TWM623529U - Cryogenic vial - Google Patents

Abstract

A freezing tube includes a tube body, a plug body, a fixing piece and a cover. The pipe body has a cavity, and the first end of the pipe body is provided with an opening. The plug body is arranged at the first end of the tube body, and its axis is aligned with the axis of the tube body, and includes a sealing part, a puncturing part and a guiding part. The point is located at the center of the top end of the piercing part and is aligned with the axis of the plug body, the guide part is arranged at the bottom end of the sealing part, located in the cavity, and its outer diameter tapers away from the sealing part. The fixing piece is arranged on the first end of the pipe body, fixes the sealing part on the first end of the pipe body, and has a perforation, and the puncturing part is embedded in the perforation. The cover is arranged on the fixing element and closes the perforation. Thereby, the present invention allows the plug body to enter the chamber smoothly and the needle is easy to align with the center of the plug body.

Description

cryovials

This creation is about a tube body, especially a cryopreservation tube for preserving cells and other biologically related materials.

Biomedical research usually starts from cell experiments, and after the cell experiments obtain preliminary research results, they will enter animal experiments and clinical trials. Therefore, in vitro cultured cell lines are one of the indispensable research materials in biomedical experiments, and the preservation of cells is particularly important.

In addition, cell therapy is an emerging therapy applied in cancer treatment and regenerative medicine. In terms of cancer treatment, the number of immune cells in patients can be increased by culturing cells in vitro, and then the immune cells of patients can be injected into the body of patients, and finally cancer cells can be killed by the increased immune cells. In regenerative medicine, the differentiation and proliferation properties of stem cells are used to repair the organs or tissues of patients. Since cell therapy is administered to the patient's body, the preservation of cells needs to be more careful.

Liquid nitrogen cryopreservation is currently the most commonly used cell cryopreservation technique. Healthy cells are placed in cryovials and stored in liquid nitrogen barrels.

It should be noted that cells are most likely to be contaminated during the process of loading or removing cells from cryovials, and the whole process needs to be kept sterile. Moreover, because cells are very precious, all cells in the cryovial are usually not taken out at one time, so they will be taken in batches. Therefore, users usually operate in a biological safety cabinet, and the operation process must be very careful to prevent the cells from being contaminated by microorganisms. At the same time, attention should be paid to the convenience of taking the cells to ensure that the cells will not be contaminated by microorganisms due to multiple batches.

A conventional cryopreservation tube includes a tube body and a cover. The cover is usually quite tightly plugged into the opening of the tube body to ensure that the chamber of the tube body is completely sealed and prevent the cells from being contaminated by microorganisms. Therefore, it is rather difficult to remove the cap from the tube body when loading or accessing cells. Furthermore, after the cover is removed, the chamber of the tube body is directly communicated with the external space. Although the cells can be easily loaded or taken out, the cells are easily contaminated by microorganisms.

Another conventional cryopreservation tube includes a tube body, a stopper body and a lid. In the process of loading the cells, the freezing medium containing the cells is firstly loaded into the chamber of the tube body, then the plug body closes the opening of the tube body, and finally the cap is covered with the plug body. In the process of taking cells, first remove the cap from the plug body, and then pass the needle through the plug body into the chamber of the tube body to take the cells. At this time, the plug body can ensure that the chamber of the tube body is kept at A closed state without communication with the outside space to prevent the cells from being contaminated by microorganisms.

However, during the assembly process, since the outer diameter of the plug body is substantially equal to the inner diameter of the tube body, it takes considerable force to close the opening of the tube body with the plug body. It should be noted that pushing the plug with excessive force will cause the plug body to deform or even break. A gap will be formed between the deformed or damaged plug body and the inner wall of the tube body, and the gap will become a channel for microorganisms to enter the chamber, causing the cells to be contaminated by microorganisms.

Furthermore, it is quite difficult to align the needle with the center of the plug body, which often results in the needle piercing the plug body from a position offset from the center of the plug body and failing to pass through the plug body along the axis of the plug body. It should be noted that because the plug body and the tube body are substantially coaxial, after the needle enters the chamber of the tube body, the position of the needle is also deviated from the axis of the tube body, so it is quite close to the inner wall of the tube body, causing the needle to be slightly skewed. damage to the inner wall of the tube.

The main purpose of this creation is to provide a cryopreservation tube, which can allow the plug body to enter the chamber through the opening of the first end of the tube body smoothly, so as to prevent the plug body from being deformed and damaged.

Another object of the present invention is to provide a cryopreservation tube, which can allow the needle to be easily aligned with the center of the plug body and remain on the axis of the plug body and the tube body after passing through the plug body, and the needle will not collide with the tube at all The inner wall of the body can thus remain unscathed.

In order to achieve the aforementioned purpose, the present invention provides a cryopreservation tube, which includes a tube body, a stopper body, a fixing member and a cover. The pipe body has a first end, a second end and a cavity. The first end of the pipe body is provided with an opening, and the opening communicates with the cavity. The plug body is arranged at the first end of the tube body, its axis is aligned with an axis of the tube body, and includes a sealing part, a puncturing part and a guiding part, the sealing part closes the opening, and the puncturing part is arranged on a part of the sealing part. The top end has an aiming point, the aiming point is located at the center of a top end of the piercing part and is aligned with the axis of the plug body, the guiding part is arranged at a bottom end of the sealing part, located in the chamber, and its outer diameter is away from the sealing part direction is tapered. The fixing piece is arranged on the first end of the pipe body, fixes the sealing part on the first end of the pipe body, and has a perforation, and the puncturing part is embedded in the perforation. The cover is arranged on the fixing member and closes the perforation.

In some embodiments, the plug body further defines a concave portion, and the concave portion sequentially penetrates the interior of the sealing portion and the interior of the guide portion from a bottom end of the guide portion and extends to the interior of the puncture portion.

In some embodiments, an inner diameter of the concave portion increases gradually from the inside of the piercing portion to the bottom end of the guiding portion.

In some embodiments, an axis of the recess is aligned with the axis of the plug body.

In some embodiments, the bottom end of the guide portion is arc-shaped.

In some embodiments, the guide portion is in the shape of a truncated cone.

In some embodiments, a protruding portion is protruded from the top of the piercing portion, and the aiming point is located at the center of the protruding portion.

In some embodiments, the protruding portion defines two grooves, and an intersection of the grooves is located at the center of the protruding portion and defines an aiming point.

In some embodiments, the grooves are straight and perpendicular to each other and their centers are located at the centers of the protrusions.

In some embodiments, the grooves are straight and perpendicular to each other, the center of one of the grooves is located at the center of the protrusion, and one end of the other groove is located at the center of the protrusion.

In some embodiments, a top end of the piercing portion is aligned with a top end of the fastener, and the protruding portion is located outside the perforation.

In some embodiments, the material of the plug body is a polymer elastic material.

In some embodiments, a flange is disposed around the outer side of the sealing portion, a bottom end of the flange abuts an end surface of the first end of the pipe body, and the fixing member abuts a top end of the flange.

In some embodiments, the fixing member includes a screwing part, a positioning part and a combining part, the screwing part is screwed on the outer side of the first end of the pipe body, the positioning part is connected between the screwing part and the combining part and The sealing part is fixed on the first end of the pipe body, the joint part is provided with a perforation, and the cover is combined with the joint part.

In some embodiments, the cover includes a ring portion and a top cover, the ring portion forms a groove, the joint portion is embedded in the groove, the top cover is disposed at a top end of the ring portion, and its outer diameter is larger than that of the ring portion. outer diameter.

In some embodiments, the outer side of the first end of the pipe body has an external thread and a ratchet wheel, the ratchet wheel is located below the external thread, the ratchet wheel includes a plurality of ratchet teeth, and an extending direction of the external thread is opposite to an extending direction of each ratchet tooth ; And wherein, the inner side of the threaded portion has an inner thread, a bottom end of the threaded portion is surrounded by a plurality of pawls, and an extension direction of the inner thread is opposite to an extension direction of each pawl; During the process of connecting to the outer side of the first end of the pipe body, through the screw connection between the inner thread and the outer thread, the threaded portion can rotate relative to the pipe body along a first direction and from the first end of the pipe body toward the pipe body move in the direction of the second end of the tube until the pawls engage with the ratchet teeth; after the screwing part is screwed with the outer side of the first end of the tube body, the positioning part fixes the sealing part on the first end of the tube body The ratchet can prevent the screwed part from rotating relative to the pipe body along a second direction, so that the screwed part is firmly fixed on the first end of the pipe body.

The effect of the present invention is that the plug body can smoothly enter the chamber through the opening of the first end of the pipe body through the special configuration of the guide portion, so as to prevent the plug body from being deformed and damaged.

Furthermore, the present invention can easily align the needle with the center of the plug body through the aiming point and keep it on the axis of the plug body and the tube body after passing through the plug body, and the needle will not collide with the inner wall of the tube body at all. , so it can remain unscathed.

The embodiments of the present invention will be described in more detail below with reference to the drawings and component symbols, so that those skilled in the art can implement them after studying the description.

Please refer to FIG. 1 to FIG. 9 , the present invention provides a cryopreservation tube including a tube body 10 , a plug body 20 , a fixing member 30 and a cover 40 .

As shown in FIG. 2 and FIG. 9 , the tube body 10 has a first end 11 , a second end 12 and a cavity 13 . The first end 11 of the tube body 10 defines an opening 111 , and the opening 111 communicates with the chamber 13 .

As shown in FIGS. 2 , 3 , 4A, 4B and 9 , the plug body 20 is disposed at the first end 11 of the tube body 10 , its axis 201 is aligned with an axis 101 of the tube body 10 , and includes a sealing portion 21. A puncturing part 22 and a guiding part 23. The sealing portion 21 closes the opening 111 . The piercing part 22 is disposed at a top end of the sealing part 21 and has an aiming point 221 , which is located at the center of a top end of the piercing part 22 and is aligned with the axis 201 of the plug body 20 . The guide portion 23 is disposed at a bottom end of the sealing portion 21 in the chamber 13 , and its outer diameter tapers away from the sealing portion 21 .

As shown in FIG. 1 , FIG. 2 , FIG. 5 , FIG. 6 , FIG. 7 and FIG. 9 , the fixing member 30 is arranged on the first end 11 of the pipe body 10 to fix the sealing part 21 on the first end 11 of the pipe body 10 , And has a perforation 331 ; the puncture part 22 is embedded in the perforation 331 .

As shown in FIG. 1 , FIG. 2 , FIG. 8 and FIG. 9 , the cover 40 is disposed on the fixing member 30 and closes the through hole 331 .

The following will further illustrate how cells are loaded into the cryopreservation tube of the present invention in conjunction with the diagrams in Figure 2 and Figure 9 .

First, the immune cells or stem cells are taken out from the human body for cell culture; after the cell culture is completed, the freezing medium containing the cells is drawn into the chamber 13 . Next, through the special configuration such as the outer diameter of the guiding portion 23 tapering in the direction away from the sealing portion 21, the guiding portion 23 can smoothly enter the chamber 13 through the opening 111 of the first end 11 of the tubular body 10; After the guide portion 23 enters the chamber 13 , the user can insert the sealing portion 21 of the plug body 20 on the first end 11 of the tube body 10 and close the opening 111 with a slight force. Then, the fixing member 30 fixes the sealing portion 21 on the first end 11 of the tube body 10 , and at the same time, the piercing portion 22 of the plug body 20 enters and is embedded in the through hole 331 . Next, the cover 40 is disposed on the fixing member 30 and closes the perforation 331 . Finally, the cryopreservation tube of this creation is gradually cooled down according to the procedure of the cryopreservation method, and then placed in a liquid nitrogen barrel for long-term storage.

FIG. 10 shows a schematic diagram of the needle 100 aligning with the aiming point 221 of the piercing portion 22 of the plug body 20 of the present invention, and FIG. 11 shows a schematic diagram of the needle 100 entering the tube body 10 of the present invention. The following will further describe how to take out the cells from the cryopreservation tube of the present invention in conjunction with the diagrams in Fig. 9 and Fig. 10 .

水浴槽中將本創作的凍存管解凍，然後將解凍後的本創作的凍存管移入生物安全櫃中。接著，如圖10所示，將封蓋40從固定件30上移除，使得穿刺部22能夠透過穿孔331顯露於外，使用者能夠將針100對準瞄準點221。然後，如圖11所示，針100通過瞄準點221以後會沿著塞體20的軸線201依序穿過穿刺部22、密封部21和導引部23進入腔室13中，針100在腔室13中會沿著管體10的軸線101至管體10的第二端12，以抽取含細胞的冷凍培養基。在抽取細胞的過程中，針100始終受限於塞體20而保持在塞體20的軸線201和管體10的軸線101上；即使針100略為偏斜，針100依舊受限於塞體20而不會脫離塞體20的軸線201和管體10的軸線101太遠，針100始終距離管體10的內壁甚遠；因此，針100完全不會碰撞到管體10的內壁，因而能夠保持毫髮無損的狀態。抽取出的含細胞的冷凍培養基經由細胞培養的程序能夠獲得充足的健康細胞，這些健康的細胞能夠進一步應用於生物醫學實驗或細胞治療。 First, remove the cryovial of this creation from the liquid nitrogen tank, at 37

Thaw the cryotubes of this creation in a water bath, and then move the thawed cryotubes of this creation into a biological safety cabinet. Next, as shown in FIG. 10 , the cover 40 is removed from the fixing member 30 , so that the piercing portion 22 can be exposed through the perforation 331 , and the user can align the needle 100 with the aiming point 221 . Then, as shown in FIG. 11 , after passing through the aiming point 221 , the needle 100 will pass through the puncturing part 22 , the sealing part 21 and the guiding part 23 in sequence along the axis 201 of the plug body 20 and enter the cavity 13 , where the needle 100 is in the cavity 13 . The chamber 13 will be along the axis 101 of the tube body 10 to the second end 12 of the tube body 10 in order to withdraw the freezing medium containing cells. During the process of extracting cells, the needle 100 is always limited by the plug body 20 and remains on the axis 201 of the plug body 20 and the axis 101 of the tube body 10; even if the needle 100 is slightly deflected, the needle 100 is still limited by the plug body 20 Without being too far away from the axis 201 of the plug body 20 and the axis 101 of the tube body 10, the needle 100 is always far away from the inner wall of the tube body 10; therefore, the needle 100 will not hit the inner wall of the tube body 10 at all, so it can Stay unscathed. The extracted cell-containing freezing medium can obtain sufficient healthy cells through the cell culture procedure, and these healthy cells can be further used in biomedical experiments or cell therapy.

In addition, during the process of extracting cells, the fixing member 30 can ensure that the plug body 20 is fixed at the first end 11 of the tube body 10 and closes the opening 111 , so that the chamber 13 is kept in a sealed state without being communicated with the external space. Therefore, the cells in the chamber 13 are not contaminated by microorganisms.

In addition, after the procedure of extracting cells is completed, there are still remaining cells in the chamber 13 that can be used in batches in the future. However, a hole in the plug body 20 has been pierced by the needle 100, and the hole communicates with the cavity 13, and the cavity 13 can communicate with the external space through the hole and is no longer in a closed state. At this time, as long as the cover 40 is installed on the fixing member 30 again, the cover 40 can seal the hole, so as to prevent microorganisms from entering the chamber 13 through the hole. Finally, the cryopreservation tube of this creation can be gradually cooled down according to the procedure of the cryopreservation method, and then placed in a liquid nitrogen barrel for long-term storage.

As shown in FIG. 3 and FIG. 9 , in a preferred embodiment, the plug body 20 further defines a concave portion 24 , and the concave portion 24 penetrates the interior of the sealing portion 21 and the guiding portion 23 from a bottom end of the guiding portion 23 in sequence. inside and extends to the inside of the piercing portion 22 . That is to say, the recessed portion 24 does not completely penetrate the puncturing portion 22 and becomes a blind hole. In this way, the existence of the concave portion 24 can reduce the obstacles that the needle 100 needs to break through to only the puncture portion 22, and the user can make the needle 100 pierce the puncture portion 22 to reach the concave portion 24 and from the puncture portion only by exerting some force. 22 is removed, which is quite labor-saving.

As shown in FIG. 9 , preferably, an inner diameter of the recessed portion 24 increases gradually from the inside of the puncturing portion 22 to the bottom end of the guiding portion 23 . Therefore, a thickness of the sealing portion 21 is still sufficient to prevent distortion and damage when the sealing portion 21 is embedded in the first end 11 of the tube body 10 .

As shown in FIG. 9 , in the preferred embodiment, an axis 241 of the recessed portion 24 is aligned with the axis 201 of the plug body 20 . Therefore, the needle 100 will move substantially along the axis 241 of the recess 24 after passing the aiming point 221 . During the process of extracting cells, the needle 100 is always limited by the puncture portion 22 and kept on the axis 241 of the concave portion 24; even if the needle 100 is slightly deflected, the needle 100 is still limited by the puncture portion 22 and will not escape from the concave portion 24 If the axis 241 is too far away, the needle 100 is always far away from the inner wall of the concave portion 24; therefore, the needle 100 will not collide with the inner wall of the concave portion 24 at all, and thus can remain unharmed.

As shown in FIG. 2 and FIG. 9 , preferably, the bottom end of the guide portion 23 is in an arc shape. Therefore, the bottom end of the arc-shaped guide portion 23 can smoothly enter the chamber 13 through the opening 111 of the first end 11 of the pipe body 10 .

As shown in FIG. 2 and FIG. 9 , preferably, the guide portion 23 is in the shape of a truncated cone. Therefore, by virtue of the special configuration such as the truncated cone shape of the guide portion 23 , the guide portion 23 can smoothly enter the chamber 13 through the opening 111 of the first end 11 of the tube body 10 .

As shown in FIG. 2 , FIG. 4A and FIG. 4B , in a preferred embodiment, a protruding part 222 is protruded from the top of the puncturing part 22 , and the aiming point 221 is located at the center of the protruding part 222 . Since the protruding portion 222 has a certain thickness, it is only necessary to form the aiming point 221 in the center of the protruding portion 222 .

As shown in FIGS. 4A and 4B , preferably, the protruding portion 222 defines two grooves 223 and 224 , and an intersection of the grooves 223 and 224 is located in the center of the protruding portion 222 and defines an aiming point 221 . Since the protrusion 222 has a certain thickness, as long as the grooves 223 and 224 are cut on the protrusion 222 , the grooves 223 and 224 can easily meet at the center of the protrusion 222 to form the aiming point 221 . In this way, in addition to being easy to manufacture and avoiding puncturing the puncturing part 22 , the user can also easily find the position of the aiming point 221 .

As shown in FIG. 4A , in one embodiment of the plug body 20 , the grooves 223 and 224 are linear and perpendicular to each other, and their centers are located at the center of the protruding portion 222 . In other words, the combination of the grooves 223, 224 appears as a cross. As shown in FIG. 4B , in another embodiment of the plug body 20 , the grooves 223 and 224 are linear and perpendicular to each other, the center of the groove 223 is located at the center of the protruding portion 222 , and one end of the groove 224 is located at the center of the protrusion 222 . the center of the protrusion 222 . In other words, the combination of the grooves 223, 224 appears T-shaped. However, the combined shape of the grooves 223 and 224 is not limited thereto, and any geometric shape capable of forming the aiming point 221 can be the combined shape of the grooves 223 and 224 .

In terms of manufacturing, the combination of the cross-shaped and T-shaped grooves 223, 224 is the easiest to form compared to other shapes. Among them, the combination of the cross-shaped grooves 223 and 224 is the most accurate in forming the aiming point 221 .

In terms of use, compared with other shapes, the combination of the cross-shaped and T-shaped grooves 223 and 224 is the easiest to find the position of the aiming point 221 . Among them, the combination of the cross-shaped grooves 223 and 224 can best allow the user to see the position of the aiming point 221 at a glance.

As shown in FIG. 9 , preferably, the top end of the piercing portion 22 is aligned with a top end of the fixing member 30 , and the protruding portion 222 is located outside the through hole 331 . As shown in FIG. 10 , after the cover 40 is removed from the fixture 30 , the protrusions 222 are clearly exposed, and the user can easily see the aiming points 221 on the protrusions 222 and align the needle 100 with the aiming points 221 .

Preferably, the material of the plug body 20 is a polymer elastic material. The polymer elastic material has elasticity and can be slightly deformed, so that the sealing portion 21 is easily embedded in the first end 11 of the tube body 10 , and the puncture portion 22 is easily embedded in the through hole 331 . In addition, the polymer elastic material has the advantage of being easily punctured by the needle 100 , and the user can let the needle 100 puncture the puncture portion 22 to reach the concave portion 24 and move out from the puncture portion 22 only by exerting some force, which is quite labor-saving. In addition, after being punctured by the needle 100, the puncture portion 22 can also wrap the needle 100 with its elastic deformation ability, so as to prevent microorganisms from entering the chamber 13 from the hole. The polymer elastic material is preferably silica gel, but not limited thereto, any elastic material can be used as the polymer elastic material and is covered by the scope of the present invention.

As shown in FIG. 2 , FIG. 3 , FIG. 4A , FIG. 4B and FIG. 9 , in a preferred embodiment, a flange 211 is arranged around the outer side of the sealing portion 21 , and a bottom end of the flange 211 abuts against the pipe body 10 On one end face of the first end 11 of the flange 211 , the fixing member 30 abuts against a top end of the flange 211 . After the sealing part 21 is embedded in the first end 11 of the tube body 10 , or when the user applies force to the needle 100 to puncture the puncturing part 22 , the flange 211 can prevent the sealing part 21 from following the inner wall of the tube body 10 Move toward the second end 12 of the body 10 .

As shown in FIGS. 1 , 2 , 5 , 6 and 9 , in a preferred embodiment, the fixing member 30 includes a screwing portion 31 , a positioning portion 32 and a joint portion 33 . The screwing portion 31 is screwed to the outer side of the first end 11 of the tube body 10 , so that the fixing member 30 is fixed on the first end 11 of the tube body 10 . The positioning part 32 is connected between the screwing part 31 and the joint part 33 and abuts against the top end of the flange 211 , so that the sealing part 21 is fixed on the first end 11 of the pipe body 10 . A through hole 331 is formed in the joint portion 33 . Therefore, the coupling portion 33 can substantially restrict the puncturing portion 22 to prevent the puncturing portion 22 from being deformed when subjected to force. The cover 40 is coupled to the coupling portion 33 . Therefore, the cover 40 can be easily installed and removed from the fixing member 30 .

Preferably, the outer side of the screwing portion 31 has a plurality of ribs 311 , and the ribs 311 are arranged at intervals along a circumferential direction. Thereby, the ribs 311 can increase the anti-slip effect, so that the user's fingers can be easily grasped, so as to prevent the user from slipping and knocking down the tube body 10 .

As shown in FIGS. 1 , 2 , 8 and 9 , in a preferred embodiment, the cover 40 includes a ring portion 41 and a top cover 42 . The ring portion 41 forms a groove 411 , and the coupling portion 33 is embedded in the groove 411 . The top cover 42 is disposed on a top end of the ring portion 41 , and its outer diameter is larger than that of the ring portion 41 . Therefore, the outer periphery of the top cover 42 is relatively protruding relative to the ring portion 41 , and the user can easily move the top cover 42 by grasping the outer periphery of the top cover 42 , thereby allowing the ring portion 41 to be easily disengaged from the joint portion 33 . After the cells are loaded into the chamber 13, the top cover 42 together with the ring portion 41 closes the perforation 331. After the procedure of extracting cells is completed, the top cover 42 abuts against a top of the protruding portion 222 , so as to close the hole and prevent microorganisms from entering the chamber 13 through the hole.

Preferably, a ring groove 412 is formed on the inner wall of the ring portion 41 , and a convex ring 332 is protruded from the outer wall of the joint portion 33 . In other embodiments, the ring groove 412 may also be formed on the outer wall of the joint portion 33 , and the protruding ring 332 may also be protruded on the inner wall of the ring portion 41 .

Preferably, an inner side of a bottom end of the ring portion 41 has a chamfer 413 . Accordingly, when the bottom end of the ring portion 41 contacts the top end of the joint portion 33, the ring portion 41 can be smoothly aligned by the chamfer 413 and pass through the outside of the joint portion 33, and the ring portion 41 then slides down to make the The coupling portion 33 is embedded in the groove 411 .

As shown in FIGS. 1 , 2 , 5 , 6 , 7 and 9 , the outer side of the first end 11 of the pipe body 10 has an external thread 14 and a ratchet 15 , and the ratchet 15 is located below the external thread 14 and Including a plurality of ratchet teeth 151, an extension direction of the external thread 14 is opposite to an extension direction of each ratchet tooth 151; the inner side of the screw connection part 31 has an inner thread 313, and a bottom end of the screw connection part 31 is surrounded by a plurality of ratchet pawls 312 , an extension direction of the inner thread 313 is opposite to an extension direction of each pawl 312 . As shown in FIG. 2 , in the process of screwing the screwing portion 31 to the first end 11 of the pipe body 10 , the screwing portion 31 can be along a first direction A by screwing the inner thread 313 with the outer thread 14 . Rotate relative to the tube body 10 and move from the first end 11 of the tube body 10 toward the second end 12 of the tube body 10 until the pawls 312 engage with the ratchet teeth 151 . After the screwing part 31 is screwed with the outer side of the first end 11 of the tube body 10, the positioning part 32 fixes the sealing part 21 on the first end 11 of the tube body 10, and the ratchet 15 can prevent the screwing part 31 from moving along a The second direction B (ie, the opposite direction of the first direction A) rotates relative to the tube body 10 , so that the screwing portion 31 is firmly fixed on the first end 11 of the tube body 10 . Accordingly, the fixing member 30 and the plug body 20 will not be separated from the tube body 10 at all, so as to prevent the cells from being contaminated by microorganisms.

As shown in FIG. 11 , the second end 12 of the pipe body 10 includes a conical portion 121 and a cylindrical portion 122 . The conical portion 121 closes the chamber 13 and is recessed toward the direction away from the first end 11 of the tube body 10 , so that cells located at a bottom of the chamber 13 are concentrated in the middle of the conical portion 121 , so that the needle 100 can follow the tube body 10 The axis 101 moves to the middle of the cone 121 to extract the cells. The cylindrical portion 122 is arranged around the outer side of the conical portion 121 , and can stand on the table to prevent the tube body 10 from falling.

The above embodiment takes the preservation of human immune cells or stem cells as an example, but is not limited to this, any biological material (such as vector, plastid, bacteriophage, virus, bacteria, fungus, animal or plant cell strain, animal or plant tissue Cultures, protozoa, unicellular algae, etc.), nucleic acids and proteins, etc., can all be put into the cryopreservation tube of the present invention for cryopreservation. Cryopreservation is not limited to the environment of liquid nitrogen, but can also be an environment below zero degrees, depending on the optimal storage temperature required for the stored substances.

The above descriptions are only used to explain the preferred embodiments of this creation, and are not intended to limit this creation in any form. Therefore, any modifications or changes to this creation are made in the same creative spirit. , should still be included in the scope of protection intended for this creation.

10: Tube body 101: Axis 11: First End 111: Opening 12: Second end 121: Conical part 122: barrel 13: Chamber 14: External thread 15: Ratchet 151: Ratchet 20: Plug body 201: Axis 21: Sealing part 211: Flange 22: Puncture part 221: Aiming Point 222: Protrusion 223, 224: Groove 23: Guidance Department 24: Depression 241: Axis 30: Fixtures 31: Screw part 311: Ribs 312: Pawl 313: Internal thread 32: Positioning part 33: Joint 331: Perforation 332: convex ring 40: capping 41: Ring Department 411: Groove 412: Ring groove 413: Chamfer 42: top cover 100: Needle A: The first direction B: The second direction

[Fig. 1] A side view of the cryotube of this creation. [Fig. 2] An exploded view of the cryopreservation tube of this creation. [Fig. 3] A bottom view of the plug body of this creation. [ FIG. 4A ] A plan view of one embodiment of the plug body of the present invention. [ FIG. 4B ] A plan view of another embodiment of the plug body of the present invention. [Fig. 5] is a bottom view of the fixture of this creation. [FIG. 6] A plan view of the fixture of this creation. [Fig. 7] is a cross-sectional view of the fastener of the present invention. [Fig. 8] A bottom view of the cover of this creation. [Fig. 9] A cross-sectional view of the cryopreservation tube of the present invention. [ FIG. 10 ] A schematic diagram showing the aiming point of the piercing portion of the plug body aligned with the present invention. [Fig. 11] shows a schematic diagram of the needle entering the body of the present creation.

10: Tube body

15: Ratchet

31: Screw part

311: Ribs

40: capping

41: Ring Department

42: top cover

Claims (16)

A cryopreservation tube comprising: a pipe body having a first end, a second end and a chamber, the first end of the pipe body defines an opening, and the opening communicates with the chamber; A plug body is disposed at the first end of the tube body, its axis is aligned with an axis of the tube body, and includes a sealing part, a puncturing part and a guiding part, the sealing part closes the opening, the The piercing part is arranged at a top end of the sealing part and has an aiming point, the aiming point is located in the center of a top end of the piercing part and is aligned with the axis of the plug body, and the guiding part is arranged at a bottom of the sealing part an end located in the chamber and having an outer diameter that tapers away from the seal; a fixing member disposed on the first end of the tube body, the sealing portion is fixed on the first end of the tube body, and has a through hole, and the piercing portion is embedded in the through hole; and A cover is arranged on the fixing member and closes the perforation. The cryopreservation tube according to claim 1, wherein the plug body further defines a concave portion, and the concave portion sequentially penetrates the interior of the sealing portion and the interior of the guide portion from a bottom end of the guide portion and extends to inside the puncture part. The cryopreservation tube of claim 2, wherein an inner diameter of the concave portion increases gradually from the inside of the puncturing portion to the bottom end of the guide portion. The cryopreservation tube of claim 2, wherein an axis of the recess is aligned with the axis of the plug body. The cryopreservation tube according to claim 2, wherein the bottom end of the guide portion is arc-shaped. The cryopreservation tube according to claim 1, wherein the guide portion is in the shape of a truncated cone. The cryopreservation tube of claim 1, wherein a protruding portion is protruded from the top of the puncturing portion, and the aiming point is located at the center of the protruding portion. The cryopreservation tube of claim 7, wherein the protruding portion defines two grooves, and an intersection of the grooves is located in the center of the protruding portion and defines the aiming point. The cryopreservation tube of claim 8, wherein the grooves are all straight and perpendicular to each other and their centers are all located at the center of the protruding portion. The cryopreservation tube of claim 8, wherein the grooves are all straight and perpendicular to each other, the center of one of the grooves is located at the center of the protrusion, and the other of the grooves is located at the center of the protrusion. One end is located in the center of the protrusion. The cryopreservation tube of any one of claims 7 to 10, wherein the top end of the piercing portion is aligned with a top end of the fixing member, and the protruding portion is located outside the perforation. The cryopreservation tube according to claim 1, wherein the material of the plug body is a polymer elastic material. The cryopreservation tube of claim 1, wherein a flange is arranged around the outer side of the sealing portion, a bottom end of the flange abuts against an end face of the first end of the tube body, and the fixing member abuts against against a top end of the flange. The cryopreservation tube according to claim 1, wherein the fixing member comprises a screwing part, a positioning part and a connecting part, the screwing part is screwed on the outer side of the first end of the tube body, and the positioning part is The sealing part is connected between the screwing part and the joint part, and the sealing part is fixed on the first end of the pipe body, the joint part has the through hole, and the cover is combined with the joint part. The cryopreservation tube of claim 14, wherein the cover comprises a ring portion and a top cover, the ring portion forms a groove, the joint portion is embedded in the groove, and the top cover is disposed in the ring A top end of the ring portion, and its outer diameter is larger than the outer diameter of the ring portion. The cryopreservation tube of claim 14, wherein the outer side of the first end of the tube body has an external thread and a ratchet wheel, the ratchet wheel is located below the external thread, the ratchet wheel comprises a plurality of ratchet teeth, and the external thread An extension direction of each of the ratchet teeth is opposite to an extension direction of each of the ratchet teeth; and wherein, the inner side of the threaded portion has an inner thread, a bottom end of the threaded portion is surrounded by a plurality of pawls, and an extension direction of the inner thread is Opposite to an extension direction of each of the pawls; wherein, in the process of the screw connection portion being screwed on the outside of the first end of the pipe body, by the screw connection between the inner thread and the outer thread, the screw The joint can rotate relative to the pipe body along a first direction and move from the first end of the pipe body toward the second end of the pipe body until the pawls engage with the ratchet teeth; in the After the screwing part is screwed with the outer side of the first end of the pipe body, the positioning part fixes the sealing part on the first end of the pipe body, and the ratchet can prevent the screwing part from following a second The direction is rotated relative to the pipe body, so that the threaded portion is firmly fixed on the first end of the pipe body.

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