UA10385U - СПОСОБ ДИАГНОСТИКИ Ph' ЛЕЙКЕМИЙ С ПОМОЩЬЮ СПЕЦИФИЧЕСКИХ ПРАЙМЕРОВ - Google Patents
СПОСОБ ДИАГНОСТИКИ Ph' ЛЕЙКЕМИЙ С ПОМОЩЬЮ СПЕЦИФИЧЕСКИХ ПРАЙМЕРОВ Download PDFInfo
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Abstract
Способ диагностики Ph' лейкемий с помощью специфических праймеров включает в себя операции получения образца крови больного, приготовления реакционной смеси для синтеза кДНК, которая содержит РНК, соответствующий праймер, обратную транскриптазу M-MLV (GibcoBRL), PHазин и dNTP и буфер для обратной транскриптазы, проведение синтеза при температуре +36,8...+37,2°С на протяжении 50-70 минут, использование аликвоты реакционной смеси для проведения амплификации. При этом на первом этапе проводят серию циклов полимеразной цепной реакции с использованием соответствующих праймеров при заданном температурном режиме синтеза. Далее проводят второй этап полимеразной цепной реакции с использованием соответствующих праймеров при заданном температурном режиме, а продукты амплификации анализируют в агарозном геле и прогнозируют дальнейшее течение заболевания. В качестве праймеров при приготовлении реакционной смеси для синтеза кДНК используют, соответственно, Phl-f (5'-GTCTTCCTGTTCACCGAGCTG-3') и Phl-r (5'-GCTTAGAGTGTTATCTCCACTGGC-3'), а на втором этапе полимеразной цепной реакции используют праймеры Ph-f (5'-CTGCAAATGGTACATTCCGCTCAC-3') и Ph-r (5'-AACGAGCGGCTTCACTCAGACC-3').
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| UAU200503775U UA10385U (ru) | 2005-04-20 | 2005-04-20 | СПОСОБ ДИАГНОСТИКИ Ph' ЛЕЙКЕМИЙ С ПОМОЩЬЮ СПЕЦИФИЧЕСКИХ ПРАЙМЕРОВ |
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| Application Number | Priority Date | Filing Date | Title |
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| UAU200503775U UA10385U (ru) | 2005-04-20 | 2005-04-20 | СПОСОБ ДИАГНОСТИКИ Ph' ЛЕЙКЕМИЙ С ПОМОЩЬЮ СПЕЦИФИЧЕСКИХ ПРАЙМЕРОВ |
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| Publication Number | Publication Date |
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| UA10385U true UA10385U (ru) | 2005-11-15 |
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| UAU200503775U UA10385U (ru) | 2005-04-20 | 2005-04-20 | СПОСОБ ДИАГНОСТИКИ Ph' ЛЕЙКЕМИЙ С ПОМОЩЬЮ СПЕЦИФИЧЕСКИХ ПРАЙМЕРОВ |
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| UA (1) | UA10385U (ru) |
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