UA99481C2 - Process for production of recombinant human proinsulin c-peptide - Google Patents

Process for production of recombinant human proinsulin c-peptide

Info

Publication number
UA99481C2
UA99481C2 UAA201004806A UAA201004806A UA99481C2 UA 99481 C2 UA99481 C2 UA 99481C2 UA A201004806 A UAA201004806 A UA A201004806A UA A201004806 A UAA201004806 A UA A201004806A UA 99481 C2 UA99481 C2 UA 99481C2
Authority
UA
Ukraine
Prior art keywords
peptide
production
recombinant human
carbamide
hybrid protein
Prior art date
Application number
UAA201004806A
Other languages
Russian (ru)
Ukrainian (uk)
Inventor
Пьотр Івановіч Родіонов
Пьотр Пєтровіч Родіонов
Алєксєй Вячєславовіч Стєпанов
Вадім Васільєвіч Шматченко
Алєксандр Ніколаєвіч Байдусь
Ніколай Вікторовіч Борісов
Original Assignee
Общество С Ограниченной Ответственностью "Герофарм"
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Общество С Ограниченной Ответственностью "Герофарм" filed Critical Общество С Ограниченной Ответственностью "Герофарм"
Publication of UA99481C2 publication Critical patent/UA99481C2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention relates to the process for production of recombinant human c-peptide, comprising cultivation of strain producer Escherichia coli, destruction of bacterial cells by disintegration, separation of inclusion bodies containing hybrid protein, theirs solubilisation in buffer solution containing carbamide and dithiothreitol, renaturation and purification of hybrid protein, its degradation with trypsin and carboxypeptidase B, which is carried out simultaneously, wherein tryptic cleavage products are separated with chromatography on SP- sepharose, balanced with 0.03-0.1 M of ammonium-acetate buffer pH 3.6, containing 3 M of carbamide, eluated with linear gradient of potassium chloride from 0 to 0.5 M in initial buffer, with subsequent purifying and receiving of desired product.
UAA201004806A 2007-09-24 2008-09-23 Process for production of recombinant human proinsulin c-peptide UA99481C2 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU2007135323/10A RU2451750C2 (en) 2007-09-24 2007-09-24 Method of producing recombinant human proinsulin c-peptide

Publications (1)

Publication Number Publication Date
UA99481C2 true UA99481C2 (en) 2012-08-27

Family

ID=40511663

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA201004806A UA99481C2 (en) 2007-09-24 2008-09-23 Process for production of recombinant human proinsulin c-peptide

Country Status (4)

Country Link
EA (1) EA201070372A1 (en)
RU (1) RU2451750C2 (en)
UA (1) UA99481C2 (en)
WO (1) WO2009041858A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150315259A1 (en) * 2012-03-19 2015-11-05 Madeleine Phamaceuticals Pty Ltd Method of producing a recombinant peptide
RU2590781C1 (en) * 2015-03-04 2016-07-10 Открытое акционерное общество "Уралэлектромедь" Method of extracting antimony and lead

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19735711C2 (en) * 1997-08-18 2001-04-26 Aventis Pharma Gmbh Process for the preparation of a precursor to insulin or insulin derivatives with correctly linked cystine bridges
RU2144082C1 (en) * 1998-07-23 2000-01-10 Навашин Петр Сергеевич Recombinant plasmid encoding fused protein-precursor of human insulin (variants), strain of bacterium escherichia coli - producer of fused protein-precursor of human insulin (variants), method of human insulin preparing
RU2144957C1 (en) * 1999-02-26 2000-01-27 Институт биоорганической химии им.М.М.Шемякина и Ю.А.Овчинникова РАН Recombinant plasmid dna ppins07 encoding fused polypeptide containing human proinsulin and strain of bacterium escherichia coli - producer of fused polypeptide containing human proinsulin
RU2141531C1 (en) * 1999-05-26 1999-11-20 Российское акционерное общество открытого типа "Биопрепарат" Method of recombinant human insulin preparing
RU2208637C1 (en) * 2002-04-16 2003-07-20 Институт биоорганической химии им. М.М. Шемякина и Ю.А. Овчинникова РАН Method for preparing gene-engineering human insulin
RU2232813C1 (en) * 2003-02-18 2004-07-20 Открытое акционерное общество "Национальные биотехнологии" Method for industrial preparing human recombinant insulin

Also Published As

Publication number Publication date
RU2007135323A (en) 2009-03-27
EA201070372A1 (en) 2010-10-29
WO2009041858A1 (en) 2009-04-02
RU2451750C2 (en) 2012-05-27

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