UA145469U - COLLECTION OF MEDICINAL PLANTS № 4 FOR PREVENTION AND TREATMENT OF TYPE 2 DIABETES MELLITUS - Google Patents

Abstract

Collection of medicinal plants for the prevention and treatment of type 2 diabetes, including rose hips. Additionally, it contains burdock roots, wheatgrass rhizomes, corn columns with receivers and cumin flowers, and all ingredients are taken in the following ratio, wt. h .: burdock roots 5 wheatgrass rhizomes 5 corn columns with receptacles 4 cumin flowers 3 rose hips fruits 2

Description

The useful model belongs to pharmacy and medicine, namely medicinal herbal preparations, and can be used for the prevention and treatment of type 2 diabetes, both independently and as part of complex therapy.

A well-known collection of medicinal plants "Arfazetin" for the prevention and treatment of type 2 diabetes, which includes blueberry leaves, broad beans, Eleutherococcus rhizomes and roots, rose hips, horsetail, St. John's wort, chamomile flowers (1). This plant collection is used for the prevention and treatment of type 2 diabetes patients in the form of a decoction, which is administered orally in 100 ml 2-3 times a day 30 minutes before meals.

The disadvantage of the known collection is insufficient pharmacological activity, resulting from the limited content of pharmacologically active substances of plant origin (with antioxidant, hepatoprotective, cardioprotective effects and its undesirable use, in particular during pregnancy, with hypertension, after myocardial infarction and in pediatric practice, due to the presence of rhizomes and roots of Eleutherococcus.

The basis of a useful model is the task of improving a well-known plant collection, in which by changing the composition of its ingredients aimed at increasing the content of biologically active substances Kk! hypoglycemic, hypolipidemic, antioxidant, anti-inflammatory, hepatoprotective, cardioprotective, gastroprotective effect, which, in turn, leads to an increase in the detoxification capacity of the body in the treatment of patients with type 2 diabetes, and therefore to an increase in the pharmacological activity of the collection as a whole. The composition of the declared plant collection includes: 1. Burdock roots containing the polysaccharide inulin, which exhibits hypoglycemic, anticholesterolemic and detoxification activity; fatty acids that regulate lipid metabolism exhibit cardioprotective, neuroprotective and immunomodulating activity. 2. Pyrium rhizomes containing polysaccharides, which exhibit hypoglycemic, anticholesterolemic and detoxification activity; ascorbic acid; carotenoids; saponins, which cause hepatoprotective, choleretic, diuretic, anti-inflammatory activity, and also affect metabolic processes in the body. 3. Corn cobs with capsules containing vitamins K, C, Vy, Bhe, Vz, B", Bhe, E, O; fat

From acid; flavonoids; tannins of the pyrocatechin group, which exhibit choleretic, hemostatic and hypoglycemic effects; increase the secretion of bile, reduce its viscosity, reduce the content of bilirubin, increase the content of prothrombin in the blood and accelerate its coagulation, contribute to the excretion of urates, oxalates, phosphates from the urine. 4. Cumin flowers containing flavonoids, flavonoid glycosides, tannins, which have choleretic, anti-inflammatory, antibacterial and antispasmodic effects, stimulate the secretory function of the stomach and pancreas, increase diuresis. 5. Rose hips are fruits that contain a significant amount of ascorbic acid; polyphenol compounds; vitamins

В, В», В5, РР, Е, К, which have an anti-sclerotic, anti-inflammatory effect; activate enzyme systems and redox processes in the body, normalize carbohydrate metabolism, increase the body's resistance to adverse environmental factors, increase bile secretion, increase diuresis, reduce capillary fragility and permeability.

Based on the above, the task is solved by the fact that the collection of medicinal plants Mo 4 for the prevention and treatment of type 2 diabetes, which includes rose hips, according to a useful model, additionally contains burdock roots, wheatgrass rhizomes, corn cobs and cumin flowers, and all ingredients are taken in the following ratio, mass. h.: burdock roots 5 wheat rhizomes 5 corn stalks WITH A receptacles of cumin flowers From rose hips fruits 2.

The declared fee is prepared as follows:

Medicinal plant raw materials collected and dried according to regulatory rules of procurement are weighed in the following amount, based on 100 g of the finished product, namely: burdock roots 26.32 wheatgrass rhizomes 26.32 corn columns 3 21405 scoops

cumin flowers 15.79 rose hips fruits 10.53.

Grind suspended materials to the appropriate particle size: flowers, grass, leaves - up to 5 mm; rhizome and roots - up to 3 mm. The crushed ingredients are sieved through a sieve with a hole size of 0.2 mm. The mixing of plant components is performed on a sheet of paper, in a wide mortar or porcelain cup using a spatula or spatula. First, plant materials prescribed in smaller quantities are mixed, then gradually added in larger quantities.

For the purpose of prevention and treatment of type 2 diabetes mellitus, the collection is used in the form of an aqueous decoction at a ratio of 1:10. To do this, take 20 g of the collection, fill it with 240 ml of water (taking into account the water absorption coefficient Kv-2), heat it in a boiling water bath for 30 minutes, then cool it at room temperature for 10 minutes, filter and squeeze the raw materials, add water, bringing the decoction to 200 ml. The specified phytoremedial agent is taken internally in 100 ml 2-3 times a day 30 minutes before meals.

A useful model is illustrated by examples:

Example 1. The quantitative content and qualitative composition of the declared herbal antidiabetic collection Mo 4 were studied for the content of biologically active substances and minerals that cause pharmacological activity. The results are given in Tables 1 and 2.

Determining the qualitative composition and establishing the quantitative content of flavonoids and hydroxycinnamic acids in the declared herbal antidiabetic collection was carried out by the method of high-performance liquid chromatography using the Adiyepi liquid chromatograph

Tesppoiodiyev5 1200 (USA), equipped with a diode-matrix detector |2, ЗИ.

As a result of the study, 4 types of flavonoids and 7 types of hydroxycinnamic acids were identified and their quantitative content was determined in the declared plant antidiabetic collection Mo 4. Among the flavonoids in the largest amount were isoquercetin 0.63 95 and luteolin 0.42 95, among hydroxycinnamic acids - quinic acid 1.5 95 (Table 1).

Table 1

Quantitative content of biologically active substances in the declared herbal antidiabetic composition

Mo 4

Mo. and and . 4 |luteolin.//////////7// | 042 |Cinnamon.//////// | 006 Sh 57777711 |Syrinova.7//// | 7 005 ЩЩ ( 6 77777771 | Ferulova.//////////// | 001ЩЩЦ 71111111 Kumarova.///////// | 02

The study of the mineral composition and determination of its quantitative content was carried out by the method of atomic absorption spectroscopy with atomization in an air-acetylene flame using an atomic absorption spectrophotometer C-115 PC |41.

Table 2

The content of macro- and microelements in the declared herbal antidiabetic composition Mo 4

Note: n/a - not detected

Coo)

As a result of the study, 5 macro- and C-microelements were identified and their quantitative content was determined in the declared herbal antidiabetic composition Mo 4 (Table 2).

Example 2. Determination of the antihyperglycemic activity of the declared herbal antidiabetic collection Mo 4 on rats with normal glucose homeostasis using an oral glucose tolerance test in comparison with the collection "Arfazetin" and metformin tablets.

The research was conducted on white outbred male rats weighing 180-200 g, which were kept in the vivarium nursery of the Central Research Laboratory of Ternopil National Medical University named after Y. Gorbachev, Ministry of Health of Ukraine, in accordance with sanitary and hygienic standards. All experiments were carried out in compliance with general ethical principles for conducting experiments on animals |5). The declared herbal antidiabetic collection Mo 4 was administered to rats for the purpose of prevention for 20 days intragastrically and in 1 hour. before carrying out a loading test with carbohydrates in the form of water decoctions (1:10) in doses of 6, 9, 12 ml/kg in comparison with the preparation "Arfazetin" (PrAT "Viola", Ukraine) (1:20) in a dose of 9 ml/kg , and metformin suspension SANDOZF (Lek SA, Poland) at a dose of 60 mg/kg.

The obtained experimental data were processed using the standard package of statistical programs "Ziaiviisa 6.0", the differences were considered statistically significant at p-b0.05.

Oral glucose tolerance test was performed in the morning on an empty stomach by intragastric administration of 40 95 glucose solution at a dose of 3 g/kg body weight.

The glucose content in the blood of the animals was determined immediately after the introduction of the studied agents and 15, 30, 60, 120 minutes after the loading test |б| by the glucose oxidase method with the help of test kits "cSlyukose-D" of the company "Filisit-Diagnostika" (Ukraine).

The hypoglycemic activity was evidenced by the ability of the stated plant collection and comparison drugs to reduce the level of glycemia at the 30th minute of the test (at the time of the maximum rise in the level of glucose in the blood of experimental rats in response to an oral carbohydrate load).

The obtained data are presented in Table 3.

Table C

Antihyperglycemic activity of the declared herbal antidiabetic collection Mo 4 during 20-day prophylactic administration to normoglycemic rats in comparison with the collection "Arfazetin" and metformin tablets under the conditions of the oral glucose tolerance test (teapeoE, p-8) 60 mg/kg

Mo 4.6 ml/kg

Mo 4.9 ml/kg

Mo 4, 12 ml/kg

Notes: 1. 7 - the differences are statistically significant in relation to the values of the intact group, p«e0.05 (according to O-criteria

Mann-Whitney); 2.7 - statistically significant differences with respect to the values of the comparison drug of the collection "Arfazetin", p«0.05 (according to Mann-Whittney O-criteria);

Z. n - the number of animals in each group.

The results of the study showed that the declared herbal antidiabetic collection Mo 4 in a dose of 12 ml/kg exhibits pronounced reliable antihyperglycemic activity relative to the intact group and the comparison drug of the collection "Arfazetin". By the ability to reduce alimentary hyperglycemia caused by exercise during the oral tolerance test to

From glucose, the antidiabetic collection Mo 4 in a dose of 12 ml/kg is superior to the well-known collection "Arfazetin" (table.

WITH).

The obtained data are natural, since the composition of the collection includes medicinal plant raw materials that exhibit hypoglycemic properties.

Burdock roots (Agsii gadih) and corn stalks with receptions (Mauaiv 5iuie sit 5idptaiyiv) are widely used in prescriptions of folk medicine for the prevention and treatment of type 2 diabetes, because they have established hypoglycemic activity. Wheatgrass rhizomes (Eguigidiae gppigote) contain polysaccharide complexes of the fructose group, which include inulin, which contributes to lowering the level of glucose, cholesterol and triglycerides in the blood, and the removal of radionuclides and ketone bodies. Cumin flowers (NeiisNguzi agepaiiii Pogez5) stimulate the secretory function of the stomach and pancreas, and also promote bile secretion. Due to the high content of vitamins and biologically active substances, rosehip fruits (Vosaye ytisii5) restore the body's antioxidant functions, affect carbohydrate metabolism, stimulate the immune system, and have anti-inflammatory activity (7.

Example 3. Determination of the antihyperglycemic activity of the declared plant antidiabetic collection Mo 4 on the model of diabetes caused by the introduction of dexamethasone.

Experimental diabetes, characterized by primary insulin resistance, impaired glucose tolerance and the development of metabolic syndrome, was modeled by subcutaneous administration of dexamethasone solution (KAKA, Slovenia) 0.125 mg/kg during 15 days of IEI. The research was carried out on white outbred sexually mature male rats weighing 200-220 g, which were kept in the vivarium nursery of the Central Research Laboratory of Ternopil National Medical University named after I.Ya.

Gorbachev of the Ministry of Health of Ukraine" in accordance with sanitary and hygienic norms. All experiments were carried out in compliance with general ethical principles regarding the conduct of experiments on animals |5). As comparison drugs, an aqueous decoction of the official herbal collection "Arfazetin" (PJSC "Viola", Ukraine) was used (1 :20) in a dose of 9 ml/kg and metformin suspension SANDOZF (Lek SA, Poland) in a dose of 60 mg/kg.

A decoction of herbal antidiabetic collection Mo 4 (1:10) at a dose of 12 ml/kg was administered once a day intragastrically every day for 15 days simultaneously with dexamethasone injections.

Control animals received water in a similar manner.

The state of glucose homeostasis of animals against the background of model pathology was assessed by the level of basal glycemia and glucose tolerance, which was determined using

From the intraperitoneal glucose tolerance test, which was performed as follows: after overnight fasting (4-6 p.m.), rats were injected intraperitoneally with a glucose solution at a dose of 2 g/kg in the morning. The glucose content in the blood of animals was determined before the introduction of glucose and 15, 45, 60 minutes after the loading test Ib| by the glucose oxidase method with the help of test kits "Glucose-D" of the company "Filisit-Diagnostika" (Ukraine).

The antihyperglycemic activity of the declared plant collection Mo 4 and comparison drugs was assessed by their ability to reduce hyperglycemia caused by a glucose load at the 15th minute of the test (at the time of the maximum rise in the level of glucose in the blood of animals in response to an intraperitoneal carbohydrate load).

As a result of the administration of dexamethasone to animals from the group of control pathology, the development of moderate hyperglycemia was observed, which is characteristic of the state of prediabetes or metabolic syndrome. Diabetes caused by the administration of excessive doses of glucocorticosteroids allows to reproduce the main pathogenetic mechanisms (disruption of insulin secretion and action) observed in patients with type 2 diabetes in the early stage. In experimental animals, there was a violation of glucose tolerance and a decrease in the sensitivity of peripheral tissues to the action of insulin.

At the end of the experiment, the rats were decapitated under thiopentane anesthesia.

Table 4

The effect of the declared herbal antidiabetic compound Me 4 on the development of tolerance to carbohydrates in animals with type 2 diabetes induced by the administration of dexamethasone, (teape5E, n-8)

Term. . . Pathology of observation, Intact Control Pathology of shi Pathology t pill collection min group pathology antidiabetic Arfazetin", 9 metformin, 60 collection Mo 4, 12 ml/kg ml/kg'

MG/KG glycemia

Table 4 (continued)

Term. . . Pathology of observation, Intact Control Pathology M - Pathology t pill collection min group pathology antidiabetic | "Arfazetin", 9 metformin, 60 collection Mo 4, 12 ml/kg ml/kg mg/kg ' 60 | 40450.07 | 12.56:0.317 | 742-026 | 848:0.527 | 6460"

Notes: 4. - the differences are statistically significant in relation to the values of the intact group, p<0.05 (according to O-criteria

Mann-Whitney); 5.7 - the differences are statistically significant in relation to the values of the control pathology, p«e0.05 (according to 0О- Mann-Whitney criteria); b. n - the number of animals in each group.

The results of the study showed that the declared plant antidiabetic collection Mo 4 in a dose of 12 ml/kg exhibits pronounced antihyperglycemic activity in animals with experimental diabetes, which was manifested by a reliable decrease in basal glycemia by 31 95 relative to the control pathology and contributes to the reduction of glucose tolerance disorders, which was manifested reliable reduction of blood glucose for 60 minutes of the intraperitoneal tolerance test almost to the initial value. Therefore, the declared plant collection Mo 4 shows antidiabetic activity, surpassing the comparison drug collection "Arfazetin" according to the pathogenetic mechanism of action: it reduces insulin resistance and normalizes impaired glucose tolerance.

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K.: Morion. - 2013. - 2270 p. 2. RuggupezKa K., ZepikomeKa A. Spgotaiyodgarnis Apaiuziz oi Roiurpepoiv / K. Ruggup5Ka, A. zZepikomuzKa //RoiYurnepo!v ip Riapiv. - Asadetis Rgezv. - 2019. - b. 353-364.

Z. Sotrbipipa rgezzigigea Igdiydv m/p yigavoshpa o itrgome ilye ehigasiyup oi rpepoiis sotroypav iot roteagapaie rev! (Rypisa dgapaisht 1.) / V. A. bBitege eyi ai. //Ngavopisv5 zopospetiviga. - 2018.-T. 48. - b. 151-162. 4. Methods of biochemical research (lipid and non-energetic exchange). Textbook/ Ed. WE. Prokhorova. - L.: Izd-vo Leningr. unta - 1982.-272 p. 5. Directive of the Council of the EU on approximation of laws, decrees and administration of the state of the EU on the protection of animals used for experimental and other scientific purposes (86/609/EEC) / Ed. ON. Lyapunova, V.A.

Zahoria, V.P. Georgievsky, E.P. Bezugloi // In the book: Appropriate production practice of medicinal products. - Kyiv. "Morion". - 1999. - P. 508-545. 6. Preclinical research of medicinal products: Method, recommendations / Ed. member-cor. AMN

of Ukraine O.V. Stefanova. - K.: Avicena, 2001 - 528 p. 7. GuzinKk V. The use of medicinal plants in diabetes (ip OKhaipiap) - Arriisayop

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Claims (1)

USEFUL MODEL FORMULA A collection of medicinal plants for the prevention and treatment of type 2 diabetes, which includes 35 rosehip fruits, which is distinguished by the fact that it additionally contains burdock roots, wheatgrass rhizomes, corn cobs and cumin flowers, and all ingredients are taken in the following ratio, by weight. h.: burdock roots 5 wheatgrass rhizomes 5 corn stalks with receptacles 4 cumin flowers Rosehip fruits 2.