TWM473840U - Composition structure for inhibiting bacterial infection of gastrointestinal tract - Google Patents

Description

Composition structure for inhibiting bacterial infection of gastrointestinal tract

This work relates to a composition structure, and in particular to a composition structure for inhibiting bacterial infection of the gastrointestinal tract.

Because of the tight pace of modern life, it is easy to cause gastrointestinal discomfort, and then there is a high risk of suffering from gastrointestinal related diseases. The main cause of these diseases is the infection of gastrointestinal bacteria. Escherichia coli infection can cause hemorrhagic colitis, Staphylococcus aureus infection can cause acute gastroenteritis, Helicobacter Helicobacter Infection with pylori can cause gastroenteritis, gastric ulcer, duodenal ulcer, gastric lymphoma or stomach cancer.

At present, the clinical treatment of gastrointestinal bacterial infection is based on the administration of antibiotics to patients. For example, the treatment of Helicobacter pylori infection is mainly the use of "three-in-one therapy", which involves administering two different antibiotics to the patient, such as Clarithromycin, Amoxicillin, and IV. Tetracycline, Metronidazole, and a gastric acid secretion inhibitor, such as Omeprazole and Lansoprazole, must be administered for one week (necessary) The time can be extended to two weeks. The administration of gastric acid secretion inhibitors must last for two months (if necessary, it can be extended to four months). However, during the entire treatment period, most patients may have toxic side effects on the drug, such as: dizziness, bitterness, long tongue coating, bloating, Taste dullness, nausea, diarrhea; and, coupled with a rather lengthy treatment process, patients are prone to non-compliance with treatment and affect the cure of the disease. On the other hand, long-term use of antibiotics may cause Helicobacter pylori resistance, and the results of drug resistance not only affect the cure of the disease, but also affect the choice of drugs for future treatment.

In summary, drugs for inhibiting gastrointestinal bacterial infections generally have toxic side effects and/or drug resistance problems, so it is indeed necessary to develop a variety of different drugs to provide inhibition of gastrointestinal bacterial infections.

The purpose of this creation is to propose a novel composition structure that inhibits infection of gastrointestinal bacteria.

To achieve the above and/or other objects, the present invention proposes a composition structure for inhibiting bacterial infection of the gastrointestinal tract comprising: a host layer and an active particle. The main layer is made of a carbonaceous material, and the active particles are carried on a surface of the main body layer and are made of a group of silver, gold, aluminum, zinc, copper and titanium dioxide.

According to the present creation, the main layer actively adsorbs gastrointestinal bacteria and uses active particles to kill gastrointestinal bacteria that are adsorbed and/or unadsorbed. Through this mechanism, the composition of the present composition can effectively inhibit the infection of gastrointestinal bacteria, and can be used as a drug for inhibiting bacterial infection of the gastrointestinal tract.

(1) ‧ ‧ main body

(11)‧‧‧ holes

(2) ‧‧‧active granules

The first A to C diagrams are schematic diagrams showing the composition of the composition for inhibiting bacterial infection of the gastrointestinal tract.

The second picture is a photo of a stomach endoscope showing the gastric ulcer caused by a Helicobacter pylori The condition of the patient after 7 days of taking the composition of the present composition.

The third figure is a scanning electron micrograph (X5,000) illustrating the appearance of the composition of Preparation Example 1.

The fourth figure is a scanning electron micrograph (X1,000), which shows that the activated carbon fiber main layer in the composition structure of Preparation Example 3 can adsorb Escherichia coli.

The fifth drawing is a scanning electron micrograph (X1,000) showing that the active carbon fiber main layer in the composition structure of Preparation Example 3 can adsorb H. pylori.

An antimicrobial composition has been disclosed by the present inventors in U.S. Patent Publication No. 2007/0122463, and it is further indicated that the antimicrobial composition can actively kill microorganisms, but it is merely illustrative of the use for treating skin trauma. On the other hand, activated carbon in the antimicrobial composition has been used for gastric lavage in patients with toxic drug poisoning to adsorb toxic drugs in the stomach of the patient. Moreover, silver in the antimicrobial composition has been legally used by the European Union as an additive to the numbered E174 food coloring. In other words, both activated carbon and silver are edible in our daily lives. The creator believes that if the structure of the composition of activated carbon and silver is effective in killing gastrointestinal bacteria, the structure of the composition should inhibit the infection of gastrointestinal bacteria. Further, based on the edible characteristics of the respective components in the composition of the composition, the present inventors believe that the composition of the composition should have no problem of toxic side effects and/or drug resistance.

Thus, the present invention proposes a composition structure for inhibiting bacterial infection of the gastrointestinal tract, and the composition has a structure as shown in the first A to C, which contains a host layer (1) and an active particle (2). The main body layer (1) is made of a carbonaceous material, and the active particles (2) are a group carried on a surface of the main body layer (1) and composed of silver, gold, aluminum, zinc, copper and titanium dioxide. Made of.

As can be seen from the examples below, the composition of the present invention is a structure in which a main layer (1) made of a carbonaceous material is used to adsorb gastrointestinal bacteria such as Escherichia coli, Staphylococcus aureus or Helicobacter pylori, and the activity is reused. The granules (2) kill the gastrointestinal bacteria that are adsorbed and/or remain unadsorbed. In this way, the composition of the present composition can effectively remove gastrointestinal bacteria, but can inhibit the infection of gastrointestinal bacteria. As shown in the second figure, the composition of the present composition can also treat or prevent diseases caused by bacterial infections in the gastrointestinal tract, such as hemorrhagic colitis caused by Escherichia coli infection, acute gastroenteritis caused by Staphylococcus aureus infection, pylorus A gastric ulcer, gastroenteritis or duodenal ulcer caused by a spirochete infection.

According to the unreported data, on the 30th day after feeding the composition of the rat composition, no active granules (2) were found in the liver, kidney and blood of the rats, but only in the large A trace amount of the active granule (2) component was found in the feces discharged from the mouse. This phenomenon indicates that the active particles (2) in the composition of the present composition do not remain in the rat.

The carbonaceous material to be formed into the main layer (1) is not limited to any kind as long as the main layer (1) can adsorb gastrointestinal bacteria, and examples thereof may be, but not limited to, activated carbon fibers, activated carbon powder, charcoal, and bamboo charcoal particles. , carbon black, graphite powder, expanded graphite powder, or carbon powder made of phenolic resin or synthetic resin. Further, the specific surface area of the main layer (1) is preferably from 400 to 2,500 m ² /g. The active particles (2) are not limited to any external size as long as they can kill gastrointestinal bacteria, and the particle diameter thereof is preferably from 1 nm to 500 μm, more preferably from 5 nm to 10 μm.

As shown in the first A to C diagram, in order to promote the effect of the main layer (1) on the adsorption of gastrointestinal bacteria, the main layer (1) may have pores (11). In general, the main layer (1), in addition to adsorbing gastrointestinal bacteria, may also adsorb other substances in the individual (eg: each Vitamins or probiotics, and to avoid this, the radius of the pores (11) is preferably greater than 0 nm and not more than 2.5 nm, more preferably 0.5 to 2.3 nm.

As shown in the first A to C diagram, the body layer (1) is not limited to any shape such as a cylindrical shape, a spherical shape, a lamellar shape or other irregular shape. Moreover, the shape of the body layer (1) may be related to the carbonaceous material from which the body layer (1) is formed. For example, when the carbonaceous material is activated carbon fiber, the shape of the main layer (1) is cylindrical; when the carbonaceous material is activated carbon powder, bamboo charcoal or graphite powder, the shape of the main layer (1) is spherical; carbon When the material is charcoal, the shape of the main layer (1) is lamellar.

In order to facilitate the oral administration of the composition of the present composition to the individual, when the carbonaceous material is activated carbon fiber, the main layer (1) is characterized as follows: the length is greater than 0 mm but not more than 0.1 mm, and the diameter is greater than 0 μm. More than 10μm.

In order to avoid damage to the individual caused by the active particles (2) falling off the body layer (1) in the individual, the weight percentage of the active particles (2) is preferably 0.001 to 15 wt% based on the weight of the main layer (1). %, and the amount of precipitation of the active particles (2) in water is preferably more than 0 ppm and not more than 100 ppm.

In particular, the composition of the present invention can be carried on the surface of the body layer (1) by impregnation, spraying or electroplating which is well known in the art, or by the present inventor in the US invention patent notice. No. 7,687,433 discloses the method. Moreover, when the carbonaceous material is activated carbon fiber, polyacrylonitrile (PAN) fiber, asphalt fiber, phenolic fiber, lignin fiber or Rayon fiber may be used as a raw material, and the technical field is reused. A familiar treatment mode converts the raw materials into activated carbon fibers. A detailed manufacturing process for the composition of the present composition will be specifically described in the following examples.

The following examples are presented to further illustrate this creation.

<Preparation Example 1>

Providing a polyacrylonitrile-based activated carbon fiber cloth having the following properties: a cloth weight of 70 g/m ² , a warp and weft density of 16 pieces/in, a BET specific surface area of 1,630 m ² /g, a density of 2.09 g/m ³ , and a carbon content. It is 85 wt% and the silver content is 0 wt%.

The fiber cloth was immersed in a 0.1 M aqueous silver nitrate solution (pH 3.8) for 5 hours in a vacuum atmosphere to reduce silver ions to silver nuggets attached to the fiber cloth. The fiber cloth with the silver block is dried to remove the residual moisture.

The dried fibers were placed in a high temperature furnace filled with nitrogen at a temperature of 400 ° C for 90 minutes to crack the silver nump into silver particles dispersed on the fiber cloth.

Grinding, pulverizing and sieving the fiber cloth with silver particles to obtain a main layer of activated carbon fiber carrying silver particles (as shown in the second figure, the silver particles and the active carbon fiber main layer are collectively referred to as "composition structure" ").

The composition structure of this preparation example has the following characteristics: a BET specific surface area of about 1,200 m ² /g, a density of about 2.13 / m ³ , a carbon content of about 64 wt%, and a silver content of about 12.5% by weight; The bulk layer has the following features: a length of about 0.1 mm, a diameter of about 6.0 μm, and a pore diameter of about 2.41 nm; the silver particles in the composition have the following characteristics: the amount of precipitation in water is about 15.75 ppm.

<Preparation Example 2>

The polyacrylonitrile-based activated carbon fiber cloth used in Preparation Example 1 was immersed in a 0.1 M silver nitrate aqueous solution (pH 3.8) for 5 hours in a vacuum atmosphere to reduce silver ions to silver blocks attached to the fiber cloth. . Dry the fiber cloth with silver block to remove Its residual moisture.

The dried fibers were placed in a high temperature furnace filled with nitrogen at a temperature of 400 ° C for 90 minutes to crack the silver nump into silver particles dispersed on the fiber cloth.

The fiber cloth with silver particles was washed with water at a flow rate of 4.5 liters/min for 120 hours to remove silver particles not attached to the fiber cloth. Dry the cleaned fiber cloth.

The dried fiber cloth is ground, pulverized, and sieved to obtain an active carbon fiber main layer (silver particles and an active carbon fiber main layer collectively referred to as a "composition structure") carrying silver particles.

The composition structure of this preparation example has the following characteristics: a BET specific surface area of about 1,220 m ² /g, a density of about 2.13 / m ³ , and a silver content of about 0.03 wt%; the bulk layer in the composition structure has the following characteristics: length It is about 0.1 mm, has a diameter of about 6.0 μm, and has a pore diameter of about 2.41 nm; the silver particles in the composition structure have the following characteristics: the amount of precipitation in water is about 10.04 ppm.

<Preparation Example 3>

A phenolic activated carbon fiber felt having the following properties: a felt weight of 100 g/m ² , a BET specific surface area of 1,420 m ² /g, a density of 2.032 g/m ³ , a carbon content of 85 wt%, and a silver content of 0 wt%.

The fiber mat was immersed in a 0.001 M aqueous solution of silver nitrate (pH 6.4) for 5 hours under vacuum to reduce the silver ions to silver nuggets attached to the fiber mat. The fiber mat with the silver block is dried to remove the residual moisture.

The dried fiber mat was placed in a high temperature furnace at a temperature of 400 ° C and filled with nitrogen for 90 minutes to crack the silver pieces into silver particles dispersed on the fiber mat.

The fiber mat with the silver particles is ground, pulverized, and sieved to obtain an activated carbon fiber main layer (the silver particles and the activated carbon fiber main layer collectively referred to as a "composition structure") carrying silver particles.

The composition structure of this preparation example has the following characteristics: a BET specific surface area of about 1,380 m ² /g, a density of about 2.0425 / m ³ , a carbon content of about 75 wt%, and a silver content of about 0.072 wt%; The bulk layer has the following features: a length of about 0.1 mm, a diameter of about 6.0 μm, and a pore diameter of about 2.23 nm; the silver particles in the composition structure have the following characteristics: the amount of precipitation in water is about 13.52 ppm.

<Preparation Example 4>

The activated carbon particles made of plants were immersed in a silver nitrate aqueous solution having a concentration of 0.008 M in a vacuum atmosphere, and were rotationally mixed at 50 rpm for 2 hours to reduce silver ions to silver nuggets attached to the activated carbon particles. The activated carbon particles with silver blocks are dried to remove residual moisture.

The dried activated carbon particles were heated to 600 ° C at a heating rate of 4 ° C / min under a nitrogen atmosphere, and the temperature was maintained for about 60 minutes. Finally, the dried activated carbon particles were cooled to a chamber at a cooling rate of 10 ° C / minute. The temperature is such that the silver block is cleaved into silver particles dispersed on the activated carbon particles.

After washing the activated carbon particles to which silver particles were attached, the washed activated carbon particles were dried at 120 °C.

The dried activated carbon particles are ground, pulverized, and sieved to obtain an active carbon powder main layer (silver particles and an active carbon powder main layer collectively referred to as a "composition structure") carrying silver particles.

The composition structure of this preparation example has the following characteristics: a BET specific surface area of about 1,024 m ² /g, a carbon content of about 73.54 wt%, and a silver content of about 0.22 wt%; the main layer in the composition structure has the following characteristics: The diameter is about 0.1 mm or less and the diameter of the pores is about 2.48 nm; the silver particles in the composition structure have the following characteristics: a particle diameter of about 100 to 300 nm and a precipitation amount in water of about 30.49 ppm.

<Comparative Example 1>

The polyacrylonitrile-based activated carbon fiber cloth used in Preparation Example 1 was ground, pulverized, and sieved to obtain an active carbon fiber main layer having a length of about 0.1 mm.

<Comparative Example 2>

A polyacrylonitrile-based activated carbon fiber mat having the following properties is ground, pulverized and sieved to obtain an active carbon fiber main layer having a length of about 0.1 mm: a felt weight of 100 g/m ² and a BET specific surface area of 600 m ² /g. The pore diameter was 2.07 nm, the density was 1.932 g/m ³ , the carbon content was 86 wt%, and the silver content was 0 wt%.

<Comparative Example 3>

The activated carbon particles used in Preparation Example 4 were ground, pulverized and sieved to obtain an active carbon powder main layer having a particle diameter of about 0.1 mm or less and a BET specific surface area of about 1,135 m ² /g. The carbon content is about 84.24% by weight and the pore diameter is about 2.49 nm.

<Analysis example>

Referring to Table 1, the series shows the compositional structures of Preparation Examples 1 to 4, the activated carbon fiber main layers of Comparative Examples 1 and 2, and the activated carbon powder main layer of Comparative Example 3 for the sterilization effects of different strains. The "sterilization rate" representing the sterilization effect in the table is based on American textiles. Measured by the American Association of Textile Chemists and Colorists (ATCC) 100-1998 test method.

As can be seen from the above table, the source layer of the composition of the preparation example 1 was the same as the source layer of the comparative example 1, but since the composition of the preparation example 1 had silver particles, the preparation example 1 was The sterilizing effect of the composition structure on Escherichia coli, Helicobacter pylori, Staphylococcus aureus and Klebsiella pneumoniae was significantly better than that of the main body layer of Comparative Example 1.

As can be seen from the above table, the source layer of the composition of the preparation example 4 was the same as the source layer of the comparative example 3, but since the composition of the preparation example 4 had silver particles, the preparation example 4 was prepared. The composition of the composition is significantly better than the comparison of the sterilization effect of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Helicobacter pylori and Klebsiella pneumoniae. The main layer of Example 3.

As shown in the fourth and fifth figures, it was apparent that the host layer in the composition structure of Preparation Example 3 can adsorb Escherichia coli and Helicobacter pylori, respectively. Silver particles release silver ions, especially in water, so that silver ions can bind to the thiol group (-SH) in the adsorbed and/or unadsorbed E. coli or Helicobacter pylori enzymes, leaving the enzyme inactive. And effectively kill E. coli or Helicobacter pylori.

In combination with the above description of the examples, it was confirmed that the composition of the present composition can inhibit the infection of gastrointestinal bacteria and has the potential as a drug for inhibiting bacterial infection of the gastrointestinal tract. On the other hand, the material for forming the main layer and the active particles is edible, and therefore, the composition of the present composition, as a drug for inhibiting gastrointestinal bacterial infection, does not cause toxic side effects and/or to individuals who administer the drug. Drug resistance.

However, the above-mentioned ones are only preferred embodiments of the present invention, but the scope of the present invention cannot be limited thereto; therefore, the simple equivalent changes and modifications made by the scope of the patent application and the content of the creation manual are All should remain within the scope of this creation patent.

(1) ‧ ‧ main body

(11)‧‧‧ holes

(2) ‧‧‧active granules

Claims (9)

A composition for inhibiting bacterial infection of the gastrointestinal tract, comprising: a bulk layer made of a carbonaceous material and having pores having a radius greater than 0 nm and not exceeding 2.5 nm; and an active particle carrying It is made on a surface of the main body layer and is made up of a group consisting of silver, gold, aluminum, zinc, copper and titanium dioxide. The composition structure of claim 1, wherein the carbonaceous material is selected from the group consisting of activated carbon fiber, activated carbon powder, charcoal, bamboo charcoal, carbon black, graphite powder, expanded graphite powder, and phenolic resin or artificial A group consisting of toner made of resin. The composition structure of claim 1, wherein the body layer has a specific surface area of 400 to 2,500 m ² /g. The composition structure according to claim 1, wherein when the carbonaceous material is activated carbon fiber, the bulk layer has a cylindrical shape and a length of more than 0 nm and less than 0.1 mm. The composition structure according to claim 1, wherein when the carbonaceous material is activated carbon fiber, the body layer has a cylindrical shape and a diameter of more than 0 μm and not more than 10 μm. The composition structure according to claim 1, wherein the active particles have a particle diameter of from 1 nm to 500 μm. The composition structure according to claim 1, wherein the active particles are 0.001 to 15% by weight based on the weight of the main layer. The composition structure of claim 1, wherein the active particles are in water The amount of precipitation in the system is more than 0 ppm and not more than 100 ppm. The composition structure as described in claim 1 is for inhibiting infection of Escherichia coli, Staphylococcus aureus or Helicobacter pylori.