UA156438U - METHOD OF EXTRACTION OF EXTRACTION ENZYMES - Google Patents

Abstract

The method of extracting rennet enzymes includes the use of a hydrochloric acid extractant. Additionally, a 20% solution of acetic acid is injected.

Description

The useful model belongs to biotechnology, directly to the food industry, and can be used for the production of rennet enzymes used in the technology of fermented milk products.

Rennet enzymes play an important role in cheese production. Almost until the end of the 20th century, the dairy industry did not know other coagulants. In cheesemaking technology, rennet enzymes perform two main functions: chymosin directly splits kappa-casein, which initiates the coagulation of milk and the cheese-making process, and also causes hydrolysis reactions of milk proteins, which are necessary for the formation of the taste and aroma of the finished product. Moreover, rennet enzymes affect the ripening process, as they retain some of their original properties in cheeses.

Renal enzymes are obtained by the extraction method from the rennet of calves, lambs, goats, which consumed only milk and dairy products. The rennet enzyme extract is purified by filtration. The activity of rennet enzyme depends on the factors of the technological conditions of their production from rennet (1-21.

The prototype of a useful model is the repeated extraction of rennet enzymes from crushed rennet of calves and lambs using a solution of sodium chloride with a sodium benzoate content of 0.1-3 95

ISI

Disadvantages of the analogue include the fact that this method requires a significant number of repetitions, with this method the output of the finished product is lower, which has a high production cost. The presence of sodium benzoate impairs the naturalness of the product.

The basis of a useful model is the task of developing a method of extracting rennet enzymes by using hydrochloric and acetic acid solutions as extractants, which will ensure an increase in the extraction of rennet enzymes and the content of total protein, an increase in the shelf life, without losing its activity.

The task is solved by the fact that the method of extracting rennet enzymes includes the use of a hydrochloric acid extractant. In addition, a 20% solution of acetic acid is injected.

For this purpose, 2 groups of samples were formed: control and experimental (Table 1). In the control group of samples, extraction from 0.2 g of rennet homogenate was performed by adding 10.0 cm3 of distilled water and 0.8 cm3 of EN hydrochloric acid solution with constant stirring.

In the experimental group of samples, rennet enzymes were extracted from 0.2 g of rennet homogenate by adding 10.0 cm? of distilled water, 0.8 cm3 of 6N hydrochloric acid solution and 0.15 cm3 of acetic acid with constant stirring.

Table 1

Scheme of the experiment

Volume of solution Volume Volume of rennet solution in one sample, g of sample, cm of water, cm of acid, cm

Control. | 2-02 7 | 7777/0877 |1711117100 Her (Dosldna | - 02/77/0687 |77117100 | 05

After the extraction, which lasted 12 hours, the protein content in the solutions was determined (Table 2).

Table 2

Extraction indicators of rennet enzymes by protein content

Note! P«0.001

According to the results of the experiments, it was established that with the introduction of acetic acid solution, the content of total protein, including rennet enzymes, was 2.23 times higher than in the control.

Immobilized rennet enzymes have a prolonged shelf life and do not lose their activity. The drug is easily transported and does not require special storage conditions, it ferments milk even under mesophilic conditions.

Adding acetic acid to the extractant has a positive effect on the extraction of digestive enzymes from the whey homogenate.

The method has wide industrial significance because the use of the proposed extractant allows to increase the yield of the finished product per unit mass of biomaterial (whey homogenate).

The proposed method makes it possible to increase the yield of finished products from a unit mass of rennet, reduce the cost of the extraction process, and make the product as natural as possible.

Sources of information: 1. Meeiat Sygypo, Zitapia Vau, Zschshara Voze, apa MimekKk Nai. Vgoadeg Miem/: Mystobia! Epgutev apa Tneig Reiemapse ip Ipdivigiez5, Meadisipe, apa Veuopa. Wiomey Wezeagsy Ipiegpaiyopa!. 11 Ber. 2013. R. 1-18. OVI: pyrz//aoii.ogo/10.1155/2013/329121. 2. Karpenko O.V. Scientific principles of creation of biotechnologies of surface-active substances with polyfunctional properties: autoref. thesis for the acquisition of sciences, the degree of Dr. Tech. Sciences: specialist 03.00.20 "Biotechnology". Kyiv, 2015. 44 p. 3. Method of production of milk-coagulant enzyme preparation: pat. 2467068 (YOU); statement 2011-05-25; publication 20.11.2012.

Claims (1)

USEFUL MODEL FORMULA The method of extracting rennet enzymes, which includes the use of a hydrochloric acid extractant, which is distinguished by the fact that a 2095 solution of acetic acid is additionally introduced.