TWM506283U - Fast three-dimensional conjugate focus spectrum imaging device - Google Patents

Fast three-dimensional conjugate focus spectrum imaging device Download PDF

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Publication number
TWM506283U
TWM506283U TW104205104U TW104205104U TWM506283U TW M506283 U TWM506283 U TW M506283U TW 104205104 U TW104205104 U TW 104205104U TW 104205104 U TW104205104 U TW 104205104U TW M506283 U TWM506283 U TW M506283U
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light
light source
test sample
fast
imaging device
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TW104205104U
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Chinese (zh)
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Jing-Xiang Chen
yao-hong Guo
yu-xuan Guo
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Ching Yeh Sheng Entpr Co Ltd
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快速三維共軛焦光譜成像裝置Fast three-dimensional conjugate focal length imaging device

本創作係有關於一種快速三維共軛焦光譜成像裝置。This creation is about a fast three-dimensional conjugate focal-spectrum imaging device.

快速三維共軛焦光譜成像裝置可應用於材料之結構分析、缺陷分析、鍵結分析等,可被分析的材料包括太陽光電材料、光電半導體材料、高分子材料等,習知的共軛焦光譜成像裝置通常只能針對測試樣品之某一特定點(一維)進行測試,如需進行較大範圍(二維或三維)測試則需花費許多時間反覆測量,才能完成所需測試。The fast three-dimensional conjugate focal-spectrum imaging device can be applied to structural analysis, defect analysis, bonding analysis, etc. of materials, and materials that can be analyzed include solar photovoltaic materials, photoelectric semiconductor materials, polymer materials, etc., conventional conjugated focal spectroscopy Imaging devices can usually only be tested at a specific point (one-dimensional) of the test sample. If a large-scale (two-dimensional or three-dimensional) test is required, it takes a lot of time to repeat the measurement to complete the required test.

有鑑於此,本創作為了解決上述問題而提供一種快速三維共軛焦光譜成像裝置,可有效的縮短測試時間。In view of this, the present invention provides a fast three-dimensional conjugate focal-spectrum imaging device for solving the above problems, which can effectively shorten the test time.

本創作之快速三維共軛焦光譜成像裝置包括一第一光源、一第二光源、一物鏡、一光譜分析儀、一光電倍增管偵測器及一電荷耦合元件。第一光源發出一第一光束射向一測試樣品,部份的第一光束將被測試樣品反射,使一第一反射光束射出測試樣品,測試樣品可被激發出之一拉曼散射光。第二光源設置於第一光源之一側,第二光源發出一第二光束射向測試樣品,部份的第二光束將被測試樣品反射,使一第二反射光束射出 測試樣品。物鏡設置於測試樣品之一側,使第一光束及第二光束先穿透此物鏡後再入射測試樣品,物鏡可沿著第一光束之前進方向前後移動。光譜分析儀設置於第一光源之一側,用以接收拉曼散射光,以測量測試樣品之一拉曼散射光譜。光電倍增管偵測器設置於第一光源與光譜分析儀之間,用以接收第一反射光束,以測量測試樣品之一共軛焦影像。電荷耦合元件設置於第二光源之一側,用以接收第一反射光束或第二反射光束。The fast three-dimensional conjugate focal-focus spectral imaging device of the present invention comprises a first light source, a second light source, an objective lens, an optical spectrum analyzer, a photomultiplier tube detector and a charge coupled component. The first light source emits a first light beam directed toward a test sample, and a portion of the first light beam is reflected by the test sample, causing a first reflected light beam to exit the test sample, and the test sample can be excited to emit one of the Raman scattered light. The second light source is disposed on one side of the first light source, the second light source emits a second light beam to the test sample, and part of the second light beam is reflected by the test sample to emit a second reflected light beam testing sample. The objective lens is disposed on one side of the test sample, so that the first beam and the second beam first penetrate the objective lens and then enter the test sample, and the objective lens can move back and forth along the forward direction of the first beam. The spectrum analyzer is disposed on one side of the first light source for receiving Raman scattered light to measure a Raman scattering spectrum of one of the test samples. The photomultiplier tube detector is disposed between the first light source and the spectrum analyzer for receiving the first reflected light beam to measure a conjugate focal image of the test sample. The charge coupled component is disposed on one side of the second light source for receiving the first reflected beam or the second reflected beam.

其中測試樣品可被第二光源激發出之一螢光。Wherein the test sample can be excited by the second light source to emit a fluorescent light.

其中第一光源為一可見光雷射。The first light source is a visible light laser.

其中第一光源為一近紅外光雷射。The first source is a near-infrared laser.

其中第二光源為一白光源。The second light source is a white light source.

其中第二光源為一紅光發光二極體(LED),或為一綠光發光二極體(LED),或為一藍光發光二極體(LED)。The second light source is a red light emitting diode (LED), or a green light emitting diode (LED), or a blue light emitting diode (LED).

本創作之快速三維共軛焦光譜成像裝置可更包括一二維掃描振鏡(2-D Galvanometer Scanner),此二維掃描振鏡(2-D Galvanometer Scanner)設置於第一光束之光學路徑中,使第一光束、第一反射光束及拉曼散射光入射此二維掃描振鏡(2-D Galvanometer Scanner)後改變第一光束、第一反射光束及拉曼散射光之光學路徑。The fast three-dimensional conjugate focal-focus imaging device of the present invention further includes a 2-D Galvanometer Scanner, which is disposed in the optical path of the first beam. The first beam, the first reflected beam, and the Raman scattered light are incident on the 2-D Galvanometer Scanner to change the optical paths of the first beam, the first reflected beam, and the Raman scattered light.

其中可更包括一掃描透鏡及一調整透鏡設置於第一光束之光學路徑中,使經由二維掃描振鏡(2-D Galvanometer Scanner)反射的第一光束先穿透掃描透鏡後再穿透調整透鏡,此掃描透鏡可沿著與第一光束之前進方向垂直的方向前後移動,此調整透鏡可沿著第一光束之前進方向前後移動。The method further includes a scanning lens and an adjusting lens disposed in the optical path of the first beam, so that the first light beam reflected by the 2-D Galvanometer Scanner penetrates the scanning lens and then penetrates and adjusts a lens that can move back and forth in a direction perpendicular to the forward direction of the first beam, the adjustment lens being movable back and forth along the forward direction of the first beam.

本創作之快速三維共軛焦光譜成像裝置可更包括一邊界濾光片(Edge Filter)設置於光譜分析儀之一側且位於第一光束之光學路徑中,此邊界濾光片(Edge Filter)反射第一光束及第一反射光束,但是允許拉曼散射光穿透後再入射光譜分析儀。The fast three-dimensional conjugate focal-focus imaging device of the present invention further includes an edge filter disposed on one side of the spectrum analyzer and located in an optical path of the first beam, the boundary filter (Edge Filter) The first beam and the first reflected beam are reflected, but the Raman scattered light is allowed to pass through and then incident on the spectrum analyzer.

本創作之快速三維共軛焦光譜成像裝置可更包括一針孔設置於光譜分析儀之一側,使拉曼散射光先通過此針孔後再入射光譜分析儀,針孔之孔徑大小可調整。The fast three-dimensional conjugate focal-focus imaging device of the present invention further comprises a pinhole disposed on one side of the spectrum analyzer, so that the Raman scattered light passes through the pinhole and then enters the spectrum analyzer, and the aperture size of the pinhole can be adjusted. .

本創作之快速三維共軛焦光譜成像裝置可更包括一針孔設置於光電倍增管偵測器(PMT Detector)之一側,使第一反射光束先通過此針孔後再入射光電倍增管偵測器(PMT Detector),針孔之孔徑大小可調整。The fast three-dimensional conjugate focal-spectrum imaging device of the present invention further comprises a pinhole disposed on one side of the photomultiplier tube detector (PMT Detector), so that the first reflected beam passes through the pinhole first and then enters the photomultiplier tube to detect PMT Detector, the aperture size of the pinhole can be adjusted.

100‧‧‧快速三維共軛焦光譜成像裝置100‧‧‧fast three-dimensional conjugate focal-spectrum imaging device

10‧‧‧第一光源10‧‧‧First light source

20‧‧‧第二光源20‧‧‧second light source

30‧‧‧光電倍增管偵測器30‧‧‧Photomultiplier Tube Detector

40‧‧‧光譜分析儀40‧‧‧Spectrum Analyzer

50‧‧‧電荷耦合元件50‧‧‧Charge-coupled components

61‧‧‧反射鏡61‧‧‧Mirror

62‧‧‧分光鏡62‧‧‧beam splitter

63‧‧‧邊界濾光片63‧‧‧Boundary Filters

64‧‧‧反射鏡64‧‧‧Mirror

65‧‧‧分光鏡65‧‧‧beam splitter

66‧‧‧分光鏡66‧‧‧beam splitter

67‧‧‧二維掃描振鏡67‧‧‧Two-dimensional scanning galvanometer

68‧‧‧掃描透鏡68‧‧‧Scan lens

69‧‧‧透鏡69‧‧‧ lens

70‧‧‧調整透鏡70‧‧‧Adjustment lens

71‧‧‧針孔71‧‧‧ pinhole

72‧‧‧針孔72‧‧‧ pinhole

81‧‧‧物鏡81‧‧‧ objective lens

82‧‧‧移動平台82‧‧‧Mobile platform

91‧‧‧測試樣品91‧‧‧Test samples

92‧‧‧移動平台92‧‧‧Mobile platform

10T‧‧‧第一光束10T‧‧‧First beam

10R‧‧‧第一反射光束10R‧‧‧First reflected beam

SRS‧‧‧拉曼散射光SRS‧‧‧ Raman scattered light

20T‧‧‧第二光束20T‧‧‧second beam

20F‧‧‧螢光20F‧‧‧Fluorescent

第1圖係本創作之快速三維共軛焦光譜成像裝置之一實施例之示意圖。Figure 1 is a schematic illustration of one embodiment of a fast three-dimensional conjugate focal-focus imaging device of the present invention.

第2圖係第1圖之第一光源所發出之第一光束之光學路徑示意圖。Fig. 2 is a schematic view showing the optical path of the first light beam emitted by the first light source of Fig. 1.

第3圖係本創作之快速三維共軛焦光譜成像裝置之一實施例之拉曼散射光之光學路徑示意圖。Figure 3 is a schematic illustration of the optical path of Raman scattered light in one embodiment of the fast three-dimensional conjugate focal-focus imaging apparatus of the present invention.

第4圖係本創作之快速三維共軛焦光譜成像裝置之一實施例之共軛焦影像之光學路徑示意圖。Figure 4 is a schematic illustration of the optical path of a conjugate focal image of one embodiment of the fast three-dimensional conjugate focal-focus imaging device of the present invention.

第5圖係本創作之快速三維共軛焦光譜成像裝置之一實施例之螢光之光學路徑示意圖。Figure 5 is a schematic illustration of the optical path of the fluorescent light of one embodiment of the fast three-dimensional conjugate focal-focus imaging device of the present invention.

請參閱第1圖,第1圖係本創作之快速三維共軛焦光譜成像 裝置之一實施例之示意圖。快速三維共軛焦光譜成像裝置100包括一第一光源10、一第二光源20、一光電倍增管偵測器(PMT Detector)30、一光譜分析儀40、一電荷耦合元件(CCD)50、一反射鏡61、一分光鏡62、一邊界濾光片(Edge Filter)63、一反射鏡64、一分光鏡65、一分光鏡66、一二維掃描振鏡(2-D Galvanometer Scanner)67、一掃描透鏡68、一透鏡69、一調整透鏡70、一針孔71、一針孔72、一物鏡81、一移動平台82及一移動平台92。第一光源10可為一可見光雷射、或可為一近紅外光雷射,第一光源10發出一第一光束(未圖示),此第一光束(未圖示)最後射向一測試樣品(未圖示)。第二光源20可為一白光源、或可為一紅光發光二極體(LED)、或可為一綠光發光二極體(LED)、或可為一藍光發光二極體(LED),第二光源20發出一第二光束(未圖示),此第二光束(未圖示)最後射向測試樣品(未圖示)。二維掃描振鏡(2-D Galvanometer Scanner)67可利用兩片反射鏡轉動,以達到將入射的光束於平面掃描(即2-D掃描)之目的。分光鏡62可於如第1圖所示之垂直方向移動。掃描透鏡68可於如第1圖所示之垂直方向移動。調整透鏡70可於如第1圖所示之垂直方向移動。針孔71及針孔72之孔徑大小可依測量需求調整。移動平台82用於置放物鏡81且可於空間中的X軸、Y軸、Z軸方向移動,使物鏡81於空間中的X軸、Y軸、Z軸方向移動。移動平台92用於置放測試樣品(未圖示)且可於空間中的X軸、Y軸、Z軸方向移動,使測試樣品(未圖示)於空間中的X軸、Y軸、Z軸方向移動。Please refer to Figure 1. Figure 1 shows the fast three-dimensional conjugate focal-focus imaging of this creation. A schematic diagram of one embodiment of the device. The fast three-dimensional conjugate focal-spectrum imaging device 100 includes a first light source 10, a second light source 20, a photomultiplier tube detector (PMT Detector) 30, a spectrum analyzer 40, and a charge coupled device (CCD) 50. A mirror 61, a beam splitter 62, an edge filter 63, a mirror 64, a beam splitter 65, a beam splitter 66, and a 2-D Galvanometer Scanner 67 A scanning lens 68, a lens 69, an adjustment lens 70, a pinhole 71, a pinhole 72, an objective lens 81, a moving platform 82 and a moving platform 92. The first light source 10 can be a visible light laser, or can be a near infrared light laser. The first light source 10 emits a first light beam (not shown), and the first light beam (not shown) is finally directed to a test. Sample (not shown). The second light source 20 can be a white light source, or can be a red light emitting diode (LED), or can be a green light emitting diode (LED), or can be a blue light emitting diode (LED). The second light source 20 emits a second light beam (not shown) which is ultimately directed toward the test sample (not shown). The 2-D Galvanometer Scanner 67 can be rotated using two mirrors to achieve the purpose of scanning the incident beam in a plane (ie 2-D scan). The dichroic mirror 62 is movable in the vertical direction as shown in Fig. 1. The scanning lens 68 is movable in the vertical direction as shown in Fig. 1. The adjustment lens 70 is movable in the vertical direction as shown in Fig. 1. The aperture size of the pinhole 71 and the pinhole 72 can be adjusted according to measurement requirements. The moving platform 82 is for placing the objective lens 81 and is movable in the X-axis, Y-axis, and Z-axis directions in the space, and moves the objective lens 81 in the X-axis, Y-axis, and Z-axis directions in the space. The moving platform 92 is for placing a test sample (not shown) and is movable in the X-axis, the Y-axis, and the Z-axis direction in the space, so that the test sample (not shown) is in the X-axis, the Y-axis, and the Z in the space. Move in the direction of the axis.

底下將更進一步詳細說明快速三維共軛焦光譜成像裝置100測量共軛焦光譜及共軛焦影像時之過程與其對應之光學路徑。The process of measuring the conjugate focal length spectrum and the conjugate focal image and the corresponding optical path of the fast three-dimensional conjugate focal-spectrum imaging apparatus 100 will be further described in detail below.

請參閱第2圖,第2圖係第1圖之第一光源所發出之第一光 束之光學路徑示意圖。當欲測量測試樣品之共軛焦光譜或共軛焦影像時,需先將一測試樣品91放置於移動平台92之上,啟動第一光源10,第一光源10發出一第一光束10T,第一光束10T先射向反射鏡61後經反射鏡61反射,再射向邊界濾光片(Edge Filter)63,邊界濾光片(Edge Filter)63將使入射的第一光束10T反射,再射向二維掃描振鏡(2-D Galvanometer Scanner)67,二維掃描振鏡(2-D Galvanometer Scanner)67將使入射的第一光束10T反射,再射向掃描透鏡68且穿透掃描透鏡68,再射向反射鏡64後經反射鏡64反射,再射向調整透鏡70且穿透調整透鏡70,再射向分光鏡66後經分光鏡66部份反射,再射向物鏡81且穿透物鏡81,最後入射測試樣品91。測試樣品91可將入射的第一光束10T部份反射,使一第一反射光束10R射出測試樣品91,再入射物鏡81且穿透物鏡81,再射向分光鏡66且部份穿透分光鏡66,再射向分光鏡65後經分光鏡65部份反射,再射向透鏡69且穿透透鏡69,最後入射電荷耦合元件(CCD)50,即可由電荷耦合元件(CCD)50擷取測試樣品91之影像,再經由調整移動平台92之位置,使第一光束10T準確的入射所欲照射的位置。Please refer to FIG. 2, which is the first light emitted by the first light source of FIG. Schematic diagram of the optical path of the beam. When the conjugate focal length spectrum or the conjugate focal image of the test sample is to be measured, a test sample 91 is first placed on the moving platform 92 to activate the first light source 10, and the first light source 10 emits a first light beam 10T. A beam 10T is first incident on the mirror 61, reflected by the mirror 61, and then directed to the edge filter 63. The edge filter 63 will reflect the incident first beam 10T and re-shoot. To the 2-D Galvanometer Scanner 67, the 2-D Galvanometer Scanner 67 will reflect the incident first beam 10T, then strike the scanning lens 68 and penetrate the scanning lens 68. And then reflected by the mirror 64, reflected by the mirror 64, and then directed to the adjustment lens 70 and penetrated the adjustment lens 70, and then directed to the beam splitter 66, then partially reflected by the beam splitter 66, and then directed to the objective lens 81 and penetrated The objective lens 81 is finally incident on the test sample 91. The test sample 91 can partially reflect the incident first light beam 10T, and a first reflected light beam 10R is emitted from the test sample 91, then enters the objective lens 81 and penetrates the objective lens 81, and then faces the beam splitter 66 and partially penetrates the beam splitter. 66, after being directed to the beam splitter 65, partially reflected by the beam splitter 65, then directed toward the lens 69 and penetrating through the lens 69, and finally incident on the charge coupled device (CCD) 50, which can be tested by the charge coupled device (CCD) 50. The image of the sample 91, by adjusting the position of the moving platform 92, causes the first beam 10T to accurately enter the position to be illuminated.

當如第2圖所示之第一光束10T照射到測試樣品91後,測試樣品91因此將被第一光束10T激發出一拉曼散射光SRS。請參閱第3圖,第3圖係本創作之快速三維共軛焦光譜成像裝置之一實施例之拉曼散射光之光學路徑示意圖。拉曼散射光SRS之前進方向與第一光束10T入射測試樣品91的方向相反,其將先穿透物鏡81,再經分光鏡66部份反射,再射向調整透鏡70且穿透調整透鏡70,再經反射鏡64反射,再射向掃描透鏡68且穿透掃描透鏡68,再射向二維掃描振鏡(2-D Galvanometer Scanner)67且 經二維掃描振鏡(2-D Galvanometer Scanner)67反射,再射向邊界濾光片(Edge Filter)63且穿透邊界濾光片(Edge Filter)63,再穿透針孔72,最後入射光譜分析儀40,即可由光譜分析儀40測量出測試樣品91之某一點(位置)所被激發出之拉曼散射光光譜,此時如果將移動平台82於如第3圖所示之水平方向移動,使物鏡81也沿著如第3圖所示之水平方向移動,則可測得測試樣品91之不同深度之共軛焦拉曼散射光光譜。如欲測量測試樣品91之某一範圍所被激發出之拉曼散射光光譜,則可啟動二維掃描振鏡(2-D Galvanometer Scanner)67之掃描功能,使入射的第一光束10T於平面掃描(即2-D掃描),再搭配掃描透鏡68及調整透鏡70於如第3圖所示之垂直方向進行連動移動,使得第一光束10T掃描測試樣品91之某一範圍,即可由光譜分析儀40測量出測試樣品91之某一範圍所被激發出之拉曼散射光光譜,此時如果將移動平台82於如第3圖所示之水平方向移動,使物鏡81也沿著如第3圖所示之水平方向移動,則可測的測試樣品91之某一範圍之不同深度之共軛焦拉曼散射光光譜,即為測試樣品91之3維共軛焦拉曼散射光光譜。When the first light beam 10T as shown in Fig. 2 is irradiated to the test sample 91, the test sample 91 is thus excited by the first light beam 10T to a Raman scattered light SRS. Please refer to FIG. 3, which is a schematic diagram of the optical path of Raman scattered light in one embodiment of the fast three-dimensional conjugate focal-focus imaging device of the present invention. The forward direction of the Raman scattered light SRS is opposite to the direction in which the first beam 10T is incident on the test sample 91, which will first penetrate the objective lens 81, and then partially reflect through the beam splitter 66, and then be directed to the adjustment lens 70 and penetrate the adjustment lens 70. And then reflected by the mirror 64, then directed to the scanning lens 68 and penetrates the scanning lens 68, and then directed to the 2-D Galvanometer Scanner 67 and It is reflected by a 2-D Galvanometer Scanner 67, then directed to an Edge Filter 63 and penetrates the Edge Filter 63, penetrates the pinhole 72, and finally enters. The spectrum analyzer 40, that is, the spectrum of the Raman scattered light excited by a certain point (position) of the test sample 91 can be measured by the spectrum analyzer 40, if the moving platform 82 is moved in the horizontal direction as shown in FIG. By moving, the objective lens 81 is also moved in the horizontal direction as shown in Fig. 3, and the conjugated focal Raman scattered light spectrum of the different depths of the test sample 91 can be measured. To measure the spectrum of the Raman scattered light excited by a certain range of the test sample 91, the scanning function of the 2-D Galvanometer Scanner 67 can be activated to make the incident first beam 10T flat. The scanning (ie, 2-D scanning) is further combined with the scanning lens 68 and the adjusting lens 70 to move in the vertical direction as shown in FIG. 3, so that the first beam 10T scans a certain range of the test sample 91, which can be analyzed by spectral analysis. The meter 40 measures the spectrum of the Raman scattered light excited by a certain range of the test sample 91. At this time, if the moving platform 82 is moved in the horizontal direction as shown in FIG. 3, the objective lens 81 is also along the third level. In the horizontal direction shown in the figure, the conjugated focal Raman scattered light spectrum of a certain depth of a certain range of the test sample 91 can be measured, that is, the 3-dimensional conjugated focal Raman scattered light spectrum of the test sample 91.

請參閱第4圖,第4圖係本創作之快速三維共軛焦光譜成像裝置之一實施例之共軛焦影像之光學路徑示意圖。當測試樣品91之拉曼散射光光譜測量完成後,即可將分光鏡62於如第3圖所示之位置移動至如第4圖所示之位置,以擷取試樣品91之共軛焦影像。當如第4圖所示之第一光束10T最後經物鏡81聚焦照射到測試樣品91後,測試樣品91可將入射的第一光束10T部份反射,使第一反射光束10R射出測試樣品91,再入射物鏡81且穿透物鏡81,再經分光鏡66部份反射,再射向調整透鏡70且穿透調整透鏡70,再經反射鏡64反射,再射向掃描透鏡68且穿透掃描透鏡 68,再射向二維掃描振鏡(2-D Galvanometer Scanner)67且經二維掃描振鏡(2-D Galvanometer Scanner)67反射,再射向邊界濾光片(Edge Filter)63,邊界濾光片(Edge Filter)63再將第一反射光束10R反射,再射向分光鏡62,分光鏡62再將部份的第一反射光束10R反射使其穿透針孔71,最後入射光電倍增管偵測器(PMT Detector)30,即可由光電倍增管偵測器(PMT Detector)30擷取測試樣品91之某一點(位置)之共軛焦影像,此時如果將移動平台82於如第4圖所示之水平方向移動,使物鏡81也沿著如第4圖所示之水平方向移動,則可測得測試樣品91之不同深度之共軛焦影像。如欲測量測試樣品91之某一範圍之共軛焦影像,則可啟動二維掃描振鏡(2-D Galvanometer Scanner)67之掃描功能,使入射的第一光束10T於平面掃描(即2-D掃描),再搭配掃描透鏡68及調整透鏡70於如第4圖所示之垂直方向進行連動移動,使得第一光束10T掃描測試樣品91之某一範圍,即可由光電倍增管偵測器(PMT Detector)30測量出測試樣品91之某一範圍之共軛焦影像,此時如果將移動平台82於如第4圖所示之水平方向移動,使物鏡81也沿著如第4圖所示之水平方向移動,則可測的測試樣品91之某一範圍之不同深度之共軛焦影像,即為測試樣品91之3維共軛焦影像。Please refer to FIG. 4, which is a schematic diagram showing the optical path of the conjugate focal image of one embodiment of the fast three-dimensional conjugate focal-focus imaging device of the present invention. After the measurement of the Raman scattered light spectrum of the test sample 91 is completed, the beam splitter 62 can be moved to the position shown in FIG. 4 at the position shown in FIG. 3 to capture the conjugate focus of the test sample 91. image. When the first light beam 10T as shown in FIG. 4 is finally focused and irradiated to the test sample 91 via the objective lens 81, the test sample 91 can partially reflect the incident first light beam 10T, and the first reflected light beam 10R is emitted from the test sample 91. The object 81 is incident on the objective lens 81 and penetrates the objective lens 81, and then partially reflected by the beam splitter 66, and then directed to the adjustment lens 70 and penetrates the adjustment lens 70, and then reflected by the mirror 64, and then directed to the scanning lens 68 and penetrates the scanning lens. 68, and then directed to a 2-D Galvanometer Scanner 67 and reflected by a 2-D Galvanometer Scanner 67, and then directed to an Edge Filter 63, boundary filter The edge filter 63 reflects the first reflected beam 10R and then reflects the beam to the spectroscope 62. The spectroscope 62 reflects a portion of the first reflected beam 10R to penetrate the pinhole 71, and finally enters the photomultiplier tube. A detector (PMT Detector) 30, which can capture a conjugate focal image of a certain point (position) of the test sample 91 by a photomultiplier tube detector (PMT Detector) 30, if the mobile platform 82 is as in the fourth The horizontal movement shown in the figure causes the objective lens 81 to also move in the horizontal direction as shown in Fig. 4, and the conjugate focal image of the different depths of the test sample 91 can be measured. If a certain range of conjugate focal images of the test sample 91 are to be measured, the scanning function of the 2-D Galvanometer Scanner 67 can be activated to scan the incident first beam 10T in a plane (ie, 2- D scan), in conjunction with the scanning lens 68 and the adjustment lens 70 in the vertical direction as shown in FIG. 4, so that the first beam 10T scans a certain range of the test sample 91, that is, the photomultiplier tube detector ( The PMT Detector 30 measures a range of conjugate focal images of the test sample 91. If the moving platform 82 is moved in the horizontal direction as shown in FIG. 4, the objective lens 81 is also shown as shown in FIG. In the horizontal direction, the conjugate focal image of a certain depth of a certain range of the test sample 91 that can be measured is the 3-dimensional conjugate focal image of the test sample 91.

本創作之快速三維共軛焦光譜成像裝置也可用於測量螢光影像。請參閱第5圖,第5圖係本創作之快速三維共軛焦光譜成像裝置之一實施例之螢光之光學路徑示意圖。第二光源20發出一第二光束20T,接著依序穿透分光鏡65、分光鏡66、物鏡81,最後照射於測試樣品91,測試樣品91將被激發出一螢光20F,此螢光20F之前進方向與第二光束20T入射測試樣品91的方向相反,其將先穿透物鏡81,再部份穿透分光鏡66,再 射向分光鏡65,再部份經分光鏡65反射,再射向透鏡69且穿透透鏡69,最後入射電荷耦合元件(CCD)50,即可由電荷耦合元件(CCD)50測量出測試樣品91之螢光影像,當第二光源20為紅光發光二極體(LED)時,則可測得測試樣品91之紅光螢光影像,當第二光源20為綠光發光二極體(LED)時,則可測得測試樣品91之綠光螢光影像,當第二光源20為藍光發光二極體(LED)時,則可測得測試樣品91之藍光螢光影像。如果第二光源20為白光源時,測試樣品91可將部份的白光反射,但是無法被激發出螢光,此時由電荷耦合元件(CCD)50所測量到的為測試樣品91之可見光影像。The fast three-dimensional conjugate focal-focus imaging device of this creation can also be used to measure fluorescent images. Please refer to FIG. 5, which is a schematic diagram of the optical path of the fluorescent light of one embodiment of the fast three-dimensional conjugate focal-focus imaging device of the present invention. The second light source 20 emits a second light beam 20T, and then sequentially passes through the beam splitter 65, the beam splitter 66, the objective lens 81, and finally irradiates the test sample 91, and the test sample 91 is excited to emit a fluorescent light 20F, the fluorescent light 20F The forward direction is opposite to the direction in which the second beam 20T is incident on the test sample 91, which will first penetrate the objective lens 81 and then partially penetrate the beam splitter 66, and then The beam is directed to the beam splitter 65, and then partially reflected by the beam splitter 65, and then directed toward the lens 69 and penetrates the lens 69. Finally, the charge coupled device (CCD) 50 is incident, and the test sample 91 can be measured by the charge coupled device (CCD) 50. The fluorescent image, when the second light source 20 is a red light emitting diode (LED), the red fluorescent image of the test sample 91 can be measured, and when the second light source 20 is a green light emitting diode (LED) When the green light fluorescence image of the test sample 91 is measured, when the second light source 20 is a blue light emitting diode (LED), the blue fluorescent image of the test sample 91 can be measured. If the second light source 20 is a white light source, the test sample 91 can reflect part of the white light, but cannot be excited by the fluorescent light. At this time, the visible light image of the test sample 91 is measured by the charge coupled device (CCD) 50. .

雖然本創作已以較佳實施例揭露如上,然其並非用以限定本創作,任何於其所屬技術領域中具有通常知識者,在不脫離本創作之精神和範圍內,仍可作些許的更動與潤飾,因此本創作之保護範圍當視後附之申請專利範圍所界定者為準。Although the present invention has been disclosed in the above preferred embodiments, it is not intended to limit the present invention, and any person having ordinary knowledge in the technical field can make some changes without departing from the spirit and scope of the present invention. And the retouching, therefore, the scope of protection of this creation is subject to the definition of the scope of the patent application attached.

100‧‧‧快速三維共軛焦光譜成像裝置100‧‧‧fast three-dimensional conjugate focal-spectrum imaging device

10‧‧‧第一光源10‧‧‧First light source

20‧‧‧第二光源20‧‧‧second light source

30‧‧‧光電倍增管偵測器30‧‧‧Photomultiplier Tube Detector

40‧‧‧光譜分析儀40‧‧‧Spectrum Analyzer

50‧‧‧電荷耦合元件50‧‧‧Charge-coupled components

61‧‧‧反射鏡61‧‧‧Mirror

62‧‧‧分光鏡62‧‧‧beam splitter

63‧‧‧邊界濾光片63‧‧‧Boundary Filters

64‧‧‧反射鏡64‧‧‧Mirror

65‧‧‧分光鏡65‧‧‧beam splitter

66‧‧‧分光鏡66‧‧‧beam splitter

67‧‧‧二維掃描振鏡67‧‧‧Two-dimensional scanning galvanometer

68‧‧‧掃描透鏡68‧‧‧Scan lens

69‧‧‧透鏡69‧‧‧ lens

70‧‧‧調整透鏡70‧‧‧Adjustment lens

71‧‧‧針孔71‧‧‧ pinhole

72‧‧‧針孔72‧‧‧ pinhole

81‧‧‧物鏡81‧‧‧ objective lens

82‧‧‧移動平台82‧‧‧Mobile platform

92‧‧‧移動平台92‧‧‧Mobile platform

Claims (11)

一種快速三維共軛焦光譜成像裝置,包括:一第一光源,該第一光源發出一第一光束射向一測試樣品,部份的該第一光束被該測試樣品反射,使一第一反射光束射出該測試樣品,該測試樣品可被激發出之一拉曼散射光;一第二光源設置於該第一光源之一側,該第二光源發出一第二光束射向該測試樣品,部份的該第二光束被該測試樣品反射,使一第二反射光束射出該測試樣品;一物鏡設置於該測試樣品之一側,使該第一光束以及該第二光束先穿透該物鏡後再入射該測試樣品,該物鏡可沿著該第一光束之前進方向前後移動;一光譜分析儀設置於該第一光源之一側,用以接收該拉曼散射光,以測量該測試樣品之一拉曼散射光譜;一光電倍增管偵測器(PMT Detector)設置於該第一光源與該光譜分析儀之間,用以接收該第一反射光束,以測量該測試樣品之一共軛焦影像;以及一電荷耦合元件(CCD)設置於該第二光源之一側,用以接收該第一反射光束或該第二反射光束。 A fast three-dimensional conjugate focal-focus spectral imaging apparatus includes: a first light source, the first light source emits a first light beam directed toward a test sample, and a portion of the first light beam is reflected by the test sample to make a first reflection The light beam emits the test sample, the test sample can be excited to emit one of the Raman scattered light; a second light source is disposed on one side of the first light source, and the second light source emits a second light beam to the test sample, the second light source The second light beam is reflected by the test sample, and a second reflected light beam is emitted from the test sample; an objective lens is disposed on one side of the test sample, so that the first light beam and the second light beam first penetrate the objective lens Re-injecting the test sample, the objective lens can move back and forth along the forward direction of the first light beam; a spectrum analyzer is disposed on one side of the first light source for receiving the Raman scattered light to measure the test sample a Raman scattering spectrum; a photomultiplier tube detector (PMT Detector) is disposed between the first light source and the spectrum analyzer for receiving the first reflected beam to measure one of the test samples Image; and a charge coupled device (CCD) is provided on a side of the second light source, for receiving the first reflected beam or the second reflected beam. 如申請專利範圍第1項所述之快速三維共軛焦光譜成像裝置,其中該測試樣品可被該第二光源激發出之一螢光。 The fast three-dimensional conjugate focal-focus spectral imaging device of claim 1, wherein the test sample is excited by the second light source to emit fluorescence. 如申請專利範圍第1項所述之快速三維共軛焦光譜成像裝置,其中該第一光源為一可見光雷射。 The fast three-dimensional conjugate focal-focus spectral imaging device of claim 1, wherein the first light source is a visible light laser. 如申請專利範圍第1項所述之快速三維共軛焦光譜成像裝置,其中該第一光源為一近紅外光雷射。 The fast three-dimensional conjugate focal-focus spectral imaging device of claim 1, wherein the first light source is a near-infrared laser. 如申請專利範圍第1項所述之快速三維共軛焦光譜成像裝置,其中該第二光源為一白光源。 The fast three-dimensional conjugate focal-focus spectral imaging device of claim 1, wherein the second light source is a white light source. 如申請專利範圍第1項所述之快速三維共軛焦光譜成像裝置,其中該第二光源為一紅光發光二極體(LED),或為一綠光發光二極體(LED),或為一藍光發光二極體(LED)。 The fast three-dimensional conjugate focal-spectrum imaging device of claim 1, wherein the second light source is a red light emitting diode (LED) or a green light emitting diode (LED), or It is a blue light emitting diode (LED). 如申請專利範圍第1項所述之快速三維共軛焦光譜成像裝置,其更包括一二維掃描振鏡(2-D Galvanometer Scanner),該二維掃描振鏡(2-D Galvanometer Scanner)設置於該第一光束之光學路徑中,使該第一光束、該第一反射光束以及該拉曼散射光入射該二維掃描振鏡(2-D Galvanormeter Scanner)後改變該第一光束、該第一反射光束及該拉曼散射光之光學路徑。 The fast three-dimensional conjugate focal-focus spectral imaging device of claim 1, further comprising a 2-D Galvanometer Scanner, the 2-D Galvanometer Scanner setting In the optical path of the first light beam, the first light beam, the first reflected light beam, and the Raman scattered light are incident on the two-dimensional scanning galvanometer (2-D Galvanormeter Scanner), and the first light beam is changed. A reflected beam and an optical path of the Raman scattered light. 如申請專利範圍第7項所述之快速三維共軛焦光譜成像裝置,其更包括一掃描透鏡以及一調整透鏡設置於該第一光束之光學路徑中,使經由該二維掃描振鏡(2-D Galvanometer Scanner)反射的該第一光束先穿透該掃描透鏡後再穿透該調整透鏡,該掃描透鏡可沿著與該第一光束之前進方向垂直的方向前後移動,該調整透鏡可沿著該第一光束之前進方向前後移動。 The fast three-dimensional conjugate focal-focus imaging device of claim 7, further comprising a scanning lens and an adjustment lens disposed in the optical path of the first beam, such that the two-dimensional scanning galvanometer (2) -D Galvanometer Scanner) the first beam reflected first penetrates the scanning lens and then penetrates the adjustment lens, and the scanning lens can move back and forth along a direction perpendicular to the forward direction of the first beam, the adjustment lens can be along The first beam is moved forward and backward before the forward direction. 如申請專利範圍第1項所述之快速三維共軛焦光譜成像裝置,其更包括一邊界濾光片(Edge Filter)設置於該光譜分析儀之一側且位於該第一光束之光學路徑中,該邊界濾光片(Edge Filter)反射該第一光束以及該第一反射光束,但是允許該拉曼散射光穿透後再入射該光譜分析儀。 The fast three-dimensional conjugate focal-focus spectral imaging device of claim 1, further comprising an edge filter disposed on one side of the optical spectrum analyzer and located in an optical path of the first light beam. The edge filter reflects the first beam and the first reflected beam, but allows the Raman scattered light to pass through and then enter the spectrum analyzer. 如申請專利範圍第1項所述之快速三維共軛焦光譜成像裝置,其更包括一針孔設置於該光譜分析儀之一側,使該拉曼散射光先通過該針孔後再入射該光譜分析儀,該針孔之孔徑大小可調整。The fast three-dimensional conjugate focal-focus spectral imaging device of claim 1, further comprising a pinhole disposed on one side of the spectrum analyzer, wherein the Raman scattered light passes through the pinhole and then enters the hole. The spectrum analyzer has an adjustable aperture size. 如申請專利範圍第1項所述之快速三維共軛焦光譜成像裝置,其更包括一針孔設置於該光電倍增管偵測器(PMT Detector)之一側,使該第一反射光束先通過該針孔後再入射該光電倍增管偵測器(PMT Detector),該針孔之孔徑大小可調整。The fast three-dimensional conjugate focal-spectrum imaging device of claim 1, further comprising a pinhole disposed on one side of the photomultiplier detector (PMT Detector), so that the first reflected beam passes first The pinhole is then incident on the photomultiplier tube detector (PMT Detector), and the aperture size of the pinhole can be adjusted.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107037016A (en) * 2016-02-04 2017-08-11 北京世纪桑尼科技有限公司 A kind of confocal optical scanner
TWI647431B (en) * 2015-11-09 2019-01-11 耐諾股份有限公司 Optical metrology apparatus and method

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI647431B (en) * 2015-11-09 2019-01-11 耐諾股份有限公司 Optical metrology apparatus and method
CN107037016A (en) * 2016-02-04 2017-08-11 北京世纪桑尼科技有限公司 A kind of confocal optical scanner

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