UA142461U - COMPOSITION FOR INCREASING EGGS derivability chickens, O young and pollution prevention hatching eggs pathogenic UNDER poultry farms countries most affected by global warming - Google Patents

Abstract

The composition for increasing hatchability of hens' eggs, hatching of young and prevention of contamination of hatching eggs with pathogenic microflora, in the conditions of poultry farms of the countries most exposed to global warming contains non-toxic, widespread and inexpensive substance of natural origin - acid-soluble% chitosan 2.5-3.0), which has powerful biocidal properties against pathogenic microflora of bacterial, viral and fungal nature. The composition contains additional components: hydrogen peroxide (H2O2), nanosized calcium carbonate (CaCO3), water softener, trace elements (magnesium, cobalt, zinc, copper) and water.

Description

The useful model belongs to the field of agriculture, namely industrial poultry farming, and can be used for processing hatching eggs of chickens in the conditions of poultry farms of countries most exposed to the influence of global warming in a complex of measures to protect against pathogenic microflora of bacterial and viral origin during incubation by applying to the surface hatching eggs of the solution, which includes a substance of natural origin - chitosan in combination with powerful disinfectants from the group of organic peroxide compounds, peracetic acid (NOK) and hydrogen peroxide, as well as ultra-nanosized particles of calcium carbonate (CaCO3) with the subsequent formation on the surface of the eggs of a biocidal , water-retaining and gas-permeable and at the same time ecologically safe protective film.

It is known that despite the rapid growth and continuous improvement of the overall production indicators in the egg and meat poultry sectors in the world, there are a number of limitations affecting the future growth of this industry, among which environmental problems related to global warming (Raptezap, S., Mops, S. A diobaynu soPegepi Pipdegrgipi oi sitaiye spapde itrasiv asgo55 paishgai! zuzietv. Maishge. - 2003. -421. -37-42), especially in countries with a hot climate, which causes severe stress birds and leads to a decrease in productivity IRgadua Vnadatsytia, U.M. Kaiagia, 5. Ma)itaag sei ai. Itrasi oi Moi Siitaie op Roskhgu Rhodisiyup Zubvivt-A Nemivm //Soshttai oi Roshigu Zsivepse apa Tesppoiodu. - 2014.- M. 2, (4). -R. 56-63). Taking this into account, reviewing the place of influence of heat stress on the physiology of birds in hot climate conditions and its detrimental effect on poultry farming, in particular in incubation, is currently a priority area of research. E., 4

VesKtapp, S. Nidp aitozrnNetis ietregaigez apa "atbriepi ipsibayop" apyme etbguopis aemeortepi apa Ieasd (o vapieg Naisnipad ip a razzeiipe bBiga. //Yaouai! osieiu Orep Zsiepse, - 2016. - Z(2). -150371;

Oypp RO, M/ipKiety YuMU. 2010 EpNesiv oi siitaiye spapde op iyetipa oi Bheedipd apa gergodisiime vyssevz5 ip Bigav5. Ip EMNesiv oi sitaie spapde op bBiga5 (vd5 A MaiIeg, UM Rieaeg, R Veppoia). - R. 113-128. Ohgoga, OK: Ohtoga Opimegeyu Rgez5 Mer OV. 1987; Tpegtai yuiegapse oi amiaap etrguov: a hemiem/. Sopaog. - 89. -874-898; Agai 7, Samtgivii-I emip I, Magayeg U. 1988 Adariayop oi ilye rideop eda tyu ipsibayop ip agu poi epmigoptepiv. РНузиой 2001. - 61. - 293-300; 5tii V, MeKesppiv AE. 2010

Amiap Zeazopa! teiaboiis magiayop ip a 5irigorisa! dezep: times! teiarois haiev age Iomeg ip m/pieg

Shap ip witteg. Ripsey Essays - 24. - 330-339; Agaia OV, Sooreg SV, Opopai AA. 2006 Uagt Tetregaishtev5 Ieai (0 eapu op5ei oi ipsibayop, 5popeg ipsibayop regiod5 apa adgeaieg Naispipd azupspgopu ip igeye zulaiyom5 Taspusipeia Bisoiog ai Te ehigetez oi INEig hapde. U. Amiap Vio!. - 37. - 137-142).

A well-known substance for the protection of hatching eggs of farm poultry is formaldehyde in the form of steam | (Disinfection of hatching eggs in industrial incubation. Methodical recommendations. - M.: Moskovskaya veterinarnaya akademiya, 1983. - 34 p.|.

Known compositions for the protection of hatching eggs of poultry based on chitosan and peroxide compounds (Bordunova O.G., Astrakhantseva O.G., Baidevlyatova O.M.,

Chivanov V.D. Stalemate. 59917 Ukraine, IPC AO1K 43/00, AO1K 41/00. The method of protection of hatching eggs of chickens with chitosan coating /- Mo c201011919; statement 08.10.2010; published 10.06.2011, Bull. Mo 11.

Bordunova O.G., Astrakhantseva O.G., Baydevlyatova O.M., Chivanov V.D. Composition for protection of hatching eggs of chickens /61. Stalemate. 72945 Ukraine, IPC AYETI. 2/18 (2006.01). MO and 201112186; statement 18.10.2011, publ. 10.09.2012, Bull. Mo 17; Bordunova O.H. Composition for disinfection of hatching chicken eggs. Stalemate. 93549 Ukraine, IPC AO1TK 43/00 (2014.01). - MO and 201403517; statement 04/07/2014, publ. 10.10.2014, Bull. Mo 19; Bordunova O.G.; Composition for protection of hatching chicken eggs against pathogenic microflora. Stalemate. 93550 Ukraine, IPC AOTK 43/00 (2014.01). - MO and 201403518; statement 04/07/2014, publ. 10.10.2014, Bull. Mo 19; Bordunova O.H. A composition for the destruction of pathogenic microflora on the surface of hatching chicken eggs. /Stalemate. 93551 Ukraine, IPC dO Kk 43/00 (2014.01). - MO and 201403519; statement 04/07/2014, publ. 10.10.2014, Bull. Mo 191.

A known substance from the class of quaternary ammonium compounds is trimethyloctadecyl ammonium bromide in a mixture with perhydrite (the drug Perbaksan) (VO2392005 IPC AETI 2/18. 20061.

Known compositions for disinfection based on peracetic acid, as an inexpensive, low-toxicity, environmentally safe, powerful biocidal substance, effective in a wide range of temperatures, even against spore forms of microorganisms (RZ-oxoniyF active (EKOLAB), OIMO5AM ACTIM, Neoseptal - RE (Dr. Weigert ), KRIODEZ, AQUADEZ-NUK G|Nettipd

N.-S. Oie Regezzidzatsyge az OeezipieKkiopvzticei!. Eip Oretbiisk //2riI. Wack!. Boredom. - 1984. - Va. 179, Mo. 2. - 5. 97-111; Vgipe M/.3., Void-Zadiez A.H., Negpapae? Ne!tego M.M., I ore2-Redetopia T., Scativ

V. Vasiegisida! eiyysasu oi regaseiis asii ip sotbripayop myy puagodep regohide adainvi raiitpodepis apa pop raiiodepis vigaipv oi tarpuiosossis zrr., I iziepa 5yr. apa EzsNegisnia soii //Rosa Sopikgoi. - 2006. - M. 17. - R. 516-521; Zaa Irizdiiga R., Neitega -.9.V., Saro M.I. Veziziapse o repgaIKopiit sPiogide, regaseiis asii apa pibvip digipud Toptaiyiop oi taishte rioiitv5 Bu I ibieegta toposuiidepe5

Rosa Mystoriiodu. - 2011. - M. 28. - R. - 418-425).

Known ecologically safe and highly effective biocidal compositions based on hydrogen peroxide (H2Og) and peracetic acid | ip sotragibzop m/yp spiogipe apa

ToppaIdenuae adaipwi Basiegia! mayeg vigaip5. //Sapadiap mahdtai oi Misgobioiodu. - 1992. - M. 38, Mo. 7. - R. 635-642).

A well-known composition for the destruction of organic pollution, including pathogenic microflora, based on a variant of the effective oxidation process (admapsei ohidayop rgosev55ev5

AOR), which is based on the combination of hydrogen peroxide NgO»g and tri- or divalent iron ions Re (IP), Re (II) |(Tegsapi-Stsueg Si., ipse M.N. /Opgazop. Zopospet. - 2003. - M. 10. - R. 235-240).

The specified substances and compositions have certain disadvantages: a) formaldehyde vapor is a potential carcinogen and irritant of the respiratory tracts of poultry workers; in addition, the duration of the biocidal effect of formaldehyde is quite limited, taking into account the increased volatility of the substance; b) preparations for the protection of hatching eggs, which include quaternary ammonium compounds, are not environmentally safe due to their high resistance to the destructive action of beneficial microflora of the environment; c) the wide use of quaternary ammonium compounds in veterinary and humane medicine has led to the acquisition of significant resistance of certain representatives of pathogenic microflora to the latter; d) films of quaternary ammonium compounds on solid-phase surfaces are not sufficiently gas-permeable, due to which protective preparations for hatching eggs based on them require an increased level of compliance with processing technologies; e) peracetic acid is quite volatile and quickly destroyed, which does not exclude secondary contamination of treated surfaces; e) peracetic acid belongs to the group of corrosive-active substances; g) chitosan has moderate biocidal properties within certain limits of the acidity of the environment; k) ultra-nanodisperse particles of metal oxides do not sufficiently cover the solid-phase surface on which they are applied, in addition, the layers of these oxides are easily destroyed, in particular under the influence of mechanical factors.

The basis of a useful model is the task of developing a composition for processing

From the hatching eggs of chickens in the conditions of poultry farms of the countries most exposed to the influence of global warming in the complex of measures to protect against pathogenic microflora of bacterial and viral origin during incubation, which would be expected to form on the surface of the eggs similar to the natural natural protective coating of bird eggs - the cuticle, protective elastic selective gas-permeable water-retaining composite film with a thickness of 0.05-10.0 μm made of environmentally safe non-toxic organic and inorganic substances, in particular of natural origin, which had 6 inherent powerful biocidal properties against pathogenic microflora.

The task is solved by the fact that the composition for increasing the hatchability of chicken eggs, the hatching of young and preventing the contamination of hatching eggs with pathogenic microflora, in the conditions of poultry farms of countries most exposed to the influence of global warming, containing a non-toxic, widely distributed and inexpensive substance of natural origin - acid-soluble chitosan (pH 1 95 solution in 2 95 peracetic acid 2.5-3.0), which has powerful biocidal properties against pathogenic microflora of bacterial, viral and fungal nature, according to a useful model, contains additional components: hydrogen peroxide (HgOg), nanoscale calcium carbonate (СаСОз) water softener, microelements (magnesium, cobalt, zinc, copper) and water, in the following ratio of components (by mass): acid-soluble chitosan (pH 1 95 of a solution in 2 95 peracetic acid 2.5-3.0 ; sorption activity for copper ions 80.3 mg/g) 0.1-3.4 hydrogen peroxide (HgOg) 0.5-5.5 calcium carbonate (CaSoz) nanoscale 01-22 water softener and 0.05-0.1 trace elements (magnesium, cobalt, zinc, copper) 0.01-0.1 water to 100.

Chitosan is a derivative of chitin, which is extremely widespread in nature, non-toxic, ecologically safe and cheap material, which is extracted in large industrial volumes from crustaceans and insects and which has powerful biocidal properties against pathogenic microflora. Maishga! Speiaipd Roiutetgv: AIIDipis asia, Spiip apa Spiyuzap /Ohio: Regdatop Rgezv5, 1973. - 574 r.; S.A. Vobegiv... Spetsip spetivigu /Noipatiyv: Mastiyap, 1992. - 486; Kitag! M.M.M.V. A hemivm/ oi spyip apa spiuozap arriisayop5 //Veasey. Biopsies. Roiut. - 2000. - M. 46. - R. 1-27; Vipatsdo, M. Snip apa spyozap: rgorepie5 apa arriisayopv. Year RoiYut. axis - 2006. - 31. - 603-32.; RiPai, S.K.5, Rashtsi, MU., Zpagpta, S.R. Spyp apa spiyuzap roiYutegv: spetivigu, zoiShriyu apa tag Toptaiyop. Year Roiut. 5 - 2009. - 34. - 641-781.

The theoretical basis for the construction of the composition was, firstly, the modern direction in disinfection, in particular, the combination of different active substances in one preparation with the aim of enhancing the beneficial properties (biocidal activity) and inhibiting the undesirable ones (corrosive activity) in a synergistic relationship

Rgasiise oi Oiviptesiop /Ea. Wu A.R. Rgaize, R.A. And atbeni, 9U.-M. Mayaga. - 2004. - OK: Viaskmeiyi

Zsiepse Ia. - 688) and, secondly, the prospect of using a biomimetic approach in industrial biotechnologies. In our case, the improvement of hatchability indicators of hens exposed to elevated temperatures during storage, which is an important risk factor in the conditions of poultry farms in countries suffering from global warming, is ensured by the introduction into the composition of the composition together with the hydrophilic chitosan matrix of nanosized particles of calcium carbonate, which also has powerful adsorption effect on water vapor, as well as other organic and inorganic compounds (pdgid Sogalagi, Vobepo Miwiiso,

Egapsezso Tigsi, Magia Sischia Rada, Riamia Egap2o5o, 5imia Taraz550o, Sishiapa Madpassa Admapsei rpuziso-spetisa! sNpagasiegigayop oi spyozap Bu teap5 oi TA soiciried op-ype m/y ETIAV apa

CSM5: Tnpeptai! ayedgadayop apa ulaieg azogrip sarasiu. Roiuteg YuOedgadayop apa etariiyu. - 2015. - M. 112-1-9. Vipatsdo, M. Spyip apa spyozap: rgorepiez apa arriisayopv5. Year Roiut. b. - 2006. - 31. - 603-321). The need for hydrophilic, water-retaining qualities in the artificial cuticle formed during the drying of the working solution on the surface of the hatching bird egg is due to the fact that the natural cuticle is initially hydrated and structured in such a way as to ensure transshell moisture supply (at the same time, the penetration of bacteria is also possible). In the next period, the natural cuticle becomes dry and prevents the further movement of moisture. The cuticle undergoes even greater changes during the storage of hatching eggs under the conditions

From elevated temperatures: increasing the duration of storage of hatching eggs per day leads to an increase in the legality of embryos by approximately 195 and requires a 40 min extension of the incubation period. In general, the quality of chicks deteriorates after long-term storage of eggs. For long-term storage of hatching chicken eggs for more than 7 days, the optimal temperature is 12-13 "C and humidity index 75-80 95 | Verbytskyi, S. Pre-incubation storage of eggs. Our Poultry, 2014. - Mo 5. - P. 31-33; Shomina N., Tkachenko S., Baydevlyatova 0.,

Ogurtsova N. The optimal mode of incubation of eggs after long-term storage. - Animal husbandry

of Ukraine. - 2011. - 21, 1-2. - 18-211.

The proposed composition of the composition for the prevention of contamination of hatching chicken eggs with pathogenic microflora contains the following components, wt. 90: acid-soluble chitosan (pH 1 95 of a solution in 2 95 peracetic acid 2.5-3.0; sorption activity for copper ions 80.3 mg/g) 0.1-3.4 hydrogen peroxide (HgOg) 0.5- 5.5 calcium carbonate (CaSoz) nanosized 01-22 water softener 0.05-0.1 trace elements (magnesium, cobalt, zinc, copper) 0.01-0.1 water to 100.

The given composition of the composition for the prevention of contamination of hatching eggs of chickens with pathogenic microflora contributes to an increase in the indicator of hatchability of eggs by 25.8-21.5 95 (Table 1) and a decrease in the number of pathogenic microflora on the surface of eggs by 97.7-99.01 9o from the initial amount bacterial colonies (MPA medium) (Table 2).

The above is confirmed by the following examples:

Example 1.

A film is formed on the surface of edible eggs by spraying with an aqueous solution of chitosan (1 95, pH 5.0), which is immediately subjected to radiation treatment (2.0 kGy) in order to improve and preserve the quality of eggs during long-term storage (Hiap Oye Yei Yei Ani. EmNesi oi sotbripaiop oi spiuz5ap soaiipdu apa itaadiainop op rpuzisospetisa! apa gypsiyopa! rgorepievv oi listkep eda digipa goot-Iietregaiishge viogade //Nadiainop Rpuzisv apa Spetivigu. - Moi. 78, Ivvtsev 7-8. - 2009. - R. 589- 5911) .

Similarly to example 1. The difference is that, firstly, in the indicated analogue, chitosan is dissolved in food acetic acid, secondly, in the composition of the solution for the formation of a protective coating on hatching eggs, in addition to chitosan in concentrations of 0.1-3.4 (depending on the original quality of the eggs), NOK and water include additional substances: nanosized calcium carbonate (CaSoOz), hydrogen peroxide (HgOg), water softener and trace elements (magnesium, cobalt, zinc, copper). The acidity of the solution (pH) does not exceed 3.0. Nanosized calcium carbonate was prepared according to the method: Zagapeipi, A. Aiaii, 5.M. zZaiy, A.A. Nozviipiop Ope-5ier Gasiie 5upipeziz oi SaSOz paporapisiyez mia tespapo-spetisa! Mr. Romdeg Thesppoiodu. - 2012. - 219. - 72-17 with modifications. Nanoparticles of calcium carbonate from the precursors CaSi" and MagzhbOz were synthesized by mechanical and chemical processing without further thermal impact on the ground powder. The powder mixture was milled once in a planetary ball mill with Masi as a thinning agent and a second time with KSI. The level of nanoscale dispersity of calcium carbonate CaCO3 was achieved after 10 min of continuous grinding in a planetary ball mill. The size of the particles is from 60 to 100 nm.

Example 2.

To improve the quality of edible eggs and improve the quality of the shell, the authors used a method of covering the latter with a film containing chitosan and lactic acid

ISepgid Sapeg, O27de Sapvi?7 Spiyuzap soaiyypd tipitisez eddeNei! ogeaKkade apa itrgomez edd diaiyu pdoitai oi She Zsiepse oi Rosa apa Adgisinige. - 2007. - Mine. 88, Iv5ce 1. - R. 56-611.

Similarly to example 2. The difference from the analogue is in the composition of the protective film - in our case, the use of lactic acid is impractical, since this substance will impair the gas permeability of eggs.

Example 3.

On the surface of hatching eggs, by spraying with a 0.1-3.0 95 solution of chitosan in 2 95 peracetic acid (pH 3.6), a gas-permeable film with pronounced biocidal properties is formed, which protects against negative environmental factors and pathogenic microflora | patent on

From the useful model Mo 59917 "Method of protection of hatching chicken eggs with chitosan coating", Authors:

Bordunova O.G., Astrakhaptseva O.G., Baydevlyatova O.M., Chivanov V.D.| Similarly to example 3.

The difference from the analogue is that, firstly, in our case, chitosan is dissolved in 2 95 peracetic acid (NOK), secondly, in the composition of the solution for the formation of a protective coating on hatching eggs, in addition to chitosan in concentrations of 0.1-3 ,4 (depending on the original quality of the eggs), NOK and water include additional substances: hydrogen peroxide (HgOg), nanosized calcium carbonate (CaSo»z), water softener and trace elements (magnesium, cobalt, zinc, copper).

The acidity of the solution (pH) does not exceed 2.5-3.0.

Example 4.

In the experiment, eggs of two breeds of chickens were incubated - Poltava Clay (2800) and Birkiv Varvysta (2800), which were stored for 10 days at a temperature of 15 "С, humidity - 70 95.

Incubation was carried out according to the established methodology (Incubation: Method, manual / V.O. Breslavets,

E. Sakhatskyi, B.T. Hip and others. - IP of the Ukrainian Academy of Sciences. - Kharkiv, 2001. - P. 56). Incubator "Universal 55"; the age of the bird is 40 weeks.

An analogue is a variant of the experiment, where the classical method was used - treatment with formaldehyde steam.

Table 1

Results of incubation of eggs of chickens treated with the proposed composition before incubation

Laid, IUnfertilized eggs, H cancer of incubation, Hatching Hatchability

Couple

Couple

Presented in the table. 1, the data show that the indicator of hatchability of eggs is 85.1-84.5 95, which is significantly higher than in the variants where processing by the classical method was used - formaldehyde vapor 59.3 and 63.0, respectively.

Example 5.

Research on the biocidal activity of the composition for the destruction of pathogenic microflora on the surface of hatching chicken eggs was carried out by microbiological methods, according to DSTU 4769:2007.

An analogue is a variant of the experiment, where the classical method was used - processing with formaldehyde steam.

Table 2

Microbial contamination of hatching chicken eggs during incubation, (MPA, KUS), XX

After treating the vices

After processing

Note: "p" 0.05.

The useful model makes it possible to increase the rate of hatchability of eggs from hatching eggs that were stored under conditions of elevated temperatures by 25.8-21.5 95, as well as to reduce the number of pathogenic microflora on the surface of eggs during incubation by 97.7-99.01 95 from the control.

Claims (1)

USEFUL MODEL FORMULA A composition for increasing the hatchability of chicken eggs, the hatching of young and preventing the contamination of hatching eggs with pathogenic microflora, in the conditions of poultry farms in countries, 20 most affected by global warming, containing a non-toxic, widespread and inexpensive substance of natural origin - acid-soluble chitosan (pH 195 of a solution in 2 95 peracetic acid 2.5-3.0), which has powerful biocidal properties against pathogenic bacterial, viral microflora and fungal nature, which differs in that the composition contains additional components: hydrogen peroxide (HgOg), nanosized 25 calcium carbonate (CaSoOz), water softener, trace elements (magnesium, cobalt, zinc, copper) and water in the following ratio of components (wt. b): acid-soluble chitosan (pH 1 95 of a solution in 2 95 peracetic acid 2.5- 3.0; sorption activity for copper ions 80.3 mg/g) 0.1-3.4 hydrogen peroxide (HgOg) 0.5-5.5 calcium carbonate (CaSoz) nanosized 01-22 water softener 0, 05-0.1 trace elements (magnesium, cobalt, zinc, copper) 0.01-0.1 water to 100.