UA104161U - Herbal species for treatment of diabetes mellitus type ii - Google Patents

Abstract

Zbir likarskih roslins for liking the disease for type II diabetes mellitus including horsetail polish grass, wild grass, blackberry leaves and spring leaves to retrieve elderberry leaves, omanu root crops and root leaves, argue.

Description

The useful model belongs to pharmacy and medicine, namely medicinal herbal preparations, and can be used for the treatment of diabetes patients, both independently and as part of complex therapy.

Diabetes mellitus is a global medical and social problem, which is due to the epidemiological nature of the spread of this disease and the development of micro- and macroangiopathies, which significantly reduce the quality and life expectancy of patients.

The main pathogenetic mechanisms of type II diabetes are insulin resistance, dysfunction of B-cells of the pancreas and excessive production of glucose by the liver. Pharmacotherapy of type 1 diabetes should be aimed at preserving (or improving) the function of B-cells of the pancreas, increasing the sensitivity of peripheral tissues to the action of insulin, stabilizing the level of glycemia and restoring metabolism in general. For this, it is necessary to use drugs with multidirectional action or combined therapy aimed at different links of the pathogenesis of type II diabetes.

In this aspect, it is promising to create remedies based on medicinal plants, the use of which in the treatment of diabetes has centuries-old empirical experience of folk medicine and at the current stage is supplemented by new scientific data on the mechanisms of their action.

A well-known collection of medicinal plants "Arfazetin" for the treatment of type II diabetes, which contains blueberry common shoot, bean common fruit leaf, Eleutherococcus spiny rhizome and roots, rose hips fruits, horsetail of field grass, St. John's wort, chamomile flowers 1. This herbal remedy is used for treatment patients with diabetes in the form of an infusion, which is prescribed orally at 100 ml 2 times a day for 30 minutes. before eating

The disadvantage of the known collection is insufficient pharmacological activity, resulting from the limited content of pharmacologically active compounds of plant components with antioxidant, hepatoprotective, cardioprotective effects and its undesirable use, in particular during pregnancy, in hypertension and in pediatric practice, due to the presence of rhizomes and roots of Eleutherococcus .

The basis of the useful model is the task of improving the well-known collection, in which by changing the composition of its ingredients aimed at increasing the content of biologically active substances with anti-inflammatory, antioxidant, hepatoprotective, cardioprotective,

With a gastroprotective effect, which, in turn, leads to an increase in the detoxification capacity of the body in the treatment of patients with type II diabetes, and therefore to an increase in the pharmacological activity of the collection as a whole. The composition of the claimed collection includes the rhizome and roots of the delusion, which are rich in inulin, which contributes to the reduction of glucose in the blood and the normalization of fat metabolism; nettle leaves, which contain a significant amount of ascorbic acid, which has an antioxidant effect; carotenoids, which have a positive effect on the function of the organs of vision; vitamin K, which has a hemostatic effect. Linden flowers contain a lot of vitamin C, carotenoids, flavonoids, which have general strengthening and adaptogenic effects.

When solving the technical problem, it was also taken into account that pharmacologically active compounds with anti-inflammatory, antioxidant, hepatoprotective, carbioprotective, and gastroprotective effects are also found in large quantities in elder flowers, sedge grass, and St. John's wort.

Based on the above, the task is solved by the fact that the collection of medicinal plants for the treatment of patients with type II diabetes, which includes horsetail grass, St. John's wort, blueberry leaves, according to a useful model, additionally contains elderflower, rhizomes and roots , linden flowers, sedge grass, nettle leaves, and all ingredients are taken in the following ratio, mass h.: horsetail field grass 2.0 St. John's wort grass 1.0 blueberry leaves 3.0 elder flowers 1.0 rhizomes and roots delusion 1.0 linden flowers 1.0 spurge grass 1.0 nettle leaves 1.0.

The declared fee is prepared as follows.

Collected and dried in accordance with the regulatory rules of procurement, medicinal plant raw materials are weighed in the following amount, based on 100 g of the finished product, namely: horsetail grass 18.2 St. John's wort grass 9.1 blueberries leaves 27.3 elder flowers 9.1 rhizomes and roots 9.1 linden flowers 9.1 sedum grass 9.1 nettle leaves 91.

Grind suspended materials to the appropriate particle size: flowers, grass, leaves - up to mm; rhizome with roots - up to 3 mm. The crushed ingredients are sieved through a sieve with a hole size of 0.2 mm. The mixing of plant components is performed on a sheet of paper, in a wide mortar or porcelain cup using a spatula or spatula. First, plant 5 materials prescribed in smaller quantities are mixed, then gradually added in larger quantities.

In order to treat patients with type II diabetes, the collection is used in the form of a decoction at a ratio of 1:10. To do this, take 20 g of the collection, fill it with 240 ml of water (taking into account the water absorption coefficient Kv - 2), heat it in a boiling water bath for 30 min., then cool it at room temperature for 10 min., filter and squeeze the raw materials, add water, proving decoction up to 200 ml. The indicated phytoremedial agent is taken internally in 100 ml 2 times a day for 30 minutes. before eating

A useful model is illustrated by examples.

Example 1. The quantitative content and qualitative composition of the declared fee for the content of biologically active substances that cause pharmacological activity were studied. The results are shown in Tables 1 and 2.

Table 1

Quantitative content of biologically active substances in water and water-alcohol extracts of the antidiabetic collection

Ascorbic balls Organic. Hydroxycinnamic

BAR R DU r Flavonoids dr r acid substances acids acids

Note: BAR is a biologically active substance

Table 2

The content of macro- and microelements

Elements | Ma| K | Sa | Mo | hey | Sy |l/l|Mp| mi | sa | R | 5e | si

Content, mg/kg 7255 | 8910 | 3714 0.031 | 2275

Note: n/a - not detected

Example 2. Determination of the hypoglycemic effect of the collection on rats with normal glucose homeostasis.

To determine the hypoglycemic effect, the plant collection was administered in doses of 3, 6, and 9 mg/kg to normoglycemic female rats weighing 250-300 g intragastrically for 3 days.

Control animals received water. The level of glucose in blood samples obtained from the tail vein of animals was determined dynamically: initial data, after 1, 2 and 3 hours after the introduction of collection by the glucose oxidase method using test kits "cSlyukose-D" of the company "Filisit-Diagnostika" (Ukraine ).

The obtained data are presented in Table 3.

Table C

Dynamics of the hypoglycemic action of the antidiabetic collection during intragastric administration to normoglycemic rats (Mat) value (Negative control | - | 32501 | 36:02 | 3750.03 | 34016

Plant collection 60 | 304502 / 34007 | for | 33:02

And .90 | 383-404 | 331 | 34009 | 3265016

Note. n-5 - the number of animals in each group.

The study showed the absence of a hypoglycemic effect of the collection, since the glucose level under the influence of the agent did not change during the entire period of the study and did not differ from the values of the negative control (Table 3). The obtained data are natural, since the collection includes herbs for which sugar-lowering properties have not been established. Horsetail grass (Edpiveii aguepvzie Negra), elderflowers (Zatbrisi Pogez), St. John's wort grass (Nuregisi

Pegra), linden flowers (Tijaye Poge5), sedum grass (Roiudopi amisshtiagie Neba), nettle leaves (Opisae ioia) are widely used in prescriptions of folk recipes of collections for the treatment of diabetes as adjuncts. Exceptions are the rhizomes and roots of delusion (Ipshaye pigotaia yei gadisev) and blueberry leaves (Mupyshii toYiit). The rhizomes and roots of oleander contain up to 44 95 inulin and mucilage, which have been experimentally proven to have a hypoglycemic effect due to the effect on glucagon-like peptide 1 (CI P-1). Increasing the level of SI P-1 by inulin, which controls more than 50 95 the insulin response to oral glucose administration, leads to an improvement in carbohydrate and lipid metabolism.

Bilberry leaves have long been used in folk medicine to lower blood sugar. But these plants make up a very small share in the total volume of the collection - one and two parts, respectively. However, the absence of a hypoglycemic effect under conditions of normal glucose homeostasis is a positive fact, since it is possible to predict a mild hypoglycemic effect of the agent in patients with diabetes, which will allow to reduce the dose of highly active synthetic hypoglycemic agents when used together with the collection and avoid the development of excessive hypoglycemic reactions (hypoglycemic commas, etc.).

Example 3. Determination of the antidiabetic effect of the antidiabetic collection on the alloxan diabetes model.

Experimental diabetes was induced by a single subcutaneous injection of alloxan at a dose of 150 mg/kg to female rats weighing 200-250 g of ISI. The decoction of the plant collection was administered intragastrically once a day in doses of 3, 6 and 9 ml/kg daily for one week before experimental diabetes simulation and for 10 days of the experiment. Control animals received water in a similar manner. Basal glycemia was determined in dynamics: initial level and after the introduction of alloxan - on the 3rd (period of acute development of hyperglycemia), on

From the 8th and 10th day.

The state of glucose homeostasis of animals against the background of model pathology was assessed by the level of basal glycemia and glucose tolerance, which was determined using the intraperitoneal glucose tolerance test (IGT).

The intraperitoneal glucose tolerance test was performed as follows: after overnight fasting (16-18 hours), rats were injected intraperitoneally with a glucose solution at a dose of 3 g/kg (2). The concentration of glucose in the blood obtained from the tail vein of animals was determined before the introduction of glucose and after 15, 45 and 60 minutes by the glucose oxidase method |21.

As an integral indicator, which makes it possible to assess the degree of development of a hyperglycemic reaction during VChTTH to the introduction of a carbohydrate solution and the effectiveness of the tool being studied, the integrated index of glycemia (IPG) was used, for which the difference between the initial values of glycemia and the values of glycemia in the corresponding period of the test was calculated , then the differences obtained at the 20th, 60th and 120th minutes of the test were summed up.

As a result of the introduction of alloxan in animals from the group of control pathology, pronounced hyperglycemia developed, which was accompanied by clinical signs of diabetes mellitus: obvious glucosuria and polyuria. On the third day of the experiment, the glucose level in the animals of this group was 5.1 times higher than the value of the intact control. The survival rate was 66.7 95 animals from the group.

On the 8th and 10th days of the experiment, the level of glycemia remained at a sufficiently high level and exceeded the basal glycemia of intact animals by 4.4 times (Table 4).

Table 4

The effect of antidiabetic drug collection on glucose homeostasis in rats with alloxan diabetes

Term 111111 Integral indicator of glycemia mmol/l (VOTTH, glucose.i | 2g/k)

Note: 7 - the differences are statistically significant in relation to the values of the intact control, p«0.05 (according to the method

Newman-Keuls); 7 - the differences are statistically significant in relation to the values of the control pathology (according to the method

Newman-Keuls); yes - the differences are statistically significant with respect to the values of collection in doses of З and б ml/kg, p«e0.05 (according to the Newman-Keuls method);

Ж - statistically significant differences compared to the values of the intact control, p«e0.05 (according to the test

And 5);

I - the differences are statistically significant in relation to the values of the control pathology, p«e0.05 (according to the test

And 50).

At the end of the experiments, the rats were decapitated under ether anesthesia, blood was collected for biochemical analysis, and the liver was resected to prepare serum and homogenates, respectively.

The content in blood serum of glucose, total cholesterol, the activity of marker enzymes of cytolysis alanine and aspartate aminotransferase (AT and A5BT) were determined according to Reitman and

Frenkel.

Determination of the level of total protein was carried out by the biuret method, urea concentration - by a color reaction with diadetylmonooxime in blood serum.

In the liver homogenate, the level of indicators reflecting the intensity of lipid peroxidation processes was studied: the end products of the degradation of unsaturated fatty acids of membrane phospholipids - TBK-reactants (TBK-R) according to the method of I.D. Stalna. |Z.

The state of the antioxidant system (AOS) was assessed by the level of reduced glutathione (С5Н) according to the method of Vepieg E.O. her a. I2| and catalase according to the method of M.A. Koroliuk. |41.

The obtained data are shown in Table 5.

Table 5

The influence of the plant collection on biochemical indicators of blood and liver homogenate of alloxandiabetic animals (Met)

Groups of animals

Indicators | ntact | control 22.0155.06

Glucose, mmol/l 6.46:0.41 26.64х5.327 21.93х4.597 (prov) 12.0342.00 t2r-0.08 73.35:52.08| 72.19-1.54 70.8054.17 67.05:2.38 12.265211 4.26-0.18 4.96:1.12 6.30-0.52 1.544-1.27 7, 12-0.38 mmol/l cholesterol, 0.9950.117 1.33:50.11

MMOl/P 1.45:20.14 (p-008) 1.2320.06 1.25:0.12 as (p-008)

AT, mmol/h-l | 050.06 | TONYA) bvezoyuv | absolutely | 05409

AVT, mmol/l | 087007 | LO | 07550023 0.8450.05 Aug 05 1.56-0.18 | 0.810.15 x 0.86-0.317 1.780, 1.77-0.267 w zhk food 44.6118.56

TBK-R, μmol/g /|31.79525.32| 63.46:8.71 16.0324.75 22.11-4 20 kg (r-0.08)

SON, μmol/g 1.42520.23 2.1550.267 1.73520.11 1.23--0.06 1.525-0.23 7

Note: 7 - the differences are statistically significant compared to the values of the intact control, p<0.05; "x - statistically significant differences in relation to the values of the control pathology, p"e0.05; their differences are statistically significant compared to the values of the comparison drug, p<0.05.

The results of the study showed that the highest effectiveness among the studied doses of the declared herbal collection was found at a dose of 9 ml/kg. Prophylactic administration of the collection decoction at a dose of 9 ml/kg helped to reduce the severity of the development of the pathology: on the 3rd day, a 1.4-fold decrease in the level of basal glycemia was observed in comparison with alloxandiabetic animals.

Under the influence of the collection at a dose of 9 ml/kg, cholesterol synthesis was restored, the manifestations of cytolysis syndrome were reduced (the activity of AIT and AB5T was significantly reduced), and the balance in the POL/AOS system was normalized (Table 5).

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Echractitepia! Oaia // MaiNnaie M. OeiiI2eppe", Raigise O. Sapi apa Atsageu M. MeugipsK // Ategisap

Zosieiu Togo Myshiopo U. Miy.-2007.- Moi. 137- R 25475-25515. Mode of assessment: pEruLp.pshyiop.Ogo/sdi/sopiepiyI1 37/ 1/25475.

Z. Horbenko N.M. Metformin - the drug of choice for preventing and reducing the severity of cardiovascular pathology in patients with type 2 diabetes / N.Y.

Horbenko, M.Yu. Horshunskaya // Ukr. medical journal. - 2005. - Mo 4 (48). - P. 29-35. 4. Siobra! rgemaiepse oi aiareiev: eviitayez og She uyeag 2000 apa rgo|esiyopv Tog 2030 / 5. Ma, a. Vodiis, A. Stgeep (ей а/.| // Oiabeiez Sage. - 2004. - Moi. 27, Mo 5. - R. 1047-1053.

Claims (1)

USEFUL MODEL FORMULA A collection of medicinal plants for the treatment of patients with diabetes mellitus type II, which includes horsetail 25 field grass, St. John's wort, blueberry leaves, which is distinguished by the fact that it additionally contains elder flower, delusion rhizomes and roots, linden flower, sedge grass, nettle leaves, and all ingredients are taken in the following ratio, mass. h.: horsetail grass 2.0 St. John's wort grass 1.0 blueberry leaves 3.0 elder flowers 1.0 delusion rhizomes and roots 1.0 linden flowers 1.0 spurge grass 1.0 nettle leaves 1.0.