy-	415
propylamnio)-2-phenyl-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-[(2-	428
dimethylamino-ethyl-amino]-2-phenyl-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	351
[(tetrahydro-pyran-4-ylmethyl)-amino]-acetamide
2-(Adamantan-1-ylamino)-N-(7-Chloro-1-oxo-1,2-dihydro-	387
isoquinolin-6-yl)-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-[(pyridin-2-	344
ylmethyl)-amino]-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-[(pyridin-3-	344
ylmethyl)-amino]-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-[(pyridin-4-	344
ylmethyl)-amino]-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	349
(cyclohexyl-methyl-amino)-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	281
methylamino-propionamide
2-Dimethylamino-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-	246
acetamide
2-(Cyclohexylmethyl-amino)-N-(1-oxo-1,2-dihydro-	314
isoquinolin-6-yl)-acetamide
2-Dimethylamino-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	322
phenyl-acetamide
Acetic acid (7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-	372
ylcarbamoyl)-phenyl-methyl ester
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-hydroxy-2-	330
phenyl-acetamide
(R)-2-Amino-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-phenyl-	294
acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(4-chloro-	390
phenyl)-3-methyl-butyramide
2,5-Dichloro-thiophene-3-carboxylic acid (1-oxo-1,2-dihydro-	340
isoquinolin-6-yl)-amide
N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-phenylsulfanyl-	374
nicotinamide
2-(3-Methoxy-phenyl)-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-	309
acetamide
2-(4-Chloro-phenoxy)-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-	393
nicotinamide
2-(4-Chloro-phenoxy)-2-methyl-N-(1-oxo-1,2-dihydro-	358
isoquinolin-6-yl)-propionamide
N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-succinamic acid ethyl	289
ester
Thiophene-2-carboxylic acid (1-oxo-1,2-dihydro-isoquinolin-6-	271
yl)-amide
1-(4-Chloro-phenyl)-cyclopentanecarboxylic acid (1-oxo-1,2-	368
dihydro-isoquinolin-6-yl)-amide
2-(4-Chloro-phenoxy)-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-	330
acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-methyl-	314
benzamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-phenyl-	314
acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2,3,6	354
trifluoro-benzamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-5-fluoro-2-	332
methyl-benzamide
2-Chloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-4-	379
nitro-benzamide
2-Bromo-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	379
benzamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(4-fluoro-	332
phenyl)-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2,2-dimethyl-	280
propionamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3,3-dimethyl-	294
butyramide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2	409
phenylsulfanyl-nicotinamide
2,4-Dichloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	369
benzamide
5-Methyl-3-phenyl-isoxazole-4-carboxylic acid (7-chloro-1-	381
oxo-1,2-dihydro-isoquinolin-6-yl)-amide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2,4,6-	354
trifluoro-benzamide
1-(4-Chloro-phenyl)-cyclopentanecarboxylic acid (4-oxo-3,4-	368
dihydro-quinazolin-7-yl)-amide
Piperidine-4-carboxylic acid (4-oxo-3,4-dihydro-quinazolin-7-	273
yl)-amide
2-benzylamino-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-	309
acetamide
1-benzyl-piperidine-4-carboxylic acid (4-oxo-3,4-dihydro-	363
quinazolin-7-yl)-amide
Piperidine-3-carboxylic acid (4-oxo-3,4-dihydro-quinazolin-7-	273
yl)-amide
Pyrrolidine-2-carboxylic acid (4-oxo-3,4-dihydro-quinazolin-7-	259
yl)-amide
2-Amino-4-methyl-pentanoic acid (4-oxo-3,4-dihydro-	275
quinazolin-7-yl)-amide
(R)-2-Amino-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-3-phenyl-	309
propionamide
(S)-2-Amino-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-3-phenyl-	309
propionamide
2-(Cyclohexylmethyl-amino)-N-(4-oxo-3,4-dihydro-quinazolin-	315
7-yl)-acetamide
2-Methylamino-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-2-	309
phenyl-acetamide
2-Amino-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-2-phenyl-	295
acetamide
2-Cyclopropylamino-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-2-	335
phenyl-acetamide
(R)-2-Amino-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-2-phenyl-	295
acetamide
(R)-Pyrrolidine-2-carboxylic acid (4-oxo-3,4-dihydro-	259
quinazolin-7-yl)-amide
(R)-2-Amino-3-methyl-N-(4-oxo-3,4-dihydro-quinazolin-7-yl)-	261
butyramide
2-(Cyclopropylmethyl-amino)-N-(4-oxo-3,4-dihydro-	273
quinazolin-7-yl)-acetamide
N-(4-Oxo-3,4-dihydro-quinazolin-7-yl)-2-(2-thiophen-2-yl-	329
ethylamino)-acetamide
2-(Cyclohexyl-methyl-amino)-N-(4-oxo-3,4-dihydro-	315
quinazolin-7-yl)-acetamide
N-(6-Chloro-4-oxo-3,4-dihydro-quinazolin-7-yl)-2-	357
dimethylamino-2-phenyl-acetamide
N-(6-Chloro-4-oxo-3,4-dihydro-quinazolin-7-yl)-2-	369
cyclopropylamino-2-phenyl-acetamide
2,5-Dimethyl-2H-pyrazole-3-carboxylic acid (4-oxo-3,4-	284
dihydro-quinazolin-7-yl)-amide
2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-(4-	360
fluoro-phenyl)-propionamide
(R)-2-Amino-2-cyclohexyl-N-(1-oxo-1,2-dihydro-isoquinolin-	300
6-yl)-acetamide
(R)-2-Amino-2-(4-chloro-phenyl)-N-(1-oxo-1,2-dihydro-	362
isoquinolin-6-yl)-acetamide
(R)-Pyrrolidine-2-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-	292
isoquinolin-6-yl)-amide
(S)-Pyrrolidine-2-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-	292
isoquinolin-6-yl)-amide
2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	252
acetamide
Piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-	306
isoquinolin-6-yl)-amide
(R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	376
3-(4-chloro-phenyl)-propionamide
(R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	342
2-p-tolyl-acetamide
(S)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	348
3-cyclohexyl-propionamide
(R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	348
3-cyclohexyl-propionamide
(R)-2-Amino-4,4-dimethyl-pentanoic acid (7-chloro-1-oxo-1,2-	322
dihydro-isoquinolin-6-yl)-amide
(S)-2-Amino-4,4-dimethyl-pentanoic acid (7-chloro-1-oxo-1,2-	322
dihydro-isoquinolin-6-yl)-amide
2-Chloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-4-	411
methanesulfonyl-benzamide
2-Chloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-4-	418
morpholin-4-yl-benzamide
(R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	392
3-naphthalen-2-yl-propionamide
(R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	392
3-naphthalen-1-yl-propionamide
(R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	343
3-pyridin-4-yl-propionamide
2-Methyl-2-methylamino-N-(1-oxo-1,2-dihydro-isoquinolin-6-	260
yl)-propionamide
2-Amino-2-methyl-N-(1-oxo-1,2-dihydro-isoquinolin-6-yl)-	246
propionamide
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	343
methoxy-2-phenyl-acetamide
(S)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	343
methoxy-2-phenyl-acetamide
3-Amino-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	266
propionamide
(R)-2-Amino-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	294
3-methyl-butyramide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3,3,3-	411
trifluoro-2-methoxy-2-phenyl-propionamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-oxo-2-	327
phenyl-acetamide
(R)-Piperidine-2-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-	306
isoquinolin-6-yl)-amide
2-tert-Butylamino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-	308
6-yl)-acetamide
(R)-2-Methoxy-N-(7-methoxy-1-oxo-1,2-dihydro-isoquinolin-	339
6-yl)-2-phenyl-acetamide
(S)-2-Methoxy-N-(7-methoxy-1-oxo-1,2-dihydro-isoquinolin-6-	339
yl)-2-phenyl-acetamide
(R)-2-Amino-2-cyclohexyl-N-(7-methoxy-1-oxo-1,2-dihydro-	330
isoquinolin-6-yl)-acetamide
(S)-2-Amino-2-cyclohexyl-N-(7-methoxy-1-oxo-1,2-dihydro-	330
isoquinolin-6-yl)-acetamide
4-Bromo-2-chloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-	413
6-yl)-benzamide
2-Chloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-5-	412
methanesulfonyl-benzamide
(R)-Tetrahydro-furan-2-carboxylic acid (7-chloro-1-oxo-1,2-	293
dihydro-isoquinolin-6-yl)-amide
(S)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	329
hydroxy-2-phenyl-acetamide
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	329
hydroxy-2-phenyl-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-cyano-	324
benzamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-methyl-4-	358
nitro-benzamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-nitro-4-	412
trifluoromethyl-benzamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-morpholin-	398
4-ylmethyl-benzamide
1-Amino-cyclohexanecarboxylic acid (1-oxo-1,2-dihydro-	286
isoquinolin-6-yl)-amide
(R)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	334
2-cyclohexyl-acetamide
(R)-2-Amino-4-methyl-pentanoic acid (7-chloro-1-oxo-1,2-	308
dihydro-isoquinolin-6-yl)-amide
(S)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	334
2-cyclohexyl-acetamide
(S)-2-Amino-4-methyl-pentanoic acid (7-chloro-1-oxo-1,2-	308
dihydro-isoquinolin-6-yl)-amide
(S)-2-Amino-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-	294
3-methyl-butyramide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(4-methyl-	411
piperazin-1-ylmethyl)-benzamide
1,2,3,4-Tetrahydro-isoquinoline-5-carboxylic acid (7-chloro-1-	354
oxo-1,2-dihydro-isoquinolin-6-yl)-amide
1,2,3,4-Tetrahydro-isoquinoline-8-carboxylic acid (7-chloro-1-	354
oxo-1,2-dihydro-isoquinolin-6-yl)-amide
1-Methyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-	320
dihydro-isoquinolin-6-yl)-amide
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-methyl-	323
succinamic acid methyl ester
2-Chloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-	378
nitro-benzamide
2-Chloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-5-	378
nitro-benzamide
(S)-Tetrahydro-furan-2-carboxylic acid (7-chloro-1-oxo-1,2-	293
dihyrdo-isoquinolin-6-yl)-amide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-	297
methylsulfanyl-propionamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-cyano-	262
acetamide
2-Bromo-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-4-	392
methyl-benzamide
Tetrahydro-pyran-4-carboxylic acid (7-chloro-1-oxo-1,2-	307
dihydro-isoquinolin-6-yl)-amide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-3-phenyl	371
succinamic acid
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-malonamic	309
acid ethyl ester
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	283
methylsulfanyl-acetamide
(S)-Pyrrolidine-3-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-	292
isoquinolin-6-yl)-amide
(S)-Piperidine-3-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-	306
isoquinolin-6-yl)-amide
(R)-Piperidine-3-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-	306
isoquinolin-6-yl)-amide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	315
methanesulfonyl-acetamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-phenyl	357
malonamic acid
(S)-Piperidine-2-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-	306
isoquinolin-6-yl)-amide
1-Methanesulfonyl-4-methyl-piperidine-4-carboxylic acid (7-	398
chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide
2-Chloro-N-(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-4-	378
nitro-benzamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-4-	377
methanesulfonyl-benzamide
(S)-Pyrrolidine-3-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-	292
isoquinolin-6-yl)-amide
4-Amino-cyclohexanecarboxylic acid (7-chloro-1-oxo-1,2-	320
dihydro-isoquinolin-6-yl)-amide
4-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-ylcarbamoyl)-	349
cyclohexanecarboxylic acid
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	371
isopropoxy-2-phenyl-acetamide
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	343
phenoxy-propionamide
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	343
phenoxy-propionamide
4-Phenyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-	382
dihydro-isoquinolin-6-yl)-amide
4-(4-Chloro-phenyl)-piperidine-4-carboxylic acid (7-chloro-1-	416
oxo-1,2-dihydro-isoquinolin-6-yl)-amide
4-Benzyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-	396
dihydro-isoquinolin-6-yl)-amide
(R)-Piperidine-3-carboxylic acid (1-oxo-1,2-dihydro-	272
isoquinolin-6-yl)-amide
(S)-Piperidine-3-carboxylic acid (1-oxo-1,2-dihydro-	272
isoquinolin-6-yl)-amide
1-Methyl-piperidine-4-carboxylic acid (1-oxo-1,2-dihydro-	286
isoquinolin-6-yl)-amide
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	295
hydroxy-3-methyl-butyramide
Piperidine-2-carboxylic acid (7-chloro-1-oxo-1,2-dihydro-	307
isoquinolin-6-yl)-amide
4-Methyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-	320
dihydro-isoquinolin-6-yl)-amide
(3S,4S)-3-Methyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-	306
1,2-dihydro-isoquinolin-6-yl)-amide
(trans)-4-Phenyl-piperidine-3-carboxylic acid (7-chloro-1-oxo-	382
1,2-dihydro-isoquinolin-6-yl)-amide
(1R,3S)-3-Amino-cyclopentanecarboxylic acid (7-chloro-1-oxo-	306
1,2-dihydro-isoquinolin-6-yl)-amide
4-Hydroxy-cyclohexanecarboxylic acid (7-chloro-1-oxo-1,2-	321
dihydro-isoquinolin-6-yl)-amide
(S)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	335
cyclohexyl-2-hydroxy-acetamide
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	335
cyclohexyl-2-hydroxy-acetamide
(S)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	343
hydroxy-2-phenyl-propionamide
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	343
hydroxy-2-phenyl-propionamide
(1R,3S)-3-Amino-cyclohexanecarboxylic acid (7-chloro-1-oxo-	320
1,2-dihydro-isoquinolin-6-yl)-amide
(1R,3S)-3-Amino-cyclohexanecarboxylic acid (7-methoxy-1-	316
oxo-1,2-dihydro-isoquinolin-6-yl)-amide
(3R,4S)-3-Methyl-piperidine-4-carboxylic acid (7-chloro-1-	320
oxo-1,2-dihydro-isoquinolin-6-yl)-amide
4-Dimethylamino-cyclohexanecarboxylic acide (7-chloro-1-oxo-	348
1,2-dihydro-isoquinolin-6-yl)-amide
Trans-3-amino-cyclobutanecarboxylic acid (7-chloro-1-oxo-1,2-	292
dihydro-isoquinolin-6-yl)-amide
Cis-3-amino-cyclobutanecarboxylic acid (7-chloro-1-oxo-1,2-	292
dihydro-isoquinolin-6-yl)-amide
4-Amino-1-(cis-4-chloro-phenyl)-cyclohexanecarboxylic acid	430
(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-amide
4-Amino-1-(trans-4-chloro-phenyl)-cyclohexanecarboxylic acid	430
(7-chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide
(S)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	391
cyclohexyl-2-isopropylamino-acetamide
(S)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	320
cyclohexyl-2-isopropylamino-acetamide
(S)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-	376
cyclohexyl-2-isopropylamino-acetamide
4-(4-Fluoro-phenyl)-pyrrolidine-3-carboxylic acid (1-oxo-1,2-	352
dihydro-isoquinolin-6-yl)-amide
(1S,2S)-2-Methyl-4-oxo-cyclohexanecarboxylic acid (7-chloro-	333
1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide
3-Phenyl-pyrrolidine-3-carboxylic acid (7-chloro-1-oxo-1,2-	368
dihydro-isoquinolin-6-yl)-amide
1-Isopropyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-	348
dihydro-isoquinolin-6-yl)-amide
1-Cyclohexyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-	334
dihydro-isoquinolin-6-yl)-amide
1-Cyclohexyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-	388
dihydro-isoquinolin-6-yl)-amide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-methyl-4-	397
(piperazine-1-sulfonyl)-benzamide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-methyl-4-	461
(piperazine-1-sulfonyl)-benzamide
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(2-	363
chloro-phenyl)-2-hydroxy-acetamide
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(3-	363
chloro-phenyl)-2-hydroxy-acetamide
(2S,3R)-2-Amino-3-methyl-pentanoic acid (7-chloro-1-oxo-1,2-	308
dihydro-isoquinolin-6-yl)-amide
N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-phenyl	341
isobutyramide
1-Benzyl-piperidine-4-carboxylic acid (7-chloro-1-oxo-1,2-	396
dihydro-isoquinolin-6-yl)-amide
(trans)-4-Phenyl-pyrrolidine-3-carboxylic acid (7-chloro-1-oxo-	368
1,2-dihydro-isoquinolin-6-yl)-amide
(trans)-4-(4-Fluoro-phenyl)-pyrrolidine-3-carboxylic acid (7-	386
chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide
(3R,4S)-1,3-Dimethyl-piperidine-4-carboxylic acid (7-chloro-1-	334
oxo-1,2-dihydro-isoquinolin-6-yl)-amide
5-Phenyl-piperidine-3-carboxylic acid (7-chloro-1-oxo-1,2-	382
dihydro-isoquinolin-6-yl)-amide
1,2,3,4-Tetrahydro-isoquinoline-7-carboxylic acid (7-chloro-1-	354
oxo-1,2-dihydro-isoquinolin-6-yl)-amide
1,2,3,4-Tetrahydro-isoquinoline-6-carboxylic acid (7-chloro-1-	354
oxo-1,2-dihydro-isoquinolin-6-yl)-amide
4-(3-Amino-propane-1-sulfonyl)-2-chloro-N-(7-chloro-1-oxo-	454
1,2-dihydro-isoquinolin-6-yl)-benzamide
(trans)-4-(3-Bromo-phenyl)-pyrrolidine-3-carboxylic acid (7-	447
chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide
(trans)-4-(4-Chloro-phenyl)-pyrrolidine-3-carboxylic acid (7-	402
chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide,
(1R,5S,6R)-3-Aza-bicyclo[3.1.0]hexane-6-carboxylic acid (7-	304
chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide
(1R,5S,6S)-3-Aza-bicyclo[3.1.0]hexane-6-carboxylic acid (7-	304
chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide
(trans)-4-(3-Bromo-phenyl)-pyrrolidine-3-carboxylic acid (6-	448
chloro-4-oxo-3,4-dihydro-quinazolin-7-yl)-amide
(R)-N-(7-Chloro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-2-(4-	377
chloro-phenyl)-2-hydroxy-propionamide
(R)-N-(6-Chloro-4-oxo-3,4-dihydro-quinazolin-7-yl)-2-(4-	378
chloro-phenyl)-2-hydroxy-propionamide
(R)-2-(4-Chloro-phenyl)-2-hydroxy-N-(4-oxo-3,4-dihydro-	344
quinazolin-7-yl)-propionamide
1-Methyl-piperidine-4-carboxylic acid (7-bromo-1-oxo-1,2-	365
dihydro-isoquinolin-6-yl)-amide
1-Methyl-piperidine-4-carboxylic acid (7-fluoro-1-oxo-1,2-	304
dihydro-isoquinolin-6-yl)-amide
1-Methyl-piperidine-4-carboxylic acid (7-cyano-1-oxo-1,2-	311
dihydro-isoquinolin-6-yl)-amide
(trans)-4-Phenyl-pyrrolidine-3-carboxylic acid (7-bromo-1-oxo-	413
1,2-dihydro-isoquinolin-6-yl)-amide
4-(4-Chloro phenyl)-pyrrolidine-3-carboxylic acid (7-bromo-1-	447
oxo-1,2-dihydro-isoquinolin-6-yl)-amide
(trans)-4-Phenyl-pyrrolidine-3-carboxylic acid (5-chloro-1-oxo-	369
1,2-dihydro-isoquinolin-6-yl)-amide
(trans)-4-Phenyl-pyrrolidine-3-carboxylic acid (7-chloro-5-	386
fluoro-1-oxo-1,2-dihydro-isoquinolin-6-yl)-amide

Assessment of Biological Activity

Molecular Assay

The activity of ROCKII (1-543) kinase was measured utilizing Cambrex PKLight ATP Detection Reagent, a homogeneous assay technology using luciferin-luciferase to quantify residual ATP. The assay was performed in 384-well low-volume, white, non-binding surface microtiter plates (Corning). The assay buffer was 25 mM HEPES, pH 7.5, 10 mM MgCl₂, 50 mM KCl, 0.2% BSA, 0.01% CHAPS, 100 μM Na₃VO₄and 0.5 mM DTT. Test compounds, dissolved in neat DMSO at 500 μg/mL, were serially diluted for dose response for a final starting concentration of 3 μg/mL in 1% DMSO of assay buffer. ROCKII (1-543) (62,408 Da) was diluted in assay buffer to a final concentration of 7.5 nM in a total volume of 15 μL. Positive controls were reaction mixtures containing no test compound; negative controls (blanks) were reaction mixtures containing no kinase. After 15 minutes of pre-incubation of the test compounds with the kinase, a mixture of ATP and peptide substrate (AKRRRLSSLRA) in assay buffer was added to each well for a final concentration of 750 nM ATP and 500 nM peptide, respectively. After 90 minutes of incubation of the kinase reaction at 28° C. temperature, 10 μL of PKLight ATP Detection Reagent (warmed to room temperature previously) was added to each well. The assay plate was incubated at room temperature for additional 15 minutes and then read on an Analyst in luminescence mode. Dose-response experiments of each test compounds were conducted in quadrulet. IC₅₀values of test compounds represent 50% response of the positive control from the dose-response curve.
Preferred compounds have an IC₅₀<1 μM in this assay.
Selected compounds were evaluated in a Rat Aortic Rings tissue assay:

Rat Aortic Rings Tissue Assay

Segments of rat thoracic aorta were dissected from Sprague Dawley rats cleaned of excess connective tissue, and cut into 3-4 mm rings in a petri dish filled with 4° C. PBS, containing 118 mM NaCl; 4.7 mM KCl; 1.6 mM CaCl₂; 1.2 mM KH2PO4; 1.2 mM MgCl₂; 10.0 mM Dextrose; 25 mM NaHCO3; 0.02 mM NaEDTA; pH 7.25, and kept on ice before dissection. The rings were then suspended on a force transducer device and placed into 37° C. temperature-controlled tissue baths containing PBS that was constantly oxygenated with 95% O₂and 5% CO₂. Isometric force was continually measured and the data collected by a digital acquisition system. The rings were placed under a preload of 2.5 g of force for a 1 hr equilibration period to serve as baseline force. Rings were contracted with 50 mM KCl to obtain the maximum contraction level for normalization. Following a washout period of 30 min, the rings were pre-constricted with 10⁻⁶M phenylephrine and relaxed with a bolus dose of 10⁻⁷M acetylcholine to check the integrity of the endothelium. Following a second washout period of 30 min, rings were pre-constricted a second time with 10⁻⁶M phenylephrine and the contraction allowed to stabilize. A cumulative dose response of a Rho-kinase inhibitor was tested in a DMSO vehicle at a 1:1000 dilution using half log intervals. After each dose of inhibitor the response was allowed to stabilize before the addition of the next dose. Following the cumulative dose response with inhibitor, the tissues were washed 3× in PBS and allowed to equilibrate at resting tension. A second KCl contraction was performed as stated above to check the viability of the tissue. Following this, a second phenylephrine contraction and acetylcholine bolus dose were given as above to cheek for the integrity of the endothelium following inhibitor testing. The effect of the Rho-kinase inhibitors were expressed as a percentage relaxation from the phenylephrine-induced contraction at each dose. The IC₅₀for each inhibitor was determined from the concentration that produced 50% relaxation from the phenylephrine-induced contraction. The data for each inhibitor represents the mean from four different segments from four different rats.
Preferred compounds have an IC₅₀<10 μM in this assay.

Methods of Therapeutic Use

In accordance with the invention, there are provided novel methods of using the compounds of the formula (I). The compounds disclosed therein effectively inhibit Rho kinase. The inhibition of Rho kinase is an attractive means for preventing and treating a variety of cardiovascular diseases or conditions associated with Rho kinase activation. Thus, the compounds are useful for the treatment of diseases and conditions as described in the Background section, including the following conditions and diseases; hypertension, atherosclerosis, restenosis, stroke, myocardial infarction, heart failure, coronary artery disease, peripheral artery disease, coronary vasospasm, cerebral vasospasm, ischemia/reperfusion injury, pulmonary hypertension, angina, erectile dysfunction, renal disease and organ failure. As disclosed in the Background section, the compounds of the invention will also be useful for treating diseases or conditions associated with smooth muscle hyper reactivity or with activated Rho-kinase under other pathophysiological conditions. These diseases include but are not limited to asthma, glaucoma, cancer, Alzheimer's disease, multiple sclerosis, spinal cord injury and neuropathic pain.
These disorders have been well characterized in man, but also exist with a similar etiology in other mammals, and can be treated by pharmaceutical compositions of the present invention.
For therapeutic use, the compounds of the invention may be administered via a pharmaceutical composition in any conventional pharmaceutical dosage form in any conventional manner. Conventional dosage forms typically include a pharmaceutically acceptable carrier suitable to the particular dosage form selected. Routes of administration include, but are not limited to, intravenously, intramuscularly, subcutaneously, intrasynovially, by infusion, sublingually, transdermally, orally, topically or by inhalation. The preferred modes of administration are oral and intravenous.
The compounds of this invention may be administered alone or in combination with adjuvants that enhance stability of the inhibitors, facilitate administration of pharmaceutical compositions containing them in certain embodiments, provide increased dissolution or dispersion, increase inhibitory activity, provide adjunct therapy, and the like, including other active ingredients. In one embodiment, for example, multiple compounds of the present invention can be administered. Advantageously, such combination therapies utilize lower dosages of the conventional therapeutics, thus avoiding possible toxicity and adverse side effects incurred when those agents are used as monotherapies. Compounds of the invention may be physically combined with the conventional therapeutics or other adjuvants into a single pharmaceutical composition. Advantageously, the compounds may then be administered together in a single dosage form. In some embodiments, the pharmaceutical compositions comprising such combinations of compounds contain at least about 5%, but more preferably at least about 20%, of a compound of formula (I) (w/w) or a combination thereof. The optimum percentage (w/w) of a compound of the invention may vary and is within the purview of those skilled in the art. Alternatively, the compounds of the present invention and the conventional therapeutics or other adjuvants may be administered separately (either serially or in parallel). Separate dosing allows for greater flexibility in the dosing regime.
As mentioned above, dosage forms of the compounds of this invention may include pharmaceutical acceptable carriers and adjuvants known to those of ordinary skill in the art and suitable to the dosage form. These carriers and adjuvants include, for example, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins, buffer substances, water, salts or electrolytes and cellulose-based substances. Preferred dosage forms include tablet, capsule, caplet, liquid, solution, suspension, emulsion, lozenges, syrup, reconstitutable powder, granule, suppository and transdermal patch. Dosage levels and requirements for the compounds of the present invention may be selected by those of ordinary skill in the art from available methods and techniques suitable for a particular patient. In some embodiments, dosage levels range from about 1-1000 mg/dose for a 70 kg patient. Although one dose per day may be sufficient, up to 5 doses per day may be given. For oral doses, up to 2000 mg/day may be required. As the skilled artisan will appreciate, lower or higher doses may be required depending on particular factors. For instance, specific dosage and treatment regimens will depend on factors such as the patient's general health profile, the severity and course of the patient's disorder or disposition thereto, and the judgment of the treating physician.

Claims

1. A method for treating a cardiovascular disease or condition in a warm-blooded animal which comprises administering to the animal a therapeutically effective amount of a compound of formula (I)

wherein:

R₁is chosen from

C_3-8cycloalkyl, C_3-8cycloalkylC_1-6alkyl, haloC_1-6alkyl, aminoC_1-6alkyl, hydroxyC_1-6alkyl, aryl, heteroaryl, heteroarylC_1-6alkyl, heterocyclyl, —C_1-3alkylOaryl, CH₂OCH₂aryl, —CH₂OC(O)C_1-6alkyl, —(CH₂)_1-3S(O)_0-2aryl, —(CH₂)_1-2S(O)_0-2C_1-6alkyl, —(CH₂)_1-3CO₂C_1-6alkyl, —(CH₂)_1-3NHC_1-6alkyl, —(CH₂)_1-3NHC_1-6alkylC_3-8cycloalkyl, —(CH₂)_1-2CN and —CH(R₃)N(R₄)(R₅)

wherein:

R₃is chosen from H, C_1-6alkyl, —(CH₂)_1-3aryl and —(CH₂)_1-3heteroaryl;

R₄is chosen from H, C_1-6alkyl, C_3-12cycloalkyl, C_3-7cycloalkylC_1-6alkyl, aryl, aryl(CH₂)_1-3, heteroaryl(CH₂)_1-3, C_1-3alkylO(CH₂)_1-3, tetrahydropyran-4-ylmethyl and (C_1-3alkyl)₂N(CH₂)_2-4—; and R₅is chosen from H and C_1-6alkyl;

or R₄and R₅together with the nitrogen atom they are connected to may form a heterocyclyl group;

wherein each aryl, arylalkyl, heteroaryl, heteroarylalkyl, cycloalkylalkyl, cycloalkyl and heterocyclyl group is optionally substituted with 1-3 groups selected from halogen, C_1-6alkoxy, C_3-8cycloalkyl, C_1-6alkyl, —CN, —NO₂, —OH, oxo, —CF₃, —OCF₃, —C_0-3alkylCO₂H, C_1-6alkylCO₂—, C_1-6alkylsulfonylC_0-3alkyl-, —SO₂C_1-6alkylNR₆R₇, —C_0-3alkylSO₂NR₆R₇, —C_0-3C(O)NR₆R₇, heteroaryl, heteroarylC_1-3alkyl, heterocyclyl, heterocyclylSO₂—, arylC_1-3alkyl, aryloxy, arylthio and C_0-3NR₆R₇;

wherein each aryl and heteroaryl group is optionally substituted with 1-3 groups selected from halogen, C_1-6alkoxy, C_1-6alkyl, —CN, —NO₂, —OH, —CF₃, —OCF₃, —C(O)NR₈R₉, —SO₂NR₈R₉, —SO₂Me and amino optionally substituted by one or two C_1-6alkyl groups or a C(O)C_1-6alkyl group;

R₂is chosen from H, halogen, C_1-6alkoxy, —CN, —CF₃and C_1-6alkyl;

R₆and R₇are independently selected from H, C_1-6alkyl, —C(O)C_1-6alkyl and —C_1-6alkylNH₂; or R₆and R₇, together with the nitrogen they are connected to may form a piperazine, piperidine or pyrrolidine ring;

R₈and R₉are independently selected from H and methyl;

R₁₀is selected from H, Cl and F;

X is CH; and

Y is chosen from —NHC(O)—;

or a tautomer or a salt of the compound of formula (I).

2. The method of claim 1, wherein the cardiovascular disease or condition is selected form the group consisting of hypertension, atherosclerosis, restenosis, stroke, heart failure, coronary vasospasm, cerebral vasospasm, ischemia/reperfusion injury, pulmonary hypertension, angina, myocardial infarction, peripheral artery disease, coronary artery disease and combinations thereof.

3. The method of claim 1, wherein:

R₁is chosen from C_3-8cycloalkyl, C_3-8cycloalkylC_1-6alkyl, phenyl, thienyl, pyridyl, isoxazolyl, pyrazolyl, thienylmethyl, piperidinyl, piperazinyl, pyrrolidinyl, tetrahydrofuranyl, tetrahydropyranyl, —CH₂Ophenyl, —CH₂OCH₂phenyl, —CH₂OC(O)C_1-6alkyl, —(CH₂)_1-3S(O)_0-2phenyl, —(CH₂)_1-2S(O)_0-2C_1-6alkyl, —(CH₂)_1-3CO₂C_1-6alkyl, —(CH₂)_1-3NHC_1-6alkyl, —(CH₂)_1-3NHC_1-6alkylC_3-8cycloalkyl, —(CH₂)_1-3CN and —CH(R₃)N(R₄)(R₅)

wherein:

R₃is chosen from H, C_1-6alkyl, benzyl, phenylethyl and pyridylmethyl;

R₄is chosen from H, C_1-6alkyl, C_3-12cycloalkyl, C_3-7cycloalkylC_1-6alkyl, phenyl, benzyl, thienylethyl, C_1-3alkylO(CH₂)_1-3, tetrahydropyran-4-ylmethyl and (C_1-3)₂N(CH₂)_2-4—;

and R₅is chosen from H and C_1-6alkyl;

or R₄and R₅together with the nitrogen atom they are connected to may form a piperidine, piperazine or thiomorpholine group;

wherein each cycloalkyl, cycloalkylalkyl, phenyl, benzyl, phenylethyl, thienyl, pyridyl, isoxazolyl, pyrazolyl, thienylmethyl, piperidinyl, piperazinyl, pyrrolidinyl, tetrahydrofuranyl, tetrahydropyranyl, and thiomorpholinyl group is optionally substituted with 1-3 groups selected from halogen, C_1-6alkoxy, C_1-6alkyl, —CN, —NO₂, —OH, —CF₃, —OCF₃, C_1-6alkylCO₂, C_1-6alkylsulfonyl, pyrimidyl, pyridyl, morpholinyl, benzyl, phenyloxy and phenylthio and amino optionally substituted by one or two C_1-6alkyl groups or a C(O)C_1-6alkyl group;

wherein each phenyl, benzyl, pyrimidinyl and pyridyl group is optionally substituted with 1-3 groups selected from halogen, C_1-6alkoxy, C_1-6alkyl, —CN, —NO₂, —OH, —CF₃, —OCF₃, —C(O)NR₈R₉, —SO₂NR₈R₉, —SO₂Me and amino optionally substituted by one or two C_1-6alkyl groups or a C(O)C_1-6alkyl group;

R₂is chosen from H, Br, Cl, —CN, —CF₃and methyl;

R₈and R₉are independently selected from H and methyl; and

R₁₀is selected from H, Cl and F;

or a tautomer or a salt of the compound of formula (I).

4. The method of claim 1, wherein:

R₁is chosen from cyclopentyl, cyclohexyl, phenyl, thienylmethyl, piperidinyl, pyrrolodinyl. —CH₂Sphenyl and —CH(R₃)N(R₄)(R₅)

wherein:

R₃is chosen from H, C_1-6alkyl, benzyl and phenylethyl;

R₄is chosen from H, C_1-6alkyl, C_3-12cycloalkyl, C_3-7cycloalkylmethyl, benzyl, thienylethyl, and tetrahydropyran-4-ylmethyl;

and R₅is chosen from H and methyl;

or R₄and R₅together with the nitrogen atom they are connected to may form a piperidine group;

wherein each cyclopentyl, cyclohexyl, phenyl, benzyl, phenylethyl, thienylmethyl, piperidinyl and pyrrolidinyl group is optionally substituted with 1-3 groups selected from halogen, C_1-6alkoxy, C_1-6alkyl, —CN, —NO₂, —OH, —CF₃, —OCF₃, and amino optionally substituted by one or two C_1-6alkyl groups or a C(O)C_1-6alkyl group;

R₂is chosen from H, Br and Cl; and

R₁₀is H;

or a tautomer or a salt of the compound of formula (I).

5. A method of treating renal disease, erectile dysfunction, asthma, glaucoma or organ failure resulting from hypertension in a warm-blooded animal which comprises administering to the animal a therapeutically effective amount of a compound of formula (I):

wherein:

R₁is chosen from

C_3-8cycloalkyl, C_3-8cycloalkylC_1-6alkyl, haloC_1-6alkyl, aminoC_1-6alkyl, hydroxyC_1-6alkyl, aryl, heteroaryl, heteroarylC_1-6alkyl, heterocyclyl, —C_1-3alkylOaryl, CH₂OCH₂aryl, —CH₂OC(O)C_1-6alkyl, —(CH₂)_1-3S(O)_0-2aryl, —(CH₂)_1-2S(O)_0-2C_1-6alkyl, —(CH₂)_1-3CO₂C_1-6alkyl, —(CH₂)_1-3NHC_1-6alkyl, —(CH₂)_1-3NHC_1-6alkylC_3-8cycloalkyl, —(CH₂)_1-2CN and —CH(R₃N(R₄)(R₅)

wherein:

R₄is chosen from H, C_1-6alkyl, C_3-12cycloalkyl, C_3-7cycloalkylC_1-6alkyl, aryl, aryl(CH₂)_1-3, heteroaryl(CH₂)_1-3, C_1-3alkylO(CH₂)_1-3, tetrahydropyran-4-ylmethyl and (C_1-3alkyl)₂N(CH₂)_2-4—;

and R₅is chosen from H and C_1-6alkyl;

wherein each aryl and heteroaryl group is optionally substituted with 1-3 groups selected from halogen, C_1-6alkoxy, C_1-6alkyl, —CN, —NO₂, OH, —CF₃, —OCF₃, —C(O)NR₈R₉, —SO₂NR₈R₉, —SO₂Me and amino optionally substituted by one or two C_1-6alkyl groups or a C(O)C_1-6alkyl group;

R₂is chosen from H, halogen, C_1-6alkoxy, —CN, —CF₃and C_1-6alkyl;

R₆and R₇are independently selected from H, C_1-6alkyl, —C(O)C_1-6alkyl and —C_1-6alkylNH₂; or R₆and

R₇together with the nitrogen they are connected to may form a piperazine, piperidine or pyrrolidine ring;

R₈and R₉are independently selected from H and methyl;

R₁₀is selected from H, Cl and F;

X is CH; and

Y is chosen from —NHC(O)—;

or a tautomer or a salt of the compound of formula (I).

6. The method of claim 5, wherein:

wherein:

R₃is chosen from H, C_1-6alkyl, benzyl, phenylethyl and pyridylmethyl;

R₄is chosen from H, C_1-6alkyl, C_3-12cycloalkyl, C_3-7cycloalkylC_1-6alkyl, phenyl, benzyl, thienylethyl, C_1-3alkylO(CH₂)_1-3, tetrahydropyran-4-ylmethyl and (C_1-3)₂N(CH₂)_2-4-;

and R₅is chosen from H and C_1-6alkyl;

wherein each cycloalkyl, cycloalkylalkyl, phenyl, benzyl, phenylethyl, thienyl, pyridyl, isoxazolyl, pyrazolyl, thienylmethyl, piperidinyl, piperazinyl, pyrrolidinyl, tetrahydrofuranyl, tetrahydropyranyl, and thiomorpholinyl group is optionally substituted with 1-3 groups selected from halogen, C_1-6alkoxy, C_1-6alkyl, —CN, —NO₂, —OH, —CF₃, —OCF₃, C_1-6alkylCO₂—, C_1-6alkylsulfonyl, pyrimidyl, pyridyl, morpholinyl, benzyl, phenyloxy and phenylthio and amino optionally substituted by one or two C_1-6alkyl groups or a C(O)C_1-6alkyl group;

R₂is chosen from H, Br, Cl, —CN, —CF₃and methyl;

R₈and R₉are independently selected from H and methyl; and

R₁₀is selected from H, Cl and F;

or a tautomer or a salt of the compound of formula (I).

7. The method of claim 5, wherein:

R₁is chosen from cyclopentyl, cyclohexyl, phenyl, thienylmethyl, piperidinyl, pyrrolodinyl, —CH₂Sphenyl and —CH(R₃)N(R₄)(R₅)

wherein:

R₃is chosen from H, C_1-6alkyl, benzyl and phenylethyl;

and R₅is chosen from H and methyl;

R₂is chosen from H, Br and Cl; and

R₁₀is H;

or a tautomer or a salt of the compound of formula (I).