US20030153074A1 - Method for extending the useful shelf-life of refrigerated red blood cells by nutrient supplementation - Google Patents

Method for extending the useful shelf-life of refrigerated red blood cells by nutrient supplementation Download PDF

Info

Publication number
US20030153074A1
US20030153074A1 US10/295,772 US29577202A US2003153074A1 US 20030153074 A1 US20030153074 A1 US 20030153074A1 US 29577202 A US29577202 A US 29577202A US 2003153074 A1 US2003153074 A1 US 2003153074A1
Authority
US
United States
Prior art keywords
red blood
blood cells
oxygen
metabolic
refrigerated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US10/295,772
Other languages
English (en)
Inventor
Mark Bitensky
Tatsuro Yoshida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boston University
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to US10/295,772 priority Critical patent/US20030153074A1/en
Assigned to HEMANEXT, LLC reassignment HEMANEXT, LLC ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: BITENSKY, MARK W., YOSHIDA, TATSURO
Publication of US20030153074A1 publication Critical patent/US20030153074A1/en
Priority to US11/138,135 priority patent/US7723017B2/en
Assigned to THE TRUSTEES OF BOSTON UNIVERSITY reassignment THE TRUSTEES OF BOSTON UNIVERSITY ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: HEMANEXT HOLDINGS, LLC
Assigned to NAVY, SECRETARY OF THE UNITED STATES OF AMERICA reassignment NAVY, SECRETARY OF THE UNITED STATES OF AMERICA CONFIRMATORY LICENSE (SEE DOCUMENT FOR DETAILS). Assignors: BOSTON UNIVERSITY
Abandoned legal-status Critical Current

Links

Images

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0205Chemical aspects
    • A01N1/021Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0226Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Definitions

  • the present invention relates to the preservation of blood in liquid form. More particularly, the present invention relates to enhancement of the shelf-life of oxygen-depleted refrigerated storage of red blood cells. Still more particularly, compositions and methodology involving nutrient or metabolic supplementation of red blood cells stored in liquid form in oxygen-depleted refrigeration are provided.
  • This invention was made with partial support by the United States Office of Naval Research, Contract No. N00014-98-1-0451. The Government has certain rights in the invention.
  • Red blood cells may, for example, be stored under refrigeration at a temperature above freezing (4° C.) as packed blood cell preparations. Red blood cells may be concentrated from whole blood with separation of the liquid blood component (plasma). Expired blood cannot be used and is discarded. There are periodic shortages of blood that occur due to donation fluctuation, emergencies and other factors. The logistics of blood supply and distribution impact the military, especially during times of combat, and remote hospitals or medical facilities. There is currently a need for the storage of autologous blood to avoid the significant risks of infection associated with non-autologous donor blood. Patients currently cannot collect and store with current technology enough autologous blood for certain pre-planned surgeries, including hip replacement, organ transplantation and the like.
  • frozen blood has limitations. For a number of years, frozen blood has been used by blood banks and the military for certain high-demand and rare types of blood. However, frozen blood is difficult to handle. It must be thawed which makes it impractical for emergency situations. Once blood is thawed, it must be used within 24 hours.
  • U.S. Pat. No. 4,769,318 to Hamasaki et al. is directed to additive solutions for blood preservation and activation.
  • U.S. Pat. No. 5,624,794 to Bitensky et al. and also U.S. Pat. No. 6,162,396 to Bitensky et al. are directed to the storage of red blood cells under oxygen-depleted conditions.
  • U.S. Pat. No. 5,789,151 to Bitensky et al. is directed to blood storage additive solutions.
  • Rejuvesol available from enCyte Corp., Braintree, Mass.
  • Rejuvesol is add to blood after cold storage (i.e., 4° C.) just prior to transfusion or prior to freezing (i.e., at ⁇ 80° C. with glycerol) for extended storage.
  • Another object of the present invention to provide a method for extending the storage of red blood cells using oxygen-free additive solutions and oxygen removal.
  • the present invention provides methods and compositions for extending the useful shelf-life of red blood cells.
  • the method of the invention comprises adding a metabolic supplement to packed red blood cells, adding an additive solution, preferably an oxygen-free additive solution, to said red blood cells, and storing said red blood cells at a temperature above freezing, preferably 4° C., under conditions of oxygen-depletion.
  • Metabolic supplement compositions of the invention comprise pyruvate, inosine, adenine, monobasic and dibasic phosphate salts at a pH from about 5 to about 8. Rejuvesol, or modification thereof, may be used as a metabolic supplement solution.
  • Oxygen depletion may be effected by flushing the red blood cells with an inert gas as described with oxygen depleted refrigerated storage in U.S. Pat. No. 5,624,794 and U.S. Pat. No. 6,162,396.
  • Preferred oxygen-free additive solutions comprise modifications of EAS61 (Hess et al., Transfusion 40: 1007-1011), and OFAS1 (U.S. Pat. No. 5,789,151).
  • a preferred oxygen-free additive solution is OFAS3.
  • the present invention extends the useful shelf life of refrigerated packed red blood cells from the current approximately 6 week limit to about 12 to about 20 weeks.
  • FIG. 1 shows the effect of pH and oxygen depletion on cellular ATP levels of red blood cells in OFAS3.
  • FIG. 2 shows the effect of pH and oxygen depletion on the percentage of red blood cells exposing phosphotidylserine in OFAS3.
  • FIG. 3 shows the effect of pH and oxygen depletion on red blood cell hemolysis in OFAS3.
  • FIG. 4 shows the effect on red blood cell ATP levels of metabolic supplements added at different pH's in the presence or absence of oxygen.
  • FIG. 5 shows the effect on red blood cell 2,3-DPG levels of metabolic supplements added at different pH's in the presence or absence of oxygen.
  • FIG. 6 shows the effect on the percentage of red blood cells exposing phosphotidylserine of addition of metabolic supplements at different pH's in the presence and absence of oxygen.
  • FIG. 7 shows the effect on vesicle protein production of red blood cells of addition of metabolic supplements at different pH's in the presence or absence of oxygen.
  • the present invention provides methods and compositions extending the useful shelf life of refrigerated red blood cells.
  • the present invention more than doubles the useful shelf life of red blood cells and overcomes current limitations in the blood industry by providing longer and less perishable blood supplies.
  • Metabolic supplementation is used commercially.
  • Rejuvesol is indicated for use at 37° C. and a 1 hour rejuvenation of stored blood just prior to transfusion or just prior to freezing in glycerol.
  • the present invention describes addition of metabolic supplement during refrigerated storage combined with the use of oxygen free additive solution (i.e., OFAS3) and oxygen removal.
  • OFAS3 oxygen free additive solution
  • unprecedented results have been obtained. For example, red blood cell storage well beyond the current 6-week limit for 12 or up to 20 weeks at 4° C. with levels of 2-3 DPG and ATP that are above those found in freshly drawn blood.
  • Metabolic supplementation of the invention is effected at least once, preferably during oxygen-depleted refrigerated storage (i.e., 4°) of red blood cells (c.f., U.S. Pat. Nos. 5,624,794; 6,162,396), along with oxygen-free additive solution, preferably OFAS3 or modification thereof. Blood units are not warmed. EAS61 and OFAS1 are additive solutions known in the art.
  • Metabolic supplement is added to refrigerated red blood cells. A first addition is made within 6-10 weeks of storage. A second addition is optionally added within 11-20 weeks of storage.
  • Metabolic supplement solution composition is presented in Table 1.
  • Table 1 Concentration Ingredient (g/unit of addition) Na pyruvate 0.1-2.0 Inosine 0.5-3.0 Adenine 0.01-1.0 Na phosphate dibasic 0-2.0 Na phosphate monobasic 0-2.0 pH 5.5-8.0
  • test units were stored in an anaerobic environment following multiple flushes to minimize the oxygen content of each unit using highly purified Ar and H 2 Following completion of sampling, the test units were made anaerobic following the procedure provided by the sponsor. Briefly, the units were transferred to a 2000 mL transfer bag using the SCD. Sputtering grade argon was introduced into the unit via a 0.22 micron filter until the transfer bag was completely filled with gas/blood and rotated 10 min at room temperature. Following this hold period, the gas was expelled through the same 0.22 micron filter using a plasma expressor and a vacuum line. This procedure was repeated 6 times, and the unit was transferred to a standard PL146 red cell storage bag with an Ar flush.
  • the unit was then placed in an anaerobic culture jar and 3 exchanges of the contents of the jar were performed with Ar, the last consisting of 2 parts Ar, 1 part scientific grade H 2 before the jar was placed in a monitored 4° C. refrigerator.
  • the storage jar again underwent gas replacement prior to the unit being placed back in the refrigerator.
  • Jars were flushed weekly with Ar if no sampling occurred in that week. Control units were stored in the same refrigerator without altering their gaseous environment.
  • test units After 7 weeks of storage, test units underwent a metabolic supplementation using a licensed solution (Rejuvesol, Cytosol Laboratories, Braintree, Mass.); test units underwent an additional metabolic supplementation at 11 weeks (if recoveries to date indicated that continued storage was warranted, vida infra)
  • the contents of the bottle of metabolic supplement were aspirated via needle and syringe and injected via a sampling port into a plastic transfer bag that had been previously flushed with Ar and to which had already been attached a 0.22 micron filter.
  • the solution was then transferred to the unit by sterile docking, and the unit was promptly returned to refrigerated storage (without repeating the gas exchange procedure and without incubation or washing).
  • Control units were utilized for radiolabeling and autologous reinfusion at 10 weeks; test units were continued in the protocol so long as the prior radiolabeled recovery suggested the continued viability of the cells.
  • the ATP must have been at least 50% of the Day 0 value, and the hemolysis must have been no more than 3.0% at the preceding sampling.
  • Radiolabe]ing to allow for determination or in vivo red cell recovery was conducted according to published procedures [J. Nucl. Med. 1975; 16:435-7] 10-20 ⁇ CiNa 2 51 CrO 4 (Bracco, Princeton, N.J.) were added to a 10 mL aliquot of the unit's cells for 30 min. at room temperature followed by a single double-volume saline wash.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Biophysics (AREA)
  • Physiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
US10/295,772 2001-11-16 2002-11-15 Method for extending the useful shelf-life of refrigerated red blood cells by nutrient supplementation Abandoned US20030153074A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US10/295,772 US20030153074A1 (en) 2001-11-16 2002-11-15 Method for extending the useful shelf-life of refrigerated red blood cells by nutrient supplementation
US11/138,135 US7723017B2 (en) 2001-11-16 2005-05-26 Method for extending the useful shelf-life of refrigerated red blood cells by nutrient supplementation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33240901P 2001-11-16 2001-11-16
US10/295,772 US20030153074A1 (en) 2001-11-16 2002-11-15 Method for extending the useful shelf-life of refrigerated red blood cells by nutrient supplementation

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/138,135 Continuation US7723017B2 (en) 2001-11-16 2005-05-26 Method for extending the useful shelf-life of refrigerated red blood cells by nutrient supplementation

Publications (1)

Publication Number Publication Date
US20030153074A1 true US20030153074A1 (en) 2003-08-14

Family

ID=23298102

Family Applications (2)

Application Number Title Priority Date Filing Date
US10/295,772 Abandoned US20030153074A1 (en) 2001-11-16 2002-11-15 Method for extending the useful shelf-life of refrigerated red blood cells by nutrient supplementation
US11/138,135 Expired - Lifetime US7723017B2 (en) 2001-11-16 2005-05-26 Method for extending the useful shelf-life of refrigerated red blood cells by nutrient supplementation

Family Applications After (1)

Application Number Title Priority Date Filing Date
US11/138,135 Expired - Lifetime US7723017B2 (en) 2001-11-16 2005-05-26 Method for extending the useful shelf-life of refrigerated red blood cells by nutrient supplementation

Country Status (6)

Country Link
US (2) US20030153074A1 (fr)
EP (1) EP1450604A4 (fr)
JP (1) JP2005535279A (fr)
AU (1) AU2002366082A1 (fr)
CA (1) CA2467223A1 (fr)
WO (1) WO2003043571A2 (fr)

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060086675A1 (en) * 2004-10-22 2006-04-27 Cryofacets, Inc. System, chamber, and method for fractionation and elutriation of fluids containing particulate components
US20060147895A1 (en) * 2004-10-22 2006-07-06 Cryofacets, Inc. System, chamber, and method for fractionation, elutriation, and decontamination of fluids containing cellular components
US20090239208A1 (en) * 2008-03-21 2009-09-24 Veronique Mayaudon Red Blood Cell Storage Medium For Extended Storage
US20110117647A1 (en) * 2008-03-21 2011-05-19 Fenwal, Inc. Red Blood Cell Storage Medium For Extended Storage
US9005343B2 (en) 2010-05-05 2015-04-14 New Health Sciences, Inc. Integrated leukocyte, oxygen and/or CO2 depletion, and plasma separation filter device
US20150147744A1 (en) * 2011-03-28 2015-05-28 New Health Sciences, Inc. Method and system for removing oxygen and carbon dioxide during red cell blood processing using an inert carrier gas and manifold assembly
US9315775B2 (en) 2011-03-16 2016-04-19 Mayo Foundation For Medical Education And Research Methods and materials for prolonging useful storage of red blood cell preparations and platelet preparations
US9339025B2 (en) 2010-08-25 2016-05-17 New Health Sciences, Inc. Method for enhancing red blood cell quality and survival during storage
US9409128B2 (en) 2009-10-23 2016-08-09 Fenwal, Inc. Methods for storing red blood cell products
US9801784B2 (en) 2015-04-23 2017-10-31 New Health Sciences, Inc. Anaerobic blood storage containers
US9844615B2 (en) 2009-10-12 2017-12-19 New Health Sciences, Inc. System for extended storage of red blood cells and methods of use
US9877476B2 (en) 2013-02-28 2018-01-30 New Health Sciences, Inc. Gas depletion and gas addition devices for blood treatment
US10058091B2 (en) 2015-03-10 2018-08-28 New Health Sciences, Inc. Oxygen reduction disposable kits, devices and methods of use thereof
US10136635B2 (en) 2010-05-05 2018-11-27 New Health Sciences, Inc. Irradiation of red blood cells and anaerobic storage
US10583192B2 (en) 2016-05-27 2020-03-10 New Health Sciences, Inc. Anaerobic blood storage and pathogen inactivation method
US11013771B2 (en) 2015-05-18 2021-05-25 Hemanext Inc. Methods for the storage of whole blood, and compositions thereof
US11284616B2 (en) 2010-05-05 2022-03-29 Hemanext Inc. Irradiation of red blood cells and anaerobic storage

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2467087A1 (fr) * 2001-11-16 2003-05-30 Hemanext, Llc Solution d'additif pour la conservation de sang
US8828226B2 (en) 2003-03-01 2014-09-09 The Trustees Of Boston University System for assessing the efficacy of stored red blood cells using microvascular networks
US20050074743A1 (en) * 2003-10-06 2005-04-07 Purmal Andrei A. Method and composition for treating a biological sample
US9314014B2 (en) 2004-02-18 2016-04-19 University Of Maryland, Baltimore Compositions and methods for the storage of red blood cells
EP2271209B1 (fr) 2008-03-27 2016-07-13 Biolife Solutions, Inc. Matériaux et procédés pour le prélévement hypothermique de sang total
BR112012008683B8 (pt) 2009-10-12 2022-11-08 Hemanext Inc dispositivo de armazenagem de sangue para armazenar sangue depletado de oxigênio e dióxido de carbono, dispositivo de depleção de oxigênio e dióxido de carbono, método para remover oxigênio e dióxido de carbono de hemácias, sistema de armazenagem de sangue, dispositivo de armazenagem de sangue, método para remover oxigênio de hemácias e método para aumentar os níveis de adenosina trifosfato (atp) nas hemácias
WO2011046963A1 (fr) 2009-10-12 2011-04-21 New Health Sciences, Inc. Dispositifs d'appauvrissement en oxygène et procédés pour retirer l'oxygène de globules rouges
WO2011083490A2 (fr) * 2009-12-31 2011-07-14 Intas Pharmaceuticals Limited Composition vétérinaire stable permettant une absorption améliorée du phosphore
WO2011103179A1 (fr) * 2010-02-16 2011-08-25 Viacell, Llc Compositions contenant de l'arginine et procédés de traitement des globules rouges
US9066909B2 (en) 2012-09-06 2015-06-30 Biomet Biologics, Llc Methods for producing and using rejuvenated red blood cells
US10253295B2 (en) 2012-09-06 2019-04-09 Biomet Manufacturing, Llc Methods for producing rejuvenated red blood cells
US9011408B2 (en) 2013-01-31 2015-04-21 Biomet Biologics, Llc Functionally-closed, sterile blood processing solution system and method
US9102918B2 (en) 2013-01-31 2015-08-11 Biomet Biologics, Llc Methods for rejuvenating red blood cells
US9103842B2 (en) 2013-01-31 2015-08-11 Biomet Biologics, Llc Methods for rejuvenating red blood cells

Citations (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2194775A (en) * 1937-09-22 1940-03-26 Donald Colvin Beverage dispenser and carbonator
US3359748A (en) * 1966-03-25 1967-12-26 Jack J Booth Slush co2 control
US3979029A (en) * 1974-12-16 1976-09-07 Hodgson R W Multi-directional aerosol valve for use on an aerosol container
US3995770A (en) * 1975-03-19 1976-12-07 Beatrice Foods Co. Apparatus for dispensing beverages
US4022031A (en) * 1973-05-02 1977-05-10 Calim Thomas F Method for producing frozen confection
US4201558A (en) * 1978-12-01 1980-05-06 Beatrice Foods Co. Method and apparatus for preparing and dispensing a semi-frozen product
US4440795A (en) * 1981-02-06 1984-04-03 Miller Brewing Company Process for the preparation of a stable citrus flavored malt beverage
US4448036A (en) * 1981-10-30 1984-05-15 Gerlach Industries Inc. Refrigeration system
US4769318A (en) * 1986-06-03 1988-09-06 Ube Industries, Ltd. Additive solution for blood preservation and activation
US4869396A (en) * 1987-08-24 1989-09-26 Kirin Beer Kabushiki Kaisha Draught beer dispensing system
US5304384A (en) * 1993-03-23 1994-04-19 Labatt Brewing Company Limited Improvements in production of fermented malt beverages
US5463877A (en) * 1994-07-14 1995-11-07 Wilch Manufacturing, Inc. Dispenser having edge-lighted, transparent valve for product display
US5615559A (en) * 1995-03-01 1997-04-01 Apv Crepaco Inc. Method and apparatus for recirculating product in a refrigeration system
US5624794A (en) * 1995-06-05 1997-04-29 The Regents Of The University Of California Method for extending the useful shelf-life of refrigerated red blood cells by flushing with inert gas
US5789151A (en) * 1997-05-15 1998-08-04 The Regents Of The University Of California Prolonged cold storage of red blood cells by oxygen removal and additive usage
US5869114A (en) * 1994-03-18 1999-02-09 Labatt Brewing Company Limited Production of fermented malt beverages
US6150085A (en) * 1998-09-16 2000-11-21 The United States Of America As Represented By The Secretary Of The Army Prolonged storage of red blood cells and composition
US6162396A (en) * 1997-04-26 2000-12-19 The Regents Of The University Of California Blood storage device and method for oxygen removal
US20020083730A1 (en) * 2000-11-01 2002-07-04 Giroux Mark S. Frozen beer product, method and apparatus

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996029864A1 (fr) * 1995-03-24 1996-10-03 Organ, Inc. Methode de rajeunissement de globules rouges perimes

Patent Citations (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2194775A (en) * 1937-09-22 1940-03-26 Donald Colvin Beverage dispenser and carbonator
US3359748A (en) * 1966-03-25 1967-12-26 Jack J Booth Slush co2 control
US4022031A (en) * 1973-05-02 1977-05-10 Calim Thomas F Method for producing frozen confection
US3979029A (en) * 1974-12-16 1976-09-07 Hodgson R W Multi-directional aerosol valve for use on an aerosol container
US3995770A (en) * 1975-03-19 1976-12-07 Beatrice Foods Co. Apparatus for dispensing beverages
US4201558A (en) * 1978-12-01 1980-05-06 Beatrice Foods Co. Method and apparatus for preparing and dispensing a semi-frozen product
US4440795A (en) * 1981-02-06 1984-04-03 Miller Brewing Company Process for the preparation of a stable citrus flavored malt beverage
US4448036A (en) * 1981-10-30 1984-05-15 Gerlach Industries Inc. Refrigeration system
US4769318A (en) * 1986-06-03 1988-09-06 Ube Industries, Ltd. Additive solution for blood preservation and activation
US4869396A (en) * 1987-08-24 1989-09-26 Kirin Beer Kabushiki Kaisha Draught beer dispensing system
US5304384A (en) * 1993-03-23 1994-04-19 Labatt Brewing Company Limited Improvements in production of fermented malt beverages
US5869114A (en) * 1994-03-18 1999-02-09 Labatt Brewing Company Limited Production of fermented malt beverages
US5463877A (en) * 1994-07-14 1995-11-07 Wilch Manufacturing, Inc. Dispenser having edge-lighted, transparent valve for product display
US5615559A (en) * 1995-03-01 1997-04-01 Apv Crepaco Inc. Method and apparatus for recirculating product in a refrigeration system
US5624794A (en) * 1995-06-05 1997-04-29 The Regents Of The University Of California Method for extending the useful shelf-life of refrigerated red blood cells by flushing with inert gas
US6162396A (en) * 1997-04-26 2000-12-19 The Regents Of The University Of California Blood storage device and method for oxygen removal
US5789151A (en) * 1997-05-15 1998-08-04 The Regents Of The University Of California Prolonged cold storage of red blood cells by oxygen removal and additive usage
US6150085A (en) * 1998-09-16 2000-11-21 The United States Of America As Represented By The Secretary Of The Army Prolonged storage of red blood cells and composition
US20020083730A1 (en) * 2000-11-01 2002-07-04 Giroux Mark S. Frozen beer product, method and apparatus

Cited By (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060086675A1 (en) * 2004-10-22 2006-04-27 Cryofacets, Inc. System, chamber, and method for fractionation and elutriation of fluids containing particulate components
US20060147895A1 (en) * 2004-10-22 2006-07-06 Cryofacets, Inc. System, chamber, and method for fractionation, elutriation, and decontamination of fluids containing cellular components
WO2007106666A2 (fr) * 2006-03-01 2007-09-20 Cryofacets, Inc. SYSTÈME, CHAMBRE, ET PROCÉDÉ de fractionnement, d'ELUTRIATION, et de décontamination de fluides contenant deS composants cellulaires
WO2007106666A3 (fr) * 2006-03-01 2007-12-06 Cryofacets Inc SYSTÈME, CHAMBRE, ET PROCÉDÉ de fractionnement, d'ELUTRIATION, et de décontamination de fluides contenant deS composants cellulaires
US20090239208A1 (en) * 2008-03-21 2009-09-24 Veronique Mayaudon Red Blood Cell Storage Medium For Extended Storage
US20110117647A1 (en) * 2008-03-21 2011-05-19 Fenwal, Inc. Red Blood Cell Storage Medium For Extended Storage
US8871434B2 (en) 2008-03-21 2014-10-28 Fenwal, Inc. Red blood cell storage medium for extended storage
US8968992B2 (en) 2008-03-21 2015-03-03 Fenwal, Inc. Red blood cell storage medium for extended storage
US11433164B2 (en) 2009-10-12 2022-09-06 Hemanext Inc. System for extended storage of red blood cells and methods of use
US9844615B2 (en) 2009-10-12 2017-12-19 New Health Sciences, Inc. System for extended storage of red blood cells and methods of use
US10603417B2 (en) 2009-10-12 2020-03-31 Hemanext Inc. System for extended storage of red blood cells and methods of use
US11864553B2 (en) 2009-10-23 2024-01-09 Fenwal, Inc. Methods and systems for providing red blood cell products with reduced plasma
US9943077B2 (en) 2009-10-23 2018-04-17 Fenwal, Inc. Methods for storing red blood cell products
US9409128B2 (en) 2009-10-23 2016-08-09 Fenwal, Inc. Methods for storing red blood cell products
US10136635B2 (en) 2010-05-05 2018-11-27 New Health Sciences, Inc. Irradiation of red blood cells and anaerobic storage
US9005343B2 (en) 2010-05-05 2015-04-14 New Health Sciences, Inc. Integrated leukocyte, oxygen and/or CO2 depletion, and plasma separation filter device
US9539375B2 (en) 2010-05-05 2017-01-10 New Health Sciences, Inc. Integrated leukocyte, oxygen and/or CO2 depletion, and plasma separation filter device
US11284616B2 (en) 2010-05-05 2022-03-29 Hemanext Inc. Irradiation of red blood cells and anaerobic storage
US10065134B2 (en) 2010-05-05 2018-09-04 New Health Sciences, Inc. Integrated leukocyte, oxygen and/or CO2 depletion, and plasma separation filter device
US9339025B2 (en) 2010-08-25 2016-05-17 New Health Sciences, Inc. Method for enhancing red blood cell quality and survival during storage
US10251387B2 (en) 2010-08-25 2019-04-09 New Health Sciences, Inc. Method for enhancing red blood cell quality and survival during storage
US9315775B2 (en) 2011-03-16 2016-04-19 Mayo Foundation For Medical Education And Research Methods and materials for prolonging useful storage of red blood cell preparations and platelet preparations
US20150147744A1 (en) * 2011-03-28 2015-05-28 New Health Sciences, Inc. Method and system for removing oxygen and carbon dioxide during red cell blood processing using an inert carrier gas and manifold assembly
US9067004B2 (en) 2011-03-28 2015-06-30 New Health Sciences, Inc. Method and system for removing oxygen and carbon dioxide during red cell blood processing using an inert carrier gas and manifold assembly
US9968718B2 (en) * 2011-03-28 2018-05-15 New Health Sciences, Inc. Method and system for removing oxygen and carbon dioxide during red cell blood processing using an inert carrier gas and manifold assembly
US9877476B2 (en) 2013-02-28 2018-01-30 New Health Sciences, Inc. Gas depletion and gas addition devices for blood treatment
US10687526B2 (en) 2013-02-28 2020-06-23 Hemanext Inc. Gas depletion and gas addition devices for blood treatment
US11375709B2 (en) 2015-03-10 2022-07-05 Hemanext Inc. Oxygen reduction disposable kits, devices and methods of use thereof
US11350626B2 (en) 2015-03-10 2022-06-07 Hemanext Inc. Oxygen reduction disposable kits, devices and methods of use thereof (ORDKit)
US10058091B2 (en) 2015-03-10 2018-08-28 New Health Sciences, Inc. Oxygen reduction disposable kits, devices and methods of use thereof
US11638421B2 (en) 2015-03-10 2023-05-02 Hemanext Inc. Oxygen reduction disposable kits, devices and methods of use thereof
US10849824B2 (en) 2015-04-23 2020-12-01 Hemanext Inc. Anaerobic blood storage containers
US9801784B2 (en) 2015-04-23 2017-10-31 New Health Sciences, Inc. Anaerobic blood storage containers
US11013771B2 (en) 2015-05-18 2021-05-25 Hemanext Inc. Methods for the storage of whole blood, and compositions thereof
US11147876B2 (en) 2016-05-27 2021-10-19 Hemanext Inc. Anaerobic blood storage and pathogen inactivation method
US10583192B2 (en) 2016-05-27 2020-03-10 New Health Sciences, Inc. Anaerobic blood storage and pathogen inactivation method
US11911471B2 (en) 2016-05-27 2024-02-27 Hemanext Inc. Anaerobic blood storage and pathogen inactivation method

Also Published As

Publication number Publication date
WO2003043571A3 (fr) 2004-03-18
US20050277108A1 (en) 2005-12-15
US7723017B2 (en) 2010-05-25
AU2002366082A8 (en) 2003-06-10
EP1450604A2 (fr) 2004-09-01
CA2467223A1 (fr) 2003-05-30
AU2002366082A1 (en) 2003-06-10
EP1450604A4 (fr) 2005-01-12
JP2005535279A (ja) 2005-11-24
WO2003043571A2 (fr) 2003-05-30

Similar Documents

Publication Publication Date Title
US7723017B2 (en) Method for extending the useful shelf-life of refrigerated red blood cells by nutrient supplementation
US8071282B2 (en) Method of storing red blood cells with an acidic additive solution under oxygen depletion
US6447987B1 (en) Prolonged storage of red blood cells
Rubinstein et al. Processing and cryopreservation of placental/umbilical cord blood for unrelated bone marrow reconstitution.
US5955257A (en) Infusible grade short-term cell storage medium for mononuclear cells
FI86253C (fi) Syntetiskt, plasmafritt, transfusibelt foervaringsmedium foer blodplaettar.
US6150085A (en) Prolonged storage of red blood cells and composition
US8871434B2 (en) Red blood cell storage medium for extended storage
RU2396748C2 (ru) Среда для хранения клеток
US20120129148A1 (en) Compositions and methods for the storage of red blood cells
JP3069613B2 (ja) 血球のatpおよび2,3dpg濃度を長時間維持する赤血球の保存方法
Yoshida et al. The effects of additive solution pH and metabolic rejuvenation on anaerobic storage of red cells
US10897891B2 (en) Compositions and methods for prolonged cell storage
US8968992B2 (en) Red blood cell storage medium for extended storage
CA2598366A1 (fr) Compositions et procedes pour le stockage de globules rouges
EP1109447B1 (fr) Stockage prolonge de globules rouges
WO1996029864A1 (fr) Methode de rajeunissement de globules rouges perimes
Branch et al. Hematopoietic progenitor cells are resistant to dimethyl sulfoxide toxicity
Bode et al. Extended Storage of Platelets in an Artificial Medium with the Platelet Activation Inhibitors Prostaglandin E (1) and Theophylline
Torlontano et al. Evaluation of four different methods for platelet freezing
US20180184643A1 (en) Injectable preserving medium for preserving cells from placental blood, from bone marrow and from peripheral blood
US20220354108A1 (en) Preservation methods using trehalose with other cryoprotectants being absent from the cryopreservation protocol
Rathbun et al. Posttransfusion survival of red cells frozen for 8 weeks after 42‐day liquid storage in AS‐3
WO2002023988A1 (fr) Stockage prolonge de globules rouges
RU2782185C1 (ru) Добавочный раствор для хранения отмытых размороженных эритроцитов

Legal Events

Date Code Title Description
AS Assignment

Owner name: HEMANEXT, LLC, MARYLAND

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:BITENSKY, MARK W.;YOSHIDA, TATSURO;REEL/FRAME:014071/0835

Effective date: 20030506

STCB Information on status: application discontinuation

Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION

AS Assignment

Owner name: THE TRUSTEES OF BOSTON UNIVERSITY, MASSACHUSETTS

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:HEMANEXT HOLDINGS, LLC;REEL/FRAME:020098/0595

Effective date: 20070315

Owner name: THE TRUSTEES OF BOSTON UNIVERSITY,MASSACHUSETTS

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:HEMANEXT HOLDINGS, LLC;REEL/FRAME:020098/0595

Effective date: 20070315

AS Assignment

Owner name: NAVY, SECRETARY OF THE UNITED STATES OF AMERICA,VI

Free format text: CONFIRMATORY LICENSE;ASSIGNOR:BOSTON UNIVERSITY;REEL/FRAME:024191/0612

Effective date: 20091021