UA136662U - METHOD OF MEDICAL CORRECTION OF EXPERIMENTAL DIABETES MELLITUS IN RATS - Google Patents

Abstract

A method of drug correction of experimental diabetes mellitus in rats by prescribing a drug with hypoglycemic action. Once orally administered dry extract of the roots and rhizomes of ergot high at a dose of 0.11 g / kg.

Description

A useful model belongs to the field of medicine, namely pharmacology, and can be used to correct type II diabetes in rats.

Diabetes mellitus (DM) belongs to a pathology that has a significant share in the development of incapacity, disability and mortality of the population (V. I. Pankiv, 2013; L. G. Polozova, 2013). Today, there are about 382 million people with diabetes in the world, their number continues to grow, according to the International Diabetes Federation, this number will increase by 55,95 (V.V. Poltorak, 2015).

The number of patients with diabetes is increasing mainly due to the development of type II diabetes (O.V. Tkachenko, 2014).

The rapid increase in the incidence of diabetes necessitates the search for and creation of new effective drugs. For patients with type II (non-insulin-dependent) diabetes mellitus, oral hypoglycemic drugs, which are mostly synthetic in nature, are the first priority.

The treatment program for diabetes is approved by the clinical protocol for the provision of medical care (Order of the Ministry of Health Me1118 dated 12.21.2012).

The closest analogue of a useful model is the method of correction of experimental type I diabetes (Nedosugova L.V. Glybenclamide - profile of effectiveness and safety.

Diabetes mellitus. 2011, Meo3. P. 85-90), in which a drug from the group of sulfonamides (derivatives of sulfonylureas) - glibenclamide is prescribed; if the main normalization of the blood sugar level, both fasting and after eating, is not achieved, insulin therapy or monoinsulin therapy is additionally prescribed.

Disadvantages of the analogue method are the often low effectiveness of treatment due to side effects and the limitation of long-term use. Relative contraindications to sulfonamide therapy are minor surgical interventions, cardiovascular disasters (myocardial infarction, stroke), diabetic angiopathy (retinopathy, diabetic angiopathy of the vessels of the lower extremities). In some patients, sulfonamides can cause side effects such as dyspepsia, allergic reactions (from skin to general manifestations), changes in the blood (leukopenia, thrombocytopenia, agranulocytosis), liver damage (cholestatic hepatitis against the background of taking chlorpropamide). Some patients develop primary (after taking the drugs) sulfonamide resistance. Secondary occurs much more often

With sulfonylamido resistance. In the case of long-term use of drugs, the development of resistance to them, a noticeable deterioration of lipid metabolism, acceleration of the development of atherosclerosis of large and small vessels, the formation of the so-called diabetic retinopathy, nephropathy, and angiopathy of the extremities have also been noted.

The basis of the useful model is the task of improving the method of drug correction of experimental diabetes by means of a single oral administration of a dry extract of the roots and rhizomes of the high oleander.

Common features of the useful model and the closest analogue are the appointment of a hypoglycemic drug.

Distinctive features of the useful model from the closest analogue are that the dry extract of the roots and rhizomes of the high delusion is administered orally once at a dose of 0.11 g/kg.

Definition of terms used in the description of the useful model: drug correction, experimental diabetes, dry extract of the roots and rhizomes of the high delusion, hypoglycemic effect.

Theoretical prerequisites for the emergence of a useful model.

Given that the incidence of diabetes is high both in Ukraine and in global practice, the search for new drugs is urgent. Today, the pharmaceutical market is represented by a wide arsenal of synthetic drugs used for the prevention and treatment of patients with diabetes. For example, glibenclamide, which is used in the method, is the closest analogue. Glibenclamide has a hypoglycemic effect both in patients with diabetes

Type II, as well as in healthy people, because it increases the secretion of insulin by B-cells of the pancreas due to their stimulation. This action depends on the concentration of glucose in the environment surrounding the B-cells. On the other hand, the use of glibenclamide has a side effect, due to which the duration of its use is limited.

An alternative to these synthetic agents are plants, which can serve as a potential source of hypoglycemic agents and are widely used in traditional medicine to prevent diabetes. The action of natural compounds is more physiological for the body than their synthetic counterparts, as they contain a complex of biologically active substances that affect several body systems simultaneously, which provides a positive clinical effect. One of the promising medicinal plants that can serve as a raw material for the production of new drugs is the oleander (Ipsha Peiepisht Y.). The effect of high delusion as an anti-inflammatory, expectorant and antimicrobial has been proven.

The use of the extract of oleander at the expense of a complex of biologically active substances (insulin-like substances (pseudoinulin, inulinin), gums, saponins, organic acids, vitamins, etc.) shows a hypoglycemic effect in type II diabetes, in addition, it has a normalizing effect on vegetative and cardiovascular vascular system, liver function, affects glucose absorption processes in the intestines, increases tolerance to carbohydrates.

The use of the extract of the high delusion in mild forms of diabetes will make it possible to do without drugs, and in some cases to reduce the daily doses of oral antidiabetic drugs and stabilize the course of the disease.

A study was conducted. 30 sexually mature white males weighing 180-220 g, which were kept in vivarium conditions, were modeled with type 11 diabetes with the help of intraperitoneal administration of glucose at a dose of 3 g/kg, which leads to the development of acute hyperglycemia. Thus, the level of glucose in the blood of animals that received a glucose load exceeded 2.17 times compared to the control.

The animals were divided into 2 groups of 15 rats each. For the correction of experimental type II diabetes in the first group, a dry extract of the roots and rhizomes of the high oleander was used in a dose of 0.11 g/kg with a single injection, and the second group of animals remained untreated. Under the influence of a one-time administration of the extract of the high delusion in a dose of 0.11 g/kg, the level of glucose in the blood was reduced by 1.85 times in comparison with the control pathology. At the same time, the hypoglycemic effect for 15 minutes of the experiment was 46.08 Vol.

A useful model is implemented as follows.

Animals simulate type II diabetes, for which glucose is injected intraperitoneally in a dose of 3 g/kg, which leads to the development of acute hyperglycemia. To correct the experimental

DM of its type is prescribed once orally dry extract of the roots and rhizomes of high delusion in a dose of 0.11 g/kg.

Examples of the use of a useful model.

Animals were modeled with type II diabetes, for which glucose was injected intraperitoneally at a dose of 3 g/kg, which led to the development of acute hyperglycemia, with a sugar level of 10.2

From mmol/l For the correction of experimental type II diabetes, a dry extract of the roots and rhizomes of the high oleander in a dose of 0.11 g/kg was administered orally once. After 15 minutes, the sugar level decreased to 5.5 mmol/L and was close to the initial level, which was 4.0 mmol/L. Therefore, the introduction of a dry extract of the roots and rhizomes of the high delusion leads to an effective decrease in the level of glucose in the blood.

Technical result. The proposed method allows for effective drug correction of experimental type 2 diabetes in rats using a dry extract of the roots and rhizomes of oleander high, which does not show side effects, is low-toxic, can be used for a long time as a monopreparation, or in combination with synthetic drugs.

Claims (1)

FORMULA OF A USEFUL MODEL Method of drug correction of experimental diabetes in rats by prescribing a drug with hypoglycemic action, which is characterized by the fact that a single 45 orally administered a dry extract of the roots and rhizomes of the high delusion in a dose of 0.11 g/kg.