US20080175865A1 - Vaccine comprising lactobacilli for treating prostate inflammation and benign prostate hyperplasias - Google Patents

Vaccine comprising lactobacilli for treating prostate inflammation and benign prostate hyperplasias Download PDF

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Publication number
US20080175865A1
US20080175865A1 US12/077,780 US7778008A US2008175865A1 US 20080175865 A1 US20080175865 A1 US 20080175865A1 US 7778008 A US7778008 A US 7778008A US 2008175865 A1 US2008175865 A1 US 2008175865A1
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US
United States
Prior art keywords
lactobacillus
prostate
vaccine
lactobacillus strains
inflammation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US12/077,780
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English (en)
Inventor
Gyule VARGA
Ottilia UJHELYI
Tamas Ujhelyi
Erika LAZAR
Jozsef BARTUS
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Individual
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Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to US12/077,780 priority Critical patent/US20080175865A1/en
Publication of US20080175865A1 publication Critical patent/US20080175865A1/en
Priority to US13/049,344 priority patent/US9056073B2/en
Priority to US14/735,637 priority patent/US20160051657A1/en
Priority to US15/891,620 priority patent/US20180161414A1/en
Abandoned legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/02Bacterial antigens
    • A61K39/09Lactobacillales, e.g. aerococcus, enterococcus, lactobacillus, lactococcus, streptococcus
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66Microorganisms or materials therefrom
    • A61K35/74Bacteria
    • A61K35/741Probiotics
    • A61K35/744Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
    • A61K35/747Lactobacilli, e.g. L. acidophilus or L. brevis
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00Drugs for disorders of the urinary system
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00Drugs for disorders of the urinary system
    • A61P13/08Drugs for disorders of the urinary system of the prostate
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00Drugs for genital or sexual disorders; Contraceptives
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04Antibacterial agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P5/00Drugs for disorders of the endocrine system
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/51Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/521Bacterial cells; Fungal cells; Protozoal cells inactivated (killed)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K2039/55Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
    • A61K2039/552Veterinary vaccine
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66Microorganisms or materials therefrom
    • A61K35/74Bacteria
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00Medicinal preparations containing antigens or antibodies
    • A61K39/39Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants

Definitions

  • the present invention relates to a vaccine comprising Lactobacillus strains useful in treating prostate inflammation and benign prostate hyperplasias (stages I and II).
  • K. ⁇ jhelyi has found that necrosis can be induced also by Lactobacillus strains cultivated from vagina. Based on his observation, it can be stated that the body of the bacterium contains a toxin which is responsible for damaging the epithelia [ ⁇ jhelyi K. et al.: Role of Lactobacillus in urogenital inflammations and their treatment with vaccination, Symposium cum participations internationalis de Biocenosi Vaginae, Smolenie, 1983]. Certain strains, injected intradermally to the back of rabbits, cause necrosis of smaller or larger areas, while others cause necrosis only in higher concentration or do not cause necrosis at all. K.
  • rabbits can be immunized by vaccination against the necrotic effect.
  • He vaccinated rabbits intramuscularly with vaccine produced from certain Lactobacillus strains.
  • Trichomonas vaginalis contributes to the rise in vaginal pH by consuming lactic acid produced by Lactobacillus strains in the vagina, thereby promoting the over-proliferation of Lactobacillus strains. Consequently toxin is present in higher concentration which, by damaging the mucous membrane, causes cell necrosis.
  • Lactobacillus strains because of their receptor inhibiting and antibiotic activity as well as pH-modifying effect, are antagonistic to pyogenic microorganisms [Reddy et al.: Natural antibiotic activity of Lactobacillus, Dairy Prod. J 18:15-22 (1983); Salminen et al.: Lactic acid bacteria in the gut in normal and disordered states, Dig. Dis., 10:227-238 (1992)].
  • Lactobacillus strains can bind directly to T-lymphocytes since both the T-helper and T-killer cells have specific receptors for Lactobacillus strains. Furthermore, Lactobacillus strains promote the gamma-interferon production of the lymphocytes and the cytotoxic activity of the natural killer cells [De Simone C., et al.: Enhancement of immune response of murine Peyer's pothes by a diet supplemented with yoghurt, J. Immunopharmacol., 1:87-95 (1987)].
  • Lactobacillus strains aspecifically increase the production of IgM and IgG [Blocksma et al.: Adjuvant activity of lactobacilli, different effects of viable and killed bacteria, Clin. Exp. Immunol., 37:367-373]. Additionally, under experimental conditions, Lactobacillus strains show antitumour and macrophage-activating activity [Kato I. et al.,: Antitumor activity of Lactobacillus casei in mice, Gann, 72:517-523 (1983); Oda M. et al.: Antitumor polysaccharide from Lactobacillus sp., Agric Biol. Chem., 47:1623-1627 (1983)].
  • Lactobacillus strains used parenterally administered Lactobacillus strains for aspecific immunostimulation and observed that the Lactobacillus strains used, in contrast to other aspecific immunostimulation (e.g. by BCG, endotoxins etc.), show protective effect against certain bacterial toxins. This applies especially to toxic Lactobacillus strains.
  • the inventors of the present invention have, however, found that conditions in the prostate are favorable to the proliferation of Lactobacillus strains and that pathogenic lactobacilli can often be cultivated from patients suffering from chronic prostate inflammation and/or prostate hyperplasia. On this basis, therapeutic utilization of vaccines comprising Lactobacillus strains for treating such patients has been achieved.
  • the invention relates to vaccines for treating prostate inflammations and benign prostate hyperplasias (stages I and II) comprising Lactobacillus strains in inactivated form and carriers and/or excipients commonly used in vaccine preparations.
  • the invention relates to the use of Lactobacillus strains for producing vaccines capable of treating prostate inflammation and benign prostate hyperplasias (stages I and II).
  • the invention relates to the use of Lactobacillus strains for treating patients suffering from prostate inflammation and benign prostate hyperplasias (stages I and II).
  • the invention relates to a method of treating patients suffering from prostate inflammation and benign prostate hyperplasias (stages I and II) comprising administering an effective dose of a strain-suspension of Lactobacillus strains intramuscularly to a patient in need of such treatment.
  • the strain-suspension of Lactobacillus strains comprises a mixed population of the said strains in inactivated form.
  • the lactobacilli used in the vaccine of the invention are Lactobacillus strains used in the above-said vaccines Gynevac®, Gyantren® and Solco Trichovac® that previously have been cultivated from women suffering from gynecologic inflammations of bacterial origin.
  • the single cultivated strains can be use per se or in the form of a blend of the strains.
  • the vaccine of the invention can be produced by methods commonly used for preparing vaccines.
  • the cultivated strains are stored in lyophilized form, then, before use, they are propagated by culturing in Man-Rogosa-Sharpe medium at 45° C.
  • composition of the said medium and the preparation method are set forth below.
  • the obtained solution is adjusted to 3000 ml by the addition of sterile water, filtered on G4 filter, bottles in smaller volumes and sterilized at 121° C.
  • composition of the above-said salt solution is as follows: 28.75 g of MgSO 4 -7H 2 O, 6 g of MnSO 4 -2H 2 O and 1.7 of FeSO 4 -7H 2 O dissolved in 250 ml of sterile water.
  • the protein content of the vaccine (suspension) of the invention is at least 0.08 mg/ml, and may be up to 1 mg/ml or more, preferably from about 0.08 to about 0.32 mg/ml, more preferably about 0.16 mg/ml.
  • the dosage of the vaccine of the invention and the frequency of the administration depend on the conditions of the patient and the severity of the symptoms to be treated. The precise dose and frequency of administration should be specified by the practicing physician. During treatment, it is advantageous if the vaccine is administered intramuscularly in a volume of 1 ml, once a week for five weeks.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Endocrinology (AREA)
  • Reproductive Health (AREA)
  • Diabetes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
US12/077,780 1999-11-25 2008-03-21 Vaccine comprising lactobacilli for treating prostate inflammation and benign prostate hyperplasias Abandoned US20080175865A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US12/077,780 US20080175865A1 (en) 1999-11-25 2008-03-21 Vaccine comprising lactobacilli for treating prostate inflammation and benign prostate hyperplasias
US13/049,344 US9056073B2 (en) 1999-11-25 2011-03-16 Vaccine comprising lactobacilli for treating prostate inflammation and benign prostate hyperplasias
US14/735,637 US20160051657A1 (en) 1999-11-25 2015-06-10 Vaccine comprising lactobacilli for treating prostate inflammation and benign prostate hyperplasias
US15/891,620 US20180161414A1 (en) 1999-11-25 2018-02-08 Vaccine comprising lactobacillus strains for treating prostate inflammation and benign prostate hyperplasias

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
HUP9904408 1999-11-25
HU9904408A HU227086B1 (en) 1999-11-25 1999-11-25 Lactobacillus vaccine for treating prostata inflammatory and benign prostata hyperplasia
PCT/HU2000/000122 WO2001037862A2 (en) 1999-11-25 2000-11-23 A vaccine comprising lactobacilli for treating prostate inflammation and benign prostate hyperplasias
US13082302A 2002-09-16 2002-09-16
US12/077,780 US20080175865A1 (en) 1999-11-25 2008-03-21 Vaccine comprising lactobacilli for treating prostate inflammation and benign prostate hyperplasias

Related Parent Applications (3)

Application Number Title Priority Date Filing Date
PCT/HU2000/000122 Continuation WO2001037862A2 (en) 1999-11-25 2000-11-23 A vaccine comprising lactobacilli for treating prostate inflammation and benign prostate hyperplasias
US10130823 Continuation 2002-09-16
US13082302A Continuation 1999-11-25 2002-09-16

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/049,344 Continuation US9056073B2 (en) 1999-11-25 2011-03-16 Vaccine comprising lactobacilli for treating prostate inflammation and benign prostate hyperplasias

Publications (1)

Publication Number Publication Date
US20080175865A1 true US20080175865A1 (en) 2008-07-24

Family

ID=90014600

Family Applications (4)

Application Number Title Priority Date Filing Date
US12/077,780 Abandoned US20080175865A1 (en) 1999-11-25 2008-03-21 Vaccine comprising lactobacilli for treating prostate inflammation and benign prostate hyperplasias
US13/049,344 Expired - Fee Related US9056073B2 (en) 1999-11-25 2011-03-16 Vaccine comprising lactobacilli for treating prostate inflammation and benign prostate hyperplasias
US14/735,637 Abandoned US20160051657A1 (en) 1999-11-25 2015-06-10 Vaccine comprising lactobacilli for treating prostate inflammation and benign prostate hyperplasias
US15/891,620 Abandoned US20180161414A1 (en) 1999-11-25 2018-02-08 Vaccine comprising lactobacillus strains for treating prostate inflammation and benign prostate hyperplasias

Family Applications After (3)

Application Number Title Priority Date Filing Date
US13/049,344 Expired - Fee Related US9056073B2 (en) 1999-11-25 2011-03-16 Vaccine comprising lactobacilli for treating prostate inflammation and benign prostate hyperplasias
US14/735,637 Abandoned US20160051657A1 (en) 1999-11-25 2015-06-10 Vaccine comprising lactobacilli for treating prostate inflammation and benign prostate hyperplasias
US15/891,620 Abandoned US20180161414A1 (en) 1999-11-25 2018-02-08 Vaccine comprising lactobacillus strains for treating prostate inflammation and benign prostate hyperplasias

Country Status (20)

Country Link
US (4) US20080175865A1 (cs)
EP (1) EP1272216B1 (cs)
AT (1) ATE470452T1 (cs)
AU (1) AU779214B2 (cs)
CA (1) CA2394843C (cs)
CZ (1) CZ302662B6 (cs)
DE (1) DE60044539D1 (cs)
DK (1) DK1272216T3 (cs)
EA (1) EA005198B1 (cs)
ES (1) ES2348591T3 (cs)
HU (1) HU227086B1 (cs)
IL (2) IL149779A0 (cs)
PL (1) PL201804B1 (cs)
PT (1) PT1272216E (cs)
RO (1) RO122760B1 (cs)
RS (1) RS51201B (cs)
SI (1) SI20960B (cs)
SK (1) SK288132B6 (cs)
UA (1) UA78673C2 (cs)
WO (1) WO2001037862A2 (cs)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1723965A1 (en) 2005-05-18 2006-11-22 Stallergenes Sa Compositions for antigen-specific induction of immuno-tolerance via oral immunization
US9732131B2 (en) 2006-02-27 2017-08-15 Calviri, Inc. Identification and use of novopeptides for the treatment of cancer
US20110027348A1 (en) 2007-08-27 2011-02-03 Janos Feher Composition and method inhibiting inflammation
CA3065325A1 (en) 2017-06-02 2018-12-06 Arizona Board Of Regents On Behalf Of Arizona State University A method to create personalized cancer vaccines
US12025615B2 (en) 2017-09-15 2024-07-02 Arizona Board Of Regents On Behalf Of Arizona State University Methods of classifying response to immunotherapy for cancer
EP4038222A4 (en) 2019-10-02 2023-10-18 Arizona Board of Regents on behalf of Arizona State University METHODS AND COMPOSITIONS FOR IDENTIFYING NEOANTIGENS FOR USE IN THE TREATMENT AND PREVENTION OF CANCER

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH639280A5 (de) * 1977-12-23 1983-11-15 Solco Basel Ag Verfahren zur herstellung einer vakzine gegen das trichomonas-syndrom.
DE19522472C2 (de) * 1995-06-21 1998-07-02 Eugen Baumgaertner Herstellungsverfahren eines Fermentationsproduktes zur Behandlung von Prostataleiden
CN1150314C (zh) * 1997-08-21 2004-05-19 纽西兰乳品局 增强免疫的乳酸菌
IT1298918B1 (it) * 1998-02-20 2000-02-07 Mendes Srl Uso di batteri dotati di arginina deiminasi per indurre apoptosi e/o ridurre una reazione infiammatoria e composizioni farmaceutiche
IT1306716B1 (it) * 1999-06-21 2001-10-02 Mendes S U R L Associazione di batteri lattici e suo uso per la prevenzione e/o iltrattamento terapeutico di infezioni e di stati infiammatori.

Also Published As

Publication number Publication date
ES2348591T3 (es) 2010-12-09
PT1272216E (pt) 2010-09-14
AU1874101A (en) 2001-06-04
US20180161414A1 (en) 2018-06-14
HUP9904408A2 (hu) 2002-03-28
IL149779A (en) 2009-05-04
UA78673C2 (en) 2007-04-25
YU37402A (sh) 2005-06-10
CZ302662B6 (cs) 2011-08-24
PL201804B1 (pl) 2009-05-29
RS51201B (sr) 2010-12-31
AU779214B2 (en) 2005-01-13
EA005198B1 (ru) 2004-12-30
WO2001037862A3 (en) 2001-11-22
SI20960A (sl) 2003-02-28
CA2394843C (en) 2011-01-18
EP1272216B1 (en) 2010-06-09
US9056073B2 (en) 2015-06-16
PL355886A1 (en) 2004-05-31
HUP9904408D0 (en) 2000-01-28
US20110243990A1 (en) 2011-10-06
SI20960B (sl) 2011-01-31
SK7382002A3 (en) 2002-11-06
DE60044539D1 (de) 2010-07-22
IL149779A0 (en) 2002-11-10
CA2394843A1 (en) 2001-05-31
EP1272216A2 (en) 2003-01-08
RO122760B1 (ro) 2010-01-29
SK288132B6 (sk) 2013-10-02
DK1272216T3 (da) 2010-09-27
HU227086B1 (en) 2010-06-28
CZ20021771A3 (cs) 2002-11-13
ATE470452T1 (de) 2010-06-15
US20160051657A1 (en) 2016-02-25
WO2001037862A2 (en) 2001-05-31
EA200200602A1 (ru) 2002-10-31

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