US20120171684A1 - Ddao compounds as fluorescent reference standards - Google Patents

Ddao compounds as fluorescent reference standards Download PDF

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Publication number
US20120171684A1
US20120171684A1 US13/339,340 US201113339340A US2012171684A1 US 20120171684 A1 US20120171684 A1 US 20120171684A1 US 201113339340 A US201113339340 A US 201113339340A US 2012171684 A1 US2012171684 A1 US 2012171684A1
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United States
Prior art keywords
dye
reporter
substituted
dyes
alkyl
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Abandoned
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US13/339,340
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English (en)
Inventor
Scott C. Benson
Cinna Monighetti
Sandy M. Koepf
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Life Technologies Corp
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Life Technologies Corp
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Priority to US13/339,340 priority Critical patent/US20120171684A1/en
Assigned to Life Technologies Corporation reassignment Life Technologies Corporation ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: BENSON, SCOTT C., KOEPF, SANDY M., MONIGHETTI, Cinna
Publication of US20120171684A1 publication Critical patent/US20120171684A1/en
Priority to US14/071,552 priority patent/US9315859B2/en
Priority to US15/066,248 priority patent/US9790544B2/en
Priority to US15/707,157 priority patent/US10131936B2/en
Priority to US16/164,348 priority patent/US10988798B2/en
Priority to US17/219,551 priority patent/US20210292818A1/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B15/00—Acridine dyes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00—Reactions characterised by their quantitative nature
    • C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/101—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
US13/339,340 2010-12-29 2011-12-28 Ddao compounds as fluorescent reference standards Abandoned US20120171684A1 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
US13/339,340 US20120171684A1 (en) 2010-12-29 2011-12-28 Ddao compounds as fluorescent reference standards
US14/071,552 US9315859B2 (en) 2010-12-29 2013-11-04 DDAO compounds as fluorescent reference standards
US15/066,248 US9790544B2 (en) 2010-12-29 2016-03-10 DDAO compounds as fluorescent reference standards
US15/707,157 US10131936B2 (en) 2010-12-29 2017-09-18 DDAO compounds as fluorescent reference standards
US16/164,348 US10988798B2 (en) 2010-12-29 2018-10-18 DDAO compounds as fluorescent reference standards
US17/219,551 US20210292818A1 (en) 2010-12-29 2021-03-31 DDAO Compounds as Fluorescent Reference Standards

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201061428182P 2010-12-29 2010-12-29
US13/339,340 US20120171684A1 (en) 2010-12-29 2011-12-28 Ddao compounds as fluorescent reference standards

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US14/071,552 Continuation US9315859B2 (en) 2010-12-29 2013-11-04 DDAO compounds as fluorescent reference standards

Publications (1)

Publication Number Publication Date
US20120171684A1 true US20120171684A1 (en) 2012-07-05

Family

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Family Applications (6)

Application Number Title Priority Date Filing Date
US13/339,340 Abandoned US20120171684A1 (en) 2010-12-29 2011-12-28 Ddao compounds as fluorescent reference standards
US14/071,552 Active US9315859B2 (en) 2010-12-29 2013-11-04 DDAO compounds as fluorescent reference standards
US15/066,248 Active US9790544B2 (en) 2010-12-29 2016-03-10 DDAO compounds as fluorescent reference standards
US15/707,157 Active US10131936B2 (en) 2010-12-29 2017-09-18 DDAO compounds as fluorescent reference standards
US16/164,348 Active US10988798B2 (en) 2010-12-29 2018-10-18 DDAO compounds as fluorescent reference standards
US17/219,551 Abandoned US20210292818A1 (en) 2010-12-29 2021-03-31 DDAO Compounds as Fluorescent Reference Standards

Family Applications After (5)

Application Number Title Priority Date Filing Date
US14/071,552 Active US9315859B2 (en) 2010-12-29 2013-11-04 DDAO compounds as fluorescent reference standards
US15/066,248 Active US9790544B2 (en) 2010-12-29 2016-03-10 DDAO compounds as fluorescent reference standards
US15/707,157 Active US10131936B2 (en) 2010-12-29 2017-09-18 DDAO compounds as fluorescent reference standards
US16/164,348 Active US10988798B2 (en) 2010-12-29 2018-10-18 DDAO compounds as fluorescent reference standards
US17/219,551 Abandoned US20210292818A1 (en) 2010-12-29 2021-03-31 DDAO Compounds as Fluorescent Reference Standards

Country Status (4)

Country Link
US (6) US20120171684A1 (zh)
EP (2) EP2913408A1 (zh)
CN (2) CN103597093A (zh)
WO (1) WO2012092403A1 (zh)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160230213A1 (en) * 2013-10-14 2016-08-11 Agency For Science, Technology And Research Non-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution
JP2021529943A (ja) * 2018-06-28 2021-11-04 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 血液培養測定システムにおける信号を正規化するためのシステム及び方法

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2371927B1 (en) 2004-09-16 2020-03-11 Life Technologies Corporation Fluorescent dye compounds, conjugates and uses thereof
EP2913408A1 (en) 2010-12-29 2015-09-02 Life Technologies Corporation Ddao compounds as fluorescent reference standards
US10221444B1 (en) * 2017-11-05 2019-03-05 URIT Medical Electronic Co., Ltd. Methods and systems for production of DNA libraries directly from FFPE tissue
CN115803333A (zh) 2020-07-02 2023-03-14 生命技术公司 三核苷酸帽类似物、其制备和用途

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160230213A1 (en) * 2013-10-14 2016-08-11 Agency For Science, Technology And Research Non-motorized optical multiplexing for the simultaneous detection of dna target amplicons in a polymerase chain reaction solution
US10718012B2 (en) * 2013-10-14 2020-07-21 Agency For Science, Technology And Research Non-motorized optical multiplexing for the simultaneous detection of DNA target amplicons in a polymerase chain reaction solution
JP2021529943A (ja) * 2018-06-28 2021-11-04 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ 血液培養測定システムにおける信号を正規化するためのシステム及び方法

Also Published As

Publication number Publication date
US20140134615A1 (en) 2014-05-15
US20180127811A1 (en) 2018-05-10
US9315859B2 (en) 2016-04-19
EP2658998A1 (en) 2013-11-06
CN103597093A (zh) 2014-02-19
EP2913408A1 (en) 2015-09-02
US9790544B2 (en) 2017-10-17
US10988798B2 (en) 2021-04-27
US10131936B2 (en) 2018-11-20
CN107326073A (zh) 2017-11-07
US20160258004A1 (en) 2016-09-08
US20190119732A1 (en) 2019-04-25
WO2012092403A1 (en) 2012-07-05
US20210292818A1 (en) 2021-09-23
EP2658998B1 (en) 2015-04-22

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Legal Events

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AS Assignment

Owner name: LIFE TECHNOLOGIES CORPORATION, CALIFORNIA

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:BENSON, SCOTT C.;MONIGHETTI, CINNA;KOEPF, SANDY M.;SIGNING DATES FROM 20120103 TO 20120109;REEL/FRAME:027681/0883

STCB Information on status: application discontinuation

Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION