US20080125462A1 - Cinnamate Salts Of A Beta-2 Adrenergic Agonist - Google Patents

Cinnamate Salts Of A Beta-2 Adrenergic Agonist Download PDF

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US20080125462A1
US20080125462A1 US11/722,801 US72280106A US2008125462A1 US 20080125462 A1 US20080125462 A1 US 20080125462A1 US 72280106 A US72280106 A US 72280106A US 2008125462 A1 US2008125462 A1 US 2008125462A1
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Prior art keywords
hydroxy
methyl
phenyl
cinnamate
salt
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US11/722,801
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Lois Elizabeth Vernon
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Glaxo Group Ltd
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Glaxo Group Ltd
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Priority claimed from GB0500513A external-priority patent/GB0500513D0/en
Priority claimed from GB0514199A external-priority patent/GB0514199D0/en
Priority claimed from GB0514200A external-priority patent/GB0514200D0/en
Application filed by Glaxo Group Ltd filed Critical Glaxo Group Ltd
Priority to US11/722,801 priority Critical patent/US20080125462A1/en
Assigned to GLAXO GROUP LIMITED reassignment GLAXO GROUP LIMITED ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: VERNON, LOIS ELIZABETH
Publication of US20080125462A1 publication Critical patent/US20080125462A1/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
    • C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
    • C07D215/16—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D215/20—Oxygen atoms
    • C07D215/24—Oxygen atoms attached in position 8
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
    • C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
    • C07D215/16—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D215/20—Oxygen atoms
    • C07D215/22—Oxygen atoms attached in position 2 or 4
    • C07D215/227—Oxygen atoms attached in position 2 or 4 only one oxygen atom which is attached in position 2
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/47—Quinolines; Isoquinolines
    • A61K31/4704—2-Quinolinones, e.g. carbostyril
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/06—Antiasthmatics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/08—Bronchodilators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/06—Antiabortive agents; Labour repressants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
    • C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
    • C07D215/16—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D215/20—Oxygen atoms
    • C07D215/24—Oxygen atoms attached in position 8
    • C07D215/26—Alcohols; Ethers thereof

Definitions

  • the present invention relates to novel salts of a ⁇ 2 adrenergic agonist.
  • the invention relates to novel crystalline salts of compound (A) defined below.
  • the invention provides a process for preparing said crystalline salts of compound (A) and to pharmaceutical compositions containing them and their use in medicine.
  • ⁇ 2 Adrenergic receptor agonists are recognized as effective drugs for the treatment of pulmonary diseases such as asthma and chronic obstructive pulmonary disease (including chronic bronchitis and emphysema). ⁇ 2 Adrenergic receptor agonists are also useful for treating premature labour, and are potentially useful for treating neurological disorders and cardiac disorders.
  • each of R 1 , R 2 , R 3 , and R 4 is independently selected from the group consisting of hydrogen, hydroxy, amino, halo, —CH 2 OH and —NHCHO, or R 1 and R 2 taken together are selected from the group consisting of —NHC( ⁇ O)CH ⁇ CH—, —CH ⁇ CHC( ⁇ O)NH—, —NHC( ⁇ O)S; and —SC( ⁇ O)NH—;
  • R 5 and R 6 is —[X—C 1-6 alkylenyl] n —NR 10 R 11 or C 1-6 alkylenyl-NR 12 R 13 , and the other of R 5 and R 6 is selected from the group consisting of hydrogen, hydroxy, C 1-4 alkoxy, and C 1-4 alkyl, wherein C 1-4 alkyl is optionally substituted with halo, wherein
  • each X is independently selected from the group consisting of —O—, —NH—, —S—, —NHSO 2 —, —SO 2 NH—, —NHC( ⁇ O)—, and —C( ⁇ O)NH—;
  • each of R 10 , R 11 , R 12 , and R 13 is independently hydrogen or C 1-4 alkyl; or
  • R 10 together with the nitrogen atom to which it is attached and a carbon atom of the adjacent C 1-6 alkylenyl, or R 12 and R 13 , together with the nitrogen atom to which they are attached, or R 12 , together with the nitrogen atom to which it is attached and a carbon atom of the adjacent C 1-6 alkylenyl, form a heterocyclic or heteroaryl ring having from 5 to 7 ring atoms, wherein the ring optionally contains an additional heteroatom selected from oxygen, nitrogen, and sulfur, wherein nitrogen is optionally substituted with —S(O) 2 —C 1-4 alkyl; and
  • n 1, 2, or 3;
  • each of R 7 , R 8 , and R 9 is independently hydrogen or C 1-6 alkyl
  • Example 1 of the aforementioned US applications is the compound:
  • Compound A 5-[(R)-2-(2- ⁇ 4-[4-(2-amino-2-methyl-propoxy)-phenylamino]-phenyl ⁇ -ethylamino)-1-hydroxy-ethyl]-8-hydroxy-1H-quinolin-2-one (hereinafter referred to as Compound A), which may be depicted by the structural formula (I):
  • the present invention provides a salt of 5-[(R)-2-(2- ⁇ 4-[4-(2-amino-2-methyl-propoxy)-phenylamino]-phenyl ⁇ -ethylamino)-1-hydroxy-ethyl]-8-hydroxy-1H-quinolin-2-one, selected from a cinnamate, 4-methoxycinnamate, di-(4-phenylcinnamate) and 4-methyl cinnamate salt.
  • the invention also provides a salt of the compound of formula (I) hereinabove, wherein said salt is selected from a cinnamate, 4-methoxycinnamate, di-(4-phenylcinnamate) and 4-methyl cinnamate salt.
  • the present invention provides a cinnamate salt of 5-[(R)-2-(2- ⁇ 4-[4-(2-amino-2-methyl-propoxy)-phenylamino]-phenyl ⁇ -ethylamino)-1-hydroxy-ethyl]-8-hydroxy-1H-quinolin-2-one.
  • the present invention provides a 4-methoxy cinnamate salt of 5-[(R)-2-(2- ⁇ 4-[4-(2-amino-2-methyl-propoxy)-phenylamino]-phenyl ⁇ -ethylamino)-1-hydroxy-ethyl]-8-hydroxy-1H-quinolin-2-one.
  • the present invention provides a di-(4-phenylcinnamate) salt of 5-[(R)-2-(2- ⁇ 4-[4-(2-amino-2-methyl-propoxy)-phenylamino]-phenyl ⁇ -ethylamino)-1-hydroxy-ethyl]-8-hydroxy-1H-quinolin-2-one.
  • the invention provides a 4-methyl cinnamate salt of 5-[(R)-2-(2- ⁇ 4-[4-(2-amino-2-methyl-propoxy)-phenylamino]-phenyl ⁇ -ethylamino)-1-hydroxy-ethyl]-8-hydroxy-1H-quinolin-2-one.
  • the invention provides a crystalline 4-methyl cinnamate salt of 5-[(R)-2-(2- ⁇ 4-[4-(2-amino-2-methyl-propoxy)-phenylamino]-phenyl ⁇ -ethylamino)-1-hydroxy-ethyl]-8-hydroxy-1H-quinolin-2-one.
  • the invention provides a crystalline cinnamate salt of 5-[(R)-2-(2- ⁇ 4-[4-(2-amino-2-methyl-propoxy)-phenylamino]-phenyl ⁇ -ethylamino)-1-hydroxy-ethyl]-8-hydroxy-1H-quinolin-2-one.
  • the invention provides a crystalline 4-methoxy cinnamate salt of 5-[(R)-2-(2- ⁇ 4-[4-(2-amino-2-methyl-propoxy)-phenylamino]-phenyl ⁇ -ethylamino)-1-hydroxy-ethyl]-8-hydroxy-1H-quinolin-2-one.
  • the invention provides a crystalline di-(4-phenylcinnamate) salt of 5-[(R)-2-(2- ⁇ 4-[4-(2-amino-2-methyl-propoxy)-phenylamino]-phenyl ⁇ -ethylamino)-1-hydroxy-ethyl]-8-hydroxy-1H-quinolin-2-one.
  • salts of the invention may have varying hydration states.
  • the present invention encompasses hydrates of salts of the invention.
  • the 4-methylcinnamate salt may be obtained as a dihydrate, or as a hydrate associated with 3.5 moles of water.
  • the present invention also encompasses solvates of salts of the invention.
  • Salts of the invention may also exist in different stoichiometries.
  • the salt with 4-phenylcinnamic acid is formed with 2 moles of acid to each mole of Compound A.
  • the compound of formula (I), which is depicted and named as the R-isomer with respect to the OH group, may be in admixture with the corresponding S-isomer and hence salts of the invention may also exist as mixtures of said isomers.
  • said admixture may contain at least 90%, for example at least 95% of the R-isomer.
  • the salts of the invention may be prepared by contacting a solution of compound A with an acid selected from cinnamic acid, 4-methoxy cinnamic acid, 4-phenylcinnamic acid or 4-methylcinnamic acid.
  • Compound A may for example be dissolved in an aqueous organic solution, for example in a mixture of tetrahydrofuran and water, or an aqueous alcohol such as aqueous industrial methylated spirit.
  • the reaction may be effected with stirring.
  • the reaction temperature may be in the range from 0° C. to 50° C., for example from 15° C. to 30° C. Crystallisation may occur spontaneously or it may be induced, for example by scratching or seeding.
  • a method for preparing a 4-methoxy cinnamate salt of Compound A which comprises contacting in solution Compound A and 4-methoxy cinnamic acid.
  • the present invention provides a method for preparing a cinnamate salt of compound A which comprises contacting in solution Compound A and cinnamic acid.
  • the present invention provides a method for preparing a di-4-phenyl cinnamate salt of compound A which comprises contacting in solution Compound A and 4-phenylcinnamic acid.
  • the present invention provides a method for preparing a 4-methyl cinnamate salt of compound A which comprises contacting in solution Compound A and 4-methyl cinnamic acid.
  • Compound A may itself be prepared using one of the general methods described in U.S. Patent Application No. 60/535,784, and US 2005/0159448A1 as described hereinafter. Compound A may also be prepared by the specific method described by the Reference Example hereinafter.
  • P 1 represents a hydroxy-protecting group
  • P 2 represents a hydroxy-protecting group
  • L represents a leaving group, such as bromo
  • a compound of formula 1 is first reacted with an aryl amine (2) to provide an intermediate of formula 3.
  • this reaction is conducted in an organic solvent in the presence of base and a transition metal catalyst and arylphosphine ligand with heating.
  • a useful catalyst for coupling of an aryl group to an aryl amine is tris(dibenzylideneacetone)dipalladium(0) together with rac-2,2′-bis(diphenylphosphino)-1,1′-binapthyl.
  • the reaction is typically heated at a temperature of between about 50° C. and about 120° C. for between about 0.25 and about 12 hours.
  • the protecting group P 1 is typically a silyl protecting group, which is typically removed from the intermediate of formula 3 using a fluoride or acid reagent, to provide an intermediate of formula 4.
  • the protecting group P 2 is typically a benzyl protecting group, which is typically removed from the intermediate of formula 4 by hydrogenation using a palladium on carbon catalyst, to provide the product.
  • the reaction of Scheme C is typically conducted in a polar aprotic solvent in the presence of base.
  • suitable solvents include dimethylsulfoxide, dimethyl formamide, dimethylacetamide and the like.
  • the reaction is typically heated at a temperature of between about 60° C. and about 140° C. for between about 0.25 and about 4 hours.
  • Intermediates 2 and 6 are available commercially or are prepared from readily available starting materials.
  • R 5 is —[O—C 1-6 alkylenyl] n —NR 10 R 11 and R 6 is hydrogen
  • intermediate 2′, of general formula 2 can be prepared by the process of Scheme D
  • R 5a is defined such that —OR 5a is —[O—C 1-6 alkylenyl] n —NR 10 R 11 .
  • the reaction is conducted in dimethylsulfoxide in the presence of sodium hydride.
  • the reaction of intermediate 9 with the compound HO—R 5a may be conducted in a solvent such as tetrahydrofuran, in the presence of a base such as potassium tert.-butoxide.
  • Intermediate 10 may be converted into Intermediate 2′ by hydrogenation, for example using palladium on charcoal, in a solvent such as industrial methylated spirits.
  • the present invention accordingly also provides a method for the prophylaxis or treatment of a clinical condition in a mammal, such as a human, for which a selective ⁇ 2 -adrenoreceptor agonist is indicated, which comprises administration of a therapeutically effective amount of a salt of the this invention.
  • the present invention provides such a method for the prophylaxis or treatment of a disease associated with reversible airways obstruction such as asthma, chronic obstructive pulmonary disease (COPD), respiratory tract infection or upper respiratory tract disease.
  • COPD chronic obstructive pulmonary disease
  • the present invention provides such a method for the prophylaxis or treatment of a clinical condition selected from premature labour, depression, congestive heart failure, skin diseases (e.g. inflammatory, allergic, psoriatic, and proliferative skin diseases), conditions where lowering peptic acidity is desirable (e.g. peptic and gastric ulceration) or muscle wasting disease.
  • a clinical condition selected from premature labour, depression, congestive heart failure, skin diseases (e.g. inflammatory, allergic, psoriatic, and proliferative skin diseases), conditions where lowering peptic acidity is desirable (e.g. peptic and gastric ulceration) or muscle wasting disease.
  • a salt of the invention for use in medical therapy, particularly, for use in the prophylaxis or treatment of a clinical condition in a mammal, such as a human, for which a selective ⁇ 2 -adrenoreceptor agonist is indicated.
  • a salt of the invention for the prophylaxis or treatment of a disease associated with reversible airways obstruction such as asthma, chronic obstructive pulmonary disease (COPD), respiratory tract infection or upper respiratory tract disease.
  • COPD chronic obstructive pulmonary disease
  • the present invention also provides the use of a salt of the invention in the manufacture of a medicament for the prophylaxis or treatment of a clinical condition for which a selective ⁇ 2 -adrenoreceptor agonist is indicated, for example a disease associated with reversible airways obstruction such as asthma, chronic obstructive pulmonary disease (COPD), respiratory tract infection or upper respiratory tract disease.
  • a salt of the invention in the manufacture of a medicament for the prophylaxis or treatment of a clinical condition selected from premature labour, depression, congestive heart failure, skin diseases (e.g. inflammatory, allergic, psoriatic, and proliferative skin diseases), conditions where lowering peptic acidity is desirable (e.g. peptic and gastric ulceration) and muscle wasting disease.
  • salts of the invention may be administered by inhalation at a dose of from 0.0005 mg to 10 mg, preferably from 0.005 mg to 0.5 mg, for example, from 0.05 mg to 0.5 mg.
  • the dose range for adult humans is generally from 0.0005 mg to 10 mg per day and preferably from 0.01 mg to 1 mg per day, most preferrably from 0.05 mg to 0.5 mg.
  • a salt of the present invention selected from Compound A cinnamate, Compound A 4-methoxycinnamate, Compound A di-(4-phenylcinnamate) and Compound A 4-methylcinnamate, to be administered alone, it is preferable to present it as a pharmaceutical formulation.
  • the present invention provides a pharmaceutical formulation comprising Compound A cinnamate, and a pharmaceutically acceptable carrier or excipient, and optionally one or more other therapeutic ingredients.
  • the present invention provides a pharmaceutical formulation comprising Compound A 4-methoxycinnamate, and a pharmaceutically acceptable carrier or excipient, and optionally one or more other therapeutic ingredients.
  • the present invention further provides a pharmaceutical formulation comprising Compound A di-(4-phenyl cinnamate), and a pharmaceutically acceptable carrier or excipient, and optionally one or more other therapeutic ingredients.
  • the present invention further provides a pharmaceutical formulation comprising Compound A 4-methyl cinnamate, and a pharmaceutically acceptable carrier or excipient, and optionally one or more other therapeutic ingredients.
  • the formulations include those suitable for oral, parenteral (including subcutaneous, intradermal, intramuscular, intravenous and intraarticular), inhalation (including fine particle dusts or mists which may be generated by means of various types of metered dose pressurised aerosols, nebulisers or insufflators), rectal and topical (including dermal, buccal, sublingual and intraocular) administration although the most suitable route may depend upon for example the condition and disorder of the recipient.
  • the formulations may conveniently be presented in unit dosage form and may be prepared by any of the methods well known in the art of pharmacy. All methods include the step of bringing a salt of the invention (hereinafter also referred to as ‘active ingredient’) into association with the carrier which constitutes one or more accessory ingredients. In general the formulations are prepared by uniformly and intimately bringing into association the active ingredient with liquid carriers or finely divided solid carriers or both and then, if necessary, shaping the product into the desired formulation.
  • Dry powder compositions for topical delivery to the lung by inhalation may, for example, be presented in capsules and cartridges of for example gelatine, or blisters of for example laminated aluminium foil, for use in an inhaler or insufflator.
  • Powder blend formulations generally contain a powder mix for inhalation of the active ingredient and a suitable powder base (carrier/diluent/excipient substance) such as mono-, di or poly-saccharides (eg. lactose or starch). Use of lactose is preferred.
  • Dry powder compositions may also include, in addition to the drug and carrier, a further excipient such as a sugar ester e.g. cellobiose octaacetate, calcium stearate or magnesium stearate.
  • Each capsule or cartridge may generally contain between 20 ⁇ g to 10 mg of a salt of the invention optionally in combination with another therapeutically active ingredient.
  • the compound of the invention may be presented without excipients.
  • Packaging of the formulation may be suitable for unit dose or multi-dose delivery.
  • the formulation can be pre-metered (eg as in Diskus, see GB 2242134, U.S. Pat. Nos. 6,632,666, 5,860,419, 5,873,360 and 5,590,645 or Diskhaler, see GB 2178965, 2129691 and 2169265, U.S. Pat. Nos.
  • the Diskus inhalation device comprises an elongate strip formed from a base sheet having a plurality of recesses spaced along its length and a lid sheet hermetically but peelably sealed thereto to define a plurality of containers, each container having therein an inhalable formulation containing a salt of the invention preferably combined with lactose.
  • the strip is sufficiently flexible to be wound into a roll.
  • the lid sheet and base sheet will preferably have leading end portions which are not sealed to one another and at least one of the said leading end portions is constructed to be attached to a winding means. Also, preferably the hermetic seal between the base and lid sheets extends over their whole width.
  • the lid sheet may preferably be peeled from the base sheet in a longitudinal direction from a first end of the said base sheet.
  • the formulation may be presented if desired together with one or more other therapeutic agents in an inhalation device wherein the individual therapeutic agents are administrable simultaneously but are stored separately (or wholly or partly stored separately for triple combinations), e.g. in separate pharmaceutical compositions, for example as described in WO 03/061743.
  • Spray compositions for topical delivery to the lung by inhalation may for example be formulated as aqueous solutions or suspensions or as aerosols delivered from pressurised packs, such as a metered dose inhaler, with the use of a suitable liquefied propellant.
  • Aerosol compositions suitable for inhalation can be either a suspension or a solution and generally contain a salt of the invention optionally in combination with another therapeutically active ingredient and a suitable propellant such as a fluorocarbon or hydrogen-containing chlorofluorocarbon or mixtures thereof, particularly hydrofluoroalkanes, e.g.
  • the aerosol composition may be excipient free or may optionally contain additional formulation excipients well known in the art such as surfactants e.g. oleic acid or lecithin and cosolvents e.g. ethanol. Pressurised formulations will generally be retained in a canister (e.g. an aluminium canister) closed with a valve (e.g. a metering valve) and fitted into an actuator provided with a mouthpiece.
  • a canister e.g. an aluminium canister
  • a valve e.g. a metering valve
  • Medicaments for administration by inhalation desirably have a controlled particle size.
  • the optimum particle size for inhalation into the bronchial system is usually 1-10 ⁇ m, preferably 2-5 ⁇ m. Particles having a size above 20 ⁇ m are generally too large when inhaled to reach the small airways.
  • the particles of the active ingredient as produced may be size reduced by conventional means e.g. by micronisation.
  • the desired fraction may be separated out by air classification or sieving.
  • the particles will be crystalline.
  • an excipient such as lactose is employed, generally, the particle size of the excipient will be much greater than the inhaled medicament within the present invention.
  • lactose When the excipient is lactose it will typically be present as milled lactose, wherein not more than 85% of lactose particles will have a MMD of 60-90 ⁇ m and not more than 15% will have a MMD of less than 15 ⁇ m.
  • Intranasal sprays may be formulated with aqueous or non-aqueous vehicles with the addition of agents such as thickening agents, buffer salts or acid or alkali to adjust the pH, isotonicity adjusting agents or anti-oxidants.
  • agents such as thickening agents, buffer salts or acid or alkali to adjust the pH, isotonicity adjusting agents or anti-oxidants.
  • Solutions for inhalation by nebulation may be formulated with an aqueous vehicle with the addition of agents such as acid or alkali, buffer salts, isotonicity adjusting agents or antimicrobials. They may be sterilised by filtration or heating in an autoclave, or presented as a non-sterile product.
  • Formulations of the present invention suitable for oral administration may be presented as discrete units such as capsules, cachets or tablets each containing a predetermined amount of the active ingredient; as a powder or granules; as a solution or a suspension in an aqueous liquid or a non-aqueous liquid; or as an oil-in-water liquid emulsion or a water-in-oil liquid emulsion.
  • the salts of the invention may also be presented as a bolus, electuary or paste.
  • a tablet may be made by compression or moulding, optionally with one or more accessory ingredients.
  • Compressed tablets may be prepared by compressing in a suitable machine the active ingredient in a free-flowing form such as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, lubricating, surface active or dispersing agent.
  • Moulded tablets may be made by moulding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent.
  • the tablets may optionally be coated or scored and may be formulated so as to provide slow or controlled release of the active ingredient therein.
  • Formulations for parenteral administration include aqueous and non-aqueous sterile injection solutions which may contain anti-oxidants, buffers, bacteriostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents.
  • the formulations may be presented in unit-dose or multi-dose containers, for example sealed ampoules and vials, and may be stored in a freeze-dried (lyophilised) condition requiring only the addition of the sterile liquid carrier, for example saline or water-for-injection, immediately prior to use.
  • Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and tablets of the kind previously described.
  • Formulations for rectal administration may be presented as a suppository with the usual carriers such as cocoa butter or polyethylene glycol.
  • Formulations for topical administration in the mouth include lozenges comprising the active ingredient in a flavoured basis such as sucrose and acacia or tragacanth, and pastilles comprising the active ingredient in a basis such as gelatin and glycerin or sucrose an acacia.
  • Preferred unit dosage formulations are those containing an effective dose, as hereinbefore recited, or an appropriate fraction thereof, of the active ingredient.
  • formulations of this invention may include other agents conventional in the art having regard to the type of formulation in question, for example those suitable for oral administration may include flavouring agents.
  • the compounds and pharmaceutical formulations according to the invention may be used in combination with or include one or more other therapeutic agents, for example selected from anti-inflammatory agents, anticholinergic agents (particularly an M 1 , M 2 , M 1 /M 2 or M 3 receptor antagonist), other ⁇ 2 -adrenoreceptor agonists, antiinfective agents (e.g. antibiotics, antivirals), or antihistamines.
  • anti-inflammatory agents for example selected from anti-inflammatory agents, anticholinergic agents (particularly an M 1 , M 2 , M 1 /M 2 or M 3 receptor antagonist), other ⁇ 2 -adrenoreceptor agonists, antiinfective agents (e.g. antibiotics, antivirals), or antihistamines.
  • the invention thus provides, in a further aspect, a combination comprising a salt of the invention together with one or more other therapeutically active agents, for example selected from an anti-inflammatory agent (for example a corticosteroid or an NSAID), an anticholinergic agent, another ⁇ 2 -adrenoreceptor agonist, an antiinfective agent (e.g. an antibiotic or an antiviral), or an antihistamine.
  • an anti-inflammatory agent for example a corticosteroid or an NSAID
  • an anticholinergic agent for example a corticosteroid or an NSAID
  • another ⁇ 2 -adrenoreceptor agonist e.g. an antibiotic or an antiviral
  • an antiinfective agent e.g. an antibiotic or an antiviral
  • antihistamine e.g. an antibiotic or an antiviral
  • combinations are those comprising a salt of the invention together with a corticosteroid, and/or an anticholinergic, and/or a
  • the other therapeutic ingredient(s) may be used in the form of salts, (e.g. as alkali metal or amine salts or as acid addition salts), or prodrugs, or as esters (e.g. lower alkyl esters), or as solvates (e.g. hydrates) to optimise the activity and/or stability and/or physical characteristics (e.g. solubility) of the therapeutic ingredient.
  • the therapeutic ingredients may be used in optically pure form.
  • anti-inflammatory agents examples include corticosteroids and NSAIDs.
  • Corticosteroids which may be used in combination with the compounds of the invention are those oral and inhaled corticosteroids and their pro-drugs which have anti-inflammatory activity. Examples include methyl prednisolone, prednisolone, dexamethasone, fluticasone propionate, 6 ⁇ ,9 ⁇ -difluoro-17 ⁇ -[(2-furanylcarbonyl)oxy]-11 ⁇ -hydroxy-16 ⁇ -methyl-3-oxo-androsta-1,4-diene-17 ⁇ -carbothioic acid S-fluoromethyl ester, 6 ⁇ ,9 ⁇ -difluoro-11 ⁇ -hydroxy-16 ⁇ -methyl-3-oxo-17 ⁇ -propionyloxy-androsta-1,4-diene-17 ⁇ -carbothioic acid S-(2-oxo-tetrahydro-furan-3S-yl) ester, beclomethasone esters (e.g.
  • the 17-propionate ester or the 17,21-dipropionate ester the 17-propionate ester or the 17,21-dipropionate ester
  • budesonide flunisolide
  • mometasone esters e.g. the furoate ester
  • triamcinolone acetonide e.g. the furoate ester
  • rofleponide triamcinolone acetonide
  • ciclesonide butixocort propionate
  • RPR-106541 the 17-propionate ester or the 17,21-dipropionate ester
  • ST-126 the 17-propionate ester or the 17,21-dipropionate ester
  • Preferred corticosteroids include fluticasone propionate, 6 ⁇ ,9 ⁇ -difluoro-11 ⁇ -hydroxy-16 ⁇ -methyl-17 ⁇ -[(4-methyl-1,3-thiazole-5-carbonyl)oxy]-3-oxo-androsta-1,4-diene-17 ⁇ -carbothioic acid S-fluoromethyl ester and 6 ⁇ ,9 ⁇ -difluoro-17 ⁇ -[(2-furanylcarbonyl)oxy]-11 ⁇ -hydroxy-16 ⁇ -methyl-3-oxo-androsta-1,4-diene-17 ⁇ -carbothioic acid S-fluoromethyl ester, more preferably 6 ⁇ ,9 ⁇ -difluoro-17 ⁇ -[(2-furanylcarbonyl)oxy]-11 ⁇ -hydroxy-16 ⁇ -methyl-3-oxo-androsta-1,4-diene-17 ⁇ -carbothioic acid S-fluoromethyl ester.
  • NSAIDs examples include sodium cromoglicate, nedocromil sodium, phosphodiesterase (PDE) inhibitors (e.g. theophylline, PDE4 inhibitors or mixed PDE3/PDE4 inhibitors), leukotriene antagonists, inhibitors of leukotriene synthesis, iNOS inhibitors, tryptase and elastase inhibitors, beta-2 integrin antagonists and adenosine receptor agonists or antagonists (e.g. adenosine 2a agonists), cytokine antagonists (e.g. chemokine antagonists) or inhibitors of cytokine synthesis.
  • PDE phosphodiesterase
  • leukotriene antagonists inhibitors of leukotriene synthesis
  • iNOS inhibitors tryptase and elastase inhibitors
  • beta-2 integrin antagonists and adenosine receptor agonists or antagonists e.g. adenosine 2a
  • ⁇ 2 -adrenoreceptor agonists include salmeterol (e.g. as the xinofoate), salbutamol (e.g. as the sulphate or the free base), formoterol (e.g. as the fumarate), fenoterol or terbutaline and salts thereof.
  • a salt of the invention may be used in combination with a phosphodiesterase 4 (PDE4) inhibitor or a mixed PDE3/PDE4 inhibitor.
  • PDE4-specific inhibitor useful in this aspect of the invention may be any compound that is known to inhibit the PDE4 enzyme or which is discovered to act as a PDE4 inhibitor, and which are only PDE4 inhibitors, not compounds which inhibit other members of the PDE family as well as PDE4.
  • PDE4 inhibitor which has an IC 50 ratio of about 0.1 or greater as regards the IC 50 for the PDE4 catalytic form which binds rolipram with a high affinity divided by the IC 50 for the form which binds rolipram with a low affinity.
  • the cAMP catalytic site which binds R and S rolipram with a low affinity is denominated the “low affinity” binding site (LPDE 4) and the other form of this catalytic site which binds rolipram with a high affinity is denominated the “high affinity” binding site (HPDE 4).
  • LPDE4 low affinity binding site
  • HPDE 4 high affinity binding site
  • Particular PDE4 inhibitors are those which have an IC 50 ratio of greater than 0.5, and particularly those compounds having a ratio of greater than 1.0.
  • Examples of compounds include cis 4-cyano-4-(3-cyclopentyloxy-4-methoxyphenyl)cyclohexan-1-carboxylic acid, 2-carbomethoxy-4-cyano-4-(3-cyclopropylmethoxy-4-difluoromethoxyphenyl)cyclohexan-1-one and cis-[4-cyano-4-(3-cyclopropylmethoxy-4-difluoromethoxyphenyl)cyclohexan-1-ol]; these are examples of compounds which bind preferentially to the low affinity binding site and which have an IC 50 ratio of 0.1 or greater.
  • PDE-4 and mixed PDE3/PDE4 inhibitors include those listed in WO01/13953, the disclosure of which is hereby incorporated by reference.
  • anticholinergic agents are those compounds that act as antagonists at the muscarinic receptor, in particular those compounds which are antagonists of the M 1 and M 2 receptors.
  • exemplary compounds include the alkaloids of the belladonna plants as illustrated by the likes of atropine, scopolamine, homatropine, hyoscyamine; these compounds are normally administered as a salt, being tertiary amines.
  • These drugs, particularly the salt forms are readily available from a number of commercial sources or can be made or prepared from literature data via, to wit:
  • Atropine CAS-51-55-8 or CAS-51-48-1 (anhydrous form), atropine sulfate—CAS-5908-99-6; atropine oxide—CAS-4438-22-6 or its HCl salt—CAS-4574-60-1 and methylatropine nitrate—CAS-52-88-0.
  • Scopolamine CAS-51-34-3, hydrobromide salt—CAS-6533-68-2, methylbromide salt—CAS-155-41-9.
  • Particular anticholinergics include ipratropium (e.g. as the bromide), sold under the name Atrovent, oxitropium (e.g. as the bromide) and tiotropium (e.g. as the bromide) (CAS-139404-48-1). Also of interest are: methantheline (CAS-53-46-3), propantheline bromide (CAS-50-34-9), anisotropine methyl bromide or Valpin 50 (CAS-80-50-2), clidinium bromide (Quarzan, CAS-3485-62-9), copyrrolate (Robinul), isopropamide iodide (CAS-71-81-8), mepenzolate bromide (U.S. Pat. No.
  • antihistamines also referred to as H 1 -receptor antagonists
  • H 1 -receptor antagonists include any one or more of the numerous antagonists known which inhibit H 1 -receptors, and are safe for human use. All are reversible, competitive inhibitors of the interaction of histamine with H 1 -receptors. The majority of these inhibitors, mostly first generation antagonists, have a core structure, which can be represented by the following formula:
  • This generalized structure represents three types of antihistamines generally available: ethanolamines, ethylenediamines, and alkylamines.
  • first generation antihistamines include those which can be characterized as based on piperizine and phenothiazines.
  • Second generation antagonists which are non-sedating, have a similar structure-activity relationship in that they retain the core ethylene group (the alkylamines) or mimic the tertiary amine group with piperizine or piperidine.
  • Exemplary antagonists are as follows:
  • Ethanolamines carbinoxamine maleate, clemastine fumarate, diphenylhydramine hydrochloride, and dimenhydrinate.
  • Ethylenediamines pyrilamine amleate, tripelennamine HCl, and tripelennamine citrate.
  • Alkylamines chloropheniramine and its salts such as the maleate salt, and acrivastine.
  • Piperazines hydroxyzine HCl, hydroxyzine pamoate, cyclizine HCl, cyclizine lactate, meclizine HCl, and cetirizine HCl.
  • Piperidines Astemizole, levocabastine HCl, loratadine or its descarboethoxy analogue, and terfenadine and fexofenadine hydrochloride or another pharmaceutically acceptable salt.
  • Azelastine hydrochloride is yet another H 1 receptor antagonist which may be used in combination with a PDE4 inhibitor.
  • Examples of preferred anti-histamines include methapyrilene and loratadine.
  • the invention thus provides, in a further aspect, a combination comprising a salt of the invention together with a PDE4 inhibitor.
  • the invention thus provides, in a further aspect, a combination comprising a salt of the invention together with a corticosteroid, e.g. fluticasone propionate, 6 ⁇ ,9 ⁇ -difluoro-11 ⁇ -hydroxy-16 ⁇ -methyl-17 ⁇ -[(4-methyl-1,3-thiazole-5-carbonyl)oxy]-3-oxo-androsta-1,4-diene-17 ⁇ -carbothioic acid S-fluoromethyl ester or 6 ⁇ ,9 ⁇ -difluoro-17 ⁇ -[(2-furanylcarbonyl)oxy]-11 ⁇ -hydroxy-16 ⁇ -methyl-3-oxo-androsta-1,4-diene-17 ⁇ -carbothioic acid S-fluoromethyl ester.
  • a corticosteroid e.g. fluticasone propionate, 6 ⁇ ,9 ⁇ -difluoro-11 ⁇ -hydroxy-16 ⁇ -methyl-17 ⁇ -[(4-methyl-1,3-thiazole-5-carbonyl)oxy]-3-o
  • the invention provides, in a further aspect, a combination comprising a salt of the invention together with an antihistamine.
  • the invention provides, in a further aspect, a combination comprising a salt of the invention together with a PDE4 inhibitor and a corticosteroid, e.g. with an antihistamine and a corticosteroid as described hereinabove.
  • the invention provides, in a further aspect, a combination comprising a salt of the invention together with an anticholinergic and a PDE-4 inhibitor, e.g. with a PDE4 inhibitor and an anticholinergic as described hereinabove.
  • compositions comprising a combination as defined above together with a physiologically acceptable diluent or carrier represent a further aspect of the invention.
  • the individual compounds of such combinations may be administered either sequentially or simultaneously in separate or combined pharmaceutical formulations. Appropriate doses of known therapeutic agents will be readily appreciated by those skilled in the art.
  • FIG. 1 shows an x-ray powder diffraction pattern of 5-[(R)-2-(2- ⁇ 4-[4-(2-amino-2-methyl-propoxy)-phenylamino]-phenyl ⁇ -ethylamino)-1-hydroxy-ethyl]-8-hydroxy-1H-quinolin-2-one cinnamate.
  • FIG. 2 shows an x-ray powder diffraction pattern of 5-[(R)-2-(2- ⁇ 4-[4-(2-amino-2-methyl-propoxy)-phenylamino]-phenyl ⁇ -ethylamino)-1-hydroxy-ethyl]-8-hydroxy-1H-quinolin-2-one 4-methoxycinnamate.
  • FIG. 3 shows an x-ray powder diffraction pattern of 5-[(R)-2-(2- ⁇ 4-[4-(2-amino-2-methyl-propoxy)-phenylamino]-phenyl ⁇ -ethylamino)-1-hydroxy-ethyl]-8-hydroxy-1H-quinolin-2-one di-(4-phenylcinnamate).
  • FIG. 4 shows an x-ray powder diffraction pattern of 5-[(R)-2-(2- ⁇ 4-[4-(2-amino-2-methyl-propoxy)-phenylamino]-phenyl ⁇ -ethylamino)-1-hydroxy-ethyl]-8-hydroxy-1H-quinolin-2-one 4-methylcinnamate (prepared according to the method of Example 4, Method 1)
  • FIG. 5 shows an x-ray powder diffraction pattern of 5-[(R)-2-(2- ⁇ 4-[4-(2-amino-2-methyl-propoxy)-phenylamino]-phenyl ⁇ -ethylamino)-1-hydroxy-ethyl]-8-hydroxy-1H-quinolin-2-one 4-methylcinnamate (prepared according to the method of Example 4, Method 2).
  • XRPD analysis was performed on a Panalytical X-ray powder diffractometer, model X'Pert Pro PW3040/60, serial number DY1850. The method runs from 2 to 40 degrees 2-Theta with a 0.0167 degree 2-Theta step size and a 31.75 second collection time at each step using an X'celerator detector.
  • Example 1 the differential scanning calorimetry analysis was obtained using a Perkin Elmer Pyris 1, serial number 537N9062304. Samples were weighed into an aluminium pan, an aluminium lid placed on top of the sample and compressed with a brass rod. An empty pan and lid served as reference. Samples were equilibrated at 30° C. and heated at 10° C./min to 300° C. The instrument was calibrated using indium, tin and lead standards.
  • thermogravimetric analysis was obtained using a TA Instruments 2950 TGA, serial number HA2950-226. Samples were placed into a tared aluminium pan and then positioned on a platinum crucible. Samples were heated from ambient at 10° C./min to a temperature between 250 and 350° C. The instrument was calibrated for temperature using the Curie point of nickel and alumel.
  • reagents starting material and solvents were purchased from commercial suppliers, for example Sigma-Aldrich (St. Louis, Mo.), J. T. Baker (Phillipsburg, N.J.), and Honeywell Burdick and Jackson (Muskegon, Mi), and used without further purification; reactions were run under nitrogen atmosphere; reaction mixtures were monitored by thin layer chromatography (silica TLC), analytical high performance liquid chromatography (anal.
  • the reaction was quenched with water (1000 mL), extracted with dichloromethane (500 mL), and the organic layer washed (1:1 saturated aqueous sodium chloride:water, 1000 mL).
  • the product was precipitated by addition of 3M hydrochloric acid (400 mL) to the organic layer. The resulting orange solid was then filtered and washed with dichloromethane until the filtrate was colourless.
  • the solid material was immediately transferred to a hydrogenation flask.
  • step a A mixture of the product of step a (23.2 g, 1.1 equiv), 8-benzyloxy-5- ⁇ (R)-2-[2-(4-bromo-phenyl)-ethylamino-1-(tert-butyl-dimethyl-silanyloxy)-ethyl ⁇ -1H-quinolin-2-one hydrochloride (66.0 g, 0.1 mol), and sodium tert-butoxide (54.0 g, 5.5 equiv) in toluene (600 mL) was stirred at 90° C. until a homogenous solution was obtained.
  • the product of the previous step was treated with triethylamine trihydrofluoride (36 g) in 2-propanol (500 mL)/ethanol (100 mL) at room temperature for 16 h.
  • the mixture was concentrated under reduced pressure to one third of its original volume.
  • 1M aqueous sodium hydroxide (500 mL) was added, followed by acetonitrile (500 mL) and isopropyl acetate (500 mL).
  • the aqueous layer was removed and the organic phase washed with 1:1 saturated aqueous sodium chloride:water (400 mL) then saturated aqueous sodium chloride (400 mL).
  • the organics were dried over sodium sulfate and the solvent removed in vacuo to afford the title intermediate (50 g crude) as a brown solid, which was used without further purification.
  • DSC The sample exhibits an endotherm with an onset of around 60° C. This is followed by a second endotherm with an onset of 106° C. and a subsequent decomposition.
  • TGA The sample exhibits a weight loss of 2.8% w/w from ambient to approximately 63° C. This is followed by a second weight loss of 5.4% w/w from 63° C. to approximately 108° C. Subsequent weight loss is due to decomposition.
  • DSC The sample shows an endothermic event with an onset of around 112° C. followed by decomposition.
  • TGA The sample exhibits a weight loss of 5.7% w/w from ambient to approximately 125° C. This is followed by a second weight loss of 2.3% w/w from 125° C. to approximately 170° C. due to decomposition.
  • the filtered solid was washed with aqueous tetrahydrofuran (1:1 THF:H 2 O, 2 ⁇ 0.2 mL). Having been left to sit at ambient conditions for several hours, the wet solid was dried overnight at 60° C. in vacuo to give the title compound.
  • DSC The sample shows an endothermic event with an onset of around 60° C. This is followed by a second endotherm with an onset of 145° C. and subsequent decomposition.
  • DSC The sample shows an endothermic event with an onset of around 106° C. This is followed by decomposition.
  • TGA The sample exhibits a weight loss of 6.1% w/w from ambient to approximately 120° C. This is followed by a second weight loss of 2.0% w/w and decomposition.
  • the XRPD pattern of this product is shown in FIG. 4 .
  • DSC The sample shows an endothermic event with an onset of around 67° C. This is followed by second endotherm with an onset of around 113° C. and then decomposition.
  • TGA The sample exhibits a weight loss of 3.7% w/w from ambient to approximately 75° C. This is followed by a second weight loss of 5.1% w/w from 75 to approximately 125° C., a third weight loss of 2.1% w/w and decomposition.
  • the XRPD pattern of this product is shown in FIG. 5 .
  • 4-methyl cinnamate 3.5 hydrate (134.9 g), prepared by the above method, was further dried in a vacuum oven at 40° C. with a vacuum pump to reduce to significantly below 100 mbar. Most of the resulting solid (67.7 g) was slurried in 5% vol/vol aqueous IMS (500 mL, 7.4 vols) for 7 hours. The slurry was filtered and the cake washed with 5% vol/vol aqueous IMS (70 mL ⁇ 2, 2 ⁇ 1 vol). The cake was dried in a vacuum oven (42° C., ⁇ 100 mbar) overnight to afford the title compound (61.8 g).
  • XRPD of the product matches that shown previously in FIG. 4 .
  • 1,4-Dioxane (4.33 L) was added to a mixture of ⁇ 4-[(2-amino-2-methylpropyl)oxy]phenyl ⁇ amine dihydrochloride (406.7 g, 1.61 mol), 8-benzyloxy-5- ⁇ (R)-2-[2-(4-bromo-phenyl)-ethylamino-1-(tert-butyl-dimethyl-silanyloxy)-ethyl ⁇ -1H-quinolin-2-one hydrochloride (865.4 g, 1.34 mol), sodium tert-butoxide (900 g, 9.36 mol), bis(dibenzylideneacetone) palladium (9.4 g) and BINAP (rac-2,2′-bis(diphenylphosphino)-1,1′-binapthyl, 15.58 g).
  • the mixture was stirred and heated at 87 ⁇ 3° C. for 4 hours.
  • the mixture was cooled to room temperature, quenched with water (2.60 L) and passed through a CUNO zetacarbon filter.
  • Methyl acetate (2.60 L) and saturated sodium chloride solution made with 1.66 L water) were added, the solution mixed and the phases settled and separated.
  • the organic phase was washed with sodium chloride solution (623 g in 2.84 L water).
  • Methanol (3.46 L) and concentrated hydrochloric acid (735.6 mL) were added to the reaction mixture which was heated to 50 ⁇ 3° C. for 17 hours before returning to 20 ⁇ 3° C.
  • the foam was redissolved in methanol (1.2 L). A portion of the solution (1069 g) that contained 500 g of the crude material was injected onto a column in a biotage flash 150 system and eluted isocratically (99 parts methanol, 1 part 2M ammonia in methanol).
  • the catalyst was filtered off on a bed of celite which wastwice with IMS (300 mL then 150 mL) and then with aqueous IMS (2:1 IMS:water, 150 mL). This aqueous alcoholic free base solution could then be used directly for salt formation or dried and evaporated to give a solid which could be re-dissolved for salt formation.

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CA2660707C (fr) 2006-08-10 2014-07-08 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Preparation de (r,r)-fenoterol et d'analogues de (r,r)- ou (r,s)-fenoterol et leur utilisation pour le traitement d'une insuffisance cardiaque congestive
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AU2011224241B2 (en) 2010-03-10 2014-05-08 Sri International The use of fenoterol and fenoterol analogues in the treatment of glioblastomas and astrocytomas
UY33597A (es) 2010-09-09 2012-04-30 Irm Llc Compuestos y composiciones como inhibidores de la trk
WO2012034095A1 (fr) 2010-09-09 2012-03-15 Irm Llc Composés et compositions comme inhibiteurs de trk
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US20060189654A1 (en) * 2005-01-11 2006-08-24 Vernon Lois E Novel compounds

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060189654A1 (en) * 2005-01-11 2006-08-24 Vernon Lois E Novel compounds
US7772250B2 (en) * 2005-01-11 2010-08-10 Theravance, Inc. Compounds

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JP2008526805A (ja) 2008-07-24
EA200701260A1 (ru) 2008-02-28
WO2006074897A1 (fr) 2006-07-20
CN101137625A (zh) 2008-03-05
KR20070097106A (ko) 2007-10-02
US7772250B2 (en) 2010-08-10
MA29167B1 (fr) 2008-01-02
EP1846374A1 (fr) 2007-10-24
AU2006205933A1 (en) 2006-07-20
IL184246A0 (en) 2007-10-31
BRPI0606712A2 (pt) 2010-03-16
US20060189654A1 (en) 2006-08-24
MX2007008459A (es) 2007-07-25
NZ556335A (en) 2009-07-31
NO20073282L (no) 2007-09-04

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