UA137448U - METHOD OF EXTRACTION OF CURRENT ENZYMES - Google Patents

Abstract

The method of extraction of rennet enzymes involves the use of extractants. The extractant contains a solution of lactic acid.

Description

The useful model belongs to biotechnology, directly to the food industry, and can be used for the production of rennet enzymes used in the technology of fermented milk products.

Rennet enzymes play an important role in cheese production. Almost until the end of the 20th century, the dairy industry did not know other coagulants. In the cheese-making technology, rennet enzymes perform two main functions: chymosin directly splits kappa-casein, which initiates the coagulation of milk and the cheese-making process, and also causes hydrolysis reactions of milk proteins, which are necessary for the formation of the taste and aroma of the finished product. Moreover, rennet enzymes affect the ripening process, as they retain some of their original properties in cheeses.

Renal enzymes are obtained by the extraction method from the rennet of calves, lambs, goats that consumed only milk and dairy products. The rennet enzyme extract is purified by filtration. The activity of rennet enzymes depends on the factors of the technological conditions of their production from rennet (1-21.

The closest analogue is the repeated extraction of rennet enzymes from crushed rennet of calves and lambs using a sodium chloride solution with a sodium benzoate content of 0.1-3%.

The disadvantages of the closest analogue include the fact that this method requires a significant number of repetitions, with this method the output of the finished product is lower, which has a high cost of production. The presence of sodium benzoate impairs the naturalness of the product.

The method proposed by us allows to increase the yield of finished products from a unit mass of rennet, to reduce the cost of the extraction process, and to make the product as natural as possible.

The basis of a useful model is the task of developing a method of extracting rennet enzymes by using hydrochloric and lactic acid solutions as extractants.

The task is solved in the method of extracting rennet enzymes, which includes the use of extractants, according to a useful model, the extractant contains a solution of lactic acid.

For this purpose, 2 groups of samples were formed: control and experimental (Table 1). In the control group of samples, extraction from 0.2 g of whey homogenate was performed by adding 10.0 cm3 of distilled water and 0.8 cm" of hydrochloric acid solution with constant stirring. In

From the experimental group of samples, rennet enzymes were extracted from 0.2 g of rennet homogenate by adding 10.0 cm3 of distilled water, 0.8 cm3 of 6H hydrochloric acid solution, and 0.25 cm3 of lactic acid with constant stirring.

Table 1

Scheme of the experiment. Volume of solution Volume Volume of solution in one sample, g and sample, cm of water, cm of acid, cm

Control | 77/02 7777 77771008 17111100 | 7-7 (Dosldna. | 02 g | --(08.ЮюЮБ| 5 Byu .yuKhslob0 | 025

After the extraction, which lasted 12 hours, the protein content in the solutions was determined (Table 2).

Table 2

Extraction indicators of rennet enzymes by protein content

Note: P-0.001.

According to the results of the experiments, it was established that with the introduction of lactic acid solution, the content of total protein, including rennet enzymes, was 2.07 times higher than in the control.

Thus, the research results indicate that the addition of lactic acid to the extractant has a positive effect on the extraction of digestive enzymes from whey homogenate.

The wide production value of the proposed method of extracting rennet enzymes is obvious, since the use of the proposed extractant allows to increase the yield of the finished product per unit mass of biomaterial (rennet homogenate).

The proposed method fits into the production technology of rennet enzymes.

The use of the proposed technique is of economic importance.

Sources of information: 1. Sigapu M. A Vgoadeg Miyem/: Mystobia! Epgutez apa TNeig VeYiemapse ip Ipdyvilev, Medaisipe, apa Veuopa //M. Sygypo, 5. Nau, 5. Voze |ei. a!) /Vioted. Rev. Ipi.-2013. - Mo!. 2013. -R. 1-18. 2. Karpenko O.V. Scientific principles of creation of biotechnologies of surface-active substances with polyfunctional properties: autoref. thesis for obtaining sciences. doctor's degree technical Sciences: specialist 03.00.20 "Biotechnology" /0.V. Karpenko. - K., 2015. - 44 p. 3. Elchaninov V.V., Koval A.D. The method of producing a milk-clotting enzyme preparation is a patent of the Russian Federation. Mo. 2467068, 2012, 3-5 p.

Claims (1)

USEFUL MODEL FORMULA A method of extracting rennet enzymes, which includes the use of extractants, which is characterized by the fact that the extractant contains a solution of lactic acid.